[Objective]This study aimed to isolate and identify the insecticidal compound produced by the Streptomyces strain.[Method]Extraction,PTLC,HPLC,and other instrumental analysis were applied to obtain the bioactive compo...[Objective]This study aimed to isolate and identify the insecticidal compound produced by the Streptomyces strain.[Method]Extraction,PTLC,HPLC,and other instrumental analysis were applied to obtain the bioactive compound.[Result]Yellow-white crystals were obtained and their MS and NMR spectra were analyzed.[Conclusion]Crystals were identified as the analogue of dimeric dinactin.展开更多
Phytochemical investigation of the purified fractions of the active dichloromethane extract of Hysterionica pinifolia (Poir.) Baker led to the identification of five compounds. New acetylenic alcohol (E)-undec-3-en-5,...Phytochemical investigation of the purified fractions of the active dichloromethane extract of Hysterionica pinifolia (Poir.) Baker led to the identification of five compounds. New acetylenic alcohol (E)-undec-3-en-5,7-diyne-1-ol (1) and three other compounds (3), (4), and (5) were reported for the first time in this species. Furthermore, forty-six components from the volatile fraction of H. pinifolia were identified. These compounds were elucidated using 1D and 2D NMR spectroscopy as well as MS-ESI and GC-FID-MS experiments. The dichloromethane extract, its fractions, and the methanolic extract were tested for insecticidal activity against Tribolium castaneum under laboratory conditions. The dichloromethane extract and the fraction F2 were found to be active, showing high larval mortality. The dichloromethane extract was also active against T. castaneum adults. The results have shown that H. pinifolia could be considered, in a near future, as a potential source for the development of a botanical insecticide for pest control.展开更多
Nicotine and nornicotine-typed compounds are well-known for its potential as insecticide.Some researches have shown that nicotine was highly toxic to Diaphorina citri Kuwayama,an invasive and fast spreading pest.Here ...Nicotine and nornicotine-typed compounds are well-known for its potential as insecticide.Some researches have shown that nicotine was highly toxic to Diaphorina citri Kuwayama,an invasive and fast spreading pest.Here we report the effects of nornicotine-typed insecticide against D.citri.Novel compounds A1-A8,B1-B28,C1-C18 and D1-D12 were synthesized by reported procedure.And bioassays indicated that some of these compounds exhibited good effects against D.citri.Compounds B2,B3,B8,B12,B13,B22,C5,C7,D5,and D9 exhibited the mortality up to 100%within 120 h in 50 mg/L.And further data shows that A2-5,A7,B1,B8,B20 and B22 showed rapid insecticidal activities against D.citri in 24 h with the low LC_(50)(LC_(50)<50 mg/L in 24 h,and LC_(50)<30 mg/L in 72 h).Nornicotine-typed derivatives exhibited activity against D.citri,and B8 and B22 exhibited the best insecticidal activity with an LC_(50)of 18.01.0,18.20.9 mg/L in 24 h,which had the lower cost than nicotine and showed the potential as powerful insecticides.展开更多
基金Supported by Project of Science and Technology Research for Colleges and Universities in Hebei Province(Z 2011151)National Natural Science Foundation of China(No.30970010)Key Discipline of Bioengineering in Hebei Province
文摘[Objective]This study aimed to isolate and identify the insecticidal compound produced by the Streptomyces strain.[Method]Extraction,PTLC,HPLC,and other instrumental analysis were applied to obtain the bioactive compound.[Result]Yellow-white crystals were obtained and their MS and NMR spectra were analyzed.[Conclusion]Crystals were identified as the analogue of dimeric dinactin.
文摘Phytochemical investigation of the purified fractions of the active dichloromethane extract of Hysterionica pinifolia (Poir.) Baker led to the identification of five compounds. New acetylenic alcohol (E)-undec-3-en-5,7-diyne-1-ol (1) and three other compounds (3), (4), and (5) were reported for the first time in this species. Furthermore, forty-six components from the volatile fraction of H. pinifolia were identified. These compounds were elucidated using 1D and 2D NMR spectroscopy as well as MS-ESI and GC-FID-MS experiments. The dichloromethane extract, its fractions, and the methanolic extract were tested for insecticidal activity against Tribolium castaneum under laboratory conditions. The dichloromethane extract and the fraction F2 were found to be active, showing high larval mortality. The dichloromethane extract was also active against T. castaneum adults. The results have shown that H. pinifolia could be considered, in a near future, as a potential source for the development of a botanical insecticide for pest control.
基金the financial support from the National Natural Science Foundation of China(31901786,22078263)the National Key R&D Program of China(2021YFC2101500)+2 种基金the National Key R&D Program of China(2021SF-116,2023-YBSF-396)the Natural Science Foundation of Shaanxi Province(2022JQ-112)the China Postdoctoral Science Foundation(2021M692610).
文摘Nicotine and nornicotine-typed compounds are well-known for its potential as insecticide.Some researches have shown that nicotine was highly toxic to Diaphorina citri Kuwayama,an invasive and fast spreading pest.Here we report the effects of nornicotine-typed insecticide against D.citri.Novel compounds A1-A8,B1-B28,C1-C18 and D1-D12 were synthesized by reported procedure.And bioassays indicated that some of these compounds exhibited good effects against D.citri.Compounds B2,B3,B8,B12,B13,B22,C5,C7,D5,and D9 exhibited the mortality up to 100%within 120 h in 50 mg/L.And further data shows that A2-5,A7,B1,B8,B20 and B22 showed rapid insecticidal activities against D.citri in 24 h with the low LC_(50)(LC_(50)<50 mg/L in 24 h,and LC_(50)<30 mg/L in 72 h).Nornicotine-typed derivatives exhibited activity against D.citri,and B8 and B22 exhibited the best insecticidal activity with an LC_(50)of 18.01.0,18.20.9 mg/L in 24 h,which had the lower cost than nicotine and showed the potential as powerful insecticides.
