Root border cells （RBCs） originate from the root tip epidermis and surround the root apices. In this study, we evaluated the developmental characteristics and the roles of RBCs in protection of root apices of cucumb...Root border cells （RBCs） originate from the root tip epidermis and surround the root apices. In this study, we evaluated the developmental characteristics and the roles of RBCs in protection of root apices of cucumber and ifgleaf gourd seedlings from CA toxicity. The formation of RBCs and the emergence of the root tip occurred almost simultaneously in root apices of cucumber and ifgleaf gourd seedlings. CA ranging from 0 to 0.25 mol L-1 inhibited root elongation and decreased root cell viability in the root tip, moreover the inhibitory effects of CA were more signiifcant in the CA-sensitive cucumber than in the CA-tolerant ifgleaf gourd. Removal of RBCs from root tips led to more severe CA induced inhibition of root elongation and decline in root cell viability. Increasing CA levels and treatment time decreased the relative viability of attached and detached RBCs. CA also induced a thicker mucilage layer surrounding attached RBCs of both species. Additionally, a signiifcantly higher relative cell viability of attached RBCs and thicker mucilage layers were observed in ifgleaf gourd. These results suggest that RBCs play an important role in protecting root tips from CA toxicity.展开更多
The extract of crofton weed(Eupatorium adenophorum) inhibits seed germination and weed growth;however,the physiological mechanisms underlying the effect of crofton weed extract on the modulation of seedling growth and...The extract of crofton weed(Eupatorium adenophorum) inhibits seed germination and weed growth;however,the physiological mechanisms underlying the effect of crofton weed extract on the modulation of seedling growth and root system development remain largely unclear.In this study,we investigated the effects of the leaf extract of crofton weed(LECW) on primary root(PR) growth in maize seedlings.Treatment with LECW markedly inhibited seed germination and seedling growth in a dose-dependent manner.Physiological analysis indicated that the LECW induced reactive oxygen species(ROS) accumulation in root tips,thereby leading to cell swelling and deformation both in the root cap and elongation zone of root tips,finally leading to cell death in root border cells(RBCs) and PR growth inhibition.The LECW also inhibited pectin methyl esterase(PME) activity,thereby decreasing the RBC number.Taken together,our results indicated that the LECW inhibited PR growth by inducing ROS accumulation and subsequent cell death in RBCs.The present study provides a better understanding of how the LECW modifies root system development and provides insight for evaluating the toxicity of crofton weed extracts in plants.展开更多
基金supported by the National Basic Research Program of China(973 Program2009CB1190002)the National Key Technology R&D Program of China(2011BAD12B01)
文摘Root border cells （RBCs） originate from the root tip epidermis and surround the root apices. In this study, we evaluated the developmental characteristics and the roles of RBCs in protection of root apices of cucumber and ifgleaf gourd seedlings from CA toxicity. The formation of RBCs and the emergence of the root tip occurred almost simultaneously in root apices of cucumber and ifgleaf gourd seedlings. CA ranging from 0 to 0.25 mol L-1 inhibited root elongation and decreased root cell viability in the root tip, moreover the inhibitory effects of CA were more signiifcant in the CA-sensitive cucumber than in the CA-tolerant ifgleaf gourd. Removal of RBCs from root tips led to more severe CA induced inhibition of root elongation and decline in root cell viability. Increasing CA levels and treatment time decreased the relative viability of attached and detached RBCs. CA also induced a thicker mucilage layer surrounding attached RBCs of both species. Additionally, a signiifcantly higher relative cell viability of attached RBCs and thicker mucilage layers were observed in ifgleaf gourd. These results suggest that RBCs play an important role in protecting root tips from CA toxicity.
基金the Key Project of Science and Technology of Shanxi Province(20150311016-5)the Science and Technology innovation Foundation of Shanxi Agricultural University(2017ZZ09)。
文摘The extract of crofton weed(Eupatorium adenophorum) inhibits seed germination and weed growth;however,the physiological mechanisms underlying the effect of crofton weed extract on the modulation of seedling growth and root system development remain largely unclear.In this study,we investigated the effects of the leaf extract of crofton weed(LECW) on primary root(PR) growth in maize seedlings.Treatment with LECW markedly inhibited seed germination and seedling growth in a dose-dependent manner.Physiological analysis indicated that the LECW induced reactive oxygen species(ROS) accumulation in root tips,thereby leading to cell swelling and deformation both in the root cap and elongation zone of root tips,finally leading to cell death in root border cells(RBCs) and PR growth inhibition.The LECW also inhibited pectin methyl esterase(PME) activity,thereby decreasing the RBC number.Taken together,our results indicated that the LECW inhibited PR growth by inducing ROS accumulation and subsequent cell death in RBCs.The present study provides a better understanding of how the LECW modifies root system development and provides insight for evaluating the toxicity of crofton weed extracts in plants.
