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LABELING AND QUANTITATIVE ANALYSIS OF SIX LECTIN RECEPTORS OF INTRACRANIAL GLIOMAS
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作者 杨苏敏 刘振延 +1 位作者 朱砚蕴 陈贤志 《Chinese Medical Journal》 SCIE CAS CSCD 1995年第1期37-43,共7页
The distribution of six lectin receptors, WGA, RCA-1, LCA, PSA, PNA and SBA in 111 human gliomas, 8 human normal brain tissues and 11 reactive hyperplasia of astrocytes was observed by means of avidin-biotin-peroxidas... The distribution of six lectin receptors, WGA, RCA-1, LCA, PSA, PNA and SBA in 111 human gliomas, 8 human normal brain tissues and 11 reactive hyperplasia of astrocytes was observed by means of avidin-biotin-peroxidase technique. Their grays were also quantified with the image analysis instrument. The results showed that WGA and RCA-1 might be used as markers for distinguishing well-ifferentiated astrocytomas from the reactive hyperplasia of astrocytes especially the reactivity of astrocytes had a specific feature with RCA-1. The difference in quantities of WGA, RCA-1, LCA, PSA receptors between astrocytomas, ependymomas and oligodendrogliomas, medulloblastomas might conduce to the diagnosis and classification. For astrocytomas, it was also showed that a quantity of LCA and PSA receptors was correlated with the degree of cell differentiation. Therefore, they can be used as valuable markers of the differentiation of astrocytomas. 展开更多
关键词 WGA labeling AND quantitative ANALYSIS OF SIX LECTIN RECEPTORS OF INTRACRANIAL GLIOMAS RCA LCA In
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Parallel detection and quantitative analysis of specific binding of proteins by oblique-incidence reflectivity difference technique in label-free format
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作者 DAI Jun LI Lin +4 位作者 HE LiPing RUAN KangCheng LU HuiBin JIN KuiJuan YANG GuoZhen 《Science China(Physics,Mechanics & Astronomy)》 SCIE EI CAS 2014年第11期2039-2042,共4页
In this work,we parallelly detected the specific binding between microarray targets including 12 different kinds of proteins and the probe solution containing five corresponding antibodies and quantitatively analyzed ... In this work,we parallelly detected the specific binding between microarray targets including 12 different kinds of proteins and the probe solution containing five corresponding antibodies and quantitatively analyzed the interactions between CDH13 and solution phase anti-CDH13 at six different probe concentrations by oblique-incidence reflectivity difference(OIRD)method in label-free format.The detection sensitivity reached 10 ng/mL.The experimental results indicate that the OIRD method is a promising and competing technique not only in research work but also in clinic. 展开更多
关键词 label format oblique reflectivity incidence spots quantitatively competing challenge finger
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