Early diagnosis of pregnancy plays an important role to minimize reproductive losses in farm animals.There are several methods for pregnancy diagnosis like profiling of reproductive hormones(such as progesterone and e...Early diagnosis of pregnancy plays an important role to minimize reproductive losses in farm animals.There are several methods for pregnancy diagnosis like profiling of reproductive hormones(such as progesterone and estrone sulfate),but sometimes they provide false-positive results.Embryo specific pregnancy markers,which delineate the presence and viability of the embryo,are considered as perfect for pregnancy determination.Pregnancy-associated glycoproteins are distinguished as the best indicator for the determination of early pregnancy,fetal number,and birth weight of kids.Pregnancy-associated glycoproteins are structurally correlated to aspartic proteinase and are communicated in the external epithelial cell layer of the placenta.They have been found to share about half amino acid sequence identity with pepsinogen,pepsin,cathepsin D and E.Dislike different individuals from aspartic proteinase family,numerous pregnancy-associated glycoproteins appear to be latent compound as a result of amino acid substitutions in and around the catalytic site.This review is to discuss the scope and prospects of pregnancy-associated glycoproteins as a pregnancy marker in farm animals,more specifically in goats.展开更多
Accumulating studies have demonstrated that non-coding RNAs(ncRNAs),functioning as important regulators of transcription and translation,are involved in the establishment and maintenance of pregnancy,especially the ma...Accumulating studies have demonstrated that non-coding RNAs(ncRNAs),functioning as important regulators of transcription and translation,are involved in the establishment and maintenance of pregnancy,especially the maternal immune adaptation process.The endometrial stromal cells(ESCs),trophoblast cells,and decidua immune cells that reside at the maternal-fetal interface are thought to play significant roles in normal pregnancy and pregnancy-associated diseases.Here,we reviewed the up-to-date evidence on how microRNA,long non-coding RNA,and circular RNA regulate ESCs,trophoblast cells,and immune cells and discussed the potential applications of these ncRNAs as diagnostic and therapeutic markers in pregnancy complications.展开更多
Background:Pregnancy-associated breast cancer(PABC)is a special type of breast cancer that occurs during pregnancy and within 1 year after childbirth.With the rapid social development and the adjustment of reproductiv...Background:Pregnancy-associated breast cancer(PABC)is a special type of breast cancer that occurs during pregnancy and within 1 year after childbirth.With the rapid social development and the adjustment of reproductive policies in China,the average age of females at first childbirth is increasing,which is expected to lead to an increase in the incidence of PABC.This study aimed to accumulate clinical experience and to investigate and summarize the prevalence,diagnosis,and treatment of PABC based on large multicenter samples in China.Methods:According to the Chinese Society of Breast Surgery,a total of 164 patients with PABC in 27 hospitals from January 2016 to December 2018 were identified.The pregnancy status,clinicopathological features,comprehensive treatment methods,and outcomes were retrospectively analyzed.Survival curves were plotted using the Kaplan-Meier method.Results:A total of 164 patients of PABC accounted for 0.30%of the total number of cases in the same period;of which,83 patients were diagnosed during pregnancy and 81 patients during lactation.The median age of PABC was 33 years(24–47 years).Stage Ⅰ patients accounted for 9.1%(15/164),stage Ⅱ 54.9%(90/164),stage Ⅲ 24.4%(40/164),and stage Ⅳ 2.4%(4/164).About 9.1%(15/164)of patients were luminal A.Luminal B patients accounted the most(43.3%[71/164]).About 15.2%(25/164)of patients were human epidermal growth factor receptor 2(Her-2)overexpression and 18.9%(31/164)of patients were triple-negative breast cancer.For pregnancy breast cancer,36.1%(30/83)of patients received direct surgery and 20.5%(17/83)received chemotherapy during pregnancy.About 31.3%(26/83)chose abortion or induction of labor.The median follow-up time was 36 months(3–59 months);11.0%(18/164)patients had local recurrence or distant metastasis and 3.0%(5/164)died.Conclusions:It is safe and feasible to standardize surgery and chemotherapy for PABC.展开更多
BACKGROUND Gestational diabetes mellitus(GDM)is a concern due to its rapid increase in incidence in recent years.AIM To investigate the correlation and predictive value of serum pregnancyassociated plasma protein A(PA...BACKGROUND Gestational diabetes mellitus(GDM)is a concern due to its rapid increase in incidence in recent years.AIM To investigate the correlation and predictive value of serum pregnancyassociated plasma protein A(PAPP-A),triglyceride(TG),and 25-hydroxyvitamin D[25-(OH)D]with GDM in early pregnancy.METHODS A total of 99 patients in early pregnancy admitted to Peking University International Hospital from November 2015 to September 2017 were included,and underwent a fasting glucose test and oral glucose tolerance test screening at 24-28 wk of pregnancy.Of these cases with GDM,51 were assigned to group A and the remaining 48 cases without GDM were enrolled in group B.Serum PAPP-A,TG and 25-(OH)D in the two groups were compared and their correlation with blood sugar was analyzed.In addition,their diagnostic value in GDM was determined using receiver operating characteristic(ROC)curve analysis.RESULTS Group A had markedly lower serum PAPP-A and 25-(OH)D levels and a significantly higher serum TG level than group B,with statistical significance(P<0.05).Furthermore,Pearson analysis identified that PAPP-A and 25-(OH)D levels were negatively correlated with fasting blood glucose(FBG)levels(r=-0.605,P<0.001),(r=-0.597,P<0.001),while TG and FBG levels were positively correlated(r=0.628,P<0.001).The sensitivity,specificity,area under the curve(AUC)and optimal cut-off value of serum PAPP-A level in the diagnosis of GDM were 72.55%,82.35%,0.861 and 16.340,respectively,while the sensitivity of TG in diagnosing GDM was 86.27%,the specificity was 66.67%,the AUC was 0.813,with an optimal cut-off value of 1.796.The corresponding sensitivity,specificity,AUC and optimal cut-off value of serum 25-(OH)D were 64.71%,70.59%,0.721 and 23.140,respectively.Moreover,multivariate logistic regression analysis revealed that FBG,vascular endothelial growth factor,Flt-1,serum PAPP-A,TG,and 25-(OH)D were related risk factors leading to GDM in patients.CONCLUSION Serum PAPP-A,TG,and 25-(OH)D levels are all correlated with blood glucose changes in GDM,and are independent factors affecting the occurrence of GDM and have certain value in the diagnosis of GDM.展开更多
Background: The consequences of malaria during pregnancy are different regarding local conditions of malaria transmission. In stable malaria areas, the main complications are maternal anaemia and fetal growth restrict...Background: The consequences of malaria during pregnancy are different regarding local conditions of malaria transmission. In stable malaria areas, the main complications are maternal anaemia and fetal growth restriction. This study aims to determine if pregnancy-associated malaria is associated with the risk of the above-mentioned complications and to determine if IPTp-sp reduces them in Kisangani. Methods: It is a cross-sectional analytical study conducted in parturients, in 6 medical facilities of Kisangani, from January 1st to September 30th, 2017. At delivery we measured their hemoglobin, we performed the thick blood smear of their peripheral blood and placental apposition;and we weighed their newborns at birth. Results: Risk of anaemia at delivery increased with malaria access during pregnancy (p = 0.0056;OR: 1.4221, 95% CI: 1.0851 - 1.8638) and peripheral parasitaemia at delivery (p = 0.0000;OR: 6.3855, 95% CI: 4.5552 - 8.9512). LBW increased with peripheral parasitaemia at delivery (p = 0.0000;OR: 3.5299, 95% CI: 2.4424 - 5.1015) and placental parasitaemia (p = 0.0000;OR: 18.3247, 95% CI: 12.5141 - 26.8332). IPTp-sp did not have effect on maternal hemoglobin at delivery (p = 0.1546;OR: 0.7553, IC à 95%: 0.4414 - 1.2923) and the birth weight (p = 0.1225;OR: 0.6638, IC à 95%: 0.3375 - 1.3056). Conclusion: In Kisangani, pregnancy-associated malaria is associated with maternal anaemia at delivery and LBW. IPTp-sp does not reduce the risk of these complications. Therefore, studies evaluating IPTp alternatives are required in malaria endemic areas.展开更多
文摘Early diagnosis of pregnancy plays an important role to minimize reproductive losses in farm animals.There are several methods for pregnancy diagnosis like profiling of reproductive hormones(such as progesterone and estrone sulfate),but sometimes they provide false-positive results.Embryo specific pregnancy markers,which delineate the presence and viability of the embryo,are considered as perfect for pregnancy determination.Pregnancy-associated glycoproteins are distinguished as the best indicator for the determination of early pregnancy,fetal number,and birth weight of kids.Pregnancy-associated glycoproteins are structurally correlated to aspartic proteinase and are communicated in the external epithelial cell layer of the placenta.They have been found to share about half amino acid sequence identity with pepsinogen,pepsin,cathepsin D and E.Dislike different individuals from aspartic proteinase family,numerous pregnancy-associated glycoproteins appear to be latent compound as a result of amino acid substitutions in and around the catalytic site.This review is to discuss the scope and prospects of pregnancy-associated glycoproteins as a pregnancy marker in farm animals,more specifically in goats.
基金Natural Science Foundation of China(No.81873337)Natural Science Foundation of Shandong Province(No.ZR2019MH039)+2 种基金Central Government Guides Local Science and Technology Development Fund Projects of Shandong Province(No.YDZX20203700001407)Research Leader Studio Project of Jinan Science and Technology Bureau(No.2020GXRC050)Taishan Scholars(No.Tsqn201812125)
文摘Accumulating studies have demonstrated that non-coding RNAs(ncRNAs),functioning as important regulators of transcription and translation,are involved in the establishment and maintenance of pregnancy,especially the maternal immune adaptation process.The endometrial stromal cells(ESCs),trophoblast cells,and decidua immune cells that reside at the maternal-fetal interface are thought to play significant roles in normal pregnancy and pregnancy-associated diseases.Here,we reviewed the up-to-date evidence on how microRNA,long non-coding RNA,and circular RNA regulate ESCs,trophoblast cells,and immune cells and discussed the potential applications of these ncRNAs as diagnostic and therapeutic markers in pregnancy complications.
文摘Background:Pregnancy-associated breast cancer(PABC)is a special type of breast cancer that occurs during pregnancy and within 1 year after childbirth.With the rapid social development and the adjustment of reproductive policies in China,the average age of females at first childbirth is increasing,which is expected to lead to an increase in the incidence of PABC.This study aimed to accumulate clinical experience and to investigate and summarize the prevalence,diagnosis,and treatment of PABC based on large multicenter samples in China.Methods:According to the Chinese Society of Breast Surgery,a total of 164 patients with PABC in 27 hospitals from January 2016 to December 2018 were identified.The pregnancy status,clinicopathological features,comprehensive treatment methods,and outcomes were retrospectively analyzed.Survival curves were plotted using the Kaplan-Meier method.Results:A total of 164 patients of PABC accounted for 0.30%of the total number of cases in the same period;of which,83 patients were diagnosed during pregnancy and 81 patients during lactation.The median age of PABC was 33 years(24–47 years).Stage Ⅰ patients accounted for 9.1%(15/164),stage Ⅱ 54.9%(90/164),stage Ⅲ 24.4%(40/164),and stage Ⅳ 2.4%(4/164).About 9.1%(15/164)of patients were luminal A.Luminal B patients accounted the most(43.3%[71/164]).About 15.2%(25/164)of patients were human epidermal growth factor receptor 2(Her-2)overexpression and 18.9%(31/164)of patients were triple-negative breast cancer.For pregnancy breast cancer,36.1%(30/83)of patients received direct surgery and 20.5%(17/83)received chemotherapy during pregnancy.About 31.3%(26/83)chose abortion or induction of labor.The median follow-up time was 36 months(3–59 months);11.0%(18/164)patients had local recurrence or distant metastasis and 3.0%(5/164)died.Conclusions:It is safe and feasible to standardize surgery and chemotherapy for PABC.
文摘BACKGROUND Gestational diabetes mellitus(GDM)is a concern due to its rapid increase in incidence in recent years.AIM To investigate the correlation and predictive value of serum pregnancyassociated plasma protein A(PAPP-A),triglyceride(TG),and 25-hydroxyvitamin D[25-(OH)D]with GDM in early pregnancy.METHODS A total of 99 patients in early pregnancy admitted to Peking University International Hospital from November 2015 to September 2017 were included,and underwent a fasting glucose test and oral glucose tolerance test screening at 24-28 wk of pregnancy.Of these cases with GDM,51 were assigned to group A and the remaining 48 cases without GDM were enrolled in group B.Serum PAPP-A,TG and 25-(OH)D in the two groups were compared and their correlation with blood sugar was analyzed.In addition,their diagnostic value in GDM was determined using receiver operating characteristic(ROC)curve analysis.RESULTS Group A had markedly lower serum PAPP-A and 25-(OH)D levels and a significantly higher serum TG level than group B,with statistical significance(P<0.05).Furthermore,Pearson analysis identified that PAPP-A and 25-(OH)D levels were negatively correlated with fasting blood glucose(FBG)levels(r=-0.605,P<0.001),(r=-0.597,P<0.001),while TG and FBG levels were positively correlated(r=0.628,P<0.001).The sensitivity,specificity,area under the curve(AUC)and optimal cut-off value of serum PAPP-A level in the diagnosis of GDM were 72.55%,82.35%,0.861 and 16.340,respectively,while the sensitivity of TG in diagnosing GDM was 86.27%,the specificity was 66.67%,the AUC was 0.813,with an optimal cut-off value of 1.796.The corresponding sensitivity,specificity,AUC and optimal cut-off value of serum 25-(OH)D were 64.71%,70.59%,0.721 and 23.140,respectively.Moreover,multivariate logistic regression analysis revealed that FBG,vascular endothelial growth factor,Flt-1,serum PAPP-A,TG,and 25-(OH)D were related risk factors leading to GDM in patients.CONCLUSION Serum PAPP-A,TG,and 25-(OH)D levels are all correlated with blood glucose changes in GDM,and are independent factors affecting the occurrence of GDM and have certain value in the diagnosis of GDM.
基金Faculty of Medicine and Pharmacy of University of Kisangani
文摘Background: The consequences of malaria during pregnancy are different regarding local conditions of malaria transmission. In stable malaria areas, the main complications are maternal anaemia and fetal growth restriction. This study aims to determine if pregnancy-associated malaria is associated with the risk of the above-mentioned complications and to determine if IPTp-sp reduces them in Kisangani. Methods: It is a cross-sectional analytical study conducted in parturients, in 6 medical facilities of Kisangani, from January 1st to September 30th, 2017. At delivery we measured their hemoglobin, we performed the thick blood smear of their peripheral blood and placental apposition;and we weighed their newborns at birth. Results: Risk of anaemia at delivery increased with malaria access during pregnancy (p = 0.0056;OR: 1.4221, 95% CI: 1.0851 - 1.8638) and peripheral parasitaemia at delivery (p = 0.0000;OR: 6.3855, 95% CI: 4.5552 - 8.9512). LBW increased with peripheral parasitaemia at delivery (p = 0.0000;OR: 3.5299, 95% CI: 2.4424 - 5.1015) and placental parasitaemia (p = 0.0000;OR: 18.3247, 95% CI: 12.5141 - 26.8332). IPTp-sp did not have effect on maternal hemoglobin at delivery (p = 0.1546;OR: 0.7553, IC à 95%: 0.4414 - 1.2923) and the birth weight (p = 0.1225;OR: 0.6638, IC à 95%: 0.3375 - 1.3056). Conclusion: In Kisangani, pregnancy-associated malaria is associated with maternal anaemia at delivery and LBW. IPTp-sp does not reduce the risk of these complications. Therefore, studies evaluating IPTp alternatives are required in malaria endemic areas.
