The effects of different treatments on the seedlessness and fruit quality of‘Miguang’table grape was studied by using plant growth regulators,gibberellin acid(GA 3)and forchlorfenuron(CPPU),under different concentra...The effects of different treatments on the seedlessness and fruit quality of‘Miguang’table grape was studied by using plant growth regulators,gibberellin acid(GA 3)and forchlorfenuron(CPPU),under different concentrations and application time.The results showed that the effects of different treatments on the seedlessness and fruit quality were different.Seedless rate,cluster weight,berry weight,berry shape index,soluble solid content,total acid content,soluble solids to acidity ratio,pulling resistance,turgor pressure and flesh firmness without skin were comprehensively evaluated,as a result of which,the optimum treatment on‘Miguang’table grape was to apply with GA 320 mg/L+SM 200 mg/L at one week before bloom and GA 325 mg/L+CPPU 3 mg/L at two weeks after bloom.展开更多
Global concerns about the environmental impact of combustion emissions from petroleum fuels influence new research to seek for alternative energy sources. The current study investigates the possibility of using safflo...Global concerns about the environmental impact of combustion emissions from petroleum fuels influence new research to seek for alternative energy sources. The current study investigates the possibility of using safflower (Carthamus tinctorius L.) as an alternative biodiesel raw material. Four plant growth regulators (PGR) were used to boost the production of safflower. Thirteen treatments were constituted from the four plant regulators and applied to the safflower crop arranged in completely randomised design, repeated three times. The results show that the effect of plant growth regulators was not more than that of the control. More studies have to be channelled towards the relationship between safflower and plant growth regulators.展开更多
[Objective] This study aimed to investigate the influence of different plant growth regulators on apricot pollen germination and pollen tube growth. [Method] Pollens of six kinds of Xinjiang apricots were cultured in ...[Objective] This study aimed to investigate the influence of different plant growth regulators on apricot pollen germination and pollen tube growth. [Method] Pollens of six kinds of Xinjiang apricots were cultured in solid media supplemented with five plant growth regulators （GA3 , NAA, 2, 4-D, 6-BA, IAA）. Then the rate of pollen germination and the length of pollen tube were respectively measured. [Result] In a certain concentration range, GA3 most significantly promoted the pollen germination and the pollen tube growth of Shushanggan, Kalayulvke, Dayoujia, Yiliakeyulvke and Kabakehuanna; NNA had the strongest improvement function on Kumaiti’s pollen germination and pollen tube growth. [Conclusion] All the five plant growth regulators promoted the pollen germination and the pollen tube growth of apricots at low concentration but inhibited them at high concentration.展开更多
The effects of four kinds of plant growth regulators with different concentrations on narcissi were studied in 2001. The results showed that the regulators could inhibit the growths of height and leaves of narcissi. O...The effects of four kinds of plant growth regulators with different concentrations on narcissi were studied in 2001. The results showed that the regulators could inhibit the growths of height and leaves of narcissi. Of the four regulators, the dwarfing effects of paclobatrazol (PP333) and uniconazole (S3307) on narcissi were better than those of chlorocholine (CCC) and dimethyl amino-sussinamic acid (B9). All of the regulators did not have significant effect on the root length. Moreover, the time of flowering was later for the narcissi treated with regulators than that of the control to a certain extent, and the range delayed was from 2 days to 19 days. The correlation analysis results showed that there was a significant correlation between the time of flowering and the concentrations of regulators. The ornament value of narcissi was obviously improved by using the regulators.展开更多
[Objective] This study was to investigate the effects of plant growth regulator on accumulation and circulation of potassium in flue-cured tobacco.[Methods] Hydroponics experiment was adopted to study the effects of p...[Objective] This study was to investigate the effects of plant growth regulator on accumulation and circulation of potassium in flue-cured tobacco.[Methods] Hydroponics experiment was adopted to study the effects of plant growth regulator（NAA,GA3,BR） on accumulation and circulation of potassium in flue-cured tobacco.[Results] The BR treatment increased the absorption of potassium,calcium and magnesium in flue-cured tobacco,reduced the potassium emission from the root,enhanced the accumulation and contents and promoted the circulation of potassium in the tobacco plants.There was no statistical difference between the NAA and GA3 treatment.And BR treatment was 0.52%,0.30%,0.28% higher than NAA treatment in enhancing potassium content in tobacco plants at 2,7,12 d after topping.In a word,the results showed that BR treatment was the most.[Conclusion] BR treatment could effectively enhance potassium content in tobacco plants after topping.展开更多
[Objective]In order to increase anther culture efficiency of pepper.[Method]MS culture media and Bolajiaohong were used in this experiment to study the influences of carbon sources and concentrations on anther callus ...[Objective]In order to increase anther culture efficiency of pepper.[Method]MS culture media and Bolajiaohong were used in this experiment to study the influences of carbon sources and concentrations on anther callus induction of pepper.Jiayu was taken as a material to study influences of plant growth regulators and concentrations on anther callus induction of pepper according to L16（4^5） orthogonal design.[Result]The average callus and embryoid induction rates of maltose at all concentrations were higher than these of sucrose but the difference was not significant.Taking maltose or sucrose as a carbon source,3% to 6% concentration was good for increasing induction frequencies of calli and embryoids.However,If the concentration was over 6%,the induction rates were declined dramatically with the increase of sugar concentration.The influences of growth regulators on induction rate of calli were listed as 2,4-D﹥ZT﹥NAA﹥KT﹥6-BA;the influences on induction rates of embryoids were listed as 2,4-D﹥NAA﹥ZT﹥KT﹥6-BA.The 2,4-D,ZT,NAA and KT had signficant or extremely significant influences on induction rates of calli and embryoids.2,4-D,ZT at 1.0 mg/L and NNA,KT at 0.5 mg/L had the best effects.The influences of ZT on calli and embryoids were better than those of KT and 6-BA.1.0 mg/L 2,4-D ＋1.0 mg/L ZT ＋0.5 mg/L KT ＋0.5 mg/L 6-BA was the best regulator combination for induction culture of Jiayu anther.[Conclusion]The experiment provided research basis for anther culture of pepper.展开更多
The effect of vernalization and two growth regulators Fascination^TM and Pro-Gibb^R on the growth, inflorescence development and flowering of Omithogalurrt Chesapeake Snowflake＇ was studied. Regardless of growth regu...The effect of vernalization and two growth regulators Fascination^TM and Pro-Gibb^R on the growth, inflorescence development and flowering of Omithogalurrt Chesapeake Snowflake＇ was studied. Regardless of growth regulator treatment, chilling bulbs for 3 weeks at 10℃ before planting accelerated flowering of the first inflorescence by 5 to 6 days, elongated floral stem length by 1.5 to 2 cm and shortened leaf length by 4 to 5 cm as compared with non-chilled bulbs. When bulbs were chilled 3 weeks at 10℃ before planting, Fascination 2% and Pro-Gibb accelerated flowering of the first inflorescence by 2 to 6 days respectively as compared with the control. When bulbs were not chilled before planting, Fascination 2% and Pro-Gibb accelerated flowering of the first inflorescence by 6 to12 days respectively as compared with the control. The lengths of the leaves and the inflorescences were not affected by PGR treatment. Higher rates of Pro-Gibb （100 mg.L^-1 and 200 mg.L^-1） and more amount of Fascination 2%（100 μL and 200 μL） can cause abortion of inflorescence.展开更多
[Objective] The aim was to investigate the effects of plant growth regulators on contents of reducing sugar and starch in potato（Solanum tuberosum L.）.[Method]A potato cultivar named ＇Holand-212＇ with three treatm...[Objective] The aim was to investigate the effects of plant growth regulators on contents of reducing sugar and starch in potato（Solanum tuberosum L.）.[Method]A potato cultivar named ＇Holand-212＇ with three treatments by spraying Diethyl aminoethyl hexanoate（DTA-6）,Uniconazole（S3307）and SOD mimics（SODM）were employed to compare differences of the contents of reducing sugar and starch in potato leaves,stolons and tubers in a field experiment.[Result]DTA-6 treatment could significantly reduce the contents of starch and reducing sugar in potato stolons in the period of 100-110 d after seeding,and it could also reduce the content of reducing sugar in tubers,but there is an opposite result in leaves in the same period,significantly.S3307 could extremely significantly increase the content of starch,and can promote the transfer of starch in stolons,in addition,it also could reduce the content of reducing sugar in tubers,and extremely significantly increase the content of starch in leaves at the 110 d after sowing.Moreover,there is a similar effect between SODM and S3307.[Conclusion]The research results are significant for understanding mechanisms of plant growth regulators and promoting application of plant growth regulator in potato.展开更多
The endangered tropical tree, Aquilaria malaccensis, produces agarwood for use in fragrance and medicines. Efforts are currently un-derway to produce valuable agarwood compoundsn tissue culture. The purpose of this st...The endangered tropical tree, Aquilaria malaccensis, produces agarwood for use in fragrance and medicines. Efforts are currently un-derway to produce valuable agarwood compoundsn tissue culture. The purpose of this study was to develop an optimal growth medium, specif-ically, the best hormone combination for callus suspension culture. Using nursery-grown A. malaccensis, sterilized leaf explants were first incu-bated on basic Murashige and Skoog （MS） gel medium containing 15g/L sucrose and at pH 5.7. Different auxin types including 1-naphthaleneacetic acid （NAA）, 2,4-dichlorophenoxyacetic acid （2,4-D）, and indole-3-butyric acid （IBA）, were tested at various concentrations （0.55, 1.1 and 1.65 μM） using the basic medium. Leaf explants were incubated for 30 days in the dark. Callus induced by 1.1 μM NAA had the highest biomass dry weight （DW） of 17.3 mg;however the callus was of a compact type. This auxin concentration was then combined with either 6-benzylaminopurine （BAP） or kinetin at 0.55, 1.1, 2.2 or 3.3 μM to induce growth of friable callus. The 1.1μM NAA＋2.2μM BAP com-bination produced friable callus with the highest biomass （93.3mg DW）. When testing the different carbon sources and pHs, sucrose at 15g/L and pH at 5.7 yielded highest biomasses at 87.7mg and 83mg DW, respec-tively. Microscopic observations revealed the arrangement of the friable cells as loosely packed with relatively large cells, while for the compact callus, the cells were small and densely packed. We concluded that MS medium containing 15 g/L sucrose, 1.1 μM NAA ＋ 2.2 μM BAP hor-mone combination, and a pH of 5.7 was highly effective for inducing friable callus from leaf explants of A. malaccensis for the purpose of establishing cell suspension culture.展开更多
In this study,the seeds of wild Petunia Juss.were used as explants to investigate the optimal condition for tissue culture.Several different kinds and concentrations of growth regulators were adopted to produce more m...In this study,the seeds of wild Petunia Juss.were used as explants to investigate the optimal condition for tissue culture.Several different kinds and concentrations of growth regulators were adopted to produce more multiple bud clumps,callus or roots in this study.The experiments may provide experimental foundation for the rapid propagation technology and establishment of tissue culture system for wild Petunia Juss.展开更多
The title compound 1-（3-amino-[1,2,4]triazol-1-yl）-3,3-dimethyl-butan-2-one（3） was synthesized by Hofmann-alkylation reaction of 1-chloro-3,3-dimethyl-butan-2-one（1） and ~1H-[1,2,4]triazol-3-ylamine（2） with eq...The title compound 1-（3-amino-[1,2,4]triazol-1-yl）-3,3-dimethyl-butan-2-one（3） was synthesized by Hofmann-alkylation reaction of 1-chloro-3,3-dimethyl-butan-2-one（1） and ~1H-[1,2,4]triazol-3-ylamine（2） with equal amount of K_2CO_3 as acid acceptor. The structure of compound 3 was characterized by ~1H NMR, 13 C NMR, HRMS and single-crystal X-ray diffraction. The compound crystallizes in the monoclinic system, space group P21/n with a = 5.7227（8）, b = 27.924（4）, c = 6.2282（7） ？, β = 101.892（11）°, V = 973.9（2） ？~3, Z = 4, T = 180.00（10） K, μ（MoKα） = 0.087 mm^（-1）, Dc = 1.243 g/cm^3, 3832 reflections measured（3.648≤θ≤26.022°）, 1916 unique reflections（Rint = 0.0359, Rsigma = 0.0572） used in all calculations. The final R = 0.0557（I 〉 2σ（I）） and w R = 0.1276（all data）. Bioassay showed that 3 displayed excellent activity as plant growth regulator with inducing lateral root formation and enhancing primary root elongation at 0.27 mmol/L（50 ppm） in soybeen（He Feng-50）. Good water solubility was found with 50 mg in 1 m L of water. Therefore, application of 3 in agriculture is more environmentally friendly due to cosolvent-free condition, and results in improved abiotic-stress tolerance by affecting the root growth. And furthermore, it can be used as a precursor to investigate the function of regulating plant root growth.展开更多
A stereocontrolled construction of the side chain of brassinclide and homobrassinolide has been achieved via the tandem vicinal dialkylation of the pyranone moiety as a key step.
[Objectives]This study was conducted to effectively monitor PGR residues in bean sprouts to provide guarantee for the food safety of agricultural products.[Methods]A high-performance liquid chromatography-tandem mass ...[Objectives]This study was conducted to effectively monitor PGR residues in bean sprouts to provide guarantee for the food safety of agricultural products.[Methods]A high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for the determination of residues of 15 plant growth regulators(PGRs)in bean sprouts was established using bean sprouts as an experimental material.Samples were extracted with a solution containing 5%acetic acid-acetonitrile(1∶99,V/V),purified with anhydrous magnesium sulfate,and diluted with methanol solvent to constant volume.The solutions were filtered through 0.22μm filtering membrane and the target analytes were separated on a Phenomenex H18 column.The identification of each compound was established by retention time matching along with the accurate mass measurement of the precursor ions and their main fragment ions.The quantification was carried out using matrix-matched external standard method.[Results]The retention time of the 15 PGRs were found in the range from 5.8-11.7 min under the optimized conditions.The linear relation was good in the concentration range of 0.005-0.050μg/ml,and the correlation coefficients of the 15 PGRs were≥0.9990.The limits of detection were in the range of 0.03-0.92 g/kg,and the limits of quantification were in the range of 0.50-2.10μg/kg.The average recovery in the recovery test at 3 concentration levels was 80%-110%,and the relative standard deviations were in the range of 2.8%-7.5%.[Conclusions]This method is simple and accurate,and can quickly qualitatively and quantitatively analyze the residues of 15 PGRs in bean sprouts.The proposed procedure was simple,quick and accurate for the simultaneous determination of the 15 PGRs in bean sprout.展开更多
Tests of new natural non-polluting regulators of growth Verva （vegetative terpenoids from Abies sibirica） and Larixin （vegetative flavonoids from Larix） on growth, development and productivity of fibre flax are co...Tests of new natural non-polluting regulators of growth Verva （vegetative terpenoids from Abies sibirica） and Larixin （vegetative flavonoids from Larix） on growth, development and productivity of fibre flax are conducted. The level of phytohormones in vegetating plants （IAA, ABA, zeatine and zeatinriboside） is studied. Preparations stimulate germination of seeds and growth of plants. Field experiment fixed yield increase of flax straw and the seeds of flax-fibre with the use of growth regulators. Industrial experiment represents VERVA product effectiveness on yields increase of flax straw, seeds and flax fiber output.展开更多
Chonemorpha fragrans is an endangered medicinal woody climber,regarded among alternative plant sources of camptothecin.Camptothecin is a monoterpene indole anti-cancer alkaloid with annual trade value of over three bi...Chonemorpha fragrans is an endangered medicinal woody climber,regarded among alternative plant sources of camptothecin.Camptothecin is a monoterpene indole anti-cancer alkaloid with annual trade value of over three billion U.S.dollars in the recent,and is used in the production of its analog drugs approved for the chemotherapy of cancer of varied types.Effects of plant growth regulators,culture media strength and photoperi-odic duration on the micropropagation ef ciency of C.fragrans from nodal segment explants were studied on Murashige and Skoog(MS)medium amended with Thidiazuron(TDZ),Benzylaminopurine(BAP)or Kinetin(Kin).Thidiazuron was more ef cient over BAP and Kin when half basal MS medium was used over full or quarter strength.Results of carbon source experiment showed sucrose as the most effective over glucose,fructose,and maltose in the clonal production.Studies on the photope-riodic incubation duration showed 12 h as the best light period and sub or supra-optimal resulted in the production of abnormal and albino micro shoots.Experimental results on the evaluation of physiological,biochemical parameters showed the role of pigment molecules and antioxidant systems in the production of albino micro shoots.展开更多
Most plant growth regulators available are synthesized and tend to be dangerous if used inappropriately. Emistim CAS is a natural plant growth regulator, with less side-effect. The research performed an assessment on ...Most plant growth regulators available are synthesized and tend to be dangerous if used inappropriately. Emistim CAS is a natural plant growth regulator, with less side-effect. The research performed an assessment on Emistim CAS application in watermelon cultivation in a greenhouse. The results showed that 0.11% Emistim CAS increased reducing sugar by 15.49%, soluble solid by 13.7%, Vc content by 3.72%, titratable acid by 18.1%, and yield per hectare by 18.01%, so that watermelon quality and yield were both improved. Emistim CAS can be widely promoted in watermelon cultivation in future.展开更多
Using Xinshikui No. 5 as the experimental material, the effects of different concentrations of mepiquat chloride （100, 200, 300,400, 500 mg/L）, chlormequat chloride （800, 1200,1 600, 2 000, 2 400 rag/L） and paclob...Using Xinshikui No. 5 as the experimental material, the effects of different concentrations of mepiquat chloride （100, 200, 300,400, 500 mg/L）, chlormequat chloride （800, 1200,1 600, 2 000, 2 400 rag/L） and paclobutracol （50, 75, 100, 125, 150 mg/L） on yield and quality of sunflower were investigated. The results showed that plant growth regulators could improve the yield and quality of sunflower seeds to varying degrees. Specifically, 400 mg/L mepiquat chloride （ D4 ）, 800 mg/L chlormequat chloride （ Cl ） and 75 mg/L paelobutracol （ P2 ） significantly improved disk diameter, seed weight per disc, 100-sced weight, seed kernel rate and yield of sunflower; 400 mg/L mepiquat chloride （ D4 ）, 1 200 mg/L chlormequat chloride （ C2 ） and 75 mg/L paclobutracol （ P2 ） improved crude protein content, crude fat content, length and width of sunflower seeds. Based on comprehensive consideration, paclobutraeol is the most appropriate plant growth regulator for improving the yield and quality of sunflower in Xinjiang, and the optimal application concentration is 75 mg/L.展开更多
In recent years,plant growth regulators are widely used in agricultural products.As the toxicity of plant growth regulator residues has gained increasing concerns,trace analysis methods for plant growth regulators hav...In recent years,plant growth regulators are widely used in agricultural products.As the toxicity of plant growth regulator residues has gained increasing concerns,trace analysis methods for plant growth regulators have been developed.In this paper,the major methods with advantages and disadvantages for the detection and pre-treatment of plant growth regulator residues in agricultural products were summarized,including gas chromatography(GC),high performance liquid chromatography(HPLC),chromatographic technique combined with mass spectrometry,enzyme-linked immunosorbent assay(ELISA),capillary electrophoresis(CE)and so on.Meanwhile,the development prospects were also discussed.展开更多
To achieve optimum yield of wheat, supplementation of soil and foliar applied fertilizers with plant growth regulators is vital to assure effective assimilation of nutrients by plants. A trial was conducted at Gwebi A...To achieve optimum yield of wheat, supplementation of soil and foliar applied fertilizers with plant growth regulators is vital to assure effective assimilation of nutrients by plants. A trial was conducted at Gwebi Agricultural College Farm in Mashonaland West Province of Zimbabwe, during the 2012 winter wheat season (May to August), to evaluate the effects of differing times of application of Tianda 2116 plant growth regulator on the growth and yield of wheat (variety SC Sekuru). The trial was laid out in a Randomized Complete Block Design with three replications. The treatments were: Tianda 2116 applied at the following weeks after planting (WAP): 2 WAP, 3 WAP, 4 WAP, 5 WAP, 6 WAP, 14 WAP and no Tianda 2116 applied (control). No significant differences (P > 0.05) were noted among treatments for mean number of tillers per plant and mean number of spikes per plant. Application of Tianda 2116 at 2 and 3 WAP delayed flowering and physiological maturity. Early application of Tianda 2116 increased the number of spikelets per spike, thousand grain weight and grain yield. Conclusively, Tianda 2116 applied 2 or 3 WAP is effective in reducing growth while enhancing yield parameters in wheat production.展开更多
Rice with polyembryony characterized bytwin seedlings is a good genetic tool for apomixisresearch.A study on the effect of five plantgrowth regulators(IAA,KT,GA,2,4-D and6-BAP)on rate of twin seedlings in 3 rice varie...Rice with polyembryony characterized bytwin seedlings is a good genetic tool for apomixisresearch.A study on the effect of five plantgrowth regulators(IAA,KT,GA,2,4-D and6-BAP)on rate of twin seedlings in 3 rice varie.ties with polyembryony(Shuang 3,Shuang 13and Lu 52)was conducted.The results showed展开更多
基金Supported by Basic Research Funds of Hebei Academy of Agriculture and Forestry Sciences(2023020103)Domestic Training Program for Young Scientific and Technological Talents of Agriculture and Forestry Sciences+3 种基金Hebei Agriculture Research System(HBCT2023150202)Key R&D Program Project of Hebei Provincial Department of Science and Technology(20326813D)The innovation project of modern seed technology(21326310D)HAAFS Science and Technology Innovation Special Project(2022KJCXZX-CGS-1).
文摘The effects of different treatments on the seedlessness and fruit quality of‘Miguang’table grape was studied by using plant growth regulators,gibberellin acid(GA 3)and forchlorfenuron(CPPU),under different concentrations and application time.The results showed that the effects of different treatments on the seedlessness and fruit quality were different.Seedless rate,cluster weight,berry weight,berry shape index,soluble solid content,total acid content,soluble solids to acidity ratio,pulling resistance,turgor pressure and flesh firmness without skin were comprehensively evaluated,as a result of which,the optimum treatment on‘Miguang’table grape was to apply with GA 320 mg/L+SM 200 mg/L at one week before bloom and GA 325 mg/L+CPPU 3 mg/L at two weeks after bloom.
文摘Global concerns about the environmental impact of combustion emissions from petroleum fuels influence new research to seek for alternative energy sources. The current study investigates the possibility of using safflower (Carthamus tinctorius L.) as an alternative biodiesel raw material. Four plant growth regulators (PGR) were used to boost the production of safflower. Thirteen treatments were constituted from the four plant regulators and applied to the safflower crop arranged in completely randomised design, repeated three times. The results show that the effect of plant growth regulators was not more than that of the control. More studies have to be channelled towards the relationship between safflower and plant growth regulators.
基金Supported by Key Technology Integration and Demonstration of Xinjiang Characteristic Fruit Trees'High Efficiency and Safe Production,Science and Technical Plan Project of Xinjiang Uygur Autonomous Region(201130102)Key Technology Integration and Demonstration of Xinjiang Apricot Industrial Development,Science and Technical Plan Project of Xinjiang Uygur Autonomous Region(200931101)Financial Support from Xinjiang Uygur Autonomous Region Fruit Trees Key Subject~~
文摘[Objective] This study aimed to investigate the influence of different plant growth regulators on apricot pollen germination and pollen tube growth. [Method] Pollens of six kinds of Xinjiang apricots were cultured in solid media supplemented with five plant growth regulators （GA3 , NAA, 2, 4-D, 6-BA, IAA）. Then the rate of pollen germination and the length of pollen tube were respectively measured. [Result] In a certain concentration range, GA3 most significantly promoted the pollen germination and the pollen tube growth of Shushanggan, Kalayulvke, Dayoujia, Yiliakeyulvke and Kabakehuanna; NNA had the strongest improvement function on Kumaiti’s pollen germination and pollen tube growth. [Conclusion] All the five plant growth regulators promoted the pollen germination and the pollen tube growth of apricots at low concentration but inhibited them at high concentration.
文摘The effects of four kinds of plant growth regulators with different concentrations on narcissi were studied in 2001. The results showed that the regulators could inhibit the growths of height and leaves of narcissi. Of the four regulators, the dwarfing effects of paclobatrazol (PP333) and uniconazole (S3307) on narcissi were better than those of chlorocholine (CCC) and dimethyl amino-sussinamic acid (B9). All of the regulators did not have significant effect on the root length. Moreover, the time of flowering was later for the narcissi treated with regulators than that of the control to a certain extent, and the range delayed was from 2 days to 19 days. The correlation analysis results showed that there was a significant correlation between the time of flowering and the concentrations of regulators. The ornament value of narcissi was obviously improved by using the regulators.
基金Supported by program from Tobacco Monopoly Bureau of Guangxi Zhuang Nationality Autonomous Region(Research and Demonstration of Comprehensive Technique for Enhancing the Absorption and Accumulation of Potassium in Flue-cured Tobacco)Science and Research Fund from Guangxi University(x071057)Innovation Project of Guangxi Graduate Education(2008105930903M012)~~
文摘[Objective] This study was to investigate the effects of plant growth regulator on accumulation and circulation of potassium in flue-cured tobacco.[Methods] Hydroponics experiment was adopted to study the effects of plant growth regulator（NAA,GA3,BR） on accumulation and circulation of potassium in flue-cured tobacco.[Results] The BR treatment increased the absorption of potassium,calcium and magnesium in flue-cured tobacco,reduced the potassium emission from the root,enhanced the accumulation and contents and promoted the circulation of potassium in the tobacco plants.There was no statistical difference between the NAA and GA3 treatment.And BR treatment was 0.52%,0.30%,0.28% higher than NAA treatment in enhancing potassium content in tobacco plants at 2,7,12 d after topping.In a word,the results showed that BR treatment was the most.[Conclusion] BR treatment could effectively enhance potassium content in tobacco plants after topping.
文摘[Objective]In order to increase anther culture efficiency of pepper.[Method]MS culture media and Bolajiaohong were used in this experiment to study the influences of carbon sources and concentrations on anther callus induction of pepper.Jiayu was taken as a material to study influences of plant growth regulators and concentrations on anther callus induction of pepper according to L16（4^5） orthogonal design.[Result]The average callus and embryoid induction rates of maltose at all concentrations were higher than these of sucrose but the difference was not significant.Taking maltose or sucrose as a carbon source,3% to 6% concentration was good for increasing induction frequencies of calli and embryoids.However,If the concentration was over 6%,the induction rates were declined dramatically with the increase of sugar concentration.The influences of growth regulators on induction rate of calli were listed as 2,4-D﹥ZT﹥NAA﹥KT﹥6-BA;the influences on induction rates of embryoids were listed as 2,4-D﹥NAA﹥ZT﹥KT﹥6-BA.The 2,4-D,ZT,NAA and KT had signficant or extremely significant influences on induction rates of calli and embryoids.2,4-D,ZT at 1.0 mg/L and NNA,KT at 0.5 mg/L had the best effects.The influences of ZT on calli and embryoids were better than those of KT and 6-BA.1.0 mg/L 2,4-D ＋1.0 mg/L ZT ＋0.5 mg/L KT ＋0.5 mg/L 6-BA was the best regulator combination for induction culture of Jiayu anther.[Conclusion]The experiment provided research basis for anther culture of pepper.
文摘The effect of vernalization and two growth regulators Fascination^TM and Pro-Gibb^R on the growth, inflorescence development and flowering of Omithogalurrt Chesapeake Snowflake＇ was studied. Regardless of growth regulator treatment, chilling bulbs for 3 weeks at 10℃ before planting accelerated flowering of the first inflorescence by 5 to 6 days, elongated floral stem length by 1.5 to 2 cm and shortened leaf length by 4 to 5 cm as compared with non-chilled bulbs. When bulbs were chilled 3 weeks at 10℃ before planting, Fascination 2% and Pro-Gibb accelerated flowering of the first inflorescence by 2 to 6 days respectively as compared with the control. When bulbs were not chilled before planting, Fascination 2% and Pro-Gibb accelerated flowering of the first inflorescence by 6 to12 days respectively as compared with the control. The lengths of the leaves and the inflorescences were not affected by PGR treatment. Higher rates of Pro-Gibb （100 mg.L^-1 and 200 mg.L^-1） and more amount of Fascination 2%（100 μL and 200 μL） can cause abortion of inflorescence.
基金Supported by Daqing Scientific and Technological Project in Heilongjiang Province(SGG2007-054)Demonstration and Promotion Projects of Potato Comprehensive High Yield Cultivation Technologyin Western Regions of Heilongjiang Province(PC07B09)~~
文摘[Objective] The aim was to investigate the effects of plant growth regulators on contents of reducing sugar and starch in potato（Solanum tuberosum L.）.[Method]A potato cultivar named ＇Holand-212＇ with three treatments by spraying Diethyl aminoethyl hexanoate（DTA-6）,Uniconazole（S3307）and SOD mimics（SODM）were employed to compare differences of the contents of reducing sugar and starch in potato leaves,stolons and tubers in a field experiment.[Result]DTA-6 treatment could significantly reduce the contents of starch and reducing sugar in potato stolons in the period of 100-110 d after seeding,and it could also reduce the content of reducing sugar in tubers,but there is an opposite result in leaves in the same period,significantly.S3307 could extremely significantly increase the content of starch,and can promote the transfer of starch in stolons,in addition,it also could reduce the content of reducing sugar in tubers,and extremely significantly increase the content of starch in leaves at the 110 d after sowing.Moreover,there is a similar effect between SODM and S3307.[Conclusion]The research results are significant for understanding mechanisms of plant growth regulators and promoting application of plant growth regulator in potato.
基金supported by the Universiti Putra Malaysia Research University Grant Scheme(Project No.03-02-11-1370RU and 03-03-11-1438RU)
文摘The endangered tropical tree, Aquilaria malaccensis, produces agarwood for use in fragrance and medicines. Efforts are currently un-derway to produce valuable agarwood compoundsn tissue culture. The purpose of this study was to develop an optimal growth medium, specif-ically, the best hormone combination for callus suspension culture. Using nursery-grown A. malaccensis, sterilized leaf explants were first incu-bated on basic Murashige and Skoog （MS） gel medium containing 15g/L sucrose and at pH 5.7. Different auxin types including 1-naphthaleneacetic acid （NAA）, 2,4-dichlorophenoxyacetic acid （2,4-D）, and indole-3-butyric acid （IBA）, were tested at various concentrations （0.55, 1.1 and 1.65 μM） using the basic medium. Leaf explants were incubated for 30 days in the dark. Callus induced by 1.1 μM NAA had the highest biomass dry weight （DW） of 17.3 mg;however the callus was of a compact type. This auxin concentration was then combined with either 6-benzylaminopurine （BAP） or kinetin at 0.55, 1.1, 2.2 or 3.3 μM to induce growth of friable callus. The 1.1μM NAA＋2.2μM BAP com-bination produced friable callus with the highest biomass （93.3mg DW）. When testing the different carbon sources and pHs, sucrose at 15g/L and pH at 5.7 yielded highest biomasses at 87.7mg and 83mg DW, respec-tively. Microscopic observations revealed the arrangement of the friable cells as loosely packed with relatively large cells, while for the compact callus, the cells were small and densely packed. We concluded that MS medium containing 15 g/L sucrose, 1.1 μM NAA ＋ 2.2 μM BAP hor-mone combination, and a pH of 5.7 was highly effective for inducing friable callus from leaf explants of A. malaccensis for the purpose of establishing cell suspension culture.
基金Supported by Talent Introduction Project of Anhui Science and Technology University(ZRC2007147)General Project of Natural Scientific Research from Education Department of Anhui Province(KJ2010B052,KJ2010B294)
文摘In this study,the seeds of wild Petunia Juss.were used as explants to investigate the optimal condition for tissue culture.Several different kinds and concentrations of growth regulators were adopted to produce more multiple bud clumps,callus or roots in this study.The experiments may provide experimental foundation for the rapid propagation technology and establishment of tissue culture system for wild Petunia Juss.
基金supported by the National Natural Science Foundation of China(No.2012BAD20B04)
文摘The title compound 1-（3-amino-[1,2,4]triazol-1-yl）-3,3-dimethyl-butan-2-one（3） was synthesized by Hofmann-alkylation reaction of 1-chloro-3,3-dimethyl-butan-2-one（1） and ~1H-[1,2,4]triazol-3-ylamine（2） with equal amount of K_2CO_3 as acid acceptor. The structure of compound 3 was characterized by ~1H NMR, 13 C NMR, HRMS and single-crystal X-ray diffraction. The compound crystallizes in the monoclinic system, space group P21/n with a = 5.7227（8）, b = 27.924（4）, c = 6.2282（7） ？, β = 101.892（11）°, V = 973.9（2） ？~3, Z = 4, T = 180.00（10） K, μ（MoKα） = 0.087 mm^（-1）, Dc = 1.243 g/cm^3, 3832 reflections measured（3.648≤θ≤26.022°）, 1916 unique reflections（Rint = 0.0359, Rsigma = 0.0572） used in all calculations. The final R = 0.0557（I 〉 2σ（I）） and w R = 0.1276（all data）. Bioassay showed that 3 displayed excellent activity as plant growth regulator with inducing lateral root formation and enhancing primary root elongation at 0.27 mmol/L（50 ppm） in soybeen（He Feng-50）. Good water solubility was found with 50 mg in 1 m L of water. Therefore, application of 3 in agriculture is more environmentally friendly due to cosolvent-free condition, and results in improved abiotic-stress tolerance by affecting the root growth. And furthermore, it can be used as a precursor to investigate the function of regulating plant root growth.
文摘A stereocontrolled construction of the side chain of brassinclide and homobrassinolide has been achieved via the tandem vicinal dialkylation of the pyranone moiety as a key step.
基金Supported by Research Projects Funded by Talent Project Training Funds in Hebei Province(A201901128)Key R&D Project of Tangshan City(20150210C).
文摘[Objectives]This study was conducted to effectively monitor PGR residues in bean sprouts to provide guarantee for the food safety of agricultural products.[Methods]A high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for the determination of residues of 15 plant growth regulators(PGRs)in bean sprouts was established using bean sprouts as an experimental material.Samples were extracted with a solution containing 5%acetic acid-acetonitrile(1∶99,V/V),purified with anhydrous magnesium sulfate,and diluted with methanol solvent to constant volume.The solutions were filtered through 0.22μm filtering membrane and the target analytes were separated on a Phenomenex H18 column.The identification of each compound was established by retention time matching along with the accurate mass measurement of the precursor ions and their main fragment ions.The quantification was carried out using matrix-matched external standard method.[Results]The retention time of the 15 PGRs were found in the range from 5.8-11.7 min under the optimized conditions.The linear relation was good in the concentration range of 0.005-0.050μg/ml,and the correlation coefficients of the 15 PGRs were≥0.9990.The limits of detection were in the range of 0.03-0.92 g/kg,and the limits of quantification were in the range of 0.50-2.10μg/kg.The average recovery in the recovery test at 3 concentration levels was 80%-110%,and the relative standard deviations were in the range of 2.8%-7.5%.[Conclusions]This method is simple and accurate,and can quickly qualitatively and quantitatively analyze the residues of 15 PGRs in bean sprouts.The proposed procedure was simple,quick and accurate for the simultaneous determination of the 15 PGRs in bean sprout.
文摘Tests of new natural non-polluting regulators of growth Verva （vegetative terpenoids from Abies sibirica） and Larixin （vegetative flavonoids from Larix） on growth, development and productivity of fibre flax are conducted. The level of phytohormones in vegetating plants （IAA, ABA, zeatine and zeatinriboside） is studied. Preparations stimulate germination of seeds and growth of plants. Field experiment fixed yield increase of flax straw and the seeds of flax-fibre with the use of growth regulators. Industrial experiment represents VERVA product effectiveness on yields increase of flax straw, seeds and flax fiber output.
基金supported by the Department of Biotechnology,Government of India New Delhithe World Academy of Science(TWAS)for the Advancement of Science in the Developing World TriesteItaly through DBT-TWAS Postgraduate Research Fellowship
文摘Chonemorpha fragrans is an endangered medicinal woody climber,regarded among alternative plant sources of camptothecin.Camptothecin is a monoterpene indole anti-cancer alkaloid with annual trade value of over three billion U.S.dollars in the recent,and is used in the production of its analog drugs approved for the chemotherapy of cancer of varied types.Effects of plant growth regulators,culture media strength and photoperi-odic duration on the micropropagation ef ciency of C.fragrans from nodal segment explants were studied on Murashige and Skoog(MS)medium amended with Thidiazuron(TDZ),Benzylaminopurine(BAP)or Kinetin(Kin).Thidiazuron was more ef cient over BAP and Kin when half basal MS medium was used over full or quarter strength.Results of carbon source experiment showed sucrose as the most effective over glucose,fructose,and maltose in the clonal production.Studies on the photope-riodic incubation duration showed 12 h as the best light period and sub or supra-optimal resulted in the production of abnormal and albino micro shoots.Experimental results on the evaluation of physiological,biochemical parameters showed the role of pigment molecules and antioxidant systems in the production of albino micro shoots.
基金Supported by the Vegetable Innovation Team Project of Shandong Modern Agricultural Industry Technology System(SDAIT-05-09)Shandong Major Agricultural Technology Innovation Projects in 2014Shandong Province Agricultural Promotion Program in 2015
文摘Most plant growth regulators available are synthesized and tend to be dangerous if used inappropriately. Emistim CAS is a natural plant growth regulator, with less side-effect. The research performed an assessment on Emistim CAS application in watermelon cultivation in a greenhouse. The results showed that 0.11% Emistim CAS increased reducing sugar by 15.49%, soluble solid by 13.7%, Vc content by 3.72%, titratable acid by 18.1%, and yield per hectare by 18.01%, so that watermelon quality and yield were both improved. Emistim CAS can be widely promoted in watermelon cultivation in future.
基金Supported by Modern Agricultural Science and Technology Research and Achievements Transformation Fund of Xinjiang Production and Construction Corps(2016AC027,2016AC024)
文摘Using Xinshikui No. 5 as the experimental material, the effects of different concentrations of mepiquat chloride （100, 200, 300,400, 500 mg/L）, chlormequat chloride （800, 1200,1 600, 2 000, 2 400 rag/L） and paclobutracol （50, 75, 100, 125, 150 mg/L） on yield and quality of sunflower were investigated. The results showed that plant growth regulators could improve the yield and quality of sunflower seeds to varying degrees. Specifically, 400 mg/L mepiquat chloride （ D4 ）, 800 mg/L chlormequat chloride （ Cl ） and 75 mg/L paelobutracol （ P2 ） significantly improved disk diameter, seed weight per disc, 100-sced weight, seed kernel rate and yield of sunflower; 400 mg/L mepiquat chloride （ D4 ）, 1 200 mg/L chlormequat chloride （ C2 ） and 75 mg/L paclobutracol （ P2 ） improved crude protein content, crude fat content, length and width of sunflower seeds. Based on comprehensive consideration, paclobutraeol is the most appropriate plant growth regulator for improving the yield and quality of sunflower in Xinjiang, and the optimal application concentration is 75 mg/L.
基金Tangshan Science and Technology Planning Project(20150210C)Hebei Provincial Phase II Modern Agricultural Industry Technology System Innovation Team Building Project(HBCT2018120207,HBCT2018160403).
文摘In recent years,plant growth regulators are widely used in agricultural products.As the toxicity of plant growth regulator residues has gained increasing concerns,trace analysis methods for plant growth regulators have been developed.In this paper,the major methods with advantages and disadvantages for the detection and pre-treatment of plant growth regulator residues in agricultural products were summarized,including gas chromatography(GC),high performance liquid chromatography(HPLC),chromatographic technique combined with mass spectrometry,enzyme-linked immunosorbent assay(ELISA),capillary electrophoresis(CE)and so on.Meanwhile,the development prospects were also discussed.
文摘To achieve optimum yield of wheat, supplementation of soil and foliar applied fertilizers with plant growth regulators is vital to assure effective assimilation of nutrients by plants. A trial was conducted at Gwebi Agricultural College Farm in Mashonaland West Province of Zimbabwe, during the 2012 winter wheat season (May to August), to evaluate the effects of differing times of application of Tianda 2116 plant growth regulator on the growth and yield of wheat (variety SC Sekuru). The trial was laid out in a Randomized Complete Block Design with three replications. The treatments were: Tianda 2116 applied at the following weeks after planting (WAP): 2 WAP, 3 WAP, 4 WAP, 5 WAP, 6 WAP, 14 WAP and no Tianda 2116 applied (control). No significant differences (P > 0.05) were noted among treatments for mean number of tillers per plant and mean number of spikes per plant. Application of Tianda 2116 at 2 and 3 WAP delayed flowering and physiological maturity. Early application of Tianda 2116 increased the number of spikelets per spike, thousand grain weight and grain yield. Conclusively, Tianda 2116 applied 2 or 3 WAP is effective in reducing growth while enhancing yield parameters in wheat production.
文摘Rice with polyembryony characterized bytwin seedlings is a good genetic tool for apomixisresearch.A study on the effect of five plantgrowth regulators(IAA,KT,GA,2,4-D and6-BAP)on rate of twin seedlings in 3 rice varie.ties with polyembryony(Shuang 3,Shuang 13and Lu 52)was conducted.The results showed
