[Objective] The aim of this study is to isolate Chlorella vulgaris(chlorella)and extract its genomic DNA.[Method] Both the dilution method and drip method were employed to isolate chlorella from lake water samples;the...[Objective] The aim of this study is to isolate Chlorella vulgaris(chlorella)and extract its genomic DNA.[Method] Both the dilution method and drip method were employed to isolate chlorella from lake water samples;the conditions for culturing chlorella were optimized and its genomic DNA was extracted by improved CTAB method and SDS method.[Result] The proper conditions for chlorella culture were as following:temperature 20-25 ℃,illumination 4.39-5.86 W/m2 and rotational speed 100-150r/min;improved CTAB method was suitable for extracting genomic DNA from chlorella.[Conclusion] The study is helpful to study the chlorella at molecular level and promote the exploitation and utilization of chlorella resources.展开更多
[ Objective] This paper was to evaluate the methods of breaking stiffness cell wall of Chlorella sp. and extracting and testing functional antioxidant by ethanol and DPPH method separately. [Method] Extractions were p...[ Objective] This paper was to evaluate the methods of breaking stiffness cell wall of Chlorella sp. and extracting and testing functional antioxidant by ethanol and DPPH method separately. [Method] Extractions were performed at different extraction times （60, 120 and 180 min） at 37 ℃. And the radical scavenging activity of Chlorella spo extract was assayed by the DPPH （ 1,1-Diphenyl-2- picrylhydrazy[） method. [ Result ] The optimal conditions for ethanol extraction from Chlorella sp. were ethanol concentration of 90%, substrate consistency of 5% （w/v） and treating time of 180 min, under which, a concentration of 135.5 mg/L was obtained. Antioxidant pigments obtained from alcoholic extraction from Chlorella sp. showed high free radical scavenging ability. The efficiency increased with increasing concentration of solid microalgae powder, which could reach to 88%. [ Conclusion] Ethanol extraction method is simple and feasible. However, the efficiency of extraction is not high enough, which will limit the yield of antioxidant production from economic prospective.展开更多
Microalgae,a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical,bioenergy and food applications.This study aims to investigate the sugaring-out effect on the ...Microalgae,a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical,bioenergy and food applications.This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga,Chlorella sorokiniana CY1 which was assisted with sonication.A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides,glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein).The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases,as the yield of protein increased to 77%.The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well.The optimum protein productivity was obtained with 10s of resting time in between sonication.Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode.The optimum condition for protein extraction were found as followed:200g/L glucose as bottom phase with volume ratio of 1:1.25,10s of resting time for ultrasonication,5s of ultrasonication in pulse mode and 0.25g of biomass weight.The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future.展开更多
Steam pretreatment was employed to disrupt Microalgal cells for lipids extraction.Effects of steam pretreatment on microstructure of microalgal cells were investigated through scanning electron microscopy(SEM)and tran...Steam pretreatment was employed to disrupt Microalgal cells for lipids extraction.Effects of steam pretreatment on microstructure of microalgal cells were investigated through scanning electron microscopy(SEM)and transmission electron microscopy(TEM).Effect of treatment on lipid extraction was also studied.Microalgal cell walls were distorted after steam pretreatment due to the hydrolysis of organic macromolecules contained in cell wall.Maximum curvature was increased from 1.88×10^(-6) m^(-1) to 1.43×10^(-7) m^(-1) after treatment with the steam at 130℃.The fractal dimension of microalgal cells increased from 1.25 to 1.30 after pretreatment for 15 min,and further increased to 1.47 when the pretreatment time was increased to 60 min.Increased steam pretreatment temperature and time enhanced the hydrolysis of organic macromolecules,and finally destroyed microalgal cell walls at pretreatment temperature of 130℃and pretreatment time of 60 min.Lipid extracted from wet microalgal was significantly increased(2.1-fold)after pretreatment.展开更多
在温度为70℃、功率为600 W、提取时间为30 m in的条件下,海水小球藻中的多糖可以得到较好的提取。经过优化,当超声波作用时间为90 m in、超声波功率为300 W、水解液为1 mol/L乙酸时,小球藻多糖的超声水解效果最好。碱提、水提、酸提海...在温度为70℃、功率为600 W、提取时间为30 m in的条件下,海水小球藻中的多糖可以得到较好的提取。经过优化,当超声波作用时间为90 m in、超声波功率为300 W、水解液为1 mol/L乙酸时,小球藻多糖的超声水解效果最好。碱提、水提、酸提海水小球藻多糖中的单糖组成,经过GC-MS分析,它们的单糖组成各不相同。碱提粗多糖中所含单糖为鼠李糖、L-岩藻糖、D-阿拉伯糖、D(+)-木糖、D-甘露糖、D-葡萄糖、D-半乳糖,各单糖百分含量分别为20.68%、0.32%、1.0%、2.20%、4.58%、50.28%、20.91%(W/W);水提海水小球藻粗多糖中含鼠李糖、核糖、岩藻糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖8种单糖,它们百分比分别为2.32、2.36、0.24、0.89、0.56、3.44、78.21、11.99%(W/W);酸提粗多糖中所含单糖为鼠李糖、核糖、岩藻糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖,各单糖百分含量分别为2.75、1.75、0.23、1.04、0.61、5.99、74.33、13.30%(W/W)。本实验所建立的微波辅助提取、超声水解多糖、GC-MS分析多糖中的单糖组成等方法,具有简便、快速、准确的特点。可用于海藻和其它植物中的多糖研究。展开更多
基金Supported by the High-level Talents Start-up Fund of Shihezi University(07002-500002061401)~~
文摘[Objective] The aim of this study is to isolate Chlorella vulgaris(chlorella)and extract its genomic DNA.[Method] Both the dilution method and drip method were employed to isolate chlorella from lake water samples;the conditions for culturing chlorella were optimized and its genomic DNA was extracted by improved CTAB method and SDS method.[Result] The proper conditions for chlorella culture were as following:temperature 20-25 ℃,illumination 4.39-5.86 W/m2 and rotational speed 100-150r/min;improved CTAB method was suitable for extracting genomic DNA from chlorella.[Conclusion] The study is helpful to study the chlorella at molecular level and promote the exploitation and utilization of chlorella resources.
文摘[ Objective] This paper was to evaluate the methods of breaking stiffness cell wall of Chlorella sp. and extracting and testing functional antioxidant by ethanol and DPPH method separately. [Method] Extractions were performed at different extraction times （60, 120 and 180 min） at 37 ℃. And the radical scavenging activity of Chlorella spo extract was assayed by the DPPH （ 1,1-Diphenyl-2- picrylhydrazy[） method. [ Result ] The optimal conditions for ethanol extraction from Chlorella sp. were ethanol concentration of 90%, substrate consistency of 5% （w/v） and treating time of 180 min, under which, a concentration of 135.5 mg/L was obtained. Antioxidant pigments obtained from alcoholic extraction from Chlorella sp. showed high free radical scavenging ability. The efficiency increased with increasing concentration of solid microalgae powder, which could reach to 88%. [ Conclusion] Ethanol extraction method is simple and feasible. However, the efficiency of extraction is not high enough, which will limit the yield of antioxidant production from economic prospective.
基金Supported by the University of Malaya,Kuala Lumpur,Malaysia under SATU joint research scheme(No.ST004-2017)the International Cooperation Seeds Funding of Nanjing Agricultural University(No.2018-AH-04)
文摘Microalgae,a sustainable source of multi beneficial components has been discovered and could be utilised in pharmaceutical,bioenergy and food applications.This study aims to investigate the sugaring-out effect on the recovery of protein from wet green microalga,Chlorella sorokiniana CY1 which was assisted with sonication.A comparison of monosaccharides and disaccharides as one of the phaseforming constituents shows that the monosaccharides,glucose was the most suitable sugar in forming the phases with acetonitrile to enhance the production of protein(52% of protein).The protein productivity of microalgae was found to be significantly influenced by the volume ratio of both phases,as the yield of protein increased to 77%.The interval time between the sonication as well as the sonication modes were influencing the protein productivity as well.The optimum protein productivity was obtained with 10s of resting time in between sonication.Pulse mode of sonication was suitable to break down the cell wall of microalgae compared to continuous mode as a lower protein yield was obtained with the application of continuous mode.The optimum condition for protein extraction were found as followed:200g/L glucose as bottom phase with volume ratio of 1:1.25,10s of resting time for ultrasonication,5s of ultrasonication in pulse mode and 0.25g of biomass weight.The high yield of protein about 81% could be obtained from microalgae which demonstrates the potential of this source and expected to play an important role in the future.
基金supported by the National Key Research and Development Program-China(2017YFE0122800)Shandong Provincial Natural Science Foundation(ZR2019MC060)+1 种基金Key Research and Development Program of Jining City(2018ZDGH024)a Project of Shandong Province Higher Educational Science and Technology Program(J17KA095)。
文摘Steam pretreatment was employed to disrupt Microalgal cells for lipids extraction.Effects of steam pretreatment on microstructure of microalgal cells were investigated through scanning electron microscopy(SEM)and transmission electron microscopy(TEM).Effect of treatment on lipid extraction was also studied.Microalgal cell walls were distorted after steam pretreatment due to the hydrolysis of organic macromolecules contained in cell wall.Maximum curvature was increased from 1.88×10^(-6) m^(-1) to 1.43×10^(-7) m^(-1) after treatment with the steam at 130℃.The fractal dimension of microalgal cells increased from 1.25 to 1.30 after pretreatment for 15 min,and further increased to 1.47 when the pretreatment time was increased to 60 min.Increased steam pretreatment temperature and time enhanced the hydrolysis of organic macromolecules,and finally destroyed microalgal cell walls at pretreatment temperature of 130℃and pretreatment time of 60 min.Lipid extracted from wet microalgal was significantly increased(2.1-fold)after pretreatment.
文摘在温度为70℃、功率为600 W、提取时间为30 m in的条件下,海水小球藻中的多糖可以得到较好的提取。经过优化,当超声波作用时间为90 m in、超声波功率为300 W、水解液为1 mol/L乙酸时,小球藻多糖的超声水解效果最好。碱提、水提、酸提海水小球藻多糖中的单糖组成,经过GC-MS分析,它们的单糖组成各不相同。碱提粗多糖中所含单糖为鼠李糖、L-岩藻糖、D-阿拉伯糖、D(+)-木糖、D-甘露糖、D-葡萄糖、D-半乳糖,各单糖百分含量分别为20.68%、0.32%、1.0%、2.20%、4.58%、50.28%、20.91%(W/W);水提海水小球藻粗多糖中含鼠李糖、核糖、岩藻糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖8种单糖,它们百分比分别为2.32、2.36、0.24、0.89、0.56、3.44、78.21、11.99%(W/W);酸提粗多糖中所含单糖为鼠李糖、核糖、岩藻糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖,各单糖百分含量分别为2.75、1.75、0.23、1.04、0.61、5.99、74.33、13.30%(W/W)。本实验所建立的微波辅助提取、超声水解多糖、GC-MS分析多糖中的单糖组成等方法,具有简便、快速、准确的特点。可用于海藻和其它植物中的多糖研究。
