Objective: Observing the expression changes of serum proteome in model rats after intervention of the Granules of Eliminating Phlegm and Removing Blood Stasis (豁痰祛瘀颗粒 also known as GEPRB), screening out and iden...Objective: Observing the expression changes of serum proteome in model rats after intervention of the Granules of Eliminating Phlegm and Removing Blood Stasis (豁痰祛瘀颗粒 also known as GEPRB), screening out and identifying the differentially expressed proteins by mass spectrometry and bioinformatics analysis, discussing the molecular mechanism of control the Diabetes deafness by GEPRB. Methods: By use of proteomics technology, the serum protein serum proteome of the control group, model control group, Duxil and each observation group were observed for 2-DE gel pattern matching, and the difference in the relative content of 2 times was chosen for the differentially expressed proteins. Identification of differentially expressed proteins by MALDI-TOF MS/MS, the authors further analysis the phosphorylation, subcellular localization, interaction, direct regulation, and transmembrane of the differences proteins by the way of bioinformatics analysis. Sixty SPF level SD rats elected in diabetic rats model group (abbreviated as DM group) were be randomly divided into 5 groups based on random number sheet, namely model control group, positive drug control group (Du-ke-xi group) and Mai-tong-fang high, medium and low dose group respectively. In addition, set of normal control group. 10 rats in each group. Results: By Coomassie brilliant blue staining, identified 51 differential protein spots dug from 2-D gel by mass spectrometry, successfully identified 13 non-redundant proteins. Most of the identified proteins were secreted protein and belong to different protein families. There were about 12 proteins have the transmembrane region from the authors’ result, ten of them were plasma membrane proteins. Conclusion: It’s suggesting that 13 differential proteins is most likely the protein response to GEPRB in vivo, these proteins may play key role for the treatment of GEPRB to Diabetes deafness. The two highly differentially expressed proteins Apolipoprotein E (apoE) and C3 may be a potential drug target of GEPRB.展开更多
目的:观察化痰散瘀汤治疗斑块型银屑病(痰瘀互结证)的临床疗效及临床作用机制。方法:将皮肤科斑块型银屑病(痰瘀互结证)患者80例按随机数字表法分为观察组与对照组各40例。对照组予阿维A胶囊+糠酸莫米松软膏治疗,观察组在此基础上予化...目的:观察化痰散瘀汤治疗斑块型银屑病(痰瘀互结证)的临床疗效及临床作用机制。方法:将皮肤科斑块型银屑病(痰瘀互结证)患者80例按随机数字表法分为观察组与对照组各40例。对照组予阿维A胶囊+糠酸莫米松软膏治疗,观察组在此基础上予化痰散瘀汤治疗。观察两组患者中医证候评分(皮损颜色、皮损肥厚浸润、瘙痒、肌肤甲错),银屑病皮损面积和严重程度指数(psoriasis area and severity index,PASI)评分,皮肤病生活质量指数调查表(dermatology life quality index,DLQI)评分,血清免疫细胞因子干扰素γ(interferonγ,IFN-γ)、白细胞介素4(interleukin 4,IL-4)、白细胞介素17A(interleukin 17A,IL-17A)、白细胞介素10(interleukin-10,IL-10)、白细胞介素22(interleukin-22,IL-22)改善状况,并统计临床疗效及不良反应发生情况。结果:两组治疗后皮损颜色、皮损肥厚浸润、瘙痒、肌肤甲错积分均明显降低(P<0.05),且观察组治疗后中医证候积分明显低于对照组(P<0.05)。两组治疗后PASI及DLQI评分均明显降低(P<0.05),且观察组明显低于对照组(P<0.05)。两组治疗后血清IFN-γ、IL-17A、IL-22水平均明显降低(P<0.05),IL-4及IL-10水平均明显升高(P<0.05),且观察组上述指标改善情况均优于对照组(P<0.05)。观察组总有效率[92.5%(37/40)]明显高于对照组[75.0%(30/40)](P<0.05)。两组治疗期间均未出现明显不良反应。结论:化痰散瘀汤能有效改善斑块型银屑病(痰瘀互结证)患者皮损状况,缓解瘙痒等临床症状,有助于提高生活质量,其可能与调节IFN-γ、IL-4、IL-17A、IL-10、IL-22等免疫细胞因子,纠正患者机体紊乱的免疫机能有关。展开更多
文摘Objective: Observing the expression changes of serum proteome in model rats after intervention of the Granules of Eliminating Phlegm and Removing Blood Stasis (豁痰祛瘀颗粒 also known as GEPRB), screening out and identifying the differentially expressed proteins by mass spectrometry and bioinformatics analysis, discussing the molecular mechanism of control the Diabetes deafness by GEPRB. Methods: By use of proteomics technology, the serum protein serum proteome of the control group, model control group, Duxil and each observation group were observed for 2-DE gel pattern matching, and the difference in the relative content of 2 times was chosen for the differentially expressed proteins. Identification of differentially expressed proteins by MALDI-TOF MS/MS, the authors further analysis the phosphorylation, subcellular localization, interaction, direct regulation, and transmembrane of the differences proteins by the way of bioinformatics analysis. Sixty SPF level SD rats elected in diabetic rats model group (abbreviated as DM group) were be randomly divided into 5 groups based on random number sheet, namely model control group, positive drug control group (Du-ke-xi group) and Mai-tong-fang high, medium and low dose group respectively. In addition, set of normal control group. 10 rats in each group. Results: By Coomassie brilliant blue staining, identified 51 differential protein spots dug from 2-D gel by mass spectrometry, successfully identified 13 non-redundant proteins. Most of the identified proteins were secreted protein and belong to different protein families. There were about 12 proteins have the transmembrane region from the authors’ result, ten of them were plasma membrane proteins. Conclusion: It’s suggesting that 13 differential proteins is most likely the protein response to GEPRB in vivo, these proteins may play key role for the treatment of GEPRB to Diabetes deafness. The two highly differentially expressed proteins Apolipoprotein E (apoE) and C3 may be a potential drug target of GEPRB.
文摘目的:观察化痰散瘀汤治疗斑块型银屑病(痰瘀互结证)的临床疗效及临床作用机制。方法:将皮肤科斑块型银屑病(痰瘀互结证)患者80例按随机数字表法分为观察组与对照组各40例。对照组予阿维A胶囊+糠酸莫米松软膏治疗,观察组在此基础上予化痰散瘀汤治疗。观察两组患者中医证候评分(皮损颜色、皮损肥厚浸润、瘙痒、肌肤甲错),银屑病皮损面积和严重程度指数(psoriasis area and severity index,PASI)评分,皮肤病生活质量指数调查表(dermatology life quality index,DLQI)评分,血清免疫细胞因子干扰素γ(interferonγ,IFN-γ)、白细胞介素4(interleukin 4,IL-4)、白细胞介素17A(interleukin 17A,IL-17A)、白细胞介素10(interleukin-10,IL-10)、白细胞介素22(interleukin-22,IL-22)改善状况,并统计临床疗效及不良反应发生情况。结果:两组治疗后皮损颜色、皮损肥厚浸润、瘙痒、肌肤甲错积分均明显降低(P<0.05),且观察组治疗后中医证候积分明显低于对照组(P<0.05)。两组治疗后PASI及DLQI评分均明显降低(P<0.05),且观察组明显低于对照组(P<0.05)。两组治疗后血清IFN-γ、IL-17A、IL-22水平均明显降低(P<0.05),IL-4及IL-10水平均明显升高(P<0.05),且观察组上述指标改善情况均优于对照组(P<0.05)。观察组总有效率[92.5%(37/40)]明显高于对照组[75.0%(30/40)](P<0.05)。两组治疗期间均未出现明显不良反应。结论:化痰散瘀汤能有效改善斑块型银屑病(痰瘀互结证)患者皮损状况,缓解瘙痒等临床症状,有助于提高生活质量,其可能与调节IFN-γ、IL-4、IL-17A、IL-10、IL-22等免疫细胞因子,纠正患者机体紊乱的免疫机能有关。
