BACKGROUND There is no established treatment to impede the progression or restore kidney function in human chronic kidney disease(CKD).AIM To examine the efficacy of cultured human CD34+cells with enhanced proliferati...BACKGROUND There is no established treatment to impede the progression or restore kidney function in human chronic kidney disease(CKD).AIM To examine the efficacy of cultured human CD34+cells with enhanced proliferating potential in kidney injury in mice.METHODS Human umbilical cord blood(UCB)-derived CD34+cells were incubated for one week in vasculogenic conditioning medium.Vasculogenic culture significantly increased the number of CD34+cells and their ability to form endothelial progenitor cell colony-forming units.Adenineinduced tubulointerstitial injury of the kidney was induced in immunodeficient non-obese diabetic/severe combined immunodeficiency mice,and cultured human UCB-CD34+cells were administered at a dose of 1×106/mouse on days 7,14,and 21 after the start of adenine diet.RESULTS Repetitive administration of cultured UCB-CD34+cells significantly improved the time-course of kidney dysfunction in the cell therapy group compared with that in the control group.Both interstitial fibrosis and tubular damage were significantly reduced in the cell therapy group compared with those in the control group(P<0.01).Microvasculature integrity was significantly preserved(P<0.01)and macrophage infiltration into kidney tissue was dramatically decreased in the cell therapy group compared with those in the control group(P<0.001).CONCLUSION Early intervention using human cultured CD34+cells significantly improved the progression of tubulointerstitial kidney injury.Repetitive administration of cultured human UCB-CD34+cells significantly improved tubulointerstitial damage in adenine-induced kidney injury in mice via vasculoprotective and anti-inflammatory effects.展开更多
The Whole-Process People’s Democracy has become an important proposition of the socialist modernization democratic theory with Chinese characteristics,and has important value for the development of the new form of hu...The Whole-Process People’s Democracy has become an important proposition of the socialist modernization democratic theory with Chinese characteristics,and has important value for the development of the new form of human civilization.The Whole-Process People’s Democracy is not only a“democracy”system,its focus is to achieve“people’s democracy”,its unique feature is to surpass“non process”democracy with the“Whole-Process”,and create a new governance path of a new form of human civilization.Of course,the emphasis of people’s democracy on the consciousness of people’s subjective participation in the whole process also puts forward corresponding requirements for the cultural quality construction of Chinese social citizens in the new era,so it is also of great significance to pay attention to the cultivation of citizens’public spirit.展开更多
文摘BACKGROUND There is no established treatment to impede the progression or restore kidney function in human chronic kidney disease(CKD).AIM To examine the efficacy of cultured human CD34+cells with enhanced proliferating potential in kidney injury in mice.METHODS Human umbilical cord blood(UCB)-derived CD34+cells were incubated for one week in vasculogenic conditioning medium.Vasculogenic culture significantly increased the number of CD34+cells and their ability to form endothelial progenitor cell colony-forming units.Adenineinduced tubulointerstitial injury of the kidney was induced in immunodeficient non-obese diabetic/severe combined immunodeficiency mice,and cultured human UCB-CD34+cells were administered at a dose of 1×106/mouse on days 7,14,and 21 after the start of adenine diet.RESULTS Repetitive administration of cultured UCB-CD34+cells significantly improved the time-course of kidney dysfunction in the cell therapy group compared with that in the control group.Both interstitial fibrosis and tubular damage were significantly reduced in the cell therapy group compared with those in the control group(P<0.01).Microvasculature integrity was significantly preserved(P<0.01)and macrophage infiltration into kidney tissue was dramatically decreased in the cell therapy group compared with those in the control group(P<0.001).CONCLUSION Early intervention using human cultured CD34+cells significantly improved the progression of tubulointerstitial kidney injury.Repetitive administration of cultured human UCB-CD34+cells significantly improved tubulointerstitial damage in adenine-induced kidney injury in mice via vasculoprotective and anti-inflammatory effects.
基金This paper is the phased research result of the project“The Centennial Exploration Process and Innovative Development of the Communist Party of China on the Marxist Concept of Problems”(Project No.:nkxj21110104)of the Tianjin Collaborative Innovation Center of Basic Principles of Marxism in 2021The phased research results of the topic“Research on Common Prosperity and the Promotion of Democratic Process in the New Era”(Project No.:2022xs-0068)of the Independent Innovation Fund(social influence project)of Tianjin University in 2022.
文摘The Whole-Process People’s Democracy has become an important proposition of the socialist modernization democratic theory with Chinese characteristics,and has important value for the development of the new form of human civilization.The Whole-Process People’s Democracy is not only a“democracy”system,its focus is to achieve“people’s democracy”,its unique feature is to surpass“non process”democracy with the“Whole-Process”,and create a new governance path of a new form of human civilization.Of course,the emphasis of people’s democracy on the consciousness of people’s subjective participation in the whole process also puts forward corresponding requirements for the cultural quality construction of Chinese social citizens in the new era,so it is also of great significance to pay attention to the cultivation of citizens’public spirit.
