Objective： To find out the relationship of the expressions of proliferating cell nuclear antigen （proliferating cell nuclear antigen, PCNA）, alkaline phosphotase （alkaline phosphotase, AKP） and acid phosphotase ...Objective： To find out the relationship of the expressions of proliferating cell nuclear antigen （proliferating cell nuclear antigen, PCNA）, alkaline phosphotase （alkaline phosphotase, AKP） and acid phosphotase （acid phosphotase, ACP） with the development of mouse fore stomach cancerization. Methods： The animal models, including the various stages during the development of NIH mouse fore stomach carcinoma, were made by N-Nitrososarcosineethylester （N-Nitrososarcosineethylester, NSEE）. The mice were sacrificed on the 14th, 28th, 42nd, 56th, 70th and 84th days respectively after mice were irrigated with NSEE. The fore stomach was taken out and dissected. The methods of histopathology, immunohistochemistry and isoenzyme electrophoresis were adopted to study the dynamic changes of cell shape and expression of PCNA, AKP and ACP. Results： On the 42nd and 56th days after NSEE treatment, the expression of PCNA increased gradually along with the cancerization. Comparing with the control, there were significant differences （P〈0.05）. On the 70th and 84th days, the expression of PCNA increased further （compared with the control P〈0.01）. The activity of AKP increased gradually along with the cancerization. On the 14th, 28th, 42nd and 56th days, there were significant differences （P〈0.05）; on the 70th and 84th days, the activity of AKP increased further （P〈0.01）. The activity of ACP also increased on the 14th, 28th, 42nd and 56th days, and there were significant differences on the 70th days （P〈0.05） and on the 84th days （P〈0.01） compared with the control. Conclusion： During the carcinogenesis of NIH mouse fore stomach, the expressions of PCNA, AKP and ACP increased gradually and were consisted with the changes of cell shapes.展开更多
Objective To study the effects of electromagnetic pulse （EMP） on bone metabolism of mice in vivo. Methods Twenty-four male BALB/c mice were divided into a control group and 2 experimental groups （n=8）. The whole-b...Objective To study the effects of electromagnetic pulse （EMP） on bone metabolism of mice in vivo. Methods Twenty-four male BALB/c mice were divided into a control group and 2 experimental groups （n=8）. The whole-body of mice in experimental groups were exposed to 50 kV/m and 400kV/m EMP, 400 pulses daily for 7 consecutive days at 2 seconds intervals. Alkaline phosphotase （ALP） activity, serum calcium concentration and osteocalcin level and trabecular bone volume （BV/TV, %） were measured immediately after EMP exposure by biochemical, ELISA and morphological methods. Results The ALP activity, serum calcium concentration and osteocalcin level and BV/TV in experimental groups remained unchanged after EMP exposure. Conelusion Under our experimental conditions, EMP exposure cannot affect bone metabolism of mice in vivo.展开更多
基金This work was supported by Henan Technologies R & D Project (No. 0424420043) and Henan Zoology Key Subject.
文摘Objective： To find out the relationship of the expressions of proliferating cell nuclear antigen （proliferating cell nuclear antigen, PCNA）, alkaline phosphotase （alkaline phosphotase, AKP） and acid phosphotase （acid phosphotase, ACP） with the development of mouse fore stomach cancerization. Methods： The animal models, including the various stages during the development of NIH mouse fore stomach carcinoma, were made by N-Nitrososarcosineethylester （N-Nitrososarcosineethylester, NSEE）. The mice were sacrificed on the 14th, 28th, 42nd, 56th, 70th and 84th days respectively after mice were irrigated with NSEE. The fore stomach was taken out and dissected. The methods of histopathology, immunohistochemistry and isoenzyme electrophoresis were adopted to study the dynamic changes of cell shape and expression of PCNA, AKP and ACP. Results： On the 42nd and 56th days after NSEE treatment, the expression of PCNA increased gradually along with the cancerization. Comparing with the control, there were significant differences （P〈0.05）. On the 70th and 84th days, the expression of PCNA increased further （compared with the control P〈0.01）. The activity of AKP increased gradually along with the cancerization. On the 14th, 28th, 42nd and 56th days, there were significant differences （P〈0.05）; on the 70th and 84th days, the activity of AKP increased further （P〈0.01）. The activity of ACP also increased on the 14th, 28th, 42nd and 56th days, and there were significant differences on the 70th days （P〈0.05） and on the 84th days （P〈0.01） compared with the control. Conclusion： During the carcinogenesis of NIH mouse fore stomach, the expressions of PCNA, AKP and ACP increased gradually and were consisted with the changes of cell shapes.
基金supported by the Research Fund of National Natural Science Foundation of China (No.30800928,No.30670492)the National 863 Project (No.2006 AA02Z4C3)
文摘Objective To study the effects of electromagnetic pulse （EMP） on bone metabolism of mice in vivo. Methods Twenty-four male BALB/c mice were divided into a control group and 2 experimental groups （n=8）. The whole-body of mice in experimental groups were exposed to 50 kV/m and 400kV/m EMP, 400 pulses daily for 7 consecutive days at 2 seconds intervals. Alkaline phosphotase （ALP） activity, serum calcium concentration and osteocalcin level and trabecular bone volume （BV/TV, %） were measured immediately after EMP exposure by biochemical, ELISA and morphological methods. Results The ALP activity, serum calcium concentration and osteocalcin level and BV/TV in experimental groups remained unchanged after EMP exposure. Conelusion Under our experimental conditions, EMP exposure cannot affect bone metabolism of mice in vivo.
