Underground pipeline networks constitute a major component of urban infrastructure,and thus,it is imperative to have an efficient mechanism to manage them.This study introduces a secondary development system to effici...Underground pipeline networks constitute a major component of urban infrastructure,and thus,it is imperative to have an efficient mechanism to manage them.This study introduces a secondary development system to efficiently model underground pipeline networks,using the building information modeling(BIM)-based software Revit.The system comprises separate pipe point and tubulation models.Using a Revit application programming interface(API),the spatial position and attribute data of the pipe points are extracted from a pipeline database,and the corresponding tubulation data are extracted from a tubulation database.Using the Family class in Revit API,the cluster in the self-built library of pipe point is inserted into the spatial location and the attribute data is added;in the same way,all pipeline instances in the pipeline system are created.The extension and localization of the model accelerated the modeling speed.The system was then used in a real construction project.The expansion of the model database and rapid modeling made the application of BIM technology in three-dimensional visualization of underground pipeline networks more convenient.Furthermore,it has applications in pipeline engineering construction and management.展开更多
The platform with bucket foundations can penetrate and migrate by underpressure/positive pressure technique caused by pumping water out/in the bucket. However, the construction process of bucket foundations cannot be ...The platform with bucket foundations can penetrate and migrate by underpressure/positive pressure technique caused by pumping water out/in the bucket. However, the construction process of bucket foundations cannot be clearly observed and effectively controlled due to the special nature of sea environment. By using an advanced simulation development tool of Multigen Creator, the visual construction simulation program for the platform with bucket foundations was developed to set up the virtual reality system with interaction control and observation in every view angle based on the secondary development technology of Vega platform. The application results show that the method is feasible and effective by simulating the whole construction process for the platform with four bucket foundations.展开更多
This paper develops a strong secondary development based on ADAMS feature which creates high-speed rail bearings for simulation analysis module. This thesis is in the case of non-circular pattern instructions of how t...This paper develops a strong secondary development based on ADAMS feature which creates high-speed rail bearings for simulation analysis module. This thesis is in the case of non-circular pattern instructions of how to achieve rapid roller modeling, with analysis of functions and parameters required for the design of the simulation module of the high-speed rail bearing, as well as the design of dialog boxes, the environment and file structure. The specific modules is based on the secondary development language provided by ADAMS ! View. Through the menus, dialog boxes which input parameters, it can achieve high iron bearing automatic modeling, dynamic analysis and post-processing to simplify the analysis of high-speed rail bearing operations, as well as improving the high-speed rail bearing development efficiency.展开更多
In order to shorten the design cycle of the excavator working device, we have proposed a rapid modeling method for the excavator working device which uses parameters. Based on the Pro/toolkit, which is secondary devel...In order to shorten the design cycle of the excavator working device, we have proposed a rapid modeling method for the excavator working device which uses parameters. Based on the Pro/toolkit, which is secondary development tool of Pro/E4.0,and combined with Vs C＋＋2005 programming software. It developed a flexible set of MFC visualization-friendly interfaces. Users can enter data in the visual interface according to their needs and it will generate a new part model quickly. So it improves the design quality, shortens the design cycle, and makes the cost lower significantly.展开更多
The creep characteristics of rock under high crustal stress are of important influence on the long‐term stability of deep rock engineering.To study the creep characteristics and engineering application of sandstone u...The creep characteristics of rock under high crustal stress are of important influence on the long‐term stability of deep rock engineering.To study the creep characteristics and engineering application of sandstone under high crustal stress,this study constructed nonlinear creep damage(NCD)constitutive mode based on the triaxial graded loading‒unloading creep test of sandstone in the Yuezhishan Tunnel.A numerical NCD constitutive model and a breakable lining(BL)model were developed based on FLAC3D and then applied to the stability analysis of the Yuezhishan Tunnel.Based on the creep test results of sandstone,a power function of creep rate and stress level was constructed,by which the long‐term strength was solved.The results show that the long‐term strength of the red sandstone based on the related function of the steady‐state creep rate and stress level is close to the measured stress value in engineering.The NCD model considering damage factors reflects the instantaneous and viscoelastic plasticity deformation characteristics of the red sandstone.The numerical NCD constitutive model and the BL model can reflect surrounding rock deformation characteristics and lining failure characteristics in practical engineering.The research results provide theoretical references for long‐term stability analysis of rock engineering and the deformation control of surrounding rock under high crustal stress.展开更多
In order to study the spatial-temporal change and environmental management of regional karst LUCC (land use and land cover change) and its causative environmental effect-rocky desertification by integrating qualitativ...In order to study the spatial-temporal change and environmental management of regional karst LUCC (land use and land cover change) and its causative environmental effect-rocky desertification by integrating qualitative analysis and quantitative analysis, and relying on RS, GIS and GPS (3S) techniques, karst land rocky derification dynamic monitoring and visualization management information system (KLRD.DMVM.IS) is framed, which includes design aim and structure model, function design, database design and model system design. The model system design gives priority to dynamic monitoring, drive force diagnosis, comprehensive evaluation and decision support of karst rocky desertification. From the viewpoint of model type, mathematic expression and its meaning, the dynamic monitoring models are concretely devised to reflect the spatial and temporal changing features and the trend of karst LUCC and rocky desertification. Taking Du'an Yao Autonomic County of Guangxi as an example, the KLRD.DMVM.IS is systematically analyzed in the application of the process and trend of karst LUCC and rocky desertification in Du'an County, and it provides the technical support for the study on karst land rocky desertification.展开更多
Although poplar is widely used for genomic and biotechnological manipulations of wood, the cellular basis of wood development in poplar has not been accurately documented at an ultrastructural level. Developing second...Although poplar is widely used for genomic and biotechnological manipulations of wood, the cellular basis of wood development in poplar has not been accurately documented at an ultrastructural level. Developing secondary xylem cells from hybrid poplar （Populus deltoides x P. trichocarpa）, which were actively making secondary cell walls, were preserved with high pressure freezing/freeze substitution for light and electron microscopy. The distribution of xylans and mannans in the different cell types of developing secondary xylem were detected with immunofluorescence and immuno-gold labeling. While xylans, detected with the monoclonal antibody LM10, had a general distribution across the secondary xylem, mannans were enriched in the S2 secondary cell wall layer of fibers. To observe the cellular structures associated with secondary wall production, cryofixed fibers were examined with transmission electron microscopy during differentiation. There were abundant cortical microtubules and endomembrane activity in cells during the intense phase of secondary cell wall synthesis. Microtubuleassociated small membrane compartments were commonly observed, as well as Golgi and secretory vesicles fusing with the plasma membrane.展开更多
Interval analysis is a new uncertainty analysis method for engineering structures. In this paper, a new sensitivity analysis method is presented by introducing interval analysis which can expand applications of the in...Interval analysis is a new uncertainty analysis method for engineering structures. In this paper, a new sensitivity analysis method is presented by introducing interval analysis which can expand applications of the interval analysis method. The interval analysis process of sensitivity factor matrix of soil parameters is given. A method of parameter intervals and decision-making target intervals is given according to the interval analysis method. With FEM, secondary developments are done for Marc and the Duncan-Chang nonlinear elastic model. Mutual transfer between FORTRAN and Marc is implemented. With practial examples, rationality and feasibility are validated. Comparison is made with some published results.展开更多
This paper focuses on the problem of low efficiency and limited non-geometric information handling ability in the process of 2D heating furnace design, proposes a 3D concurrent parametric design system, this method is...This paper focuses on the problem of low efficiency and limited non-geometric information handling ability in the process of 2D heating furnace design, proposes a 3D concurrent parametric design system, this method is realized by parametric design technology, which is supported by ActiveX-Automation technology and VBA technology, mutual visit between application programs makes batched modeling become possible, key dimensions are linked with each other by restrictions, so parts can be built concurrently by sharing few parameters between common borders, designer can be free from repeated drawing work during modification, Solid Edge is chosen as the modeling server, a secondary development software is programmed by Visual Basic, this system provides a feasible way to overlap time between different sections, the design efficiency and quality is improved significantly.展开更多
Secondary vascular development is a key biological characteristic of woody plants and the basis of wood formation.Our understanding of gene expression regulation and dynamic changes in microRNAs(miRNAs)during secondar...Secondary vascular development is a key biological characteristic of woody plants and the basis of wood formation.Our understanding of gene expression regulation and dynamic changes in microRNAs(miRNAs)during secondary vascular development is still limited.Here we present an integrated analysis of the miRNA and mRNA transcriptome of six phase-specific tissues-the shoot apex,procambium,primary vascular tissue,cambium,secondary phloem,and secondary xylem-in Populus tomentosa.Several novel regulatory modules,including the PtoTCP20-miR396d-PtoGRF15 module,were identified during secondary vascular development in Populus.A series of biochemical and molecular experiments confirmed that PtoTCP20 activated transcription of the miR396d precursor gene and that miR396d targeted PtoGRF15 to downregulate its expression.Plants overexpressing miR396d(35S:miR396d)showed enhanced secondary growth and increased xylem production.Conversely,during the transition from primary to secondary vascular development,plants with downregulated PtoTCP20expression(PtoTCP20-SRDX),downregulated miR396 expression(35S:STTM396),and PtoGRF15 overexpression(35S:PtoGRF15)showed delayed secondary growth.Novel regulatory modules were identified by integrated analysis of the miRNA and mRNA transcriptome,and the regulatory role of the PtoTCP20-miR396d-PtoGRF15 signaling cascade in secondary vascular development was validated in Populus,providing information to support improvements in forest cultivation and wood properties.展开更多
The materials with different moduli in tension and compression are called bi-modulus materials. Graphene is such a kind of materials with the highest strength and the thinnest thickness. In this paper, the mechanical ...The materials with different moduli in tension and compression are called bi-modulus materials. Graphene is such a kind of materials with the highest strength and the thinnest thickness. In this paper, the mechanical response of the bi-modulus beam subjected to the temperature effect and placed on the Winkler foundation is studied. The differential equations about the neutral axis position and undetermined parameters of the normal strain of the bi-modulus foundation beam are established. Then, the analytical expressions of the normal stress, bending moment, and displacement of the foundation beam are derived. Simultaneously, a calculation procedure based on the finite element method （FEM） is developed to obtain the temperature stress of the bi-modulus struc- tures. It is shown that the obtained bi-modulus solutions can recover the classical modulus solution, and the results obtained by the analytical expressions, the present FEM proce- dure, and the traditional FEM software are consistent, which verifies the accuracy and reliability of the present analytical model and procedure. Finally, the difference between the bi-modulus results and the classical same modulus results is discussed, and several reasonable suggestions for calculating and optimizing the certain bi-modulus member in practical engineering are presented.展开更多
GC-GIS system is a geochemical data processing system based on fractal theory. The system realized quantity statistics function by calling Surfer and MapInfo software, and it is compiled with Visual Basic language. Th...GC-GIS system is a geochemical data processing system based on fractal theory. The system realized quantity statistics function by calling Surfer and MapInfo software, and it is compiled with Visual Basic language. This system is designed to integrate the functions both quantity statistics of Surfer and spatial data management of MapInfo. A new algorithm of fractal is added up to GC-GIS. Taking example for Weichang region of Hebei to test the system, the processing results show that the model can match the real distribution of mine well.展开更多
Combining with engineering examples,this paper introduces BIM into architectural design model and adopts Revit rapid modeling.It focuses on realizing rapid combination modeling of similar components through secondary ...Combining with engineering examples,this paper introduces BIM into architectural design model and adopts Revit rapid modeling.It focuses on realizing rapid combination modeling of similar components through secondary development,improving information conversion efficiency between engineering CAD and Revit building structure modeling,and quickly generating BIM structure construction drawings.展开更多
In the design process of globoidal CAM mechanism,there are some shortcomings such as long structure design cycle,low efficiency,and no modification flexibility of parameters of similar parts.NX Open classic applicatio...In the design process of globoidal CAM mechanism,there are some shortcomings such as long structure design cycle,low efficiency,and no modification flexibility of parameters of similar parts.NX Open classic application programming interfaces(APIS)can be connected with Microsoft Visual Studio(VS).Based on NX Open application,users can develop menus,toolbars,dialog boxes and other tools to realize interactive design.Users can also call input parameters through the dialog box callback function,then activate the corresponding control application(callback function)by C++programming language and NX Open API complete code.Based on the secondary development function of UG software and VS,a digital design system suitable for high efficiency modeling of globoidal indexing CAM mechanism was developed to complete the human-machine interface interaction design.The results show that comparing the digital design system with the traditional design,the system after parametric design can not only greatly shorten the design time,but also reduce the tedious repetitive work,which proves the superiority and feasibility of the digital design.展开更多
Secondary xylem development has long been recognized as a typical case of programmed cell death （PCD） in plants. During PCD, the degradation of genomic DNA is catalyzed by endonucleases. However, to date, no endonuc...Secondary xylem development has long been recognized as a typical case of programmed cell death （PCD） in plants. During PCD, the degradation of genomic DNA is catalyzed by endonucleases. However, to date, no endonuclease has been shown to participate in secondary xylem development. Two novel Ca^2＋-dependent DNase genes, EuCaN1 and EuCaN2, were identified from the differentiating secondary xylem of the tree Eucommia ulmoides Oliv., their functions were studied by DNase activity assay, in situ hybridization, protein immunolocalization and virus-induced gene silencing experiments. Full-length cDNAs of EuCaN1 and EuCaN2 contained an open reading frame of 987 bp, encoding two proteins of 328 amino acids with SNase-like functional domains. The genomic DNA sequence for EuCaN1 had no introns, while EuCaN2 had 8 introns. EuCaN1 and EuCaN2 digested ssDNA and dsDNA with Ca^2＋-dependence at neutral pH. Their expression was confined to differentiating secondary xylem cells and the proteins were localized in the nucleus. Their activity dynamics was closely correlated with secondary xylem development. Secondary xylem cell differentiation is influenced by RNAi of endonuclease genes. The results provide evidence that the Ca^2＋-dependent DNases are involved in secondary xylem development.展开更多
The COP9 signalosome(CSN) is a highly conserved multiprotein complex in all eukaryotes and involved in regulation of organism development. In filamentous fungi, several lines of evidence indicate that fungal developme...The COP9 signalosome(CSN) is a highly conserved multiprotein complex in all eukaryotes and involved in regulation of organism development. In filamentous fungi, several lines of evidence indicate that fungal development and secondary metabolism(SM) are mediated by the fifth subunit of CSN, called CsnE. Here we uncover a connection with CsnE and conidial formation as well as SM regulation in the plant endophytic fungus Pestalotiopsis fici. A homology search of the P. fici genome with CsnE, involved in sexual development and SM in Aspergillus nidulans, identified PfCsnE. Deletion of PfcsnE resulted in a mutant that stopped conidial production, but the conidia are recovered in a PfcsnE complemented strain. This indicates that PfCsnE is required for the formation of conidia. Secondary metabolite analysis demonstrated that the ΔPfcsnE strain produced more chloroisosulochrin, less ficiolide A production in comparison to wild type(WT). Transcriptome analysis of WT andΔPfcsnE strains indicated that PfcsnE impacts the expression levels of 8.37% of 14,797 annotated genes. Specifically, nine biosynthetic gene clusters(BGCs) were up-regulated and three BGCs were down-regulated by PfCsnE. Our results suggest that PfCsnE plays major roles in SM regulation and conidial development in P. fici.展开更多
I MIIT published'Standard Conditions for Secondary Lead Industry(Draft for soliciting opinions)'.Industry insiders pointed out that,based on the draft for soliciting opinions,MIIT will guide green development ...I MIIT published'Standard Conditions for Secondary Lead Industry(Draft for soliciting opinions)'.Industry insiders pointed out that,based on the draft for soliciting opinions,MIIT will guide green development of the industry,leading enterprises will receive support in capital and policy aspects.展开更多
OpenSees is a well-recognized open source platform with high compatibility,and it has a well-developed fiber element method to cope with nonlinear structural analysis.Fiber reinforced polymer(FRP)confined concrete can...OpenSees is a well-recognized open source platform with high compatibility,and it has a well-developed fiber element method to cope with nonlinear structural analysis.Fiber reinforced polymer(FRP)confined concrete can effectively improve the seismic performance of concrete structures.However,sophisticated constitutive models for FRP confined concrete are not available in the current version of OpenSees.In this paper,after reviewing several typical FRP confined concrete constitutive models,a modified constitutive model for FRP confined concrete in circular sections was proposed based on Lam and Teng(2003)’s model with four main modifications including the determination of FRP rupture strain,ultimate condition,envelope shape,and hysteretic rules.To embed the proposed constitutive model into OpenSees is a practical solution for engineering simulation.Hence,the secondary development of OpenSees New UserMat was briefly demonstrated and a set of critical steps were depicted in a flow chart.Finally,with the numerical implementations of a series of FRP confined concrete members covering a wide range of load cases,FRP confinement types and geometric properties,the utility and accuracy of the proposed model compared with Lam and Teng(2003)’s model and new material secondary development in OpenSees were well validated.展开更多
基金supported by a grant(No.14DZ2292800,http://www.greengeo.net/)from“Technology Service Platform of Civil Engineering”of Science and Technology Commission of Shanghai Municipality.
文摘Underground pipeline networks constitute a major component of urban infrastructure,and thus,it is imperative to have an efficient mechanism to manage them.This study introduces a secondary development system to efficiently model underground pipeline networks,using the building information modeling(BIM)-based software Revit.The system comprises separate pipe point and tubulation models.Using a Revit application programming interface(API),the spatial position and attribute data of the pipe points are extracted from a pipeline database,and the corresponding tubulation data are extracted from a tubulation database.Using the Family class in Revit API,the cluster in the self-built library of pipe point is inserted into the spatial location and the attribute data is added;in the same way,all pipeline instances in the pipeline system are created.The extension and localization of the model accelerated the modeling speed.The system was then used in a real construction project.The expansion of the model database and rapid modeling made the application of BIM technology in three-dimensional visualization of underground pipeline networks more convenient.Furthermore,it has applications in pipeline engineering construction and management.
基金Supported by National Natural Science Foundation of China(No.51109160)National High Technology Research and Development Program of China("863"Program,No.2012AA051705)+1 种基金International Science and Technology Cooperation Program of China(No.2012DFA70490)Tianjin Natural Science Foundation(No.13JCQNJC06900)
文摘The platform with bucket foundations can penetrate and migrate by underpressure/positive pressure technique caused by pumping water out/in the bucket. However, the construction process of bucket foundations cannot be clearly observed and effectively controlled due to the special nature of sea environment. By using an advanced simulation development tool of Multigen Creator, the visual construction simulation program for the platform with bucket foundations was developed to set up the virtual reality system with interaction control and observation in every view angle based on the secondary development technology of Vega platform. The application results show that the method is feasible and effective by simulating the whole construction process for the platform with four bucket foundations.
基金Supported by Special Topic of the Ministry of Education about Humanities and Social Sciences of China(No.12JDGC007)+2 种基金National Science and Technology Support Project of China(No.2011BAF09B01)International Scientific and Technological Cooperation Projects of China(No.2013DFB70350)
文摘This paper develops a strong secondary development based on ADAMS feature which creates high-speed rail bearings for simulation analysis module. This thesis is in the case of non-circular pattern instructions of how to achieve rapid roller modeling, with analysis of functions and parameters required for the design of the simulation module of the high-speed rail bearing, as well as the design of dialog boxes, the environment and file structure. The specific modules is based on the secondary development language provided by ADAMS ! View. Through the menus, dialog boxes which input parameters, it can achieve high iron bearing automatic modeling, dynamic analysis and post-processing to simplify the analysis of high-speed rail bearing operations, as well as improving the high-speed rail bearing development efficiency.
文摘In order to shorten the design cycle of the excavator working device, we have proposed a rapid modeling method for the excavator working device which uses parameters. Based on the Pro/toolkit, which is secondary development tool of Pro/E4.0,and combined with Vs C＋＋2005 programming software. It developed a flexible set of MFC visualization-friendly interfaces. Users can enter data in the visual interface according to their needs and it will generate a new part model quickly. So it improves the design quality, shortens the design cycle, and makes the cost lower significantly.
基金National Science and Technology Major Project,Grant/Award Number:2017YFC1503102National Natural Science Foundation of China,Grant/Award Number:51704144。
文摘The creep characteristics of rock under high crustal stress are of important influence on the long‐term stability of deep rock engineering.To study the creep characteristics and engineering application of sandstone under high crustal stress,this study constructed nonlinear creep damage(NCD)constitutive mode based on the triaxial graded loading‒unloading creep test of sandstone in the Yuezhishan Tunnel.A numerical NCD constitutive model and a breakable lining(BL)model were developed based on FLAC3D and then applied to the stability analysis of the Yuezhishan Tunnel.Based on the creep test results of sandstone,a power function of creep rate and stress level was constructed,by which the long‐term strength was solved.The results show that the long‐term strength of the red sandstone based on the related function of the steady‐state creep rate and stress level is close to the measured stress value in engineering.The NCD model considering damage factors reflects the instantaneous and viscoelastic plasticity deformation characteristics of the red sandstone.The numerical NCD constitutive model and the BL model can reflect surrounding rock deformation characteristics and lining failure characteristics in practical engineering.The research results provide theoretical references for long‐term stability analysis of rock engineering and the deformation control of surrounding rock under high crustal stress.
基金Under the auspices of the National Natural Science Foundation of China (No. 40161004, 40361002)Guangxi Natural Science Foundation (No. 023646, 0342001-2).
文摘In order to study the spatial-temporal change and environmental management of regional karst LUCC (land use and land cover change) and its causative environmental effect-rocky desertification by integrating qualitative analysis and quantitative analysis, and relying on RS, GIS and GPS (3S) techniques, karst land rocky derification dynamic monitoring and visualization management information system (KLRD.DMVM.IS) is framed, which includes design aim and structure model, function design, database design and model system design. The model system design gives priority to dynamic monitoring, drive force diagnosis, comprehensive evaluation and decision support of karst rocky desertification. From the viewpoint of model type, mathematic expression and its meaning, the dynamic monitoring models are concretely devised to reflect the spatial and temporal changing features and the trend of karst LUCC and rocky desertification. Taking Du'an Yao Autonomic County of Guangxi as an example, the KLRD.DMVM.IS is systematically analyzed in the application of the process and trend of karst LUCC and rocky desertification in Du'an County, and it provides the technical support for the study on karst land rocky desertification.
基金Supported by the Natural Sciences and Engineering Research Council of Canada, Discovery Grant to LS
文摘Although poplar is widely used for genomic and biotechnological manipulations of wood, the cellular basis of wood development in poplar has not been accurately documented at an ultrastructural level. Developing secondary xylem cells from hybrid poplar （Populus deltoides x P. trichocarpa）, which were actively making secondary cell walls, were preserved with high pressure freezing/freeze substitution for light and electron microscopy. The distribution of xylans and mannans in the different cell types of developing secondary xylem were detected with immunofluorescence and immuno-gold labeling. While xylans, detected with the monoclonal antibody LM10, had a general distribution across the secondary xylem, mannans were enriched in the S2 secondary cell wall layer of fibers. To observe the cellular structures associated with secondary wall production, cryofixed fibers were examined with transmission electron microscopy during differentiation. There were abundant cortical microtubules and endomembrane activity in cells during the intense phase of secondary cell wall synthesis. Microtubuleassociated small membrane compartments were commonly observed, as well as Golgi and secretory vesicles fusing with the plasma membrane.
基金supported by the Science and Technology Innovation Foundation of Hohai University(No. 2013-406096)
文摘Interval analysis is a new uncertainty analysis method for engineering structures. In this paper, a new sensitivity analysis method is presented by introducing interval analysis which can expand applications of the interval analysis method. The interval analysis process of sensitivity factor matrix of soil parameters is given. A method of parameter intervals and decision-making target intervals is given according to the interval analysis method. With FEM, secondary developments are done for Marc and the Duncan-Chang nonlinear elastic model. Mutual transfer between FORTRAN and Marc is implemented. With practial examples, rationality and feasibility are validated. Comparison is made with some published results.
基金Supported by National Natural Science Foundation of China (50574021)
文摘This paper focuses on the problem of low efficiency and limited non-geometric information handling ability in the process of 2D heating furnace design, proposes a 3D concurrent parametric design system, this method is realized by parametric design technology, which is supported by ActiveX-Automation technology and VBA technology, mutual visit between application programs makes batched modeling become possible, key dimensions are linked with each other by restrictions, so parts can be built concurrently by sharing few parameters between common borders, designer can be free from repeated drawing work during modification, Solid Edge is chosen as the modeling server, a secondary development software is programmed by Visual Basic, this system provides a feasible way to overlap time between different sections, the design efficiency and quality is improved significantly.
基金This work was supported by the National Natural Science Foundation of China(32071726 and 32271825).
文摘Secondary vascular development is a key biological characteristic of woody plants and the basis of wood formation.Our understanding of gene expression regulation and dynamic changes in microRNAs(miRNAs)during secondary vascular development is still limited.Here we present an integrated analysis of the miRNA and mRNA transcriptome of six phase-specific tissues-the shoot apex,procambium,primary vascular tissue,cambium,secondary phloem,and secondary xylem-in Populus tomentosa.Several novel regulatory modules,including the PtoTCP20-miR396d-PtoGRF15 module,were identified during secondary vascular development in Populus.A series of biochemical and molecular experiments confirmed that PtoTCP20 activated transcription of the miR396d precursor gene and that miR396d targeted PtoGRF15 to downregulate its expression.Plants overexpressing miR396d(35S:miR396d)showed enhanced secondary growth and increased xylem production.Conversely,during the transition from primary to secondary vascular development,plants with downregulated PtoTCP20expression(PtoTCP20-SRDX),downregulated miR396 expression(35S:STTM396),and PtoGRF15 overexpression(35S:PtoGRF15)showed delayed secondary growth.Novel regulatory modules were identified by integrated analysis of the miRNA and mRNA transcriptome,and the regulatory role of the PtoTCP20-miR396d-PtoGRF15 signaling cascade in secondary vascular development was validated in Populus,providing information to support improvements in forest cultivation and wood properties.
基金supported by the National Natural Science Foundation of China(Nos.11072143 and11272200)
文摘The materials with different moduli in tension and compression are called bi-modulus materials. Graphene is such a kind of materials with the highest strength and the thinnest thickness. In this paper, the mechanical response of the bi-modulus beam subjected to the temperature effect and placed on the Winkler foundation is studied. The differential equations about the neutral axis position and undetermined parameters of the normal strain of the bi-modulus foundation beam are established. Then, the analytical expressions of the normal stress, bending moment, and displacement of the foundation beam are derived. Simultaneously, a calculation procedure based on the finite element method （FEM） is developed to obtain the temperature stress of the bi-modulus struc- tures. It is shown that the obtained bi-modulus solutions can recover the classical modulus solution, and the results obtained by the analytical expressions, the present FEM proce- dure, and the traditional FEM software are consistent, which verifies the accuracy and reliability of the present analytical model and procedure. Finally, the difference between the bi-modulus results and the classical same modulus results is discussed, and several reasonable suggestions for calculating and optimizing the certain bi-modulus member in practical engineering are presented.
文摘GC-GIS system is a geochemical data processing system based on fractal theory. The system realized quantity statistics function by calling Surfer and MapInfo software, and it is compiled with Visual Basic language. This system is designed to integrate the functions both quantity statistics of Surfer and spatial data management of MapInfo. A new algorithm of fractal is added up to GC-GIS. Taking example for Weichang region of Hebei to test the system, the processing results show that the model can match the real distribution of mine well.
文摘Combining with engineering examples,this paper introduces BIM into architectural design model and adopts Revit rapid modeling.It focuses on realizing rapid combination modeling of similar components through secondary development,improving information conversion efficiency between engineering CAD and Revit building structure modeling,and quickly generating BIM structure construction drawings.
基金National Natural Science Foundation of China(51275210,51975251)Provi-ncial Industry-University-Research Joint Venture Project(1078081606192480)+1 种基金Jiangsu Key Laboratory of Advanced Food Manufacturing Equipment Technology(FMZ2018Y2)Six Talent Peaks(1076010241131350)。
文摘In the design process of globoidal CAM mechanism,there are some shortcomings such as long structure design cycle,low efficiency,and no modification flexibility of parameters of similar parts.NX Open classic application programming interfaces(APIS)can be connected with Microsoft Visual Studio(VS).Based on NX Open application,users can develop menus,toolbars,dialog boxes and other tools to realize interactive design.Users can also call input parameters through the dialog box callback function,then activate the corresponding control application(callback function)by C++programming language and NX Open API complete code.Based on the secondary development function of UG software and VS,a digital design system suitable for high efficiency modeling of globoidal indexing CAM mechanism was developed to complete the human-machine interface interaction design.The results show that comparing the digital design system with the traditional design,the system after parametric design can not only greatly shorten the design time,but also reduce the tedious repetitive work,which proves the superiority and feasibility of the digital design.
基金supported by the National Basic Research Program of China (2012CB114500)the National Natural Science Foundation of China (31070156)
文摘Secondary xylem development has long been recognized as a typical case of programmed cell death （PCD） in plants. During PCD, the degradation of genomic DNA is catalyzed by endonucleases. However, to date, no endonuclease has been shown to participate in secondary xylem development. Two novel Ca^2＋-dependent DNase genes, EuCaN1 and EuCaN2, were identified from the differentiating secondary xylem of the tree Eucommia ulmoides Oliv., their functions were studied by DNase activity assay, in situ hybridization, protein immunolocalization and virus-induced gene silencing experiments. Full-length cDNAs of EuCaN1 and EuCaN2 contained an open reading frame of 987 bp, encoding two proteins of 328 amino acids with SNase-like functional domains. The genomic DNA sequence for EuCaN1 had no introns, while EuCaN2 had 8 introns. EuCaN1 and EuCaN2 digested ssDNA and dsDNA with Ca^2＋-dependence at neutral pH. Their expression was confined to differentiating secondary xylem cells and the proteins were localized in the nucleus. Their activity dynamics was closely correlated with secondary xylem development. Secondary xylem cell differentiation is influenced by RNAi of endonuclease genes. The results provide evidence that the Ca^2＋-dependent DNases are involved in secondary xylem development.
基金Wenbing Yin is a scholar of "the 100 Talents Project" of Chinese Academy of Sciencessupported by the National Key Research and Development Program (2016YFD0400105)+1 种基金National Natural Science Foundation of China (31670402 and 31400334)Sate Key Laboratory of Mycology Open Project (SKLMKF 2015-1)
文摘The COP9 signalosome(CSN) is a highly conserved multiprotein complex in all eukaryotes and involved in regulation of organism development. In filamentous fungi, several lines of evidence indicate that fungal development and secondary metabolism(SM) are mediated by the fifth subunit of CSN, called CsnE. Here we uncover a connection with CsnE and conidial formation as well as SM regulation in the plant endophytic fungus Pestalotiopsis fici. A homology search of the P. fici genome with CsnE, involved in sexual development and SM in Aspergillus nidulans, identified PfCsnE. Deletion of PfcsnE resulted in a mutant that stopped conidial production, but the conidia are recovered in a PfcsnE complemented strain. This indicates that PfCsnE is required for the formation of conidia. Secondary metabolite analysis demonstrated that the ΔPfcsnE strain produced more chloroisosulochrin, less ficiolide A production in comparison to wild type(WT). Transcriptome analysis of WT andΔPfcsnE strains indicated that PfcsnE impacts the expression levels of 8.37% of 14,797 annotated genes. Specifically, nine biosynthetic gene clusters(BGCs) were up-regulated and three BGCs were down-regulated by PfCsnE. Our results suggest that PfCsnE plays major roles in SM regulation and conidial development in P. fici.
文摘I MIIT published'Standard Conditions for Secondary Lead Industry(Draft for soliciting opinions)'.Industry insiders pointed out that,based on the draft for soliciting opinions,MIIT will guide green development of the industry,leading enterprises will receive support in capital and policy aspects.
基金Project supported by the National Natural Science Foundation of China(Nos.51378292,51038006,and U1134110)the China Postdoctoral Science Foundation(No.2013M530048)
文摘OpenSees is a well-recognized open source platform with high compatibility,and it has a well-developed fiber element method to cope with nonlinear structural analysis.Fiber reinforced polymer(FRP)confined concrete can effectively improve the seismic performance of concrete structures.However,sophisticated constitutive models for FRP confined concrete are not available in the current version of OpenSees.In this paper,after reviewing several typical FRP confined concrete constitutive models,a modified constitutive model for FRP confined concrete in circular sections was proposed based on Lam and Teng(2003)’s model with four main modifications including the determination of FRP rupture strain,ultimate condition,envelope shape,and hysteretic rules.To embed the proposed constitutive model into OpenSees is a practical solution for engineering simulation.Hence,the secondary development of OpenSees New UserMat was briefly demonstrated and a set of critical steps were depicted in a flow chart.Finally,with the numerical implementations of a series of FRP confined concrete members covering a wide range of load cases,FRP confinement types and geometric properties,the utility and accuracy of the proposed model compared with Lam and Teng(2003)’s model and new material secondary development in OpenSees were well validated.
