To study the effect of exogenous selenium on fruit quality in walnut(Juglans regia L.),8-year-old walnut(Qingxiang)was taken as the research object.In the fruit expansion stage,300 mg/L of sodium selenate,yeast seleni...To study the effect of exogenous selenium on fruit quality in walnut(Juglans regia L.),8-year-old walnut(Qingxiang)was taken as the research object.In the fruit expansion stage,300 mg/L of sodium selenate,yeast selenium and sodium selenite solutions were applied on the leaf of walnut,and the selenium levels in leaves,pericarp and kernel were determined at the ripening stage.The fruit quality,mineral nutrient content,antioxidant enzyme activity,and related genes’expression were analyzed.The results showed that the three exogenous selenium increased the selenium levels in leaves,pericarp and kernel of walnut.They also significantly increased fruit and kernel weights,and kernel linoleic acid,but markedly decreased kernel crude fat and saturated fatty acid.Selenium spraying promoted the absorption of mineral nutrients such as potassium and zinc,but inhibited the absorption of calcium,and had no significant effect on iron and magnesium in the kernel.Three exogenous selenium increased the activities of superoxide dismutase(SOD),peroxidase(POD),ascorbate peroxidase(APX),and catalase(CAT)significantly in the kernel.Except for sodium selenate treatment significantly reduced malondialdehyde(MDA)content in the kernel,the other two selenium sources treatments had no significant effect on MDA and hydrogen peroxide(H2O2)levels.They also increased the expression of JrCu/Zn-SOD,Jr2-Cys POD,JrCyt-APX and JrCAT in kernel,to different extents.These implies that,in the walnut fruit enlargement period,the foliar spraying selenium could increase the selenium content of walnut,affect the mineral nutrient absorption,improve the antioxidant capacity and related genes’expression,and reduce the degree of peroxide,and then improve the quality of fruit.Furthermore,yeast selenium showed the comprehensive effect of the best.展开更多
[Objectives]This study aimed to explore the effect of different concentrations of selenium fertilizer on the yield,selenium content and heavy metal contents of waxy maize,thereby providing reference for the scientific...[Objectives]This study aimed to explore the effect of different concentrations of selenium fertilizer on the yield,selenium content and heavy metal contents of waxy maize,thereby providing reference for the scientific application of selenium fertilizer on waxy maize.[Methods]Different varieties of waxy maize(Guitiannuo 611,Guinuo 615 and Guiheinuo 609)were used as test materials.They were sprayed with different concentrations(0,0.5,1.0,1.5 and 2.0 g/L)of water-soluble selenium fertilizer(Xinxibao)at the big flare stage,and the effect on the yield,selenium content and heavy metal contents of the waxy maize was analyzed.[Results]Foliar application of different concentrations of selenium fertilizer showed no significant effect on the yield and yield components of waxy maize.As the concentration of selenium fertilizer increased,the grain selenium content of different varieties of waxy maize increased.Among them,the increase of Guinuo 615 was the largest,of which the selenium content reached 0.265 mg/kg,2.82 times that of the control(water).The cadmium and arsenic contents of different varieties of waxy maize decreased with the increase of the concentration of selenium fertilizer sprayed.Among the waxy maize varieties,the cadmium and arsenic contents of Guitiannuo 611 dropped the most significantly,up to 52.94%and 77.78%.In waxy maize,the correlation coefficients between selenium content and cadmium,arsenic contents were-0.5509(P<0.05)and-0.8530(P<0.01),respectively.[Conclusions]Spraying exogenous selenium fertilizer had no obvious effect on the yield of waxy maize.However,the increase of the concentration of exogenous selenium fertilizer could significantly increase the selenium content and reduce the cadmium and arsenic contents in grains of different varieties of waxy maize.展开更多
With the improvement of living standards, people pay more attention to the agricultural products with health protection function, and the selenium-rich agricultural products attract more and more consumers. The main b...With the improvement of living standards, people pay more attention to the agricultural products with health protection function, and the selenium-rich agricultural products attract more and more consumers. The main biological role of selenium is to resist oxidation and inflammatory response, mainly focusing on resisting aging, preventing cardiovascular disease, protecting eyesight, counteracting or destroying the toxic properties, preventing cancer and thyroid disease. In most areas of China, there is a widespread shortage of selenium, thus producing selenium-rich agricultural products to provide natural selenium-rich health food to the areas in need of selenium, has gradually become a new hot spot of China's health food industry, but high content of selenium in food is detrimental to human body, even leads to selenium intoxication, and artificially adding inorganic selenium is difficult to guarantee that the selenium content of agricultural products is not exceeded. According to human body's daily demand for selenium in dietetics and the content of selenium in agricultural products in the Chinese food composition table, we put forward the recommendations on the standard of selenium in agricultural products, in order to provide the basis for China to formulate the health standard of selenium content in selenium-rich agricultural products.展开更多
Some common staple roots and tubers (cassava, yam, sweet potato and yellow yam) were analyzed for selenium content level using hydride generation—atomic absorption spectrophotometry (HG-ASS) technique. Results for du...Some common staple roots and tubers (cassava, yam, sweet potato and yellow yam) were analyzed for selenium content level using hydride generation—atomic absorption spectrophotometry (HG-ASS) technique. Results for duplicate analysis revealed that sweet potato has the highest mean selenium content (19.2 ± 5.20 μg/kg), followed by yellow yam (18.3 ± 6.97 μg/kg), then yam (13.6 ± 7.12 μg/kg) and cassava the least (13.0 ± 5.84 μg/kg). In comparing our results with the literature values, most of the results obtained in this work such as <1.00, 1.09, 1.91, 2.35 and 11.0 μg/kg were lower while a few others like 52.6, 54.2, 72.3 and 81.8 μg/kg were higher than literature values. The variations could be due to the type of species/variety, geographical location, total selenium concentration in the soil and its bioavailability. In view of the importance of Se to human health and considering the levels found in the staples investigated, we recommend that sweet potato be popularized as a staple, much more than cassava as it is now the case in Central Nigeria.展开更多
Nutrition is an important aspect of public health because it is linked to many significant diseases and health problems. This work is part of the promotion of traditional foods from the Democratic Republic of Congo in...Nutrition is an important aspect of public health because it is linked to many significant diseases and health problems. This work is part of the promotion of traditional foods from the Democratic Republic of Congo in general and in the province of Bandundu, in particular by assessing the selenium content and the antioxidant capacity of wild edible mushrooms. Methanolic extracts from selected mushrooms were characterized for their chemical fingerprint by TLC and their in vitro antioxidant activities using ABTS, DPPH assays. Phytochemical screening revealed the presence of alkaloids, free amines, steroids and terpenes in all species. However, Auricularia delicata and Pleurotus tuberregium contain flavonoids. All extracts displayed a good radical-scavenging activity at the concentration range 1 - 300 μg/mL in the following order: Auricularia delicata > Lentinus cf cladopus > Pleurotus tuberregium > Marasmius buzungolo > Schizophillum commune. Studied mushrooms showed the interest quantity of selenium and L. cf cladodus had the highest concentration. Moderate selenium content of these wild mushrooms associated with their important antioxidant activities could provide health benefits to Bandundu’s populations in protecting against oxidative damage under different conditions including konzo.展开更多
[Objectives]This study was conducted to explore the treatment methods of amino acid selenium fertilizer suitable for increasing the selenium content of mangoes and improving the quality of mangoes,so as to provide a t...[Objectives]This study was conducted to explore the treatment methods of amino acid selenium fertilizer suitable for increasing the selenium content of mangoes and improving the quality of mangoes,so as to provide a theoretical basis for the production of selenium-enriched mangoes.[Methods]With Tainong mango as a test material,the amino acid selenium foliar fertilizer was applied by eight treatment methods to investigate the changes of selenium,soluble solid,vitamin C,and titratable acid contents in mango flesh and peel.[Results]Spraying amino acid selenium foliar fertilizer increased the selenium content of mango flesh.The selenium contents of the treatment groups T2,T3,T4 and T5 reached the selenium-enriched standard,and the T2 treatment group had the highest selenium content(0.020 mg/kg).The selenium contents in the peel of all treatment groups were greater than the corresponding selenium content in the flesh.Except for T1,the vitamin C contents of other treatment groups sprayed with amino acid selenium fertilizer increased compared with the control,and that of the T2 treatment group increased significantly(P<0.05).Compared with the control,the soluble solids of the treatment groups sprayed with amino acid selenium fertilizer once also increased.The treatment method of spraying amino acid selenium fertilizer with a dosage of 12000 ml/hm2 once(T2)achieved the best effect of increasing the selenium content and improving the quality of mangoes.[Conclusions]This study provides a scientific basis for increasing the selenium content of mangoes and improving the current situation of insufficient selenium intake.展开更多
[Objectives]This study was conducted to choose a selenium-rich foliar fertilizer suitable for the selenium-rich fruit and vegetable industry in Guangxi.[Methods]With "Lingfeng" wine grapes as test materials,...[Objectives]This study was conducted to choose a selenium-rich foliar fertilizer suitable for the selenium-rich fruit and vegetable industry in Guangxi.[Methods]With "Lingfeng" wine grapes as test materials,such four kinds of selenium fertilizers as "Jufu Selenium"(JF),"Taishan Weed Fertilizer"(TS),"Zhongnong No.6"(ZN) and "Kangxibao"(KX) were used as exogenous selenium for the experiment of selenium-enriched grapes.[Results]The selenium contents in the grape fruit applied with the selenium fertilizers increased by 1.2-16.8 times compared with the control (CK),and JF and ZN had a better selenium-enriching effect and produced the fruit having the selenium contents reaching 75.85 and 45.79 μg/kg,respectively.Spraying selenium fertilizers can improve some quality indexes of grapes in that spraying JF on the leaves can significantly increase the total sugar content of the grapes while reducing the total acid content and spraying ZN significantly improved the vitamin C content of the fruit.With the delay of harvesting,the selenium content,total sugar content and total acid content of the "Lingfeng" grape fruit decreased,while the grapes sprayed with ZN showed the total sugar content increased slightly and the total acid decreased greatly.[Conclusions]It is suggested that if you plan to harvest at the right time,you can use "Jufu Selenium" as the exogenous fertilizer for selenium-enriched grapes;and if you want to postpone harvesting,you can use "Zhongnong No.6" as the selenium fertilizer.展开更多
Selenium is an element which participates in antioxidant enzymes. A medium and long term lack of such element is associated, mainly, with heart disease, joint and bone structure problems and thyroid activity. Selenium...Selenium is an element which participates in antioxidant enzymes. A medium and long term lack of such element is associated, mainly, with heart disease, joint and bone structure problems and thyroid activity. Selenium contents in blood reflect its ingestion and food content variation. It depends on soil characteristics, such as pH and selenium presence. There are few studies concerning selenium levels in food and blood in Brazil. Therefore, the objective of this research was to determine selenium content in a blood donor group in Rio de Janeiro state, conducted from December 2008 to March 2009. From the donated blood to Regional Blood Center of Campos dos Goytacazes, 4.0 mL were submitted to selenium analysis through atomic absorption spectrometry of hydride generation and organic matter oxidation wet basis with nitric and perchloric acid. Selenium content varied from 75 ± 16 ng·g-1 for females and 70 ± 21 ng·g-1 for males. There was no significant difference among selenium contents in blood from donors of different gender, age and residence. Seventy four percent of donors had selenium content in blood below 70 ng·mL-1, the lowest level for maximum selenoproteins production, according to the Nutritional Prevention Cancer.展开更多
This study was to find out the suitable application amount of plant-derived humic acid organic fertilizer for selenium-rich rice production in northern Guilin area by applying the fertilizer at different amounts.The r...This study was to find out the suitable application amount of plant-derived humic acid organic fertilizer for selenium-rich rice production in northern Guilin area by applying the fertilizer at different amounts.The results showed that plant-derived humic acid organic fertilizer could effectively activate selenium in selenium-rich soil,and then promote the uptake of selenium by rice.With costs,selenium enrichment and rice yield as the main evaluation indicators,A4 was chosen as the best treatment,that is,the application of 4 500 kg/hm^2 of plant-derived humic acid organic fertilizer as base fertilizer could achieve the best benefit.展开更多
Selenium(Se)is an essential mineral element for human and other animals,and has been proved to improve plant growth and development and tolerance to different abiotic stresses.Selenium biofortification is considered t...Selenium(Se)is an essential mineral element for human and other animals,and has been proved to improve plant growth and development and tolerance to different abiotic stresses.Selenium biofortification is considered to be a key strategy to increase the selenium content of edible parts of crops,which is helpful for improving human health.In this work,foliar fertilization with different concentrations and selenium forms was carried out on two wheat varieties at the flowering stage to compare the selenium enrichment effect of Na_(2)SeO_(3),methylselenized selenocysteine(MSC),methylselenized glucose(MSG)and methylselenized lactide(MSL)in wheat grains.Surprisingly,MSG was found to be the preferable fertilizer.After the application of MSG,the highest selenium content in wheat gains reached 6 mg/kg in this experiment,and the average selenium content was 2-4 times versus that of Na_(2)SeO_(3)application.Since MSG has high utilization rate and is easily available at relatively low cost,it can be employed as a potential selenium source for selenium biofortification to enhance the added value of agricultural industry.展开更多
Selenium is a trace element that can have both beneficial and harmful effects on aquatic life. The Aby Lagoon is a coastal environment in Côte d’Ivoire that receives selenium inputs from various natural and anth...Selenium is a trace element that can have both beneficial and harmful effects on aquatic life. The Aby Lagoon is a coastal environment in Côte d’Ivoire that receives selenium inputs from various natural and anthropogenic sources. The aim of this study was to assess the levels of selenium in the sediments of the Aby Lagoon and its tributaries, the Tanoe River and the Tendo Lagoon, and to examine the spatial and seasonal variations of selenium concentrations. Sediment samples were collected from different sites and seasons, and selenium concentrations were measured by atomic fluorescence spectrometry. The results showed that the average concentration of selenium in the sediments of the Aby Lagoon was 0.82 mg/kg, indicating moderate contamination. The concentration of selenium varied between sites and seasons, with higher values in the channel of the Tendo Lagoon and during the dry season. The study highlighted the complexity of selenium dynamics in aquatic ecosystems, and the need to take into account seasonal and spatial variability as well as interactions between environmental factors. The study also suggested potential ecotoxicological risks for some sensitive organisms in certain areas of the lagoon. This study contributes to the knowledge of the dynamics of selenium in lagoon ecosystems and to the assessment of the environmental risks associated with its presence.展开更多
Seed germination with selenium(Se)is promising for producing Se-biofortified foods.Mungbean(Vigna radiata(L.)Wilczek)sprout is freshly eaten as a salad dressed with sauce,making it superior for Se biofortification.Since...Seed germination with selenium(Se)is promising for producing Se-biofortified foods.Mungbean(Vigna radiata(L.)Wilczek)sprout is freshly eaten as a salad dressed with sauce,making it superior for Se biofortification.Since the Se safety range for the human body is extremely narrow,it is imperative to evaluate the genotypic responses of mungbean sprouts to Se.This study evaluated the Se enrichment capacity and interaction withflavonoids and antioxidant systems in sprouts of 20 mungbean germplasms.Selenium treatment was done by immersing mung-bean seeds in 20μM sodium selenite solution for 8 h.Afterward,the biomass,Se amounts,flavonoid(particularly vitexin and isovitexin)contents,antioxidant capacity,and key biosynthetic gene expressions were measured.Sprout Se content was 2.0-7.0μg g^(-1) DW among the 20 mungbean germplasms.Selenium treatment differentially affected the biomass,totalflavonoid,vitexin,isovitexin,antioxidant enzyme activities,and antioxidant capacities of the mungbean germplasms.Eight germplasms showed increased biomass(p<0.05),the highest increasing by 127%,but 13 did not phenotypically respond to Se treatment.Seven and six germplasms showed varied levels of vitexin and isovitexin increment after Se treatment,the highest measuring 2.67-and 2.87-folds for vitexin and isovitexin,respectively.Two mungbeanflavonoid biosynthesis genes,chalcone synthase(VrCHS)and chalcone isomerase(VrCHI)were significantly up-regulated in the germplasms with increased vitexin and isovitexin levels(p<0.05).Moreover,Se enrichment capacity was significantly correlated with the vitexin,isovitexin,and antiox-idant capacities.In conclusion,mungbean sprouts could be a useful Se-biofortified food,but the Se enrichment capacity and nutritional response must be determined for each germplasm before commercialization.展开更多
In the present study,we investigated the synergistic effects of targeted methotrexate-selenium nanostructure containing Myc decoy oligodeoxynucleotides along with X-irradiation exposure as a combination therapy on LNC...In the present study,we investigated the synergistic effects of targeted methotrexate-selenium nanostructure containing Myc decoy oligodeoxynucleotides along with X-irradiation exposure as a combination therapy on LNCaP prostate cancer cells.Myc decoy ODNs were designed based on the promoter of Bcl-2 gene and analyzed by molecular docking and molecular dynamics assays.ODNs were loaded on the synthesized Se@BSA@Chi-MTX nanostructure.The physicochemical characteristics of nanostructures were determined by FTIR,DLS,UV-vis,TEM,EDX,in vitro release,and hemolysis tests.Subsequently,the cytotoxicity properties of them with and without X-irradiation were investigated by uptake,MTT,cell cycle,apoptosis,and scratch assays on the LNCaP cell line.The results of DLS and TEM showed negative charge(−9 mV)and nanometer size(40 nm)for Se@BSA@Chi-DEC-MTX NPs,respectively.The results of FTIR,UV-vis,and EDX showed the proper interaction of different parts and the correct synthesis of nanoparticles.The results of hemolysis showed the hemocompatibility of this nanoparticle in concentrations less than 6 mg/mL.The ODNs release from the nanostructures showed a pH-dependent manner,and the release rate was 15%higher in acidic pH.The targeted Se@BSA@Chi-labeled ODN-MTX NPs were efficiently taken up by LNCaP cells by targeting the prostate-specific membrane antigen(PSMA).The significant synergistic effects of nanostructure(containing MTX drug)treatment along with X-irradiation showed cell growth inhibition,apoptosis induction(~57%),cell cycle arrest(G2/M phase),and migration inhibition(up to 90%)compared to the control.The results suggested that the Se@BSA@Chi-DEC-MTX NPs can potentially suppress the cell growth of LNCaP cells.This nanostructure system can be a promising approach for targeted drug delivery and chemoradiotherapy in prostate cancer treatment.展开更多
Purpose: In recent years, there has been concern in Japan about the increase in STDs among adolescents aged 15 - 19. In order to prevent STDs and guide adolescents toward desirable sexual behavior, this study’s goal ...Purpose: In recent years, there has been concern in Japan about the increase in STDs among adolescents aged 15 - 19. In order to prevent STDs and guide adolescents toward desirable sexual behavior, this study’s goal was to examine the actual state of STD prevention education taught by school nurses as part of the school curriculum, as well as the kinds of digital content they wish to have for teaching on the subject. Method: An anonymous, self-administered questionnaire was distributed by email and leaflet. Targeting the 100 valid responses received from the surveyed school nurses, descriptive statistics were made for each survey item and comparisons were made between the early- and mid-career groups based on years of experience. Results: 70.0% of respondents reported teaching about STDs in Health & Physical Education classes. School nurses in the early-career group used ready-made materials, while the mid-career group used both ready-made and self-made materials. 95% of respondents reported that they had little or no knowledge of STDs, while 84% reported that they were “good” or “fairly good” at teaching classes on sexuality. Both groups reported difficulty with the topics of “phimosis” and “nocturnal emissions” in the physiological category, “sexual behavior” in the “sex-adjacent” category, and “sexual and reproductive issues” in the psychosocial category. Respondents expressed a need for digital content covering the topics of “sexual violence”, “sexual abuse”, “sexually transmitted diseases”, and “how to turn down sex”. The mid-career group desired digital content for more items than the early-career group, with significant differences in the pathogens involved in sexually transmitted diseases and sex-adjacent items. 32.0% of respondents answered that they had done self-study for sex education classes in the past three years, and both groups desired self-study on “sexually transmitted diseases”, with the mid-career group significantly higher than the early-career group on “cervical cancer & HPV”, “emergency contraceptives”, and “media literacy”. Conclusion: Health & Physical Education is the main subject that covers STDs, and pre-existing materials are commonly used. It is expected that this is due to nurses’ busy schedules and the fact that only one school nurse is assigned to each school. The data collected suggested that nurse teachers want categorized digital content that can be used in the classes they are responsible for, rather than educational content on topics they are not comfortable with. In addition, given the changing environment surrounding sex, it was clear that they wish to understand the realities and needs of the high school students they teach, and desire self-study opportunities to improve their teaching methods.展开更多
Limited research has been conducted on the influences of fiber content on close-in blasting characteristics for ultrahigh-performance fiber-reinforced concrete(UHPFRC)beams.This paper aims to address this knowledge ga...Limited research has been conducted on the influences of fiber content on close-in blasting characteristics for ultrahigh-performance fiber-reinforced concrete(UHPFRC)beams.This paper aims to address this knowledge gap through experimental and mesoscale numerical methods.Experiments were conducted on ten UHPFRC beams built with varying steel fiber volumetric fractions subjected to close-in explosive conditions.Additionally,this study considered other parameters,such as the longitudinal reinforcement type and ratio.In the case of UHPFRC beams featuring normal-strength longitudinal reinforcement of diametersΦ12,Φ16,andΦ20,a reduction in maximum displacement by magnitudes of19.6%,19.5%,and 17.4%was observed,respectively,as the volumetric fractions of fiber increased from1.0%to 2.5%.In addition,increasing the longitudinal reinforcement ratio and using high-strength steel longitudinal reinforcement both significantly reduced the deformation characteristics and increase the blasting resistances of UHPFRC beams.However,the effects on the local crushing and spalling damage were not significant.A mesoscale finite element model,which considers the impacts of fiber parameters on UHPFRC beam behaviors,was also established and well correlated with the test findings.Nevertheless,parametric analyses were further conducted to examine the impacts of the steel fiber content and length and the hybrid effects of various types of microfibers and steel fibers on the blasting performance of UHPFRC beams.展开更多
Objective: To analyze the correlation between salivary cortisol content and anxiety and depression in pregnant and postpartum women. Method: 300 pregnant and postpartum women who were admitted from January 2021 to Dec...Objective: To analyze the correlation between salivary cortisol content and anxiety and depression in pregnant and postpartum women. Method: 300 pregnant and postpartum women who were admitted from January 2021 to December 2021 were selected as the research subjects. They were all tested with the Hamilton Anxiety Scale (HAMA) and the Edinburgh Postnatal Depression Scale (EPDS). 40 pregnant women with anxiety and depression were selected as the observation group, and 50 normal pregnant women were selected as the control group, adopting logistic regression analysis to investigate the correlation between salivary cortisol and postpartum anxiety and depression during pregnancy. Result: The salivary cortisol levels in the observation group were significantly higher than those in the control group before bedtime, after waking up the next day, 15 minutes after waking up, 30 minutes after waking up, 60 minutes after waking up, and 1 hour before lunch (p < 0.05). Maternal depression and anxiety were not related to age, weight, gestational age, negative events, or history of adverse pregnancy and childbirth (p > 0.05). Maternal depression and anxiety are closely related to salivary cortisol levels and educational background (p Conclusion: There is a close correlation between anxiety and depression and an increase in cortisol levels, suggesting that the salivary cortisol awakening response during pregnancy has a predictive effect on the occurrence of postpartum depression.展开更多
The study by Feng et al,explores treatment approaches for these coexisting conditions.It emphasizes the potential advantages of selenium and vitamin D supplementation but also raises methodological and patient selecti...The study by Feng et al,explores treatment approaches for these coexisting conditions.It emphasizes the potential advantages of selenium and vitamin D supplementation but also raises methodological and patient selection concerns.Findings indicate a complex interplay between interventions and disease markers,prompting the need for further research.Despite limitations,the study offers valuable insights into managing the intricate relationship between type 2 diabetes mellitus and Hashimoto's thyroiditis.The authors'contributions shed light on potential treatment avenues,although careful consideration of study design and patient characteristics is warranted for future investigations in this domain.展开更多
基金supported by the Science and Technology Planning Project of Shannan Science and Technology Bureau of Tibet(2021Z21008)the Hubei Province‘14th Five-Year’Major Science and Technology Aid Tibet Project(SCXX-XZCG-22016).
文摘To study the effect of exogenous selenium on fruit quality in walnut(Juglans regia L.),8-year-old walnut(Qingxiang)was taken as the research object.In the fruit expansion stage,300 mg/L of sodium selenate,yeast selenium and sodium selenite solutions were applied on the leaf of walnut,and the selenium levels in leaves,pericarp and kernel were determined at the ripening stage.The fruit quality,mineral nutrient content,antioxidant enzyme activity,and related genes’expression were analyzed.The results showed that the three exogenous selenium increased the selenium levels in leaves,pericarp and kernel of walnut.They also significantly increased fruit and kernel weights,and kernel linoleic acid,but markedly decreased kernel crude fat and saturated fatty acid.Selenium spraying promoted the absorption of mineral nutrients such as potassium and zinc,but inhibited the absorption of calcium,and had no significant effect on iron and magnesium in the kernel.Three exogenous selenium increased the activities of superoxide dismutase(SOD),peroxidase(POD),ascorbate peroxidase(APX),and catalase(CAT)significantly in the kernel.Except for sodium selenate treatment significantly reduced malondialdehyde(MDA)content in the kernel,the other two selenium sources treatments had no significant effect on MDA and hydrogen peroxide(H2O2)levels.They also increased the expression of JrCu/Zn-SOD,Jr2-Cys POD,JrCyt-APX and JrCAT in kernel,to different extents.These implies that,in the walnut fruit enlargement period,the foliar spraying selenium could increase the selenium content of walnut,affect the mineral nutrient absorption,improve the antioxidant capacity and related genes’expression,and reduce the degree of peroxide,and then improve the quality of fruit.Furthermore,yeast selenium showed the comprehensive effect of the best.
基金Key Research and Development Project of Guangxi(Gui Ke AB18221056)Guangxi Innovative Team Building Project in Earmarked Fund for China Agriculture Research System(nycytxgxcxtd-04-02)Special Fund for Innovation-driven Development in Guangxi(Gui Ke AA17204064)。
文摘[Objectives]This study aimed to explore the effect of different concentrations of selenium fertilizer on the yield,selenium content and heavy metal contents of waxy maize,thereby providing reference for the scientific application of selenium fertilizer on waxy maize.[Methods]Different varieties of waxy maize(Guitiannuo 611,Guinuo 615 and Guiheinuo 609)were used as test materials.They were sprayed with different concentrations(0,0.5,1.0,1.5 and 2.0 g/L)of water-soluble selenium fertilizer(Xinxibao)at the big flare stage,and the effect on the yield,selenium content and heavy metal contents of the waxy maize was analyzed.[Results]Foliar application of different concentrations of selenium fertilizer showed no significant effect on the yield and yield components of waxy maize.As the concentration of selenium fertilizer increased,the grain selenium content of different varieties of waxy maize increased.Among them,the increase of Guinuo 615 was the largest,of which the selenium content reached 0.265 mg/kg,2.82 times that of the control(water).The cadmium and arsenic contents of different varieties of waxy maize decreased with the increase of the concentration of selenium fertilizer sprayed.Among the waxy maize varieties,the cadmium and arsenic contents of Guitiannuo 611 dropped the most significantly,up to 52.94%and 77.78%.In waxy maize,the correlation coefficients between selenium content and cadmium,arsenic contents were-0.5509(P<0.05)and-0.8530(P<0.01),respectively.[Conclusions]Spraying exogenous selenium fertilizer had no obvious effect on the yield of waxy maize.However,the increase of the concentration of exogenous selenium fertilizer could significantly increase the selenium content and reduce the cadmium and arsenic contents in grains of different varieties of waxy maize.
基金Supported by Hunan University Innovation Team FoundationHunan Science and Technology Plan Project (2011NK3093)
文摘With the improvement of living standards, people pay more attention to the agricultural products with health protection function, and the selenium-rich agricultural products attract more and more consumers. The main biological role of selenium is to resist oxidation and inflammatory response, mainly focusing on resisting aging, preventing cardiovascular disease, protecting eyesight, counteracting or destroying the toxic properties, preventing cancer and thyroid disease. In most areas of China, there is a widespread shortage of selenium, thus producing selenium-rich agricultural products to provide natural selenium-rich health food to the areas in need of selenium, has gradually become a new hot spot of China's health food industry, but high content of selenium in food is detrimental to human body, even leads to selenium intoxication, and artificially adding inorganic selenium is difficult to guarantee that the selenium content of agricultural products is not exceeded. According to human body's daily demand for selenium in dietetics and the content of selenium in agricultural products in the Chinese food composition table, we put forward the recommendations on the standard of selenium in agricultural products, in order to provide the basis for China to formulate the health standard of selenium content in selenium-rich agricultural products.
文摘Some common staple roots and tubers (cassava, yam, sweet potato and yellow yam) were analyzed for selenium content level using hydride generation—atomic absorption spectrophotometry (HG-ASS) technique. Results for duplicate analysis revealed that sweet potato has the highest mean selenium content (19.2 ± 5.20 μg/kg), followed by yellow yam (18.3 ± 6.97 μg/kg), then yam (13.6 ± 7.12 μg/kg) and cassava the least (13.0 ± 5.84 μg/kg). In comparing our results with the literature values, most of the results obtained in this work such as <1.00, 1.09, 1.91, 2.35 and 11.0 μg/kg were lower while a few others like 52.6, 54.2, 72.3 and 81.8 μg/kg were higher than literature values. The variations could be due to the type of species/variety, geographical location, total selenium concentration in the soil and its bioavailability. In view of the importance of Se to human health and considering the levels found in the staples investigated, we recommend that sweet potato be popularized as a staple, much more than cassava as it is now the case in Central Nigeria.
文摘Nutrition is an important aspect of public health because it is linked to many significant diseases and health problems. This work is part of the promotion of traditional foods from the Democratic Republic of Congo in general and in the province of Bandundu, in particular by assessing the selenium content and the antioxidant capacity of wild edible mushrooms. Methanolic extracts from selected mushrooms were characterized for their chemical fingerprint by TLC and their in vitro antioxidant activities using ABTS, DPPH assays. Phytochemical screening revealed the presence of alkaloids, free amines, steroids and terpenes in all species. However, Auricularia delicata and Pleurotus tuberregium contain flavonoids. All extracts displayed a good radical-scavenging activity at the concentration range 1 - 300 μg/mL in the following order: Auricularia delicata > Lentinus cf cladopus > Pleurotus tuberregium > Marasmius buzungolo > Schizophillum commune. Studied mushrooms showed the interest quantity of selenium and L. cf cladodus had the highest concentration. Moderate selenium content of these wild mushrooms associated with their important antioxidant activities could provide health benefits to Bandundu’s populations in protecting against oxidative damage under different conditions including konzo.
基金Guangxi Science and Technology Major Project(GK AA17202037-2,GK AA17202026)Guangxi Mango Innovation Team Cultivation Post Project of National Modern Agriculture Industrial Technology System(nycytxgxcxtd-06-02)+4 种基金Special Fund of Central Government for Local Science and Technology Development(GK ZY19183007)Special Action of the Science and Technology Vanguard of Guangxi Academy of Agricultural Sciences(GNK JZ202016)Guangxi Key R&D Program(GK AB16380164)Guangxi Selenium-enriched Crop Experiment Station(GTS2016011)Fundamental Scientific Research Fund of Guangxi Academy of Agricultural Sciences(GNK 2020YM109)。
文摘[Objectives]This study was conducted to explore the treatment methods of amino acid selenium fertilizer suitable for increasing the selenium content of mangoes and improving the quality of mangoes,so as to provide a theoretical basis for the production of selenium-enriched mangoes.[Methods]With Tainong mango as a test material,the amino acid selenium foliar fertilizer was applied by eight treatment methods to investigate the changes of selenium,soluble solid,vitamin C,and titratable acid contents in mango flesh and peel.[Results]Spraying amino acid selenium foliar fertilizer increased the selenium content of mango flesh.The selenium contents of the treatment groups T2,T3,T4 and T5 reached the selenium-enriched standard,and the T2 treatment group had the highest selenium content(0.020 mg/kg).The selenium contents in the peel of all treatment groups were greater than the corresponding selenium content in the flesh.Except for T1,the vitamin C contents of other treatment groups sprayed with amino acid selenium fertilizer increased compared with the control,and that of the T2 treatment group increased significantly(P<0.05).Compared with the control,the soluble solids of the treatment groups sprayed with amino acid selenium fertilizer once also increased.The treatment method of spraying amino acid selenium fertilizer with a dosage of 12000 ml/hm2 once(T2)achieved the best effect of increasing the selenium content and improving the quality of mangoes.[Conclusions]This study provides a scientific basis for increasing the selenium content of mangoes and improving the current situation of insufficient selenium intake.
基金Supported by Guangxi Science and Technology Major Project(GK AA17202037-6)Vitis quinquangularis Rehd Test Station in Luocheng,Guangxi(G TS201418)+1 种基金Science and Technology Program of Wuming District,Nanning City(20160303)Special Fund for Basic Scientific Research of Guangxi Academy of Agricultural Sciences(GNK 2017YM30)
文摘[Objectives]This study was conducted to choose a selenium-rich foliar fertilizer suitable for the selenium-rich fruit and vegetable industry in Guangxi.[Methods]With "Lingfeng" wine grapes as test materials,such four kinds of selenium fertilizers as "Jufu Selenium"(JF),"Taishan Weed Fertilizer"(TS),"Zhongnong No.6"(ZN) and "Kangxibao"(KX) were used as exogenous selenium for the experiment of selenium-enriched grapes.[Results]The selenium contents in the grape fruit applied with the selenium fertilizers increased by 1.2-16.8 times compared with the control (CK),and JF and ZN had a better selenium-enriching effect and produced the fruit having the selenium contents reaching 75.85 and 45.79 μg/kg,respectively.Spraying selenium fertilizers can improve some quality indexes of grapes in that spraying JF on the leaves can significantly increase the total sugar content of the grapes while reducing the total acid content and spraying ZN significantly improved the vitamin C content of the fruit.With the delay of harvesting,the selenium content,total sugar content and total acid content of the "Lingfeng" grape fruit decreased,while the grapes sprayed with ZN showed the total sugar content increased slightly and the total acid decreased greatly.[Conclusions]It is suggested that if you plan to harvest at the right time,you can use "Jufu Selenium" as the exogenous fertilizer for selenium-enriched grapes;and if you want to postpone harvesting,you can use "Zhongnong No.6" as the selenium fertilizer.
文摘Selenium is an element which participates in antioxidant enzymes. A medium and long term lack of such element is associated, mainly, with heart disease, joint and bone structure problems and thyroid activity. Selenium contents in blood reflect its ingestion and food content variation. It depends on soil characteristics, such as pH and selenium presence. There are few studies concerning selenium levels in food and blood in Brazil. Therefore, the objective of this research was to determine selenium content in a blood donor group in Rio de Janeiro state, conducted from December 2008 to March 2009. From the donated blood to Regional Blood Center of Campos dos Goytacazes, 4.0 mL were submitted to selenium analysis through atomic absorption spectrometry of hydride generation and organic matter oxidation wet basis with nitric and perchloric acid. Selenium content varied from 75 ± 16 ng·g-1 for females and 70 ± 21 ng·g-1 for males. There was no significant difference among selenium contents in blood from donors of different gender, age and residence. Seventy four percent of donors had selenium content in blood below 70 ng·mL-1, the lowest level for maximum selenoproteins production, according to the Nutritional Prevention Cancer.
基金Supported by the Special Fund for the Construction of Guangxi Innovation Team of China Agriculture Research Systemthe Agricultural Key Science and Technology Project of Guangxi(201621,201604)+6 种基金the Program for the Scientific Research and Technology Development in Guangxi(Guike AD16380194)the Special Fund for the Innovation-Driven Development in Guangxi(Guike AA17202026)the Key Research and Development Program of Guangxi(Guike AB16380207)the Science Research and Technology Development Program of Guilin City(2016011202,20170116-4)the Science Research and Technology Development Program of Nanning City(20165370)the Special Fund for the Collaborative Innovation of Fundamental Research,Guangxi Academy of Agricultural Sciences(Guinongke 2017YZ03)the Fund for Science and Technology Development of Guangxi Academy of Agricultural Sciences(2017JM07)
文摘This study was to find out the suitable application amount of plant-derived humic acid organic fertilizer for selenium-rich rice production in northern Guilin area by applying the fertilizer at different amounts.The results showed that plant-derived humic acid organic fertilizer could effectively activate selenium in selenium-rich soil,and then promote the uptake of selenium by rice.With costs,selenium enrichment and rice yield as the main evaluation indicators,A4 was chosen as the best treatment,that is,the application of 4 500 kg/hm^2 of plant-derived humic acid organic fertilizer as base fertilizer could achieve the best benefit.
基金financially supported by the National Key R&D Program:Intergovernmental Key Items for International Scientific and Technological Innovation Cooperation(No.2018YFE0107700)the Opening fund of Jiangsu Key Laboratory of Crop Genetics and Physiology(No.YCSL202006)+1 种基金the Jiangsu Provincial Six Talent Peaks Project(No.XCL-090)the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘Selenium(Se)is an essential mineral element for human and other animals,and has been proved to improve plant growth and development and tolerance to different abiotic stresses.Selenium biofortification is considered to be a key strategy to increase the selenium content of edible parts of crops,which is helpful for improving human health.In this work,foliar fertilization with different concentrations and selenium forms was carried out on two wheat varieties at the flowering stage to compare the selenium enrichment effect of Na_(2)SeO_(3),methylselenized selenocysteine(MSC),methylselenized glucose(MSG)and methylselenized lactide(MSL)in wheat grains.Surprisingly,MSG was found to be the preferable fertilizer.After the application of MSG,the highest selenium content in wheat gains reached 6 mg/kg in this experiment,and the average selenium content was 2-4 times versus that of Na_(2)SeO_(3)application.Since MSG has high utilization rate and is easily available at relatively low cost,it can be employed as a potential selenium source for selenium biofortification to enhance the added value of agricultural industry.
文摘Selenium is a trace element that can have both beneficial and harmful effects on aquatic life. The Aby Lagoon is a coastal environment in Côte d’Ivoire that receives selenium inputs from various natural and anthropogenic sources. The aim of this study was to assess the levels of selenium in the sediments of the Aby Lagoon and its tributaries, the Tanoe River and the Tendo Lagoon, and to examine the spatial and seasonal variations of selenium concentrations. Sediment samples were collected from different sites and seasons, and selenium concentrations were measured by atomic fluorescence spectrometry. The results showed that the average concentration of selenium in the sediments of the Aby Lagoon was 0.82 mg/kg, indicating moderate contamination. The concentration of selenium varied between sites and seasons, with higher values in the channel of the Tendo Lagoon and during the dry season. The study highlighted the complexity of selenium dynamics in aquatic ecosystems, and the need to take into account seasonal and spatial variability as well as interactions between environmental factors. The study also suggested potential ecotoxicological risks for some sensitive organisms in certain areas of the lagoon. This study contributes to the knowledge of the dynamics of selenium in lagoon ecosystems and to the assessment of the environmental risks associated with its presence.
基金This study was supported by the Key Project of Natural Science Research for Colleges and Universities in Anhui Province(KJ2021A0533,2023AH050345)the Excellent Scientific Research and Innovation Team of Universities in Anhui Province(2022AH010029).
文摘Seed germination with selenium(Se)is promising for producing Se-biofortified foods.Mungbean(Vigna radiata(L.)Wilczek)sprout is freshly eaten as a salad dressed with sauce,making it superior for Se biofortification.Since the Se safety range for the human body is extremely narrow,it is imperative to evaluate the genotypic responses of mungbean sprouts to Se.This study evaluated the Se enrichment capacity and interaction withflavonoids and antioxidant systems in sprouts of 20 mungbean germplasms.Selenium treatment was done by immersing mung-bean seeds in 20μM sodium selenite solution for 8 h.Afterward,the biomass,Se amounts,flavonoid(particularly vitexin and isovitexin)contents,antioxidant capacity,and key biosynthetic gene expressions were measured.Sprout Se content was 2.0-7.0μg g^(-1) DW among the 20 mungbean germplasms.Selenium treatment differentially affected the biomass,totalflavonoid,vitexin,isovitexin,antioxidant enzyme activities,and antioxidant capacities of the mungbean germplasms.Eight germplasms showed increased biomass(p<0.05),the highest increasing by 127%,but 13 did not phenotypically respond to Se treatment.Seven and six germplasms showed varied levels of vitexin and isovitexin increment after Se treatment,the highest measuring 2.67-and 2.87-folds for vitexin and isovitexin,respectively.Two mungbeanflavonoid biosynthesis genes,chalcone synthase(VrCHS)and chalcone isomerase(VrCHI)were significantly up-regulated in the germplasms with increased vitexin and isovitexin levels(p<0.05).Moreover,Se enrichment capacity was significantly correlated with the vitexin,isovitexin,and antiox-idant capacities.In conclusion,mungbean sprouts could be a useful Se-biofortified food,but the Se enrichment capacity and nutritional response must be determined for each germplasm before commercialization.
基金Zanjan University of Medical Sciences supported the present study(Grant Number:A-12-1244-18).
文摘In the present study,we investigated the synergistic effects of targeted methotrexate-selenium nanostructure containing Myc decoy oligodeoxynucleotides along with X-irradiation exposure as a combination therapy on LNCaP prostate cancer cells.Myc decoy ODNs were designed based on the promoter of Bcl-2 gene and analyzed by molecular docking and molecular dynamics assays.ODNs were loaded on the synthesized Se@BSA@Chi-MTX nanostructure.The physicochemical characteristics of nanostructures were determined by FTIR,DLS,UV-vis,TEM,EDX,in vitro release,and hemolysis tests.Subsequently,the cytotoxicity properties of them with and without X-irradiation were investigated by uptake,MTT,cell cycle,apoptosis,and scratch assays on the LNCaP cell line.The results of DLS and TEM showed negative charge(−9 mV)and nanometer size(40 nm)for Se@BSA@Chi-DEC-MTX NPs,respectively.The results of FTIR,UV-vis,and EDX showed the proper interaction of different parts and the correct synthesis of nanoparticles.The results of hemolysis showed the hemocompatibility of this nanoparticle in concentrations less than 6 mg/mL.The ODNs release from the nanostructures showed a pH-dependent manner,and the release rate was 15%higher in acidic pH.The targeted Se@BSA@Chi-labeled ODN-MTX NPs were efficiently taken up by LNCaP cells by targeting the prostate-specific membrane antigen(PSMA).The significant synergistic effects of nanostructure(containing MTX drug)treatment along with X-irradiation showed cell growth inhibition,apoptosis induction(~57%),cell cycle arrest(G2/M phase),and migration inhibition(up to 90%)compared to the control.The results suggested that the Se@BSA@Chi-DEC-MTX NPs can potentially suppress the cell growth of LNCaP cells.This nanostructure system can be a promising approach for targeted drug delivery and chemoradiotherapy in prostate cancer treatment.
文摘Purpose: In recent years, there has been concern in Japan about the increase in STDs among adolescents aged 15 - 19. In order to prevent STDs and guide adolescents toward desirable sexual behavior, this study’s goal was to examine the actual state of STD prevention education taught by school nurses as part of the school curriculum, as well as the kinds of digital content they wish to have for teaching on the subject. Method: An anonymous, self-administered questionnaire was distributed by email and leaflet. Targeting the 100 valid responses received from the surveyed school nurses, descriptive statistics were made for each survey item and comparisons were made between the early- and mid-career groups based on years of experience. Results: 70.0% of respondents reported teaching about STDs in Health & Physical Education classes. School nurses in the early-career group used ready-made materials, while the mid-career group used both ready-made and self-made materials. 95% of respondents reported that they had little or no knowledge of STDs, while 84% reported that they were “good” or “fairly good” at teaching classes on sexuality. Both groups reported difficulty with the topics of “phimosis” and “nocturnal emissions” in the physiological category, “sexual behavior” in the “sex-adjacent” category, and “sexual and reproductive issues” in the psychosocial category. Respondents expressed a need for digital content covering the topics of “sexual violence”, “sexual abuse”, “sexually transmitted diseases”, and “how to turn down sex”. The mid-career group desired digital content for more items than the early-career group, with significant differences in the pathogens involved in sexually transmitted diseases and sex-adjacent items. 32.0% of respondents answered that they had done self-study for sex education classes in the past three years, and both groups desired self-study on “sexually transmitted diseases”, with the mid-career group significantly higher than the early-career group on “cervical cancer & HPV”, “emergency contraceptives”, and “media literacy”. Conclusion: Health & Physical Education is the main subject that covers STDs, and pre-existing materials are commonly used. It is expected that this is due to nurses’ busy schedules and the fact that only one school nurse is assigned to each school. The data collected suggested that nurse teachers want categorized digital content that can be used in the classes they are responsible for, rather than educational content on topics they are not comfortable with. In addition, given the changing environment surrounding sex, it was clear that they wish to understand the realities and needs of the high school students they teach, and desire self-study opportunities to improve their teaching methods.
基金supported by the National Natural Science Foundation of China(Grant No.12102050)the Open Fund of State Key Laboratory of Explosion Science and Technology(Grant No.SKLEST-ZZ-21-18)。
文摘Limited research has been conducted on the influences of fiber content on close-in blasting characteristics for ultrahigh-performance fiber-reinforced concrete(UHPFRC)beams.This paper aims to address this knowledge gap through experimental and mesoscale numerical methods.Experiments were conducted on ten UHPFRC beams built with varying steel fiber volumetric fractions subjected to close-in explosive conditions.Additionally,this study considered other parameters,such as the longitudinal reinforcement type and ratio.In the case of UHPFRC beams featuring normal-strength longitudinal reinforcement of diametersΦ12,Φ16,andΦ20,a reduction in maximum displacement by magnitudes of19.6%,19.5%,and 17.4%was observed,respectively,as the volumetric fractions of fiber increased from1.0%to 2.5%.In addition,increasing the longitudinal reinforcement ratio and using high-strength steel longitudinal reinforcement both significantly reduced the deformation characteristics and increase the blasting resistances of UHPFRC beams.However,the effects on the local crushing and spalling damage were not significant.A mesoscale finite element model,which considers the impacts of fiber parameters on UHPFRC beam behaviors,was also established and well correlated with the test findings.Nevertheless,parametric analyses were further conducted to examine the impacts of the steel fiber content and length and the hybrid effects of various types of microfibers and steel fibers on the blasting performance of UHPFRC beams.
文摘Objective: To analyze the correlation between salivary cortisol content and anxiety and depression in pregnant and postpartum women. Method: 300 pregnant and postpartum women who were admitted from January 2021 to December 2021 were selected as the research subjects. They were all tested with the Hamilton Anxiety Scale (HAMA) and the Edinburgh Postnatal Depression Scale (EPDS). 40 pregnant women with anxiety and depression were selected as the observation group, and 50 normal pregnant women were selected as the control group, adopting logistic regression analysis to investigate the correlation between salivary cortisol and postpartum anxiety and depression during pregnancy. Result: The salivary cortisol levels in the observation group were significantly higher than those in the control group before bedtime, after waking up the next day, 15 minutes after waking up, 30 minutes after waking up, 60 minutes after waking up, and 1 hour before lunch (p < 0.05). Maternal depression and anxiety were not related to age, weight, gestational age, negative events, or history of adverse pregnancy and childbirth (p > 0.05). Maternal depression and anxiety are closely related to salivary cortisol levels and educational background (p Conclusion: There is a close correlation between anxiety and depression and an increase in cortisol levels, suggesting that the salivary cortisol awakening response during pregnancy has a predictive effect on the occurrence of postpartum depression.
文摘The study by Feng et al,explores treatment approaches for these coexisting conditions.It emphasizes the potential advantages of selenium and vitamin D supplementation but also raises methodological and patient selection concerns.Findings indicate a complex interplay between interventions and disease markers,prompting the need for further research.Despite limitations,the study offers valuable insights into managing the intricate relationship between type 2 diabetes mellitus and Hashimoto's thyroiditis.The authors'contributions shed light on potential treatment avenues,although careful consideration of study design and patient characteristics is warranted for future investigations in this domain.
