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The gut microbiome and metabolome of Himalayan Griffons(Gyps himalayensis):insights into the adaptation to carrion-feeding habits in avian scavengers 被引量:1
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作者 Wen Wang Xiaolong Gao +9 位作者 Sisi Zheng Zhuoma Lancuo Ying Li Lilin Zhu Jianping Hou Jiayi Hai Xin Long Hanxi Chen Alexey Druzyaka Kirill Sharshov 《Avian Research》 SCIE CSCD 2021年第4期682-698,共17页
Background:Himalayan Griffons(Gyps himalayensis),large scavenging raptors widely distributed in Qinghai-Tibetan Plateau,have evolved a remarkable ability to feed on carcasses without suffering any adverse effects.The ... Background:Himalayan Griffons(Gyps himalayensis),large scavenging raptors widely distributed in Qinghai-Tibetan Plateau,have evolved a remarkable ability to feed on carcasses without suffering any adverse effects.The gut microbiome plays an important role in animal physiological and pathological processes,and has also been found to play a health protective role in the vulture adaptation to scavenging.However,the microbial taxonomic diversity(including nonculturable and culturable microbes),functions,and metabolites related to Himalayan Griffons have not been fully explored.Methods:In the present study,the 28 fecal samples of the Himalayan Griffons and 8 carrion samples were collected and sequenced using high-throughput 16 S rRNA gene sequencing methods to analyze the composition and functional structures of the microbiomes.Twelve fecal samples of the Himalayan Griffons were analyzed using untargeted Liquid Chromatography Mass Spectroscopy(LC-MS)to identify metabolites.We used different culture conditions to grow Himalayan Griffons gut microbes.Inhibitory effects of gut beneficial bacteria on 5 common pathogenic bacteria were also tested using the Oxford cup method.Results:According to the results of the culture-independent method,a high abundance of four major phyla in Himalayan Griffons were identified,including Fusobacteria,Firmicutes,Bacteroidetes,and Proteobacteria.The most abundant genera were Fusobacterium,followed by Clostridium_sensu_stricto_1,Cetobacterium,Epulopiscium,and Bacteroides.The predicted primary functional categories of the Himalayan Griffons'gut microbiome were associated with carbohydrate and amino acid metabolism,replication and repair,and membrane transport.LC-MS metabolomic analysis showed a total of 154 metabolites in all the fecal samples.Cultivation yielded 184 bacterial isolates with Escherichia coli,Enterococcus faecium,Enterococcus hirae,and Paeniclostridium sordellii as most common isolates.Moreover,7 potential beneficial gut bacteria isolated showed certain inhibition to 5 common pathogenic bacteria.Conclusions:Our findings broaden and deepen the understanding of Himalayan Griffons'gut microbiome,and highlighted the importance of gut microbiome-mediated adaptation to scavenging habits.In particular,our results highlighted the protective role of gut beneficial bacteria in the Himalayan Griffons against pathogenic bacteria that appear in rotten food resources. 展开更多
关键词 Avian scavengers Culture MICROBIOME Pathogens VULTURES
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Assessment of the Activities of Scavengers in Obio/Akpor Local Government, Rivers State, Nigeria
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作者 Elenwo Ephraim Ikechukwu 《Journal of Environmental Protection》 2015年第3期272-280,共9页
This study was carried out based on consistent observations on the activities of scavengers of scrap metal in Obio/Akpor local government Rivers State. The study was carried out to ascertain the profitability of scrap... This study was carried out based on consistent observations on the activities of scavengers of scrap metal in Obio/Akpor local government Rivers State. The study was carried out to ascertain the profitability of scrap metal scavenging and how scrap metal waste can generate wealth to the public and the government. The study made use of primary and secondary data with the former being the main focus. The data were analyzed using the simple percentage, while the hypothesis was tested by means of the chi-square. The study findings revealed that there was a relationship between waste and wealth in relation to scrap metal scavenging. The researches therefore make the following recommendations;that scavengers should form co-operatives so that they can pull their efforts and resources together and bargain collectively. Government and nongovernmental organization should enlighten them through seminars, workshops, etc., on how to carry out their activities in an environmentally safe manner. The state government should encourage scavenging by enacting a law to back the profession and make it attractive for the teaming youths to venture into. 展开更多
关键词 ASSESSMENT Activities scavengers Obio/Akpor
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Effects of a nitric oxide donor and nitric oxide scavengers on Sorbus pohuashanensis embryo germination 被引量:2
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作者 Ling Yang Dongyan Zhang +3 位作者 Hongnan Liu Cheng Wei Jianan Wang Hailong Shen 《Journal of Forestry Research》 SCIE CAS CSCD 2018年第3期628-635,共8页
The effects of an exogenous nitric oxide donor(sodium nitroprusside, SNP), a NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxode(PTIO) and carboxy-PTIO potassium salt(c PTIO) on the embryo germination ... The effects of an exogenous nitric oxide donor(sodium nitroprusside, SNP), a NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxode(PTIO) and carboxy-PTIO potassium salt(c PTIO) on the embryo germination of Sorbus pohuashanensis were studied in a petri dish test. SNP at 0.5–5 mmol L-1 increased germination percentage, mean time to germination, germination index and germination energy compared with the control to different degrees. Treatment with 2 mmol L-1 SNP improved germination most significantly; embryo germination percentage for mother tree 1(91.11%) and mother tree 2(64.44%) were much higher than the control. In addition,excessive SNP levels did not enhance embryo germination.Combined treatment with SNP and an NO scavenger delayed embryo germination. Treatment with c PTIO inhibited embryo germination; germination percentage was 42.22% and was lower than that of the control. These results show that low concentrations of exogenous NO can enhance the embryo germination of S. pohuashanensis,providing a simple, effective way for promoting germination of S. pohuashanensis. 展开更多
关键词 Mountain ash EMBRYO germination NITRIC OXIDE Sodium NITROPRUSSIDE NITRIC OXIDE SCAVENGER
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Indirect photocatalytic reduction of arsenate to arsenite in aqueous solution with TiO_2 in the presence of hole scavengers
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作者 Abdus Samad Shamim Ahsan +4 位作者 Ikki Tateishi Mai Furukawa Hideyuki Katsumata Tohru Suzuki Satoshi Kaneco 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2018年第3期529-533,共5页
The indirect photocatalytic reduction of arsenate to arsenite in aqueous solution with titanium dioxide(TiO_2)was investigated with various hole scavengers such as methanol, ethanol, 2-propanol, formaldehyde, acetone,... The indirect photocatalytic reduction of arsenate to arsenite in aqueous solution with titanium dioxide(TiO_2)was investigated with various hole scavengers such as methanol, ethanol, 2-propanol, formaldehyde, acetone,formic acid and acetic acid. Although the direct photocatalytic reduction of arsenate to arsenite with TiO_2 was impossible, an indirect reduction of As(V) was possible in the presence of sacrificial electron donors to form strongly reductive radicals. The addition of ethanol was very effective for indirect photocatalytic reduction of As(V) in aqueous solution with TiO_2 photocatalyst. The indirect photocatalytic reduction rate of As(V) may be related with both the reaction rate constants of reaction of hydroxyl radicals with hole scavenger and the reactivities for the radicals M· which are produced by the reaction of ·OH with hole scavenger. 展开更多
关键词 亚砷酸盐 TiO2 间接 反应率 二氧化物 光催化剂 分子
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自发性急性脑出血患者血浆sCD163/sTWEAK比值与预后的关系
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作者 张文超 杨雪辉 +2 位作者 尹涛 王睿健 张盟盟 《天津医药》 CAS 2024年第3期297-301,共5页
目的探究自发性急性脑出血(ACH)患者血浆可溶性CD163(sCD163)/可溶性肿瘤坏死因子样凋亡弱诱导因子(sTWEAK)比值与预后的关系。方法纳入ACH患者90例作为病例组,根据格拉斯哥预后评分将病例组分为预后不良组(38例)和预后良好组(52例);另... 目的探究自发性急性脑出血(ACH)患者血浆可溶性CD163(sCD163)/可溶性肿瘤坏死因子样凋亡弱诱导因子(sTWEAK)比值与预后的关系。方法纳入ACH患者90例作为病例组,根据格拉斯哥预后评分将病例组分为预后不良组(38例)和预后良好组(52例);另选取同期体检健康者45例为对照组。酶联免疫吸附试验检测血浆sCD163、sTWEAK水平并计算sCD163/sTWEAK比值。分析血浆sCD163、sTWEAK水平及sCD163/sTWEAK比值与临床资料的相关性;Logistic回归分析ACH患者预后不良的影响因素;受试者工作特征(ROC)曲线分析sCD163/sTWEAK比值对ACH患者预后不良的预测价值。结果病例组血浆sCD163、sTWEAK水平及sCD163/sTWEAK比值均显著高于对照组;预后良好组上述指标均低于预后不良组(P<0.05)。预后良好组血肿体积、美国国立卫生研究院卒中量表(NIHSS)评分、高血压及幕下出血比例均低于预后不良组,低密度脂蛋白胆固醇(LDL-C)高于预后不良组(P<0.05)。相关性分析表明,血浆sCD163、sTWEAK水平及sCD163/sTWEAK比值与出血部位、血肿体积、NIHSS评分、白细胞计数、血小板计数、中性粒细胞/淋巴细胞比值(NLR)呈正相关(P<0.05)。Logistic回归分析显示,sCD163/sTWEAK比值、出血部位、血肿体积、NIHSS评分为ACH患者预后不良的影响因素(P<0.05)。ROC曲线结果表明,sCD163/sTWEAK比值评估ACH患者预后不良的AUC为0.850,敏感度和特异度分别为86.84%和69.23%。结论sCD163/sTWEAK比值在ACH患者血浆中水平较高,并与预后不良有关,该值对此类患者的预后有一定预测价值。 展开更多
关键词 脑出血 细胞因子TWEAK 预后 可溶性血红蛋白清道夫受体163蛋白 可溶性肿瘤坏死因子样凋亡弱诱导因子
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Can scavengers save zebras from anthrax? A modeling study 被引量:1
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作者 Crystal Mackey Christopher Kribs 《Infectious Disease Modelling》 2021年第1期56-74,共19页
Namibia’s Etosha National Park(ENP)is home to many different animals such as lions,jackals,hyenas,zebras,elephants,etc.Each year,grazing animals are infected and die from anthrax caused by the bacteria Bacillus anthr... Namibia’s Etosha National Park(ENP)is home to many different animals such as lions,jackals,hyenas,zebras,elephants,etc.Each year,grazing animals are infected and die from anthrax caused by the bacteria Bacillus anthracis.This increases the number of carcasses in the park,which serve as food for scavengers such as jackals.This study investigates the interplay between anthrax transmission in zebras and the scavenging of zebra carcasses in ENP,using a deterministic mathematical model to describe the population dynamics.We strive to answer the following research questions:Under what conditions can the presence of scavengers control anthrax outbreaks in zebra populations=Does carcass production by anthrax help or hurt scavengers in the long term?Standard qualitative analysis techniques distinguished outcomes(stable equilibria)using reproduction numbers as threshold quantities.We found that,when scavengers feed on anthrax-laden carcasses,the scavengers help the zebras,by eliminating potential infection zones for the zebras.In this way they reduce anthrax’s spread by orders of magnitude.We also identify conditions under which the presence of anthrax benefits the scavengers,in terms of death-to-birth ratios for zebras,scavengers and anthrax. 展开更多
关键词 Disease ecology ANTHRAX scavengers Demographic reproductive number Dynamical system
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Nanozyme‑Engineered Hydrogels for Anti‑Inflammation and Skin Regeneration
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作者 Amal George Kurian Rajendra K.Singh +2 位作者 Varsha Sagar Jung‑Hwan Lee Hae‑Won Kim 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第6期127-179,共53页
Inflammatory skin disorders can cause chronic scarring and functional impairments,posing a significant burden on patients and the healthcare system.Conventional therapies,such as corticosteroids and nonsteroidal anti-... Inflammatory skin disorders can cause chronic scarring and functional impairments,posing a significant burden on patients and the healthcare system.Conventional therapies,such as corticosteroids and nonsteroidal anti-inflammatory drugs,are limited in efficacy and associated with adverse effects.Recently,nanozyme(NZ)-based hydrogels have shown great promise in addressing these challenges.NZ-based hydrogels possess unique therapeutic abilities by combining the therapeutic benefits of redox nanomaterials with enzymatic activity and the water-retaining capacity of hydrogels.The multifaceted therapeutic effects of these hydrogels include scavenging reactive oxygen species and other inflammatory mediators modulating immune responses toward a pro-regenerative environment and enhancing regenerative potential by triggering cell migration and differentiation.This review highlights the current state of the art in NZ-engineered hydrogels(NZ@hydrogels)for anti-inflammatory and skin regeneration applications.It also discusses the underlying chemo-mechano-biological mechanisms behind their effectiveness.Additionally,the challenges and future directions in this ground,particularly their clinical translation,are addressed.The insights provided in this review can aid in the design and engineering of novel NZ-based hydrogels,offering new possibilities for targeted and personalized skin-care therapies. 展开更多
关键词 Nanozymes HYDROGELS ROS scavenging ANTI-INFLAMMATION Skin regeneration
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Integrated high-performance and accurate shaping technology of low-cost powder metallurgy titanium alloys: A comprehensive review
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作者 Xuemeng Gan Shaofu Li +1 位作者 Shunyuan Xiao Yafeng Yang 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2024年第3期413-426,共14页
The practical engineering applications of powder metallurgy (PM) Ti alloys produced through cold compaction and pressure-less sintering are impeded by poor sintering densification, embrittlement caused by excessive O ... The practical engineering applications of powder metallurgy (PM) Ti alloys produced through cold compaction and pressure-less sintering are impeded by poor sintering densification, embrittlement caused by excessive O impurities, and severe sintering deforma-tion resulting from the use of heterogeneous powder mixtures. This review presents a summary of our previous work on addressing the above challenges. Initially, we proposed a novel strategy using reaction-induced liquid phases to enhance sintering densification. Near- complete density (relative density exceeding 99%) was achieved by applying the above strategy and newly developed sintering aids. By focusing on the O-induced embrittlement issue, we determined the onset dissolution temperature of oxide films in the Ti matrix. On the basis of this finding, we established a design criterion for effective O scavengers that require reaction with oxide films before their dissol-ution. Consequently, a ductile PM Ti alloy was successfully obtained by introducing 0.3wt% NdB6 as the O scavenger. Lastly, a powder- coating strategy was adopted to address the sintering deformation issue. The ultrafine size and shell-like distribution characteristics of coating particles ensured rapid dissolution and homogeneity in the Ti matrix, thereby facilitating linear shrinkage during sintering. As a result, geometrically complex Ti alloy parts with high dimensional accuracy were fabricated by using the coated powder. Our fundament-al findings and related technical achievements enabled the development of an integrated production technology for the high-performance and accurate shaping of low-cost PM Ti alloys. Additionally, the primary engineering applications and progress in the industrialization practice of our developed technology are introduced in this review. 展开更多
关键词 powder metallurgy titanium sintering densification oxygen scavenging accurate shaping
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南、北五味子蛋白抗氧化活性和对HepG2细胞氧化应激损伤的修复作用
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作者 周泓妍 郑怡 +5 位作者 王海东 曹珺 张涵 张红印 李光哲 严铭铭 《食品与发酵工业》 CAS CSCD 2024年第7期51-60,共10页
采用碱提酸沉法提取南五味子蛋白(Schisandra sphenanthera protein,SSP)和北五味子蛋白(Schisandra chinensis protein,SCP),并测定其体外清除自由基能力、还原能力和对H_(2)O_(2)诱导的HepG2细胞氧化损伤的修复作用。采用CCK-8法检测... 采用碱提酸沉法提取南五味子蛋白(Schisandra sphenanthera protein,SSP)和北五味子蛋白(Schisandra chinensis protein,SCP),并测定其体外清除自由基能力、还原能力和对H_(2)O_(2)诱导的HepG2细胞氧化损伤的修复作用。采用CCK-8法检测细胞增殖率,荧光检测胞内活性氧水平,酶联免疫吸附法检测细胞氧化应激产物和抗氧化物酶系活性。结果表明,SSP清除自由基和还原能力均优于SCP,SSP与SCP都能对H_(2)O_(2)诱导HepG2造成的细胞生长抑制起到修复作用(P<0.05),显著降低胞内活性氧、丙二醛水平(P<0.05),显著提高超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和谷胱甘肽的水平(P<0.05),且SSP对H_(2)O_(2)诱导的HepG2氧化应激损伤修复能力优于SCP。综上,2种五味子蛋白中SSP更适合应用于抗氧化功能性食品或保健食品工业中。 展开更多
关键词 蛋白 自由基清除 氧化应激 HEPG2细胞 抗氧化
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风筛式油莎豆清选装置设计分析与试验
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作者 郑效帅 尚书旗 +5 位作者 王东伟 李成鹏 李明辉 申世龙 赵泽龙 何晓宁 《农机化研究》 2024年第5期146-153,159,共9页
作为一种新型油料作物,油莎豆清选损失率高、含杂率高的问题严重阻碍了其机械化收获进程。针对此问题,采用CFD-EDEM耦合方法进行了仿真试验、参数优化、规律解析和试验验证,结果表明:损失率影响程度从大到小为气流速度、振幅、频率,含... 作为一种新型油料作物,油莎豆清选损失率高、含杂率高的问题严重阻碍了其机械化收获进程。针对此问题,采用CFD-EDEM耦合方法进行了仿真试验、参数优化、规律解析和试验验证,结果表明:损失率影响程度从大到小为气流速度、振幅、频率,含杂率影响程度从大到小为气流速度、频率、振幅;气流速度为10.52m/s、振幅为20.61mm,振频为6.93Hz时,清选损失率为3.65%,含杂率为5.26%,清选综合性能指标最优为4.46%;台架性能试验损失率为3.77%,较仿真结果误差为3.19%;含杂率为5.52%,较仿真结果误差为2.84%;仿真试验误差较小,比较可靠。研究所得的最优工作参数组合可为油莎豆优质清选提供理论支撑。 展开更多
关键词 油莎豆 清选 流固耦合
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Study on Solid Fermentation and Antioxidant Function of Natto
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作者 Junxia SONG Hongbing QI Yanhong MAO 《Asian Agricultural Research》 2024年第1期32-36,共5页
[Objectives]To study the optimum conditions of solid fermentation of natto with antioxidant function as an index.[Methods]Single factor experiment and orthogonal experiment were designed to study the effects of temper... [Objectives]To study the optimum conditions of solid fermentation of natto with antioxidant function as an index.[Methods]Single factor experiment and orthogonal experiment were designed to study the effects of temperature,time,initial pH and inoculum amount on the antioxidant activity of natto solid fermentation.The optimum conditions of natto solid fermentation were determined and the antioxidant ac-tivity of natto extract was compared.[Results]The optimal fermentation conditions were as follows:temperature 32℃,initial pH 7.0,inocu-lation amount 8%,fermentation time 32 h.The hydroxyl radical scavenging rate of natto solid fermentation crude extract was the highest,which was 82.7%.The optimized nato fermentation extract showed stronger scavenging ability for-OH and O,:,and showed obvious dose-effect relationship.ICso was 3.63 and 4.24 mg/mL,respectively,and the scavenging efficiency was 1.3 and 1.9 times higher than that of the unoptimized fermentation extract,respectively.[Conclusions]Natto is rich in nattokinase and other functional factors,and its antioxidant ac-tivity can be improved by optimizing fermentation technology,so that natto products can be widely used,including cosmetic raw materials,nat-to skin care soap,health food and medicine,etc.,and have a broader development prospect. 展开更多
关键词 NATTO Solid fermentation Hydroxyl radical scavenging rate Antioxidant activity
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亚硝酸盐清除剂研究进展及其在肉制品中的应用
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作者 赵长青 代锦苹 +6 位作者 方春玉 刘世欣 朱荣贵 冉光雨 郭志强 周云川 赵志峰 《中国调味品》 CAS 2024年第1期198-202,220,共6页
亚硝酸盐在食品工业,尤其是在肉制品加工工业中具有抑菌、抗氧化、赋予肉制品特殊风味及发色等重要作用。然而,亚硝酸盐的摄入超出正常范围会造成身体组织的氧气不足,还可形成亚硝胺,最终引发癌症,但目前还未发现单一物质可替代亚硝酸... 亚硝酸盐在食品工业,尤其是在肉制品加工工业中具有抑菌、抗氧化、赋予肉制品特殊风味及发色等重要作用。然而,亚硝酸盐的摄入超出正常范围会造成身体组织的氧气不足,还可形成亚硝胺,最终引发癌症,但目前还未发现单一物质可替代亚硝酸盐。可见,有效控制肉制品中亚硝酸盐含量具有极其重要的意义。文章探讨了亚硝酸盐对食品安全的影响,通过对从蔬菜、水果等中提取的具有亚硝酸盐清除作用的天然物质及其在肉制品中的应用进行概述,为具有亚硝酸盐清除作用的物质在降低肉制品中亚硝酸盐含量的研究提供了理论借鉴。 展开更多
关键词 肉制品 亚硝酸盐 清除剂 食品安全
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Control of the Dust Vertical Distribution over Western Africa by Convection and Scavenging
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作者 H.Senghor R.Pilon +7 位作者 B.Diallo J.Escribano F.Hourdin J.Y.Grandpeix O.Boucher M.Gueye A.T.Gaye E.Machu 《Journal of Atmospheric Science Research》 2024年第1期19-39,共21页
Saharan dust represents more than 50%of the total desert dust emitted around the globe and its radiative effect significantly affects the atmospheric circulation at a continental scale.Previous studies on dust vertica... Saharan dust represents more than 50%of the total desert dust emitted around the globe and its radiative effect significantly affects the atmospheric circulation at a continental scale.Previous studies on dust vertical distribution and the Saharan Air Layer(SAL)showed some shortcomings that could be attributed to imperfect representation of the effects of deep convection and scavenging.The authors investigate here the role of deep convective transport and scavenging on the vertical distribution of mineral dust over Western Africa.Using multi-year(2006-2010)simulations performed with the variable-resolution(zoomed)version of the LMDZ climate model.Simulations are compared with aerosol amounts recorded by the Aerosol Robotic Network(AERONET)and with vertical profiles of the Cloud-Aerosol Lidar with Orthogonal Polarization(CALIOP)measurements.LMDZ allows a thorough examination of the respective roles of deep convective transport,convective and stratiform scavenging,boundary layer transport,and advection processes on the vertical mineral dust distribution over Western Africa.The comparison of simulated dust Aerosol Optical Depth(AOD)and distribution with measurements suggest that scavenging in deep convection and subsequent re-evaporation of dusty rainfall in the lower troposphere are critical processes for explaining the vertical distribution of desert dust.These processes play a key role in maintaining a well-defined dust layer with a sharp transition at the top of the SAL and in establishing the seasonal cycle of dust distribution.This vertical distribution is further reshaped offshore in the Inter-Tropical Convergence Zone(ITCZ)over the Atlantic Ocean by marine boundary layer turbulent and convective transport and wet deposition at the surface. 展开更多
关键词 DUST Vertical distribution Sahara SAHEL West Africa Climate model CONVECTION SCAVENGING ITCZ
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血清sCD163、CK18、25-(OH)D_(3)对肝硬化食管静脉曲张破裂出血患者医院感染风险的影响及预测价值
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作者 孙孟 王凤超 +3 位作者 贾静 刘义锋 王改存 俞致贤 《河南医学研究》 CAS 2024年第3期481-484,共4页
目的 探讨血清可溶性血红蛋白清道夫受体-163(sCD163)、细胞角蛋白18(CK18)、25-羟维生素D_(3)[25-(OH)D_(3)]对肝硬化合并食管静脉曲张破裂出血(EVB)患者医院感染风险的影响及预测价值。方法 选取南阳市中心医院2021年2月至2023年2月... 目的 探讨血清可溶性血红蛋白清道夫受体-163(sCD163)、细胞角蛋白18(CK18)、25-羟维生素D_(3)[25-(OH)D_(3)]对肝硬化合并食管静脉曲张破裂出血(EVB)患者医院感染风险的影响及预测价值。方法 选取南阳市中心医院2021年2月至2023年2月收治的114例肝硬化合并EVB患者,根据入院10 d内是否发生医院感染分为感染组(36例)和非感染组(78例)。比较两组血清sCD163、CK18、25-(OH)D_(3)水平,分析入院时血清sCD163、CK18、25-(OH)D_(3)水平与发生医院感染的相关性及影响因素,评价入院时血清sCD163、CK18、25-(OH)D_(3)水平联合检测对肝硬化合并EVB患者发生医院感染的预测价值。结果 入院时感染组血清sCD163、CK18水平较非感染组高,25-(OH)D_(3)水平较非感染组低(P<0.05);入院时血清CD163、CK18水平与发生医院感染均呈正相关,25-(OH)D_(3)水平与发生医院感染呈负相关(P<0.05);入院时血清sCD163(>5.16 mg·L^(-1))、CK18(>14.25μg·L^(-1))水平为发生医院感染的危险因素,25-(OH)D_(3)(>8.62μg·L^(-1))水平为发生医院感染的保护因素。入院时血清sCD163、CK18、25-(OH)D_(3)水平联合预测EVB患者发生医院感染的曲线下面积大于各单一指标(P<0.05)。结论 肝硬化合并EVB患者血清sCD163、CK18、25-(OH)D_(3)水平与医院感染有关,是影响医院感染的独立风险因素。 展开更多
关键词 肝硬化 食管静脉曲张破裂出血 医院感染 可溶性血红蛋白清道夫受体-163 细胞角蛋白18 25-羟维生素D_(3)
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高尿酸痛风患者血清sICAM-1、IL-6、sCD163水平的变化及临床意义
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作者 余玲 王秀成 +2 位作者 徐娜 张立赟 付文涛 《海南医学》 CAS 2024年第8期1140-1144,共5页
目的检测高尿酸痛风患者血清可溶性细胞间黏附分子-1(sICAM-1)、白细胞介素-6(IL-6)、可溶性单核巨噬细胞血红蛋白清道夫受体(sCD163)水平并探讨其临床意义。方法选取2020年1月至2023年1月南阳豫西健和医院收治的200例高尿酸血症(HUA)... 目的检测高尿酸痛风患者血清可溶性细胞间黏附分子-1(sICAM-1)、白细胞介素-6(IL-6)、可溶性单核巨噬细胞血红蛋白清道夫受体(sCD163)水平并探讨其临床意义。方法选取2020年1月至2023年1月南阳豫西健和医院收治的200例高尿酸血症(HUA)患者纳入研究,其中92例痛风患者作为观察组,108例无症状患者作为对照组,所有患者均检测血清sICAM-1、IL-6、sCD163水平及血尿酸含量。比较两组患者的血清sICAM-1、IL-6、sCD163水平及血尿酸含量。采用Pearson相关性、多元线性回归分析血清sICAM-1、IL-6、sCD163水平与血尿酸含量的相关性。采用相对危险度(RR)分析血清sICAM-1、IL-6、sCD16对高尿酸痛风发病风险的影响,绘制受试者工作特征曲线(ROC)评价血清sICAM-1、IL-6、sCD163对高尿酸痛风的诊断价值。结果观察组患者的血清sICAM-1、IL-6、sCD163水平和血尿酸含量分别为(574.26±95.22)ng/mL、(50.12±8.27)ng/L、(825.83±198.22)pg/mL、(582.37±156.92)μmol/L,明显高于对照组的(401.47±42.83)ng/mL、(39.82±6.13)ng/L、(694.28±114.83)pg/mL、(425.77±114.83)μmol/L,差异均有统计学意义(P<0.05);经Pearson相关性分析结果显示,高尿酸痛风患者血清sICAM-1、IL-6、sCD163水平与血尿酸水平呈显著正相关(P<0.05);经线性回归建立回归模型分析结果显示,高尿酸痛风患者血清sICAM-1、IL-6、sCD163水平与血尿酸水平呈极显著相关(P<0.01);HUA患者血清sICAM-1≥483.41 ng/mL、IL-6≥44.70 ng/L、sCD16≥598.93 pg/mL时,高尿酸痛风发病风险分别是血清sICAM-1<483.41 ng/mL、IL-6<44.70 ng/L、sCD16<598.93 pg/mL的5.133倍(95%CI:3.181~8.285)、4.412倍(95%CI:2.820~6.903)、3.600倍(95%CI:2.388~5.428);经ROC分析结果显示,血清sICAM-1、IL-6、sCD163诊断高尿酸痛风的曲线下面积(AUC)分别为0.775、0.731、0.792,进一步分析结果显示,血清sICAM-1、IL-6、sCD163联合诊断AUC为0.940,明显较各时间点单个指标大(P<0.05)。结论高尿酸痛风患者血清sICAM-1、IL-6、sCD163明显升高,且与血尿酸密切相关,临床检测其水平有助于疾病诊断。 展开更多
关键词 高尿酸血症 痛风 可溶性细胞间黏附分子-1 白细胞介素-6 可溶性单核巨噬细胞血红蛋白清道夫受体 相关性
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小麦胚芽肽和大豆酶解蛋白的体外抗氧化活性研究
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作者 边智尧 陈宝江 +2 位作者 申帅峰 随腾玖 韩帅娟 《饲料工业》 CAS 2024年第7期80-86,共7页
试验旨在研究小麦胚芽肽和大豆酶解蛋白的分子质量分布和体外抗氧化活性。试验采用单因素完全随机设计,以维生素C为对照,分别测定小麦胚芽肽和大豆酶解蛋白在0.625、1.25、2.5、5、10 mg/mL浓度下对DPPH自由基、羟自由基和2,2-联氮-二(3... 试验旨在研究小麦胚芽肽和大豆酶解蛋白的分子质量分布和体外抗氧化活性。试验采用单因素完全随机设计,以维生素C为对照,分别测定小麦胚芽肽和大豆酶解蛋白在0.625、1.25、2.5、5、10 mg/mL浓度下对DPPH自由基、羟自由基和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基的清除率,并通过测定T-AOC吸光度值计算总还原力。结果表明:使用高效液相色谱测定小麦胚芽肽和大豆酶解蛋白重均分子质量分别为513 u和551 u,二者分子质量小于1000 u的比例分别为90.22%和86.93%。在一定浓度范围内,随小麦胚芽肽和大豆酶解蛋白浓度的升高,其体外抗氧化性能线性增加(P<0.05),且呈正相关关系。在10 mg/mL浓度时,小麦胚芽肽对DPPH自由基、羟自由基和ABTS自由基清除率和总还原力分别为90.39%、70.66%、96.24%和2.23,大豆酶解蛋白对DPPH自由基、羟自由基和ABTS自由基清除率和总还原力分别达到65.55%、68.21%、75.14%和0.51。综上,在本试验条件下,不同浓度小麦胚芽肽和大豆酶解蛋白均具有一定的抗氧化能力,且小麦胚芽肽相较于大豆酶解蛋白的抗氧化能力更强。 展开更多
关键词 小麦胚芽肽 大豆酶解蛋白 体外抗氧化性能 总还原力 自由基清除率
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清道夫受体⁃A在类风湿关节炎中的作用及其应用研究进展
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作者 马玲萍 张媛媛 +2 位作者 许亚丽 王嘉 王钢 《中国骨质疏松杂志》 CAS CSCD 2024年第3期441-446,共6页
清道夫受体(scavenger receptors,SR)是表达于髓样细胞上功能多样的蛋白质,不仅具有清除修饰脂蛋白、识别并结合配体的能力,而且作为先天模式识别受体之一,在生物学过程中发挥着重要作用。因此,SR不仅对于维持宿主体内平衡起着关键作用... 清道夫受体(scavenger receptors,SR)是表达于髓样细胞上功能多样的蛋白质,不仅具有清除修饰脂蛋白、识别并结合配体的能力,而且作为先天模式识别受体之一,在生物学过程中发挥着重要作用。因此,SR不仅对于维持宿主体内平衡起着关键作用,而且还参与了许多疾病的发病机制。类风湿关节炎(rheumatoid arthritis,RA)的特征是炎性细胞浸润和关节滑膜炎的发生,滑膜炎中最显著的细胞是巨噬细胞,活化的巨噬细胞能够产生多种细胞因子,最终导致软骨和骨的严重破坏。近期研究发现,SR⁃A作为SR的分类之一,在RA的发病过程中起着关键性作用,本文就SR⁃A在RA中的作用及其应用研究进展作一综述,以期能够为临床诊断及治疗RA提供新思路、新方法。 展开更多
关键词 清道夫受体⁃A 类风湿关节炎 研究进展
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基于循环超声提取枇杷三萜酸工艺优化及其抗氧化性分析
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作者 郭明遗 李密 +2 位作者 赵志平 吴华昌 邓艳 《食品研究与开发》 CAS 2024年第1期107-114,共8页
采用响应面法优化循环超声提取枇杷果肉中的三萜酸的工艺条件,在单因素基础上,选择液料比、乙醇浓度、循环转速、超声时间为影响因素,以三萜酸提取量为指标进行响应面分析,并研究三萜酸提取物的抗氧化活性。结果表明,最佳提取工艺参数... 采用响应面法优化循环超声提取枇杷果肉中的三萜酸的工艺条件,在单因素基础上,选择液料比、乙醇浓度、循环转速、超声时间为影响因素,以三萜酸提取量为指标进行响应面分析,并研究三萜酸提取物的抗氧化活性。结果表明,最佳提取工艺参数为液料比15.5∶1(mL/g)、乙醇浓度70.0%、循环转速811 r/min、超声时间43.5 min,在该条件下三萜酸提取量为(1.789±0.080)mg/g;在三萜酸提取物浓度为0.48 mg/mL时,对ABTS^(+)自由基、DPPH自由基、·OH、·O_(2)^(-)的清除率分别达到60.11%、77.86%、52.76%、45.34%。对4种自由基的半抑制浓度(IC_(50))分别为0.148、0.102、0.382、0.510 mg/mL。 展开更多
关键词 循环超声 枇杷 三萜酸 抗氧化活性 清除率
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抗氧化剂筛选复配及在咖啡饮料中的应用研究
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作者 常旋 徐春明 +7 位作者 陈佳 张佳靓 葛丽霞 阳丽红 王根女 王旭捷 张鑫 陈雄 《食品与发酵工业》 CSCD 2024年第1期148-154,共7页
为开发可直接用于饮料体系的抗氧化剂,该研究探究了不同水溶性和脂溶性抗氧化剂的DPPH自由基清除率,对椰子油乳液氧化稳定性的影响,复配抗氧化剂稳定性和在饮料中的应用。结果表明,抗氧化剂的DPPH清除率和油脂氧化稳定性没有呈现正相关... 为开发可直接用于饮料体系的抗氧化剂,该研究探究了不同水溶性和脂溶性抗氧化剂的DPPH自由基清除率,对椰子油乳液氧化稳定性的影响,复配抗氧化剂稳定性和在饮料中的应用。结果表明,抗氧化剂的DPPH清除率和油脂氧化稳定性没有呈现正相关。水溶性抗氧化剂中效果较好的是竹叶黄酮和甘草提取物,脂溶性抗氧化剂中效果较好的是二丁基羟基对甲酚、特丁基对苯二酚和维生素E。以贮藏期间粒径变化为指标,探究了复配抗氧化剂的稳定性。结果表明,维生素E和甘草提取物、维生素E和竹叶黄酮复配抗氧化剂在55℃贮藏8周期间粒径D[4,3]和粒径分布没有变化。以咖啡饮料为模型,研究了复配抗氧化剂对风味稳定性的影响。结果表明,与单一抗氧化剂相比,复配抗氧化剂的加入更明显地减少了咖啡中油脂的氧化产物堆积。感官评价结果表明,复配抗氧化剂的加入抑制了油脂氧化异味的产生,香气更加协调,总体接受度最高。 展开更多
关键词 DPPH自由基清除率 抗氧化剂 稳定性 咖啡 感官评价
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4种不同来源花青素的稳定性及抗氧化性比较
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作者 马孟佳 赵娟 +2 位作者 陈鹏飞 陈祥贵 黄玉坤 《食品研究与开发》 CAS 2024年第4期31-39,67,共10页
为更好地探索不同原料的花青素作为食品着色剂和营养剂的潜力,对蓝莓、紫薯、紫甘蓝、黑豆的花青素进行提取,对4种原料花青素含量进行比较,并探究4种原料花青素的抗氧化性以及在不同因素下(光照、pH值、温度、金属离子、氧化剂、还原剂... 为更好地探索不同原料的花青素作为食品着色剂和营养剂的潜力,对蓝莓、紫薯、紫甘蓝、黑豆的花青素进行提取,对4种原料花青素含量进行比较,并探究4种原料花青素的抗氧化性以及在不同因素下(光照、pH值、温度、金属离子、氧化剂、还原剂、葡萄糖浓度、蛋白质)的稳定性。结果表明,花青素含量排序为紫甘蓝(435.84 mg/L)>紫薯(401.77 mg/L)>蓝莓(388.08 mg/L)>黑豆(177.34 mg/L)。稳定性最佳的原料花青素为紫甘蓝花青素,抗氧化性最强的为蓝莓花青素(羟自由基清除率、DPPH自由基清除率、ABTS^(+)自由基清除率分别为76.41%、83.05%、88.13%,总还原能力测得吸光值为2.04)。 展开更多
关键词 花青素 色素保存率 稳定性 自由基清除率 抗氧化性
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