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Identification and Molecular Mapping of the Rs Dm R Locus Conferring Resistance to Downy Mildew at Seedling Stage in Radish(Raphanus sativus L.)
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作者 XU Liang JIANG Qiu-wei +5 位作者 WU Jian WANG Yan GONG Yi-qin WANG Xian-li Limera Cecilia LIU Li-wang 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2014年第11期2362-2369,共8页
Downy mildew (DM), caused by the fungus Peronospora parasitica, is a destructive disease of radish (Raphanus sativus L.) worldwide. Host resistance has been considered as an attractive and environmentally friendly... Downy mildew (DM), caused by the fungus Peronospora parasitica, is a destructive disease of radish (Raphanus sativus L.) worldwide. Host resistance has been considered as an attractive and environmentally friendly approach to control the disease. However, the genetic mechanisms of resistance in radish to the pathogen remain unknown. To determine the inheritance of resistance to DM, F1, F2 and BC1F1 populations derived from reciprocal crosses between a resistant line NAU-dhp08 and a susceptible line NAU-qtbjq-06 were evaluated for their responses to DM at seedling stage. All F1 hybrid plants showed high resistance to DM and maternal effect was not detected. The segregation for resistant to susceptible individuals statistically iftted a 3:1 ratio in two F2 populations (F2(SR) and F2(RS)), and 1:1 ratio in two BC1F1 populations, indicating that resistance to DM at seedling stage in radish was controlled by a single dominant locus designated as RsDmR. A total of 1 972 primer pairs (1 036 SRAP, 628 RAPD, 126 RGA, 110 EST-SSR and 72 ISSR) were screened, and 36 were polymorphic between the resistant and susceptible bulks, and consequently used for genotyping individuals in the F2 population. Three markers (Em9/ga24370, NAUISSR826700 and Me7/em10400) linked to the RsDmR locus within a 10.0 cM distance were identiifed using bulked segregant analysis (BSA). The SRAP marker Em9/ga24370 was the most tightly linked one with a distance of 2.3 cM to RsDmR. These markers tightly linked to the RsDmR locus would facilitate marker-assisted selection and resistance gene pyramiding in radish breeding programs. 展开更多
关键词 Raphanus sativus L. molecular mapping downy mildew seedling-stage resistance marker assisted selection
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杉木秋播苗造林早期效果的研究
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作者 王源楠 《福建林学院学报》 CSCD 1990年第2期177-181,共5页
选用杉木秋播苗和春播苗进行造林效果的对比试验。在造林后的头3年内,分别测定其树高、地径、冠幅、分枝数、枝条总长和根冠等生长指标。结果表明,秋播苗幼林各项生长指标均比春播苗造林的幼林优势;秋播苗造林成活率也较春播苗造林提高... 选用杉木秋播苗和春播苗进行造林效果的对比试验。在造林后的头3年内,分别测定其树高、地径、冠幅、分枝数、枝条总长和根冠等生长指标。结果表明,秋播苗幼林各项生长指标均比春播苗造林的幼林优势;秋播苗造林成活率也较春播苗造林提高了5%,并可提早一年郁闭,减少抚育管理经费,有较高的经济效益。宜提倡杉木秋播苗造林,以提高造林质量。 展开更多
关键词 杉木 秋播苗 造林 早期效果
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