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The Advantages of Methane Production by Combined Fermentation of Lignite and Wheat Straw
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作者 Jiayuan Gu 《Advances in Bioscience and Biotechnology》 CAS 2024年第1期1-14,共14页
Biogasification of coal is important for clean utilization of coal. Experiments on the fermentation of single lignite, single straw and their mixture were performed to explore the variation characteristics of gas prod... Biogasification of coal is important for clean utilization of coal. Experiments on the fermentation of single lignite, single straw and their mixture were performed to explore the variation characteristics of gas production potential, microbial community and methanogenic metabolic pathways of mixture. Research has shown that mixed fermentation of lignite and straw significantly promoted biomethane production. The abundance of hydrolytic acidifying functional bacteria genera (Sphaerochaeta, Lentimicrobium) in mixed fermentation was higher than that in the fermentation of single lignite and single straw. The abundance of some key CAZy metabolic enzyme gene sequences in mixed fermentation group was increased, which was favorable to improve methane production. Aceticlastic methanogenesis was the most critical methanogenic pathway and acetic acid pathway was more competitive in methanogenic mode during peak fermentation. Macrogenomics provided theoretical support for the claim that mixed fermentation of coal and straw promoted biomethane metabolism, which was potentially valuable in expanding methanogenesis from mixed fermentation of lignite with different biomasses. 展开更多
关键词 LIGNITE Wheat Straw Mixed fermentation Microbial Community Macrogenomics
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Influence of nitrogen status on fermentation performances of non-Saccharomyces yeasts:a review
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作者 Jinchen Li Mengmeng Yuan +3 位作者 Nan Meng Hehe Li Jinyuan Sun Baoguo Sun 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期556-567,共12页
Nitrogen,one of the most crucial nutrients present in grapes and musts,plays a key role in yeast activities during alcoholic fermentation.Such influences are imposed on yeast growth and fermentation performances inclu... Nitrogen,one of the most crucial nutrients present in grapes and musts,plays a key role in yeast activities during alcoholic fermentation.Such influences are imposed on yeast growth and fermentation performances including the formation of secondary metabolites.Saccharomyces cerevisiae,the main yeast responsible for fermentation,has been studied extensively regarding nitrogen impacts.On the other hand,a similar study for non-Saccharomyces yeasts,whose contributions to winemaking have gradually been acknowledged,remains to be fully explored,with a few studies being reported.This review starts by discussing nitrogen impacts on non-Saccharomyces yeast growth and fermentation kinetics in different case scenarios,then proceeds to summarize the nitrogen preferences of individual yeast strains with regulation mechanisms elucidated by recent studies.Detailed discussions on the influences on the production of volatile compounds and proposed pathways therein are made,followed by future work suggested as the final section.In summarizing the nitrogen impacts on non-Saccharomyces yeasts throughout alcoholic fermentation,this review will be helpful in obtaining a more comprehensive view on these non-conventional wine yeasts in terms of nutrient requirements and corresponding volatile production.Research gaps will therefore be elucidated for future research. 展开更多
关键词 Non-Saccharomyces yeasts NITROGEN fermentation kinetics Nitrogen preference Wine aroma
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Secondary Metabolites of Entomopathogenic Fungi, Biological Alternative for the Control of Agricultural Pests and Disease: Present and Perspectives
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作者 Nallely San Juan-Maldonado Silvia Rodríguez-Navarro +2 位作者 Alejandro Angel-Cuapio José Norberto Vásquez Bonilla Juan Esteban Barranco-Florido 《Advances in Enzyme Research》 CAS 2024年第1期1-15,共15页
The use of entomopathogenic fungi (EF) in recent years has been highly effective against the different orders of insects considered pests of agricultural importance and their conidia have been commonly applied, but it... The use of entomopathogenic fungi (EF) in recent years has been highly effective against the different orders of insects considered pests of agricultural importance and their conidia have been commonly applied, but it has been reported that these are sensitive to the environmental conditions. For this reason, biopesticides products have been formulated based on secondary metabolites, recently. These biomolecules participate as biological control agent, such as: cyclic depsipeptides, amino acids, polyketides, polyphenols and terpenoids, affecting their morphology, life cycle and insect behavior. The use of secondary metabolites of entomopathogenic fungi opens the possibility of application in a more efficient way for the control of agricultural pests in a compatible with the environment and human health;therefore, it is important to know, analyzing the type of molecules, their effects, and their different methods of application. 展开更多
关键词 secondary Metabolites Entomopathogenic Fungi Biological Control Agent
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Insights into microbiota community dynamics and flavor development mechanism during golden pomfret(Trachinotus ovatus)fermentation based on single-molecule real-time sequencing and molecular networking analysis
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作者 Yueqi Wang Qian Chen +5 位作者 Huan Xiang Dongxiao Sun-Waterhouse Shengjun Chen Yongqiang Zhao Laihao Li Yanyan Wu 《Food Science and Human Wellness》 SCIE CSCD 2024年第1期101-114,共14页
Popular fermented golden pomfret(Trachinotus ovatus)is prepared via spontaneous fermentation;however,the mechanisms underlying the regulation of its flavor development remain unclear.This study shows the roles of the ... Popular fermented golden pomfret(Trachinotus ovatus)is prepared via spontaneous fermentation;however,the mechanisms underlying the regulation of its flavor development remain unclear.This study shows the roles of the complex microbiota and the dynamic changes in microbial community and flavor compounds during fish fermentation.Single-molecule real-time sequencing and molecular networking analysis revealed the correlations among different microbial genera and the relationships between microbial taxa and volatile compounds.Mechanisms underlying flavor development were also elucidated via KEGG based functional annotations.Clostridium,Shewanella,and Staphylococcus were the dominant microbial genera.Forty-nine volatile compounds were detected in the fermented fish samples,with thirteen identified as characteristic volatile compounds(ROAV>1).Volatile profiles resulted from the interactions among the microorganisms and derived enzymes,with the main metabolic pathways being amino acid biosynthesis/metabolism,carbon metabolism,and glycolysis/gluconeogenesis.This study demonstrated the approaches for distinguishing key microbiota associated with volatile compounds and monitoring the industrial production of high-quality fermented fish products. 展开更多
关键词 Fermented golden pomfret Microbiota community Volatile compound Co-occurrence network Metabolic pathway
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A perspective review on the biosynthesis of plant-based secondary metabolites and their application as potent drugs
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作者 JHILAM PRAMANIK AKASH KUMAR +7 位作者 SARVESH RUSTAGI MEHAK KATYAL SHEETAL THAKUR JUTISHNA BORA SUMIRA MALIK ANCHAL TREHAN NAYAN TALUKDAR PETR SLAMA 《BIOCELL》 SCIE 2024年第4期541-557,共17页
Many phytochemicals and their derived metabolites produced by plants are extensively employed in commercial goods,pharmaceutical products as well as in the environmental and medicalfields.However,these secondary metabo... Many phytochemicals and their derived metabolites produced by plants are extensively employed in commercial goods,pharmaceutical products as well as in the environmental and medicalfields.However,these secondary metabolites obtained from plants are in low amounts,and it is difficult to synthesize them at the industrial level.Despite these challenges,they may be utilized for a variety of medicinal products that are either available in the market or are being researched and tested.Secondary metabolites are complex compounds that exhibit chirality.Further,under controlled conditions with elicitors,desired secondary metabolites may be produced from plant cell cultures.This review emphasizes the various aspects of secondary metabolites including their types,synthesis,and applications as medicinal products.The article aims to promote the use of plant secondary metabolites in the management and treatment of various diseases. 展开更多
关键词 secondary metabolites Medicinal plant Synthesis Health benefits
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Effects of Rosa roxburghii&edible fungus fermentation broth on immune response and gut microbiota in immunosuppressed mice
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作者 Dechang Xu Jielun Hu +4 位作者 Yadong Zhong Yanli Zhang Wenting Liu Shaoping Nie Mingyong Xie 《Food Science and Human Wellness》 SCIE CSCD 2024年第1期154-165,共12页
With the rise of probiotics fermentation in food industry,fermented foods have attracted worldwide attention.In this study,protective effects of Rosa roxburghii&edible fungus fermentation broth(REFB)on immune func... With the rise of probiotics fermentation in food industry,fermented foods have attracted worldwide attention.In this study,protective effects of Rosa roxburghii&edible fungus fermentation broth(REFB)on immune function and gut health in Cyclophosphamide induced immunosuppressed mice were investigated.Results showed that REFB could improve the immune organ index,and promote the proliferation and differentiation of splenic T lymphocytes.In addition,it attenuated intestinal mucosal damage and improved intestinal cellular immunity.REFB administration also up-regulated the expression of IL-4,INF-γ,TNF-α,T-bet and GATA-3 mRNA in small intestine.Furthermore,administration of REFB modulated gut microbiota composition and increased the relative abundance of beneficial genus,such as Bacteroides.It also increased the production of fecal short-chain fatty acids.These indicate that REFB has the potential to improve immunity,alleviate intestinal injury and regulate gut microbiota in immunosuppressed mice. 展开更多
关键词 Fermented foods Immunosuppressed mice Immune response Gut microbiota Short-chain fatty acids
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A spatiotemporal evolution model of a short-circuit arc to a secondary arc based on the improved charge simulation method
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作者 丛浩熹 王宇轩 +2 位作者 乔力盼 苏文晶 李庆民 《Plasma Science and Technology》 SCIE EI CAS CSCD 2024年第1期62-73,共12页
The initial shape of the secondary arc considerably influences its subsequent shape.To establish the model for the arcing time of the secondary arc and modify the single-phase reclosing sequence,theoretical and experi... The initial shape of the secondary arc considerably influences its subsequent shape.To establish the model for the arcing time of the secondary arc and modify the single-phase reclosing sequence,theoretical and experimental analysis of the evolution process of the short-circuit arc to the secondary arc is critical.In this study,an improved charge simulation method was used to develop the internal-space electric-field model of the short-circuit arc.The intensity of the electric field was used as an independent variable to describe the initial shape of the secondary arc.A secondary arc evolution model was developed based on this model.Moreover,the accuracy of the model was evaluated by comparison with physical experimental results.When the secondary arc current increased,the arcing time and dispersion increased.There is an overall trend of increasing arc length with increasing arcing time.Nevertheless,there is a reduction in arc length during arc ignition due to short circuits between the arc columns.Furthermore,the arcing time decreased in the range of 0°-90°as the angle between the wind direction and the x-axis increased.This work investigated the method by which short-circuit arcs evolve into secondary arcs.The results can be used to develop the secondary arc evolution model and to provide both a technical and theoretical basis for secondary arc suppression. 展开更多
关键词 short-circuit arc secondary arc STOCHASTICITY improved charge simulation method arc time
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Highly divergent sympatric lineages of Leptotila verreauxi(Aves:Columbidae)suggest a secondary contact area in the Isthmus of Tehuantepec,Mexico
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作者 Orlando J.Espinosa-Chávez Adolfo G.Navarro-Sigüenza +1 位作者 Hernando Rodríguez-Correa Luis A.Sánchez-González 《Avian Research》 SCIE CSCD 2024年第1期12-21,共10页
Due to a complex geological and biotic history,the Isthmus of Tehuantepec(IT),has been long recognized as a driver for the evolutionary divergence of numerous lowland and highland taxa.Widely distributed in the lowlan... Due to a complex geological and biotic history,the Isthmus of Tehuantepec(IT),has been long recognized as a driver for the evolutionary divergence of numerous lowland and highland taxa.Widely distributed in the lowlands of the American continent,the White-Tipped Dove(Leptotila verreauxi)is a polytypic species with 13 recognized subspecies.Four of these have been recorded in Mexico,and the distribution of three abuts at the IT,suggesting a contact zone.To estimate phylogenetic patterns,divergence times and genetic differentiation,we examined two mt DNA(ND2 and COI)and one n DNA(β-fibint 7)markers.We also used correlative ecological niche models(ENM)to assess whether ecological differences across the IT may have acted as a biogeographical boundary.We estimated paleodistributions during the Middle Holocene,Last Glacial Maximum and Last Interglacial,to evaluate the influence of climate changes on the distribution and demographic changes.Our results showed genetically distinct lineages that diverged approximately 2.5 million years ago.Climatic and ecological factors may have played a dual role in promoting differentiation,but also in the formation of a secondary contact zone in the southern IT.Our ecological niche comparisons indicated that the ecological niche of sympatric lineages at the IT are not identical,suggesting niches divergence;in addition,environmental niche models across the region indicated no abrupt biogeographic barriers,but the presence of regions with low suitability.These results suggest that genetic differentiation originated by a vicariant event probably related to environmental factors,favored the evolution of different ecological niches.Also,the absence of a biogeographic barrier but the presence of less suitable areas in the contact regions,suggest that secondary contact zones may be also maintained by climatic factors for the eastern group,but also by biotic interactions for the western group. 展开更多
关键词 Biogeographical boundary Humid and dry forests Isthmus of Tehuantepec PHYLOGEOGRAPHY secondary contact
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Structure and immunomodulatory activity of Lentinus edodes polysaccharides modified by probiotic fermentation
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作者 Jingjing Liang Meina Zhang +6 位作者 Xiaohan Li Yuan Yue Xiaowei Wang Mengzhen Han Tianli Yue Zhouli Wang Zhenpeng Gao 《Food Science and Human Wellness》 SCIE CSCD 2024年第1期421-433,共13页
Plant-based fermentations provide an untapped source for novel biotechnological applications.In this study,a probiotic named Lactobacillus fermentum 21828 was introduced to ferment Lentinus edodes.Polysaccharides were... Plant-based fermentations provide an untapped source for novel biotechnological applications.In this study,a probiotic named Lactobacillus fermentum 21828 was introduced to ferment Lentinus edodes.Polysaccharides were extracted from fermented and non-fermented L.edodes and purified via DEAE-52 and Sephadex G-100.The components designated F-LEP-2a and NF-LEP-2a were analyzed by FT-IR,HPGPC,HPAEC,SEM,GC-MS and NMR.The results revealed that probiotic fermentation increased the molecular weight from 1.16×10^(4) Da to 1.87×10^(4) Da and altered the proportions of glucose,galactose and mannose,in which glucose increased from 45.94%to 48.16%.Methylation analysis and NMR spectra indicated that F-LEP-2a and NF-LEP-2a had similar linkage patterns.Furthermore,their immunomodulatory activities were evaluated with immunosuppressive mice.NF-LEP and F-LEP improved immune organ indices,immunoglobulin(Ig G and Ig M)and cytokines concentrations;restored the antioxidation capacity of liver;and maintained the balance of gut microbiota.F-LEP displayed better moderating effects on the spleen index,immunoglobulin,cytokines and the diversity of gut microbiota than NF-LEP(200,400 mg/kg).Our study provides an efficient and environment-friendly way for the structural modification of polysaccharides,which helps to enhance their biological activity and promote their wide application in food,medicine and other fields. 展开更多
关键词 Lentinus edodes polysaccharide Lactobacillus fermentum fermentation Structural analysis Immunoregulatory activity Nuclear magnetic resonance
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Allergen degradation of bee pollen by lactic acid bacteria fermentation and its alleviatory effects on allergic reactions in BALB/c mice
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作者 Shuting Yin Qiangqiang Li +5 位作者 Yuxiao Tao Enning Zhou Kun Wang Wanwen Chen Xiangxin Li Liming Wu 《Food Science and Human Wellness》 SCIE CSCD 2024年第1期349-359,共11页
Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lac... Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lactic acid bacteria(LAB)were used to ferment Brassica napus BP for alleviating its allergenicity.Four novel allergens(glutaredoxin,oleosin-B2,catalase and lipase)were identified with significant decreases in LAB-fermented BP(FBP)than natural BP by proteomics.Meanwhile,metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP,indicating the degradation of LAB on allergens.Moreover,FBP showed alleviatory effects in BALB/c mice,which relieved pathological symptoms and lowered production of allergic mediators.Microbial high-throughput sequencing analysis showed that FBP could regulate gut microbiota and metabolism to strengthen immunity,which were closely correlated with the alleviation of allergic reactivity.These findings could contribute to the development and utilization of hypoallergenic BP products. 展开更多
关键词 Alleviatory effects Lactic acid bacteria fermentation Bee pollen allergen Gut microbiota Host metabolism
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Study on Solid Fermentation and Antioxidant Function of Natto
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作者 Junxia SONG Hongbing QI Yanhong MAO 《Asian Agricultural Research》 2024年第1期32-36,共5页
[Objectives]To study the optimum conditions of solid fermentation of natto with antioxidant function as an index.[Methods]Single factor experiment and orthogonal experiment were designed to study the effects of temper... [Objectives]To study the optimum conditions of solid fermentation of natto with antioxidant function as an index.[Methods]Single factor experiment and orthogonal experiment were designed to study the effects of temperature,time,initial pH and inoculum amount on the antioxidant activity of natto solid fermentation.The optimum conditions of natto solid fermentation were determined and the antioxidant ac-tivity of natto extract was compared.[Results]The optimal fermentation conditions were as follows:temperature 32℃,initial pH 7.0,inocu-lation amount 8%,fermentation time 32 h.The hydroxyl radical scavenging rate of natto solid fermentation crude extract was the highest,which was 82.7%.The optimized nato fermentation extract showed stronger scavenging ability for-OH and O,:,and showed obvious dose-effect relationship.ICso was 3.63 and 4.24 mg/mL,respectively,and the scavenging efficiency was 1.3 and 1.9 times higher than that of the unoptimized fermentation extract,respectively.[Conclusions]Natto is rich in nattokinase and other functional factors,and its antioxidant ac-tivity can be improved by optimizing fermentation technology,so that natto products can be widely used,including cosmetic raw materials,nat-to skin care soap,health food and medicine,etc.,and have a broader development prospect. 展开更多
关键词 NATTO Solid fermentation Hydroxyl radical scavenging rate Antioxidant activity
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Optimal Cooperative Secondary Control for Islanded DC Microgrids via a Fully Actuated Approach
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作者 Yi Yu Guo-Ping Liu +1 位作者 Yi Huang Peng Shi 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2024年第2期405-417,共13页
DC-DC converter-based multi-bus DC microgrids(MGs) in series have received much attention, where the conflict between voltage recovery and current balancing has been a hot topic. The lack of models that accurately por... DC-DC converter-based multi-bus DC microgrids(MGs) in series have received much attention, where the conflict between voltage recovery and current balancing has been a hot topic. The lack of models that accurately portray the electrical characteristics of actual MGs while is controller design-friendly has kept the issue active. To this end, this paper establishes a large-signal model containing the comprehensive dynamical behavior of the DC MGs based on the theory of high-order fully actuated systems, and proposes distributed optimal control based on this. The proposed secondary control method can achieve the two goals of voltage recovery and current sharing for multi-bus DC MGs. Additionally, the simple structure of the proposed approach is similar to one based on droop control, which allows this control technique to be easily implemented in a variety of modern microgrids with different configurations. In contrast to existing studies, the process of controller design in this paper is closely tied to the actual dynamics of the MGs. It is a prominent feature that enables engineers to customize the performance metrics of the system. In addition, the analysis of the stability of the closed-loop DC microgrid system, as well as the optimality and consensus of current sharing are given. Finally, a scaled-down solar and battery-based microgrid prototype with maximum power point tracking controller is developed in the laboratory to experimentally test the efficacy of the proposed control method. 展开更多
关键词 DC microgrids distributed control high-order fully actuated system approach linear quadratic regulator microgrid modeling secondary control
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Investigation of the Fermentation Mode of Rice Husk for the Stabilization of Earth Plaster
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作者 Nafissatou Savadogo Yasmine Binta Traore +2 位作者 Nathael Lankoande Philbert Nshimiyimana Adamah Messan 《Journal of Minerals and Materials Characterization and Engineering》 2024年第1期17-36,共20页
Despite its low resistance to humidity, adobe remains the most widely used material for housing construction, particularly in developing countries. The present study aims to assess different modes of use of fermented ... Despite its low resistance to humidity, adobe remains the most widely used material for housing construction, particularly in developing countries. The present study aims to assess different modes of use of fermented RH and to evaluate their influence on the behavior of raw earth for application in plaster. The influences of two types of RH are evaluated: granular rice husk (RHg) and powdered RH (RHp). The clay mainly consists of clay (40%), silt (22%), and sand (38.4%), with a small proportion of gravel (0.24%). Its liquidity limit is 40% and the plasticity index is 26.5%. The mixtures were designed using earth and each of the two rice husks at the volumetric content of 10%, 15% and 20% of the total volume mixed with water 36.5%, 38.5% and 40.3% and fermented for three weeks. Each fermented mixture was added to the soil to form the paste, and 40 × 40 × 160 mm<sup>3</sup> test speciments were made for characterization. The results generally show an improvement in the physico-mechanical properties and water resistance of the mortars containing fermented RH, with an optimal content between 10% and 15%. The powdered RH improved the performance of the mortar better than granular RH. 展开更多
关键词 Earth Plaster Rice Husk CLAY fermentation ADOBE
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Commuting Space of Primary and Secondary Schools in the Context of Child Friendly Cities:A Case Study of Bajiao Street of Shijingshan District
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作者 LIU Ping PENG Li +2 位作者 WANG Zhihao CONG Jing DING Yuqi 《Journal of Landscape Research》 2024年第1期22-26,30,共6页
With the continuous promotion of the construction of child friendly cities,the school commuting space is an important component of the construction of child friendly roads.Based on the background of child friendly cit... With the continuous promotion of the construction of child friendly cities,the school commuting space is an important component of the construction of child friendly roads.Based on the background of child friendly cities,the commuting space of 11 primary and secondary schools in Bajiao Street is analyzed through literature analysis and field research methods.Firstly,the relevant literature on school commuting space is sorted out,and the characteristics of school commuting space are summarized,including transportation,landscape,culture,leisure,and security.Secondly,the characteristics of commuting space of primary and secondary schools in Bajiao Street are analyzed from three aspects:in front of the school gate,path space,and node space.This paper aims to provide reference and guidance for the future construction of children’s walking school commuting and promote the construction of a child friendly city. 展开更多
关键词 Child friendly cities School commuting space Bajiao Street Primary and secondary schools
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Analysis of the Design and Application of a Novel CT Secondary Cable Line Calibrator
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作者 Jiaxing Ren 《Journal of Electronic Research and Application》 2024年第2期7-11,共5页
With the rapid development of electrical power systems,ensuring the accuracy and reliability of power transmis-sion has become particularly crucial.The secondary cable line calibrator for current transformers(CT)plays... With the rapid development of electrical power systems,ensuring the accuracy and reliability of power transmis-sion has become particularly crucial.The secondary cable line calibrator for current transformers(CT)plays an essential role in calibrating electrical power systems.It is not only related to the safe operation of the system but also directly im-pacts the accuracy of energy metering.This study aims to design and analyze an efficient CT secondary cable line calibra-tor to explore its application effects in the power system.By thoroughly analyzing the characteristics of CT secondary ca-ble lines and the design requirements of the calibrator,this paper proposes an innovative design scheme for the calibrator.This device demonstrates significant effects in enhancing the accuracy and stability of power system calibration,providing robust technical support for the optimization and upgrade of the power system.This research not only offers a theoretical basis and practical guidance for the design and application of CT secondary cable line calibrators but also contributes new ideas and methods for the precise calibration and efficient management of the power system. 展开更多
关键词 CT secondary cable line CALIBRATOR Power system DESIGN Application
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Effect of cooked rice with added fructo-oligosaccharide on faecal microorganisms investigated by in vitro digestion and fermentation 被引量:2
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作者 Fei Pei Wen Li +5 位作者 Xiaolei Ni Xinyang Sun Yijun Yao Yong Fang Wenjian Yang Qiuhui Hu 《Food Science and Human Wellness》 SCIE CSCD 2023年第2期662-668,共7页
In the present study,the effects of cooked rice(CR)with added fructo-oligosaccharide(FOS)on faecal flora were studied by a simulated in vitro digestion and fermentation method.The total carbohydrate content,p H,and s ... In the present study,the effects of cooked rice(CR)with added fructo-oligosaccharide(FOS)on faecal flora were studied by a simulated in vitro digestion and fermentation method.The total carbohydrate content,p H,and s hort-chain fatty acids(SCFAs)were determined during in vitro digestion and fermentation.The change in the bacterial phase distribution after the fermentation was also analysed.The results showed that t he total carbohydrate content of the CR with added FOS(FCR)significantly decreased during the simulated digestion.Meanwhile,the p H of the FCR decreased and the SCFAs concentration increased significantly compared to those of the CR during the simulated fermentation.In addition,the FCR showed the advantage of promoting beneficial bacteria,such as Bifidobacterium and Lactobacillus,and inhibiting harmful bacteria,such as Bacteroides and Klebsiella compared to the CR.Therefore,the FOS as a prebiotic could be recommended to produce the high-quality healthy rice food. 展开更多
关键词 FRUCTO-OLIGOSACCHARIDE In vitro fermentation Rice Short-chain fatty acids Bacterial phase distribution
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Melatonin promotes the development of the secondary hair follicles by regulating circMPP5 被引量:1
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作者 Xiaogao Diao Lingyun Yao +3 位作者 Tao Duan Jiaxin Qin Liwen He Wei Zhang 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第4期1696-1712,共17页
Background The quality and yield of cashmere fibre are closely related to the differentiation and development of secondary hair follicles in the skin of cashmere goats.The higher the density of secondary hair follicle... Background The quality and yield of cashmere fibre are closely related to the differentiation and development of secondary hair follicles in the skin of cashmere goats.The higher the density of secondary hair follicles,the higher the quality and yield of cashmere from the fleece.Development of secondary hair follicles commences in the embryonic stage of life and is completed 6 months after birth.Preliminary experimental results from our laboratory showed that melatonin(MT)treatment of goat kids after their birth could increase the density of secondary hair follicles and,thus,improve the subsequent yield and quality of cashmere.These changes in the secondary hair follicles resulted from increases in levels of antioxidant and expression of anti-apoptotic protein,and from a reduction in apoptosis.The present study was conducted to explore the molecular mechanism of MT-induced secondary hair follicle differentiation and development by using whole-genome analysis.Results MT had no adverse effect on the growth performance of cashmere kids but significantly improved the character of the secondary hair follicles and the quality of cashmere,and this dominant effect continued to the second year.Melatonin promotes the proliferation of secondary hair follicle cells at an early age.The formation of secondary hair follicles in the MT group was earlier than that in the control group in the second year.The genome-wide data results involved KEGG analysis of 1044 DEmRNAs,91 DElncRNAs,1054 DEcircRNAs,and 61 DEmiRNAs which revealed that the mitogen-activated protein kinase(MAPK)signaling pathway is involved in the development of secondary hair follicles,with key genes(FGF2,FGF21,FGFR3,MAPK3(ERK1))being up-regulated and expressed.We also found that the circMPP5 could sponged miR-211 and regulate the expression of MAPK3.Conclusions We conclude that MT achieves its effects by regulating the MAPK pathway through the circMPP5 sponged the miR-211,regulating the expression of MAPK3,to induce the differentiation and proliferation of secondary hair follicle cells.In addition there is up-regulation of expression of the anti-apoptotic protein causing reduced apoptosis of hair follicle cells.Collectively,these events increase the numbers of secondary hair follicles,thus improving the production of cashmere from these goats. 展开更多
关键词 CASHMERE MAPK MELATONIN miR-211 secondary hair follicle
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Artificial simulated saliva,gastric and intestinal digestion and fermentation in vitro by human gut microbiota of intrapolysaccharide from Paecilomyces cicadae TJJ1213 被引量:1
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作者 Juanjuan Tian Xiaomeng Wang +6 位作者 Xueliang Zhang Xiaohong Chen Mingsheng Dong Xin Rui Qiuqin Zhang Mei Jiang Wei Li 《Food Science and Human Wellness》 SCIE CSCD 2023年第2期622-633,共12页
In this study,the in vitro digestion and fermentation of two intra-polysaccharide fractions(IPS1 and IPS2)from Paecilomyces cicadae TJJ1213 were investigated.The constituent monosaccharides of IPS1 and IPS2 were not c... In this study,the in vitro digestion and fermentation of two intra-polysaccharide fractions(IPS1 and IPS2)from Paecilomyces cicadae TJJ1213 were investigated.The constituent monosaccharides of IPS1 and IPS2 were not changed after simulated saliva,gastric and small intestinal digestion.However,they can be hydrolyzed and utilized by gut microbiota,and short-chain fatty acids(SCFAs)level were increased after IPS1 and IPS2 treatments.Furthermore,16 S r RNA sequencing analysis of fermentation samples were performed.Alpha-diversity,beta-diversity and taxonomic composition differences analysis revealed that IPS1 and IPS2 promoted the proliferation of beneficial bacteria and modulated the overall structure of gut microbiota.Taxonomic comparison analysis found that IPS1 increased the relative abundances of beneficial bacteria including Megamonas,Bifidobacterium and Lactobacillus,while IPS2 could increase the abundance of Bacteroides,Parabacteroides and Phascolarctobacterium.In addition,they can also decrease the levels of pathogenic bacteria containing Escherichia-Shigella,Klebsiella and Fusobacterium.These results indicated that IPS from Paecilomyces cicadae TJJ1213 could be used as potential candidates for new functional foods. 展开更多
关键词 Paecilomyces cicadae TJJ1213 POLYSACCHARIDE fermentation Gut microbiota
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Effects of inoculating feruloyl esterase-producing Lactiplantibacillus plantarum A1 on ensiling characteristics,in vitro ruminal fermentation and microbiota of alfalfa silage 被引量:1
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作者 Fuhou Li Samaila Usman +6 位作者 Wenkang Huang Mengya Jia Zohreh Akhavan Kharazian Tao Ran Fadi Li Zitong Ding Xusheng Guo 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2023年第4期1713-1729,共17页
Background Ferulic acid esterase(FAE)-secreting Lactiplantibacillus plantarum A1(Lp A1)is a promising silage inoculant due to the FAE’s ability to alter the plant cell wall structure during ensiling,an action that is... Background Ferulic acid esterase(FAE)-secreting Lactiplantibacillus plantarum A1(Lp A1)is a promising silage inoculant due to the FAE’s ability to alter the plant cell wall structure during ensiling,an action that is expected to improve forage digestibility.However,little is known regarding the impacts of Lp A1 on rumen microbiota.Our research assessed the influences of Lp A1 in comparison to a widely adopted commercial inoculant Lp MTD/1 on alfalfa’s ensilage,in vitro rumen incubation and microbiota.Results Samples of fresh and ensiled alfalfa treated with(either Lp A1 or Lp MTD/1)or without additives(as control;CON)and ensiled for 30,60 and 90 d were used for fermentation quality,in vitro digestibility and batch culture study.Inoculants treated silage had lower(P<0.001)pH,acetic acid concentration and dry matter(DM)loss,but higher(P=0.001)lactic acid concentration than the CON during ensiling.Compared to the CON and Lp MTD/1,silage treated with Lp A1 had lower(P<0.001)aNDF,ADF,ADL,hemicellulose,and cellulose contents and higher(P<0.001)free ferulic acid concentration.Compared silage treated with Lp MTD/1,silage treated with Lp A1 had significantly(P<0.01)improved ruminal gas production and digestibility,which were equivalent to those of fresh alfalfa.Realtime PCR analysis indicated that Lp A1 inoculation improved the relative abundances of rumen’s total bacteria,fungi,Ruminococcus albus and Ruminococcus flavefaciens,while the relative abundance of methanogens was reduced by Lp MTD/1 compared with CON.Principal component analysis of rumen bacterial 16S rRNA gene amplicons showed a clear distinction between CON and inoculated treatments without noticeable distinction between Lp A1 and Lp MTD/1 treatments.Comparison analysis revealed differences in the relative abundance of some bacteria in different taxa between Lp A1 and Lp MTD/1 treatments.Silage treated with Lp A1 exhibited improved rumen fermentation characteristics due to the inoculant effects on the rumen microbial populations and bacterial community.Conclusions Our findings suggest that silage inoculation of the FAE-producing Lp A1 could be effective in improving silage quality and digestibility,and modulating the rumen fermentation to improve feed utilization. 展开更多
关键词 DIGESTIBILITY Ferulic acid esterase In vitro fermentation Rumen microbial community SILAGE
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High-resolution visible imaging with piezoelectric deformable secondary mirror: experimental results at the 1.8-m adaptive telescope 被引量:1
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作者 Youming Guo Kele Chen +7 位作者 Jiahui Zhou Zhengdai Li Wenyu Han Xuejun Rao Hua Bao Jinsheng Yang Xinlong Fan Changhui Rao 《Opto-Electronic Advances》 SCIE EI CAS CSCD 2023年第12期15-26,共12页
Integrating deformable mirrors within the optical train of an adaptive telescope was one of the major innovations in astronomical observation technology,distinguished by its high optical throughput,reduced optical sur... Integrating deformable mirrors within the optical train of an adaptive telescope was one of the major innovations in astronomical observation technology,distinguished by its high optical throughput,reduced optical surfaces,and the incorporation of the deformable mirror.Typically,voice-coil actuators are used,which require additional position sensors,internal control electronics,and cooling systems,leading to a very complex structure.Piezoelectric deformable secondary mirror technologies were proposed to overcome these problems.Recently,a high-order piezoelectric deformable secondary mirror has been developed and installed on the 1.8-m telescope at Lijiang Observatory in China to make it an adaptive telescope.The system consists of a 241-actuator piezoelectric deformable secondary mirror,a 192-sub-aperture Shack-Hartmann wavefront sensor,and a multi-core-based real-time controller.The actuator spacing of the PDSM measures 19.3 mm,equivalent to approximately 12.6 cm when mapped onto the primary mirror,significantly less than the voicecoil-based adaptive telescopes such as LBT,Magellan and VLT.As a result,stellar images with Strehl ratios above 0.49 in the R band have been obtained.To our knowledge,these are the highest R band images captured by an adaptive telescope with deformable secondary mirrors.Here,we report the system description and on-sky performance of this adaptive telescope. 展开更多
关键词 adaptive optics deformable secondary mirror visible imaging
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