Silver nanoparticles have mainly been studied for their antimicrobial potential against bacteria, but have also proven to be active against several types of viruses, even against pebrine as well. Methods: Petri dishes...Silver nanoparticles have mainly been studied for their antimicrobial potential against bacteria, but have also proven to be active against several types of viruses, even against pebrine as well. Methods: Petri dishes and transmission electronic microscope were applied to observe silver nanoparticles against bacteria strains and nuclear polyhedral viruses (NPV) and cytoplasmic polyhedral viruses (CPV). For biological test, 108 CFU/mL NPV with strong pathogenicity, and the NPV samples treated with 1:100 ratio of “Sumerian Silver” for 8 hrs were sprayed on the mulberry leaves, respectively, for silkworm rearing. Conclusion: Silver nanoparticle solution showed a strong bactericidal effect against both G+ and G﹣bacterial pathogenic strains from Bombyx mori and mulberry. Under transmission electronic microscope (TEM), Bacillus sp. Samples showed light particles in the cells when treated with silver nanoparticles, in contrast, untreated samples showed homogeneity contents. Further, NPV particles showed no significant differences between treated and untreated samples, but CPV showed strong effects that almost all CPV were collapsed. For biological test, “Sumerian Silver” treated NPV showed no diseased silkworm but nearly all silkworms were dead with no treatment. It seems like that silver nanoparticles were proved to be more effective against CPV than that of NPV. The significant differences between two antivirus or virucidal mechanism should be greatly aroused the scientific interest.展开更多
With many years of benign development,Dongtai City has established a complete technical system for the sericulture industry,an advanced industrialized operation mode,a mature operation mechanism of trade,industry and ...With many years of benign development,Dongtai City has established a complete technical system for the sericulture industry,an advanced industrialized operation mode,a mature operation mechanism of trade,industry and agriculture,and a perfect social service mode.The production level of sericulture in Dongtai City is constantly improved. However,there are still problems such as small scale of individual household,slow technological progress,low level of industrial mechanization and labor saving,and hidden dangers in safe production of sericulture. It is recommended to fully build mulberry gardens and lay a solid foundation for the sericulture industry; implement compound operations to increase the level of income growth; deepen three major innovations,break through the bottlenecks; expand industrial functions,promote integrated development,and accelerate the high quality development of the sericulture industry in Dongtai City.展开更多
Light, heat and water resources in Huicheng Basin had significant advantages, and Huicheng Basin was an ideal area for planting mulberry and breeding silkworm. Climate was mild and humid in the basin, and frost-free p...Light, heat and water resources in Huicheng Basin had significant advantages, and Huicheng Basin was an ideal area for planting mulberry and breeding silkworm. Climate was mild and humid in the basin, and frost-free period was long, which provided favorable condition for planting mulberry and breeding silkworm. Main meteorological disasters affecting mulberry growth included late frost in spring, drought in end spring and early sum- mer. Continuous rain in autumn had certain influence on sericulture, but was favorable for mulberry wintering and growth in opting. At present, carrying capacity of the silkworm in the basin also had large space, and planting mulberry and breeding silkworm had wide developing prospect.展开更多
Tajima Yahei Sericulture Farm,one of the four components of the UNESCO World Heritage Site Tomioka Silk Mill and Related Sites inscribed in 2014,was the starting point for advancements in sericulture farmhouses,emphas...Tajima Yahei Sericulture Farm,one of the four components of the UNESCO World Heritage Site Tomioka Silk Mill and Related Sites inscribed in 2014,was the starting point for advancements in sericulture farmhouses,emphasizing the importance of ventilation.Through Yahei’s experimentation surrounding the ideal space to breed silkworms,a new sericulture theory was invented,and a sericulture farmhouse architecture was formed.For the purpose of preserving this cultural heritage for future generations,it is important to understand the idea that led to the cultural and industrial advancement of the village of Shimamura and to revise the preservation plans prepared by stakeholders.This paper will discuss the development of the sericulture theory,seiryō-iku theory,and the formation of a sericulture farmhouse architecture;describe the adaptation of former sericulture farmhouses and the surrounding landscapes in terms of the industry and environment;outline the plans and efforts for the preservation and revitalization of Tajima Yahei Sericulture Farm,its surrounding area and its underlying issues;and propose a direction for future preservation plans.展开更多
With Guiteyou 2 as the test mulberry variety, mulberry herbaceous cultivation field was established at the planting densities of 60 000,90 000 and 120 000 plants/hm2, respectively. Hybrid mulberry herbaceous cultivati...With Guiteyou 2 as the test mulberry variety, mulberry herbaceous cultivation field was established at the planting densities of 60 000,90 000 and 120 000 plants/hm2, respectively. Hybrid mulberry herbaceous cultivation test was conducted, with Tongxiangqing mulberry field of mid-fist-form at conventional planting density of 4 995 plants/hm2 as the control. The results showed that the leaf yield of herbaceous cultivation mulberry was closely related to the planting density. With the increasing planting density, the leaf yield per plant was decreasing, but the leaf yield per unit area of mulberry field was increasing. The annual leaf yield per unit area in the mulberry field with the average planting density of 120 000 plants/hm2 was 37 560 kg/hm2, 2.14 times of that of the control field. The moisture content of mulberry leaf in herbaceous cultivation field was 4.74% higher than the control; the contents of crude protein, crude fat, crude fiber and crude ash were 1.264%, 0.014%, 0.744% and 0.002%lower than the control, respectively. Comparison of leaf rearing with herbaceous cultivation mulberry and control mulberry showed that there was no significant difference in growth status of silkworm. The cocoon weight, cocoon shell weight, cocoon yield per 10 000 larvae, cocoon filament length and non-broken filament length of Chuanshan × Shushui were 12.81%, 14.29%, 13.85%, 5.95% and 7.68% lower than the control, respectively; but the percentage of common cocoons was 0.16% higher than the control; the cleanness and neatness were 0.2 and 1.25 points higher than the control;the filament size was 0.196 dtex lower than the control. The cocoon weight, cocoon shell weight, cocoon yield per 10 000 larvae, cocoon filament length and non-broken filament length of Jingsong × Haoyue were 11.06%, 10.20%, 11.53%, 9.46% and 9.03% lower than the control, respectively;but the percentage of common cocoons was 1.77% higher than the control; the cleanness and neatness were 0.12 and 2.50 points higher the control;and the filament size was 0.196 dtex lower than the control.展开更多
This study reports the molecular detection of Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworm strains of the Universidade Estadual de Maringá Brazilian Germplasm Bank (UBGB). DNA extraction was carried out b...This study reports the molecular detection of Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworm strains of the Universidade Estadual de Maringá Brazilian Germplasm Bank (UBGB). DNA extraction was carried out by using six Bombyx mori female moths of each strain, followed by PCR amplification. A pair of primers was designed based on a specific sequence of the baculovirus genome related to the BmNPV ORF 14. Another pair of primers was used to amplify the silkworm Actin A3 gene segment, which was used as positive control. Twenty gene pools were analyzed, and fifteen revealed a fragment of 443 base pairs (bp), which indicated the presence of the BmNPV. The frequency of contaminated moths was as following: 100% for silkworm strains M18-2, M12-2 and J1;83% for C25, C75 and C24 strains;66% for KR01;50% for M11-A;33% for AS3, B106, M8 and M11 and 16% for C211, E8 and Hindu strains. These are promising results for the identification of contaminated B. mori moths by BmNPV, which may prevent virus proliferation in subsequent generations. We also analyzed DNA samples extracted from B. mori eggs, but the results were not conclusive regarding the detection of the fragments of the expected size (443 bp). The difficulty in detecting BmNPV contamination in B. mori eggs may be due to the low concentration of virus in samples.展开更多
Mulberry(Morns spp.)is the sole plant consumed by the domesticated silkworm.However,the genome of domesticated mulberry has not yet been sequenced,and the ploidy level of this species remains unclear.Here,we report a ...Mulberry(Morns spp.)is the sole plant consumed by the domesticated silkworm.However,the genome of domesticated mulberry has not yet been sequenced,and the ploidy level of this species remains unclear.Here,we report a high-quality,chromosome-level domesticated mulberry(Morus alba)genome.Analysis of genomic data and karyotype analyses confirmed that M.alba is a diploid with 28 chromosomes(2/7=2x=28).Population genomic analysis based on resequencing of 134 mulberry accessions classified domesticated mulberry into three geographical groups,namely,Taihu Basin of southeastern China(Hu mulberry),northern and southwestern China,and Japan.Hu mulberry had the lowest nucleotide diversity among these accessions and demonstrated obvious signatures of selection associated with environmental adaptation.Further phylogenetic analysis supports a previous proposal that multiple domesticated mulberry accessions previously classified as different species actually belong to one species.This study expands our understanding of genome evolution of the genus Morus and population structure of domesticated mulberry,which would facilitate mulberry breeding and improvement.展开更多
Sericulture is the process of cultivating silkworms for the production of silk.High-quality production of silk without mixing with low quality is a great challenge faced in the silk production centers.One of the possi...Sericulture is the process of cultivating silkworms for the production of silk.High-quality production of silk without mixing with low quality is a great challenge faced in the silk production centers.One of the possibilities to overcome this issue is by separating male and female cocoons before extracting silk fibers from the cocoons as male cocoon silk fibers are finer than females.This study proposes a method for the classification of male and female cocoons with the help of X-ray images without destructing the cocoon.The study used popular single hybrid varieties FC1 and FC2 mulberry silkworm cocoons.The shape features of the pupa are considered for the classification process and were obtained without cutting the cocoon.A novel point interpolation method is used for the computation of the width and height of the cocoon.Different dimensionality reduction methods are employed to enhance the performance of the model.The preprocessed features are fed to the powerful ensemble learning method AdaBoost and used logistic regression as the base learner.This model attained a mean accuracy of 96.3%for FC1 and FC2 in cross-validation and 95.3%in FC1 and 95.1%in FC2 for external validation.展开更多
文摘Silver nanoparticles have mainly been studied for their antimicrobial potential against bacteria, but have also proven to be active against several types of viruses, even against pebrine as well. Methods: Petri dishes and transmission electronic microscope were applied to observe silver nanoparticles against bacteria strains and nuclear polyhedral viruses (NPV) and cytoplasmic polyhedral viruses (CPV). For biological test, 108 CFU/mL NPV with strong pathogenicity, and the NPV samples treated with 1:100 ratio of “Sumerian Silver” for 8 hrs were sprayed on the mulberry leaves, respectively, for silkworm rearing. Conclusion: Silver nanoparticle solution showed a strong bactericidal effect against both G+ and G﹣bacterial pathogenic strains from Bombyx mori and mulberry. Under transmission electronic microscope (TEM), Bacillus sp. Samples showed light particles in the cells when treated with silver nanoparticles, in contrast, untreated samples showed homogeneity contents. Further, NPV particles showed no significant differences between treated and untreated samples, but CPV showed strong effects that almost all CPV were collapsed. For biological test, “Sumerian Silver” treated NPV showed no diseased silkworm but nearly all silkworms were dead with no treatment. It seems like that silver nanoparticles were proved to be more effective against CPV than that of NPV. The significant differences between two antivirus or virucidal mechanism should be greatly aroused the scientific interest.
文摘With many years of benign development,Dongtai City has established a complete technical system for the sericulture industry,an advanced industrialized operation mode,a mature operation mechanism of trade,industry and agriculture,and a perfect social service mode.The production level of sericulture in Dongtai City is constantly improved. However,there are still problems such as small scale of individual household,slow technological progress,low level of industrial mechanization and labor saving,and hidden dangers in safe production of sericulture. It is recommended to fully build mulberry gardens and lay a solid foundation for the sericulture industry; implement compound operations to increase the level of income growth; deepen three major innovations,break through the bottlenecks; expand industrial functions,promote integrated development,and accelerate the high quality development of the sericulture industry in Dongtai City.
文摘Light, heat and water resources in Huicheng Basin had significant advantages, and Huicheng Basin was an ideal area for planting mulberry and breeding silkworm. Climate was mild and humid in the basin, and frost-free period was long, which provided favorable condition for planting mulberry and breeding silkworm. Main meteorological disasters affecting mulberry growth included late frost in spring, drought in end spring and early sum- mer. Continuous rain in autumn had certain influence on sericulture, but was favorable for mulberry wintering and growth in opting. At present, carrying capacity of the silkworm in the basin also had large space, and planting mulberry and breeding silkworm had wide developing prospect.
文摘Tajima Yahei Sericulture Farm,one of the four components of the UNESCO World Heritage Site Tomioka Silk Mill and Related Sites inscribed in 2014,was the starting point for advancements in sericulture farmhouses,emphasizing the importance of ventilation.Through Yahei’s experimentation surrounding the ideal space to breed silkworms,a new sericulture theory was invented,and a sericulture farmhouse architecture was formed.For the purpose of preserving this cultural heritage for future generations,it is important to understand the idea that led to the cultural and industrial advancement of the village of Shimamura and to revise the preservation plans prepared by stakeholders.This paper will discuss the development of the sericulture theory,seiryō-iku theory,and the formation of a sericulture farmhouse architecture;describe the adaptation of former sericulture farmhouses and the surrounding landscapes in terms of the industry and environment;outline the plans and efforts for the preservation and revitalization of Tajima Yahei Sericulture Farm,its surrounding area and its underlying issues;and propose a direction for future preservation plans.
基金Supported by Technology Research and Development Program of Nanchong,2015(No.15A0053)
文摘With Guiteyou 2 as the test mulberry variety, mulberry herbaceous cultivation field was established at the planting densities of 60 000,90 000 and 120 000 plants/hm2, respectively. Hybrid mulberry herbaceous cultivation test was conducted, with Tongxiangqing mulberry field of mid-fist-form at conventional planting density of 4 995 plants/hm2 as the control. The results showed that the leaf yield of herbaceous cultivation mulberry was closely related to the planting density. With the increasing planting density, the leaf yield per plant was decreasing, but the leaf yield per unit area of mulberry field was increasing. The annual leaf yield per unit area in the mulberry field with the average planting density of 120 000 plants/hm2 was 37 560 kg/hm2, 2.14 times of that of the control field. The moisture content of mulberry leaf in herbaceous cultivation field was 4.74% higher than the control; the contents of crude protein, crude fat, crude fiber and crude ash were 1.264%, 0.014%, 0.744% and 0.002%lower than the control, respectively. Comparison of leaf rearing with herbaceous cultivation mulberry and control mulberry showed that there was no significant difference in growth status of silkworm. The cocoon weight, cocoon shell weight, cocoon yield per 10 000 larvae, cocoon filament length and non-broken filament length of Chuanshan × Shushui were 12.81%, 14.29%, 13.85%, 5.95% and 7.68% lower than the control, respectively; but the percentage of common cocoons was 0.16% higher than the control; the cleanness and neatness were 0.2 and 1.25 points higher than the control;the filament size was 0.196 dtex lower than the control. The cocoon weight, cocoon shell weight, cocoon yield per 10 000 larvae, cocoon filament length and non-broken filament length of Jingsong × Haoyue were 11.06%, 10.20%, 11.53%, 9.46% and 9.03% lower than the control, respectively;but the percentage of common cocoons was 1.77% higher than the control; the cleanness and neatness were 0.12 and 2.50 points higher the control;and the filament size was 0.196 dtex lower than the control.
基金supported by CAPES,CNPq,FINEP/Fundacao Araucaria and Secretaria de Estado da Ciencia,Tecnologia e Ensino Superior—FUNDO PARANA.
文摘This study reports the molecular detection of Bombyx mori nucleopolyhedrovirus (BmNPV) in silkworm strains of the Universidade Estadual de Maringá Brazilian Germplasm Bank (UBGB). DNA extraction was carried out by using six Bombyx mori female moths of each strain, followed by PCR amplification. A pair of primers was designed based on a specific sequence of the baculovirus genome related to the BmNPV ORF 14. Another pair of primers was used to amplify the silkworm Actin A3 gene segment, which was used as positive control. Twenty gene pools were analyzed, and fifteen revealed a fragment of 443 base pairs (bp), which indicated the presence of the BmNPV. The frequency of contaminated moths was as following: 100% for silkworm strains M18-2, M12-2 and J1;83% for C25, C75 and C24 strains;66% for KR01;50% for M11-A;33% for AS3, B106, M8 and M11 and 16% for C211, E8 and Hindu strains. These are promising results for the identification of contaminated B. mori moths by BmNPV, which may prevent virus proliferation in subsequent generations. We also analyzed DNA samples extracted from B. mori eggs, but the results were not conclusive regarding the detection of the fragments of the expected size (443 bp). The difficulty in detecting BmNPV contamination in B. mori eggs may be due to the low concentration of virus in samples.
基金The work was supported by the National Key Research and Development Project of China,China(no.2019YFD1000600)the Fundamental Research Funds for the Central Universities of Northwest A&F University,China(2452619041)Funds of Modern Agricultural Industrial Technology System(no.CARS-18).
文摘Mulberry(Morns spp.)is the sole plant consumed by the domesticated silkworm.However,the genome of domesticated mulberry has not yet been sequenced,and the ploidy level of this species remains unclear.Here,we report a high-quality,chromosome-level domesticated mulberry(Morus alba)genome.Analysis of genomic data and karyotype analyses confirmed that M.alba is a diploid with 28 chromosomes(2/7=2x=28).Population genomic analysis based on resequencing of 134 mulberry accessions classified domesticated mulberry into three geographical groups,namely,Taihu Basin of southeastern China(Hu mulberry),northern and southwestern China,and Japan.Hu mulberry had the lowest nucleotide diversity among these accessions and demonstrated obvious signatures of selection associated with environmental adaptation.Further phylogenetic analysis supports a previous proposal that multiple domesticated mulberry accessions previously classified as different species actually belong to one species.This study expands our understanding of genome evolution of the genus Morus and population structure of domesticated mulberry,which would facilitate mulberry breeding and improvement.
基金funded by the Department of Science&Technology(DST)with grant reference number:SEED/WS/2019/135.
文摘Sericulture is the process of cultivating silkworms for the production of silk.High-quality production of silk without mixing with low quality is a great challenge faced in the silk production centers.One of the possibilities to overcome this issue is by separating male and female cocoons before extracting silk fibers from the cocoons as male cocoon silk fibers are finer than females.This study proposes a method for the classification of male and female cocoons with the help of X-ray images without destructing the cocoon.The study used popular single hybrid varieties FC1 and FC2 mulberry silkworm cocoons.The shape features of the pupa are considered for the classification process and were obtained without cutting the cocoon.A novel point interpolation method is used for the computation of the width and height of the cocoon.Different dimensionality reduction methods are employed to enhance the performance of the model.The preprocessed features are fed to the powerful ensemble learning method AdaBoost and used logistic regression as the base learner.This model attained a mean accuracy of 96.3%for FC1 and FC2 in cross-validation and 95.3%in FC1 and 95.1%in FC2 for external validation.
