Objective:The Box-Behnken response surface method combined with fingerprints was used to optimize the extraction process of total anthraquinone from Cassia seeds.Methods:A three-factor,three-level response surface tes...Objective:The Box-Behnken response surface method combined with fingerprints was used to optimize the extraction process of total anthraquinone from Cassia seeds.Methods:A three-factor,three-level response surface test was conducted based on the single-factor test with comprehensive evaluation as the measurement index.The comprehensive evaluation indexes included the extraction rate of total anthraquinone of Cassia seeds or the equivalent amount of herbs per gram of total anthraquinone of Cassia seeds,the normalized value of peak areas of 5 index components such as aurantio obtusin in the fingerprint of each sample to 16 shared peaks and the similarity of fingerprints(the reference fingerprint was established by the extraction solvent for the determination of Cassia seeds content in the Chinese Pharmacopoeia 2020 edition)with the weights of 0.2,0.5 and 0.3,respectively.Results:The best extraction process was obtained:the liquid-to-material ratio was 20:1(mL·g-1),the extraction solvent was mixture of 60%ethanol-ethyl acetate(2:1),and the extraction time was 15.12 min.The results of five sets of validation experiments showed that the overall evaluation of total anthraquinone of cassia seeds by the best process was 0.528(RSD=0.45%),and the prediction result of response surface method model was 0.531,and the relative error with the prediction result was 0.531.The relative error of the predicted results was 0.56%,and the best extraction process was consistent with the model prediction,and the obtained best process could be used for the extraction of total anthraquinone from Cassia seeds.Conclusion:The Box-Behnken response surface method combined with the fingerprint technique can be used to find the best reaction conditions and examine the interactions among the factors in a comprehensive and accurate manner,which can provide reference for the optimization and evaluation of the extraction process of Chinese medicine.展开更多
[Objectives]This study was conducted to confirm the existence of anthocyanins in pitaya(Hylocereus undatus)pericarp and optimize the extraction condition.[Methods]Single-factor tests were carried out on five factors:e...[Objectives]This study was conducted to confirm the existence of anthocyanins in pitaya(Hylocereus undatus)pericarp and optimize the extraction condition.[Methods]Single-factor tests were carried out on five factors:ethanol concentration,extraction temperature,ultrasonic power,solid-to-liquid ratio and extraction time.Based on the single-factor test results,four factors and three levels of process conditions were optimized by Box-Behnken design test.[Results]Anthocyanins exist in the pericarp of pitaya,and the optimum conditions were extraction temperature 49℃,ultrasonic power 120 W,extracted time 2 h and ethanol concentration 60%.Under these conditions,the average yield of anthocyanins was 80 mg/100g.[Conclusions]This study provides a theoretical basis for the extraction of anthocyanins from the pericarp of red-flesh pitaya.展开更多
In seed processing for commercial production,the collision or friction between mechanical parts and seed is inevitable.These collisions or friction can cause cracks on the surface of the seed,which can affect germinat...In seed processing for commercial production,the collision or friction between mechanical parts and seed is inevitable.These collisions or friction can cause cracks on the surface of the seed,which can affect germination rates and ultimately reduce crop yields.Using the difference of the seed motion trajectories with different crack degrees in the magnetic field,the grading of the surface crack seed can be realized.In this study,the motion law of seed in a non-uniform magnetic field was analyzed by taking the delinted cottonseed as an object.A magnetic separation device for crop seeds was developed.This device was essentially composed of the feed throat,magnetic roller,conveyor belt,and variable-frequency adjustable-speed motor.The magnetic powder adhering seeds enter the magnetic separation device through the feed throat and are conveyed to the magnetic roller through the conveyor belt.The magnetic roller has different adsorption forces on seeds with different degrees of cracking,and seeds fall into different outlets,thus completing the grading of the seeds.By adjusting working parameters such as the magnetic field strength and the speed of the conveyor belt,magnetic separation could be made.In order to verify the grading effect,the magnetic separation accuracy was taken as the inspection index,the magnetic powder’s mesh number,the mass mixing ratio between magnetic powder and delinted cottonseed,and the rotation speed of the magnetic roller were taken as the inspection factors.The response surface methodology was used to optimize the working parameters of the experimental device.The results showed that the optimal process parameters of the magnetic separation device were as follows:the number of magnetic powder meshes was 250,the mass mixing ratio between magnetic powder and delinted cottonseed was 1:20,and when the rotational speed of the magnetic separation roller was 20 r/min,the detection rate reached 92.5%.The designed magnetic separation device can realize the non-destructive batch detection of seed surface cracks with high work efficiency and has guiding significance for the high-precision and low-damage classification detection and classification of commercial seed.展开更多
基金2018 Anhui Provincial Quality Engineering Project(No.2018jyxm1273)Major Provincial Natural Science Research Project of Anhui Universities(No.KJ2016SD60)2021 Anhui University Students’Innovation and Entrepreneurship Plan Project。
文摘Objective:The Box-Behnken response surface method combined with fingerprints was used to optimize the extraction process of total anthraquinone from Cassia seeds.Methods:A three-factor,three-level response surface test was conducted based on the single-factor test with comprehensive evaluation as the measurement index.The comprehensive evaluation indexes included the extraction rate of total anthraquinone of Cassia seeds or the equivalent amount of herbs per gram of total anthraquinone of Cassia seeds,the normalized value of peak areas of 5 index components such as aurantio obtusin in the fingerprint of each sample to 16 shared peaks and the similarity of fingerprints(the reference fingerprint was established by the extraction solvent for the determination of Cassia seeds content in the Chinese Pharmacopoeia 2020 edition)with the weights of 0.2,0.5 and 0.3,respectively.Results:The best extraction process was obtained:the liquid-to-material ratio was 20:1(mL·g-1),the extraction solvent was mixture of 60%ethanol-ethyl acetate(2:1),and the extraction time was 15.12 min.The results of five sets of validation experiments showed that the overall evaluation of total anthraquinone of cassia seeds by the best process was 0.528(RSD=0.45%),and the prediction result of response surface method model was 0.531,and the relative error with the prediction result was 0.531.The relative error of the predicted results was 0.56%,and the best extraction process was consistent with the model prediction,and the obtained best process could be used for the extraction of total anthraquinone from Cassia seeds.Conclusion:The Box-Behnken response surface method combined with the fingerprint technique can be used to find the best reaction conditions and examine the interactions among the factors in a comprehensive and accurate manner,which can provide reference for the optimization and evaluation of the extraction process of Chinese medicine.
基金Supported by Food Science Innovation Team Project of Guangdong Colleges and Universities(2016KCXTD020)Guangdong Science and Technology Planning Project(2014B090908007).
文摘[Objectives]This study was conducted to confirm the existence of anthocyanins in pitaya(Hylocereus undatus)pericarp and optimize the extraction condition.[Methods]Single-factor tests were carried out on five factors:ethanol concentration,extraction temperature,ultrasonic power,solid-to-liquid ratio and extraction time.Based on the single-factor test results,four factors and three levels of process conditions were optimized by Box-Behnken design test.[Results]Anthocyanins exist in the pericarp of pitaya,and the optimum conditions were extraction temperature 49℃,ultrasonic power 120 W,extracted time 2 h and ethanol concentration 60%.Under these conditions,the average yield of anthocyanins was 80 mg/100g.[Conclusions]This study provides a theoretical basis for the extraction of anthocyanins from the pericarp of red-flesh pitaya.
基金supported by the National Key R&D Program of China (Grant No.2018YFD0101000)National Natural Science Foundation of China (Grant No.52075150)Innovation Scientists and Technicians Team Projects of Henan Provincial Department of Education (23IRTSTHN015).
文摘In seed processing for commercial production,the collision or friction between mechanical parts and seed is inevitable.These collisions or friction can cause cracks on the surface of the seed,which can affect germination rates and ultimately reduce crop yields.Using the difference of the seed motion trajectories with different crack degrees in the magnetic field,the grading of the surface crack seed can be realized.In this study,the motion law of seed in a non-uniform magnetic field was analyzed by taking the delinted cottonseed as an object.A magnetic separation device for crop seeds was developed.This device was essentially composed of the feed throat,magnetic roller,conveyor belt,and variable-frequency adjustable-speed motor.The magnetic powder adhering seeds enter the magnetic separation device through the feed throat and are conveyed to the magnetic roller through the conveyor belt.The magnetic roller has different adsorption forces on seeds with different degrees of cracking,and seeds fall into different outlets,thus completing the grading of the seeds.By adjusting working parameters such as the magnetic field strength and the speed of the conveyor belt,magnetic separation could be made.In order to verify the grading effect,the magnetic separation accuracy was taken as the inspection index,the magnetic powder’s mesh number,the mass mixing ratio between magnetic powder and delinted cottonseed,and the rotation speed of the magnetic roller were taken as the inspection factors.The response surface methodology was used to optimize the working parameters of the experimental device.The results showed that the optimal process parameters of the magnetic separation device were as follows:the number of magnetic powder meshes was 250,the mass mixing ratio between magnetic powder and delinted cottonseed was 1:20,and when the rotational speed of the magnetic separation roller was 20 r/min,the detection rate reached 92.5%.The designed magnetic separation device can realize the non-destructive batch detection of seed surface cracks with high work efficiency and has guiding significance for the high-precision and low-damage classification detection and classification of commercial seed.
