In this paper, one of the most classical statistical methods, Canonical Correlation Analysis (CCA) is applied to identify quantitatively the driving forces of landuse structure in Yulin Prefecture. The main analysis i...In this paper, one of the most classical statistical methods, Canonical Correlation Analysis (CCA) is applied to identify quantitatively the driving forces of landuse structure in Yulin Prefecture. The main analysis is carried out through the software SPSS with the data on the level of towns and townships in 1992. The results indicate that landuse structure is determined by comprehensive action of different factors. Landuse structure with rural characteristics is mainly determined by geographical factors such as the elevation, temperature and precipitation, while the landuse structure with urban characteristics is mainly determined by demographic and socioeconomic conditions. At the same time, tests were carried out through the canonical correlation coefficient and redundancy analysis.展开更多
The reconstruction of paleovegetation and paleoclimate requires an understanding of the relationships between surface pollen assemblages and modern vegetation and climate.Here,we analyzed the characteristics of surfac...The reconstruction of paleovegetation and paleoclimate requires an understanding of the relationships between surface pollen assemblages and modern vegetation and climate.Here,we analyzed the characteristics of surface pollen assemblages across different vegetation zones in the Tianshan Mountains.Using surface pollen analysis and vegetation sample surveys at 75 sites on the northern slopes of the Tianshan Mountains,we determined the correlation between the percentage of dominant pollen types and the corresponding vegetation cover.Redundancy analysis was used to investigate the relationships between surface pollen assemblages and environmental factors.Our results show that the Tianshan Mountains contain several distinct ecological regions,which can be divided into five main vegetation zones from low to high altitudes:mountain desert zone(Hutubi County(HTB):500-1300 m;Qitai County(QT):1000-1600 m),mountain steppe zone(HTB:1400-1600 m;QT:1650-1800 m),mountain forest zone(HTB:1650-2525 m;QT:1850-2450 m),subalpine meadow zone(HTB:2550-2600 m;QT:2500-2600 m),and alpine mat vegetation zone(HTB:2625-2700 m;QT:2625-2750 m).The surface pollen assemblages of different vegetation zones can accurately reflect the characteristics of the mountainous vegetation patterns on the northern slopes of the Tianshan Mountains when excluding the widespread occurrence of Chenopodiaceae,Artemisia,and Picea pollen.Both average annual precipitation(P_(ann))and annual average temperature(T_(ann))affect the distribution of surface pollen assemblages.Moreover,P_(ann) is the primary environmental factor affecting surface pollen assemblages in this region.A significant correlation exists between the pollen percentage and vegetation cover of Picea,Chenopodiaceae,Artemisia,and Asteraceae.Moreover,Picea,Chenopodiaceae,and Artemisia pollen are over-represented compared with their corresponding vegetation cover.The Asteraceae pollen percentage roughly reflects the distribution of a species within the local vegetation.These results have important implications for enhancing our understanding of the relationship between surface pollen assemblages and modern vegetation and climate.展开更多
Effects of suction dredging on water quality and zooplankton community structure in a shallow of eutrophic lake, were evaluated. The results showed that a decreasing trend for levels of phosphorus, organic matter, tot...Effects of suction dredging on water quality and zooplankton community structure in a shallow of eutrophic lake, were evaluated. The results showed that a decreasing trend for levels of phosphorus, organic matter, total suspended solids, Chlorophyll a and Secchi transparency in the water column was found, while levels of water depth, electrical conductivity, total dissolved solids and NO3^--N concentration increased markedly post-dredging. The effects of dredging on dissolved oxygen, pH value and temperature were almost negligible. The zooplankton community structure responded rapidly to the environmental changes caused mainly by dredging. As a result, the abundance of rotifers decreased, while the density of zooplanktonic crustaceans increased markedly. The representative taxa were Brachionus angularis, B. budapestinensis, B. diversicornis, Synchaeta spp. and Neodiaptomus schmackeri. A distinct relationship between zooplankton taxa composition and their environment, unraveled by a redundancy analysis, indicating that the measured environment contributed to the variations in the zooplankton community structure to some extent. The first four synthetic environmental variables explained 51.7% of the taxonomic structure. Therefore, with the reduction of internal nutrient load and a shift in dominance by less eutrophic species, it inferred that dredging might be one of effective measures for environmental improvements of such lakes.展开更多
To explore the spatial heterogeneity of plankton communities in a deep artificial lake （Songhua Lake, China）, samples were collected at seven sites. Samples were investigated by denaturing gradient gel electrophores...To explore the spatial heterogeneity of plankton communities in a deep artificial lake （Songhua Lake, China）, samples were collected at seven sites. Samples were investigated by denaturing gradient gel electrophoresis （DGGE） analysis of the PCR-amplified 16S and 18S rRNA genes and specific bands were sequenced. Cluster analysis of the DGGE profiles revealed that all of the samples grouped into two distinct clusters, in accordance with sampling site; while in each cluster, the divergence of sub-clusters correlated with sampling depth. Sequence analysis of selected dominant DGGE bands revealed that most sequenced phylotypes （84%） exhibited 〉97% similarity to the closest sequences in GenBank, and were affiliated with ten common freshwater plankton phyla （Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Bacillariophyta, Pyrrophyta, Cryptophyta, Ciliophora, Stramenopiles, and Rotifera）. Several of these groups are also found worldwide, indicating the cosmopolitan distribution of the phylotypes. The relationships between DGGE patterns and environmental factors were analyzed by redundancy analysis （RDA）. The results suggested that, total nitrogen, nitrate, nitrite, temperature were strongly correlated with the variation ammonia, and CODMn concentrations, and water in plankton composition.展开更多
Effects of the heavy metal copper(Cu), the metalloid arsenic(As), and the antibiotic oxytetracycline(OTC) on bacterial community structure and diversity during cow and pig manure composting were investigated. Eight tr...Effects of the heavy metal copper(Cu), the metalloid arsenic(As), and the antibiotic oxytetracycline(OTC) on bacterial community structure and diversity during cow and pig manure composting were investigated. Eight treatments were applied, four to each manure type, namely cow manure with:(1) no additives(control),(2) addition of heavy metal and metalloid,(3) addition of OTC and(4) addition of OTC with heavy metal and metalloid;and pig manure with:(5) no additives(control),(6) addition of heavy metal and metalloid,(7) addition of OTC and(8) addition of OTC with heavy metal and metalloid. After 35 days of composting, according to the alpha diversity indices, the combination treatment(OTC with heavy metal and metalloid) in pig manure was less harmful to microbial diversity than the control or heavy metal and metalloid treatments. In cow manure, the treatment with heavy metal and metalloid was the most harmful to the microbial community, followed by the combination and OTC treatments. The OTC and combination treatments had negative effects on the relative abundance of microbes in cow manure composts. The dominant phyla in both manure composts included Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. The microbial diversity relative abundance transformation was dependent on the composting time. Redundancy analysis(RDA) revealed that environmental parameters had the most influence on the bacterial communities. In conclusion, the composting process is the most sustainable technology for reducing heavy metal and metalloid impacts and antibiotic contamination in cow and pig manure. The physicochemical property variations in the manures had a significant effect on the microbial community during the composting process. This study provides an improved understanding of bacterial community composition and its changes during the composting process.展开更多
We evaluated the seasonal variation in plankton community composition in an artificial lake. We conducted microscopic analysis and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified partial 16S rRNAand 18...We evaluated the seasonal variation in plankton community composition in an artificial lake. We conducted microscopic analysis and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified partial 16S rRNAand 18S rRNAgenes to characterize the plankton community. The clustering of unweighted pair group method with arithmetic mean (UPGMA) was then used to investigate the similarity of these plankton communities. DGGE fingerprinting revealed that samples collected at the different sites within a season shared high similarity and were generally grouped together. In contrast, we did not observe any seasonal variation based on microscopic analysis. Redundancy analysis (RDA) of the plankton operational taxonomic units (OTUs) in relation to environmental factors revealed that transparency was negatively correlated with the first axis (R=-0.931), and temperature and total phosphorus (TP) were positively correlated with the first axis (R=0.736 and R=0.660, respectively). In conclusion, plankton communities in the artificial lake exhibited significant seasonal variation. Transparency, phosphorus and temperature appear to be the major factors driving the differences in plankton composition.展开更多
Reservoirs are an important water source in many densely populated areas in southwest China.Phytoplankton play an essential role in maintaining the structure and function of reservoir ecosystems.Understanding the succ...Reservoirs are an important water source in many densely populated areas in southwest China.Phytoplankton play an essential role in maintaining the structure and function of reservoir ecosystems.Understanding the succession in phytoplankton communities and the factors driving it are essential for eff ective water quality management in drinking water reservoirs.In this study,water samples were collected monthly at the surface layers from March 2016 to December 2019 in Hongfeng Reservoir,southwest China.The relationship between functional group succession was analyzed based on nonmetric multidimensional scaling analysis(NMDS),redundancy analysis(RDA),succession rate,and other analysis methods.The results showed distinct shifts in the community structure of phytoplankton functional groups within study period.The Cyclotella sp.was dominant in 2016 and 2017,and Pseudanabaena limnetica was the dominant group in 2018 and 2019.It appears that the phytoplankton composition and biomass are closely related to the water temperature and nutrient status in this reservoir.The results clearly showed that the permanganate index(COD_(Mn))was the key factor of dramatic phytoplankton functional group succession,and the change in succession rates was closely caused by total nitrogen concentration(TN).Therefore,the succession pattern and key factors of Hongfeng Reservoir revealed in this study were important guidance for the management of drinking water reservoirs in southwest China.A reasonable limit on exogenous nutrient input should be a priority,especially in high water temperature period.展开更多
Accurate evaluation of soil productivity has been a long-standing challenge. Although numerous models for productivity assessment exist, most are cumbersome to use and require substantial parameter inputs. We develope...Accurate evaluation of soil productivity has been a long-standing challenge. Although numerous models for productivity assessment exist, most are cumbersome to use and require substantial parameter inputs. We developed a new empirical soil productivity model based on field investigations of soil erosion, soil physieoehemieal properties, and crop yields in the dry-hot valleys （DHVs） in China. We found that soil pH, and organic matter and available potassium contents significantly affected crop yields under eroded conditions of the DHVs. Moreover, available potassium content was the key factor affecting soil productivity. We then modified an existing soil productivity model by adding the following parameters： contents of effective water, potassium, organic matter, and clay, soil pH, and root weighting factor. The modified soil productivity model explained 63.5% of the crop yield. We concluded that the new model was simple, realistic, and exhibited strong predictability. In addition to providing an accurate assessment of soil productivity,our model could potentially be applied as a soil module in comprehensive crop models.展开更多
This study aimed to determine the characteristics of soil microbial community composition and its relationship with soil chemical properties during natural recovery in the Loess Plateau.The soil microbial community co...This study aimed to determine the characteristics of soil microbial community composition and its relationship with soil chemical properties during natural recovery in the Loess Plateau.The soil microbial community composition was analyzed by comparing the soil microbial phospholipid fatty acids（PLFAs） of eight croplands abandoned for 1,3,5,10,13,15,20,and 30 yr in the Dunshan watershed,northern Loess Plateau,China.The results showed that soil organic carbon,total nitrogen,soil microbial biomass carbon,and soil microbial biomass nitrogen significantly increased with the abandonment duration,whereas the metabolic quotient significantly decreased.The Shannon richness and Shannon evenness of PLFAs significantly increased after 10 yr of abandonment.Gram-negative,Gram-positive,bacterial,fungal,and total PLFAs linearly increased with increased abandonment duration.Redundancy analysis showed that the abandonment duration was the most important environmental factor in determining the PLFA microbial community composition.The soil microbial PLFAs changed from anteiso-to iso-,unsaturated to saturated,and short-to long-chain during natural recovery.Therefore,in the Loess Plateau,cropland abandonment for natural recovery resulted in the increase of the soil microbial PLFA biomass and microbial PLFA species and changed the microbial from chemolithotrophic to a more heterotrophic community.展开更多
The seasonal and spatial variations of zooplankton community and theircorrelation with environmental factors were studied at nineteen sites in the coastalwater near Luan River Estuary. A total of 25 taxa of zooplankto...The seasonal and spatial variations of zooplankton community and theircorrelation with environmental factors were studied at nineteen sites in the coastalwater near Luan River Estuary. A total of 25 taxa of zooplankton were identified, amongwhich copepods being the most abundant groups (93.6% and 74.9% of totalabundance in spring and summer, respectively). Copepoda species assemblage wasthe same in spring and summer: Paracalanus parvus and Acartia bifilosa being thepredominant species, occupied more than 15% of total copepoda abundance.Zooplankton abundance was higher in spring than in summer, with an average of11 657 ind/m3 and 1549 ind/m3, respectively. One-way analysis of variance (ANOVA)showed that there was a significant difference of total zooplankton abundance betweenspring and summer (p<0.001). Redundancy analysis (RDA) revealed that salinity andnutrient were the main environmental factors influencing zooplankton group’sdistribution in spring and summer, respectively.展开更多
Acid mine drainage(AMD)has become a widespread environmental issue and its toxicity can cause permanent damage to the ecosystem.However,there are few studies focusing on the formation of AMD under moderately thermophi...Acid mine drainage(AMD)has become a widespread environmental issue and its toxicity can cause permanent damage to the ecosystem.However,there are few studies focusing on the formation of AMD under moderately thermophilic conditions,hence we employed X-ray diffraction(XRD),scanning electron microscopy(SEM),X-ray photoelectron spectroscopy(XPS)and 16S rRNA sequencing to study the dissolution of pyrite and bornite by a moderate thermophilic consortium,and explored the role of free and attached microorganisms in the formation of AMD.The consortium mainly comprised Acidithiobacillus caldus,Leptospirillum ferriphilum and Sulfobacillus thermosulfidooxidans.The results indicated that total iron in pyrite solution system reached 33.45 g/L on the 12th day,and the copper dissolution rate of bornite dissolution reached 91.8%on the 24th day.SEM results indicated that the surfaces of pyrite and bornite were significantly corroded by microorganisms.XRD and XPS results showed that ore residues contained jarosite,and the dissolving residue of bornite contained elemental sulfur.The dominant bacterial genus in pyrite dissolution was A.caldus,and L.ferriphilum in bornite dissolution.To sum up,microbes significantly accelerated the mineral dissolution process and promoted the formation of AMD.展开更多
Hydrology plays a dominant role in wetland plant distribution and microbial composition, but few studies explicitly attempted to relate the linkage between wetland vegetation and microbial community. The present study...Hydrology plays a dominant role in wetland plant distribution and microbial composition, but few studies explicitly attempted to relate the linkage between wetland vegetation and microbial community. The present study consisted of five wetland plant communities along three adjacent flood gradients zones(zone 1 dominated by Carex appendiculat, zone 2 dominated by Eleocharis ovate, and zone 3 dominated by Phragmites australis/Bidens pilosa/Calamagrostis angustifolia, which formed separate, monoculture patches). Gram negative and arbuscular mycorrhizal fungal phospholipid fatty acid(PLFA) are more abundant in the site with short flooding period(zone 3) than in the site with long flooding period(zone 1), and they are also different in the P. australis, B. spilosa and C. angustifolia of zone 3. Principle Component Analysis(PCA) showed that the flooding period could explain 92.4% of variance in microbial composition. Redundancy Analysis(RDA) showed that available nitrogen(AN), total nitrogen(TN) and soil organic matter(SOM) could explain the 79.5% of variance in microbial composition among E. ovata, P. australis, B. pilosa and C. angustifolia. Results demonstrated that flooding period was the main factor in driving the microbial composition and plant-derived resources could influence soil microbial composition in the seasonally flooded zones.展开更多
Biological soil crusts （BSCs） are bio-sedimentary associations that play crucial ecological roles in arid and semi-arid regions. In the Gurbantunggut Desert of China, more than 27% of the land surface is characteriz...Biological soil crusts （BSCs） are bio-sedimentary associations that play crucial ecological roles in arid and semi-arid regions. In the Gurbantunggut Desert of China, more than 27% of the land surface is characterized by a predominant cover of lichen-dominated BSCs that contribute to the stability of the desert. However, little is known about the major factors that limit the spatial distribution of BSCs at a macro scale. In this study, the cover of BSCs was investigated along a precipitation gradient from the margins to the center of the Gurbantunggut Desert. Environmental variables including precipitation, soil particle size, soil pH, electrical conductivity, soil organic carbon, total salt, total nitrogen, total phosphorus and total potassium were analyzed at a macro scale to determine their association with differing assemblages of BSCs （cyanobacteria crusts, lichen crusts and moss crusts） using constrained linear ordination redundancy analysis （RDA）. A model of BSCs distribution correlated with environmental variables that dominated the first two axes of the RDA was constructed to clearly demonstrate the succession stages of BSCs. The study determined that soil particle size （represented by coarse sand content） and precipitation are the most significant drivers influencing the spatial distribution of BSCs at a macro scale in the Gurbantunggut Desert. The cover of lichen and moss crusts increased with increasing precipitation, while the cover of cyanobacteria crusts decreased with increasing precipitation. The cover of lichen and moss crusts was negatively associated with coarse sand content, whereas the cover of cyanobacteria crusts was positively correlated with coarse sand content. These findings highlight the need for both the availability of soil moisture and a relatively stable of soil matrix, not only for the growth of BSCs but more importantly, for the regeneration and rehabilitation of disturbed BSC communities in arid and semi-arid lands. Thereby, this study will provide a theory basis to effectively increase soil stability in desert regions.展开更多
We characterized variations in bacterioplankton community composition(BCC) in mesocosms subject to three different treatments. Two groups contained fish(group one: Cyprinus carpio; group two: Hypophthalmichthys molitr...We characterized variations in bacterioplankton community composition(BCC) in mesocosms subject to three different treatments. Two groups contained fish(group one: Cyprinus carpio; group two: Hypophthalmichthys molitrix); and group three, the untreated mesocosm, was the control. Samples were taken seven times over a 49-d period, and BCC was analyzed by PCR-denaturing gradient gel electrophoresis(DGGE) and real-time quantitative PCR(q PCR). Results revealed that introduction of C. carpio and H. molitrix had a remarkable impact on the composition of bacterioplankton communities, and the BCC was significantly diff erent between each treatment. Sequencing of DGGE bands revealed that the bacterioplankton community in the different treatment groups was consistent at a taxonomic level, but differed in its abundance. H. molitrix promoted the richness of Alphaproteobacteria and Actinobacteria, while more bands affiliated to Cyanobacteria were detected in C. carpio mesocosms. The redundancy analysis(RDA) result demonstrated that the BCC was closely related to the bottom-up(total phosphorus, chlorophyll a, phytoplankton biomass) and top-down forces(biomass of copepods and cladocera) in C. carpio and control mesocosms, respectively. We found no evidence for top-down regulation of BCC by zooplankton in H. molitrix mesocosms, while grazing by protozoa(heterotrophic nanoflagellates, ciliates) became the major way to regulate BCC. Total bacterioplankton abundances were significantly higher in C. carpio mesocosms because of high nutrient concentration and suspended solids. Our study provided insights into the relationship between fish and bacterioplankton at species level, leading to a deep understanding of the function of the microbial loop and the aquatic ecosystem.展开更多
Based on the observed data in monitored drainage areas and GIS spatial analysis tools,watershed basic database of Shitoukoumen Reservoir Basin was built.The multivariate analysis and redundancy analysis(RDA) were used...Based on the observed data in monitored drainage areas and GIS spatial analysis tools,watershed basic database of Shitoukoumen Reservoir Basin was built.The multivariate analysis and redundancy analysis(RDA) were used to analyze the spatial and temporal variations of water quality,identify the key environmental factors and their patterns influencing the spatial variation of water quality,and determine the main types and forms of the non-point source(NPS) pollutant export controlled by the key environmental factors.The results show that different patterns of environmental factors lead to great changes in water quality at spatial and seasonal scales.All selected environmental factors explain 64.5% and 68.2% of the spatial variation of water quality over dry season and rainy season,respectively,which shows clear seasonal difference.Over dry season,residential land is the most important environmental factor,which possesses 35.4% of the spatial variation,and drainage area is the second key environmental factor,which possesses 17.0% of spatial variation in the total variance.Over rainy season,slope length and drainage area are the key environmental factors,which possess 29.3% of the spatial variation together.Residential land influences nitrogen export by changing NH4+-N and particulate organic nitrogen(PON) discharge over dry season,and drainage area controls phosphorus export by regulating dissolved phosphorus(DP) drainage over dry season and phosphorus associated particulate(PAP) loss over rainy season,respectively.Although slope length is an important environmental factor,it does not influence NPS pollutant export.It is interesting that soil organic matter,as a minor environmental factor,highly determines phosphorus and nitrogen export by enhancing the DP,PAP and PON loss.展开更多
Tillage practices and organic amendment are strategies used worldwide to preserve the properties and fertility of soils.This study aimed to elucidate effects of 3-year field treatments of tillage practice and straw ma...Tillage practices and organic amendment are strategies used worldwide to preserve the properties and fertility of soils.This study aimed to elucidate effects of 3-year field treatments of tillage practice and straw management on physical properties of Mollisols,root architecture and maize yield in northeast China.The experiment was conducted from 2015 to 2018 following a splitplot design of a randomized complete block with tillage practices[rotary tillage(R)and deep tillage(D)]as main plots and straw managements[straw returning(S),straw returning and organic fertilizer(M),straw removal(T)]as subplots.Soil samples at 0-15,15-30,30-50 cm depths and root samples at the seedling stage were collected.The results showed that DM treatment significantly improved soil moisture content at 10-50 cm soil depth and decreased soil compaction(P<0.05),which led to a better root architecture.Rotary tillage had a slower thermal conductivity but better thermal insulation performance,while deep tillage showed a higher daily temperature difference.Bulk density of topsoil was significantly lower in DS(1.16 g·cm^(-3))than in other treatments,but the soil permeability in DS(1.40 mm·min^(-1)in 0-15 soil depth and 1.45 mm·min-1in 15-30 cm soil depth)was the highest.At the maize seedling stage,DM had the highest root dry weight,root-shoot ratio and root length,while RM had the highest root volume,root furcation number and root tip number.The maize yield of three years in DM was 6.19%,5.21%and 15.72%higher than that in DS,DT and RM(P<0.01),respectively.Relative to RT and DT,a slight decrease(2.72%and 0.93%,respectively)in maize yield under RS and DS was observed,which could be alleviated by the addition of organic fertilizer.The correlation matrix indicated that kernel per ear number and 100-kernel weight were the dominant factors that affected maize yield.Redundancy analysis suggested that straw managements and tillage practices were significantly positively correlated with root-shoot ratio,root dry weight,maximum root length,the total root length and maize yield,but significantly negatively correlated with soil compaction,bulk density,soil moisture content and soil temperature.Among all the treatments,deep tillage with straw returning and the addition of organic fertilizer was recommended as a promising strategy in restoring soil productivity,promoting maize growth and increasing maize yield in Mollisols of northeast China and similar regions around the world.展开更多
Objective:Gastrointestinal heat retention syndrome(GHRS)is associated with lung-heat syndrome and is related to recurrent respiratory infection.Upper respiratory tract infection(URTI)lung heat syndrome is common in ch...Objective:Gastrointestinal heat retention syndrome(GHRS)is associated with lung-heat syndrome and is related to recurrent respiratory infection.Upper respiratory tract infection(URTI)lung heat syndrome is common in children.The study will explore the effect of GHRS on the structure and function of gut microbiota in children with URTI lung-heat syndrome.Methods:Participants were divided into both groups using the self-developed URTI scale and the“GHRS Diagnostic Scale$Pediatric Part”:GHRS-positive children(LS group)and GHRS-negative children(L group).General information,clinical symptoms,and stool were collected.We used 16S rRNA amplicon sequencing technology to determine the gene sequence of the V3eV4 region in feces and measure the gut microbiota of the both groups at the genus level.Results:A total of 23 children were included in the both groups.There were 12 cases in the LS group and 11 cases in the L group.There was no statistical difference between the both groups in age,gender,height,weight,and body mass index.The effective sequences shared by the both groups accounted for 85.66%of the total.In the gut microbiota,there was no difference in the a diversity and the b diversity between the both groups.Compared with the L group,the LS group had a significant increase in the relative abundance of the Ruminococcus gnavus group,Prevotella-9,Staphylococcus,and Actinomyces(P<.05).The functions of the both groups of microbiota primarily concentrate on metabolism,genetic information processing,and environmental information processing.The relative abundance of signaling molecules and interactions in the LS group were higher than that in the L group(P<.05).The redundancy analysis(RDA)showed that the URTI score had the greatest impact on the distribution of microbiota.Conclusion:GHRS may affect the development of URTI lung-heat syndrome by changing the relative abundances of gut microbiota.展开更多
The relationship between water quality and land use is of significance for the protection of the water environment. Here we take the Gaotan River Basin in Liangping District as the research object, and comprehensively...The relationship between water quality and land use is of significance for the protection of the water environment. Here we take the Gaotan River Basin in Liangping District as the research object, and comprehensively use the spatial analysis with geographical information system(GIS), Pearson correlation analysis, and redundancy analysis(RDA) to study the ecological response of four water quality indexes of chemical oxygen demand(COD), biochemical oxygen demand in the 5 th day(BOD5), ammonia nitrogen(NH3-N), and total phosphorus(TP) on land use patterns on a sub-basin scale. The results show that land use patterns of the Gaotan River Basin have an important impact on water quality: wooded land can improve the water environment of the river; dry land, land for urban and rural housing and public facilities, and land for mining and industrial use are the main sources of COD, BOD5, and NH3-N; garden plot, land for building communications, and water areas have a weak impact on pollutants, indicating that agricultural non-point source pollution and domestics pollution in rural areas are the major cause of the deterioration of the water quality of the Gaotan River. The research results are of guiding significance to the treatment of water environment of the Gaotan River Basin.展开更多
The insufficient supply of phosphorus is the main problem facing agriculture and forestry production in red soil region of southern China.To investigate whether applying bag-controlled slow-release fertilizer can impr...The insufficient supply of phosphorus is the main problem facing agriculture and forestry production in red soil region of southern China.To investigate whether applying bag-controlled slow-release fertilizer can improve the phosphorus availability and available phosphorus component content,with Gaofeng State-owned Eucalyptus Plantations Forest Farm in Guangxi red soil region as the research object,comparative test of different fertilizer types and fertilizer amount of bag-controlled slow-release fertilizer with conventional fertilization(G1),bag-controlled slow-release fertilizer with reducing fertilization(G2),traditional compound fertilizer(G3),basal fertilizer(G4),and no fertilization(G5)was designed.Soil phosphorus classification method was used to determine the content of phosphorus components in the soil,and redundancy analysis was used to study the effects of components on tree growth.The results showed that:compared with traditional fertilization,bag-controlled slow-release fertilizer significantly increased the contents of AP,Pi,Fe-P and other components in soil;the phosphorus activation coefficients of G2 and G1 were significantly higher than those of the other three treatments,and the order was G2>G1>G4>G5>G3;redundancy analysis showed that PAC and Fe-P had the most significant effect on plant growth among all phosphorus components.The application of bag-controlled slow-release fertilizer in red soil of eucalyptus plantation in southern China could promote the accumulation of phosphorus and increase the phosphorus activity.The mechanism of higher phosphorus use efficiency in bag-controlled slow-release fertilizer was mainly through the sustained phosphorus input and reducing phosphorus immobilization in soil by obstructing the contact of phosphorus with acid soil,to guarantee the phosphorus supply in tree growth process.展开更多
文摘In this paper, one of the most classical statistical methods, Canonical Correlation Analysis (CCA) is applied to identify quantitatively the driving forces of landuse structure in Yulin Prefecture. The main analysis is carried out through the software SPSS with the data on the level of towns and townships in 1992. The results indicate that landuse structure is determined by comprehensive action of different factors. Landuse structure with rural characteristics is mainly determined by geographical factors such as the elevation, temperature and precipitation, while the landuse structure with urban characteristics is mainly determined by demographic and socioeconomic conditions. At the same time, tests were carried out through the canonical correlation coefficient and redundancy analysis.
基金supported by the National Natural Science Foundation of China(42071102).
文摘The reconstruction of paleovegetation and paleoclimate requires an understanding of the relationships between surface pollen assemblages and modern vegetation and climate.Here,we analyzed the characteristics of surface pollen assemblages across different vegetation zones in the Tianshan Mountains.Using surface pollen analysis and vegetation sample surveys at 75 sites on the northern slopes of the Tianshan Mountains,we determined the correlation between the percentage of dominant pollen types and the corresponding vegetation cover.Redundancy analysis was used to investigate the relationships between surface pollen assemblages and environmental factors.Our results show that the Tianshan Mountains contain several distinct ecological regions,which can be divided into five main vegetation zones from low to high altitudes:mountain desert zone(Hutubi County(HTB):500-1300 m;Qitai County(QT):1000-1600 m),mountain steppe zone(HTB:1400-1600 m;QT:1650-1800 m),mountain forest zone(HTB:1650-2525 m;QT:1850-2450 m),subalpine meadow zone(HTB:2550-2600 m;QT:2500-2600 m),and alpine mat vegetation zone(HTB:2625-2700 m;QT:2625-2750 m).The surface pollen assemblages of different vegetation zones can accurately reflect the characteristics of the mountainous vegetation patterns on the northern slopes of the Tianshan Mountains when excluding the widespread occurrence of Chenopodiaceae,Artemisia,and Picea pollen.Both average annual precipitation(P_(ann))and annual average temperature(T_(ann))affect the distribution of surface pollen assemblages.Moreover,P_(ann) is the primary environmental factor affecting surface pollen assemblages in this region.A significant correlation exists between the pollen percentage and vegetation cover of Picea,Chenopodiaceae,Artemisia,and Asteraceae.Moreover,Picea,Chenopodiaceae,and Artemisia pollen are over-represented compared with their corresponding vegetation cover.The Asteraceae pollen percentage roughly reflects the distribution of a species within the local vegetation.These results have important implications for enhancing our understanding of the relationship between surface pollen assemblages and modern vegetation and climate.
基金supported by the High-Tech Research Program (863) of China (No.2009ZX07106-002-004)the National Natural Science Foundation of China (No.30870221,20877093,50808172, 50909091)
文摘Effects of suction dredging on water quality and zooplankton community structure in a shallow of eutrophic lake, were evaluated. The results showed that a decreasing trend for levels of phosphorus, organic matter, total suspended solids, Chlorophyll a and Secchi transparency in the water column was found, while levels of water depth, electrical conductivity, total dissolved solids and NO3^--N concentration increased markedly post-dredging. The effects of dredging on dissolved oxygen, pH value and temperature were almost negligible. The zooplankton community structure responded rapidly to the environmental changes caused mainly by dredging. As a result, the abundance of rotifers decreased, while the density of zooplanktonic crustaceans increased markedly. The representative taxa were Brachionus angularis, B. budapestinensis, B. diversicornis, Synchaeta spp. and Neodiaptomus schmackeri. A distinct relationship between zooplankton taxa composition and their environment, unraveled by a redundancy analysis, indicating that the measured environment contributed to the variations in the zooplankton community structure to some extent. The first four synthetic environmental variables explained 51.7% of the taxonomic structure. Therefore, with the reduction of internal nutrient load and a shift in dominance by less eutrophic species, it inferred that dredging might be one of effective measures for environmental improvements of such lakes.
基金Supported by the National Natural Science Foundation of China(No.51178090)the National Key Technology R&D Program of China(No.2012BAJ21B02-02)the National Water Pollution Control and Management Technology Major Projects(No.2009ZX07106-001)
文摘To explore the spatial heterogeneity of plankton communities in a deep artificial lake （Songhua Lake, China）, samples were collected at seven sites. Samples were investigated by denaturing gradient gel electrophoresis （DGGE） analysis of the PCR-amplified 16S and 18S rRNA genes and specific bands were sequenced. Cluster analysis of the DGGE profiles revealed that all of the samples grouped into two distinct clusters, in accordance with sampling site; while in each cluster, the divergence of sub-clusters correlated with sampling depth. Sequence analysis of selected dominant DGGE bands revealed that most sequenced phylotypes （84%） exhibited 〉97% similarity to the closest sequences in GenBank, and were affiliated with ten common freshwater plankton phyla （Proteobacteria, Actinobacteria, Bacteroidetes, Cyanobacteria, Bacillariophyta, Pyrrophyta, Cryptophyta, Ciliophora, Stramenopiles, and Rotifera）. Several of these groups are also found worldwide, indicating the cosmopolitan distribution of the phylotypes. The relationships between DGGE patterns and environmental factors were analyzed by redundancy analysis （RDA）. The results suggested that, total nitrogen, nitrate, nitrite, temperature were strongly correlated with the variation ammonia, and CODMn concentrations, and water in plankton composition.
基金the National Key Technology R&D Program of China(2018YFD0500206)the National Natural Science Foundation of China(31572209,31772395 and 31972943)the Foundation for Safety of Agricultural Products by Ministry of Agriculture and Rural Affairs,China(GJFP2019033)。
文摘Effects of the heavy metal copper(Cu), the metalloid arsenic(As), and the antibiotic oxytetracycline(OTC) on bacterial community structure and diversity during cow and pig manure composting were investigated. Eight treatments were applied, four to each manure type, namely cow manure with:(1) no additives(control),(2) addition of heavy metal and metalloid,(3) addition of OTC and(4) addition of OTC with heavy metal and metalloid;and pig manure with:(5) no additives(control),(6) addition of heavy metal and metalloid,(7) addition of OTC and(8) addition of OTC with heavy metal and metalloid. After 35 days of composting, according to the alpha diversity indices, the combination treatment(OTC with heavy metal and metalloid) in pig manure was less harmful to microbial diversity than the control or heavy metal and metalloid treatments. In cow manure, the treatment with heavy metal and metalloid was the most harmful to the microbial community, followed by the combination and OTC treatments. The OTC and combination treatments had negative effects on the relative abundance of microbes in cow manure composts. The dominant phyla in both manure composts included Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. The microbial diversity relative abundance transformation was dependent on the composting time. Redundancy analysis(RDA) revealed that environmental parameters had the most influence on the bacterial communities. In conclusion, the composting process is the most sustainable technology for reducing heavy metal and metalloid impacts and antibiotic contamination in cow and pig manure. The physicochemical property variations in the manures had a significant effect on the microbial community during the composting process. This study provides an improved understanding of bacterial community composition and its changes during the composting process.
基金Supported by the National Basic Research Program of China (973 Program) (No. 2007CB109205)the National Natural Science Foundation of China (No. 30970553)
文摘We evaluated the seasonal variation in plankton community composition in an artificial lake. We conducted microscopic analysis and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified partial 16S rRNAand 18S rRNAgenes to characterize the plankton community. The clustering of unweighted pair group method with arithmetic mean (UPGMA) was then used to investigate the similarity of these plankton communities. DGGE fingerprinting revealed that samples collected at the different sites within a season shared high similarity and were generally grouped together. In contrast, we did not observe any seasonal variation based on microscopic analysis. Redundancy analysis (RDA) of the plankton operational taxonomic units (OTUs) in relation to environmental factors revealed that transparency was negatively correlated with the first axis (R=-0.931), and temperature and total phosphorus (TP) were positively correlated with the first axis (R=0.736 and R=0.660, respectively). In conclusion, plankton communities in the artificial lake exhibited significant seasonal variation. Transparency, phosphorus and temperature appear to be the major factors driving the differences in plankton composition.
基金Supported by the National Natural Science Foundation of China(No.U1612442)the Science and Technology Foundation of Guizhou Province(Nos.[2020]6009,[2020]4Y009)Anton Brancelj was supported by Slovenian Research Agency(ARRS)(No.P1-0255)。
文摘Reservoirs are an important water source in many densely populated areas in southwest China.Phytoplankton play an essential role in maintaining the structure and function of reservoir ecosystems.Understanding the succession in phytoplankton communities and the factors driving it are essential for eff ective water quality management in drinking water reservoirs.In this study,water samples were collected monthly at the surface layers from March 2016 to December 2019 in Hongfeng Reservoir,southwest China.The relationship between functional group succession was analyzed based on nonmetric multidimensional scaling analysis(NMDS),redundancy analysis(RDA),succession rate,and other analysis methods.The results showed distinct shifts in the community structure of phytoplankton functional groups within study period.The Cyclotella sp.was dominant in 2016 and 2017,and Pseudanabaena limnetica was the dominant group in 2018 and 2019.It appears that the phytoplankton composition and biomass are closely related to the water temperature and nutrient status in this reservoir.The results clearly showed that the permanganate index(COD_(Mn))was the key factor of dramatic phytoplankton functional group succession,and the change in succession rates was closely caused by total nitrogen concentration(TN).Therefore,the succession pattern and key factors of Hongfeng Reservoir revealed in this study were important guidance for the management of drinking water reservoirs in southwest China.A reasonable limit on exogenous nutrient input should be a priority,especially in high water temperature period.
基金supported by the National Natural Science Foundation Project of China(Grant Nos.41561063,41401614 and 41401560)Non-profit Industry Research Project of Chinese Ministry of Water Resources(Grant No.201501045)Department of Water Resources of Yunnan Province:Water Science and Technology Project
文摘Accurate evaluation of soil productivity has been a long-standing challenge. Although numerous models for productivity assessment exist, most are cumbersome to use and require substantial parameter inputs. We developed a new empirical soil productivity model based on field investigations of soil erosion, soil physieoehemieal properties, and crop yields in the dry-hot valleys （DHVs） in China. We found that soil pH, and organic matter and available potassium contents significantly affected crop yields under eroded conditions of the DHVs. Moreover, available potassium content was the key factor affecting soil productivity. We then modified an existing soil productivity model by adding the following parameters： contents of effective water, potassium, organic matter, and clay, soil pH, and root weighting factor. The modified soil productivity model explained 63.5% of the crop yield. We concluded that the new model was simple, realistic, and exhibited strong predictability. In addition to providing an accurate assessment of soil productivity,our model could potentially be applied as a soil module in comprehensive crop models.
基金funded by the Strategic Technology Project of Chinese Academy of Sciences (XDA05060300)the Science and Technology Research and Development Program of Shaanxi Province, China (2011KJXX63)
文摘This study aimed to determine the characteristics of soil microbial community composition and its relationship with soil chemical properties during natural recovery in the Loess Plateau.The soil microbial community composition was analyzed by comparing the soil microbial phospholipid fatty acids（PLFAs） of eight croplands abandoned for 1,3,5,10,13,15,20,and 30 yr in the Dunshan watershed,northern Loess Plateau,China.The results showed that soil organic carbon,total nitrogen,soil microbial biomass carbon,and soil microbial biomass nitrogen significantly increased with the abandonment duration,whereas the metabolic quotient significantly decreased.The Shannon richness and Shannon evenness of PLFAs significantly increased after 10 yr of abandonment.Gram-negative,Gram-positive,bacterial,fungal,and total PLFAs linearly increased with increased abandonment duration.Redundancy analysis showed that the abandonment duration was the most important environmental factor in determining the PLFA microbial community composition.The soil microbial PLFAs changed from anteiso-to iso-,unsaturated to saturated,and short-to long-chain during natural recovery.Therefore,in the Loess Plateau,cropland abandonment for natural recovery resulted in the increase of the soil microbial PLFA biomass and microbial PLFA species and changed the microbial from chemolithotrophic to a more heterotrophic community.
文摘The seasonal and spatial variations of zooplankton community and theircorrelation with environmental factors were studied at nineteen sites in the coastalwater near Luan River Estuary. A total of 25 taxa of zooplankton were identified, amongwhich copepods being the most abundant groups (93.6% and 74.9% of totalabundance in spring and summer, respectively). Copepoda species assemblage wasthe same in spring and summer: Paracalanus parvus and Acartia bifilosa being thepredominant species, occupied more than 15% of total copepoda abundance.Zooplankton abundance was higher in spring than in summer, with an average of11 657 ind/m3 and 1549 ind/m3, respectively. One-way analysis of variance (ANOVA)showed that there was a significant difference of total zooplankton abundance betweenspring and summer (p<0.001). Redundancy analysis (RDA) revealed that salinity andnutrient were the main environmental factors influencing zooplankton group’sdistribution in spring and summer, respectively.
基金Projects(51934009,52074353)supported by the National Natural Science Foundation of ChinaProject(2019YFC1803600)supported by the National Key Research and Development Program of ChinaProject(2021JJ30855)supported by the Natural Science Foundation of Hunan Province,China。
文摘Acid mine drainage(AMD)has become a widespread environmental issue and its toxicity can cause permanent damage to the ecosystem.However,there are few studies focusing on the formation of AMD under moderately thermophilic conditions,hence we employed X-ray diffraction(XRD),scanning electron microscopy(SEM),X-ray photoelectron spectroscopy(XPS)and 16S rRNA sequencing to study the dissolution of pyrite and bornite by a moderate thermophilic consortium,and explored the role of free and attached microorganisms in the formation of AMD.The consortium mainly comprised Acidithiobacillus caldus,Leptospirillum ferriphilum and Sulfobacillus thermosulfidooxidans.The results indicated that total iron in pyrite solution system reached 33.45 g/L on the 12th day,and the copper dissolution rate of bornite dissolution reached 91.8%on the 24th day.SEM results indicated that the surfaces of pyrite and bornite were significantly corroded by microorganisms.XRD and XPS results showed that ore residues contained jarosite,and the dissolving residue of bornite contained elemental sulfur.The dominant bacterial genus in pyrite dissolution was A.caldus,and L.ferriphilum in bornite dissolution.To sum up,microbes significantly accelerated the mineral dissolution process and promoted the formation of AMD.
基金Under the auspices of National Natural Science Foundation of China(No.41361015,41271106,41271107,41501105)Open Fund of the State Environmental Protection Key Laboratory of Wetland Ecology and Vegetation Restoration,Northeast Normal University(No.130028630)
文摘Hydrology plays a dominant role in wetland plant distribution and microbial composition, but few studies explicitly attempted to relate the linkage between wetland vegetation and microbial community. The present study consisted of five wetland plant communities along three adjacent flood gradients zones(zone 1 dominated by Carex appendiculat, zone 2 dominated by Eleocharis ovate, and zone 3 dominated by Phragmites australis/Bidens pilosa/Calamagrostis angustifolia, which formed separate, monoculture patches). Gram negative and arbuscular mycorrhizal fungal phospholipid fatty acid(PLFA) are more abundant in the site with short flooding period(zone 3) than in the site with long flooding period(zone 1), and they are also different in the P. australis, B. spilosa and C. angustifolia of zone 3. Principle Component Analysis(PCA) showed that the flooding period could explain 92.4% of variance in microbial composition. Redundancy Analysis(RDA) showed that available nitrogen(AN), total nitrogen(TN) and soil organic matter(SOM) could explain the 79.5% of variance in microbial composition among E. ovata, P. australis, B. pilosa and C. angustifolia. Results demonstrated that flooding period was the main factor in driving the microbial composition and plant-derived resources could influence soil microbial composition in the seasonally flooded zones.
基金supported by the National Natural Science Foundation of China(41571256,41401296)
文摘Biological soil crusts （BSCs） are bio-sedimentary associations that play crucial ecological roles in arid and semi-arid regions. In the Gurbantunggut Desert of China, more than 27% of the land surface is characterized by a predominant cover of lichen-dominated BSCs that contribute to the stability of the desert. However, little is known about the major factors that limit the spatial distribution of BSCs at a macro scale. In this study, the cover of BSCs was investigated along a precipitation gradient from the margins to the center of the Gurbantunggut Desert. Environmental variables including precipitation, soil particle size, soil pH, electrical conductivity, soil organic carbon, total salt, total nitrogen, total phosphorus and total potassium were analyzed at a macro scale to determine their association with differing assemblages of BSCs （cyanobacteria crusts, lichen crusts and moss crusts） using constrained linear ordination redundancy analysis （RDA）. A model of BSCs distribution correlated with environmental variables that dominated the first two axes of the RDA was constructed to clearly demonstrate the succession stages of BSCs. The study determined that soil particle size （represented by coarse sand content） and precipitation are the most significant drivers influencing the spatial distribution of BSCs at a macro scale in the Gurbantunggut Desert. The cover of lichen and moss crusts increased with increasing precipitation, while the cover of cyanobacteria crusts decreased with increasing precipitation. The cover of lichen and moss crusts was negatively associated with coarse sand content, whereas the cover of cyanobacteria crusts was positively correlated with coarse sand content. These findings highlight the need for both the availability of soil moisture and a relatively stable of soil matrix, not only for the growth of BSCs but more importantly, for the regeneration and rehabilitation of disturbed BSC communities in arid and semi-arid lands. Thereby, this study will provide a theory basis to effectively increase soil stability in desert regions.
基金Supported by the National Key Technology R&D Program of China(No.2014BAC09B02)the National Water Pollution Control and Management Technology Major Projects(No.2012ZX07101-002)the High-Level Scientific Research Foundation for the Introduction of Talent(No.E07016043)
文摘We characterized variations in bacterioplankton community composition(BCC) in mesocosms subject to three different treatments. Two groups contained fish(group one: Cyprinus carpio; group two: Hypophthalmichthys molitrix); and group three, the untreated mesocosm, was the control. Samples were taken seven times over a 49-d period, and BCC was analyzed by PCR-denaturing gradient gel electrophoresis(DGGE) and real-time quantitative PCR(q PCR). Results revealed that introduction of C. carpio and H. molitrix had a remarkable impact on the composition of bacterioplankton communities, and the BCC was significantly diff erent between each treatment. Sequencing of DGGE bands revealed that the bacterioplankton community in the different treatment groups was consistent at a taxonomic level, but differed in its abundance. H. molitrix promoted the richness of Alphaproteobacteria and Actinobacteria, while more bands affiliated to Cyanobacteria were detected in C. carpio mesocosms. The redundancy analysis(RDA) result demonstrated that the BCC was closely related to the bottom-up(total phosphorus, chlorophyll a, phytoplankton biomass) and top-down forces(biomass of copepods and cladocera) in C. carpio and control mesocosms, respectively. We found no evidence for top-down regulation of BCC by zooplankton in H. molitrix mesocosms, while grazing by protozoa(heterotrophic nanoflagellates, ciliates) became the major way to regulate BCC. Total bacterioplankton abundances were significantly higher in C. carpio mesocosms because of high nutrient concentration and suspended solids. Our study provided insights into the relationship between fish and bacterioplankton at species level, leading to a deep understanding of the function of the microbial loop and the aquatic ecosystem.
基金Under the auspices of Cooperation Program of Chinese Academy of Sciences and Jilin Province (No 2006SYHZ0025)Knowledge Innovation Programs of Chinese Academy of Sciences (No KZCX2-YW-126,KZCX2-YW-Q06-2)
文摘Based on the observed data in monitored drainage areas and GIS spatial analysis tools,watershed basic database of Shitoukoumen Reservoir Basin was built.The multivariate analysis and redundancy analysis(RDA) were used to analyze the spatial and temporal variations of water quality,identify the key environmental factors and their patterns influencing the spatial variation of water quality,and determine the main types and forms of the non-point source(NPS) pollutant export controlled by the key environmental factors.The results show that different patterns of environmental factors lead to great changes in water quality at spatial and seasonal scales.All selected environmental factors explain 64.5% and 68.2% of the spatial variation of water quality over dry season and rainy season,respectively,which shows clear seasonal difference.Over dry season,residential land is the most important environmental factor,which possesses 35.4% of the spatial variation,and drainage area is the second key environmental factor,which possesses 17.0% of spatial variation in the total variance.Over rainy season,slope length and drainage area are the key environmental factors,which possess 29.3% of the spatial variation together.Residential land influences nitrogen export by changing NH4+-N and particulate organic nitrogen(PON) discharge over dry season,and drainage area controls phosphorus export by regulating dissolved phosphorus(DP) drainage over dry season and phosphorus associated particulate(PAP) loss over rainy season,respectively.Although slope length is an important environmental factor,it does not influence NPS pollutant export.It is interesting that soil organic matter,as a minor environmental factor,highly determines phosphorus and nitrogen export by enhancing the DP,PAP and PON loss.
基金the National Key Research and Developmental Program of China(2017YFD0300502-2)。
文摘Tillage practices and organic amendment are strategies used worldwide to preserve the properties and fertility of soils.This study aimed to elucidate effects of 3-year field treatments of tillage practice and straw management on physical properties of Mollisols,root architecture and maize yield in northeast China.The experiment was conducted from 2015 to 2018 following a splitplot design of a randomized complete block with tillage practices[rotary tillage(R)and deep tillage(D)]as main plots and straw managements[straw returning(S),straw returning and organic fertilizer(M),straw removal(T)]as subplots.Soil samples at 0-15,15-30,30-50 cm depths and root samples at the seedling stage were collected.The results showed that DM treatment significantly improved soil moisture content at 10-50 cm soil depth and decreased soil compaction(P<0.05),which led to a better root architecture.Rotary tillage had a slower thermal conductivity but better thermal insulation performance,while deep tillage showed a higher daily temperature difference.Bulk density of topsoil was significantly lower in DS(1.16 g·cm^(-3))than in other treatments,but the soil permeability in DS(1.40 mm·min^(-1)in 0-15 soil depth and 1.45 mm·min-1in 15-30 cm soil depth)was the highest.At the maize seedling stage,DM had the highest root dry weight,root-shoot ratio and root length,while RM had the highest root volume,root furcation number and root tip number.The maize yield of three years in DM was 6.19%,5.21%and 15.72%higher than that in DS,DT and RM(P<0.01),respectively.Relative to RT and DT,a slight decrease(2.72%and 0.93%,respectively)in maize yield under RS and DS was observed,which could be alleviated by the addition of organic fertilizer.The correlation matrix indicated that kernel per ear number and 100-kernel weight were the dominant factors that affected maize yield.Redundancy analysis suggested that straw managements and tillage practices were significantly positively correlated with root-shoot ratio,root dry weight,maximum root length,the total root length and maize yield,but significantly negatively correlated with soil compaction,bulk density,soil moisture content and soil temperature.Among all the treatments,deep tillage with straw returning and the addition of organic fertilizer was recommended as a promising strategy in restoring soil productivity,promoting maize growth and increasing maize yield in Mollisols of northeast China and similar regions around the world.
基金This study was financially supported by the National Key R&D Program of China(2018YFC1704100 and 2018YFC1704101)Beijing Municipal Natural Science Foundation(7172131).
文摘Objective:Gastrointestinal heat retention syndrome(GHRS)is associated with lung-heat syndrome and is related to recurrent respiratory infection.Upper respiratory tract infection(URTI)lung heat syndrome is common in children.The study will explore the effect of GHRS on the structure and function of gut microbiota in children with URTI lung-heat syndrome.Methods:Participants were divided into both groups using the self-developed URTI scale and the“GHRS Diagnostic Scale$Pediatric Part”:GHRS-positive children(LS group)and GHRS-negative children(L group).General information,clinical symptoms,and stool were collected.We used 16S rRNA amplicon sequencing technology to determine the gene sequence of the V3eV4 region in feces and measure the gut microbiota of the both groups at the genus level.Results:A total of 23 children were included in the both groups.There were 12 cases in the LS group and 11 cases in the L group.There was no statistical difference between the both groups in age,gender,height,weight,and body mass index.The effective sequences shared by the both groups accounted for 85.66%of the total.In the gut microbiota,there was no difference in the a diversity and the b diversity between the both groups.Compared with the L group,the LS group had a significant increase in the relative abundance of the Ruminococcus gnavus group,Prevotella-9,Staphylococcus,and Actinomyces(P<.05).The functions of the both groups of microbiota primarily concentrate on metabolism,genetic information processing,and environmental information processing.The relative abundance of signaling molecules and interactions in the LS group were higher than that in the L group(P<.05).The redundancy analysis(RDA)showed that the URTI score had the greatest impact on the distribution of microbiota.Conclusion:GHRS may affect the development of URTI lung-heat syndrome by changing the relative abundances of gut microbiota.
基金Sponsored by Research and Demonstration of Integrated Prevention and Control Measures of Rural Non-Point Source Pollution in Small and Medium Watersheds in Sichuan Province(2015SZ0212)Research on Drought Monitoring in Qinghai-Tibet Plateau Based on Remote Sensing Evapotranspiration(LPM2014003)
文摘The relationship between water quality and land use is of significance for the protection of the water environment. Here we take the Gaotan River Basin in Liangping District as the research object, and comprehensively use the spatial analysis with geographical information system(GIS), Pearson correlation analysis, and redundancy analysis(RDA) to study the ecological response of four water quality indexes of chemical oxygen demand(COD), biochemical oxygen demand in the 5 th day(BOD5), ammonia nitrogen(NH3-N), and total phosphorus(TP) on land use patterns on a sub-basin scale. The results show that land use patterns of the Gaotan River Basin have an important impact on water quality: wooded land can improve the water environment of the river; dry land, land for urban and rural housing and public facilities, and land for mining and industrial use are the main sources of COD, BOD5, and NH3-N; garden plot, land for building communications, and water areas have a weak impact on pollutants, indicating that agricultural non-point source pollution and domestics pollution in rural areas are the major cause of the deterioration of the water quality of the Gaotan River. The research results are of guiding significance to the treatment of water environment of the Gaotan River Basin.
基金Supported by Special Item of Basic Business Expenses of Guangxi Academy of Forestry Sciences(LINKE 202113)Forestry Science and Technology Promotion Demonstration Project of the Central Finance([2021]TG18)Special Fund Project for Innovation Driven Development in Guangxi(GUIKE AA17204087-11)。
文摘The insufficient supply of phosphorus is the main problem facing agriculture and forestry production in red soil region of southern China.To investigate whether applying bag-controlled slow-release fertilizer can improve the phosphorus availability and available phosphorus component content,with Gaofeng State-owned Eucalyptus Plantations Forest Farm in Guangxi red soil region as the research object,comparative test of different fertilizer types and fertilizer amount of bag-controlled slow-release fertilizer with conventional fertilization(G1),bag-controlled slow-release fertilizer with reducing fertilization(G2),traditional compound fertilizer(G3),basal fertilizer(G4),and no fertilization(G5)was designed.Soil phosphorus classification method was used to determine the content of phosphorus components in the soil,and redundancy analysis was used to study the effects of components on tree growth.The results showed that:compared with traditional fertilization,bag-controlled slow-release fertilizer significantly increased the contents of AP,Pi,Fe-P and other components in soil;the phosphorus activation coefficients of G2 and G1 were significantly higher than those of the other three treatments,and the order was G2>G1>G4>G5>G3;redundancy analysis showed that PAC and Fe-P had the most significant effect on plant growth among all phosphorus components.The application of bag-controlled slow-release fertilizer in red soil of eucalyptus plantation in southern China could promote the accumulation of phosphorus and increase the phosphorus activity.The mechanism of higher phosphorus use efficiency in bag-controlled slow-release fertilizer was mainly through the sustained phosphorus input and reducing phosphorus immobilization in soil by obstructing the contact of phosphorus with acid soil,to guarantee the phosphorus supply in tree growth process.
