Citrus is an important fruit in Sichuan,accounting for 52.6%fruit yield of the whole Sichuan Province.In recent years,citrus has expanded its original cultivation and planting area in Sichuan,and the planting area has...Citrus is an important fruit in Sichuan,accounting for 52.6%fruit yield of the whole Sichuan Province.In recent years,citrus has expanded its original cultivation and planting area in Sichuan,and the planting area has continued to increase.In terms of citrus cultivation methods,the sod culture has become a hot spot.This paper focused on the benefits of sod culture in citrus orchards,the selection of grass species,and the cultivation techniques.It analyzed the problems that should be paid attention to in sod culture in citrus orchards,in order to provide a theoretical basis for production and cultivation of fruit farmers.展开更多
[Objectives]To screen the tissue culture rapid propagation formula suitable for each tissue culture stage of Anoectochilus roxburghii( Wall) Lind. [Methods] The stem segments of Fujian A. roxburghii were used as expla...[Objectives]To screen the tissue culture rapid propagation formula suitable for each tissue culture stage of Anoectochilus roxburghii( Wall) Lind. [Methods] The stem segments of Fujian A. roxburghii were used as explant to study the tissue culture rapid propagation and transplantation techniques. The comparative experiment was carried out to study the effects of different hormone concentrations on the induction of stem segments,proliferation of cluster buds,rooting and seedling hardening of A. roxburghii,and study the effects of transplantation matrix on the transplantation of A. roxburghii. [Results]MS + 0. 5 mg/L NAA + 2 mg/L BA + 20 g/L sucrose + 6 g/L agar was suitable for induction of stem segments of A. roxburghii; MS + 0. 5 mg/L NAA + 2 mg/L BA + 1 mg/L KT + 25 g/L sucrose + 6 g/L agar was most suitable for proliferation of cluster buds of A. roxburghii; MS + 1. 0 mg/L IBA + 1. 0 mg/L NAA + 1 g/L activated carbon + 50 g/L mashed banana + 25 g/L sucrose + 6 g/L agar was most suitable for rooting and seedling hardening of A. roxburghii; using peat soil: fine sand( 3∶ 1) as transplantation matrix,the survival rate was the highest. [Conclusions] The experiment results are expected to provide references for factory production of A. roxburghii.展开更多
To obtain the optimization formulation of pure cultures in Tibetan kefir,the influence of the different mixtures of five strains in the pure cultures in Tibetan kefir on the flavor components in fermented milk was stu...To obtain the optimization formulation of pure cultures in Tibetan kefir,the influence of the different mixtures of five strains in the pure cultures in Tibetan kefir on the flavor components in fermented milk was studied using the mixture design. The regression model on microorganism composition and main metabolites was established. The results suggested that the predictable production of lactate reached the maximum of 8.16 g L-1,while the most predictable production of diacetyl,ethanol,and CO2 were 77.23 mg L-1,4 259 mg L-1,and 2.12 g L-1,respectively. Based on these,the response values that satisfied all expectations were optimized,and the most excellent combination was Lactococcus lactis 27%,Leuconostoc mesenteroides 37%,Lactobacillus kefiri 11%,Lactobacillus casei 10%,and Kluyveromyces marxianus 15%. With the aid of analysis software (Design-expert 6.0.5),the formulation of pure cultures in Tibetan kefir can be optimized for several responses and the best formulation can be obtained.展开更多
Magnetotactic bacteria(MTB)display magnetotaxis ability because of biomineralization of intracellular nanometer-sized,membrane-bound organelles termed magnetosomes.Despite having been discovered more than half a centu...Magnetotactic bacteria(MTB)display magnetotaxis ability because of biomineralization of intracellular nanometer-sized,membrane-bound organelles termed magnetosomes.Despite having been discovered more than half a century,only a few representatives of MTB have been isolated and cultured in the laboratory.In this study,we report the genomic characterization of a novel marine magnetotactic spirillum strain SH-1 belonging to the genus Terasakiella that was recently isolated.A gene encoding haloalkane dehalogenase,which is involved in the degradation of chlorocyclohexane,chlorobenzene,chloroalkane,and chloroalkene,was identified.SH-1 genome contained cysCHI and soxBAZYX genes,thus potentially capable of assimilatory sulfate reduction to H_(2)S and using thiosulfate as electron donors and oxidizing it to sulfate.Genome of SH-1 also contained genes encoding periplasmic dissimilatory nitrate reductases(napAB),assimilatory nitrate reductase(nasA)and assimilatory nitrite reductases(nasB),suggesting that it is capable of gaining energy by converting nitrate to ammonia.The pure culture of Terasakiella sp.SH-1 together with its genomic results off ers new opportunities to examine biology,physiology,and biomineralization mechanisms of MTB.展开更多
Opera has been developed in China for more than a hundred years as an “imported product” in the West. But the deep-rooted traditional ideas and the artistic aesthetics accumulated over thousands of years have also g...Opera has been developed in China for more than a hundred years as an “imported product” in the West. But the deep-rooted traditional ideas and the artistic aesthetics accumulated over thousands of years have also given the opera a new youth in China. Among them, the most affected area is singing technique. As one of the most intuitive external performance features of opera, singing technique has been the most affected by “localization” and has created a unique singing method belonging to China. Of course, this method is not formed in one fell swoop. The purpose of this paper is to explore the process of “localization” of opera singing technique in the context of Chinese culture, and to demonstrate it completely from the forms, methods, ideas and systems of change, so as to apply the theory more deeply to practical singing and teaching.展开更多
为探究湖北襄阳地区不同颜色高温大曲中葡萄球菌(Staphylococcus)多样性,该研究通过下载并筛选葡萄球菌属相对含量>10%的样品序列,采用Illumina Mi Seq高通量测序技术和传统纯培养技术相结合的方法对大曲中葡萄球菌多样性进行解析。...为探究湖北襄阳地区不同颜色高温大曲中葡萄球菌(Staphylococcus)多样性,该研究通过下载并筛选葡萄球菌属相对含量>10%的样品序列,采用Illumina Mi Seq高通量测序技术和传统纯培养技术相结合的方法对大曲中葡萄球菌多样性进行解析。结果表明,高温大曲中葡萄球菌相对含量>10%的样品共有13份,包括7份黑色大曲、4份黄色大曲和2份白色大曲,葡萄球菌基因序列相对丰度占所有原核生物的14.33%~74.19%。多样性分析表明,黑色大曲中葡萄球菌丰富度和多样性均显著偏高(P<0.05),且不同颜色高温大曲中葡萄球菌群落结构差异显著(P<0.05)。操作分类单元(OTU)分析表明,高温大曲中共有OTU 5个,累积平均相对含量达到91.38%。传统纯培养技术表明,腐生葡萄球菌(Staphylococcus saprophyticus)是黑色、黄色和白色大曲中共有的可培养菌种,亦是高温大曲中葡萄球菌的主要可培养菌种。由此表明,黑色大曲中葡萄球菌丰富度和多样性更高,且腐生葡萄球菌为高温大曲中葡萄球菌的主要可培养菌种。展开更多
为探究湖北襄阳地区高温大曲中芽孢杆菌(Bacillus)菌群多样性,该研究通过下载并筛选高温大曲样品芽孢杆菌相对含量>2.0%的测序数据,采用Illumina Mi Seq高通量测序技术和传统纯培养技术相结合的方法对大曲样品中芽孢杆菌多样性进行...为探究湖北襄阳地区高温大曲中芽孢杆菌(Bacillus)菌群多样性,该研究通过下载并筛选高温大曲样品芽孢杆菌相对含量>2.0%的测序数据,采用Illumina Mi Seq高通量测序技术和传统纯培养技术相结合的方法对大曲样品中芽孢杆菌多样性进行解析。结果表明,高温大曲中芽孢杆菌相对含量>2.0%的样品共有12份,包括8份白色大曲样品和4份黄色大曲样品,所有样品共获得77 825条代表性序列,1 197个操作分类单元(OTUs)。多样性分析结果表明,较之黄色大曲,白色大曲样品中芽孢杆菌的超1指数和发现物种数显著偏高(P<0.05)。OTU分析结果表明,OTU5321和OTU5291在所有样品中均存在,其在白色大曲和黄色大曲中累积包含序列分别占总序列数的49.43%和41.32%。传统纯培养技术分析结果表明,解淀粉芽孢杆菌(Bacillus amyloliquefaciens)为高温大曲中芽孢杆菌的主要可培养菌种。由此可知,白色大曲芽孢杆菌群落结构的丰富度更高,且解淀粉芽孢杆菌为高温大曲中芽孢杆菌的主要可培养菌种,为后续高温大曲中芽孢杆菌的应用及菌种选育具有指导作用。展开更多
基金Supported by Science and Technology Project for Coordinating and Promoting Rural Revitalization in Sichuan Province (Industrial Development Category)"Key Technology Integration and Demonstration for Blood Orange-Soybean-Rapeseed’ Light,Simple and Efficient Compound Planting Mode (Park Project)"(2022ZHXC0009)Key Research and Development Project of Sichuan Province (Science and Technology Cooperation between Provincial Institutes and Universities)"Research on Key Technologies of Blood Orange and Lemon Compound Fermented Wine"(2023YFSY0054)
文摘Citrus is an important fruit in Sichuan,accounting for 52.6%fruit yield of the whole Sichuan Province.In recent years,citrus has expanded its original cultivation and planting area in Sichuan,and the planting area has continued to increase.In terms of citrus cultivation methods,the sod culture has become a hot spot.This paper focused on the benefits of sod culture in citrus orchards,the selection of grass species,and the cultivation techniques.It analyzed the problems that should be paid attention to in sod culture in citrus orchards,in order to provide a theoretical basis for production and cultivation of fruit farmers.
基金Supported by Science and Technology Project of Huizhou City(2014C040007001)
文摘[Objectives]To screen the tissue culture rapid propagation formula suitable for each tissue culture stage of Anoectochilus roxburghii( Wall) Lind. [Methods] The stem segments of Fujian A. roxburghii were used as explant to study the tissue culture rapid propagation and transplantation techniques. The comparative experiment was carried out to study the effects of different hormone concentrations on the induction of stem segments,proliferation of cluster buds,rooting and seedling hardening of A. roxburghii,and study the effects of transplantation matrix on the transplantation of A. roxburghii. [Results]MS + 0. 5 mg/L NAA + 2 mg/L BA + 20 g/L sucrose + 6 g/L agar was suitable for induction of stem segments of A. roxburghii; MS + 0. 5 mg/L NAA + 2 mg/L BA + 1 mg/L KT + 25 g/L sucrose + 6 g/L agar was most suitable for proliferation of cluster buds of A. roxburghii; MS + 1. 0 mg/L IBA + 1. 0 mg/L NAA + 1 g/L activated carbon + 50 g/L mashed banana + 25 g/L sucrose + 6 g/L agar was most suitable for rooting and seedling hardening of A. roxburghii; using peat soil: fine sand( 3∶ 1) as transplantation matrix,the survival rate was the highest. [Conclusions] The experiment results are expected to provide references for factory production of A. roxburghii.
文摘To obtain the optimization formulation of pure cultures in Tibetan kefir,the influence of the different mixtures of five strains in the pure cultures in Tibetan kefir on the flavor components in fermented milk was studied using the mixture design. The regression model on microorganism composition and main metabolites was established. The results suggested that the predictable production of lactate reached the maximum of 8.16 g L-1,while the most predictable production of diacetyl,ethanol,and CO2 were 77.23 mg L-1,4 259 mg L-1,and 2.12 g L-1,respectively. Based on these,the response values that satisfied all expectations were optimized,and the most excellent combination was Lactococcus lactis 27%,Leuconostoc mesenteroides 37%,Lactobacillus kefiri 11%,Lactobacillus casei 10%,and Kluyveromyces marxianus 15%. With the aid of analysis software (Design-expert 6.0.5),the formulation of pure cultures in Tibetan kefir can be optimized for several responses and the best formulation can be obtained.
基金Supported by the National Natural Science Foundation of China-Shandong Joint Fund(No.U1706208)the National Natural Science Foundation of China(Nos.41776131,41776130)。
文摘Magnetotactic bacteria(MTB)display magnetotaxis ability because of biomineralization of intracellular nanometer-sized,membrane-bound organelles termed magnetosomes.Despite having been discovered more than half a century,only a few representatives of MTB have been isolated and cultured in the laboratory.In this study,we report the genomic characterization of a novel marine magnetotactic spirillum strain SH-1 belonging to the genus Terasakiella that was recently isolated.A gene encoding haloalkane dehalogenase,which is involved in the degradation of chlorocyclohexane,chlorobenzene,chloroalkane,and chloroalkene,was identified.SH-1 genome contained cysCHI and soxBAZYX genes,thus potentially capable of assimilatory sulfate reduction to H_(2)S and using thiosulfate as electron donors and oxidizing it to sulfate.Genome of SH-1 also contained genes encoding periplasmic dissimilatory nitrate reductases(napAB),assimilatory nitrate reductase(nasA)and assimilatory nitrite reductases(nasB),suggesting that it is capable of gaining energy by converting nitrate to ammonia.The pure culture of Terasakiella sp.SH-1 together with its genomic results off ers new opportunities to examine biology,physiology,and biomineralization mechanisms of MTB.
文摘Opera has been developed in China for more than a hundred years as an “imported product” in the West. But the deep-rooted traditional ideas and the artistic aesthetics accumulated over thousands of years have also given the opera a new youth in China. Among them, the most affected area is singing technique. As one of the most intuitive external performance features of opera, singing technique has been the most affected by “localization” and has created a unique singing method belonging to China. Of course, this method is not formed in one fell swoop. The purpose of this paper is to explore the process of “localization” of opera singing technique in the context of Chinese culture, and to demonstrate it completely from the forms, methods, ideas and systems of change, so as to apply the theory more deeply to practical singing and teaching.
文摘为探究湖北襄阳地区不同颜色高温大曲中葡萄球菌(Staphylococcus)多样性,该研究通过下载并筛选葡萄球菌属相对含量>10%的样品序列,采用Illumina Mi Seq高通量测序技术和传统纯培养技术相结合的方法对大曲中葡萄球菌多样性进行解析。结果表明,高温大曲中葡萄球菌相对含量>10%的样品共有13份,包括7份黑色大曲、4份黄色大曲和2份白色大曲,葡萄球菌基因序列相对丰度占所有原核生物的14.33%~74.19%。多样性分析表明,黑色大曲中葡萄球菌丰富度和多样性均显著偏高(P<0.05),且不同颜色高温大曲中葡萄球菌群落结构差异显著(P<0.05)。操作分类单元(OTU)分析表明,高温大曲中共有OTU 5个,累积平均相对含量达到91.38%。传统纯培养技术表明,腐生葡萄球菌(Staphylococcus saprophyticus)是黑色、黄色和白色大曲中共有的可培养菌种,亦是高温大曲中葡萄球菌的主要可培养菌种。由此表明,黑色大曲中葡萄球菌丰富度和多样性更高,且腐生葡萄球菌为高温大曲中葡萄球菌的主要可培养菌种。
