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不同生长基质中叶状地衣共生藻的分离与鉴定 被引量:5
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作者 张青 史全良 《生态环境学报》 CSCD 北大核心 2010年第12期2850-2856,共7页
采用形态鉴定及基于ITS及rbcL序列构建ML系统发育树对采自云南省姚安县及南华县的10种叶状地衣中分离纯化培养的共生藻进行分析研究。鉴定结果表明5个编号分别为Z1、Z4、Z5、Z6、Z9的树生地衣共生藻样品鉴定为念珠藻目(Nostocales)念珠... 采用形态鉴定及基于ITS及rbcL序列构建ML系统发育树对采自云南省姚安县及南华县的10种叶状地衣中分离纯化培养的共生藻进行分析研究。鉴定结果表明5个编号分别为Z1、Z4、Z5、Z6、Z9的树生地衣共生藻样品鉴定为念珠藻目(Nostocales)念珠藻科(Nostocaceae)念珠藻属(Nostoc);2个土生地衣共生藻样品Z2、Z10鉴定为四孢藻目(Tetrasporales)胶球藻科(Coccomyxaceae)胶球藻属(Coccomyxa);3个石生地衣分离藻样品Z3、Z7、Z8鉴定为绿球藻目(Chlorococcales)栅藻科(Scenedesmaceae)链带藻属(Desmodesmus)。另外从地衣采集地及分子系统分析结果可以看出,尽管Z1、Z5、Z9分别是从采自不同区域(姚安县及南华县)的地衣中分离纯化的,但亲缘关系最近;同样Z4、Z6亲缘关系最近;Z2、Z10亲缘关系最近,Z7、Z8亲缘关系最近。所以地衣共生菌与共生藻的共生关系与其生存的地理区域关系不大,但却和其生长基质有明显的相关性。 展开更多
关键词 地衣 共生藻 生长基质 ITS序列分析 系统发育 鉴定
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Diversity of lichen flora of Odisha,India-A review
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作者 Nayak SK Bajpai R +1 位作者 Upreti DK Satapathy KB 《Studies in Fungi》 2016年第1期114-124,共11页
Based on the compilation of literature on lichens of Odisha(India)and other specimens and preserved in herbaria,we estimate that 252 species of lichens,belonging to 81 genera and 35 families are known from the state.G... Based on the compilation of literature on lichens of Odisha(India)and other specimens and preserved in herbaria,we estimate that 252 species of lichens,belonging to 81 genera and 35 families are known from the state.Graphidaceae with 49 species is the dominant family,followed by Arthoniaceae(21),Pyrenulaceae(19),Parmeliaceae(18),Trypetheliaceae(17),Physciaceae(15),Teloschistaceae(14),Lecanoraceae(13),Pertusariaceae(12),Ramalinaceae(11),Caliciaceae(11),Thelotremataceae(9)and Lecidiaceae(4).Among the lichen genera Graphis is dominant with 27 species,followed by Pyrenula(16),Caloplaca(14),Pertusaria(12),Lecanora(10),Parmotrema(9),Cryptothecia(9)and Pyxine(6).Accounts of lichens are only available from 13 out of 30 districts.Of these only cursory collections from 10 districts are available,whereas Mayurbhanj,Jharsuguda and Ganjam districts are well-explored for their lichen flora.The present enumeration of more than 250 species clearly indicates a rich diversity of lichens for the state.Further exploration in other unexplored districts will add more species to the lichen flora of the state. 展开更多
关键词 Ganjam Jharsuguda Mayurbhanj MYCOBIONT phycobiont
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Survival analyses of symbionts isolated from Endocarpon pusillum Hedwig to desiccation and starvation stress 被引量:13
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作者 ZHANG Tao WEI JiangChun 《Science China(Life Sciences)》 SCIE CAS 2011年第5期480-489,共10页
This work deals with the survival analyses of the symbionts isolated from the lichen E.pusillum under desiccation and starvation stress.The mycobiont of the symbionts was under the desiccation in combination with star... This work deals with the survival analyses of the symbionts isolated from the lichen E.pusillum under desiccation and starvation stress.The mycobiont of the symbionts was under the desiccation in combination with starvation stress,and under starvation stress alone as well.The phycobiont of the symbionts was under desiccation stress alone.The experiments were detected by means of the biomass size,weight and cell density,deformity of the hyphae and cells,and metabolic activity through SEM (scanning electron microscopy),TEM (transmission electron microscopy),FM (fluorescence microscopy),spectrophotometry,and FCM (flow cytometry).The results show that the mycobiont can survive for seven months under desiccation stress in combination with starvation stress,and for eight months under starvation stress alone.The phycobiont can survive for two months under desiccation stress.It can provide a scientific basis for further research of the reproduction biology of lichens and arid desert biocarpet engineering to fix sand and carbon. 展开更多
关键词 LICHENS MYCOBIONT phycobiont SEM TEM FM FCM REPRODUCTION biocarpet
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石果衣菌藻次级代谢产物的初步研究 被引量:5
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作者 柳润东 蒋淑华 +1 位作者 李二伟 魏江春 《菌物学报》 CAS CSCD 北大核心 2018年第2期246-255,共10页
荒漠地衣石果衣Endocarpon pusillum中未能检测出任何次级代谢产物,然而,其共生菌基因组中却含有14个PKS基因和2个NRPS基因。通过激活培养后从中分离出3个次级代谢产物,4,6,9-三羟基-7-甲基-1H,3H-萘并[1,8-cd]吡喃-1,3-二酮(lamellicol... 荒漠地衣石果衣Endocarpon pusillum中未能检测出任何次级代谢产物,然而,其共生菌基因组中却含有14个PKS基因和2个NRPS基因。通过激活培养后从中分离出3个次级代谢产物,4,6,9-三羟基-7-甲基-1H,3H-萘并[1,8-cd]吡喃-1,3-二酮(lamellicolic anhydride)、石果衣菌素A(endocarpin A)及石果衣菌素B(endocarpin B),后二者为新结构化合物。从其共生藻柯氏复球藻Diplosphaera chodatii中分离出3个脱镁叶绿素类化合物,即脱镁叶绿素甲酯酸b(phaeophorbide b)、13-表-脱镁叶绿素甲酯酸a(13-epi-phaeophorbide a)及脱镁叶绿素甲酯酸a(phaeophorbide a)。其中脱镁叶绿素甲酯酸b具有中等的清除DPPH活性。 展开更多
关键词 地衣型真菌 地衣共生藻 柯氏复球藻 异吲哚-1-酮类 萘醌类
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