Background:Skin aging has recently gained significant attention in both society and skin care research.Understanding the biological processes of photoaging caused by long-term skin exposure to ultraviolet radiation is...Background:Skin aging has recently gained significant attention in both society and skin care research.Understanding the biological processes of photoaging caused by long-term skin exposure to ultraviolet radiation is critical for preventing and treating skin aging.Therefore,it is important to identify genes related to skin photoaging and shed light on their functions.Methods:We used data from the Gene Expression Omnibus(GEO)database and conducted bioinformatics analyses to screen and extract microRNAs(miRNAs)and their downstream target genes related to skin photoaging,and to determine possible biological mechanisms of skin photoaging.Results:A total of 34 differentially expressed miRNAs and their downstream target genes potentially related to the biological process of skin photoaging were identified.Gene Ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that these target genes were enriched in pathways related to human papillomavirus infection,extracellular matrix(ECM)-receptor signaling,estrogen receptor,skin development,epidermal development,epidermal cell differentiation,keratinocyte differentiation,structural components of the ECM,structural components of the skin epidermis,and others.Conclusion:Based on the GEO database-derived findings,we determined that target genes of two miRNAs,namely miR-4667-5P-KRT79 and miR-139-5P-FOS,play an important role in skin photoaging.These observations could provide theoretical support and guidance for further research on skin aging-related biological processes.展开更多
Skin photoaging is induced and sustained by UV-induced oxidative damage,and stimulating regeneration of the UV-induced aging has remained a great challenge due to high-level oxidative stress factor(ROS)-induced chroni...Skin photoaging is induced and sustained by UV-induced oxidative damage,and stimulating regeneration of the UV-induced aging has remained a great challenge due to high-level oxidative stress factor(ROS)-induced chronic oxidative damage and inactivation of bio-macromolecule-based regeneration in oxidative photoaging micro-environment.In this study,we designed a“seed and soil”strategy to pursue a safer and more efficient way to prevent and treat photoaging by simultaneously changing UV-induced ROS-rich micro-environment into a proregenerative one(the“soil”)and providing growth factor-rich platelet lysates(PL,the“seed”)using PL-impregnated,collagen-reinforce hydrogel(PL/Col).SD rats were used to establish photoaging model by 8 weeks of UV irradiation.The effectiveness of different treatments was evaluated by making pathological sections and detecting photoaging-related indicators.Rats treated with PL/Col demonstrated a significant acceleration in skin healing and enhancement in the quality of trauma repairing.After treated with PL/Col,the rats showed smooth yellowish appearance,integral structure of skin collagen fiber and epidermis,a decrease in inflammation and a reshaped active micro-environment with reduced levels of SOD enzyme activity,GSH enzyme activity and MDA toxic products.Treatment of PL/Col in skin photoaging has shown potential anti-oxidation and anti-aging effects and is worthy of further study in related field.展开更多
Background and Objectives:Microneedling has been introduced as a new technique to address the growing concern of facial skin photoaging.Kangfuxin liquid has been found to promote the process of skin wound repair,inclu...Background and Objectives:Microneedling has been introduced as a new technique to address the growing concern of facial skin photoaging.Kangfuxin liquid has been found to promote the process of skin wound repair,including reducing inflammatory response,improving immunity and enhancing antioxidant levels.In this prospective randomized double-blinded study,we wanted to explore the clinical efficacy and safety of Kangfuxin liquid combined with microneedling in treating facial skin photoaging.Methods:57 patients with facial skin photoaging were randomly divided into two groups.The treatment group(28 cases)received microneedle therapy with Kangfuxin liquid,while the control group(29 cases)received microneedle therapy with physiological saline.The treatment interval was 4 weeks,and a total of 3 treatments were performed.Compare the VISIA scores of facial photoaging features such as wrinkles,texture,pores,spots and ultraviolet pigmentation between two groups of patients before and after treatment,Global Score for Photoaging,satisfaction evaluation,and record the occurrence of adverse reactions.Results:After treatment,the treatment group showed more significant improvement in wrinkles,texture,pores,spots and ultraviolet pigmentation,and the Global Score for Photoaging was better than the control group(P<0.05).The satisfaction rate with improving skin in the treatment group was 85.71%,which was higher than 75.86%in the control group(P<0.05).Both groups did not experience adverse reactions such as skin infection,pigmentation or hypopigmentation,scar formation,or worsening of melasma.Conclusion:Kangfuxin liquid combined with microneedling therapy has a good improvement effect on facial skin photoaging,with a low incidence of adverse reactions and high patient satisfaction.It is worthy of clinical promotion.展开更多
The objective of this study was to investigate the effect of gelatin(SG) isolated from salmon skin and its hydrolysate(SGH) on photoaging skin, and the mechanism responsible for anti-photoaging. The average molecular ...The objective of this study was to investigate the effect of gelatin(SG) isolated from salmon skin and its hydrolysate(SGH) on photoaging skin, and the mechanism responsible for anti-photoaging. The average molecular weights of SG and SGH were 65 k Da and 873 Da, respectively. The amino acid compositions of SG and SGH were similar. Both of them were abundant in hydrophobic amino acids. Twenty-five peptides were identified from SGH. SG and SGH could improve UV irradiation-induced pathological changes of macroscopical tissue texture and skin morphology. Hydroxyproline content is an indicator of matrix collagen content, SG and SGH could inhibit the decrease of hydroxyproline content in photoaging skin in a dose dependent manner. In addition, SG and SGH could alleviate UV irradiation-induced oxidative damages to skin by increasing the activities of total superoxide dismutase(T-SOD), glutathione peroxidase(GSH-Px) and catalase(CAT), increasing the content of glutathione(GSH) and decreasing the content of malonaldehyde(MDA). Moreover, SG and SGH could enhance immune regulation system by increasing the thymus index. Thus, the anti-photoaging mechanisms of SG and SGH were by inhibiting the depletion of antioxidant defense components, involving in the synthesis of collagen and enhancing the function of immune system. Besides, SGH showed a better result in protecting skin from photoaging than SG.展开更多
Dendrobium officinale is a valuable medicinal herb that is widely used in traditional Chinese medicine.The chemical constituents of D.officinale have attracted much attention and a large number of compounds have been ...Dendrobium officinale is a valuable medicinal herb that is widely used in traditional Chinese medicine.The chemical constituents of D.officinale have attracted much attention and a large number of compounds have been reported including many bibenzyl derivatives.13 bibenzyl derivatives from D.officinale were sent for molecular docking,sur-face plasmon resonance(SPR)assay and after detection of Mn-SOD and SIRT3 activities in or not in HaCaT cells,it was concluded that bibenzyl derivatives did not directly activate Mn-SOD but promoted SIRT3 proteins.In addition,HaCaT cells were irradiated with UV-B to induce an oxidative stress model in vitro to further verify the effect of bibenzyl derivatives.The results show that bibenzyl derivatives could directly bind to SIRT3,enhance the deacetylation and then activate Mn-SOD,so as to protect UV-B induced skin photoaging.展开更多
Skin is the largest organ of human body and it protects the organism from external stimuli.Skin will age as the organism ages in normal circumstances.Skin photoaging refers to the accumulation of ultraviolet radiation...Skin is the largest organ of human body and it protects the organism from external stimuli.Skin will age as the organism ages in normal circumstances.Skin photoaging refers to the accumulation of ultraviolet radiation due to long-term exposure to sunlight,which results in the premature aging of skin,wrinkles,pigmentation,skin laxity,and other signs of aging.The increase in the level of reactive oxygen species(ROS)induced by ultraviolet radiation,which causes the oxidation of cells,proteins,lipids,and other components,is an important cause of accelerated photoaging of skin.For this reason,the skin's antioxidant system will further play an antioxidant role to reduce oxidative damage by inhibiting ROS production,breaking down ROS,and degrading oxides.Therefore,it is important to understand the mechanism of skin damage caused by Ultraviolet radiation,the effects of reactive oxygen species and how the body's antioxidant system exerts its antioxidant effect.This will not only deepen the understanding of skin photoaging but also provide a scientific basis for the research on how to prevent and treat photoaging.展开更多
The skin is a major protective organ of the body. It is constantly exposed to the environment and is very resilient. But exposure to ultraviolet (UV) rays from the sun results in the production of reactive oxygen spec...The skin is a major protective organ of the body. It is constantly exposed to the environment and is very resilient. But exposure to ultraviolet (UV) rays from the sun results in the production of reactive oxygen species (ROS) and subsequent inflammatory responses that can overwhelm the innate protective mechanisms of the skin. This results in damage and premature aging. Strategies to mitigate this premature photoaging might include avoidance of sunlight. However, some sunlight exposure is beneficial to health. One notable example of this is the production of vitamin D. A more practical approach to preventing adverse effects of UV light in the skin is antioxidant supplementation. Dietary antioxidants may help control ROS propagation following UV light exposure. To further evaluate the utility of antioxidants in protecting the skin, in vitro, in vivo and human studies of three well known dietary antioxidants are reviewed and discussed. The data clearly demonstrate that vitamin C, grape seed extract and citrus bioflavonoids have the potential to reduce the damaging effects of excess sun exposure via antioxidant, anti-inflammatory and immunomodulating mechanisms. As such, regular ingestion of dietary antioxidants appears to be a useful strategy for protecting the skin against photoaging.展开更多
Physiological processes, as aerobic metabolism and inflammatory response, generate reactive oxygen species (ROS) that may induce cellular injury when their amount is increased and antioxidant defense mechanisms are ov...Physiological processes, as aerobic metabolism and inflammatory response, generate reactive oxygen species (ROS) that may induce cellular injury when their amount is increased and antioxidant defense mechanisms are overwhelmed. Also, ROS are generated following UV skin irradiation able to deplete the natural antioxidant defenses in the skin. The increase in exposure to UV may lead to photoaging and precancerous skin lesions (actinic keratosis). New antioxidant strategies in the prevention and therapy of skin lesions are urgently needed. In this study, we evaluated the antioxidant efficacy of a recombinant form of human manganese superoxide dismutase able to inhibit reactive oxygen species production in some patients affected by severe photoaging and actinic keratosis.展开更多
Photoaging is an accelerating aging process of the skin due to prolonged exposure to UV from the Sun or other sources. Herbal extracts, natural compounds, and bioactive polypeptides have widely used in cosmetic agents...Photoaging is an accelerating aging process of the skin due to prolonged exposure to UV from the Sun or other sources. Herbal extracts, natural compounds, and bioactive polypeptides have widely used in cosmetic agents for protection of the skin against photoaging. This mini review briefly summarizes topical use of selected most common medicinal herbs, naturopathic chemicals, and bioactive peptides examined for skin protection.展开更多
Skin photoaging is a complex, multifactorial process resulting in functional and structural changes of the skin, and different phenotypes from chronological skin aging are well-recognized. Ultraviolet (UV)-irradiated ...Skin photoaging is a complex, multifactorial process resulting in functional and structural changes of the skin, and different phenotypes from chronological skin aging are well-recognized. Ultraviolet (UV)-irradiated hairless mice have been used as a skin photoaging animal model. However, differences in morphology and gene expression patterns between UV-induced and chronological skin changes in this mouse model have not been fully elucidated. Here we investigated differences in histopathology and cytokine expression between UV-irradiated and non-irradiated aged hairless mice to clarify the factor(s) that differentiate photoaging from chronological skin aging phenotypes. Eight-week-old HR-1 hairless mice were divided into UV-irradiated (UV-irradiated mice) and non-irradiated (control mice) groups. Irradiation was performed three times per week for 10 weeks. In addition, 30-week-old HR-1 hairless mice were reared until 70 weeks of age without UV irradiation (aged mice). Histopathologies revealed that the flattening of dermal-epidermal junctions and epidermal thickening were observed only in UV-irradiated mice. Decreases in fine elastic fibers just beneath the epidermis, the thickening of elastic fibers in the reticular dermis, and the accumulation of glycosaminoglycans were more prominent in UV-irradiated mice as compared to non-irradiated aged mice. Quantitative PCR analyses revealed that UV-irradiated mice showed an increase in the expression of IFN-γ. In contrast, aged mice exhibited proportional up-regulation of both pro-inflammatory and anti-inflammatory cytokines. The IFN-γ/IL-4 ratio, an indicator for the balance of pro-inflammatory and anti-inflammatory cytokines, was significantly higher in UV-irradiated mice as compared to control and non-irradiated aged mice. An elevated IFN-γ/IL-4 ratio was also observed in aged senescence-accelerated mouse-prone 1 (SAMP1) mice, a spontaneous skin photoaging model we recently reported. Thus, an imbalance between pro-inflammatory and anti-inflammatory cytokines might be a key factor to differentiate photoaged skin from chronologically-aged skin.展开更多
Plastic products widespread in natural water can be broken into smaller-sized microplastics(MPs,<5 mm)under light irradiation,thermal degradation and biodegradation,posing a serious threat to aquatic ecosystems and...Plastic products widespread in natural water can be broken into smaller-sized microplastics(MPs,<5 mm)under light irradiation,thermal degradation and biodegradation,posing a serious threat to aquatic ecosystems and human health.This perspective concludes that MPs can generate reactive oxygen species(ROS)through initiation,propagation and termination steps,which can attack the polymer resulting in the photoaging and breakdown of C–C and C–H bonds under ultraviolet(UV)irradiation.Free radical generation and weathering degree of MPs depend on their physicochemical properties and environmental conditions.In general,UV irradiation and co-existed MPs can significantly accelerate MP photoaging.With plentiful chromophores carbonyl,carboxyl and benzene rings,Dissolved organic matter(DOM)mainly absorbs photons(300–500 nm)and generates hydrated electrons,^(3)DOM^(*) and ROS,which may affect MP photoaging.However,whether DOM may transfer the electron and energy to MPs under UV irradiation,affect ROS generation of MPs and their photoaging pathway are inadequately studied.More studies are needed to elucidate MP photoaging pathways and mechanisms,consider the influence of stabilization capacity,photosensitization and photoionization of DOM as well as their competitive light absorption with MPs,which provides valuable insights into the environmental behavior and ecological risk of MPs in natural water.展开更多
Objective: To evaluate whether α-ionone, an aromatic compound mainly found in raspberries, carrots,roasted almonds, fruits, and herbs, inhibits UVB-mediated photoaging and barrier dysfunction in a human epidermal ker...Objective: To evaluate whether α-ionone, an aromatic compound mainly found in raspberries, carrots,roasted almonds, fruits, and herbs, inhibits UVB-mediated photoaging and barrier dysfunction in a human epidermal keratinocyte cell line(HaCaT cells).Methods: The anti-photoaging effect of α-ionone was evaluated by detecting the expression of barrierrelated genes and matrix metalloproteinases(MMPs) in HaCaT cells. The levels of reactive oxygen species,oxidation product, antioxidant enzyme, and inflammatory factors were further analysed to underline the protective effect of α-ionone on epidermal photoaging.Results: It was found that α-ionone attenuated UVB-induced barrier dysfunction by reversing keratin 1and filaggrin in HaCaT cells. α-Ionone also reduced the protein amount of MMP-1 and mRNA expression of MMP-1 and MMP-3 in UVB-irradiated HaCaT cells, implying protective effects on extracellular matrix.Furthermore, HaCaT cells exposed to α-ionone showed significant decreases in interleukin(IL)-1β, IL-6,IL-8, and tumor necrosis factor-α as compared to UVB-irradiated HaCaT cells. α-Ionone treatment significantly inhibited the UVB-induced intracellular reactive oxygen species increase and malondialdehyde accumulation. Therefore, the beneficial effects of α-ionone on inhibiting MMPs secretion and barrier damage may be related to attenuated inflammation and oxidative stress.Conclusion: Our results highlight the protective effects of α-ionone on epidermal photoaging and promote its clinic application as a potential natural anti-photodamage agent in future.展开更多
背景组织蛋白酶 B 在房间周期，细胞外的矩阵变化和皮肤的 tumorigenesis 起一个重要作用：它是否在未知的 photoaged 皮肤遗体起一个作用。这研究试图在 vivo 并且在 vitro 在皮肤 photoaging 调查组织蛋白酶 B 的角色。组织蛋白酶 B ...背景组织蛋白酶 B 在房间周期，细胞外的矩阵变化和皮肤的 tumorigenesis 起一个重要作用：它是否在未知的 photoaged 皮肤遗体起一个作用。这研究试图在 vivo 并且在 vitro 在皮肤 photoaging 调查组织蛋白酶 B 的角色。组织蛋白酶 B 的表情与在太阳的暴露的皮肤和六个健康中国志愿者的太阳的保护的皮肤的 immunohistochemical 方法相比的方法。在紫外轻 A (UVA ) 的组织蛋白酶 B 的 mRNA 和蛋白质表示在 vitro 导致了早熟的老朽成纤维细胞被即时反向的抄写聚合酶链反应(RT-PCR ) 和西方的弄污技术检测。结果减少了组织蛋白酶 B 的表示与太阳的保护的皮肤的相比在 photoaged 皮肤被观察。在 UVA 导致的、早熟的老朽成纤维细胞，组织蛋白酶 B 的更低的表情被西方的弄污检测，在一样的房间的组织蛋白酶 B mRNA 的减少的合成被即时 RT-PCR 揭示。结果在 vitro 并且在 vivo 表明了在皮肤 photoaging 和组织蛋白酶 B 之间的重要否定关联的结论。我们建议除矩阵 metalloproteinases 和抗氧化剂酶以外，组织蛋白酶 B 涉及皮肤 photoaging 的过程因为它贡献细胞外的矩阵改变并且是在细胞的 apoptosis 和老朽的主导的朊酶。展开更多
OBJECTIVE:Chronic exposure to ultraviolet(UV) radiation induces skin photoaging(premature skin aging).UV irradiation generates reactive oxygen species(ROS),which are shown to play a pivotal role in skin photoaging.Ayu...OBJECTIVE:Chronic exposure to ultraviolet(UV) radiation induces skin photoaging(premature skin aging).UV irradiation generates reactive oxygen species(ROS),which are shown to play a pivotal role in skin photoaging.Ayurveda is a holistic traditional medical system,and Chyawanprash is one of the most popular formulations in Ayurveda.Since maintenance of the function and appearance of skin is important,we examined whether Chyawanprash has a protective effect on skin photoaging.METHODS:To examine the effect of Chyawanprash on skin photoaging,hairless mice were administered with Chyawanprash in drinking water for 3 weeks,and then repeatedly exposed to ultraviolet light B(UVB) irradiation(225 or 450 mJ/cm2) to induce skin photoaging.To further examine the function of Chyawanprash,its effects were examined in cells cultured In vitro.Chyawanprash was added in culture medium,and examined for the effect on the growth of human keratinocytes,and for the ability to eliminate ROS which generated by paraquat(50 pmol/L) in HeLa cells.RESULTS:UVB irradiation caused symptoms such as rough skin,erythema,and edema on the skin in hairless mice,but administration of Chyawanprash relieved these symptoms.Further,Chyawanprash significantly suppressed epidermal thickening,a typical marker of skin photoaging,in mice.We then analyzed the effect of Chyawanprash in human cells in culture,and found that Chyawanprash enhanced the growth of human keratinocytes,and efficiently eliminated ROS,which are causally involved in skin photoaging,in HeLa cells.CONCLUSION:These findings suggested that Chyawanprash may have beneficial effects on slowing skin photoaging.展开更多
基金supported by Zhejiang Provincial Natural Science Foundation of China(grant no.LQ22H150005)。
文摘Background:Skin aging has recently gained significant attention in both society and skin care research.Understanding the biological processes of photoaging caused by long-term skin exposure to ultraviolet radiation is critical for preventing and treating skin aging.Therefore,it is important to identify genes related to skin photoaging and shed light on their functions.Methods:We used data from the Gene Expression Omnibus(GEO)database and conducted bioinformatics analyses to screen and extract microRNAs(miRNAs)and their downstream target genes related to skin photoaging,and to determine possible biological mechanisms of skin photoaging.Results:A total of 34 differentially expressed miRNAs and their downstream target genes potentially related to the biological process of skin photoaging were identified.Gene Ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that these target genes were enriched in pathways related to human papillomavirus infection,extracellular matrix(ECM)-receptor signaling,estrogen receptor,skin development,epidermal development,epidermal cell differentiation,keratinocyte differentiation,structural components of the ECM,structural components of the skin epidermis,and others.Conclusion:Based on the GEO database-derived findings,we determined that target genes of two miRNAs,namely miR-4667-5P-KRT79 and miR-139-5P-FOS,play an important role in skin photoaging.These observations could provide theoretical support and guidance for further research on skin aging-related biological processes.
基金funding from the Discipline construction project of Guangdong Medical University(4SG21277P,2021ZDZX2039,2DK21003,4SG23060G)Our work described in the present manuscript was also supported by research grants from Guangdong Provincial Natural Science Foundation(2021A1515012437,2021KTSCX040),Guangdong,China。
文摘Skin photoaging is induced and sustained by UV-induced oxidative damage,and stimulating regeneration of the UV-induced aging has remained a great challenge due to high-level oxidative stress factor(ROS)-induced chronic oxidative damage and inactivation of bio-macromolecule-based regeneration in oxidative photoaging micro-environment.In this study,we designed a“seed and soil”strategy to pursue a safer and more efficient way to prevent and treat photoaging by simultaneously changing UV-induced ROS-rich micro-environment into a proregenerative one(the“soil”)and providing growth factor-rich platelet lysates(PL,the“seed”)using PL-impregnated,collagen-reinforce hydrogel(PL/Col).SD rats were used to establish photoaging model by 8 weeks of UV irradiation.The effectiveness of different treatments was evaluated by making pathological sections and detecting photoaging-related indicators.Rats treated with PL/Col demonstrated a significant acceleration in skin healing and enhancement in the quality of trauma repairing.After treated with PL/Col,the rats showed smooth yellowish appearance,integral structure of skin collagen fiber and epidermis,a decrease in inflammation and a reshaped active micro-environment with reduced levels of SOD enzyme activity,GSH enzyme activity and MDA toxic products.Treatment of PL/Col in skin photoaging has shown potential anti-oxidation and anti-aging effects and is worthy of further study in related field.
文摘Background and Objectives:Microneedling has been introduced as a new technique to address the growing concern of facial skin photoaging.Kangfuxin liquid has been found to promote the process of skin wound repair,including reducing inflammatory response,improving immunity and enhancing antioxidant levels.In this prospective randomized double-blinded study,we wanted to explore the clinical efficacy and safety of Kangfuxin liquid combined with microneedling in treating facial skin photoaging.Methods:57 patients with facial skin photoaging were randomly divided into two groups.The treatment group(28 cases)received microneedle therapy with Kangfuxin liquid,while the control group(29 cases)received microneedle therapy with physiological saline.The treatment interval was 4 weeks,and a total of 3 treatments were performed.Compare the VISIA scores of facial photoaging features such as wrinkles,texture,pores,spots and ultraviolet pigmentation between two groups of patients before and after treatment,Global Score for Photoaging,satisfaction evaluation,and record the occurrence of adverse reactions.Results:After treatment,the treatment group showed more significant improvement in wrinkles,texture,pores,spots and ultraviolet pigmentation,and the Global Score for Photoaging was better than the control group(P<0.05).The satisfaction rate with improving skin in the treatment group was 85.71%,which was higher than 75.86%in the control group(P<0.05).Both groups did not experience adverse reactions such as skin infection,pigmentation or hypopigmentation,scar formation,or worsening of melasma.Conclusion:Kangfuxin liquid combined with microneedling therapy has a good improvement effect on facial skin photoaging,with a low incidence of adverse reactions and high patient satisfaction.It is worthy of clinical promotion.
基金supported by the National Natural Science Foundation of China (No.31401476)Specialized Research Fund for the Doctoral Program of Higher Education (No.20130132120024)+1 种基金the Fundamental Research Funds for The Central Universities (No.201313002)Shandong Province Regional Innovation and Development of Marine Economy Demonstration Projects
文摘The objective of this study was to investigate the effect of gelatin(SG) isolated from salmon skin and its hydrolysate(SGH) on photoaging skin, and the mechanism responsible for anti-photoaging. The average molecular weights of SG and SGH were 65 k Da and 873 Da, respectively. The amino acid compositions of SG and SGH were similar. Both of them were abundant in hydrophobic amino acids. Twenty-five peptides were identified from SGH. SG and SGH could improve UV irradiation-induced pathological changes of macroscopical tissue texture and skin morphology. Hydroxyproline content is an indicator of matrix collagen content, SG and SGH could inhibit the decrease of hydroxyproline content in photoaging skin in a dose dependent manner. In addition, SG and SGH could alleviate UV irradiation-induced oxidative damages to skin by increasing the activities of total superoxide dismutase(T-SOD), glutathione peroxidase(GSH-Px) and catalase(CAT), increasing the content of glutathione(GSH) and decreasing the content of malonaldehyde(MDA). Moreover, SG and SGH could enhance immune regulation system by increasing the thymus index. Thus, the anti-photoaging mechanisms of SG and SGH were by inhibiting the depletion of antioxidant defense components, involving in the synthesis of collagen and enhancing the function of immune system. Besides, SGH showed a better result in protecting skin from photoaging than SG.
基金supported by Biological Resources Programme,Chinese Academy of Sciences (Grant No.KFJ-BRP-007-019)National Natural Science Foundation of China (No.32170407) and Beijing DR PLANT Biotechnology Co.,Ltd.
文摘Dendrobium officinale is a valuable medicinal herb that is widely used in traditional Chinese medicine.The chemical constituents of D.officinale have attracted much attention and a large number of compounds have been reported including many bibenzyl derivatives.13 bibenzyl derivatives from D.officinale were sent for molecular docking,sur-face plasmon resonance(SPR)assay and after detection of Mn-SOD and SIRT3 activities in or not in HaCaT cells,it was concluded that bibenzyl derivatives did not directly activate Mn-SOD but promoted SIRT3 proteins.In addition,HaCaT cells were irradiated with UV-B to induce an oxidative stress model in vitro to further verify the effect of bibenzyl derivatives.The results show that bibenzyl derivatives could directly bind to SIRT3,enhance the deacetylation and then activate Mn-SOD,so as to protect UV-B induced skin photoaging.
基金supported by Yunnan Provincial Department of Education Science Research Fund Project(2021Y426/2018JS411).
文摘Skin is the largest organ of human body and it protects the organism from external stimuli.Skin will age as the organism ages in normal circumstances.Skin photoaging refers to the accumulation of ultraviolet radiation due to long-term exposure to sunlight,which results in the premature aging of skin,wrinkles,pigmentation,skin laxity,and other signs of aging.The increase in the level of reactive oxygen species(ROS)induced by ultraviolet radiation,which causes the oxidation of cells,proteins,lipids,and other components,is an important cause of accelerated photoaging of skin.For this reason,the skin's antioxidant system will further play an antioxidant role to reduce oxidative damage by inhibiting ROS production,breaking down ROS,and degrading oxides.Therefore,it is important to understand the mechanism of skin damage caused by Ultraviolet radiation,the effects of reactive oxygen species and how the body's antioxidant system exerts its antioxidant effect.This will not only deepen the understanding of skin photoaging but also provide a scientific basis for the research on how to prevent and treat photoaging.
文摘The skin is a major protective organ of the body. It is constantly exposed to the environment and is very resilient. But exposure to ultraviolet (UV) rays from the sun results in the production of reactive oxygen species (ROS) and subsequent inflammatory responses that can overwhelm the innate protective mechanisms of the skin. This results in damage and premature aging. Strategies to mitigate this premature photoaging might include avoidance of sunlight. However, some sunlight exposure is beneficial to health. One notable example of this is the production of vitamin D. A more practical approach to preventing adverse effects of UV light in the skin is antioxidant supplementation. Dietary antioxidants may help control ROS propagation following UV light exposure. To further evaluate the utility of antioxidants in protecting the skin, in vitro, in vivo and human studies of three well known dietary antioxidants are reviewed and discussed. The data clearly demonstrate that vitamin C, grape seed extract and citrus bioflavonoids have the potential to reduce the damaging effects of excess sun exposure via antioxidant, anti-inflammatory and immunomodulating mechanisms. As such, regular ingestion of dietary antioxidants appears to be a useful strategy for protecting the skin against photoaging.
文摘Physiological processes, as aerobic metabolism and inflammatory response, generate reactive oxygen species (ROS) that may induce cellular injury when their amount is increased and antioxidant defense mechanisms are overwhelmed. Also, ROS are generated following UV skin irradiation able to deplete the natural antioxidant defenses in the skin. The increase in exposure to UV may lead to photoaging and precancerous skin lesions (actinic keratosis). New antioxidant strategies in the prevention and therapy of skin lesions are urgently needed. In this study, we evaluated the antioxidant efficacy of a recombinant form of human manganese superoxide dismutase able to inhibit reactive oxygen species production in some patients affected by severe photoaging and actinic keratosis.
文摘Photoaging is an accelerating aging process of the skin due to prolonged exposure to UV from the Sun or other sources. Herbal extracts, natural compounds, and bioactive polypeptides have widely used in cosmetic agents for protection of the skin against photoaging. This mini review briefly summarizes topical use of selected most common medicinal herbs, naturopathic chemicals, and bioactive peptides examined for skin protection.
文摘Skin photoaging is a complex, multifactorial process resulting in functional and structural changes of the skin, and different phenotypes from chronological skin aging are well-recognized. Ultraviolet (UV)-irradiated hairless mice have been used as a skin photoaging animal model. However, differences in morphology and gene expression patterns between UV-induced and chronological skin changes in this mouse model have not been fully elucidated. Here we investigated differences in histopathology and cytokine expression between UV-irradiated and non-irradiated aged hairless mice to clarify the factor(s) that differentiate photoaging from chronological skin aging phenotypes. Eight-week-old HR-1 hairless mice were divided into UV-irradiated (UV-irradiated mice) and non-irradiated (control mice) groups. Irradiation was performed three times per week for 10 weeks. In addition, 30-week-old HR-1 hairless mice were reared until 70 weeks of age without UV irradiation (aged mice). Histopathologies revealed that the flattening of dermal-epidermal junctions and epidermal thickening were observed only in UV-irradiated mice. Decreases in fine elastic fibers just beneath the epidermis, the thickening of elastic fibers in the reticular dermis, and the accumulation of glycosaminoglycans were more prominent in UV-irradiated mice as compared to non-irradiated aged mice. Quantitative PCR analyses revealed that UV-irradiated mice showed an increase in the expression of IFN-γ. In contrast, aged mice exhibited proportional up-regulation of both pro-inflammatory and anti-inflammatory cytokines. The IFN-γ/IL-4 ratio, an indicator for the balance of pro-inflammatory and anti-inflammatory cytokines, was significantly higher in UV-irradiated mice as compared to control and non-irradiated aged mice. An elevated IFN-γ/IL-4 ratio was also observed in aged senescence-accelerated mouse-prone 1 (SAMP1) mice, a spontaneous skin photoaging model we recently reported. Thus, an imbalance between pro-inflammatory and anti-inflammatory cytokines might be a key factor to differentiate photoaged skin from chronologically-aged skin.
基金supported by the Fund for National Key R&D Program of China(No.2021YFC3200401)the National Natural Science Foundation of China(Nos.52170024,21677015,22006031).
文摘Plastic products widespread in natural water can be broken into smaller-sized microplastics(MPs,<5 mm)under light irradiation,thermal degradation and biodegradation,posing a serious threat to aquatic ecosystems and human health.This perspective concludes that MPs can generate reactive oxygen species(ROS)through initiation,propagation and termination steps,which can attack the polymer resulting in the photoaging and breakdown of C–C and C–H bonds under ultraviolet(UV)irradiation.Free radical generation and weathering degree of MPs depend on their physicochemical properties and environmental conditions.In general,UV irradiation and co-existed MPs can significantly accelerate MP photoaging.With plentiful chromophores carbonyl,carboxyl and benzene rings,Dissolved organic matter(DOM)mainly absorbs photons(300–500 nm)and generates hydrated electrons,^(3)DOM^(*) and ROS,which may affect MP photoaging.However,whether DOM may transfer the electron and energy to MPs under UV irradiation,affect ROS generation of MPs and their photoaging pathway are inadequately studied.More studies are needed to elucidate MP photoaging pathways and mechanisms,consider the influence of stabilization capacity,photosensitization and photoionization of DOM as well as their competitive light absorption with MPs,which provides valuable insights into the environmental behavior and ecological risk of MPs in natural water.
基金supported by Shandong Provincial Natural Science Foundation (ZR2021QC118)Beijing Natural Science Foundation (7222249)the 2115 Talent Development Program of China Agricultural University。
文摘Objective: To evaluate whether α-ionone, an aromatic compound mainly found in raspberries, carrots,roasted almonds, fruits, and herbs, inhibits UVB-mediated photoaging and barrier dysfunction in a human epidermal keratinocyte cell line(HaCaT cells).Methods: The anti-photoaging effect of α-ionone was evaluated by detecting the expression of barrierrelated genes and matrix metalloproteinases(MMPs) in HaCaT cells. The levels of reactive oxygen species,oxidation product, antioxidant enzyme, and inflammatory factors were further analysed to underline the protective effect of α-ionone on epidermal photoaging.Results: It was found that α-ionone attenuated UVB-induced barrier dysfunction by reversing keratin 1and filaggrin in HaCaT cells. α-Ionone also reduced the protein amount of MMP-1 and mRNA expression of MMP-1 and MMP-3 in UVB-irradiated HaCaT cells, implying protective effects on extracellular matrix.Furthermore, HaCaT cells exposed to α-ionone showed significant decreases in interleukin(IL)-1β, IL-6,IL-8, and tumor necrosis factor-α as compared to UVB-irradiated HaCaT cells. α-Ionone treatment significantly inhibited the UVB-induced intracellular reactive oxygen species increase and malondialdehyde accumulation. Therefore, the beneficial effects of α-ionone on inhibiting MMPs secretion and barrier damage may be related to attenuated inflammation and oxidative stress.Conclusion: Our results highlight the protective effects of α-ionone on epidermal photoaging and promote its clinic application as a potential natural anti-photodamage agent in future.
文摘背景组织蛋白酶 B 在房间周期，细胞外的矩阵变化和皮肤的 tumorigenesis 起一个重要作用：它是否在未知的 photoaged 皮肤遗体起一个作用。这研究试图在 vivo 并且在 vitro 在皮肤 photoaging 调查组织蛋白酶 B 的角色。组织蛋白酶 B 的表情与在太阳的暴露的皮肤和六个健康中国志愿者的太阳的保护的皮肤的 immunohistochemical 方法相比的方法。在紫外轻 A (UVA ) 的组织蛋白酶 B 的 mRNA 和蛋白质表示在 vitro 导致了早熟的老朽成纤维细胞被即时反向的抄写聚合酶链反应(RT-PCR ) 和西方的弄污技术检测。结果减少了组织蛋白酶 B 的表示与太阳的保护的皮肤的相比在 photoaged 皮肤被观察。在 UVA 导致的、早熟的老朽成纤维细胞，组织蛋白酶 B 的更低的表情被西方的弄污检测，在一样的房间的组织蛋白酶 B mRNA 的减少的合成被即时 RT-PCR 揭示。结果在 vitro 并且在 vivo 表明了在皮肤 photoaging 和组织蛋白酶 B 之间的重要否定关联的结论。我们建议除矩阵 metalloproteinases 和抗氧化剂酶以外，组织蛋白酶 B 涉及皮肤 photoaging 的过程因为它贡献细胞外的矩阵改变并且是在细胞的 apoptosis 和老朽的主导的朊酶。
基金supported in part by a grant-in-aid for Scientific Research from the Ministry of Education,Science and Culture of Japan
文摘OBJECTIVE:Chronic exposure to ultraviolet(UV) radiation induces skin photoaging(premature skin aging).UV irradiation generates reactive oxygen species(ROS),which are shown to play a pivotal role in skin photoaging.Ayurveda is a holistic traditional medical system,and Chyawanprash is one of the most popular formulations in Ayurveda.Since maintenance of the function and appearance of skin is important,we examined whether Chyawanprash has a protective effect on skin photoaging.METHODS:To examine the effect of Chyawanprash on skin photoaging,hairless mice were administered with Chyawanprash in drinking water for 3 weeks,and then repeatedly exposed to ultraviolet light B(UVB) irradiation(225 or 450 mJ/cm2) to induce skin photoaging.To further examine the function of Chyawanprash,its effects were examined in cells cultured In vitro.Chyawanprash was added in culture medium,and examined for the effect on the growth of human keratinocytes,and for the ability to eliminate ROS which generated by paraquat(50 pmol/L) in HeLa cells.RESULTS:UVB irradiation caused symptoms such as rough skin,erythema,and edema on the skin in hairless mice,but administration of Chyawanprash relieved these symptoms.Further,Chyawanprash significantly suppressed epidermal thickening,a typical marker of skin photoaging,in mice.We then analyzed the effect of Chyawanprash in human cells in culture,and found that Chyawanprash enhanced the growth of human keratinocytes,and efficiently eliminated ROS,which are causally involved in skin photoaging,in HeLa cells.CONCLUSION:These findings suggested that Chyawanprash may have beneficial effects on slowing skin photoaging.
