Objective To construct human coagulation factorⅨmini-gene(Mini-h F9)and some nonsense mutants,detect the levels of the Mini-h F9 mRNA,and analyze the molecular mechanism of microRNA125 regulating F9gene with nonsense...Objective To construct human coagulation factorⅨmini-gene(Mini-h F9)and some nonsense mutants,detect the levels of the Mini-h F9 mRNA,and analyze the molecular mechanism of microRNA125 regulating F9gene with nonsense mutation.Methods Three nonsense mutants were obtained by using PCR mutagenesis to ana-展开更多
Vegetation change is of significant concern because it plays a crucial role in the global carbon cycle and climate.Many studies have examined recent changes in vegetation growth and the associated drivers.These driver...Vegetation change is of significant concern because it plays a crucial role in the global carbon cycle and climate.Many studies have examined recent changes in vegetation growth and the associated drivers.These drivers include both natural and human activities,but few studies have identified the regulation factors.By employing normalized difference vegetation index(NDVI)data,we analyzed the spatiotemporal pattern of vegetation change in China and then explored the driving factors.It was found that the overall greening of China has improved significantly,especially in the Loess Plateau and southwest China.The Yangtze River Delta and Bohai Rim,however,have not seen as much growth.Natural conditions are conducive to vegetation growth.Although socioeconomic development will be more beneficial for vegetation restoration,the current level and speed of development have a negative effect on vegetation.The regulation factors are considered separately since they affect both directly and indirectly.Regulation factors have accelerated vegetation growth.By understanding the factors affecting the current vegetation growth,we can provide a guide for future vegetation recovery in China and other similar countries.展开更多
Objective:To explore the possible effects and mechanism of Zhizhu Decoction(ZZD)on the pathophysiology of slow transit constipation(STC).Methods A total of 54 C57BL/6 mice was randomly divided into the following 6 gro...Objective:To explore the possible effects and mechanism of Zhizhu Decoction(ZZD)on the pathophysiology of slow transit constipation(STC).Methods A total of 54 C57BL/6 mice was randomly divided into the following 6 groups by a random number table,including control,STC model(model),positive control,and low-,medium-and high-doses ZZD treatment groups(5,10,20 g/kg,namely L,M-,and H-ZZD,respectively),9 mice in each group.Following 2-week treatment,intestinal transport rate(ITR)and fecal water content were determined,and blood and colon tissue samples were collected.Hematoxylin-eosin and periodic acid-Schiff staining were performed to evaluate the morphology of colon tissues and calculate the number of goblet cells.To determine intestinal permeability,serum levels of lipopolysaccharide(LPS),low-density lipoprotein(LDL)and mannose were measured using enzyme-linked immunosorbent assay(ELISA).Western blot analysis was carried out to detect the expression levels of intestinal tight junction proteins zona-occludens-1(ZO-1),claudin-1,occludin and recombinant mucin 2(MUC2).The mRNA expression levels of inflammatory cytokines including tumor necrosis factor(TNF)-α,interleukin(IL)-1β,IL-6,IL-4,IL-10 and IL-22 were determined using reverse transcription-quantitative reverse transcription reaction.Colon indexes of oxidative stress were measured by ELISA,and protein expression levels of colon silent information regulator 1/forkhead box O transcription factor 1(SIRT1/FoxO1)antioxidant signaling pathway were detected by Western blot.Results Compared with the model group,ITR and fecal moisture were significantly enhanced in STC mice in the M-ZZD and H-ZZD groups(P<0.01).Additionally,ZZD treatment notably increased the thickness of mucosal and muscular tissue,elevated the number of goblet cells in the colon of STC mice,reduced the secretion levels of LPS,LDL and mannose,and upregulated ZO-1,claudin-1,occludin and MUC2 expressions in the colon in a dose-dependent manner,compared with the model group(P<0.05 or P<0.01).In addition,ZZD significantly attenuated intestinal inflammation and oxidative stress and activated the SIRT1/FoxO1 signaling pathway(P<0.05 or P<0.01).Conclusion ZZD exhibited beneficial effects on the intestinal system of STC mice and alleviated intestinal inflammation and oxidative stress via activating SIRT1/FoxO1 antioxidant signaling pathway in the colon.展开更多
The genetically engineered pig is regarded as an optimal source of organ transplantation for humans and an excellent model for human disease research,given its comparable physiology to human beings.A myriad of single-...The genetically engineered pig is regarded as an optimal source of organ transplantation for humans and an excellent model for human disease research,given its comparable physiology to human beings.A myriad of single-cell RNA sequencing(sc RNA-seq)data on humans has been reported,but such data on pigs are scarce.Here,we apply sc RNA-seq technology to study the cellular heterogeneity of 3-month-old pig lungs,generating the single-cell atlas of 13,580 cells covering 16 major cell types.Based on these data,we systematically characterize the similarities and differences in the cellular cross-talk and expression patterns of respiratory virus receptors in each cell type of pig lungs compared with human lungs.Furthermore,we analyze pig lung xenotransplantation barriers and reported the cell-type expression patterns of 10 genes associated with pig-to-human immunobiological incompatibility and coagulation dysregulation.We also investigate the conserved transcription factors(TFs)and their candidate target genes and constructed five conserved TF regulatory networks in the main cell types shared by pig and human lungs.Finally,we present a comprehensive and openly accessible online platform,Scdb Lung.Our sc RNA-seq atlas of the domestic pig lung and Scdb Lung database can guide pig lung research and clinical applicability.展开更多
Ecological vulnerability refers to the degree of ecosystem disturbance,system damage,and the ability of system restoration.Although case-specific evaluations of ecological vulnerability are progressing rapidly,they ha...Ecological vulnerability refers to the degree of ecosystem disturbance,system damage,and the ability of system restoration.Although case-specific evaluations of ecological vulnerability are progressing rapidly,they have been carried out mainly in areas with intensive human activities or in harsh natural environments.Using the Web of ScienceTM core collection,this review paper summarized studies on ecological vulnerability published from 2000 to 2022 and analyzed in depth major case studies.It was found that traditional ecological vulnerability research has been addressed largely in terms of assessment models,data processing models,and analysis of influencing factors,however there was a lack of research on the process of vulnerability transformation.Because the vulnerability transformation in a hierarchical vulnerability index system is regulated by multiple factors in a heterogeneous region,it is urgent to understand how the ecological vulnerability in a region evolves from one level to another over time.Therefore this paper put forward the new perspective of research,i.e.,applying quantitative analysis to identification of regulating factors and exploring the mechanisms of ecological vulnerability transformation.This new perspective could assist in monitoring the complex spatiotemporal changes in ecological vulnerability and taking necessary measures to prevent from decline of ecological stability.展开更多
文摘Objective To construct human coagulation factorⅨmini-gene(Mini-h F9)and some nonsense mutants,detect the levels of the Mini-h F9 mRNA,and analyze the molecular mechanism of microRNA125 regulating F9gene with nonsense mutation.Methods Three nonsense mutants were obtained by using PCR mutagenesis to ana-
基金National Key R&D Program of China,No.2018YFD1100101National Natural Science Foundation of China,No.71573250。
文摘Vegetation change is of significant concern because it plays a crucial role in the global carbon cycle and climate.Many studies have examined recent changes in vegetation growth and the associated drivers.These drivers include both natural and human activities,but few studies have identified the regulation factors.By employing normalized difference vegetation index(NDVI)data,we analyzed the spatiotemporal pattern of vegetation change in China and then explored the driving factors.It was found that the overall greening of China has improved significantly,especially in the Loess Plateau and southwest China.The Yangtze River Delta and Bohai Rim,however,have not seen as much growth.Natural conditions are conducive to vegetation growth.Although socioeconomic development will be more beneficial for vegetation restoration,the current level and speed of development have a negative effect on vegetation.The regulation factors are considered separately since they affect both directly and indirectly.Regulation factors have accelerated vegetation growth.By understanding the factors affecting the current vegetation growth,we can provide a guide for future vegetation recovery in China and other similar countries.
基金Supported by the General Project of National Natural Science Foundation of China(No.82074429)。
文摘Objective:To explore the possible effects and mechanism of Zhizhu Decoction(ZZD)on the pathophysiology of slow transit constipation(STC).Methods A total of 54 C57BL/6 mice was randomly divided into the following 6 groups by a random number table,including control,STC model(model),positive control,and low-,medium-and high-doses ZZD treatment groups(5,10,20 g/kg,namely L,M-,and H-ZZD,respectively),9 mice in each group.Following 2-week treatment,intestinal transport rate(ITR)and fecal water content were determined,and blood and colon tissue samples were collected.Hematoxylin-eosin and periodic acid-Schiff staining were performed to evaluate the morphology of colon tissues and calculate the number of goblet cells.To determine intestinal permeability,serum levels of lipopolysaccharide(LPS),low-density lipoprotein(LDL)and mannose were measured using enzyme-linked immunosorbent assay(ELISA).Western blot analysis was carried out to detect the expression levels of intestinal tight junction proteins zona-occludens-1(ZO-1),claudin-1,occludin and recombinant mucin 2(MUC2).The mRNA expression levels of inflammatory cytokines including tumor necrosis factor(TNF)-α,interleukin(IL)-1β,IL-6,IL-4,IL-10 and IL-22 were determined using reverse transcription-quantitative reverse transcription reaction.Colon indexes of oxidative stress were measured by ELISA,and protein expression levels of colon silent information regulator 1/forkhead box O transcription factor 1(SIRT1/FoxO1)antioxidant signaling pathway were detected by Western blot.Results Compared with the model group,ITR and fecal moisture were significantly enhanced in STC mice in the M-ZZD and H-ZZD groups(P<0.01).Additionally,ZZD treatment notably increased the thickness of mucosal and muscular tissue,elevated the number of goblet cells in the colon of STC mice,reduced the secretion levels of LPS,LDL and mannose,and upregulated ZO-1,claudin-1,occludin and MUC2 expressions in the colon in a dose-dependent manner,compared with the model group(P<0.05 or P<0.01).In addition,ZZD significantly attenuated intestinal inflammation and oxidative stress and activated the SIRT1/FoxO1 signaling pathway(P<0.05 or P<0.01).Conclusion ZZD exhibited beneficial effects on the intestinal system of STC mice and alleviated intestinal inflammation and oxidative stress via activating SIRT1/FoxO1 antioxidant signaling pathway in the colon.
基金supported by China National Gene Bank(CNGB)financially supported by the National Natural Science Foundation of China(31670742)the Natural Science Foundation of Guangdong Province,China(2021A1515011109)。
文摘The genetically engineered pig is regarded as an optimal source of organ transplantation for humans and an excellent model for human disease research,given its comparable physiology to human beings.A myriad of single-cell RNA sequencing(sc RNA-seq)data on humans has been reported,but such data on pigs are scarce.Here,we apply sc RNA-seq technology to study the cellular heterogeneity of 3-month-old pig lungs,generating the single-cell atlas of 13,580 cells covering 16 major cell types.Based on these data,we systematically characterize the similarities and differences in the cellular cross-talk and expression patterns of respiratory virus receptors in each cell type of pig lungs compared with human lungs.Furthermore,we analyze pig lung xenotransplantation barriers and reported the cell-type expression patterns of 10 genes associated with pig-to-human immunobiological incompatibility and coagulation dysregulation.We also investigate the conserved transcription factors(TFs)and their candidate target genes and constructed five conserved TF regulatory networks in the main cell types shared by pig and human lungs.Finally,we present a comprehensive and openly accessible online platform,Scdb Lung.Our sc RNA-seq atlas of the domestic pig lung and Scdb Lung database can guide pig lung research and clinical applicability.
基金This research was funded by Shaanxi Science and Technology Department(2022JQ-282)National Natural Science Foundation of China(42007415)。
文摘Ecological vulnerability refers to the degree of ecosystem disturbance,system damage,and the ability of system restoration.Although case-specific evaluations of ecological vulnerability are progressing rapidly,they have been carried out mainly in areas with intensive human activities or in harsh natural environments.Using the Web of ScienceTM core collection,this review paper summarized studies on ecological vulnerability published from 2000 to 2022 and analyzed in depth major case studies.It was found that traditional ecological vulnerability research has been addressed largely in terms of assessment models,data processing models,and analysis of influencing factors,however there was a lack of research on the process of vulnerability transformation.Because the vulnerability transformation in a hierarchical vulnerability index system is regulated by multiple factors in a heterogeneous region,it is urgent to understand how the ecological vulnerability in a region evolves from one level to another over time.Therefore this paper put forward the new perspective of research,i.e.,applying quantitative analysis to identification of regulating factors and exploring the mechanisms of ecological vulnerability transformation.This new perspective could assist in monitoring the complex spatiotemporal changes in ecological vulnerability and taking necessary measures to prevent from decline of ecological stability.
