This paper based on the essay [1]. studies in case that replicated observations are available in some experimental points, the parameters estimation of one dimensional linear errors-in-variables (EV) models. Asymptoti...This paper based on the essay [1]. studies in case that replicated observations are available in some experimental points, the parameters estimation of one dimensional linear errors-in-variables (EV) models. Asymptotic normality is established.展开更多
Marbling is regarded as an economically important trait of beef cattle inJapan, and measured as a beef marbling score (BMS). Our previous study reported an association between a single nucleotide polymorphism (SNP), r...Marbling is regarded as an economically important trait of beef cattle inJapan, and measured as a beef marbling score (BMS). Our previous study reported an association between a single nucleotide polymorphism (SNP), rs4164 8172, in the pancreatic lipase (PNLIP) gene and the BMS level, using the Japanese Black beef cattle population of Oita prefecture. Further, we showed that the T allele at the rs41648172 SNP is associated with a high level of the BMS. Thus, we suggested that the rs41648172 SNP seems to be a candidate marker for marker-assisted selection. Our present study was designed to investigate whether this association could be replicated in other independent Japanese Black cattle population and analyze the effect of the SNP genotypes on the carcass traits other than the BMS. We detected the marginally significant effect of the genotypes of the rs41648172 SNP on the BMS level by using the Japanese Black beef cattle population of Niigata prefecture (P = 0.0919), and obtained the result of the T allele associated with an increase in the BMS level, consistent with our previous data. In addition, we showed no significant association of the SNP with the subcutaneous fat thickness, carcass weight, rib eye area, rib thickness and yield estimate in the Japanese Black beef cattle population ofNiigataprefecture. Thus, we concluded that the rs41648172 SNP was useful for effective marker-assisted selection to increase the BMS level in Japanese Black beef cattle, based on the replicated association of the rs41648172 SNP with the BMS level in the other independent Japanese Black beef cattle population and no effect of the SNP genotypes on the carcass traits other than BMS.展开更多
Marbling, defined by the amount and the distribution of intramuscular fat and measured as beef marbling score (BMS), is an economically important trait of beef cattle in Japan. We recently reported that a single nucle...Marbling, defined by the amount and the distribution of intramuscular fat and measured as beef marbling score (BMS), is an economically important trait of beef cattle in Japan. We recently reported that a single nucleotide polymorphism (SNP), namely, c.-312A > G, in the endothelial differentiation sphingolipid G-proteincoupled receptor, 1 (EDG1) gene was associated with the BMS level in the Japanese Black beef cattle population of Oita prefecture, with the G allele being associated with a high level of the BMS. Thus, the c.-312A > G SNP seems to be a candidate marker for marker-assisted selection. In this study, we investigated whether this association could be replicated in the Japanese Black beef cattle population of Niigata prefecture and analyzed the effect of the SNP genotypes on the carcass traits other than the BMS. No significant differences in the BMS level were detected among the genotypes of the c.-312A > G SNP in the Niigata Japanese Black beef cattle population. The SNP genotype had no significant effects on the carcass weight, rib eye area and rib thickness of the cattle population. These findings suggested that the association of the c.-312A > G SNP with the BMS level in the Japanese Black beef cattle population was not replicated in the Niigata population, and revealed no effects of the SNP genotype on the beef productivity in the Niigata population. Thus, we concluded that the c.-312A > G SNP is not useful for effective marker-assisted selection to increase meat quality and, additionally, meat productivity in Japanese Black beef cattle of Niigata prefecture.展开更多
The aim of this work is to construct the parameter estimators in the partial linear errors-in-variables (EV) models and explore their asymptotic properties. Unlike other related References, the assumption of known err...The aim of this work is to construct the parameter estimators in the partial linear errors-in-variables (EV) models and explore their asymptotic properties. Unlike other related References, the assumption of known error covariance matrix is removed when the sample can be repeatedly drawn at each designed point from the model. The estimators of interested regression parameters, and the model error variance, as well as the nonparametric function, are constructed. Under some regular conditions, all of the estimators prove strongly consistent. Meanwhile, the asymptotic normality for the estimator of regression parameter is also presented. A simulation study is reported to illustrate our asymptotic results.展开更多
The interplay between DNA replication stress and immune microenvironment alterations is known to play a crucial role in colorectal tumorigenesis,but a comprehensive understanding of their association with and relevant...The interplay between DNA replication stress and immune microenvironment alterations is known to play a crucial role in colorectal tumorigenesis,but a comprehensive understanding of their association with and relevant biomarkers involved in colorectal tumorigenesis is lacking.To address this gap,we conducted a study aiming to investigate this association and identify relevant biomarkers.We analyzed transcriptomic and proteomic profiles of 904 colorectal tumor tissues and 342 normal tissues to examine pathway enrichment,biological activity,and the immune microenvironment.Additionally,we evaluated genetic effects of single variants and genes on colorectal cancer susceptibility using data from genome-wide association studies(GWASs)involving both East Asian(7062 cases and 195745 controls)and European(24476 cases and 23073 controls)populations.We employed mediation analysis to infer the causal pathway,and applied multiplex immunofluorescence to visualize colocalized biomarkers in colorectal tumors and immune cells.Our findings revealed that both DNA replication activity and the flap structure-specific endonuclease 1(FEN1)gene were significantly enriched in colorectal tumor tissues,compared with normal tissues.Moreover,a genetic variant rs4246215 G>T in FEN1 was associated with a decreased risk of colorectal cancer(odds ratio=0.94,95%confidence interval:0.90–0.97,P_(meta)=4.70×10^(-9)).Importantly,we identified basophils and eosinophils that both exhibited a significantly decreased infiltration in colorectal tumors,and were regulated by rs4246215 through causal pathways involving both FEN1 and DNA replication.In conclusion,this trans-omics incorporating GWAS data provides insights into a plausible pathway connecting DNA replication and immunity,expanding biological knowledge of colorectal tumorigenesis and therapeutic targets.展开更多
State machine replication has been widely used in modern cluster-based database systems.Most commonly deployed configurations adopt the Raft-like consensus protocol,which has a single strong leader which replicates th...State machine replication has been widely used in modern cluster-based database systems.Most commonly deployed configurations adopt the Raft-like consensus protocol,which has a single strong leader which replicates the log to other followers.Since the followers can handle read requests and many real workloads are usually read-intensive,the recovery speed of a crashed follower may significantly impact on the throughput.Different from traditional database recovery,the recovering follower needs to repair its local log first.Original Raft protocol takes many network round trips to do log comparison between leader and the crashed follower.To reduce network round trips,an optimization method is to truncate the follower’s uncertain log entries behind the latest local commit point,and then to directly fetch all committed log entries from the leader in one round trip.However,if the commit point is not persisted,the recovering follower has to get the whole log from the leader.In this paper,we propose an accurate and efficient log repair(AELR)algorithm for follower recovery.AELR is more robust and resilient to follower failure,and it only needs one network round trip to fetch the least number of log entries for follower recovery.This approach is implemented in the open source database system OceanBase.We experimentally show that the system adopting AELR has a good performance in terms of recovery time.展开更多
Conspecific negative density dependence(CNDD)is a potentially important mechanism in maintaining species diversity.While previous evidence showed habitat heterogeneity and species’dispersal modes affect the strength ...Conspecific negative density dependence(CNDD)is a potentially important mechanism in maintaining species diversity.While previous evidence showed habitat heterogeneity and species’dispersal modes affect the strength of CNDD at early life stages of trees(e.g.,seedlings),it remains unclear how they affect the strength of CNDD at later life stages.We examined the degree of spatial aggregation between saplings and trees for species dispersed by wind and gravity in four topographic habitats within a 25-ha temperate forest dynamic plot in the Qinling Mountains of central China.We used the replicated spatial point pattern(RSPP)analysis and bivariate paircorrelation function(PCF)to detect the spatial distribution of saplings around trees at two scales,15 and 50 m,respectively.Although the signal was not apparent across the whole study region(or 25-ha),it is distinct on isolated areas with specific characteristics,suggesting that these characteristics could be important factors in CNDD.Further,we found that the gravity-dispersed tree species experienced CNDD across habitats,while for wind-dispersed species CNDD was found in gully,terrace and low-ridge habitats.Our study suggests that neglecting the habitat heterogeneity and dispersal mode can distort the signal of CNDD and community assembly in temperate forests.展开更多
Large-scale genetic population used for genetic breeding researches covers a large area in the field experiment,and the effect of local control would be gradually weakened.The block in replication(BIR)design is suitab...Large-scale genetic population used for genetic breeding researches covers a large area in the field experiment,and the effect of local control would be gradually weakened.The block in replication(BIR)design is suitable for large population,which is applied to the field experiment of genetic population.The statistical methods of analysis of variance(ANOVA)and heritability estimation in single and multiple environments were derived and implemented using the statistical analysis system(SAS)program for the analysis of BIR.As a work example,a comparison of statistical analysis between BIR design and the completely random block(CRB)design were conducted for the protein content from a panel containing 455 soybean germplasms.The results indicated the different estimates of average heritability in multiple environments.The research results provided technical support for the application of BIR design in genetics and breeding studies.展开更多
As the amount of data continues to grow rapidly,the variety of data produced by applications is becoming more affluent than ever.Cloud computing is the best technology evolving today to provide multi-services for the ...As the amount of data continues to grow rapidly,the variety of data produced by applications is becoming more affluent than ever.Cloud computing is the best technology evolving today to provide multi-services for the mass and variety of data.The cloud computing features are capable of processing,managing,and storing all sorts of data.Although data is stored in many high-end nodes,either in the same data centers or across many data centers in cloud,performance issues are still inevitable.The cloud replication strategy is one of best solutions to address risk of performance degradation in the cloud environment.The real challenge here is developing the right data replication strategy with minimal data movement that guarantees efficient network usage,low fault tolerance,and minimal replication frequency.The key problem discussed in this research is inefficient network usage discovered during selecting a suitable data center to store replica copies induced by inadequate data center selection criteria.Hence,to mitigate the issue,we proposed Replication Strategy with a comprehensive Data Center Selection Method(RS-DCSM),which can determine the appropriate data center to place replicas by considering three key factors:Popularity,space availability,and centrality.The proposed RS-DCSM was simulated using CloudSim and the results proved that data movement between data centers is significantly reduced by 14%reduction in overall replication frequency and 20%decrement in network usage,which outperformed the current replication strategy,known as Dynamic Popularity aware Replication Strategy(DPRS)algorithm.展开更多
The fungal pathogen Setosphaeria turcica causes northern corn leaf blight(NCLB),which leads to considerable crop losses.Setosphaeria turcica elaborates a specialized infection structures called appressorium for maize ...The fungal pathogen Setosphaeria turcica causes northern corn leaf blight(NCLB),which leads to considerable crop losses.Setosphaeria turcica elaborates a specialized infection structures called appressorium for maize infection.Previously,we demonstrated that the S.turcica triggers an S-phase checkpoint and ATR(Ataxia Telangiectasia and Rad3 related)-dependent self-protective response to DNA genotoxic insults during maize infection.However,how the regulatory mechanism works was still largely unknown.Here,we report a genome wide transcriptional profile analysis during appressorium formation in the present of DNA replication stress.We performed RNA-Seq analysis to identify S.tuicica genes responsive to DNA replication stress.In the current work,we found that appressorium-mediated maize infection by S.turcica is significantly blocked by S-phase checkpoint.A large serial of secondary metabolite and melanin biosynthesis genes were blocked in appressorium formation of S.turcica during the replication stress.The secondary metabolite biosynthesis genes including alcohol dehydrogenase GroES-like domain,multicopper oxidase,ABCtransporter families,cytochrome P450 and FAD-containing monooxygenase were related to plant pathogen infection.In addition,we demonstrated that autophagy in S.turcica is up-regulated by ATR as a defense response to stress.We identified StATG3,StATG4,StATG5,StATG7 and StATG16 genes for autophagy were induced by ATR-mediated S-phase checkpoint.We therefore propose that in response to genotoxic stress,S.turcica utilizes ATR-dependent pathway to turn off transcription of genes governing appressorium-mediated infection,and meanwhile inducing transcription of autophagy genes likely as a mechanism of self-protection,aside from the more conservative responses in eukaryotes.展开更多
Background:New Omicron subvariants are emerging rapidly from BA.1 to BA.4 and BA.5.Their pathogenicity has changed from that of wild-type(WH-09)and Omicron variants have over time become globally dominant.The spike pr...Background:New Omicron subvariants are emerging rapidly from BA.1 to BA.4 and BA.5.Their pathogenicity has changed from that of wild-type(WH-09)and Omicron variants have over time become globally dominant.The spike proteins of BA.4 and BA.5 that serve as the target for vaccine-induced neutralizing antibodies have also changed compared to the previous subvariants,which is likely to cause immune es-cape and the reduction of the protective effect of the vaccine.Our study addresses the above issues and provides a basis for formulating relevant prevention and control strategies.Methods:We collected cellular supernatant and cell lysates and measured the viral titers,viral RNA loads,and E subgenomic RNA(E sgRNA)loads in different Omicron subvariants grown in Vero E6 cells,using WH-09 and Delta variants as a reference.Additionally,we evaluated the in vitro neutralizing activity of different Omicron sub-variants and compared it to the WH-09 and Delta variants using macaque sera with different types of immunity.Results:As the SARS-CoV-2 evolved into Omicron BA.1,the replication ability in vitro began to decrease.Then with the emergence of new subvariants,the replication ability gradually recovered and became stable in the BA.4 and BA.5 subvariants.In WH-09-inactivated vaccine sera,geometric mean titers of neutralization antibodies against different Omicron subvariants declined by 3.7~15.4-fold compared to those against WH-09.In Delta-inactivated vaccine sera,geometric mean titers of neutrali-zation antibodies against Omicron subvariants declined by 3.1~7.4-fold compared to those against Delta.Conclusion:According to the findings of this research,the replication efficiency of all Omicron subvariants declined compared with WH-09 and Delta variants,and was lower in BA.1 than in other Omicron subvariants.After two doses of inactivated(WH-09 or Delta)vaccine,cross-neutralizing activities against various Omicron subvariants were seen despite a decline in neutralizing titers.展开更多
The impact of a Distributed Denial of Service(DDoS)attack on Soft-ware Defined Networks(SDN)is briefly analyzed.Many approaches to detecting DDoS attacks exist,varying on the feature being considered and the method us...The impact of a Distributed Denial of Service(DDoS)attack on Soft-ware Defined Networks(SDN)is briefly analyzed.Many approaches to detecting DDoS attacks exist,varying on the feature being considered and the method used.Still,the methods have a deficiency in the performance of detecting DDoS attacks and mitigating them.To improve the performance of SDN,an efficient Real-time Multi-Constrained Adaptive Replication and Traffic Approximation Model(RMCARTAM)is sketched in this article.The RMCARTAM considers different parameters or constraints in running different controllers responsible for handling incoming packets.The model is designed with multiple controllers to handle net-work traffic but can turn the controllers according to requirements.The multi-con-straint adaptive replication model monitors different features of network traffic like rate of packet reception,class-based packet reception and target-specific reception.According to these features,the method estimates the Replication Turn-ing Weight(RTW)based on which triggering controllers are performed.Similarly,the method applies Traffic Approximation(TA)in the detection of DDoS attacks.The detection of a DDoS attack is performed by approximating the incoming traf-fic to any service and using various features like hop count,payload,service fre-quency,and malformed frequency to compute various support measures on bandwidth access,data support,frequency support,malformed support,route sup-port,and so on.Using all these support measures,the method computes the value of legitimate weight to conclude the behavior of any source in identifying the mal-icious node.Identified node details are used in the mitigation of DDoS attacks.The method stimulates the network performance by reducing the power factor by switching the controller according to different factors,which also reduces the cost.In the same way,the proposed model improves the accuracy of detecting DDoS attacks by estimating the features of incoming traffic in different corners.展开更多
Each rock joint is unique by nature which means that utilization of replicas in direct shear tests is required in experimental parameter studies.However,a method to acquire knowledge about the ability of the replicas ...Each rock joint is unique by nature which means that utilization of replicas in direct shear tests is required in experimental parameter studies.However,a method to acquire knowledge about the ability of the replicas to imitate the shear mechanical behavior of the rock joint and their dispersion in direct shear testing is lacking.In this study,a novel method is presented for geometric quality assurance of replicas.The aim is to facilitate generation of high-quality direct shear testing data as a prerequisite for reliable subsequent analyses of the results.In Part 1 of this study,two quality assurance parameters,smf and V_(Hp100),are derived and their usefulness for evaluation of geometric deviations,i.e.geometric reproducibility,is shown.In Part 2,the parameters are validated by showing a correlation between the parameters and the shear mechanical behavior,which qualifies the parameters for usage in the quality assurance method.Unique results from direct shear tests presenting comparisons between replicas and the rock joint show that replicas fulfilling proposed threshold values of σ_(mf)<0.06 mm and|V_(Hp100)|<0.2 mm have a narrow dispersion and imitate the shear mechanical behavior of the rock joint in all aspects apart from having a slightly lower peak shear strength.The wear in these replicas,which have similar morphology as the rock joint,is in the same areas as in the rock joint.The wear is slightly larger in the rock joint and therefore the discrepancy in peak shear strength derives from differences in material properties,possibly from differences in toughness.It is shown by application of the suggested method that the quality assured replicas manufactured following the process employed in this study phenomenologically capture the shear strength characteristics,which makes them useful in parameter studies.展开更多
The reliability and availability of cloud systems have become major concerns of service providers,brokers,and end-users.Therefore,studying fault-tolerance mechanisms in cloud computing attracts intense attention in in...The reliability and availability of cloud systems have become major concerns of service providers,brokers,and end-users.Therefore,studying fault-tolerance mechanisms in cloud computing attracts intense attention in industry and academia.The task-scheduling mechanisms can improve the fault-tolerance level of cloud systems.A task-scheduling mechanism distributes tasks to a group of instances to be executed.Much work has been undertaken in this direction to improve the overall outcome of cloud computing,such as improving service qual-ity and reducing power consumption.However,little work on task scheduling has studied the problem of lost tasks from the broker’s perspective.Task loss can hap-pen due to virtual machine failures,server crashes,connection interruption,etc.The broker-based concept means that the backup task can be allocated by the bro-ker on the same cloud service provider(CSP)or a different CSP to reduce costs,for example.This paper proposes a novel fault-tolerant mechanism that employs the primary backup(PB)model of task scheduling to address this issue.The pro-posed mechanism minimizes the impact of failure events by reducing the number of lost tasks.The mechanism is further improved to shorten the makespan time of submitted tasks in cloud systems.The experiments demonstrated that the pro-posed mechanism decreased the number of lost tasks by about 13%–15%com-pared with other mechanisms in the literature.展开更多
Many cutting-edge methods are now possible in real-time commercial settings and are growing in popularity on cloud platforms.By incorporating new,cutting-edge technologies to a larger extent without using more infrast...Many cutting-edge methods are now possible in real-time commercial settings and are growing in popularity on cloud platforms.By incorporating new,cutting-edge technologies to a larger extent without using more infrastructures,the information technology platform is anticipating a completely new level of devel-opment.The following concepts are proposed in this research paper:1)A reliable authentication method Data replication that is optimised;graph-based data encryp-tion and packing colouring in Redundant Array of Independent Disks(RAID)sto-rage.At the data centre,data is encrypted using crypto keys called Key Streams.These keys are produced using the packing colouring method in the web graph’s jump graph.In order to achieve space efficiency,the replication is carried out on optimised many servers employing packing colours.It would be thought that more connections would provide better authentication.This study provides an innovative architecture with robust security,enhanced authentication,and low cost.展开更多
Most social networks allow connections amongst many people based on shared interests.Social networks have to offer shared data like videos,photos with minimum latency to the group,which could be challenging as the sto...Most social networks allow connections amongst many people based on shared interests.Social networks have to offer shared data like videos,photos with minimum latency to the group,which could be challenging as the storage cost has to be minimized and hence entire data replication is not a solution.The replication of data across a network of read-intensive can potentially lead to increased savings in cost and energy and reduce the end-user’s response time.Though simple and adaptive replication strategies exist,the solution is non-deter-ministic;the replicas of the data need to be optimized to the data usability,perfor-mance,and stability of the application systems.To resolve the non-deterministic issue of replication,metaheuristics are applied.In this work,Harmony Search and Tabu Search algorithms are used optimizing the replication process.A novel Har-mony-Tabu search is proposed for effective placement and replication of data.Experiments on large datasets show the effectiveness of the proposed technique.It is seen that the bandwidth saving for proposed harmony-Tabu replication per-forms better in the range of 3.57%to 18.18%for varying number of cloud data-centers when compared to simple replication,Tabu replication and Harmony replication algorithm.展开更多
Tomato yellow leaf curl virus(TYLCV)is the dominating pathogen of tomato yellow leaf curl disease that caused severe loss to tomato production in China.In this study,we found that a TYLCV-resistant tomato line drastic...Tomato yellow leaf curl virus(TYLCV)is the dominating pathogen of tomato yellow leaf curl disease that caused severe loss to tomato production in China.In this study,we found that a TYLCV-resistant tomato line drastically reduced the accumulation of viral complementary-sense strand mRNAs but just moderately inhibited that of viral DNA and virion-sense strand mRNAs.However,two other resistant lines did not have such virus inhibition pattern.Analysis of differential expressed genes showed that the potential host defense-relevant processes varied in different resistant tomatoes,as compared to the susceptible line,suggesting a diversity of tomato TYLCV-resistance mechanisms.展开更多
Memetics confirms the importance of recitation and imitation in college English teaching.Starting with the explanation of the replication cycle of language memes,this paper discusses how to use the memetic perspective...Memetics confirms the importance of recitation and imitation in college English teaching.Starting with the explanation of the replication cycle of language memes,this paper discusses how to use the memetic perspective in college English listening and speaking teaching,effectively transform the input language information into language output,and improve the classroom effect of college English listening and speaking teaching.展开更多
Background:This study aimed to select compounds with unique inhibitory effects on muscle-invasive bladder cancer(MIBC)from coumarone derivatives with similar parent nuclear structures and to reveal their tumor-suppres...Background:This study aimed to select compounds with unique inhibitory effects on muscle-invasive bladder cancer(MIBC)from coumarone derivatives with similar parent nuclear structures and to reveal their tumor-suppressive effects using various approaches.Methods:Bladder cancer cell lines SW780 and T24,as well as human normal bladder epithelial cell line SV-HUC-1 were selected as the study model,and these urinary system cells were co-incubated with various concentrations of(S,E)-4-(4-methylbenzylidene)-3-phenylchroman-3-ol,(S,E)-4-(4-isocyanobenzylidene)-3-phenylchroman-3-ol,(S,E)-4-(4-fluorobenzylidene)-3-phenylchroman-3-ol(FPO),and(S,E)-3-phenyl-4-(4-(trifluoromethoxy)benzylidene)chroman-3-ol.Cell activity was detected using cell counting kit-8.FPO showed the strongest inhibitory effect on MIBC cells;therefore,it was selected for further experiments.We monitored the FPO-induced T24 cell morphological changes with an inverted microscope.The FPO-inhibited migration of T24 cells was examined using a cell scratch assay.We detected the clonogenic ability of T24 cells through a clone formation test and evaluated their proliferative ability using a 5-ethynyl-2’-deoxyuridine fluorescence staining kit.The inhibitory effect of FPO against the cell cycle was monitored using flow cytometry,and its suppressive effect on the DNA replication ability of T24 cells was detected using double fluorescence staining(Ki67 and phalloidin).Results:Among the four candidate coumarone derivatives,FPO showed the most significant inhibitory effect on MIBC cells and was less toxic to normal urothelial cells.FPO inhibited T24 cell growth in time and dose-dependent manners(the half-inhibitory concentration is 8μM).FPO significantly repressed the proliferation,migration,and clonogenic ability of bladder cancer T24 cells.Cell mobility was significantly inhibited by FPO:30μM FPO almost completely repressed migration occurred at after 24 h treatment.Moreover,FPO significantly suppressed the clonogenicity of bladder cancer cells in a dose-dependent manner.Mechanistically,FPO targeted the cell cycle,arresting the S and G2 phases on bladder cancer T24 cells.Conclusion:We discovered a novel anticancer chemical,FPO,and proposed a potential mechanism,through which it suppresses MIBC T24 cells by repressing the cell cycle in the S and G2 phases.This study contributes to the development of novel anticancer drugs for MIBC.展开更多
基金the National Natural Science Foundation of China (Grant No. 19631040)
文摘This paper based on the essay [1]. studies in case that replicated observations are available in some experimental points, the parameters estimation of one dimensional linear errors-in-variables (EV) models. Asymptotic normality is established.
文摘Marbling is regarded as an economically important trait of beef cattle inJapan, and measured as a beef marbling score (BMS). Our previous study reported an association between a single nucleotide polymorphism (SNP), rs4164 8172, in the pancreatic lipase (PNLIP) gene and the BMS level, using the Japanese Black beef cattle population of Oita prefecture. Further, we showed that the T allele at the rs41648172 SNP is associated with a high level of the BMS. Thus, we suggested that the rs41648172 SNP seems to be a candidate marker for marker-assisted selection. Our present study was designed to investigate whether this association could be replicated in other independent Japanese Black cattle population and analyze the effect of the SNP genotypes on the carcass traits other than the BMS. We detected the marginally significant effect of the genotypes of the rs41648172 SNP on the BMS level by using the Japanese Black beef cattle population of Niigata prefecture (P = 0.0919), and obtained the result of the T allele associated with an increase in the BMS level, consistent with our previous data. In addition, we showed no significant association of the SNP with the subcutaneous fat thickness, carcass weight, rib eye area, rib thickness and yield estimate in the Japanese Black beef cattle population ofNiigataprefecture. Thus, we concluded that the rs41648172 SNP was useful for effective marker-assisted selection to increase the BMS level in Japanese Black beef cattle, based on the replicated association of the rs41648172 SNP with the BMS level in the other independent Japanese Black beef cattle population and no effect of the SNP genotypes on the carcass traits other than BMS.
文摘Marbling, defined by the amount and the distribution of intramuscular fat and measured as beef marbling score (BMS), is an economically important trait of beef cattle in Japan. We recently reported that a single nucleotide polymorphism (SNP), namely, c.-312A > G, in the endothelial differentiation sphingolipid G-proteincoupled receptor, 1 (EDG1) gene was associated with the BMS level in the Japanese Black beef cattle population of Oita prefecture, with the G allele being associated with a high level of the BMS. Thus, the c.-312A > G SNP seems to be a candidate marker for marker-assisted selection. In this study, we investigated whether this association could be replicated in the Japanese Black beef cattle population of Niigata prefecture and analyzed the effect of the SNP genotypes on the carcass traits other than the BMS. No significant differences in the BMS level were detected among the genotypes of the c.-312A > G SNP in the Niigata Japanese Black beef cattle population. The SNP genotype had no significant effects on the carcass weight, rib eye area and rib thickness of the cattle population. These findings suggested that the association of the c.-312A > G SNP with the BMS level in the Japanese Black beef cattle population was not replicated in the Niigata population, and revealed no effects of the SNP genotype on the beef productivity in the Niigata population. Thus, we concluded that the c.-312A > G SNP is not useful for effective marker-assisted selection to increase meat quality and, additionally, meat productivity in Japanese Black beef cattle of Niigata prefecture.
基金This work was partially supported by the National Natural Science Foundation of China(Grant No.10071009)Research Foundation of Doctorial Programme(Grant No.20020027010)the Excellent Young Teacher Programme of the Ministry of Educatioin of China
文摘The aim of this work is to construct the parameter estimators in the partial linear errors-in-variables (EV) models and explore their asymptotic properties. Unlike other related References, the assumption of known error covariance matrix is removed when the sample can be repeatedly drawn at each designed point from the model. The estimators of interested regression parameters, and the model error variance, as well as the nonparametric function, are constructed. Under some regular conditions, all of the estimators prove strongly consistent. Meanwhile, the asymptotic normality for the estimator of regression parameter is also presented. A simulation study is reported to illustrate our asymptotic results.
基金supported by the National Natural Science Foundation of China(Grant No.82173601)Yili&Jiangsu Joint Institute of Health(Grant No.yl2021ms02).
文摘The interplay between DNA replication stress and immune microenvironment alterations is known to play a crucial role in colorectal tumorigenesis,but a comprehensive understanding of their association with and relevant biomarkers involved in colorectal tumorigenesis is lacking.To address this gap,we conducted a study aiming to investigate this association and identify relevant biomarkers.We analyzed transcriptomic and proteomic profiles of 904 colorectal tumor tissues and 342 normal tissues to examine pathway enrichment,biological activity,and the immune microenvironment.Additionally,we evaluated genetic effects of single variants and genes on colorectal cancer susceptibility using data from genome-wide association studies(GWASs)involving both East Asian(7062 cases and 195745 controls)and European(24476 cases and 23073 controls)populations.We employed mediation analysis to infer the causal pathway,and applied multiplex immunofluorescence to visualize colocalized biomarkers in colorectal tumors and immune cells.Our findings revealed that both DNA replication activity and the flap structure-specific endonuclease 1(FEN1)gene were significantly enriched in colorectal tumor tissues,compared with normal tissues.Moreover,a genetic variant rs4246215 G>T in FEN1 was associated with a decreased risk of colorectal cancer(odds ratio=0.94,95%confidence interval:0.90–0.97,P_(meta)=4.70×10^(-9)).Importantly,we identified basophils and eosinophils that both exhibited a significantly decreased infiltration in colorectal tumors,and were regulated by rs4246215 through causal pathways involving both FEN1 and DNA replication.In conclusion,this trans-omics incorporating GWAS data provides insights into a plausible pathway connecting DNA replication and immunity,expanding biological knowledge of colorectal tumorigenesis and therapeutic targets.
基金This research was supported in part by National Key R&D Program of China(2018YFB1003303)the National Natural Science Foundation of China(Grant Nos.61432006,61732014 and 61972149).
文摘State machine replication has been widely used in modern cluster-based database systems.Most commonly deployed configurations adopt the Raft-like consensus protocol,which has a single strong leader which replicates the log to other followers.Since the followers can handle read requests and many real workloads are usually read-intensive,the recovery speed of a crashed follower may significantly impact on the throughput.Different from traditional database recovery,the recovering follower needs to repair its local log first.Original Raft protocol takes many network round trips to do log comparison between leader and the crashed follower.To reduce network round trips,an optimization method is to truncate the follower’s uncertain log entries behind the latest local commit point,and then to directly fetch all committed log entries from the leader in one round trip.However,if the commit point is not persisted,the recovering follower has to get the whole log from the leader.In this paper,we propose an accurate and efficient log repair(AELR)algorithm for follower recovery.AELR is more robust and resilient to follower failure,and it only needs one network round trip to fetch the least number of log entries for follower recovery.This approach is implemented in the open source database system OceanBase.We experimentally show that the system adopting AELR has a good performance in terms of recovery time.
基金Shihong Jia was financially supported by the National Natural Science Foundation of China(Grant No.32001120)the Fundamental Research Funds for the Central Universities(Grant No.31020200QD026)+1 种基金Qiulong Yin was supported by the National Natural Science Foundation of China(Grant No.32001171)Ying Luo was supported by the Innovation Capability Support Program of Shaanxi(Grant No.2022KRM090).
文摘Conspecific negative density dependence(CNDD)is a potentially important mechanism in maintaining species diversity.While previous evidence showed habitat heterogeneity and species’dispersal modes affect the strength of CNDD at early life stages of trees(e.g.,seedlings),it remains unclear how they affect the strength of CNDD at later life stages.We examined the degree of spatial aggregation between saplings and trees for species dispersed by wind and gravity in four topographic habitats within a 25-ha temperate forest dynamic plot in the Qinling Mountains of central China.We used the replicated spatial point pattern(RSPP)analysis and bivariate paircorrelation function(PCF)to detect the spatial distribution of saplings around trees at two scales,15 and 50 m,respectively.Although the signal was not apparent across the whole study region(or 25-ha),it is distinct on isolated areas with specific characteristics,suggesting that these characteristics could be important factors in CNDD.Further,we found that the gravity-dispersed tree species experienced CNDD across habitats,while for wind-dispersed species CNDD was found in gully,terrace and low-ridge habitats.Our study suggests that neglecting the habitat heterogeneity and dispersal mode can distort the signal of CNDD and community assembly in temperate forests.
基金Supported by Key Research and Development Project of Heilongjiang Province(GA21B009-6)Heilongjiang Province Natural Science Foundation(C2015009)。
文摘Large-scale genetic population used for genetic breeding researches covers a large area in the field experiment,and the effect of local control would be gradually weakened.The block in replication(BIR)design is suitable for large population,which is applied to the field experiment of genetic population.The statistical methods of analysis of variance(ANOVA)and heritability estimation in single and multiple environments were derived and implemented using the statistical analysis system(SAS)program for the analysis of BIR.As a work example,a comparison of statistical analysis between BIR design and the completely random block(CRB)design were conducted for the protein content from a panel containing 455 soybean germplasms.The results indicated the different estimates of average heritability in multiple environments.The research results provided technical support for the application of BIR design in genetics and breeding studies.
基金supported by Universiti Putra Malaysia and the Ministry of Education(MOE).
文摘As the amount of data continues to grow rapidly,the variety of data produced by applications is becoming more affluent than ever.Cloud computing is the best technology evolving today to provide multi-services for the mass and variety of data.The cloud computing features are capable of processing,managing,and storing all sorts of data.Although data is stored in many high-end nodes,either in the same data centers or across many data centers in cloud,performance issues are still inevitable.The cloud replication strategy is one of best solutions to address risk of performance degradation in the cloud environment.The real challenge here is developing the right data replication strategy with minimal data movement that guarantees efficient network usage,low fault tolerance,and minimal replication frequency.The key problem discussed in this research is inefficient network usage discovered during selecting a suitable data center to store replica copies induced by inadequate data center selection criteria.Hence,to mitigate the issue,we proposed Replication Strategy with a comprehensive Data Center Selection Method(RS-DCSM),which can determine the appropriate data center to place replicas by considering three key factors:Popularity,space availability,and centrality.The proposed RS-DCSM was simulated using CloudSim and the results proved that data movement between data centers is significantly reduced by 14%reduction in overall replication frequency and 20%decrement in network usage,which outperformed the current replication strategy,known as Dynamic Popularity aware Replication Strategy(DPRS)algorithm.
基金supported by the grants from the Youth Top Talent Project from Hebei Provincial Department of Education,China(BJ2020003)the China Agriculture Research System of MOF and MARA(CARS-02-25)+3 种基金the State Key Laboratory of North China Crop Improvement and RegulationOpen Project of Key Laboratory of Microbial Diversity Research and Application of Hebei Province(MDRA202101)the Hebei Provincial Department of Bureau of Science and Technology(360-0803-JSN-3YGS)the Natural Science Foundation of Hebei Province(C202204138)。
文摘The fungal pathogen Setosphaeria turcica causes northern corn leaf blight(NCLB),which leads to considerable crop losses.Setosphaeria turcica elaborates a specialized infection structures called appressorium for maize infection.Previously,we demonstrated that the S.turcica triggers an S-phase checkpoint and ATR(Ataxia Telangiectasia and Rad3 related)-dependent self-protective response to DNA genotoxic insults during maize infection.However,how the regulatory mechanism works was still largely unknown.Here,we report a genome wide transcriptional profile analysis during appressorium formation in the present of DNA replication stress.We performed RNA-Seq analysis to identify S.tuicica genes responsive to DNA replication stress.In the current work,we found that appressorium-mediated maize infection by S.turcica is significantly blocked by S-phase checkpoint.A large serial of secondary metabolite and melanin biosynthesis genes were blocked in appressorium formation of S.turcica during the replication stress.The secondary metabolite biosynthesis genes including alcohol dehydrogenase GroES-like domain,multicopper oxidase,ABCtransporter families,cytochrome P450 and FAD-containing monooxygenase were related to plant pathogen infection.In addition,we demonstrated that autophagy in S.turcica is up-regulated by ATR as a defense response to stress.We identified StATG3,StATG4,StATG5,StATG7 and StATG16 genes for autophagy were induced by ATR-mediated S-phase checkpoint.We therefore propose that in response to genotoxic stress,S.turcica utilizes ATR-dependent pathway to turn off transcription of genes governing appressorium-mediated infection,and meanwhile inducing transcription of autophagy genes likely as a mechanism of self-protection,aside from the more conservative responses in eukaryotes.
基金National Research and Development Project of China,Grant/Award Number:2022YFC0867600CAMS initiative for Innovative Medicine of China,Grant/Award Number:2021-I2M-1-035。
文摘Background:New Omicron subvariants are emerging rapidly from BA.1 to BA.4 and BA.5.Their pathogenicity has changed from that of wild-type(WH-09)and Omicron variants have over time become globally dominant.The spike proteins of BA.4 and BA.5 that serve as the target for vaccine-induced neutralizing antibodies have also changed compared to the previous subvariants,which is likely to cause immune es-cape and the reduction of the protective effect of the vaccine.Our study addresses the above issues and provides a basis for formulating relevant prevention and control strategies.Methods:We collected cellular supernatant and cell lysates and measured the viral titers,viral RNA loads,and E subgenomic RNA(E sgRNA)loads in different Omicron subvariants grown in Vero E6 cells,using WH-09 and Delta variants as a reference.Additionally,we evaluated the in vitro neutralizing activity of different Omicron sub-variants and compared it to the WH-09 and Delta variants using macaque sera with different types of immunity.Results:As the SARS-CoV-2 evolved into Omicron BA.1,the replication ability in vitro began to decrease.Then with the emergence of new subvariants,the replication ability gradually recovered and became stable in the BA.4 and BA.5 subvariants.In WH-09-inactivated vaccine sera,geometric mean titers of neutralization antibodies against different Omicron subvariants declined by 3.7~15.4-fold compared to those against WH-09.In Delta-inactivated vaccine sera,geometric mean titers of neutrali-zation antibodies against Omicron subvariants declined by 3.1~7.4-fold compared to those against Delta.Conclusion:According to the findings of this research,the replication efficiency of all Omicron subvariants declined compared with WH-09 and Delta variants,and was lower in BA.1 than in other Omicron subvariants.After two doses of inactivated(WH-09 or Delta)vaccine,cross-neutralizing activities against various Omicron subvariants were seen despite a decline in neutralizing titers.
文摘The impact of a Distributed Denial of Service(DDoS)attack on Soft-ware Defined Networks(SDN)is briefly analyzed.Many approaches to detecting DDoS attacks exist,varying on the feature being considered and the method used.Still,the methods have a deficiency in the performance of detecting DDoS attacks and mitigating them.To improve the performance of SDN,an efficient Real-time Multi-Constrained Adaptive Replication and Traffic Approximation Model(RMCARTAM)is sketched in this article.The RMCARTAM considers different parameters or constraints in running different controllers responsible for handling incoming packets.The model is designed with multiple controllers to handle net-work traffic but can turn the controllers according to requirements.The multi-con-straint adaptive replication model monitors different features of network traffic like rate of packet reception,class-based packet reception and target-specific reception.According to these features,the method estimates the Replication Turn-ing Weight(RTW)based on which triggering controllers are performed.Similarly,the method applies Traffic Approximation(TA)in the detection of DDoS attacks.The detection of a DDoS attack is performed by approximating the incoming traf-fic to any service and using various features like hop count,payload,service fre-quency,and malformed frequency to compute various support measures on bandwidth access,data support,frequency support,malformed support,route sup-port,and so on.Using all these support measures,the method computes the value of legitimate weight to conclude the behavior of any source in identifying the mal-icious node.Identified node details are used in the mitigation of DDoS attacks.The method stimulates the network performance by reducing the power factor by switching the controller according to different factors,which also reduces the cost.In the same way,the proposed model improves the accuracy of detecting DDoS attacks by estimating the features of incoming traffic in different corners.
文摘Each rock joint is unique by nature which means that utilization of replicas in direct shear tests is required in experimental parameter studies.However,a method to acquire knowledge about the ability of the replicas to imitate the shear mechanical behavior of the rock joint and their dispersion in direct shear testing is lacking.In this study,a novel method is presented for geometric quality assurance of replicas.The aim is to facilitate generation of high-quality direct shear testing data as a prerequisite for reliable subsequent analyses of the results.In Part 1 of this study,two quality assurance parameters,smf and V_(Hp100),are derived and their usefulness for evaluation of geometric deviations,i.e.geometric reproducibility,is shown.In Part 2,the parameters are validated by showing a correlation between the parameters and the shear mechanical behavior,which qualifies the parameters for usage in the quality assurance method.Unique results from direct shear tests presenting comparisons between replicas and the rock joint show that replicas fulfilling proposed threshold values of σ_(mf)<0.06 mm and|V_(Hp100)|<0.2 mm have a narrow dispersion and imitate the shear mechanical behavior of the rock joint in all aspects apart from having a slightly lower peak shear strength.The wear in these replicas,which have similar morphology as the rock joint,is in the same areas as in the rock joint.The wear is slightly larger in the rock joint and therefore the discrepancy in peak shear strength derives from differences in material properties,possibly from differences in toughness.It is shown by application of the suggested method that the quality assured replicas manufactured following the process employed in this study phenomenologically capture the shear strength characteristics,which makes them useful in parameter studies.
基金supported by the Deanship of Scientific Research at Prince Sattam Bin Abdulaziz University under research Project No.2018/01/9371.
文摘The reliability and availability of cloud systems have become major concerns of service providers,brokers,and end-users.Therefore,studying fault-tolerance mechanisms in cloud computing attracts intense attention in industry and academia.The task-scheduling mechanisms can improve the fault-tolerance level of cloud systems.A task-scheduling mechanism distributes tasks to a group of instances to be executed.Much work has been undertaken in this direction to improve the overall outcome of cloud computing,such as improving service qual-ity and reducing power consumption.However,little work on task scheduling has studied the problem of lost tasks from the broker’s perspective.Task loss can hap-pen due to virtual machine failures,server crashes,connection interruption,etc.The broker-based concept means that the backup task can be allocated by the bro-ker on the same cloud service provider(CSP)or a different CSP to reduce costs,for example.This paper proposes a novel fault-tolerant mechanism that employs the primary backup(PB)model of task scheduling to address this issue.The pro-posed mechanism minimizes the impact of failure events by reducing the number of lost tasks.The mechanism is further improved to shorten the makespan time of submitted tasks in cloud systems.The experiments demonstrated that the pro-posed mechanism decreased the number of lost tasks by about 13%–15%com-pared with other mechanisms in the literature.
文摘Many cutting-edge methods are now possible in real-time commercial settings and are growing in popularity on cloud platforms.By incorporating new,cutting-edge technologies to a larger extent without using more infrastructures,the information technology platform is anticipating a completely new level of devel-opment.The following concepts are proposed in this research paper:1)A reliable authentication method Data replication that is optimised;graph-based data encryp-tion and packing colouring in Redundant Array of Independent Disks(RAID)sto-rage.At the data centre,data is encrypted using crypto keys called Key Streams.These keys are produced using the packing colouring method in the web graph’s jump graph.In order to achieve space efficiency,the replication is carried out on optimised many servers employing packing colours.It would be thought that more connections would provide better authentication.This study provides an innovative architecture with robust security,enhanced authentication,and low cost.
文摘Most social networks allow connections amongst many people based on shared interests.Social networks have to offer shared data like videos,photos with minimum latency to the group,which could be challenging as the storage cost has to be minimized and hence entire data replication is not a solution.The replication of data across a network of read-intensive can potentially lead to increased savings in cost and energy and reduce the end-user’s response time.Though simple and adaptive replication strategies exist,the solution is non-deter-ministic;the replicas of the data need to be optimized to the data usability,perfor-mance,and stability of the application systems.To resolve the non-deterministic issue of replication,metaheuristics are applied.In this work,Harmony Search and Tabu Search algorithms are used optimizing the replication process.A novel Har-mony-Tabu search is proposed for effective placement and replication of data.Experiments on large datasets show the effectiveness of the proposed technique.It is seen that the bandwidth saving for proposed harmony-Tabu replication per-forms better in the range of 3.57%to 18.18%for varying number of cloud data-centers when compared to simple replication,Tabu replication and Harmony replication algorithm.
基金Supported by Guangzhou Science and Technology Plan(201804010327,202002020040,202102080340)Agricultural Science-Technology Innovation and Promotion Project(2023KJ133)。
文摘Tomato yellow leaf curl virus(TYLCV)is the dominating pathogen of tomato yellow leaf curl disease that caused severe loss to tomato production in China.In this study,we found that a TYLCV-resistant tomato line drastically reduced the accumulation of viral complementary-sense strand mRNAs but just moderately inhibited that of viral DNA and virion-sense strand mRNAs.However,two other resistant lines did not have such virus inhibition pattern.Analysis of differential expressed genes showed that the potential host defense-relevant processes varied in different resistant tomatoes,as compared to the susceptible line,suggesting a diversity of tomato TYLCV-resistance mechanisms.
文摘Memetics confirms the importance of recitation and imitation in college English teaching.Starting with the explanation of the replication cycle of language memes,this paper discusses how to use the memetic perspective in college English listening and speaking teaching,effectively transform the input language information into language output,and improve the classroom effect of college English listening and speaking teaching.
基金supported by National Nature Science Foundation of China(82172978)Taishan Scholars Program of Shandong Province(Grant No.tsqn201909147)+1 种基金the Key Project at Central Government Level:the ability establishment of sustainable use for valuable Chinese medicine resources(2060302)the Student Innovation Training Program in Jining Medical University(cx2021116).
文摘Background:This study aimed to select compounds with unique inhibitory effects on muscle-invasive bladder cancer(MIBC)from coumarone derivatives with similar parent nuclear structures and to reveal their tumor-suppressive effects using various approaches.Methods:Bladder cancer cell lines SW780 and T24,as well as human normal bladder epithelial cell line SV-HUC-1 were selected as the study model,and these urinary system cells were co-incubated with various concentrations of(S,E)-4-(4-methylbenzylidene)-3-phenylchroman-3-ol,(S,E)-4-(4-isocyanobenzylidene)-3-phenylchroman-3-ol,(S,E)-4-(4-fluorobenzylidene)-3-phenylchroman-3-ol(FPO),and(S,E)-3-phenyl-4-(4-(trifluoromethoxy)benzylidene)chroman-3-ol.Cell activity was detected using cell counting kit-8.FPO showed the strongest inhibitory effect on MIBC cells;therefore,it was selected for further experiments.We monitored the FPO-induced T24 cell morphological changes with an inverted microscope.The FPO-inhibited migration of T24 cells was examined using a cell scratch assay.We detected the clonogenic ability of T24 cells through a clone formation test and evaluated their proliferative ability using a 5-ethynyl-2’-deoxyuridine fluorescence staining kit.The inhibitory effect of FPO against the cell cycle was monitored using flow cytometry,and its suppressive effect on the DNA replication ability of T24 cells was detected using double fluorescence staining(Ki67 and phalloidin).Results:Among the four candidate coumarone derivatives,FPO showed the most significant inhibitory effect on MIBC cells and was less toxic to normal urothelial cells.FPO inhibited T24 cell growth in time and dose-dependent manners(the half-inhibitory concentration is 8μM).FPO significantly repressed the proliferation,migration,and clonogenic ability of bladder cancer T24 cells.Cell mobility was significantly inhibited by FPO:30μM FPO almost completely repressed migration occurred at after 24 h treatment.Moreover,FPO significantly suppressed the clonogenicity of bladder cancer cells in a dose-dependent manner.Mechanistically,FPO targeted the cell cycle,arresting the S and G2 phases on bladder cancer T24 cells.Conclusion:We discovered a novel anticancer chemical,FPO,and proposed a potential mechanism,through which it suppresses MIBC T24 cells by repressing the cell cycle in the S and G2 phases.This study contributes to the development of novel anticancer drugs for MIBC.
