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Resilient performance of self-centering hybrid rocking walls with curved interface under pseudo-static loading
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作者 Su Xing Yan Shi +1 位作者 Sun Xianglei Wang Tao 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2024年第1期65-85,共21页
Frame and rocking wall(FRW)structures have excellent resilient performance during earthquakes.However,the concrete at interfacial corners of rocking walls(RWs)is easily crushed due to local extreme compression during ... Frame and rocking wall(FRW)structures have excellent resilient performance during earthquakes.However,the concrete at interfacial corners of rocking walls(RWs)is easily crushed due to local extreme compression during the rocking process.An innovative RW with a curved interface is proposed to prevent interfacial corners from producing local damage,enhancing its earthquake resilient performance(ERP).The precast wall panel with a curved interface is assembled into an integral self-centering hybrid rocking wall(SCRW)by two post-tensioned unbonded prestressed tendons.Moreover,two ordinary energy dissipation steel rebars and two shear reinforcements are arranged to increase the energy dissipation capacity and lateral resistance.Two SCRW specimens and one monolithic reinforced concrete(RC)shear wall(SW)were tested under pseudo-static loading to compare the ERPs of the proposed SCRW and the SW,focusing on studying the effect of the curved interface on the SCRW.The key resilient performance of rocking effects,failure modes,and hysteretic properties of the SCRW were explored.The results show that nonlinear deformations of the SCRW are concentrated along the interface between the SCRW and the foundation,avoiding damage within the SCRW.The restoring force provided by the prestressed tendons can effectively realize self-centering capacity with small residual deformation,and the resilient performance of the SCRW is better than that of monolithic SW.In addition,the curved interface of the SCRW makes the rocking center change and move inward,partially relieving the stress concentration and crush of concrete.The rocking range of the rocking center is about 41.4%of the width of the SCRW. 展开更多
关键词 self-centering hybrid rocking wall(SCRW) monolithic shear wall(SW) earthquake resilient performance(ERP) curved interface rocking center
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Pulses in ground motions identified through surface partial matching and their impact on seismic rocking consequence
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作者 Tang Yuchuan Wang Jiankang Wu Gang 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2024年第1期35-50,共16页
In seismology and earthquake engineering,it is fundamental to identify and characterize the pulse-like features in pulse-type ground motions.To capture the pulses that dominate structural responses,this study establis... In seismology and earthquake engineering,it is fundamental to identify and characterize the pulse-like features in pulse-type ground motions.To capture the pulses that dominate structural responses,this study establishes congruence and shift relationships between response spectrum surfaces.A similarity search between spectrum surfaces,supplemented with a similarity search in time series,has been applied to characterize the pulse-like features in pulse-type ground motions.The identified pulses are tested in predicting the rocking consequences of slender rectangular blocks under the original ground motions.Generally,the prediction is promising for the majority of the ground motions where the dominant pulse is correctly identified. 展开更多
关键词 velocity pulse ground motion surface similarity rocking OVERTURNING
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Anti-interference self-alignment algorithm by attitude optimization estimation for SINS on a rocking base
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作者 XUE Haijian WANG Tao +2 位作者 CAI Xinghui WANG Jintao LIU Fei 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2023年第5期1333-1342,共10页
The performance of a strapdown inertial navigation system(SINS)largely depends on the accuracy and rapidness of the initial alignment.A novel anti-interference self-alignment algorithm by attitude optimization estimat... The performance of a strapdown inertial navigation system(SINS)largely depends on the accuracy and rapidness of the initial alignment.A novel anti-interference self-alignment algorithm by attitude optimization estimation for SINS on a rocking base is presented in this paper.The algorithm transforms the initial alignment into the initial attitude determination problem by using infinite vector observations to remove the angular motions,the SINS alignment is heuristically established as an optimiza-tion problem of finding the minimum eigenvector.In order to further improve the alignment precision,an adaptive recursive weighted least squares(ARWLS)curve fitting algorithm is used to fit the translational motion interference-contaminated reference vectors according to their time domain characteristics.Simulation studies and experimental results favorably demonstrate its rapidness,accuracy and robustness. 展开更多
关键词 strapdown inertial navigation system(SINS) initial alignment ANTI-INTERFERENCE rocking base adaptive recursive weighted least squares(ARWLS)
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Sn-doped BiOCl nanosheet with synergistic H^(+)/Zn^(2+)co-insertion for“rocking chair”zinc-ion battery
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作者 Yuzhu Qian Hongrui Wang +6 位作者 Xinni Li Ting Song Yong Pei Li Liu Bei Long Xiongwei Wu Xianyou Wang 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2023年第6期623-632,I0014,共11页
The development of insertion-type anodes is the key to designing“rocking chair”zinc-ion batteries.However,there is rare report on high mass loading anode with high performances.Here,{001}-oriented Bi OCl nanosheets ... The development of insertion-type anodes is the key to designing“rocking chair”zinc-ion batteries.However,there is rare report on high mass loading anode with high performances.Here,{001}-oriented Bi OCl nanosheets with Sn doping are proposed as a promising insertion-type anode.The designs of cross-linked CNTs conductive network,{001}-oriented nanosheet,and Sn doping significantly enhance ion/electron transport,proved via experimental tests and theoretical calculations(density of states and diffusion barrier).The H^(+)/Zn^(2+)synergistic co-insertion mechanism is proved via ex situ XRD,Raman,XPS,and SEM tests.Accordingly,this optimized electrode delivers a high reversible capacity of 194 m A h g^(-1)at 0.1 A g^(-1)with a voltage of≈0.37 V and an impressive cyclability with 128 m A h g^(-1)over 2500 cycles at 1 A g^(-1).It also shows satisfactory performances at an ultrahigh mass loading of 10 mg cm^(-2).Moreover,the Sn-Bi OCl//MnO_(2)full cell displays a reversible capacity of 85 m A h g^(-1)at 0.2 A g^(-1)during cyclic test. 展开更多
关键词 Sn-doped BiOCl nanosheet High mass loading anode Synergistic H^(+)/Zn^(2+)co-insertion Fast ionic/electronic diffusion rocking chair”zinc-ion battery
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A discretely damped SDOF model for the rocking response of freestanding blocks
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作者 Liu Hanquan Huang Yuli Qu Zhe 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2022年第3期729-740,共12页
This paper presents a single-degree-of-freedom(SDOF)constitutive model for assessing the performance of freestanding block contents of buildings.The model incorporates a bespoke damper to account for energy dissipatio... This paper presents a single-degree-of-freedom(SDOF)constitutive model for assessing the performance of freestanding block contents of buildings.The model incorporates a bespoke damper to account for energy dissipation associated with rocking.It is advantageous in its direct correlation,via energy conservation,to the restitution coefficient for impact during rocking.A comparative study with the existing SDOF rocking models shows that the proposed model significantly improves the accuracy of free-rocking simulations,in which inherent damping predominantly affects response.It provides a promising and efficient tool for computationally intensive performance evaluation of nonstructural components. 展开更多
关键词 CONTENTS rigid block equivalent viscous damping free rocking forced rocking single-degree-of-freedom system
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Analytical evaluation and experimental validation on dynamic rocking behavior for shallow foundation considering structural response
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作者 Kil-Wan Ko Jeong-Gon Ha Dong-Soo Kim 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2022年第1期37-51,共15页
The challenge in the practical application of rocking foundations is the estimation of its performance,particularly the rotation angle,during a strong earthquake.In this study,the dynamic rocking behavior for a shallo... The challenge in the practical application of rocking foundations is the estimation of its performance,particularly the rotation angle,during a strong earthquake.In this study,the dynamic rocking behavior for a shallow foundation considering structural response was evaluated through two analytical approaches:the conventional soil-foundation-structure interaction(SFSI)governing equation of a single-degree-of-freedom(SDOF)structure on a rocking shallow foundation,and the Housner rocking model(i.e.,a rocking rigid block on a rigid base).Both approaches were validated with dynamic centrifuge tests.The test models consisted of a soft soil deposit,a shallow rectangular foundation,and an SDOF structure dominated by a bending behavior.A total of 11 foundation-structure systems and six seismic waves,including recorded earthquake signals and sinusoidal waves,were utilized.The results showed that the conventional SFSI equation well predicted the maximum rotation during strong earthquakes.However,this method was less accurate regarding the rotational phase information and maximum rotation of the foundation during weak earthquakes.On the other hand,although the modified Housner′s rocking model required five parameters relevant to a soil-foundation-structure system,it overestimated the maximum rotation of the foundation when compared with the results from dynamic centrifuge tests. 展开更多
关键词 soil-foundation-structure interaction maximum rotation angle rocking foundation Housner rocking model dynamic centrifuge test
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Rocking Block线碰撞离散化研究 被引量:2
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作者 张宏剑 庄方方 +2 位作者 王辰 季宝锋 石玉红 《动力学与控制学报》 2017年第4期330-334,共5页
针对Rocking Block中的线碰撞问题,首先采用离散化思想将线碰撞问题离散为多点碰撞系统,而后基于LZB方法对所建多点碰撞系统进行动力学建模.仿真结果表明随着离散点数的增加,基于LZB方法的多点碰撞模型能够很好地刻画Rocking Block中的... 针对Rocking Block中的线碰撞问题,首先采用离散化思想将线碰撞问题离散为多点碰撞系统,而后基于LZB方法对所建多点碰撞系统进行动力学建模.仿真结果表明随着离散点数的增加,基于LZB方法的多点碰撞模型能够很好地刻画Rocking Block中的相关线碰撞问题,且精度与离散程度紧密相关. 展开更多
关键词 线碰撞 多点碰撞 非光滑动力学 rocking BLOCK
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Controlled rocking pile foundation system with replaceable bar fuses for seismic resilience 被引量:2
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作者 Cheng Zhao Li Shuai Wang Jingquan 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2022年第3期683-696,共14页
Bridges designed following a conventional approach minimize the risk of collapse,but often require challenging,costly,and time-consuming restoration after an earthquake occurs.The new seismic design philosophy require... Bridges designed following a conventional approach minimize the risk of collapse,but often require challenging,costly,and time-consuming restoration after an earthquake occurs.The new seismic design philosophy requires bridges to maintain functionality even after severe earthquakes.In this context,this paper proposes a controlled rocking pile foundation(CRPF)system and numerically evaluates bridges′degree of seismic resilience.The CRPF system allows a pile cap to rock on a pile foundation and dissipate seismic energy through inelastic deformations of replaceable bar fuses that connect a pile cap and piles.Following the conceptual design of the CRPF system,two analytical models were developed for a bridge pier utilizing the CRPF system and a pier designed to develop a plastic hinge in its column.The analytical results indicate that,after experiencing a severe earthquake,a conventionally designed bridge pier sustained substantial damage in its column and exhibited significant residual displacement.In contrast,a pier using the CRPF system showed negligible residual displacement and maintained elastic behavior except,as expected,for bar fuses.The damaged fuses can be rapidly replaced to recover bridge seismic resistance following an earthquake.Therefore,the CRPF system helps to achieve the desired postearthquake performance objectives. 展开更多
关键词 seismic resilience bridges pier controlled rocking pile foundation repairable bar fuse
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An Experimental and Analytical Study on Cross-Laminated Bamboo Rocking Walls with Friction Dampers 被引量:2
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作者 Qingfang Lv Tongchen Han +2 位作者 Ye Liu Yi Ding Yujie Lu 《Journal of Renewable Materials》 SCIE EI 2021年第10期1757-1779,共23页
Cross-laminated bamboo(CLB)have a high strength to weight ratio and stable bidirectional mechanical properties.Inspired by the investigation on cross-laminated timber(CLT)rocking walls,CLB rocking walls with conventio... Cross-laminated bamboo(CLB)have a high strength to weight ratio and stable bidirectional mechanical properties.Inspired by the investigation on cross-laminated timber(CLT)rocking walls,CLB rocking walls with conventional friction dampers(CFDs)are studied in this paper.To investigate the mechanical properties of the CLB rocking wall,seven tests are conducted under a cyclic loading scheme,and different test parameters,including the existence of the CFDs,the moment ratio,and the loading times,are discussed.The test results show a bilinear behavior of the CLB rocking wall.The small residual displacements of the CLB rocking wall demonstrate an idealized self-centering capacity.The cumulative energy dissipation curves indicate that the energy dissipation capacity of the CLB rocking wall can be greatly improved with CFDs.The limit states of the CLB rocking wall under a lateral force are proposed based on the strains,stress,and damage level of the CLB material and posttensioned rebar.In addition,an analytical model of the CLB rocking wall is developed based on the proposed limit states of the CLB rocking wall to evaluate the hysteretic response of the CLB rocking wall,and the model is validated by the experimental data.The comparison results show the potential value of the analytical model for engineering design. 展开更多
关键词 Cross-laminated bamboo(CLB) rocking wall friction dampers SELF-CENTERING posttensioned rebar
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Numerical study of a multiple post-tensioned rocking wall-frame system for seismic resilient precast concrete buildings 被引量:1
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作者 Afshin Naserpour Mojtaba Fathi 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2022年第2期377-393,共17页
A multiple rocking wall-frame(MRWF)system,in which the wall panels are directly connected to the adjacent beams and foundation is presented herein.In the MRWF system,the unbonded post-tensioned(PT)tendons are used to ... A multiple rocking wall-frame(MRWF)system,in which the wall panels are directly connected to the adjacent beams and foundation is presented herein.In the MRWF system,the unbonded post-tensioned(PT)tendons are used to promote the self-centering ability,and O-shaped steel dampers are applied to enhance the energy dissipation capacity and reparability of the structure.First,analytical equations are proposed to determine the behavior of the O-shaped dampers.Then,the MRWF system is numerically evaluated for five different models consisting of rocking walls with varying numbers and arrangements while keeping the total effective width of wall panels constant.The numerical results show that with an increase in the number of wall panels and a decrease in the wall width,the hysteretic behavior of the MRWF system tends to the ideal flag-shaped pattern,resulting in little damage to the beams,insignificant strain in the wall toe,negligible residual drifts and damage index of less than 0.2 under severe earthquakes.In contrast,the conventional model demonstrates extensive damage to the structural elements due to undesirable wall-to-frame interaction,which leads to a damage index of 0.78 and residual drifts of 0.42%under seismic loads. 展开更多
关键词 precast wall-frame system rocking behavior POST-TENSIONING O-shaped steel damper residual drift
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Seismic response of rocking isolated railway bridge piers with sacrificial components 被引量:1
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作者 Xia Xiushen Wu Suiwen +3 位作者 Shi Jun Jia Junfeng Chen Xingchong Ma Huajun 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2020年第4期1005-1015,共11页
In this study, sacrificial components were incorporated into self-centering railway bridge piers to improve the lateral stiffness. The seismic response of this new detail was investigated. First, the method to compute... In this study, sacrificial components were incorporated into self-centering railway bridge piers to improve the lateral stiffness. The seismic response of this new detail was investigated. First, the method to compute the initial uplift moment of the self-centering pier is given. In addition, shaking table tests were conducted on a free-rocking pier without sacrificial components, which was used to validate a two-spring numerical model. Good agreement was obtained between the numerical results and experimental data. Furthermore, the validated model was employed to investigate the influence of sacrificial components on the seismic response of rocking piers. For this purpose, two models were developed, with and without sacrificial components. Nonlinear response history analysis was then performed on both models under three historical motions. The results showed that compared to the one without sacrificial components, the rocking pier with sacrificial components has comparable displacement at the top of the pier, and maximum uplift moment at high amplitude motion. Therefore, incorporating sacrificial components into the rocking pier can increase the lateral stiffness at service load and low amplitude frequent earthquakes but can produce comparable response at high seismic excitation. These results provide support for performance-based seismic design of self-centering rocking piers. 展开更多
关键词 self-centering rocking pier shake table tests sacrificial components nonlinear response history analysis
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Optimization of design parameters for controlled rocking steel braced dual-frames
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作者 Sobhan Ghasemi M.Firoozi Nezamabadi +1 位作者 Abdolreza S.Moghadam Mahmood Hosseini 《Earthquake Engineering and Engineering Vibration》 SCIE EI CSCD 2022年第4期1053-1068,共16页
A controlled rocking concentrically steel braced frame(CR-CSBF)is introduced as an alternative to conventional methods to prevent major structural damage during large earthquakes.It is equipped with elastic post-tensi... A controlled rocking concentrically steel braced frame(CR-CSBF)is introduced as an alternative to conventional methods to prevent major structural damage during large earthquakes.It is equipped with elastic post-tensioned(PT)cables and replaceable devices or fuses to provide overturning resistance and dissipate energy,respectively.Although CR-CSBFs are not officially legalized in globally valid codes for new buildings,it is expected to be presented in them in the near future.The main goal of this study is to determine the optimal design parameters consist of the yield strength and modulus of elasticity of the fuse,the initial force of the PT cable,and the gravity load on the rocking column,considering different heights of the frame,spanning ratios and ground motion types for dual-configuration CR-CSBF.Nonlinear time-history analyses are performed in OpenSees.This study aims to define the optimal input variables as effective design parameters of CR-CSBFs by comparing four seismic responses consisting of story drift,roof displacement,roof acceleration and base shear,and also using the Euclidean metric optimization method.Despite the previous research,this study is innovative and first of its kind.The results demonstrate that the optimal design parameters are variable for various conditions. 展开更多
关键词 self-centering rocking steel braced frame design parameters OPTIMIZATION low damage
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Rocking Compliance of Rigid Rectangular Foundations on an Elastic Half-space
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作者 吴知丰 《Journal of Harbin Institute of Technology(New Series)》 EI CAS 1995年第2期64-68,共5页
RockingComplianceofRigidRectangularFoundationsonanElasticHalf-spaceWuZhifeng(吴知丰)(Dept.ofCivilEngineering,Ha... RockingComplianceofRigidRectangularFoundationsonanElasticHalf-spaceWuZhifeng(吴知丰)(Dept.ofCivilEngineering,HarbinInstituteofTe... 展开更多
关键词 ss:Rigid FOUNDATION rocking COMPLIANCE soil-foundation
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Development of rocking constraint device with vertical damping capacity for three-dimensional base-isolated frame structures
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作者 Yundong SHI Qi WANG +1 位作者 Wenqing DONG Bo ZHAO 《Frontiers of Structural and Civil Engineering》 SCIE EI CSCD 2023年第3期350-367,共18页
A new rocking constraint device(RCD)is developed for three-dimensional(3D)base-isolated frame structures by connecting a custom-designed cylinder pair to provide vertical damping with replaceable damping components in... A new rocking constraint device(RCD)is developed for three-dimensional(3D)base-isolated frame structures by connecting a custom-designed cylinder pair to provide vertical damping with replaceable damping components installed outside the cylinders when the superstructure undergoes translational motion,and rocking constraint capacity when the superstructure is susceptible to rocking.Theoretical formulas for calculating the damping and rocking constraint stiffness of the RCD are proposed.Two series of sinusoidal loading tests are conducted at different loading frequencies and amplitudes to verify the damping and rocking constraint performance of the RCD.The test results show that the cylinder without orifices on its piston can provide the desired damping with a replaceable damping component,and that the RCD can effectively suppress rocking.Although the vertical stiffness of an individual cylinder is affected by the location of the replaceable damping component and loading frequency,the average vertical stiffness of the two cylinders,which determines the rocking constraint stiffness of the RCD,is independent of the two factors.Comparisons of the test and theoretical results indicate that the errors of the proposed formulas for calculating the damping and rocking constraint stiffness of the RCD do not exceed 12.9%and 11.0%,respectively. 展开更多
关键词 three-dimensional isolation rocking behavior rocking constraint device replaceable damping component sinusoidal test
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Research progress of“rocking chair”type zinc-ion batteries with zinc metal-free anodes
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作者 Le Li Shaofeng Jia +3 位作者 Minghui Cao Yongqiang Ji Hengwei Qiu Dan Zhang 《Chinese Chemical Letters》 SCIE CAS CSCD 2023年第12期42-52,共11页
"Rocking chair"type lithium-ion batteries with lithium metal-free anodes have been successfully com-mercialized over the past few decades.Zinc-ion batteries(zIBs)have gained increasing attention in recent ye... "Rocking chair"type lithium-ion batteries with lithium metal-free anodes have been successfully com-mercialized over the past few decades.Zinc-ion batteries(zIBs)have gained increasing attention in recent years given their safety,greenness,ease of manufacture,and cost-efficiency.Nevertheless,the practical application of ZIBs is largely hindered by the dendritic growth of the Zn metal anode,low Coulombic eficiency,great harm,and existence of various side reactions.Herein,this review provides a systematic overview of emerging"rocking chair"type ZIBs with zinc metal-free anodes.Firstly,the basic fundamen-tals,advantages,and challenges of“rocking chair”type ZIBs are introduced.Subsequently,an overview of the design principles and recent progress of"rocking chair"type ZIBs with zinc metal-free anodes are presented.Finally,the key challenges and perspectives for future advancement of"rocking chair"type ZiBs with zinc metal-free anodes are proposed.This review is anticipated to attracted increased focus to metal-free anodes"rocking chair"type metal-ion battery and provide new inspirations for the develop-ment of high-energy metal-ion batteries. 展开更多
关键词 Zinc ion batteries(ZIBs) "rocking chair"type Zinc metal-free anode Transition metal dichalcogenides Transition metal oxides Organic compounds
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Rocking Out!
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作者 Wei Yao(Text/photos) 《Beijing Review》 2023年第13期46-47,共2页
A rock concert for kids took to the stage of a theater nestled in a hutong,a narrow alleyway in the traditional residential areas of Beijing,on March 18.Chinese and British performers put on a musical extravaganza tha... A rock concert for kids took to the stage of a theater nestled in a hutong,a narrow alleyway in the traditional residential areas of Beijing,on March 18.Chinese and British performers put on a musical extravaganza that had children stomping their feet,shaking their heads and rocking out to the music. 展开更多
关键词 SHAKING ROCK NARROW
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SLC9A3-AS1调控miR-148a-3p/ROCK1信号轴影响肾癌细胞生物学功能
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作者 向威 吕磊 +2 位作者 郑福鑫 章传华 袁敬东 《华中科技大学学报(医学版)》 CAS CSCD 北大核心 2024年第2期161-167,共7页
目的检测lncRNA SLC9A3-AS1在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)组织与肾癌细胞中的表达水平,探讨其促进肾癌细胞恶性生物学行为的机制。方法应用GEPIA2在线软件(http://gepia2.cancer-pku.cn/)分析TCGA数据库中SLC9... 目的检测lncRNA SLC9A3-AS1在肾透明细胞癌(clear cell renal cell carcinoma,ccRCC)组织与肾癌细胞中的表达水平,探讨其促进肾癌细胞恶性生物学行为的机制。方法应用GEPIA2在线软件(http://gepia2.cancer-pku.cn/)分析TCGA数据库中SLC9A3-AS1在ccRCC组织中的表达水平;采用实时荧光定量聚合酶链反应(qRT-PCR)检测SLC9A3-AS1在不同肾癌细胞系、24例ccRCC组织与癌旁正常肾脏组织中的表达水平;应用细胞增殖/毒性检测试剂盒(cell counting kit-8,CCK-8)和Transwell小室迁移实验检测敲低SLC9A3-AS1表达对肾癌细胞增殖与迁移的影响;应用蛋白质免疫印迹法检测增殖与迁移相关信号通路蛋白的表达水平;采用双荧光素酶报告基因实验验证SLC9A3-AS1与miR-148a-3p/ROCK1轴的靶向调控关系。结果GEPIA2软件分析结果显示,相较正常肾脏组织,SLC9A3-AS1在肾透明细胞癌组织中表达显著上调(P<0.01)。qRT-PCR结果显示,相较癌旁正常肾脏组织,SLC9A3-AS1在24例ccRCC组织中表达显著上调(P<0.01);相较永生化肾小管上皮细胞,SLC9A3-AS1在4种肾癌细胞系中表达均显著上调,以786-O细胞最为显著(P<0.01)。干扰SLC9A3-AS1表达,可显著抑制786-O细胞的增殖与迁移能力,上调E-cadherin的蛋白表达水平,而下调N-cadherin、MMP2的蛋白表达水平(均P<0.05);过表达miR-148a-3p可显著抑制786-O细胞的增殖与迁移能力(P<0.01)。双荧光素酶报告基因检测结果表明,SLC9A3-AS1可特异性结合miR-148a-3p,后者进一步靶向结合ROCK1 mRNA的3′UTR区域;过表达miR-148a-3p可明显下调ROCK1 mRNA的表达水平,敲低miR-148a-3p表达则产生相反的效应;敲低SLC9A3-AS1表达可显著下调786-O细胞中ROCK1 mRNA与蛋白的表达水平(P<0.01);下调miR-148a-3p表达可部分逆转SLC9A3-AS1沉默对786-O细胞增殖与迁移的抑制作用(P<0.05)。结论SLC9A3-AS1在ccRCC中通过调控miR-148a-3p/ROCK1轴发挥促癌作用,有望成为ccRCC的一个新的分子标志物。 展开更多
关键词 肾透明细胞癌 SLC9A3-AS1 miR-148a-3p ROCK1
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桃叶珊瑚苷调节RhoA/ROCK信号通路对胶质母细胞瘤细胞活力和上皮间质转化的影响
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作者 李娟 石海平 李维民 《天津中医药》 CAS 2024年第3期386-392,共7页
[目的]研究桃叶珊瑚苷(AU)对胶质母细胞瘤(GBM)细胞活力和上皮间质转化(EMT)的影响,并对其作用机制进行探讨。[方法]将U87细胞随机分为对照组、AU低浓度组、AU中浓度组、AU高浓度组、Y-27632组、AU高浓度+Y-27632组。细胞计数器试剂盒(C... [目的]研究桃叶珊瑚苷(AU)对胶质母细胞瘤(GBM)细胞活力和上皮间质转化(EMT)的影响,并对其作用机制进行探讨。[方法]将U87细胞随机分为对照组、AU低浓度组、AU中浓度组、AU高浓度组、Y-27632组、AU高浓度+Y-27632组。细胞计数器试剂盒(CCK-8)法检测细胞活力,流式细胞术检测细胞凋亡,Transwell小室实验检测细胞迁移和侵袭,蛋白免疫印迹法(Western Blot)检测基质金属蛋白酶(MMP)2、MMP9、波形蛋白(Vimentin)、上皮钙黏蛋白(E-cadherin)、神经钙黏蛋白(N-cadherin)、Ras同源基因家族成员A(RhoA)、Rho相关卷曲螺旋蛋白激酶(ROCK)1、ROCK2表达。构建GBM裸鼠模型,随机分为裸鼠对照组、AU组、Y-27632组、AU+Y-27632组,测量肿瘤质量与体积,免疫组化法检测移植瘤组织RhoA、ROCK1、ROCK2蛋白表达。[结果]GBM细胞活力随着AU浓度的升高而逐渐降低(P<0.05),选择U87作为后续实验细胞,选择10、25、50μmol/L浓度作为AU后续实验浓度。与对照组比较,AU低、中、高浓度组和Y-27632组细胞活力、迁移和侵袭细胞数、MMP2、MMP9、N-cadherin、Vimentin、RhoA、ROCK1、ROCK2表达显著下降,凋亡率、E-cadherin表达显著升高(P<0.05),其中高浓度AU和Y-27632组共同处理的细胞变化更显著(P<0.05)。AU和Y-27632均能抑制移植瘤质量和体积,降低RhoA/ROCK信号通路蛋白表达(P<0.05)。[结论]AU能抑制GBM细胞活力、迁移侵袭和EMT,促进细胞凋亡,其作用机制可能与抑制RhoA/ROCK信号通路有关。 展开更多
关键词 胶质母细胞瘤 细胞活力 上皮间质转化 桃叶珊瑚苷 RhoA/ROCK信号通路
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桂楼复方对子宫腺肌病异位内膜细胞迁移和侵袭的影响研究
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作者 张一诺 王子璐 +6 位作者 石雅馨 王信 高新宇 张全英 喻梦蝶 徐丽 师伟 《中国全科医学》 CAS 北大核心 2024年第24期3007-3014,共8页
背景子宫腺肌病(AM)是妇科常见的疑难病,但其具体机制尚未明确。中医药治疗AM有一定优势,前期研究显示,桂楼复方宫内缓释系统可明显降低AM模型大鼠子宫内膜细胞增殖、侵袭能力。目的探讨桂楼复方对子宫腺肌病异位内膜细胞(AMDC)迁移、... 背景子宫腺肌病(AM)是妇科常见的疑难病,但其具体机制尚未明确。中医药治疗AM有一定优势,前期研究显示,桂楼复方宫内缓释系统可明显降低AM模型大鼠子宫内膜细胞增殖、侵袭能力。目的探讨桂楼复方对子宫腺肌病异位内膜细胞(AMDC)迁移、侵袭的影响,并研究其对Rho/ROCK信号通路的影响。方法本研究于2021年10月—2023年4月在山东中医药大学附属医院实验中心完成。使用CCK-8法检测AMDC细胞活力并筛选最佳药物浓度。设置空白组,使用桂楼复方(50、100 mg/L)处理AMDC,划痕实验检测细胞迁移能力,Transwell实验检测细胞侵袭能力,免疫印迹法Western Blotting)/实时荧光定量PCR(RT-qPCR)法检测RhoA、RhoB、RhoC、ROCK1、ROCK2在蛋白和mRNA水平的表达情况。Rescue实验中使用激动剂U-46619(1μmol/L)和桂楼复方(100 mg/L)处理AMDC,Western Blotting法检测RhoA、RhoB、RhoC、ROCK1、ROCK2的蛋白表达改变情况。结果与空白组比较,100 mg/L桂楼复方干预后AMDC细胞存活率未明显降低,差异无统计学意义(P>0.05),是不影响细胞活性的最佳药物浓度。选取50、100 mg/L桂楼复方进行后续实验。与空白组比较,桂楼复方50 mg/L、100 mg/L组AMDC迁移、侵袭能力受到抑制(P<0.001),RhoA、RhoC、ROCK1、ROCK2的蛋白及mRNA表达水平下调(P<0.001),U-466191μmol/L组RhoA、RhoC、ROCK1、ROCK2的蛋白表达上调(P<0.001)。与U-466191μmol/L组比较,U-466191μmol/L联合桂楼复方100 mg/L组RhoA、RhoC、ROCK1、ROCK2的蛋白表达下调(P<0.001)。空白组与桂楼复方50 mg/L、100 mg/L组RhoB的蛋白及mRNA表达水平差异均无统计学意义(P>0.05)。结论桂楼复方可有效抑制AMDC的迁移、侵袭能力,不影响细胞活性的最佳药物浓度为100 mg/L,桂楼复方可下调RhoA、RhoC、ROCK1、ROCK2的蛋白及mRNA表达水平,并可逆转U-46619对RhoA、RhoC、ROCK1、ROCK2蛋白表达水平的上调作用,从而抑制AM病局部异位病灶的持续进展,其作用可能与调控Rho/ROCK通路密切相关。 展开更多
关键词 子宫腺肌病 桂楼复方 子宫内膜 子宫腺肌病异位内膜细胞 迁移 侵袭 RHO/ROCK信号通路
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艾灸联合保真汤加减治疗糖尿病肾病的疗效观察及对CT灌注参数、Rho/ROCK信号通路蛋白的影响
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作者 赵小艳 王俊杰 《上海针灸杂志》 CSCD 2024年第1期17-23,共7页
目的 观察艾灸联合保真汤加减治疗糖尿病肾病气阴两虚证的临床疗效及对CT灌注参数、Rho/ROCK信号通路蛋白的影响。方法 将146例糖尿病肾病气阴两虚证患者随机分为观察组(74例)和对照组(72例)。在对症治疗的基础上,对照组给予厄贝沙坦片... 目的 观察艾灸联合保真汤加减治疗糖尿病肾病气阴两虚证的临床疗效及对CT灌注参数、Rho/ROCK信号通路蛋白的影响。方法 将146例糖尿病肾病气阴两虚证患者随机分为观察组(74例)和对照组(72例)。在对症治疗的基础上,对照组给予厄贝沙坦片,观察组给予艾灸联合保真汤加减。观察两组中医主症积分、CT灌注参数[血尿素氮(blood urea nitrogen, BUN)、血肌酐(serum creatinine, SCr)、尿白蛋白排泄率(urinary albumin excretion rate, UAER)和24 h尿蛋白定量(24-hour urinary protein quantification, 24 h Upro)]、肾血流指标[舒张末期血流速度(end-diastolic velocity, EDV)、肾段动脉的收缩期峰值流速(peak-systolic velocity, PSV)、搏动指数(pulsatility index, PI)和阻力指数(resistive index, RI)]、血清炎性因子[肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)、白细胞介素-1β(interleukin-1β, IL-1β)、白细胞介素-6(interleukin-6, IL-6)和C反应蛋白(C-reactive protein, CRP)]水平及Rho/ROCK信号通路[Ras同源基因家族成员A(Ras homolog gene family member A, RhoA)、Rho关联含卷曲螺旋蛋白激酶Ⅰ(Rho-associated coiled-coil containing protein kinaseⅠ, ROCKI)、α-平滑肌肌动蛋白(α-smooth muscle actin, α-SMA)和钙黏附蛋白-E(E-cadherin, E-Cad)]蛋白水平,并比较两组临床疗效及不良反应。结果 观察组总有效率为97.3%(72/74),明显高于对照组的81.9%(59/72)(P<0.05)。观察组治疗后中医主症积分低于治疗前和对照组(P<0.05)。两组治疗后CT灌注参数降低(P<0.05),且观察组低于对照组(P<0.05)。观察组治疗后PSV、EDV较治疗前和对照组加快(P<0.05),RI、PI较治疗前和对照组降低(P<0.05)。观察组治疗后血清炎性因子水平较治疗前和对照组降低(P<0.05)。观察组治疗后Rho A、ROCKI、α-SMA蛋白水平较治疗前和对照组降低(P<0.05),E-Cad蛋白水平较治疗前和对照组升高(P<0.05)。观察组不良反应发生率为1.4%(1/74),低于对照组的19.4%(14/72)(P<0.05)。结论 在对症治疗的基础上,艾灸联合保真汤加减可明显提高糖尿病肾病气阴两虚证患者的治疗效果,其机制可能与改善CT灌注参数,调节血清Rho/ROCK信号通路蛋白相关。 展开更多
关键词 灸法 糖尿病并发症 糖尿病肾病 气阴两虚 Rho/ROCK信号通路蛋白
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