BACKGROUND Fourth degree burns damage the full thickness of the skin and affect underlying tissues.Skin grafting after debridement is often used to cover the wounds of salvageable severe burns.A granulation wound can ...BACKGROUND Fourth degree burns damage the full thickness of the skin and affect underlying tissues.Skin grafting after debridement is often used to cover the wounds of salvageable severe burns.A granulation wound can be formed by drilling the skull to the barrier layer to solve the problem of skull exposure.Low oxygen levels present at high altitudes aggravate ischemia and hypoxia which can negatively impact wound healing.The impaired healing in such cases can be ameliorated by hyperbaric oxygen therapy.CASE SUMMARY We describe a patient who presented with fourth degree burns to the left temporal and facial regions upon admission in December 2018.The periosteum of the skull and the deep fascia of the face were exposed.After the first stage of debridement and skin grafting,the temporal skin did not survive well.Granulation was induced by cranial drilling,and then a local flap was transferred to cover the wound.The left temporal and facial wounds were completely covered and the patient recovered well.CONCLUSION Skin grafting and flap transfer after early debridement to cover the wound and control infection were of great significance.In the later stages of the patient's treatment,survival of the skin graft and skin flap was observed.The second stage repair was performed to achieve successful skin grafting by cranial granulation.Granulation was formed by drilling the skull,and then the wound was closed,which is suitable for cases with skull exposure and wounds with poor blood supply.We consider that hyperbaric oxygen treatment and improving tissue oxygen supply were beneficial in this patient.展开更多
AIM:To assess feasibility of a finger prick-based kit as method for self-testing of first and second-degree relatives of coeliac disease (CD) patients. METHODS:A total number of 379 subjects were invited to participat...AIM:To assess feasibility of a finger prick-based kit as method for self-testing of first and second-degree relatives of coeliac disease (CD) patients. METHODS:A total number of 379 subjects were invited to participate in this study,consisting of 197 first- degree and 182 second-degree relatives of CD patients. The self-testing kit (BiocardTM) was sent out with included instructions for use. Completed tests were sent back to the study coordinator for assessment. RESULTS:One hundred and ninety-six invited relatives carried out the BiocardTM test at home. Amongst these,70% were children. In 97% of the cases the test was performed correctly. Three tests revealed a positive result,all of which were later confirmed by serology and histology as coeliac disease.CONCLUSION:Our study indicates that BiocardTM test is a reliable,easy to use and well-accepted tool for home testing of first- and second-degree relatives of CD patients.展开更多
目的:基于网络药理学和分子对接方法,确定复方黄柏液治疗的Ⅲ度烧伤肉芽组织愈合的有效活性成分、关键靶点和潜在的药理学机制,并进行肉芽组织成纤维细胞的初步验证。方法:从公共数据库中药系统药理学分析平台(TCMSP)检索复方黄柏液组...目的:基于网络药理学和分子对接方法,确定复方黄柏液治疗的Ⅲ度烧伤肉芽组织愈合的有效活性成分、关键靶点和潜在的药理学机制,并进行肉芽组织成纤维细胞的初步验证。方法:从公共数据库中药系统药理学分析平台(TCMSP)检索复方黄柏液组成成分连翘、黄柏、金银花的有效成分和靶点;GeneCards、OMIM数据库检索“Ⅲ度烧伤”疾病相关靶点。通过生物信息学分析,包括蛋白质-蛋白质相互作用(Protein-proteininteraction,PPI)以及基因本体(Gene ontology,GO)和京都基因和基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)分析,获得了关键的有效成分、核心靶点和相关信号通路;DiscoveryStudio分子对接分析有效成分化合物与靶蛋白的结合。0.5%的DMSO溶液处理的成纤维细胞记为对照组;槲皮素(40μmol/ml)处理的成纤维细胞记为槲皮素组。采用CCK8法、Transwell实验检测细胞增殖、迁移侵袭;WB试验检测细胞p-PI3K、p-Akt蛋白。结果:共筛选出74个有效成分,331个作用靶点,AKT1为潜在的治疗靶点,木犀草素、山柰酚、槲皮素、汉黄芩素、丹皮酚为潜在的候选药物。PI3K-AKT信号通路可能在复方黄柏液治疗Ⅲ度烧伤中发挥关键作用;分子对接表明槲皮素与AKT1结合最好。与对照组相比,槲皮素组成纤维细胞增殖、迁移侵袭均显著降低,p-PI3K、p-Akt蛋白表达也显著降低(P<0.05)。结论:复方黄柏液促进Ⅲ度烧伤患者肉芽组织形成的生物活性成分为槲皮素,潜在通路为PI3K-AKT信号通路,为复方黄柏液治疗Ⅲ度烧伤的研究提供了思路。展开更多
目的探讨自体富血小板凝胶(APG)结合封闭负压引流(VSD)对深Ⅱ度烧伤患者创面愈合进程及表皮生长因子(EGF)、碱性成纤维细胞生长因子(bFGF)水平的影响。方法选取2018年7月—2021年7月在南通大学附属南通第三医院就诊的82例深Ⅱ度烧伤患...目的探讨自体富血小板凝胶(APG)结合封闭负压引流(VSD)对深Ⅱ度烧伤患者创面愈合进程及表皮生长因子(EGF)、碱性成纤维细胞生长因子(bFGF)水平的影响。方法选取2018年7月—2021年7月在南通大学附属南通第三医院就诊的82例深Ⅱ度烧伤患者作为研究对象,按随机数字表法分为观察组(APG结合VSD治疗)和对照组(VSD治疗),各41例。比较两组患者的疗效、创面愈合时间、肉芽生长面积、创面愈合率、住院时间、并发症,比较两组患者治疗前后的EGF、bFGF、C反应蛋白(CRP)、白细胞介素6(IL-6)、白细胞计数(WBC)、疼痛视觉模拟评分(VAS)的差值及治疗后1个月、2个月的温哥华瘢痕量表(VSS)评分的差值。结果观察组总有效率高于对照组(P<0.05)。观察组创面愈合时间、住院时间少于对照组,肉芽生长面积大于对照组,创面愈合率高于对照组(P<0.05)。观察组治疗前后EGF、bFGF、CRP、IL-6、WBC的差值高于对照组(P<0.05)。观察组治疗前与治疗后14 d VAS评分及治疗后1个月与2个月VSS评分的差值高于对照组(P<0.05)。两组患者并发症总发生率比较,差异无统计学意义(P>0.05)。结论APG结合VSD治疗深Ⅱ度烧伤可加快患者创面愈合进程,提高EGF、bFGF水平,减轻炎症反应和疼痛,减少瘢痕增生,且并发症少。展开更多
The issue of designing a type of generalized Luenberger observers for matrix second-order linear (MSOL) systems was addressed in the matrix second-order framework. By introducing the concept of stable matrix pair for ...The issue of designing a type of generalized Luenberger observers for matrix second-order linear (MSOL) systems was addressed in the matrix second-order framework. By introducing the concept of stable matrix pair for MSOL systems, sufficient and necessary conditions for the design of the type of generalized Luenberger observers were given under the assumption of controllability and observability of the MSOL system. Based on the proposed conditions and the right coprime factorization of the system, a parametric approach to the design of such type of observers was presented. The proposed approach provides all the degrees of design freedom, which can be further utilized to achieve additional system specifications. A spring-mass system was utilized to show the effect of the proposed method.展开更多
文摘BACKGROUND Fourth degree burns damage the full thickness of the skin and affect underlying tissues.Skin grafting after debridement is often used to cover the wounds of salvageable severe burns.A granulation wound can be formed by drilling the skull to the barrier layer to solve the problem of skull exposure.Low oxygen levels present at high altitudes aggravate ischemia and hypoxia which can negatively impact wound healing.The impaired healing in such cases can be ameliorated by hyperbaric oxygen therapy.CASE SUMMARY We describe a patient who presented with fourth degree burns to the left temporal and facial regions upon admission in December 2018.The periosteum of the skull and the deep fascia of the face were exposed.After the first stage of debridement and skin grafting,the temporal skin did not survive well.Granulation was induced by cranial drilling,and then a local flap was transferred to cover the wound.The left temporal and facial wounds were completely covered and the patient recovered well.CONCLUSION Skin grafting and flap transfer after early debridement to cover the wound and control infection were of great significance.In the later stages of the patient's treatment,survival of the skin graft and skin flap was observed.The second stage repair was performed to achieve successful skin grafting by cranial granulation.Granulation was formed by drilling the skull,and then the wound was closed,which is suitable for cases with skull exposure and wounds with poor blood supply.We consider that hyperbaric oxygen treatment and improving tissue oxygen supply were beneficial in this patient.
基金Supported by Teddy Schwarzohr Verein zur Unterstützung von chronisch kranken Kindern
文摘AIM:To assess feasibility of a finger prick-based kit as method for self-testing of first and second-degree relatives of coeliac disease (CD) patients. METHODS:A total number of 379 subjects were invited to participate in this study,consisting of 197 first- degree and 182 second-degree relatives of CD patients. The self-testing kit (BiocardTM) was sent out with included instructions for use. Completed tests were sent back to the study coordinator for assessment. RESULTS:One hundred and ninety-six invited relatives carried out the BiocardTM test at home. Amongst these,70% were children. In 97% of the cases the test was performed correctly. Three tests revealed a positive result,all of which were later confirmed by serology and histology as coeliac disease.CONCLUSION:Our study indicates that BiocardTM test is a reliable,easy to use and well-accepted tool for home testing of first- and second-degree relatives of CD patients.
文摘目的:基于网络药理学和分子对接方法,确定复方黄柏液治疗的Ⅲ度烧伤肉芽组织愈合的有效活性成分、关键靶点和潜在的药理学机制,并进行肉芽组织成纤维细胞的初步验证。方法:从公共数据库中药系统药理学分析平台(TCMSP)检索复方黄柏液组成成分连翘、黄柏、金银花的有效成分和靶点;GeneCards、OMIM数据库检索“Ⅲ度烧伤”疾病相关靶点。通过生物信息学分析,包括蛋白质-蛋白质相互作用(Protein-proteininteraction,PPI)以及基因本体(Gene ontology,GO)和京都基因和基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)分析,获得了关键的有效成分、核心靶点和相关信号通路;DiscoveryStudio分子对接分析有效成分化合物与靶蛋白的结合。0.5%的DMSO溶液处理的成纤维细胞记为对照组;槲皮素(40μmol/ml)处理的成纤维细胞记为槲皮素组。采用CCK8法、Transwell实验检测细胞增殖、迁移侵袭;WB试验检测细胞p-PI3K、p-Akt蛋白。结果:共筛选出74个有效成分,331个作用靶点,AKT1为潜在的治疗靶点,木犀草素、山柰酚、槲皮素、汉黄芩素、丹皮酚为潜在的候选药物。PI3K-AKT信号通路可能在复方黄柏液治疗Ⅲ度烧伤中发挥关键作用;分子对接表明槲皮素与AKT1结合最好。与对照组相比,槲皮素组成纤维细胞增殖、迁移侵袭均显著降低,p-PI3K、p-Akt蛋白表达也显著降低(P<0.05)。结论:复方黄柏液促进Ⅲ度烧伤患者肉芽组织形成的生物活性成分为槲皮素,潜在通路为PI3K-AKT信号通路,为复方黄柏液治疗Ⅲ度烧伤的研究提供了思路。
文摘目的探讨自体富血小板凝胶(APG)结合封闭负压引流(VSD)对深Ⅱ度烧伤患者创面愈合进程及表皮生长因子(EGF)、碱性成纤维细胞生长因子(bFGF)水平的影响。方法选取2018年7月—2021年7月在南通大学附属南通第三医院就诊的82例深Ⅱ度烧伤患者作为研究对象,按随机数字表法分为观察组(APG结合VSD治疗)和对照组(VSD治疗),各41例。比较两组患者的疗效、创面愈合时间、肉芽生长面积、创面愈合率、住院时间、并发症,比较两组患者治疗前后的EGF、bFGF、C反应蛋白(CRP)、白细胞介素6(IL-6)、白细胞计数(WBC)、疼痛视觉模拟评分(VAS)的差值及治疗后1个月、2个月的温哥华瘢痕量表(VSS)评分的差值。结果观察组总有效率高于对照组(P<0.05)。观察组创面愈合时间、住院时间少于对照组,肉芽生长面积大于对照组,创面愈合率高于对照组(P<0.05)。观察组治疗前后EGF、bFGF、CRP、IL-6、WBC的差值高于对照组(P<0.05)。观察组治疗前与治疗后14 d VAS评分及治疗后1个月与2个月VSS评分的差值高于对照组(P<0.05)。两组患者并发症总发生率比较,差异无统计学意义(P>0.05)。结论APG结合VSD治疗深Ⅱ度烧伤可加快患者创面愈合进程,提高EGF、bFGF水平,减轻炎症反应和疼痛,减少瘢痕增生,且并发症少。
文摘The issue of designing a type of generalized Luenberger observers for matrix second-order linear (MSOL) systems was addressed in the matrix second-order framework. By introducing the concept of stable matrix pair for MSOL systems, sufficient and necessary conditions for the design of the type of generalized Luenberger observers were given under the assumption of controllability and observability of the MSOL system. Based on the proposed conditions and the right coprime factorization of the system, a parametric approach to the design of such type of observers was presented. The proposed approach provides all the degrees of design freedom, which can be further utilized to achieve additional system specifications. A spring-mass system was utilized to show the effect of the proposed method.
