叶绿体rps16基因作为叶绿体重要基因,广泛应用于植物系统发育研究,而甘蓝型油菜rps16基因多态性研究尚未见报道。本研究利用二维Eco TILLING(ecotype targeting induced local lesions in genomes)技术及Sanger测序技术对全球256份甘蓝...叶绿体rps16基因作为叶绿体重要基因,广泛应用于植物系统发育研究,而甘蓝型油菜rps16基因多态性研究尚未见报道。本研究利用二维Eco TILLING(ecotype targeting induced local lesions in genomes)技术及Sanger测序技术对全球256份甘蓝型油菜种质资源的rps16基因进行多态性分析。结果发现,256份甘蓝型油菜rps16基因有5个多态性位点,经测序检测其存在4种单倍型;5个SNP位点都位于非编码序列上。研究结果表明,rps16基因在不同材料中具有丰富的遗传变异,可为其它基因遗传多样性研究提供借鉴,有助于对油菜种质资源进行评估和多样性分类。展开更多
Exploratory surveys were carried out in the Andamans and Nicobar group of islands during 2006 and 2014 to locate wild species viz. Mangifera andamanica King, Mangifera camptosperma Pierre and Mangifera griffithii Hook...Exploratory surveys were carried out in the Andamans and Nicobar group of islands during 2006 and 2014 to locate wild species viz. Mangifera andamanica King, Mangifera camptosperma Pierre and Mangifera griffithii Hook. Not much variation was observed for fruit shape and size for the species Mangifera andamanica, which was endemic to this region. The species M. griffithii has been reported to be only in Mt. Harriet. However, another plant of M. griffithii in the Shoalbay region was found during the second survey. The foliage & fruit characteristics of the two specimens were similar, with a slight difference in the morphological features, which could be attributed to their origin from seeds. The DNA finger printing carried out showed minor changes in the species. The phylogenetic relationships amongst five Mangifera species viz. M. indica, M. griffithii, M. camptosperma, M. odorata and M. andamanica were analyzed by employing chloroplast markers viz., petB-petD intergenic spacer, rps16 gene, trnL-trnF intergenic spacer and nuclear marker—External Transcribed Spacer (ETS). The nuclear markers and chloroplast markers based on phylogenetic analysis showed that the common mango M. indica L. was closely related to M. griffithii and M. camptosperma, which belonged to subgenus Mangifera. However, M. odorata that belonged to subgenus Limus was grouped separately along with M. andamanica. The above results are in congruent with the accepted classification of genus Mangifera reported by Kostermans and Bompard with the exception of M. andamanica, which has been earlier classified under subgenus Mangifera. Results clearly indicated that classification of M. andamanica under subgenus needed to be reconsidered.展开更多
文摘叶绿体rps16基因作为叶绿体重要基因,广泛应用于植物系统发育研究,而甘蓝型油菜rps16基因多态性研究尚未见报道。本研究利用二维Eco TILLING(ecotype targeting induced local lesions in genomes)技术及Sanger测序技术对全球256份甘蓝型油菜种质资源的rps16基因进行多态性分析。结果发现,256份甘蓝型油菜rps16基因有5个多态性位点,经测序检测其存在4种单倍型;5个SNP位点都位于非编码序列上。研究结果表明,rps16基因在不同材料中具有丰富的遗传变异,可为其它基因遗传多样性研究提供借鉴,有助于对油菜种质资源进行评估和多样性分类。
文摘Exploratory surveys were carried out in the Andamans and Nicobar group of islands during 2006 and 2014 to locate wild species viz. Mangifera andamanica King, Mangifera camptosperma Pierre and Mangifera griffithii Hook. Not much variation was observed for fruit shape and size for the species Mangifera andamanica, which was endemic to this region. The species M. griffithii has been reported to be only in Mt. Harriet. However, another plant of M. griffithii in the Shoalbay region was found during the second survey. The foliage & fruit characteristics of the two specimens were similar, with a slight difference in the morphological features, which could be attributed to their origin from seeds. The DNA finger printing carried out showed minor changes in the species. The phylogenetic relationships amongst five Mangifera species viz. M. indica, M. griffithii, M. camptosperma, M. odorata and M. andamanica were analyzed by employing chloroplast markers viz., petB-petD intergenic spacer, rps16 gene, trnL-trnF intergenic spacer and nuclear marker—External Transcribed Spacer (ETS). The nuclear markers and chloroplast markers based on phylogenetic analysis showed that the common mango M. indica L. was closely related to M. griffithii and M. camptosperma, which belonged to subgenus Mangifera. However, M. odorata that belonged to subgenus Limus was grouped separately along with M. andamanica. The above results are in congruent with the accepted classification of genus Mangifera reported by Kostermans and Bompard with the exception of M. andamanica, which has been earlier classified under subgenus Mangifera. Results clearly indicated that classification of M. andamanica under subgenus needed to be reconsidered.
