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Transcriptome analysis reveals effects of red and blue lightemitting diodes(LEDs)on the growth,chlorophyll fluorescence and endogenous plant hormones of potato(Solanum tuberosum L.)plantlets cultured in vitro 被引量:4
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作者 CHEN Li-li WANG Hao-ying +3 位作者 GONG Xiao-chen ZENG Zhao-hai XUE Xu-zhang HU Yue-gao 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2021年第11期2914-2931,共18页
Red and blue light illumination has been reported to significantly affect plantlet growth.Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato ... Red and blue light illumination has been reported to significantly affect plantlet growth.Potato is an important food and feed crop in the world and potato plantlet cultured in vitro plays an important role in potato production.However,few studies have documented the effects of red and blue light on the growth of potato plantlets revealed at the transcriptome level.The objective of this study was to determine the growth and physiological responses of potato plantlets cultured in vitro under monochromatic red(RR),monochromatic blue(BB)as well as combined red and blue(RB)LEDs using the RNA-Seq technique.In total,3150 and 814 differentially expressed genes(DEGs)were detected in potato plantlets under RR and BB,respectively,compared to RB(used as control).Compared to the control,the DEGs enriched in"photosynthesis"and"photosynthesis-antenna proteins"metabolic pathways were up-regulated and down-regulated by BB and RR,respectively,which might be responsible for the increases and decreases of maximum quantum yield(F_(v)/F_(m)),photochemical quantum yield(φ_(PSII)),photochemical quenching(q_(P))and electron transfer rate(ETR)in BB and RR,respectively.Potato plantlets exhibited dwarfed stems and extended leaves under BB,whereas elongated stems and small leaves were induced under RR.These dramatically altered plantlet phenotypes were associated with variable levels of endogenous plant hormones gibberellin(GAs),indoleacetic acid(IAA)and cytokinins(CKs),as assessed in stems and leaves of potato plantlets.In addition,monochromatic red and blue LEDs trigged the opposite expression profiles of DEGs identified in the"plant hormone signal transduction"metabolic pathway,which were closely related to the endogenous plant hormone levels in potato plantlets.Our results provide insights into the responses of potato plantlets cultured in vitro to red and blue LEDs at the transcriptomic level and may contribute to improvements in the micro-propagation of potato plantlets cultured in vitro from the light spectrum aspect. 展开更多
关键词 potato(solanum tuberosum L.)plantlets in vitro red/blue LEDs light sources RNA-seq chlorophyll fluorescence plant hormone
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Preliminary Findings on Yield Response of Potatoes (Solanum tuberosum) to Umostart Super Zn (11.46,0 + 2 Zn + Humates)
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作者 C. Lung' aho M. Nyongesa M. Wakahiu 《Journal of Agricultural Science and Technology(B)》 2011年第5期728-734,共7页
Field trials to determine the yield response of potatoes to Umostart Super Zn (USZ) fertilizer (11.46.0 + 2 Zn + Humates) were carried out in 2004 using the cultivar Tigoni. During the long rains season, the tre... Field trials to determine the yield response of potatoes to Umostart Super Zn (USZ) fertilizer (11.46.0 + 2 Zn + Humates) were carried out in 2004 using the cultivar Tigoni. During the long rains season, the treatments included USZ at 15 kg ha^-1 at planting; 30 kg ha^-1 split into 15 kg ha^-1 at planting and 15 kg ha^-1 during final earthing-up; 30 kg ha^-1 at planting; and 45 kg ha^-1 at planting. Treatments during the short rains included USZ at 30 kg ha^-1 split into 15 kg ha^-1 at planting and 15 kg ha^-1 during final earthing-up; 30 kg ha^-1 at planting; 40 kg ha^-1 at planting; 50 kg ha^-1 at planting; 50 kg ha^-1 split into 25 kg ha^-1 at planting and 25 kg ha^-1 during final earthing-up; and 60 kg ha^-1 at planting. USZ was contrasted with Diammonium phosphate (DAP) fertilizer (18:46:0) at 300 kg ha^-1 and 500 kg ha^-1 applied at planting and a control in which no fertilizer was applied. The DAP 300 kg ha^-1 treatment was however, not included during the long rains season. The highest tuber yields among the USZ treatments were obtained in plots treated with 45 kg ha^-1 (27.11 t ha^-1) during the long rains and 60 kg ha^-1 (55.17 t ha^-1) during the short rains season. Application of USZ resulted in a yield increase of at least 3.90 and 19.06 t ha^-1 during the long and short rains seasons respectively. 展开更多
关键词 Fertilizers potato solanum tuberosum tuber yield Umostart Super Zn
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Impact of Organic Soil Amendments on the Physical Characteristics and Yield Components of Potato (Solanum tuberosurn L.) in the Highlands of Cameroon 被引量:1
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作者 Takoutsing Bertin Asaah Ebenezar +3 位作者 Yuh Renata Tchoundjeu Zacharie Degrande Ann Kouodiekong Lazare 《Journal of Agricultural Science and Technology(A)》 2013年第4期257-266,共10页
A field experiment was conducted in the Western Highlands of Cameroon to study the effects of Calliandra calothyrsus, sterilized compost, non-sterilized compost and mineral fertilizers (NPK 11:11:22) on physical c... A field experiment was conducted in the Western Highlands of Cameroon to study the effects of Calliandra calothyrsus, sterilized compost, non-sterilized compost and mineral fertilizers (NPK 11:11:22) on physical characteristics, yield components and late blight disease severity of potato (Solanum tuberosum). A two factorial treatment combination made up of fertilization schemes and sanitary measures were laid out in a randomized complete block design (RCBD) with four replicates and 10 treatments. Data collected were subjected to a multivariate ANOVA and means separated with the Dunnett t-test with Calliandra calothyrsus considered as the main treatment. Mineral fertilizers and Calliandra calothyrsus significantly augmented stem diameter, plant height and plant vigor, total and marketable yields, despite the fact that late blight severity was high in the two treatments. The correlation matrix showed that total yield had significant and positive correlation with stem diameter (r = 0.74), plant height (r = 0.61), plant vigor (r = 0.61) and marketable yield (r = 0.99) and negative correlation (r = -37) with late blight severity. The increase observed is attributed to the nutrient contents of the treatments applied. Leafy prunings of C. calothyrsus increase soil organic matter which probably improves moisture retention and biological activity. The highest late blight severity recorded between the 45 and 60 coincides with active development of plant canopy which influences disease severity and create a canopy microclimate conducive to disease development. This study suggests that Calliandra calothyrsus has the potential of improving potato production provided appropriate fungicide is used. 展开更多
关键词 Calliandra calothyrsus late blight severity organic manure mineral fertilizers COMPOST potato solanum tuberosum L.).
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马铃薯DHN基因家族的鉴定与分析
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作者 张宴萍 魏延政 +3 位作者 董道峰 胡柏耿 赵朋 杨晓慧 《山东农业科学》 北大核心 2024年第10期18-25,共8页
脱水素(dehydrin,DHN)作为一种高亲水性蛋白,在植物响应各种生物、非生物胁迫过程中发挥重要作用。本研究在马铃薯基因组中鉴定出7个StDHNs基因,不均匀地分布在3条染色体上,编码氨基酸数目为80~243 aa,内含子数目均为1;StDHNs蛋白分子量... 脱水素(dehydrin,DHN)作为一种高亲水性蛋白,在植物响应各种生物、非生物胁迫过程中发挥重要作用。本研究在马铃薯基因组中鉴定出7个StDHNs基因,不均匀地分布在3条染色体上,编码氨基酸数目为80~243 aa,内含子数目均为1;StDHNs蛋白分子量在8.54~25.12 kDa之间,等电点为5.24~7.38,共3个保守motifs,保守基序及进化关系分析表明StDHN家族成员在进化上具有高度的保守性。转录表达分析结果显示,StDHN5、StDHN6、StDHN7在特定逆境胁迫下显著高表达。诱导表达模式分析表明,StDHN5、StDHN6受到盐和ABA胁迫的显著诱导,StDHN7响应干旱胁迫。本研究结果可为深入研究马铃薯DHN基因家族的功能及其在逆境胁迫抗性中的应用提供科学依据。 展开更多
关键词 马铃薯 DHN基因家族 生物信息学分析 表达分析
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马铃薯种质资源抗黑痣病鉴定及SSR聚类分析
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作者 王紫雯 金光辉 +3 位作者 王腾 王鹏程 高睿 吴国强 《黑龙江八一农垦大学学报》 2024年第5期15-23,共9页
马铃薯黑痣病已成为影响马铃薯产量和商品价值的重要土传病害。为丰富马铃薯抗黑痣病种质资源,提高抗黑痣病育种工作效率,研究通过54份马铃薯种质资源地下茎与匍匐茎黑痣病抗性评价,共筛选出7份中抗资源,分别为克17-33-270、龙18-124-8... 马铃薯黑痣病已成为影响马铃薯产量和商品价值的重要土传病害。为丰富马铃薯抗黑痣病种质资源,提高抗黑痣病育种工作效率,研究通过54份马铃薯种质资源地下茎与匍匐茎黑痣病抗性评价,共筛选出7份中抗资源,分别为克17-33-270、龙18-124-8、龙18-7-100、ND11-7-22、克18-134-5、克17-115-1、克17-6-9。研究利用8对SSR适宜引物对7份抗性材料DNA进行PCR扩增,获得SSR多态性条带42条,多态性比率89.36%,遗传相似性系数在0.444 4~0.833 3之间,以相似性系数0.62为基准,可将7份抗性材料分为3类,其中克18-134-5和克17-115-1为一类;龙18-124-8和龙18-7-100为一类;克17-33-270、ND11-7-22和克17-6-9为一类。 展开更多
关键词 马铃薯(solanum tuberosum L.) 种质资源 抗性鉴定 黑痣病 SSR分子标记
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马铃薯根系分泌物对马铃薯金线虫孵化和趋化的影响及外源酸类物质的验证
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作者 尹艳蝶 杨艳梅 +6 位作者 付启春 王琴 李永青 段锦凤 刘玉竹 王桥美 胡先奇 《中国农业科学》 CAS CSCD 北大核心 2024年第11期2161-2175,共15页
【目的】探究马铃薯不同抗性品种、不同生育期根系分泌物对马铃薯金线虫(Globodera rostochiensis)孵化和趋化的影响,为马铃薯金线虫绿色防控提供理论依据。【方法】收集4个马铃薯品种3个生育期的根系分泌物,采用浸泡法和滤纸片法研究... 【目的】探究马铃薯不同抗性品种、不同生育期根系分泌物对马铃薯金线虫(Globodera rostochiensis)孵化和趋化的影响,为马铃薯金线虫绿色防控提供理论依据。【方法】收集4个马铃薯品种3个生育期的根系分泌物,采用浸泡法和滤纸片法研究根系分泌物对马铃薯金线虫孵化和趋化的影响;通过GC-MS分析各根系分泌物的组分;选用8种外源酸类物质咖啡酸、丁二酸、氢化肉桂酸、3-羟基丙酸、3-氨基-3-甲基丁酸、乙醇酸、硬脂酸、L-乳酸的标准品,运用浸泡法和滤纸片法验证根系分泌物中占比较大的主要酸类物质对马铃薯金线虫孵化和趋化的影响。【结果】感病品种青薯9号、会-2的根系分泌物比抗病品种宣薯6号、云薯505的根系分泌物刺激孵化快且孵化量大,在3个生育期(15 d(发芽期)、30 d(幼苗期)和55 d(块茎形成期)),感病品种根系分泌物的刺激孵化效果强于抗病品种,各品种30 d(幼苗期)的根系分泌物刺激孵化效果好于15 d(发芽期)、55 d(块茎形成期),青薯9号根系分泌物的刺激孵化率15、30、55 d依次为7.20%、27.30%、5.70%,会-2依次为10.50%、38.00%、9.40%,宣薯6号依次为5.40%、20.40%、16.30%,云薯505依次为13.20%、21.80%、3.10%。感病品种青薯9号的根系分泌物对马铃薯金线虫幼虫的吸引能力强;从3个生育期看,除了宣薯6号,其余3个品种15 d根系分泌物的吸引效果好于30、55 d,15、30、55 d的根系分泌物吸引率青薯9号依次为13.73%、9.68%、6.13%,会-2依次为5.65%、4.76%、4.89%,云薯505依次为4.86%、4.13%、2.91%,宣薯6号依次为4.11%、5.77%、6.47%。用GC-MS对根系分泌物进行分析鉴定,马铃薯根系分泌物中主要含有9类物质,其中酸类24种、氨基酸类13种、盐类5种、醇类3种、烃类3种、糖类3种、酚类2种、酮类1种、脂类1种,从种类和所占比例可知酸类、氨基酸类、酚类和醇类是供试马铃薯根系分泌物中的主要成分。选择8种代表性酸类物质验证表明,8种物质中丁二酸、3-羟基丙酸对马铃薯金线虫的孵化有显著影响,咖啡酸、氢化肉桂酸对马铃薯金线虫的趋化有显著影响。但与4个品种30 d根系分泌物对马铃薯金线虫的影响对比,上述影响显著的酸类物质刺激孵化效果显著低于根系分泌物,趋化则无显著差异。【结论】不同马铃薯品种的根系分泌物均对马铃薯金线虫孵化和趋化有较大影响,感病品种根系分泌物对马铃薯金线虫孵化、趋化的影响强于抗病品种,总体表现为抗病、感病品种对马铃薯金线虫的影响在根系出现时即播种15 d(发芽期)开始,在30 d(幼苗期)影响最强,到55 d(块茎形成期)影响减弱。马铃薯的根系分泌物种类多样,主要组分为酸类、氨基酸类和醇类,抗病、感病品种略有差异。丁二酸、3-羟基丙酸对孵化的影响显著,咖啡酸、氢化肉桂酸对趋化的影响显著,单一酸性物质对马铃薯金线虫孵化的影响显著小于根系分泌物。 展开更多
关键词 马铃薯 根系分泌物 马铃薯金线虫 孵化 趋化 组分
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Classification and Integration of Storage and Transportation Engineering Technologies in Potato Producing Areas of China
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作者 孙洁 王希卓 +3 位作者 黄振霖 孙海亭 程勤阳 朱明 《Agricultural Science & Technology》 CAS 2017年第4期710-718,共9页
Considering the development of potato (Solanum tuberosum) industry in China, the existing technologies of potato storage and transportation in the produc- ing area were analyzed through investigation on four main po... Considering the development of potato (Solanum tuberosum) industry in China, the existing technologies of potato storage and transportation in the produc- ing area were analyzed through investigation on four main potato production areas. Unear classification was used to conduct the technology classification. According to the technical attributes and characteristics, the potato technologies of storage and transportation in producing area were classified with large classes, middle classes, small classes and subclasses, into the agricultural production area processing and storage engineering technology system, to reveal the structure and functions. Mean- while, the widely used technologies were integrated and summarized into 5 principal technology integration programs, which could be used for the technology integration of the new management subjects such as planting professional cooperatives, family farms, enterprises and so on. 展开更多
关键词 potato solanum tuberosum Storage and transportation in producing area Technology classification Technology integration
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The Key Factors Affecting Tuber Development of Potato in vitro and the Relation with Protein Fractions 被引量:1
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作者 WANG Da-yong, LIAN Yong and ZHU De-wei( Institute of Vegetables and Flowers , Chinese Academy of Agricultural Sciences , Beijing 100081 ) 《Agricultural Sciences in China》 CAS CSCD 2002年第3期256-264,共9页
According to previous analysis, some properties bounding up with tuber yield were investigated. The results showed that tuber average weight, plastid Mg2 + -ATPase activity, plastid Ca2 + -ATPase activity, mitochondri... According to previous analysis, some properties bounding up with tuber yield were investigated. The results showed that tuber average weight, plastid Mg2 + -ATPase activity, plastid Ca2 + -ATPase activity, mitochondria Mg2 + -ATPase activity, total soluble protein content, tuber average diameter, and Q-enzyme activity were important factors determining the tuber yield. The linear regression equation was:Y = 0.5211 + 0.0595X(1)+0.8389X(2) +0.0882X(3) -0.0073X(4) +0.1449X(5) +0.3510X(6) +0.0031X(7) -0.00003X(8) + 0.3412X(9) + 0.0127X(10) + 0.2904X(ll) + 0.0570X(12) + 0.0159X(13) + 0.3585X(14) + 0.0134X(15) - 0.1012X(16). At the same time, the relation between several important properties and soluble protein fractions were analyzed. 展开更多
关键词 potato (solanum tuberosum L.) In vitro culture TUBER Soluble protein Multivariate statistics
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The Impact of High Temperature during Growing Season on Potato Cultivars with Different Response to Environmental Stresses 被引量:1
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作者 Krystyna Rykaczewska 《American Journal of Plant Sciences》 2013年第12期2386-2393,共8页
Potato crop is the fourth main food crops in the world after maize, rice and wheat. It is characterized by specific temperature requirements and develops best at about 20°C. Forecasts of global warming prompt us ... Potato crop is the fourth main food crops in the world after maize, rice and wheat. It is characterized by specific temperature requirements and develops best at about 20°C. Forecasts of global warming prompt us to study the tolerance of potato genotypes to heat during the growing season. The aim of this work was to assess the response of chosen potato cultivars to high temperature during the different stages of plant growth under conditions of good soil moisture and drought. The impact of high temperature 32°C/25°C on potato plants was determined in pot experiment in three growth stages. A main measure of tolerance of the potato cultivars to high temperature during the growing season was an evaluation of the yield in relation to the Control combination. Here we demonstrated that tested potato cultivar’s response to high temperature during the growing season is dependent on the growth stage. The earlier it occurs, the more negative its impact on the growth and yield of potatoes is. 展开更多
关键词 potato (solanum tuberosum L) Heat Stress DURING Growing Period Drought TUBER Yield Physiological Defects of TUBERS
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The Role of Biodiversity in Food Security and Nutrition: A Potato Cultivar Case Study 被引量:1
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作者 Carmen van Niekerk Hettie Schönfeldt +1 位作者 Nicolette Hall Beulah Pretorius 《Food and Nutrition Sciences》 2016年第5期371-382,共12页
Biodiversity is considered a critical measure of the agricultural health of the world. Not only does increased biodiversity contribute to nutrient production and consumption, but it acts as a safeguard against food sh... Biodiversity is considered a critical measure of the agricultural health of the world. Not only does increased biodiversity contribute to nutrient production and consumption, but it acts as a safeguard against food shortages due to pests and diseases by spreading the risk. Biodiversity can improve dietary diversity in such a way to ultimately contribute to improved food and nutrition security. As a result biodiversity is often highlighted in global discussions related to food and nutrition security. Potatoes (Solanum tuberosum L.) are highly biodiverse food crops, with more than 4000 different cultivars grown globally. The crop is sometimes considered as part of the vegetable component of food baskets, but it is mainly added to meals as a starch because potatoes have a high starch content and are thus high in energy. However, significant differences in the nutritional content (including micronutrient composition) of different potato cultivars have been reported in many countries. It is therefore proposed that specific cultivars could potentially contribute more to critical nutrients required in the diet of countries at risk of malnutrition. The nutritional profile (macronutrients and minerals) of 11 potato cultivars cultivated in sub-Saharan Africa was determined and the contribution which these different potatoes could make to the diet, and consequently to food and nutrition security, is discussed. The results showed that significant differences are found in the nutritional content between the different cultivars. Significant differences (p < 0.001) were found in macronutrients such as protein and fat as well as micronutrients such as copper, phosphorus and potassium. These results indicate the potentially beneficial role which a biodiverse range of crops such as potatoes, could play in the food and nutrition security of developing countries. 展开更多
关键词 potatoES solanum tuberosum Nutrient Content BIODIVERSITY Sub-Saharan Food and Nutrition Security
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Genetic Variance Estimates in Heterogeneous Potato Populations Propagated from True Potato Seed(TPS) 被引量:1
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作者 M K Biswas M A A Mondal +1 位作者 M Hossain R Islam 《中国马铃薯》 2005年第2期65-69,共5页
Twenty nine cross populations developed according to line × tester mating design along with their twelve parents raised from true potato seeds, were used to study variability and correlation for seven characters.... Twenty nine cross populations developed according to line × tester mating design along with their twelve parents raised from true potato seeds, were used to study variability and correlation for seven characters. The range of variation for all the characters were found to be wide and pronounced which indicated that characters were quantitative in nature and were under polygenic control. For all the characters, greater portion of the total δ2p was mostly contributed by the δ2g. Among the seven characters, tuber weight per plant showed the highest δ2g, PCV, GCV, H2b, GA and expected GA%. On the other hand, dry matter contents (%) showed the lowest H2b. Phenotypic and genotypic correlation coefficients of plant height with no. of tubers per plant and tuber weight per plant; no. of branches per plant with no. of tubers per plant and tuber weight per plant were found to be significant and positive. 展开更多
关键词 马铃薯 遗传变异 评估 种子繁殖 相关性
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Effects of Agrobacterial<i>rol</i>-Genes on the Thermodynamic and Structural Features of Starches Extracted from Potato Microtubers
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作者 Luybov A. Wasserman Nina P. Aksenova +4 位作者 Tatiyana N. Konstantinova Lidiya I. Sergeeva Svetlana A. Golyanovskaya Alexey V. Krivandin Georgy A. Romanov 《Food and Nutrition Sciences》 2014年第3期250-257,共8页
Wild-type potato (Solanum tuberosum L.) plants and their transformants harboring agrobacterial rolB or rolC genes under control of the patatin class I promoter were cultured in vitro. These plants were used as a sourc... Wild-type potato (Solanum tuberosum L.) plants and their transformants harboring agrobacterial rolB or rolC genes under control of the patatin class I promoter were cultured in vitro. These plants were used as a source of single-node stem cuttings. The structure of native starch in tubers formed on cuttings was determined using methods of X-ray scattering and differential scanning microcalorimetry (DSC). It was found that in starch from tubers of rolB plants the melting temperature of crystalline lamella was lower and their thickness was less than that in wild-type potato. In tubers of rolC plants starch differed from starch in wild-type plants by a higher melting temperature, reduced melting enthalpy, and a greater thickness of crystalline lamellae. The melting of starch from tubers of rolC plants proceeded as the melting of two independent crystalline structures with melting temperatures of 338.0°K and 342.8°K. Overall data show that starches of different structure can be obtained by using transgenic approach. 展开更多
关键词 solanum tuberosum Transgenic potato Starch ROL GENES TUBERS Crystalline LAMELLAE Melting Temperature
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Analysis of Diversity among Potato Accessions Grown in Eritrea Using Single Linkage Clustering
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作者 Biniam Mesfin Ghebreslassie S. Mwangi Githiri +1 位作者 Tadesse Mehari Remmy W. Kasili 《American Journal of Plant Sciences》 2015年第13期2122-2127,共6页
Potato is an important cash and food crop, a source of income to many small scale farmers in Eritrea. In the long tradition of potato cultivation farmers have adopted a number of landraces. However, very little is kno... Potato is an important cash and food crop, a source of income to many small scale farmers in Eritrea. In the long tradition of potato cultivation farmers have adopted a number of landraces. However, very little is known about their genetic diversity which limits further breeding programs to improve productivity. The objective of the study was to characterize potato accessions grown in Eritrea. A total of 21 accessions collected from farmers and NARI were used in this study for characterization employing 33 phenotypic descriptors. Single linkage (nearest neighbor) clustering method using UPGMA employing Euclidean test was used to yield a dendrogram. The clustering generated three main clusters with further sub clusters. The clustering helped to identify accessions that shared the same characteristics and/or that were closely related and vice versa. It was noted that there was no distinct relationship between the clusters groups and geographic sources of accession. Yet, accessions sharing the same name may not necessarily share the same genetic content. Mean deviation of variables for each group from the total mean indicated that Group III followed by group I showed negative deviation in most of the recorded yield related traits. Group I was particularly affected by the presence of two inferior accessions in the group. On the other hand, Group II accessions showed relatively maximum deviation from the total mean and could be recommended for use as parent for any breeding program in Eritrea. 展开更多
关键词 solanum tuberosum potato ERITREA Morphological Traits Cluster DIVERSITY
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Effects of different planting systems on yield of potato crop in Kaghan Valley: A mountainous region of Pakistan
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作者 Muhammad Qasim Salma Khalid +2 位作者 Alia Naz Muhammad Zafarullah Khan Sohail Ahmad Khan 《Agricultural Sciences》 2013年第4期175-179,共5页
Conversion of potato from conventional methods to wide bed planting systems may increase water and nitrogen use efficiency in commercial potato production system by reducing the amount of irrigation water and water ap... Conversion of potato from conventional methods to wide bed planting systems may increase water and nitrogen use efficiency in commercial potato production system by reducing the amount of irrigation water and water applied nitrogen fertilizer bypassing the potato root zone. Potato (Solanum tuberosum L) cv. Desiree was tested against different planting system for yield and yield components at Kaghan, a high mountainous Himalayan region ofPakistan. The experiment was carried out at Himalayan Agricultural Research Station (HARS), Kaghan during the summer season of 2005. The results showed that maximum tuber growth (88.7%), number of stems per plant (3.5), plant expansion (45.5 cm), average number of tubers per plant (10.1) and yield per hectare (12.4 t/ha) were significantly different and higher when potatoes were planted on wide bed and covered with soil from one side. Tallest plants (53.4 cm) were observed when potatoes were sown on the ridges. Maximum number of green potatoes (12.5) and injured potatoes (5.3%) were observed when the tubers were planted following local farmers’ method. Keeping in view the soil type, land slopping, we recommend sowing potatoes on relatively plain wide beds and covering it with soil from one side, for potato cultivation in the area. 展开更多
关键词 potato (solanum tuberosum L) PLANTING Systems YIELD Response Kaghan VALLEY Pakistan
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马铃薯低氮肥胁迫响应TCP转录因子的鉴定与分析 被引量:2
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作者 牛苏燕 梁芳 +5 位作者 张珍华 蒋素华 武思 王默霏 袁秀云 崔波 《广西植物》 CAS CSCD 北大核心 2023年第2期293-302,共10页
TCP转录因子是植物特有的一类转录因子,参与植物生物学过程的多个方面。为研究马铃薯TCP转录因子在响应低氮肥胁迫中的作用,该研究以氮肥供应不足(0.05 mmol·L^(-1))和氮肥供应充足(7.5 mmol·L^(-1))条件下马铃薯的根和叶片构... TCP转录因子是植物特有的一类转录因子,参与植物生物学过程的多个方面。为研究马铃薯TCP转录因子在响应低氮肥胁迫中的作用,该研究以氮肥供应不足(0.05 mmol·L^(-1))和氮肥供应充足(7.5 mmol·L^(-1))条件下马铃薯的根和叶片构建4个转录组文库进行测序,并对差异表达的TCP转录因子进行分析。结果表明:(1)在4个转录组文库中共鉴定TCP转录因子24个,它们主要分布在2号、3号、6号染色体上。(2)经结构域分析显示,24个TCP转录因子均具有典型的basic-Helix-Loop-Helix结构域。(3)经系统进化分析显示,马铃薯与拟南芥TCP蛋白可聚集在一起,分属于10个亚类。(4)转录组测序结果显示,在低氮肥胁迫下,大多数TCP转录因子被抑制表达,有3个TCP转录因子在根中显著性差异表达,5个TCP转录因子在叶中特异性表达。(5)根据GO功能注释分析和马铃薯TCP转录因子与拟南芥TCP转录因子的亲缘关系分析推测,这些TCP转录因子参与了马铃薯对低氮肥胁迫的响应。该研究结果为进一步研究马铃薯与其他粮食作物TCP转录因子响应低氮肥胁迫的分子功能奠定了基础。 展开更多
关键词 马铃薯 TCP转录因子 氮肥胁迫 转录组测序 生物信息学分析
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马铃薯植株株型构成要素解析 被引量:2
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作者 李丽 周晓洁 +3 位作者 袁剑龙 程李香 郭仁世 张峰 《核农学报》 CAS CSCD 北大核心 2023年第9期1764-1774,共11页
马铃薯植株的理想株型是获得高质量群体的基础。为获得理想株型构成要素参数,本研究对26个马铃薯品种(系)进行产量组分分析,选择中高产品种(系)测定植株茎、叶相关株型性状,结合方差分析、相关性分析和灰色关联分析确定理想株型构成要... 马铃薯植株的理想株型是获得高质量群体的基础。为获得理想株型构成要素参数,本研究对26个马铃薯品种(系)进行产量组分分析,选择中高产品种(系)测定植株茎、叶相关株型性状,结合方差分析、相关性分析和灰色关联分析确定理想株型构成要素及其参数。聚类分析将26个马铃薯品种(系)划分为超高产、高产、中产、中低产和低产五种类型。中高产品种(系)平均主茎数为2.29~2.89;三种产量类型中,超高产品种(系)自然株高均值最小,高产品种(系)最大;超高产品种(系)直立性E值最大,中产品种(系)最小;超高产品种(系)基部第4、第5、第7、第8节间长度均最小,基部第4到第8节间直径均最大,基部第3、第4、第5、第7茎叶夹角均最小。除超高产品种G6和高产品种G4,其余品种(系)叶片均属于疏散型,小叶为7或9个,马铃薯羽状复叶在主茎上螺旋上升排列,66.67%的品种(系)同一螺旋轨迹相邻羽状复叶水平相差60°。理想株型的主要构成要素包括幼苗期至块茎成熟期叶面积指数、基部第3至第7茎叶夹角、基部第4至第8节间直径、羽状复叶数量及长度和绝对株高。理想株型的次要构成要素为主茎数和自然株高。本研究解析的高产马铃薯品种(系)株型参数为田间高效管理和株型育种提供了理论基础。 展开更多
关键词 马铃薯 植株株型 理想株型 产量组分
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马铃薯StCYP83基因家族鉴定及其抗晚疫病的功能分析 被引量:2
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作者 孔乐辉 宗德乾 +5 位作者 史青尧 殷盼盼 巫文玉 田鹏 单卫星 强晓玉 《中国农业科学》 CAS CSCD 北大核心 2023年第16期3124-3139,共16页
【目的】通过鉴定马铃薯StCYP83基因家族成员,分析其响应致病疫霉(Phytophthora infestans)侵染的表达模式,挖掘具有抗晚疫病功能的StCYP83并解析其免疫机制,为马铃薯抗晚疫病分子育种提供新型抗性基因资源。【方法】利用双向BLAST法鉴... 【目的】通过鉴定马铃薯StCYP83基因家族成员,分析其响应致病疫霉(Phytophthora infestans)侵染的表达模式,挖掘具有抗晚疫病功能的StCYP83并解析其免疫机制,为马铃薯抗晚疫病分子育种提供新型抗性基因资源。【方法】利用双向BLAST法鉴定StCYP83基因家族成员,通过ExPASy Prot Param、Cell-Ploc 2.0、ESPript等软件分析StCYP83蛋白序列基本信息、亚细胞定位情况、保守基序;利用qRT-PCR技术分析StCYP83响应致病疫霉侵染的表达模式;利用农杆菌介导的本氏烟瞬时表达体系和马铃薯过表达(OE)稳定转化植株分析候选基因影响寄主植物抗致病疫霉的免疫功能及作用机理。【结果】在马铃薯基因组中共鉴定到10个StCYP83家族基因,分别命名为StCYP83B1—StCYP83B10,编码蛋白长度介于387—503aa,分子量介于44—57 kDa,亚细胞定位预测结果显示StCYP83蛋白均定位于内质网膜。qRT-PCR结果表明,StCYP83家族成员在不同程度上均可响应致病疫霉侵染而诱导表达,暗示StCYP83家族基因可能在马铃薯与致病疫霉互作中发挥作用。基于此,从中选取明显响应致病疫霉侵染而诱导上调表达、且与拟南芥AtCYP83B1同源性最高的StCYP83B1用于后续的免疫功能解析。本氏烟瞬时过表达的接菌测试结果表明,StCYP83B1具有抗致病疫霉的生物学功能;同时,过表达StCYP83B1可显著促进PTI标记基因(NbWRKY7、NbWRKY8)、SA信号通路标记基因(NbPR1和NbPR2)和JA信号通路标记基因(NbPR3和NbLOX)的上调表达,并提高flg22诱发的活性氧迸发。此外,StCYP83B1编码蛋白保守基序中的半胱氨酸位点为其抗性功能所必需。StCYP83B1过表达(StCYP83B1-OE)株系对致病疫霉的抗性有所增强,且呈现出增强的PTI免疫反应,包括flg22诱发的活性氧水平升高以及PTI标记基因(StWRKY7、StWRKY8和StACRE31)的显著诱导上调表达。此外,马铃薯SA信号通路相关基因(StPR1、StPR2、StPR5和StPAL2)和JA信号通路相关基因(StLOX、StAOS和StOPR3)也被诱导上调表达。【结论】共鉴定到10个StCYP83家族成员,StCYP83家族成员在不同程度上均可响应致病疫霉的侵染而诱导表达。StCYP83B1通过激活PTI、SA和JA信号通路调控植物对致病疫霉的抗性;而StCYP83B1血红素结合域中的半胱氨酸位点为其抗性功能所必需。 展开更多
关键词 马铃薯 StCYP83基因家族 致病疫霉 植物抗病性 晚疫病
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半干旱区膜下滴灌量与滴灌次数对马铃薯生长及水分利用效率影响 被引量:3
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作者 贾莉 陈娟 +2 位作者 雍山玉 李效文 梁伟琴 《中国农业科技导报》 CAS CSCD 北大核心 2023年第5期176-184,共9页
为实现半干旱区马铃薯节水增产,确定适宜的灌水量与灌水次数,在滴灌条件下,以‘冀张薯12号’马铃薯为供试材料,采用随机区组设计,以覆膜无灌水作为对照(CK),分别灌水600(W1)、1200(W2)、1800 m^(3)·hm^(-2)(W3)及灌水2(F1)、3(F2)... 为实现半干旱区马铃薯节水增产,确定适宜的灌水量与灌水次数,在滴灌条件下,以‘冀张薯12号’马铃薯为供试材料,采用随机区组设计,以覆膜无灌水作为对照(CK),分别灌水600(W1)、1200(W2)、1800 m^(3)·hm^(-2)(W3)及灌水2(F1)、3(F2)、4次(F3),共计10个处理,分析不同灌水量与灌水次数对马铃薯生长、产量、水分利用效率及经济效益的影响。结果表明,马铃薯的株高、叶面积指数、干物质累积量、SPAD值均随着生育期的推进先增后减。与CK相比,滴灌能显著促进马铃薯生长,提高地上部干物质累积量,以W2F3处理的生长指标最高。马铃薯耗水量和产量均随着滴灌量和灌水次数的增加而增加;水分利用效率随着滴灌量的增加先升高后降低。其中W2F3处理的水分利用效率和纯收益最高,分别为102.30 kg·hm^(-2)·mm-1和24203.88元·hm^(-2),较CK分别显著增加49.63%和117.19%。由此表明,适宜的灌水量和合理的灌水次数有利于促进马铃薯生长,提高产量并获得较高的经济收益,为该区马铃薯滴灌技术的推广与应用提供了理论支撑和科学指导。 展开更多
关键词 马铃薯 膜下滴灌 灌水次数 水分利用效率 纯收益
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不同马铃薯品种产量及营养品质分析与评价
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作者 朱杰 温海霞 +5 位作者 方治国 宿秀丽 张杰 吴峰 李晓敏 王文建 《湖北农业科学》 2023年第12期33-37,68,共6页
为筛选出优质高产马铃薯(Solanum tuberosum L.)新品种,采用等距分级法对8个参试马铃薯品种进行产量和营养品质综合分析与评价。结果表明,中薯3号营养品质最优,华薯16号产量最高,中薯685综合指数最高。综合产量和营养品质来看,中薯685... 为筛选出优质高产马铃薯(Solanum tuberosum L.)新品种,采用等距分级法对8个参试马铃薯品种进行产量和营养品质综合分析与评价。结果表明,中薯3号营养品质最优,华薯16号产量最高,中薯685综合指数最高。综合产量和营养品质来看,中薯685为最优品种,其次是华薯16号。 展开更多
关键词 马铃薯(solanum tuberosum L.) 品种筛选 产量 营养品质 等距分级
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冷凉灌区马铃薯施肥量与种植密度对马铃薯生长及产量的影响 被引量:1
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作者 王平 谢成俊 +3 位作者 孙振荣 陈娟 王镭 彭文静 《农学学报》 2023年第6期17-24,共8页
为了明确不同施肥量(复合肥)和种植密度对冷凉灌区马铃薯生长的影响。试验以‘冀张薯12号’为供试品种,开展施肥量与种植密度二因素试验,设3个施肥量(600、1100、1600 kg/hm^(2))与4个种植密度(5.25万、6万、6.75万、7.5万株/hm^(2)),... 为了明确不同施肥量(复合肥)和种植密度对冷凉灌区马铃薯生长的影响。试验以‘冀张薯12号’为供试品种,开展施肥量与种植密度二因素试验,设3个施肥量(600、1100、1600 kg/hm^(2))与4个种植密度(5.25万、6万、6.75万、7.5万株/hm^(2)),研究不同施肥量与种植密度对马铃薯生长、产量与水分利用效率的影响。结果表明:增加施肥量能延缓马铃薯生育期,提高种植密度能缩短生育期;随施肥量增大,马铃薯株高、茎粗、叶面积指数及叶片SPAD值均显著增大;随种植密度增加,马铃薯株高、茎粗、叶面积指数及叶片SPAD值与产量均表现为先增后减趋势,施肥量与种植密度对马铃薯株高、SPAD值与产量的影响顺序为施肥量>密度;随施肥量和群体种植密度的增加,马铃薯大薯产量、大薯数、产量、水分利用效率均呈先增后减的变化趋势,产量要素之间呈极显著正相关关系,其中,以处理F2D3的马铃薯产量与水分利用效率最大。通过二元二次回归分析表明,当施肥量达到1065.366 kg/hm^(2),种植密度达到6.746万株/hm^(2)时,马铃薯能够获得最大的产量50255.98 kg/hm^(2)。施肥量达到1044.312 kg/hm^(2),种植密度6.582万株/hm^(2)时水分利用效率达到最大110.456 kg/(hm^(2)·mm)。适宜的施肥量与种植密度能够促进马铃薯生长,有利于马铃薯产量与水分利用效率的提高,当施肥量(复合肥)介于1044.312~1065.366 kg/hm^(2)时,种植密度介于6.582万~6.746万株/hm^(2)时能实现增产和水分利用协同增加,是冷凉地区马铃薯种植推荐的高产高效模式。 展开更多
关键词 冷凉灌区 马铃薯 施肥量 种植密度 农艺性状 产量 水分利用效率
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