In this paper the results of inhibition of the Aldose reductase(AR) activity on Wistar rat lens by Quercetagetin extracted from Tagetes erects Linn and by Patuletin extracted from Tagetes patula Linn are reported.Quer...In this paper the results of inhibition of the Aldose reductase(AR) activity on Wistar rat lens by Quercetagetin extracted from Tagetes erects Linn and by Patuletin extracted from Tagetes patula Linn are reported.Quercetagetin inhibited AR of the rat lens by 93.9% at 10^(-4)M, 76.0% at 10^(-5)M and 13.3% at 10^(-6)M. Patuletin inhibited AR of the rat lens by 100% at 10^(-1)M, 80% at 10^(-5)M and 22.7% at 10^(-6)M respectively. The results show that these two flavones are lens AR Inhibitors, but further ...展开更多
分别以α-乳白蛋白、β-乳球蛋白和乳铁蛋白为乳化剂,食品级中碳链脂肪酸甘油三酯为油相,以槲皮万寿菊素为芯材,采用二级高压均质法制备槲皮万寿菊素水包油型纳米乳液。通过测定乳液粒径大小、多分散指数、Zeta电位、浊度来对比三种乳...分别以α-乳白蛋白、β-乳球蛋白和乳铁蛋白为乳化剂,食品级中碳链脂肪酸甘油三酯为油相,以槲皮万寿菊素为芯材,采用二级高压均质法制备槲皮万寿菊素水包油型纳米乳液。通过测定乳液粒径大小、多分散指数、Zeta电位、浊度来对比三种乳化剂的乳化效果,研究不同pH、离子强度、热处理对乳液理化性质的影响,利用分光光度法测定纳米乳液对槲皮万寿菊素的包埋率,并采用Lu Mi Sizer稳定性分析仪对样品稳定性进行分析检测。结果表明,乳化剂种类对乳液粒径大小、分布情况有显著影响,以α-乳白蛋白为乳化剂稳定的槲皮万寿菊素纳米乳液平均粒径最小,为285.3±3.3 nm,多分散指数为0.19±0.03,包埋率约96.8%。在不同pH、离子强度和热处理条件下,以α-乳白蛋白为乳化剂稳定的槲皮万寿菊素纳米乳液具有最优的稳定性。展开更多
Objective:To explore the role of proto-oncogene Pim-1 in the proliferation and migration of nasopharyngeal carcinoma(NPC) cells.Methods:Pim-1 expressions in NPC cell lines CNE1,CNE1-GL,CNE-2Z and C666-1 were examined ...Objective:To explore the role of proto-oncogene Pim-1 in the proliferation and migration of nasopharyngeal carcinoma(NPC) cells.Methods:Pim-1 expressions in NPC cell lines CNE1,CNE1-GL,CNE-2Z and C666-1 were examined by KT-PCR,western blotting and immunoflucesence,respectively.After CNE1,CNE1-GL and C666-1 cells were treated with different concentrations of Pim-1 special inhibitor,quercelagetin,the cell viability,colony formation rate and migration ability were analyzed.Results:Pim-1 expression was negative in well-differentiated CNE1 cells,whereas expressed weakly positive in poor-differentiated CNE-2Z cells and strongly positive in undifferentiated C666-1 cells.Interestingly,CNE1-GL cells that derived from CNE1 transfected with an Epstein Barr virus latent membrane protein-1 over-expression plasmid displayed stronger expression of Pim-1.Treatment of CNE1-GL and C666-1 cells with quercelagetin significantly decreased the cell viability,colony formation rate and migration ability but not the CNE1 cells.Conclusions:These findings suggest that Pim-1 overexpression contributes to NPC proliferation and migration,and targeting Pim-1 may be a potential treatment for anti-Pim-1-expressed NPCs.展开更多
文摘In this paper the results of inhibition of the Aldose reductase(AR) activity on Wistar rat lens by Quercetagetin extracted from Tagetes erects Linn and by Patuletin extracted from Tagetes patula Linn are reported.Quercetagetin inhibited AR of the rat lens by 93.9% at 10^(-4)M, 76.0% at 10^(-5)M and 13.3% at 10^(-6)M. Patuletin inhibited AR of the rat lens by 100% at 10^(-1)M, 80% at 10^(-5)M and 22.7% at 10^(-6)M respectively. The results show that these two flavones are lens AR Inhibitors, but further ...
文摘分别以α-乳白蛋白、β-乳球蛋白和乳铁蛋白为乳化剂,食品级中碳链脂肪酸甘油三酯为油相,以槲皮万寿菊素为芯材,采用二级高压均质法制备槲皮万寿菊素水包油型纳米乳液。通过测定乳液粒径大小、多分散指数、Zeta电位、浊度来对比三种乳化剂的乳化效果,研究不同pH、离子强度、热处理对乳液理化性质的影响,利用分光光度法测定纳米乳液对槲皮万寿菊素的包埋率,并采用Lu Mi Sizer稳定性分析仪对样品稳定性进行分析检测。结果表明,乳化剂种类对乳液粒径大小、分布情况有显著影响,以α-乳白蛋白为乳化剂稳定的槲皮万寿菊素纳米乳液平均粒径最小,为285.3±3.3 nm,多分散指数为0.19±0.03,包埋率约96.8%。在不同pH、离子强度和热处理条件下,以α-乳白蛋白为乳化剂稳定的槲皮万寿菊素纳米乳液具有最优的稳定性。
基金supported by grants from the Doctoral Program of Guangdong Medical College(B2010013)National Natural Science Foundation of China(81000073)Natural Foundation of Hainan Province of China 1811197, 310043,and 811201)
文摘Objective:To explore the role of proto-oncogene Pim-1 in the proliferation and migration of nasopharyngeal carcinoma(NPC) cells.Methods:Pim-1 expressions in NPC cell lines CNE1,CNE1-GL,CNE-2Z and C666-1 were examined by KT-PCR,western blotting and immunoflucesence,respectively.After CNE1,CNE1-GL and C666-1 cells were treated with different concentrations of Pim-1 special inhibitor,quercelagetin,the cell viability,colony formation rate and migration ability were analyzed.Results:Pim-1 expression was negative in well-differentiated CNE1 cells,whereas expressed weakly positive in poor-differentiated CNE-2Z cells and strongly positive in undifferentiated C666-1 cells.Interestingly,CNE1-GL cells that derived from CNE1 transfected with an Epstein Barr virus latent membrane protein-1 over-expression plasmid displayed stronger expression of Pim-1.Treatment of CNE1-GL and C666-1 cells with quercelagetin significantly decreased the cell viability,colony formation rate and migration ability but not the CNE1 cells.Conclusions:These findings suggest that Pim-1 overexpression contributes to NPC proliferation and migration,and targeting Pim-1 may be a potential treatment for anti-Pim-1-expressed NPCs.
