Objective To summarize the experience of 20 years in using hypertonic citrate adenine(HC-A) for clinical kidney preservation. Methods A total of 2 086 cases of kidneys for transplantation from Angust 1980 to December ...Objective To summarize the experience of 20 years in using hypertonic citrate adenine(HC-A) for clinical kidney preservation. Methods A total of 2 086 cases of kidneys for transplantation from Angust 1980 to December 2000 were reported. The donors’ age ranged from 18 to 49 years. The cadaveric kidneys were harvested with en-bloc method, and were perfused via renal arteries with 250 ml HC- A at 2 - 4 ℃ and 10 cm H2O, and then stored in HC- A at 2 - 4 ℃ throughout preservation. Serum creatinine was measured at 1 week and 1 month after transplantation. Graft survival rates at 1 year and 3,5,10 years and mean graft survival periods were followed up. According to the cold ischemic time, the cases were divided into 3 groups; Group 1, n = 573, with cold ischemic time less than 12 h; Group 2, n = 779, with 12 to 24 h; and Group 3, n = 734, with more than 24 h. Results The mean cold ischemic period of 2 086 cases was(23.68±4.51) h. The mean level of serum creatinine at 1 week and 1 month after展开更多
Heart transplantation is now being accepted worldwide and has presented with good results. However,the shortage of donor hearts has become an obstacle of heart transplantation. To explore the donor heart sources and t...Heart transplantation is now being accepted worldwide and has presented with good results. However,the shortage of donor hearts has become an obstacle of heart transplantation. To explore the donor heart sources and to prolong the preservation time, we chose the adult porcine heart which is similar to human’s in size, weight, coronary architecture and conduction system for experiment. In part I, a simple cold flushing and storage method was used for preservation of 11 porcine hearts for 4-6 hours. Before a heart procurement, the carotid artery and the jugular vein were cut for bleeding and collecting a total amount of 2000ml of blood. A heart-lung machine was primed with diluted blood. Before resuscitation, the heart was reperfused with warm blood cardioplegic solution to reduce reperfusion injury, and then with the normal展开更多
Iron water treatment residues （Fe-WTR） are a free by-product of the treatment of drinking water with high concentration of iron oxides and potential for arsenic sorption. This paper aims at applying Fe- WTR to a con...Iron water treatment residues （Fe-WTR） are a free by-product of the treatment of drinking water with high concentration of iron oxides and potential for arsenic sorption. This paper aims at applying Fe- WTR to a contaminated site, measuring the reduction in contaminant leaching, and discussing the design of delivery and mixing strategy for soil stabilization at field scale and present a cost-effective method of soil mixing by connnon contractor machinery. Soil contaminated by As, Cr, and Cu at an abandoned wood impregnation site was amended with 0.22% （dw） Fe-WTR. To evaluate the full scale amendment a 100 mtest site and a control site （without amendment） were monitored for 14 months. Also soil analysis of Fe to evaluate the degree of soil and Fe-WTR mixing was done. Stabilization with Fe-WTR had a significant effect on leachable contaminants, reducing pore water As by 93%, Cu by 91% and Cr by 95% in the upper samplers. Dosage and mixing of Fe-WTR in the soil proved to be difficult in the deeper part of the field, and Pire water concentrations of arsenic was generally higher.Despite water logged conditions no increase in dissolved iron or arsenic was observed in the amended soil. Our field scale amendment of contaminated soil was overall successful in decreasing leaching of As, Cr and Cu. With minor improvements in the mixing and delivery strategy, this stabilization method is suggested for use in cases, where leaching ofCu, Cr and As constitutes a risk for groundwater and freshwater.展开更多
文摘Objective To summarize the experience of 20 years in using hypertonic citrate adenine(HC-A) for clinical kidney preservation. Methods A total of 2 086 cases of kidneys for transplantation from Angust 1980 to December 2000 were reported. The donors’ age ranged from 18 to 49 years. The cadaveric kidneys were harvested with en-bloc method, and were perfused via renal arteries with 250 ml HC- A at 2 - 4 ℃ and 10 cm H2O, and then stored in HC- A at 2 - 4 ℃ throughout preservation. Serum creatinine was measured at 1 week and 1 month after transplantation. Graft survival rates at 1 year and 3,5,10 years and mean graft survival periods were followed up. According to the cold ischemic time, the cases were divided into 3 groups; Group 1, n = 573, with cold ischemic time less than 12 h; Group 2, n = 779, with 12 to 24 h; and Group 3, n = 734, with more than 24 h. Results The mean cold ischemic period of 2 086 cases was(23.68±4.51) h. The mean level of serum creatinine at 1 week and 1 month after
文摘Heart transplantation is now being accepted worldwide and has presented with good results. However,the shortage of donor hearts has become an obstacle of heart transplantation. To explore the donor heart sources and to prolong the preservation time, we chose the adult porcine heart which is similar to human’s in size, weight, coronary architecture and conduction system for experiment. In part I, a simple cold flushing and storage method was used for preservation of 11 porcine hearts for 4-6 hours. Before a heart procurement, the carotid artery and the jugular vein were cut for bleeding and collecting a total amount of 2000ml of blood. A heart-lung machine was primed with diluted blood. Before resuscitation, the heart was reperfused with warm blood cardioplegic solution to reduce reperfusion injury, and then with the normal
文摘Iron water treatment residues （Fe-WTR） are a free by-product of the treatment of drinking water with high concentration of iron oxides and potential for arsenic sorption. This paper aims at applying Fe- WTR to a contaminated site, measuring the reduction in contaminant leaching, and discussing the design of delivery and mixing strategy for soil stabilization at field scale and present a cost-effective method of soil mixing by connnon contractor machinery. Soil contaminated by As, Cr, and Cu at an abandoned wood impregnation site was amended with 0.22% （dw） Fe-WTR. To evaluate the full scale amendment a 100 mtest site and a control site （without amendment） were monitored for 14 months. Also soil analysis of Fe to evaluate the degree of soil and Fe-WTR mixing was done. Stabilization with Fe-WTR had a significant effect on leachable contaminants, reducing pore water As by 93%, Cu by 91% and Cr by 95% in the upper samplers. Dosage and mixing of Fe-WTR in the soil proved to be difficult in the deeper part of the field, and Pire water concentrations of arsenic was generally higher.Despite water logged conditions no increase in dissolved iron or arsenic was observed in the amended soil. Our field scale amendment of contaminated soil was overall successful in decreasing leaching of As, Cr and Cu. With minor improvements in the mixing and delivery strategy, this stabilization method is suggested for use in cases, where leaching ofCu, Cr and As constitutes a risk for groundwater and freshwater.
