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Evaluation of Resistance of Different Kiwifruit Varieties (Lines) to Canker Disease and Brown Spot Disease
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作者 Wenwen Su Chongpei Zheng +5 位作者 Zhencheng Han Chunguang Ren Di Wu Tao Li Yi Yang Weijie Li 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第6期1249-1261,共13页
Kiwifruit canker and brown spot are significant diseases affecting kiwis,caused by Pseudomonas syringae patho-genic variations(Pseudomonas syringae pv.Actinidiae(Psa))and Corynesporapolytica(Corynespora cassiicola).At ... Kiwifruit canker and brown spot are significant diseases affecting kiwis,caused by Pseudomonas syringae patho-genic variations(Pseudomonas syringae pv.Actinidiae(Psa))and Corynesporapolytica(Corynespora cassiicola).At present,the research on canker disease and brown spot disease mainly focuses on the isolation and identification of pathogenic bacteria,drug control,resistance gene mining and functional verification.Practice has proved that breeding disease resistant varieties are an effective method to control canker disease and brown spot disease.However,most existing cultivars lack genes for canker and brown spot resistance.Wild kiwifruit resources in nat-ure exhibit extensive genetic diversity due to prolonged natural selection,containing numerous resistance genes.But,due to insufficient understanding of the resistance of most kiwifruit varieties(lines)to canker disease and brown spot disease,some high-quality resources have not been fully utilized.The incidence of canker and brown spot of 18 kiwifruit cultivars(lines)was measured by inoculating isolated branches and leaves,and their resistance to canker and brown spot was analyzed according to the length,disease index,mean diameter,and systematic clustering.The results were as follows:Among 18 different kiwifruit varieties(lines)for canker disease,there were two highly resistant materials,eight disease-resistant materials,four disease-susceptible materials,and two highly susceptible materials.Moreover,regarding brown spot disease,there were one highly resistant material,five dis-ease-resistant materials,four susceptible materials,and three highly susceptible materials.Furthermore,four resources were resistant to both diseases.The outcomes provided a theoretical basis for breeding kiwifruit against canker and brown spot. 展开更多
关键词 kiwifruit canker disease brown spot resistance evaluation
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Identification and characterization of genes related to m^(6)A modification in kiwifruit using RNA-seq and ATAC-seq
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作者 Xiaoli Hu Tong Li +6 位作者 Changbin Xu Yanna Xu Congjun You Xinyi Li Jinli Gong Xiaolong Li Xuepeng Sun 《Horticultural Plant Journal》 SCIE CAS CSCD 2024年第5期1121-1133,共13页
N6-methyladenosine(m^(6)A)RNA modification is a conserved mechanism that regulates the fate of RNA across eukaryotic organisms.Despite its significance,a comprehensive analysis of m^(6)A-related genes in non-model pla... N6-methyladenosine(m^(6)A)RNA modification is a conserved mechanism that regulates the fate of RNA across eukaryotic organisms.Despite its significance,a comprehensive analysis of m^(6)A-related genes in non-model plants,such as kiwifruit,is lacking.Here,we identified 36 m^(6)A-related genes in the kiwifruit genome according to homology and phylogenetic inference.We performed bioinformatics and evolutionary analyses of the writer,eraser,and reader families of m^(6)A modification.Reanalysis of public RNA-seq data collected from samples under various biotic and abiotic stresses indicated that most m^(6)A-related genes were remarkably expressed under different conditions.Through construction of gene co-expression networks,we found significant correlations between several m^(6)A-related genes and transcription factors(TFs)as well as receptor-like genes during the development and ripening of kiwifruit.Furthermore,we performed ATAC-seq assays on diverse kiwifruit tissues to investigate the regulatory mechanisms of m^(6)A-related genes.We identified 10 common open chromatin regions that were present in at least two tissues,and these regions might serve as potential binding sites for MADS protein,C2H2 protein,and other predicted TFs.Our study offers comprehensive insights into the gene family of m^(6)A-related components in kiwifruit,which will lay foundation for exploring mechanisms of post-transcriptional regulation involved in development and adaptation of kiwifruit. 展开更多
关键词 kiwifruit N^(6)-methyladenosine Phylogenetic analysis CIS-ELEMENT Co-expression network
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Selenium-enriched and ordinary green tea extracts prevent high blood pressure and alter gut microbiota composition of hypertensive rats caused by high-salt diet 被引量:5
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作者 Meirong Wu Xiaobin Wu +3 位作者 Jiangxiong Zhu Fanglan Li Xinlin Wei Yuanfeng Wang 《Food Science and Human Wellness》 SCIE 2022年第3期738-751,共14页
High-salt diet is well recognized as a risk factor for hypertension,and dietary intervention plays a critical role in the prevention of hypertension.The current study investigated the effects of selenium-enriched gree... High-salt diet is well recognized as a risk factor for hypertension,and dietary intervention plays a critical role in the prevention of hypertension.The current study investigated the effects of selenium-enriched green tea(Se-GT)and ordinary green tea(GT)on prevention of hypertension of rats induced by high-salt diet,as well as their potential regulatory and mechanism.Our results showed that GT and Se-GT supplementations significantly prevented the increase of blood pressure(BP),activated the phosphoinosmde-3-kinase/protein kinase B(PI3K/Akt)signaling pathway,and regulated the gene expression related to BP,as well as improved the tissue damage like heart,liver,and kidneys.Besides,the key parameters associated with oxidative stress,inflammation and endothelial dysfunction were also altered by GT and Se-GT treatments.Importantly,GT or Se-GT administration adjusted the diversity and composition of the intestinal flora.Moreover,GT and Se-GT supplementations increased the abundance of beneficial bacteria and reduced the abundance of harmful or conditional pathogenic bacteria.More specifically,GT intake specifically and significantly enriched the relative abundance of Paraprevotella and Bacteroides,whereas Se-GT was characterized by specific and significant enrichment for Allobaculum and Bifidobacterium.Our results proved that dietary supplement of GT and Se-GT remarkably improved the vascular functions and effectively prevented tissue damage by regulation of intestinal flora,and thus preventing hypertension induced by high-salt diet. 展开更多
关键词 High-salt diet HYPERTENSION Green tea selenium-enriched green tea PI3K/Akt pathway Microbial profile
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Research on Strategies for the Development of Selenium-enriched Tea in Ankang City of Shaanxi Province in the Context of Targeted Poverty Alleviation 被引量:2
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作者 Jianbin YANG Hui ZHANG Yuan CHANG 《Asian Agricultural Research》 2020年第5期7-10,共4页
As an important symbol of agricultural and sideline products in Ankang City,selenium-enriched tea affects the development of regional agricultural and sideline industries.In this paper,the development status and probl... As an important symbol of agricultural and sideline products in Ankang City,selenium-enriched tea affects the development of regional agricultural and sideline industries.In this paper,the development status and problems of the selenium-enriched tea products of Ankang were analyzed in terms of capital,technology,management and sales mode.This research is of positive significance to the development of selenium-enriched products and other agricultural and sideline products of health value,and plays a very important role in promoting the cultivation of village-level characteristic industries and the effective realization of targeted poverty alleviation. 展开更多
关键词 Targeted poverty alleviation selenium-enriched tea industry Characteristic industries Development strategy
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Study on Extraction Method of Selenium-enriched Rice 被引量:1
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作者 Panxia LIANG Xiu LAN +6 位作者 Yongxian LIU Liping PAN Mengling NONG Ying XING Qing LIAO Shiyang LU Jinping CHEN 《Agricultural Biotechnology》 CAS 2018年第3期165-169,共5页
[Objectives] The aims were to optimize the extraction process of selenoproteins from selenium-enriched rice in Guangxi and provide references for the intensive processing and comprehensive utilization of selenium prot... [Objectives] The aims were to optimize the extraction process of selenoproteins from selenium-enriched rice in Guangxi and provide references for the intensive processing and comprehensive utilization of selenium protein resources. [Methods]Selenium-enriched rice was used as materials to extract selenoproteins by phosphate buffer extraction method and to optimize the extraction process of selenoproteins by using the orthogonal experiment. Proteins and selenium content was measured by Coomassie Brilliant Blue G-250 reagent and AFS( atomic fluorescence spectrometry) respectively. [Results] The most significant factor affecting extraction of rice Selenoproteins was extraction NaO H concentration,followed by the ratio of solid-liquid,temperature and then extraction time. The optimum extraction conditions of selenoproteins from rice were extraction temperature of 50 ℃,NaO H concentration of 0. 14 mol/L,extraction time of 5 h,and solid-liquid ratio of 1∶ 30. [Conclusions]The alkali extraction process optimized by orthogonal test could effectively improve the extraction rate of selenoproteins,and the optimized process parameters could be popularized and applied in practical production. 展开更多
关键词 selenium-enriched rice SELENOPROTEINS Orthogonal experiment Extraction efficiency
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Analysis of Influencing Factors in Production of Selenium-enriched Eggs 被引量:1
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作者 Hui HU Xu YANG +1 位作者 Yangchao CHEN Jiangong XIANG 《Asian Agricultural Research》 2022年第6期15-17,20,共4页
Selenium is an essential trace element for human health.Eating selenium-enriched eggs is conducive to easily and effectively solving the problem of selenium deficiency.In the production process of selenium-enriched eg... Selenium is an essential trace element for human health.Eating selenium-enriched eggs is conducive to easily and effectively solving the problem of selenium deficiency.In the production process of selenium-enriched eggs,different selenium sources,additive doses,feeding time,and the addition of other vitamins,trace elements,and methionine in the diet will influence the deposition of selenium in eggs.Through this review,it is intended to provide a reference for the practical,safe and economical production of selenium-enriched eggs. 展开更多
关键词 Laying hens selenium-enriched eggs Influencing factors Egg selenium conversion rate
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Recommendations for Sustainable Development of Selenium-enriched Functional Agriculture in Guangxi under the New Normal
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作者 Yongxian LIU 《Asian Agricultural Research》 2017年第11期23-27,共5页
Taking Scientific Outlook on Development as the guiding principle,this paper introduced the principles of innovation,coordination,green,openness and sharing. Based on gifted advantages of selenium-enriched soil and se... Taking Scientific Outlook on Development as the guiding principle,this paper introduced the principles of innovation,coordination,green,openness and sharing. Based on gifted advantages of selenium-enriched soil and selenium-enriched industry development in Guangxi,it discussed current practice,stressed green,ecological,characteristic demonstration,transformation and upgrading,and targeted poverty alleviation. Finally,it came up with perspective,practical,and operational strategies and recommendations for development of selenium-enriched industry,to provide reference for sustainable development of selenium-enriched functional industry in Guangxi. 展开更多
关键词 selenium-enrichment Functional agriculture Strategic project Development recommendations
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Effect of Soil Selenium on Nutritional Quality of Vegetables in the Selenium-enriched Areas of Qinghai Plateau
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作者 Yu ZHANG 《Asian Agricultural Research》 2018年第5期66-72,共7页
In order to improve the added value of the main vegetable products in Qinghai Province,we researched the effect of soil selenium content on vegetable quality in selenium-enriched area of Qinghai. Through the analysis ... In order to improve the added value of the main vegetable products in Qinghai Province,we researched the effect of soil selenium content on vegetable quality in selenium-enriched area of Qinghai. Through the analysis of Qinghai selenium-enriched soil selenium content and5 kinds of main vegetables corresponds to the total selenium,part of the mineral elements and soluble sugar and VC content,the effects of soil selenium levels on selenium absorption and nourishment quality of vegetables were researched. The results showed that he Qinghai selenium-enriched soil selenium content in the range of 100. 00-563. 00 μg/kg,among them,the soil samples with sufficient selenium and rich selenium accounted for 76. 53% and 18. 88%,respectively. Soil selenium content of garlic sampling area was relatively high,and soil selenium content of sugar beet sampling area was relatively low. Vegetable selenium content was in the range of 11. 00-340. 94 μg/kg,the average content of total selenium content of garlic was up to 170. 40 μg/kg,and the average content of the total selenium content of radish is 73. 00 μg/kg.90. 63% of the vegetables in the region reached the level of sufficient selenium,and 70. 31% reached the level of selenium enrichment. The average content of Ca and Mg was higher than the national average. There was no significant correlation between selenium content of soil and vegetable and nutritional quality. 展开更多
关键词 selenium-enriched areas of Qinghai VEGETABLES SELENIUM Nutritional quality
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Efficacy of a Kiwifruit Extract (PhenActivTM) on Gastrointestinal Tract Function: A Randomised Double-Blind Placebo-Controlled Study
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作者 David Briskey Alistair Rowan Mallard Amanda Rao 《Food and Nutrition Sciences》 2023年第12期1281-1295,共15页
Objective: Gastrointestinal (GI) discomfort is experienced by millions of people every day. This study aimed to evaluate the effect of PhenActiv<sup>TM</sup>, a novel green kiwifruit extract, on gastrointe... Objective: Gastrointestinal (GI) discomfort is experienced by millions of people every day. This study aimed to evaluate the effect of PhenActiv<sup>TM</sup>, a novel green kiwifruit extract, on gastrointestinal tract (GIT) function in otherwise healthy adults. Methods: 41 healthy adults with mild GI discomfort were enrolled in this double-blind, randomized, placebo-controlled study. Participants were randomized to either take 3.0 g/day of PhenActiv<sup>TM</sup> or a placebo for 6 weeks. Interviews were conducted at baseline, week 3 and week 6, with participants completing questionnaires regarding GI symptoms. Frequency of bowel movements was self-recorded daily. Results: There were no differences in daily and weekly defecation frequency and stool characteristics in either group. The active and placebo groups significantly improve GSRS scores (p , only the active group had a significant improvement in the IBSSS and PAC-QOL scores (p < 0.05) from baseline. Neither group had changes in sleep quality, quality of life and fatigue, plasma zonulin concentrations or macular pigment optical density scores. The product was well tolerated with no GI disturbances or adverse events being reported. Conclusion: Supplementation of 3.0 g/day of PhenActiv<sup>TM</sup> for 6 weeks did not improve defecation frequency or stool composition in healthy adults, but did improve perceived symptoms of GIT function, including symptoms of functional GIT disorders, IBS and constipation. The product was well tolerated and future trials investigating higher doses with more participants and/or a different population would be beneficial. 展开更多
关键词 kiwifruit Extract Gastrointestinal Tract Function STOOL Gastrointestinal Health Bowel Function Defecation Frequency
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Investigation and Identification of Fungal Diseases of Kiwifruit in Qiandongnan Prefecture
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作者 Guohui ZHANG Huanxian GU +2 位作者 Xiangyang LI Shengli SI Wenhua ZHANG 《Plant Diseases and Pests》 CAS 2023年第1期1-5,共5页
[Objectives]The paper was to investigate and identify the fungal diseases of wild and red heart kiwifruit in Qiandongnan Prefecture.[Methods]The pathogenic fungi were isolated from diseased leaves and fruits of wild a... [Objectives]The paper was to investigate and identify the fungal diseases of wild and red heart kiwifruit in Qiandongnan Prefecture.[Methods]The pathogenic fungi were isolated from diseased leaves and fruits of wild and red heart kiwifruit by tissue separation method.DNA sequencing was carried out by using the sequence analysis of ribosomal r DNA-ITS region,and molecular evolutionary trees were built by using MEGA 4.0 software.Finally,the pathogenic fungi were classified and identified by combining morphological observation.[Results]The main fungal diseases were anthracnose caused by Colletotrichum gloeosporioides on wild kiwifruit,fruit anthracnose caused by C.acutatum on red heart kiwifruit,leaf soft rot caused by Fusarium incarnatum on red heart kiwifruit,and brown spot caused by Alternaria alternata on red heart kiwifruit.[Conclusions]The study may provide some theoretical basis for the control of kiwifruit diseases in Qiandongnan Prefecture. 展开更多
关键词 Wild kiwifruit Red heart kiwifruit Fungal disease PATHOGEN IDENTIFICATION Qiandongnan Prefecture
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猕猴桃采后真菌病害生物防治研究进展 被引量:1
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作者 潘慧 龙超安 +2 位作者 钱国良 李黎 钟彩虹 《微生物学杂志》 CAS CSCD 北大核心 2024年第1期100-106,共7页
猕猴桃果实易受多种病原真菌的侵染,采后易发生软腐病、灰霉病、青霉病等严重危害果实品质的真菌性病害。传统的有效防治方法主要为采前使用化学杀菌剂,但其易污染环境并可能危害人类健康。目前,已有一系列生物防治方法被研究并报道,这... 猕猴桃果实易受多种病原真菌的侵染,采后易发生软腐病、灰霉病、青霉病等严重危害果实品质的真菌性病害。传统的有效防治方法主要为采前使用化学杀菌剂,但其易污染环境并可能危害人类健康。目前,已有一系列生物防治方法被研究并报道,这些方法可有效防控猕猴桃采后真菌病害:第一类为天然抑菌物质,包括植物提取物和其他天然物质;第二类为拮抗微生物,包括生防酵母、生防细菌、生防木霉,生防菌也能与物理及化学方法协同发挥作用。本文概述了各类生物防治方法在猕猴桃采后真菌病害绿色防控方面的研究进展及生防机理,并提出了目前存在的问题,最后展望了这一领域今后的研究方向。 展开更多
关键词 猕猴桃 生物防治 软腐病 灰霉病 青霉病
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氯吡脲处理对脐红猕猴桃果实品质的影响 被引量:1
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作者 张文 罗赛男 +4 位作者 王仁才 程小梅 曹胜 卜范文 周秀兰 《中国果树》 2024年第2期58-62,67,共6页
脐红为深受市场欢迎的黄肉红心芽变猕猴桃新品种,研究不同浓度氯吡脲处理对其果实品质的影响,对脐红猕猴桃生产具有重要指导意义。设置5、10 mg/L氯吡脲水溶液2个处理,在谢花后25 d进行浸果,以清水为对照(CK),于果实成熟期测定果实的外... 脐红为深受市场欢迎的黄肉红心芽变猕猴桃新品种,研究不同浓度氯吡脲处理对其果实品质的影响,对脐红猕猴桃生产具有重要指导意义。设置5、10 mg/L氯吡脲水溶液2个处理,在谢花后25 d进行浸果,以清水为对照(CK),于果实成熟期测定果实的外观品质、内在品质、矿质元素及水解氨基酸含量。结果表明:10 mg/L氯吡脲水溶液处理后的单果重、横径、侧径、可溶性固形物含量、固酸比均增幅最大,分别提高了32.26%、17.09%、12.45%、9.38%和18.73%;可滴定酸、维生素C、花色苷含量和钙元素含量则显著降低,分别降低了7.75%、17.05%、52.13%和28.30%。在单个氨基酸方面,胱氨酸和精氨酸等2种氨基酸含量随氯吡脲浓度的增加而显著降低,丝氨酸、甘氨酸、脯氨酸等3种氨基酸含量则显著提高,总氨基酸含量有所降低,但差异不显著。在不同类别氨基酸方面,甜味氨基酸含量随着氯吡脲浓度的增加而提高,鲜味氨基酸、芳香氨基酸含量则降低。综上,氯吡脲的施用可促进脐红猕猴桃果实膨大,增加果实风味,如提高可溶性固形物含量、降低可滴定酸含量,但高浓度处理降低了果实营养品质,如维生素C、花色苷、钙元素、鲜味氨基酸和芳香氨基酸含量。建议种植户合理施用氯吡脲,以促进脐红猕猴桃产业健康可持续发展。 展开更多
关键词 猕猴桃 氯吡脲 果实品质 氨基酸
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电感耦合等离子体发射光谱(ICP-OES)法测定陕西眉县猕猴桃矿物质含量及相关营养评价
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作者 王彩霞 刘宇 +2 位作者 胡佳薇 袁文婷 曹磊 《中国无机分析化学》 CAS 北大核心 2024年第10期1465-1470,共6页
为探究陕西眉县所产猕猴桃中矿物质含量及评价其相关营养价值。利用电感耦合等离子体发射光谱(ICP-OES)法对陕西眉县主产的徐香、翠香、红心、黄心四个品种猕猴桃中9种矿物质含量进行测定,并采用营养质量指数法(Index of nutritional qu... 为探究陕西眉县所产猕猴桃中矿物质含量及评价其相关营养价值。利用电感耦合等离子体发射光谱(ICP-OES)法对陕西眉县主产的徐香、翠香、红心、黄心四个品种猕猴桃中9种矿物质含量进行测定,并采用营养质量指数法(Index of nutritional quality,INQ)对矿物质元素进行营养评价,同时应用SPSS软件对9种矿物质和能量进行相关性分析。陕西眉县四个品种猕猴桃矿物质均以钾、磷、钙、镁四种元素为主,约占总矿物质的99.8%,四个品种中INQ均大于1的矿物质有钙、钾、镁、铜,其中钾的INQ最高在3.89~5.19。矿物总量最高的品种为翠香328 mg/100g,但营养质量指数最高的为徐香16.78(INQ_(总))。通过相关性分析表明各元素间存在着不同程度的相关性,其中钾与能量的相关系数最高为0.982。猕猴桃是一种高钾低钠的优质食品,不同品种猕猴桃矿物质元素的营养价值差异明显,矿物质营养密度最高的为翠香,其次为红心、黄心、徐香,但从营养价值指数方面来看,徐香最高,翠香次之,红心和黄心猕猴桃矿物营养水平稍逊。 展开更多
关键词 矿物质 猕猴桃 营养评价 相关性 ICP-OES
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不同品种猕猴桃多酚和类胡萝卜素组成的主成分分析
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作者 常虹 张翼钊 +4 位作者 钱建平 李文生 王云香 周家华 王宝刚 《食品研究与开发》 CAS 2024年第15期155-163,共9页
为研究不同品种猕猴桃果实的品质特征及多酚类和类胡萝卜素组成的区别,选取12种市售猕猴桃采用直接测量法对基本品质特征(硬度、可溶固形物、可滴定酸含量)进行测定,采用高效液相色谱法测定果实的多酚和类胡萝卜素组分含量,对不同品种... 为研究不同品种猕猴桃果实的品质特征及多酚类和类胡萝卜素组成的区别,选取12种市售猕猴桃采用直接测量法对基本品质特征(硬度、可溶固形物、可滴定酸含量)进行测定,采用高效液相色谱法测定果实的多酚和类胡萝卜素组分含量,对不同品种猕猴桃中多酚和类胡萝卜组成进行主成分分析。结果表明,12种猕猴桃果实品质特征、多酚组分含量和类胡萝卜素组分含量差异明显。海沃德果实较硬,硬度为1.10 kg/cm~2,翠香可溶性固形物含量较高,为18.78%,东红可滴定酸含量较高,为1.40%,翠玉维生素C含量较高,为207.90 mg/kg。猕猴桃果实中,单体酚以(-)-原儿茶酸含量最高,(-)-儿茶素含量最低;单体类胡萝卜素以β-胡萝卜素含量最高,α-胡萝卜素含量最低;猕猴桃果实中至少含有23种多酚,6种类胡萝卜素,其中奎宁酸、芦丁是含量最高的2种多酚,含量分别在74.09~304.16 mg/kg和3.58~31.63 mg/kg。金梅、翠香、海沃德、米良1号果实中的多酚组成最为复杂,至少有25种,其中β-胡萝卜素是含量最高的类胡萝卜素,含量在1.06~41.57 mg/kg。利用主成分分析法对12个猕猴桃品种进行全面的评价,得出4个主成分因子累计贡献率为68.609%,可以反映猕猴桃品质的大部分信息,不同品种猕猴桃多酚及类胡萝卜素含量差异明显。 展开更多
关键词 猕猴桃 多酚 类胡萝卜素 主成分分析 品质差异
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黄肉猕猴桃‘金实4号’果实采后蒂腐病病原菌的分离及鉴定
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作者 李忆 尹全 +2 位作者 张瑶 孙雷明 庄启国 《北方园艺》 CAS 北大核心 2024年第13期91-96,共6页
以猕猴桃新品种‘金实4号’采后病果为试材,取果实果蒂病组织分离腐烂病病原菌,按照柯赫氏法则确定分离病株的致病性,同时采用形态学结合分子生物学鉴定技术对病原菌种类进行鉴定,明确黄肉猕猴桃果实采后贮藏期引起腐烂的病菌种类,以期... 以猕猴桃新品种‘金实4号’采后病果为试材,取果实果蒂病组织分离腐烂病病原菌,按照柯赫氏法则确定分离病株的致病性,同时采用形态学结合分子生物学鉴定技术对病原菌种类进行鉴定,明确黄肉猕猴桃果实采后贮藏期引起腐烂的病菌种类,以期为黄肉猕猴桃采后腐烂病防治研究提供参考依据。结果表明:共有8种软腐病病原菌被鉴定,分别是拟茎点霉菌(Phomopsis sp.)、间座壳菌(Diaporthe cotoneastri)、小孢拟盘多毛孢(Pestalotiopsis microspora)、灰葡萄孢(Botrytis cinerea)、新壳梭孢(Neofusicoccum parvum)、链格孢(Alternaria sp.)、镰刀菌(Fusarium sp.)、葡萄座腔菌(Botryosphaeria dothidea)。 展开更多
关键词 ‘金实4号’猕猴桃 腐烂病 病原菌 鉴定
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不同美味猕猴桃花粉萌发活力及形态学研究
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作者 何利钦 王丽华 +3 位作者 郑晓琴 张茜 梁爽 李峤虹 《生物学杂志》 CAS CSCD 北大核心 2024年第4期78-82,共5页
为探究猕猴桃花粉萌发活力和花粉萌发前后的形态变化,以15份美味猕猴桃花粉为样本,采用离体培养方法测定花粉的萌发活力,利用扫描电镜观察离体培养前后5份雌株花粉和10份雄株花粉的形态变化。结果表明:15份花粉样本萌发率差异较大,5份... 为探究猕猴桃花粉萌发活力和花粉萌发前后的形态变化,以15份美味猕猴桃花粉为样本,采用离体培养方法测定花粉的萌发活力,利用扫描电镜观察离体培养前后5份雌株花粉和10份雄株花粉的形态变化。结果表明:15份花粉样本萌发率差异较大,5份雌株花粉均不萌发;10份雄株花粉中8号花粉样本的萌发率最高,为79.27%;9号花粉样本的萌发率最低,仅为22.85%。15份花粉样本离体培养前后扫描电镜观察发现:极面观基本相同,均为三裂圆形;赤道面观有差异,5份雌株花粉离体培养前后花粉形态不发生改变,均为近球形,而10份雄株花粉在萌发前为超长球形或长球形,萌发后均为近球形,极轴长度略有变小、赤道轴长变大、极轴长度与赤道轴长度比值变小、萌发沟宽度增宽,但萌发后的雄株花粉样本间各项形态学指标值差异较小。 展开更多
关键词 猕猴桃 花粉 离体培养 萌发活力 形态学
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猕猴桃便携式无损检测仪影响因素及其精度校准分析
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作者 张琛 郗笃隽 +2 位作者 裴嘉博 刘辉 汪末根 《江西农业学报》 CAS 2024年第4期53-58,共6页
为探究产地、成熟度对便携式可溶性固形物无损检测精度的影响,以同一品种3个产地的猕猴桃为试材,分别采用便携式无损检测仪和常规有损检测仪对其可溶性固形物含量进行测定和比较,并运用人工神经网络对其进行分析。结果表明,在使用便携... 为探究产地、成熟度对便携式可溶性固形物无损检测精度的影响,以同一品种3个产地的猕猴桃为试材,分别采用便携式无损检测仪和常规有损检测仪对其可溶性固形物含量进行测定和比较,并运用人工神经网络对其进行分析。结果表明,在使用便携式无损检测仪对猕猴桃可溶性固形物检测时,3个产地的无损检测R2分别为0.26、0.52、0.61,成熟度会对其检测精度造成一定影响,但在不同产地间没有稳定、统一的规律;基于人工神经网络学习、建模产生的随机预测值可将R2提高5%~49%。因此,可利用预测值来作为中间校准程序,以辅助提高便携式无损检测精度。 展开更多
关键词 猕猴桃 可溶性固形物 便携式无损检测 精度 人工神经网络
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猕猴桃9个种41份种质花粉形态观察与分析
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作者 何利钦 王丽华 +3 位作者 庄启国 张瑶 王凤琪 张茜 《中国南方果树》 北大核心 2024年第4期132-139,共8页
为了解猕猴桃属植物间的亲缘关系,以9个猕猴桃种41份种质的花粉为供试材料,采用扫描电镜结合ImageJ图像处理软件对花粉进行形态学观测,并根据花粉极轴长度(P)、赤道轴长度(E)、萌发沟长度、萌发沟宽度、极轴长度/赤道轴长度(P/E)和大小(... 为了解猕猴桃属植物间的亲缘关系,以9个猕猴桃种41份种质的花粉为供试材料,采用扫描电镜结合ImageJ图像处理软件对花粉进行形态学观测,并根据花粉极轴长度(P)、赤道轴长度(E)、萌发沟长度、萌发沟宽度、极轴长度/赤道轴长度(P/E)和大小(P×E)指标对供试材料进行聚类分析。结果表明,供试花粉均为单粒花粉,均以单粒、等极和辐射对称形式存在。极轴长度范围在21.50~33.40μm之间,赤道轴长度范围在9.87~16.24μm之间,萌发沟长度范围在17.53~29.08μm之间,萌发沟脊宽度范围在0.47~2.03μm之间,花粉粒大小(极轴×赤道轴)范围在218.68~543.18μm^(2)之间,极轴长度/赤道轴长度范围为1.36~2.26,花粉形状为超长球形或长球形,萌发孔类型均为三孔沟,极面观为三裂圆形,均为N3P4C5型。外壁纹饰分为波纹状、颗粒状和疣状,占样品总数的比例分别为63.41%、19.51%和17.08%。在遗传距离为7.5时,供试材料可以分为3个类群;在遗传距离为1.5时,供试材料可以分为6个类群,其中,中华猕猴桃被分为了4类。在两个遗传距离时,四萼猕猴桃均单独分为一类。 展开更多
关键词 猕猴桃 花粉形态 扫描电镜 聚类分析
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基于无人机多光谱的猕猴桃园冠层叶绿素含量检测方法
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作者 霍迎秋 赵士超 +2 位作者 赵国淇 孙江昊 胡少军 《农业机械学报》 EI CAS CSCD 北大核心 2024年第9期297-307,共11页
为实现对猕猴桃园区果树整体生长健康状况的快速、大规模监测,以猕猴桃园冠层叶片为研究对象,基于无人机拍摄果园多光谱图像,然后利用Pix4Dmapper软件拼接多光谱图像,获取果园的正射影像图,并进行辐射校正。切分正射影像为420个区域图... 为实现对猕猴桃园区果树整体生长健康状况的快速、大规模监测,以猕猴桃园冠层叶片为研究对象,基于无人机拍摄果园多光谱图像,然后利用Pix4Dmapper软件拼接多光谱图像,获取果园的正射影像图,并进行辐射校正。切分正射影像为420个区域图像作为样本,采用最大类间方差法(Otsu)分割样本图像的冠层叶片与土壤背景,并实测每个样本的冠层SPAD值,构建冠层叶片多光谱数据集。采用箱线图法对数据集进行异常值检测,剔除异常样本;然后利用多光谱图像多通道的数据特点,提取图像的相邻通道变化率和23种常用植被指数,以及二者组合作为样本特征值,接着利用CARS、LARS、IRIV等3种特征筛选算法优选特征,分别结合偏最小二乘回归(PLSR)、支持向量回归(SVR)、岭回归(RR)、多元线性回归(MLR)和极限梯度提升树(XGBoost)、最小绝对收缩和选择算子回归(Lasso)、随机森林回归(RFR)、高斯过程回归(GPR)等8种方法构建模型,识别猕猴桃园冠层SPAD值;最后对比分析以不同样本特征构建的24个模型的性能,实验结果表明:以相邻通道变化率为特征建立的模型中,GPR模型性能最好,R^(2)、RMSE分别为0.770、3.044;以植被指数和相邻通道变化率组合特征建立的模型中,GPR模型性能也最好,R^(2)、RMSE分别为0.783、2.957;以植被指数为数据特征建立的XGBoost模型性能最优,R^(2)、RMSE分别为0.787、2.933;因此基于无人机遥感的智能检测模型能够对果园冠层叶绿素含量进行准确评估。 展开更多
关键词 猕猴桃园 叶绿素含量 多光谱 机器学习 无人机
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3株生防菌对猕猴桃软腐病的作用
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作者 王丽 侯珲 +3 位作者 涂洪涛 黄天祥 柳友亮 袁洪波 《中国南方果树》 北大核心 2024年第3期233-240,共8页
为探究生防菌对猕猴桃软腐病的作用,选用前期获得的3株生防菌(贝莱斯芽孢杆菌P2-1、耐盐芽孢杆菌PL7和多黏芽孢杆菌NL4)进行室内抑菌试验、猕猴桃软腐病接种和自然发病防治试验,并研究了3株生防菌对猕猴桃果实病程相关基因表达的影响。... 为探究生防菌对猕猴桃软腐病的作用,选用前期获得的3株生防菌(贝莱斯芽孢杆菌P2-1、耐盐芽孢杆菌PL7和多黏芽孢杆菌NL4)进行室内抑菌试验、猕猴桃软腐病接种和自然发病防治试验,并研究了3株生防菌对猕猴桃果实病程相关基因表达的影响。试验结果表明,菌株P2-1、PL7和NL4对猕猴桃软腐病菌(Botryosphaeria dothidea)菌丝生长的抑制率分别为83.49%、74.82%和49.33%,P2-1的抑制效果最好,PL7次之,NL4最差;3株生防菌对接种猕猴桃软腐病均具有抑制作用,其中以P2-1的效果最好,PL7次之,NL4最差;3株生防菌均能显著降低猕猴桃软腐病自然发病率,其中以P2-1处理的发病率最低;3株生防菌处理均对猕猴桃果实品质无影响;3株生防菌处理猕猴桃果实后,果实中基因PR1、PR5和SOD的表达显著上调。研究结果可为菌株P2-1、PL7和NL4开发为防治猕猴桃软腐病的生物制剂提供参考。 展开更多
关键词 猕猴桃软腐病 葡萄座腔菌 生防菌 贝莱斯芽孢杆菌 耐盐芽孢杆菌 多黏芽孢杆菌
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