Bacterial cells rely on signaling molecules to communicate with others from the same species and induce certain genes in a process known as quorum sensing (QS). A common molecule is N-acyl homoserine lactone (AHL) whi...Bacterial cells rely on signaling molecules to communicate with others from the same species and induce certain genes in a process known as quorum sensing (QS). A common molecule is N-acyl homoserine lactone (AHL) which is responsible for the expression of virulence and other factors that allow the organisms to compete in a given environment. On the other hand, other bacteria produce certain enzymes such as AHL-lactonase that break down AHL molecules and prevent gene expression of these factors. The aim of this work was to examine the level of degradation of AHL molecules by AHL-lactonase in 62 Bacillus thuringiensis (Bt) strains isolated from Middle Tennessee, Mississippi, and Alabama. N-hexanoyl-homoserine lactone (C<sub>6</sub>-HSL) and N-3-oxo-hexanoyl homoserine lactone (3-oxo-C<sub>6</sub>-HSL), which cause Chromobacterium violaceum (CV026) to produce a purple pigment were tested at different concentrations to view the Bt lactonase activity. In addition, PCR was used to test for the presence of the lactonase gene. The results showed that among the 62 Bt strains, there were 58 that possessed the AHL-lactonase (aiiA) gene and 48 strains were able to degrade C<sub>6</sub>-HSL. At high concentrations of AHL, only 13 strains were able to completely degrade C6-HSL. In addition, degradation of 3-oxo-C<sub>6</sub>-HSL was weak compared to C<sub>6</sub>-HSL. The results also revealed that AHL lactonase was thermostable, and it was concluded that the level of degradation varies in Bt strains. Only 13 of the strains studied have potent inhibitory activity against C<sub>6</sub>-HSL, which may be good to be used in field applications to control agricultural pest.展开更多
Quorum sensing(QS)system can dynamically control the expression of proteins along with the cell growth.The promoting period of QS system has been little focused on until now.In this study,a self-induced dynamic regula...Quorum sensing(QS)system can dynamically control the expression of proteins along with the cell growth.The promoting period of QS system has been little focused on until now.In this study,a self-induced dynamic regulated expression(SIDRE)system was constructed in Escherichia coli.To enable the system suitable for the expression of enzymes,promoter engineering was used to obtain P_(luxI)mutants.To test the SIDRE system,alginate lyase AL493 and esterase Est7 were used as target protein for expression.The enzyme activity of alginate lyase and esterase reached 96.38%and 106.71%of the control strains containing the T7 promoter.In high-density fermentation,the activity of alginate lyase expressed by the SIDRE system with P_(luxI)(T-38C)as promoter was 4.34-fold of that expressed by the T7 promoter.Therefore,the P_(luxI)mutants with different promoting periods and/or different strengths show great potential in both laboratory and industrial scale for protein expression.展开更多
Objective: This study focused on the antibacterial and anti-proliferative activity of extracts from Carica papaya and Cocos nucifera roots. Methodology: The minimum inhibitory concentration and the minimum bactericida...Objective: This study focused on the antibacterial and anti-proliferative activity of extracts from Carica papaya and Cocos nucifera roots. Methodology: The minimum inhibitory concentration and the minimum bactericidal concentration of the extracts on Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, and Staphylococcus aureus were deduced by the microdilution method. The anti-biofilm activity was determined on all four strains and anti-quorum sensing activity by inhibition of violacein production in Chromobacterium violaceum. Anti-proliferative activity on prostate cultured cancer cells was evaluated by MTT assay. Sterols and triterpenes were also assayed in this study. Results: The methanolic extract of Carica papaya showed the best anti-biofilm effect with a percentage inhibition of 66.10 ± 1.79. The methanolic extract of Cocos nucifera had the strongest inhibition on the production of quorum sensing (61.42 ± 0.28). In addition, the methanolic extract of Cocos nucifera roots showed the best cytotoxic effect on prostate cancer LNCaP cell lines (IC<sub>50</sub> = 26.98 ± 2.6 μg/mL) and Carica papaya on the PC-3 lines (IC<sub>50</sub> = 127.20 ± 5.99 μg/mL). The extracts were also rich in triterpenes and sterols. Conclusion: This study provides support for the ethnomedical use of Carica papaya and Cocos nucifera roots as an antimicrobial and anticancer.展开更多
Quorum sensing(QS) is cell communication that is widely used by bacterial pathogens to coordinate the expression of several collective traits, including the production of multiple virulence factors, biofilm formation,...Quorum sensing(QS) is cell communication that is widely used by bacterial pathogens to coordinate the expression of several collective traits, including the production of multiple virulence factors, biofilm formation, and swarming motility once a population threshold is reached. Several lines of evidence indicate that QS enhances virulence of bacterial pathogens in animal models as well as in human infections; however, its relative importance for bacterial pathogenesis is still incomplete. In this review, we discuss the present evidence from in vitro and in vivo experiments in animal models, as well as from clinical studies, that link QS systems with human infections. We focus on two major QS bacterial models, the opportunistic Gram negative bacteria Pseudomonas aeruginosa and the Gram positive Staphylococcus aureus, which are also two of the main agents responsible of nosocomial and wound infections. In addition, QS communication systems in other bacterial, eukaryotic pathogens, and even immune and cancer cells are also reviewed, and finally, the new approaches proposed to combat bacterial infections by the attenuation of their QS communication systems and virulence are also discussed.展开更多
Quorum sensing(QS) is a type of microbe-microbe communication system that is widespread among the microbial world, particularly among microorganisms that are symbiotic with plants and animals. Thereby, the cell-cell s...Quorum sensing(QS) is a type of microbe-microbe communication system that is widespread among the microbial world, particularly among microorganisms that are symbiotic with plants and animals. Thereby, the cell-cell signalling is likely to occur in an anaerobic rumen environment, which is a complex microbial ecosystem. In this study, using six ruminally fistulated Liuyang black goats as experimental animals, we aimed to detect the activity of quorum sensing autoinducers(AI) both in vivo and in vitro and to clone the lux S gene that encoded autoinducer-2(AI-2) synthase of microbial samples that were collected from the rumen of goats. Neutral detergent fiber(NDF) and soluble starch were the two types of substrates that were used for in vitro fermentation. The fermented fluid samples were collected at 0, 2, 4, 6, 8, 12, 24, 36, and 48 h of incubation. The acyl-homoserine lactones(AHLs) activity was determined using gas chromatography-mass spectrometer(GC-MS) analysis. However, none of the rumen fluid extracts that were collected from the goat rumen showed the same or similar fragmentation pattern to AHLs standards. Meanwhile, the AI-2 activity, assayed using a Vibrio harveyi BB170 bioassay, was negative in all samples that were collected from the goat rumen and from in vitro fermentation fluids. Our results indicated that the activities of AHLs and AI-2 were not detected in the ruminal contents from six goats and in ruminal fluids obtained from in vitro fermentation at different sampling time-points. However, the homologues of lux S in Prevotella ruminicola were cloned from in vivo and in vitro ruminal fluids. We concluded that AHLs and AI-2 could not be detected in in vivo and in vitro ruminal fluids of goats using the current detection techniques under current dietary conditions. However, the microbes that inhabited the goat rumen had the potential ability to secrete AI-2 signaling molecules and to communicate with each other via AI-2-mediated QS because of the presence of lux S.展开更多
Objective: To investigate anti-quorum sensing(anti-QS) and anti-biofilm formation(antiBF) activities of the ethanol extracts of 388 plants. Methods: The anti-QS activity of the plant extracts was evaluated by disc-dif...Objective: To investigate anti-quorum sensing(anti-QS) and anti-biofilm formation(antiBF) activities of the ethanol extracts of 388 plants. Methods: The anti-QS activity of the plant extracts was evaluated by disc-diffusion assays using the bio-reporter strain, Chromobacterium violaceum CV017. Pseudomonas aeruginosa PAO1, Yersinia enterocolitica ATCC 9610, and Agrobacterium tumefaciens C58, which possess QS systems, were used to evaluate the antiBF activity of the plant extracts. Results: Among 388 plant extracts, the Cornus controversa(C. controversa) and Cynanchum wilfordii extracts exhibited the strongest anti-QS activity. The C. controversa extract exhibited anti-BF activity against Pseudomonas aeruginosa, Yersinia enterocolitica and Agrobacterium tumefaciens, whereas the Cynanchum wilfordii extract exhibited no anti-BF activity against Pseudomonas aeruginosa. In addition, the C. controversa extract suppressed soft rot of cabbage. Conclusions: The C. controversa extract inhibits bacterial QS and BF, and is capable of controlling soft rot. Therefore, this extract has potential for the prevention and treatment of bacterial infections and for the development of alternatives to antibiotics.展开更多
Bacteria are known to communicate with each other and regulate their activities in social networks by secreting and sensing signaling molecules called autoinducers,a process known as quorum sensing(QS).This is a growi...Bacteria are known to communicate with each other and regulate their activities in social networks by secreting and sensing signaling molecules called autoinducers,a process known as quorum sensing(QS).This is a growing area of research in which we are expanding our understanding of how bacteria collectively modify their behavior but are also involved in the crosstalk between the host and gut microbiome.This is particularly relevant in the case of pathologies associated with dysbiosis or disorders of the intestinal ecosystem.This review will examine the different QS systems and the evidence for their presence in the intestinal ecosystem.We will also provide clues on the role of QS molecules that may exert,directly or indirectly through their bacterial gossip,an influence on intestinal epithelial barrier function,intestinal inflammation,and intestinal carcinogenesis.This review aims to provide evidence on the role of QS molecules in gut physiology and the potential shared by this new player.Better understanding the impact of intestinal bacterial social networks and ultimately developing new therapeutic strategies to control intestinal disorders remains a challenge that needs to be addressed in the future.展开更多
Interference with quorum sensing(QS)represents an antivirulence strategy with a significant promise for the treatment of bacterial infections and a new approach to restoring antibiotic tolerance.Over the past two deca...Interference with quorum sensing(QS)represents an antivirulence strategy with a significant promise for the treatment of bacterial infections and a new approach to restoring antibiotic tolerance.Over the past two decades,a novel series of studies have reported that quorum quenching approaches and the discovery of quorum sensing inhibitors(QSIs)have a strong impact on the discovery of anti-infective drugs against various types of bacteria.The discovery of QSI was demonstrated to be an appropriate strategy to expand the anti-infective therapeutic approaches to complement classical antibiotics and antimicrobial agents.For the discovery of QSIs,diverse approaches exist and develop in-step with the scale of screening as well as specific QS systems.This review highlights the latest findings in strategies and methodologies for QSI screening,involving activity-based screening with bioassays,chemical methods to seek bacterial QS pathways for QSI discovery,virtual screening for QSI screening,and other potential tools for interpreting QS signaling,which are innovative routes for future efforts to discover additional QSIs to combat bacterial infections.展开更多
Surfaces submerged in seawater are colonized by various microorganisms,resulting in the formation of heterogenic marine biofilms.This work aims to evaluate the biofilm formation by Cobetia marina alex and doing a comp...Surfaces submerged in seawater are colonized by various microorganisms,resulting in the formation of heterogenic marine biofilms.This work aims to evaluate the biofilm formation by Cobetia marina alex and doing a comparative study between this promising strain with the two bacterial strains isolated previously from the Mediterranean seawater,Alexandria,Egypt.Three strains;Cobetia marina alex,Pseudoalteromonas sp.alex,and Pseudoalteromonas prydzensis alex were screened for biofilm formation using the crystal violet(CV)quantification method in a single culture.The values of biofilm formed were OD600=3.0,2.7,and 2.6,respectively leading to their selection for further evaluation.However,factors affecting biofilm formation by C.marina alex were investigated.Biofilm formation was evaluated in single and multispecies consortia.Synergistic and antagonistic interactions proved in this work lead to the belief that these bacteria have the capability to produce some interesting signal molecules N-acyl Homoserine Lactones(AHLs).展开更多
Polyphenols are the secondary metabolic products of plants and are considered as active constituents to possess therapeutic effects.To date,a vast number of scientific literature addressed the potential of polyphenols...Polyphenols are the secondary metabolic products of plants and are considered as active constituents to possess therapeutic effects.To date,a vast number of scientific literature addressed the potential of polyphenols as bio-efficient compounds owing to their structural diversity.Due to the presence of several hydroxyl groups,they are metabolized quickly due to conjugation reaction and thus,readily produce toxic metabolites as a defense material against many pathogens,reflecting their safety strategy.This review focuses on the anti-quorum sensing and biofilm inhibition activity of polyphenols,which display their potential to treat bacterial infections by combating the virulence caused by pathogenic agents.Thus,for mitigating quorum sensing-controlled pathogenesis,the use of polyphenol-based phytochemicals holds immense potential to cure infections.The application of polyphenol as sensitizing agent/adjuvant therapeutics which act in synergism with antibiotics is highly remarkable.展开更多
Quorum sensing (QS) refers to the cell communication through signaling molecules that regulate many important biological functions of bacteria by monitoring their population density. Although a wide spectrum of studie...Quorum sensing (QS) refers to the cell communication through signaling molecules that regulate many important biological functions of bacteria by monitoring their population density. Although a wide spectrum of studies on the QS system mechanisms have been carried out in experiments, mathematical modeling to explore the QS system has become a powerful approach as well. In this paper, we review the research progress of network modeling in bacterial QS to capture the system's underlying mechanisms. There are four types of QS system models for bacteria: the Gram-negative QS system model, the Gram-positive QS system model, the model for both Gram-negative and Gram-positive QS system, and the synthetic QS system model. These QS system models are mostly described by the ordinary differential equations (ODE) or partial differential equations (PDE) to study the changes of signaling molecule dynamics in time and space and the cell population density variations. Besides the deterministic simulations, the stochastic modeling approaches have also been introduced to discuss the noise effects on kinetics in QS systems. Taken together, these current modeling efforts advance our understanding of the QS system by providing systematic and quantitative dynamics description, which can hardly be obtained in experiments.展开更多
Quorum sensing refers to the phenomenon that bacteria sense signal molecules in the environment and regulate a series of genes.At present,the known quorum sensing systems in Staphylococcus aureus are the Agr system an...Quorum sensing refers to the phenomenon that bacteria sense signal molecules in the environment and regulate a series of genes.At present,the known quorum sensing systems in Staphylococcus aureus are the Agr system and the LuxS/AI-2 system.They will be activated when the bacterial concentration is equal to or greater than 107/ml,and by regulating the corresponding genes,bacteria can indirectly or directly regulate the production and degradation of biofilms,the secretion of bacterial toxins and the growth of bacteria.In this paper,we summarized the research progress of quorum sensing in S.aureus by consulting relevant literatures at home and abroad on quorum sensing in S.aureus,so as to find a new direction for the future research on S.aureus.展开更多
[Objectives]This study aimed to screen anti-quorum sensing(QS)activity from the constituent of Retiboletus kauffmanii fruit body.[Methods]Chromatographic technology was used to isolate compounds,while well diffusion a...[Objectives]This study aimed to screen anti-quorum sensing(QS)activity from the constituent of Retiboletus kauffmanii fruit body.[Methods]Chromatographic technology was used to isolate compounds,while well diffusion assay was applied to screen anti-quorum sensing activity.[Results]12 phenolic compounds were purified from extracts,three catechol-derived compounds at sub-inhibitory concentrations were found to inhibit the production of violacein in Chromobacterium violaceum for the first time.[Conclusions]Three catechol analogs isolated from R.kauffmanii fruit body extract were indicated as quorum sensing inhibitors against C.violaceum.展开更多
Objective:To investigate the effect of Corchorus olitorius aqueous fraction(COAF)on quorum sensing(QS)-regulated virulence factors and biofilm formation in Pseudomonas aeruginosa(PAO1).Methods:The preliminary screenin...Objective:To investigate the effect of Corchorus olitorius aqueous fraction(COAF)on quorum sensing(QS)-regulated virulence factors and biofilm formation in Pseudomonas aeruginosa(PAO1).Methods:The preliminary screening of the anti-QS effect of COAF was performed by evaluating the anti-pathogenic activity against Chromobacterium violaceum CV026 biosensor strain.Next,the inhibitory effects of COAF on QS-regulated pyocyanin production,proteolytic and elastolytic activities,swarming motility,and biofilm formation were evaluated in PAO1.Results:The results showed that the treatment of COAF significantly decreased the biofilm biomass,attenuated virulence factors,and inhibited swarming motility of PAO1 without affecting the growth of the bacteria in a dose-dependent manner.COAF at 2000μg/mL significantly decreased Las B elastase activity in PAO1 culture,exopolysaccharide production,swarming motility,pyocyanin level,and biomass of PAO1 by 55%(P<0.05),60%(P<0.01),61%(P<0.01),65%(P<0.01)and 73%(P<0.01),respectively.In addition,the production of violacein was decreased by 62%(P<0.01)with the treatment of a high dose of COAF.Conclusions:These findings indicate that COAF can be a potential source of anti-QS agents.展开更多
Quorum sensing is the biochemical communication system within bacterial cells which may instigate their virulence factors,and hence,is imperative for pathogenesis.Such a system optimizes metabolic and behavioral activ...Quorum sensing is the biochemical communication system within bacterial cells which may instigate their virulence factors,and hence,is imperative for pathogenesis.Such a system optimizes metabolic and behavioral activities played by the densely populated bacterial community as well as the production of the concomitant response against diffusible or secreted signals.Thus,the multi-layered signal transduction networks of quorum sensing help bacteria acclimatize to the disadvantages by increasing their confrontation ability aided by biofilm formation.Previous studies unraveled the impact of quorum sensing in the microbial interactions both within a single species and between several species.The development of the synthetic ecological models has added advantages in the studies of microbial sociability in natural habitats as well as aided in drug designing.Current review highlighted the up-to-date knowledge on the basic mechanisms of quorum sensing and its influence to accelerate the bacterial resistance against different environmental stimuli or synthetic drugs along with the factors affecting the quorum sensing system,and the harmful role of quorum sensing in food spoilage.The information gathered in this review would be beneficial to understand the metabolic cross talk as well as the virulence traits of the pathogenic microorganisms in course of their pathogenesis augmented by quorum sensing.展开更多
Quorum sensing(QS)is a regulatory system that regulates the behavior of microbial populations by sensing the concentration of signal molecules that are spontaneously produced and released by bacteria.The strategy of b...Quorum sensing(QS)is a regulatory system that regulates the behavior of microbial populations by sensing the concentration of signal molecules that are spontaneously produced and released by bacteria.The strategy of blocking the QS system and inhibiting the production of virulence factors is termed as quorum quenching(QQ).This strategy attenuates virulence without killing the pathogens,thereby weakening the selective pressure on pathogens and postponing the evolution of QQ-mediated drug resistance.In recent years,there have been significant theoretical and practical developments in the field of QS and QQ.In particular,with the development and utilization of marine resources,more and more marine microbial species have been found to be regulated by these two mechanisms,further promoting the research progress of QS and QQ.In this review,we described the diversity of QS signals and QS-related regulatory systems,and then introduced mechanisms related to QS interference,with particular emphasis on the description of natural QQ enzymes and chemicals acting as QS inhibitors.Finally,the exploitation of quorum sensing quenchers and the practical application of QQ were introduced,while some QQ strategies were proposed as promising tools in different fields such as medicine,aquaculture,agriculture and biological pollution prevention areas.展开更多
通过构建嗜水气单胞菌AH-1 Quorum Sensing(QS)2个关键调节基因ahyI,ahyR的突变菌株,来系统分析嗜水气单胞菌AH-1Ⅲ型分泌系统基因,揭示它们由QS系统调控.在ahyI突变菌中,TTSS分泌效应因子(effector)aexT量显著提高.通过构建LacZ-TTSS...通过构建嗜水气单胞菌AH-1 Quorum Sensing(QS)2个关键调节基因ahyI,ahyR的突变菌株,来系统分析嗜水气单胞菌AH-1Ⅲ型分泌系统基因,揭示它们由QS系统调控.在ahyI突变菌中,TTSS分泌效应因子(effector)aexT量显著提高.通过构建LacZ-TTSS基因启动子融合表达,进一步表明QS系统负调控编码TTSS组分的基因.展开更多
文摘Bacterial cells rely on signaling molecules to communicate with others from the same species and induce certain genes in a process known as quorum sensing (QS). A common molecule is N-acyl homoserine lactone (AHL) which is responsible for the expression of virulence and other factors that allow the organisms to compete in a given environment. On the other hand, other bacteria produce certain enzymes such as AHL-lactonase that break down AHL molecules and prevent gene expression of these factors. The aim of this work was to examine the level of degradation of AHL molecules by AHL-lactonase in 62 Bacillus thuringiensis (Bt) strains isolated from Middle Tennessee, Mississippi, and Alabama. N-hexanoyl-homoserine lactone (C<sub>6</sub>-HSL) and N-3-oxo-hexanoyl homoserine lactone (3-oxo-C<sub>6</sub>-HSL), which cause Chromobacterium violaceum (CV026) to produce a purple pigment were tested at different concentrations to view the Bt lactonase activity. In addition, PCR was used to test for the presence of the lactonase gene. The results showed that among the 62 Bt strains, there were 58 that possessed the AHL-lactonase (aiiA) gene and 48 strains were able to degrade C<sub>6</sub>-HSL. At high concentrations of AHL, only 13 strains were able to completely degrade C6-HSL. In addition, degradation of 3-oxo-C<sub>6</sub>-HSL was weak compared to C<sub>6</sub>-HSL. The results also revealed that AHL lactonase was thermostable, and it was concluded that the level of degradation varies in Bt strains. Only 13 of the strains studied have potent inhibitory activity against C<sub>6</sub>-HSL, which may be good to be used in field applications to control agricultural pest.
基金supported by the National Natural Science Foundation of China(31922072)Natural Science Foundation of Shandong Province(ZR2020JQ15)+3 种基金Project of Shandong Province Higher Educational Science and Technology Program(2019KJF012)Taishan Scholar Project of Shandong Province(tsqn201812020)China Agriculture Research System(CARS-48)Qingdao Science and Technology Demonstration and Guidance Project for Benefiting the People(20-3-4-28-nsh)。
文摘Quorum sensing(QS)system can dynamically control the expression of proteins along with the cell growth.The promoting period of QS system has been little focused on until now.In this study,a self-induced dynamic regulated expression(SIDRE)system was constructed in Escherichia coli.To enable the system suitable for the expression of enzymes,promoter engineering was used to obtain P_(luxI)mutants.To test the SIDRE system,alginate lyase AL493 and esterase Est7 were used as target protein for expression.The enzyme activity of alginate lyase and esterase reached 96.38%and 106.71%of the control strains containing the T7 promoter.In high-density fermentation,the activity of alginate lyase expressed by the SIDRE system with P_(luxI)(T-38C)as promoter was 4.34-fold of that expressed by the T7 promoter.Therefore,the P_(luxI)mutants with different promoting periods and/or different strengths show great potential in both laboratory and industrial scale for protein expression.
文摘Objective: This study focused on the antibacterial and anti-proliferative activity of extracts from Carica papaya and Cocos nucifera roots. Methodology: The minimum inhibitory concentration and the minimum bactericidal concentration of the extracts on Escherichia coli, Pseudomonas aeruginosa, Streptococcus mutans, and Staphylococcus aureus were deduced by the microdilution method. The anti-biofilm activity was determined on all four strains and anti-quorum sensing activity by inhibition of violacein production in Chromobacterium violaceum. Anti-proliferative activity on prostate cultured cancer cells was evaluated by MTT assay. Sterols and triterpenes were also assayed in this study. Results: The methanolic extract of Carica papaya showed the best anti-biofilm effect with a percentage inhibition of 66.10 ± 1.79. The methanolic extract of Cocos nucifera had the strongest inhibition on the production of quorum sensing (61.42 ± 0.28). In addition, the methanolic extract of Cocos nucifera roots showed the best cytotoxic effect on prostate cancer LNCaP cell lines (IC<sub>50</sub> = 26.98 ± 2.6 μg/mL) and Carica papaya on the PC-3 lines (IC<sub>50</sub> = 127.20 ± 5.99 μg/mL). The extracts were also rich in triterpenes and sterols. Conclusion: This study provides support for the ethnomedical use of Carica papaya and Cocos nucifera roots as an antimicrobial and anticancer.
基金Supported by JSPS grant Challenging Exploratory Research,No.25660062SEP-CONACyT grant,No.152794(García-Contreras R)+3 种基金Fideicomiso COLPOS 167304 and Programa Cátedras-CONACyT 2112(Castillo-Juárez)the Miguel Servet Program(C.H.U.A Coruña and ISCIII)(Tomás M)the CONACyT grant number 441393/269132(Mandujano-Tinoco EA)and the Biotechnology Endowed Chair at the Pennsylvania State University.
文摘Quorum sensing(QS) is cell communication that is widely used by bacterial pathogens to coordinate the expression of several collective traits, including the production of multiple virulence factors, biofilm formation, and swarming motility once a population threshold is reached. Several lines of evidence indicate that QS enhances virulence of bacterial pathogens in animal models as well as in human infections; however, its relative importance for bacterial pathogenesis is still incomplete. In this review, we discuss the present evidence from in vitro and in vivo experiments in animal models, as well as from clinical studies, that link QS systems with human infections. We focus on two major QS bacterial models, the opportunistic Gram negative bacteria Pseudomonas aeruginosa and the Gram positive Staphylococcus aureus, which are also two of the main agents responsible of nosocomial and wound infections. In addition, QS communication systems in other bacterial, eukaryotic pathogens, and even immune and cancer cells are also reviewed, and finally, the new approaches proposed to combat bacterial infections by the attenuation of their QS communication systems and virulence are also discussed.
基金financially support of the Chinese Academy of Sciences (KZCX2-YW-455)the CAS/SAFEA International Partnership Program for Creative Research Teams,China (KZCX2-YW-T07) and K C Wong Education, Hong Kong
文摘Quorum sensing(QS) is a type of microbe-microbe communication system that is widespread among the microbial world, particularly among microorganisms that are symbiotic with plants and animals. Thereby, the cell-cell signalling is likely to occur in an anaerobic rumen environment, which is a complex microbial ecosystem. In this study, using six ruminally fistulated Liuyang black goats as experimental animals, we aimed to detect the activity of quorum sensing autoinducers(AI) both in vivo and in vitro and to clone the lux S gene that encoded autoinducer-2(AI-2) synthase of microbial samples that were collected from the rumen of goats. Neutral detergent fiber(NDF) and soluble starch were the two types of substrates that were used for in vitro fermentation. The fermented fluid samples were collected at 0, 2, 4, 6, 8, 12, 24, 36, and 48 h of incubation. The acyl-homoserine lactones(AHLs) activity was determined using gas chromatography-mass spectrometer(GC-MS) analysis. However, none of the rumen fluid extracts that were collected from the goat rumen showed the same or similar fragmentation pattern to AHLs standards. Meanwhile, the AI-2 activity, assayed using a Vibrio harveyi BB170 bioassay, was negative in all samples that were collected from the goat rumen and from in vitro fermentation fluids. Our results indicated that the activities of AHLs and AI-2 were not detected in the ruminal contents from six goats and in ruminal fluids obtained from in vitro fermentation at different sampling time-points. However, the homologues of lux S in Prevotella ruminicola were cloned from in vivo and in vitro ruminal fluids. We concluded that AHLs and AI-2 could not be detected in in vivo and in vitro ruminal fluids of goats using the current detection techniques under current dietary conditions. However, the microbes that inhabited the goat rumen had the potential ability to secrete AI-2 signaling molecules and to communicate with each other via AI-2-mediated QS because of the presence of lux S.
基金supported by Basic Science Research Program through the National Research Foundation of Korea(NRF) funded by the Ministry of Education(2015R1A6A1A03031413)Rural Development Administration,Republic of Korea(PJ00743703)
文摘Objective: To investigate anti-quorum sensing(anti-QS) and anti-biofilm formation(antiBF) activities of the ethanol extracts of 388 plants. Methods: The anti-QS activity of the plant extracts was evaluated by disc-diffusion assays using the bio-reporter strain, Chromobacterium violaceum CV017. Pseudomonas aeruginosa PAO1, Yersinia enterocolitica ATCC 9610, and Agrobacterium tumefaciens C58, which possess QS systems, were used to evaluate the antiBF activity of the plant extracts. Results: Among 388 plant extracts, the Cornus controversa(C. controversa) and Cynanchum wilfordii extracts exhibited the strongest anti-QS activity. The C. controversa extract exhibited anti-BF activity against Pseudomonas aeruginosa, Yersinia enterocolitica and Agrobacterium tumefaciens, whereas the Cynanchum wilfordii extract exhibited no anti-BF activity against Pseudomonas aeruginosa. In addition, the C. controversa extract suppressed soft rot of cabbage. Conclusions: The C. controversa extract inhibits bacterial QS and BF, and is capable of controlling soft rot. Therefore, this extract has potential for the prevention and treatment of bacterial infections and for the development of alternatives to antibiotics.
基金by Fondation pour la Recherche Médicale,No.EQU202003010171Association François Aupetit,No.AHLs 2019 and No.AHLs 2021+1 种基金Fondation pour la Recherche Médical FRM,No.ECO201806006843(to Coquant G)and CORDDIM,Ile-de-France Region(to Aguanno D).
文摘Bacteria are known to communicate with each other and regulate their activities in social networks by secreting and sensing signaling molecules called autoinducers,a process known as quorum sensing(QS).This is a growing area of research in which we are expanding our understanding of how bacteria collectively modify their behavior but are also involved in the crosstalk between the host and gut microbiome.This is particularly relevant in the case of pathologies associated with dysbiosis or disorders of the intestinal ecosystem.This review will examine the different QS systems and the evidence for their presence in the intestinal ecosystem.We will also provide clues on the role of QS molecules that may exert,directly or indirectly through their bacterial gossip,an influence on intestinal epithelial barrier function,intestinal inflammation,and intestinal carcinogenesis.This review aims to provide evidence on the role of QS molecules in gut physiology and the potential shared by this new player.Better understanding the impact of intestinal bacterial social networks and ultimately developing new therapeutic strategies to control intestinal disorders remains a challenge that needs to be addressed in the future.
基金funded by the National Natural Science Foundation of China (Grant No.: 81803812)
文摘Interference with quorum sensing(QS)represents an antivirulence strategy with a significant promise for the treatment of bacterial infections and a new approach to restoring antibiotic tolerance.Over the past two decades,a novel series of studies have reported that quorum quenching approaches and the discovery of quorum sensing inhibitors(QSIs)have a strong impact on the discovery of anti-infective drugs against various types of bacteria.The discovery of QSI was demonstrated to be an appropriate strategy to expand the anti-infective therapeutic approaches to complement classical antibiotics and antimicrobial agents.For the discovery of QSIs,diverse approaches exist and develop in-step with the scale of screening as well as specific QS systems.This review highlights the latest findings in strategies and methodologies for QSI screening,involving activity-based screening with bioassays,chemical methods to seek bacterial QS pathways for QSI discovery,virtual screening for QSI screening,and other potential tools for interpreting QS signaling,which are innovative routes for future efforts to discover additional QSIs to combat bacterial infections.
文摘Surfaces submerged in seawater are colonized by various microorganisms,resulting in the formation of heterogenic marine biofilms.This work aims to evaluate the biofilm formation by Cobetia marina alex and doing a comparative study between this promising strain with the two bacterial strains isolated previously from the Mediterranean seawater,Alexandria,Egypt.Three strains;Cobetia marina alex,Pseudoalteromonas sp.alex,and Pseudoalteromonas prydzensis alex were screened for biofilm formation using the crystal violet(CV)quantification method in a single culture.The values of biofilm formed were OD600=3.0,2.7,and 2.6,respectively leading to their selection for further evaluation.However,factors affecting biofilm formation by C.marina alex were investigated.Biofilm formation was evaluated in single and multispecies consortia.Synergistic and antagonistic interactions proved in this work lead to the belief that these bacteria have the capability to produce some interesting signal molecules N-acyl Homoserine Lactones(AHLs).
文摘Polyphenols are the secondary metabolic products of plants and are considered as active constituents to possess therapeutic effects.To date,a vast number of scientific literature addressed the potential of polyphenols as bio-efficient compounds owing to their structural diversity.Due to the presence of several hydroxyl groups,they are metabolized quickly due to conjugation reaction and thus,readily produce toxic metabolites as a defense material against many pathogens,reflecting their safety strategy.This review focuses on the anti-quorum sensing and biofilm inhibition activity of polyphenols,which display their potential to treat bacterial infections by combating the virulence caused by pathogenic agents.Thus,for mitigating quorum sensing-controlled pathogenesis,the use of polyphenol-based phytochemicals holds immense potential to cure infections.The application of polyphenol as sensitizing agent/adjuvant therapeutics which act in synergism with antibiotics is highly remarkable.
基金Project supported by the National Natural Science Foundation of China(Grant Nos.11704318,11675134,and 11874310)the China Postdoctoral Science Foundation(Grant No.2016M602071).
文摘Quorum sensing (QS) refers to the cell communication through signaling molecules that regulate many important biological functions of bacteria by monitoring their population density. Although a wide spectrum of studies on the QS system mechanisms have been carried out in experiments, mathematical modeling to explore the QS system has become a powerful approach as well. In this paper, we review the research progress of network modeling in bacterial QS to capture the system's underlying mechanisms. There are four types of QS system models for bacteria: the Gram-negative QS system model, the Gram-positive QS system model, the model for both Gram-negative and Gram-positive QS system, and the synthetic QS system model. These QS system models are mostly described by the ordinary differential equations (ODE) or partial differential equations (PDE) to study the changes of signaling molecule dynamics in time and space and the cell population density variations. Besides the deterministic simulations, the stochastic modeling approaches have also been introduced to discuss the noise effects on kinetics in QS systems. Taken together, these current modeling efforts advance our understanding of the QS system by providing systematic and quantitative dynamics description, which can hardly be obtained in experiments.
基金Supported by National Natural Science Foundation(No.81460626No.81060357)Guangxi Graduate Education Innovation Program Degree and Graduate Education Reform Research Project(No.JGY2014086).
文摘Quorum sensing refers to the phenomenon that bacteria sense signal molecules in the environment and regulate a series of genes.At present,the known quorum sensing systems in Staphylococcus aureus are the Agr system and the LuxS/AI-2 system.They will be activated when the bacterial concentration is equal to or greater than 107/ml,and by regulating the corresponding genes,bacteria can indirectly or directly regulate the production and degradation of biofilms,the secretion of bacterial toxins and the growth of bacteria.In this paper,we summarized the research progress of quorum sensing in S.aureus by consulting relevant literatures at home and abroad on quorum sensing in S.aureus,so as to find a new direction for the future research on S.aureus.
基金Supported by Natural Science Foundation of Hainan Province(219MS078)Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(17CXTD-15,1630052016008)China Agriculture Research System(CARS-21).
文摘[Objectives]This study aimed to screen anti-quorum sensing(QS)activity from the constituent of Retiboletus kauffmanii fruit body.[Methods]Chromatographic technology was used to isolate compounds,while well diffusion assay was applied to screen anti-quorum sensing activity.[Results]12 phenolic compounds were purified from extracts,three catechol-derived compounds at sub-inhibitory concentrations were found to inhibit the production of violacein in Chromobacterium violaceum for the first time.[Conclusions]Three catechol analogs isolated from R.kauffmanii fruit body extract were indicated as quorum sensing inhibitors against C.violaceum.
基金supported by a grant from the“Research Center of the Female Scientific and Medical Colleges”,Deanship of Scientific Research,King Saud University
文摘Objective:To investigate the effect of Corchorus olitorius aqueous fraction(COAF)on quorum sensing(QS)-regulated virulence factors and biofilm formation in Pseudomonas aeruginosa(PAO1).Methods:The preliminary screening of the anti-QS effect of COAF was performed by evaluating the anti-pathogenic activity against Chromobacterium violaceum CV026 biosensor strain.Next,the inhibitory effects of COAF on QS-regulated pyocyanin production,proteolytic and elastolytic activities,swarming motility,and biofilm formation were evaluated in PAO1.Results:The results showed that the treatment of COAF significantly decreased the biofilm biomass,attenuated virulence factors,and inhibited swarming motility of PAO1 without affecting the growth of the bacteria in a dose-dependent manner.COAF at 2000μg/mL significantly decreased Las B elastase activity in PAO1 culture,exopolysaccharide production,swarming motility,pyocyanin level,and biomass of PAO1 by 55%(P<0.05),60%(P<0.01),61%(P<0.01),65%(P<0.01)and 73%(P<0.01),respectively.In addition,the production of violacein was decreased by 62%(P<0.01)with the treatment of a high dose of COAF.Conclusions:These findings indicate that COAF can be a potential source of anti-QS agents.
文摘Quorum sensing is the biochemical communication system within bacterial cells which may instigate their virulence factors,and hence,is imperative for pathogenesis.Such a system optimizes metabolic and behavioral activities played by the densely populated bacterial community as well as the production of the concomitant response against diffusible or secreted signals.Thus,the multi-layered signal transduction networks of quorum sensing help bacteria acclimatize to the disadvantages by increasing their confrontation ability aided by biofilm formation.Previous studies unraveled the impact of quorum sensing in the microbial interactions both within a single species and between several species.The development of the synthetic ecological models has added advantages in the studies of microbial sociability in natural habitats as well as aided in drug designing.Current review highlighted the up-to-date knowledge on the basic mechanisms of quorum sensing and its influence to accelerate the bacterial resistance against different environmental stimuli or synthetic drugs along with the factors affecting the quorum sensing system,and the harmful role of quorum sensing in food spoilage.The information gathered in this review would be beneficial to understand the metabolic cross talk as well as the virulence traits of the pathogenic microorganisms in course of their pathogenesis augmented by quorum sensing.
基金supported by the Young Elite Scientists Sponsorship Program by China Association for Science and Technology (CAST) (No. YESS20160009)the National Natural Science Foundation of China (Nos. 31870023, 31571970 and 41506160)
文摘Quorum sensing(QS)is a regulatory system that regulates the behavior of microbial populations by sensing the concentration of signal molecules that are spontaneously produced and released by bacteria.The strategy of blocking the QS system and inhibiting the production of virulence factors is termed as quorum quenching(QQ).This strategy attenuates virulence without killing the pathogens,thereby weakening the selective pressure on pathogens and postponing the evolution of QQ-mediated drug resistance.In recent years,there have been significant theoretical and practical developments in the field of QS and QQ.In particular,with the development and utilization of marine resources,more and more marine microbial species have been found to be regulated by these two mechanisms,further promoting the research progress of QS and QQ.In this review,we described the diversity of QS signals and QS-related regulatory systems,and then introduced mechanisms related to QS interference,with particular emphasis on the description of natural QQ enzymes and chemicals acting as QS inhibitors.Finally,the exploitation of quorum sensing quenchers and the practical application of QQ were introduced,while some QQ strategies were proposed as promising tools in different fields such as medicine,aquaculture,agriculture and biological pollution prevention areas.
文摘通过构建嗜水气单胞菌AH-1 Quorum Sensing(QS)2个关键调节基因ahyI,ahyR的突变菌株,来系统分析嗜水气单胞菌AH-1Ⅲ型分泌系统基因,揭示它们由QS系统调控.在ahyI突变菌中,TTSS分泌效应因子(effector)aexT量显著提高.通过构建LacZ-TTSS基因启动子融合表达,进一步表明QS系统负调控编码TTSS组分的基因.
