[Objective]The paper was to study the effects of fermentation bed padding on intestinal flora,serum biochemical indexes and immune function of growing-finishing pigs.[Method]A total of 90 growing-finishing pigs(Duroc ...[Objective]The paper was to study the effects of fermentation bed padding on intestinal flora,serum biochemical indexes and immune function of growing-finishing pigs.[Method]A total of 90 growing-finishing pigs(Duroc × Landrace × Yorkshire)with the body weight of(30.5±0.8)kg were randomly divided into five groups,three replicates each group,six pigs each replicate.The padding of five groups were:50%rice husk+50%sawdust(control group),100%spent mushroom substrate of Pleurotus eryngii(100%spent mushroom substrate group),15%rice husk+15%sawdust+70%spent mushroom substrate(70%spent mushroom substrate group),25%rice husk+25%sawdust+50%spent mushroom substrate(50%spent mushroom substrate group),35%rice husk+35%sawdust+30%spent mushroom substrate(30%spent mushroom substrate group).The pre-trial lasted 7 d,and the formal trial lasted 90 d.[Result]Compared with the control group,the number of Escherichia col in 100%,70%and 50%spent mushroom substrate groups decreased by 35.4%,24.0%and 20.8%,respectively(P<0.05);the number of salmonella successively decreased by 29.4%,19.3%and 15.2%,respectively(P<0.05);the number of lactobacillus successively increased by 21.7%,15.9%and 12.2%,respectively(P<0.05);and the number of bifidobacteria successively increased by 20.2%,16.7%and 11.8%,respectively(P<0.05).Compared with the control group,the total serum protein content in 100%,70%,50%and 30%spent mushroom substrate groups increased by 30.6%,33.0%,28.0%and 18.4%,respectively(P<0.05);and the serum urea nitrogen content decreased by 32.4%,34.5%,33.9%and 22.0%,respectively(P<0.05).There was no significant difference in serum albumin,aspartate aminotransferase(AST)and alanine aminotransferase(ALT)between treatment groups and control group.The IgA,IgG,IgM and IL-6 levels in serum of each treatment group were significantly different from those of the control group(P<0.05).There was no significant difference in IgA,IgG,IgM and IL-6 levels among 100%,70%and 50%spent mushroom substrate groups.[Conclusion]It improves the balance of intestinal microecological flora of growing-finishing pigs and enhances the immune performance of pigs by using spent mushroom substrate of P.eryngii as the fermentation bed padding.15%rice husk+15%sawdust+70%spent mushroom substrate is the optimal proportion of spent mushroom substrate for growing-finishing pigs.展开更多
To evaluate the acute lung toxicity and the effect on serum biochemical indexes of inhaled TiO2 nanotubes , healthy and adult Kunming mice are exposed to aerosols of TiO2nanotubes in a sealed chamber , the concentrati...To evaluate the acute lung toxicity and the effect on serum biochemical indexes of inhaled TiO2 nanotubes , healthy and adult Kunming mice are exposed to aerosols of TiO2nanotubes in a sealed chamber , the concentration of which is 250mg / m 3 while another group of mice are exposed to room air only served as control.The blood , alveolar lavaged fluid and lungs of the mice are collected and examined after exposed for 7 , 14and 28d , respectively.The serum analysis shows that glucose ( Glu ) values with 7dexposure , alanine aminotransferase ( ALT ) and aspartate aminotransferase ( AST ) activities with 28dinhalation , and total bile acid ( TBA ) values with 7 , 28d as well as the creatine kinase ( CK ) levels with 28dexposure are significantly different from those of controls ( P< 0.05 ) .The pathological findings exhibit that more TiO 2 nanotubes are found in the interstitium of pulmonary alveoli with the experimental time prolonged.The results suggest that TiO 2 nanotubes do produce adverse responses to the lung and serum biochemical indexes of mice.Moreover , the responses become severer with the exposed-time prolonged.展开更多
[Objective] To investigate the effects of traditional Chinese medicine compound preparation on serum biochemical indexes and egg nutritional quality of laying hens. [Method] A total of 1 200 chickens at the age of 44 ...[Objective] To investigate the effects of traditional Chinese medicine compound preparation on serum biochemical indexes and egg nutritional quality of laying hens. [Method] A total of 1 200 chickens at the age of 44 weeks were selected and randomly divided into five groups. After administration with different dosages of astragalus polysaccharides and berberine for 9 weeks,serum biochemical indexes were determined and nutrient contents of egg were detected. [Result] The contents of total protein,globulin,high-density lipoprotein cholesterol(HDL-C) and alkaline phosphatase(ALP) in all treatment groups were significantly(P < 0.05 or 0.01) higher than those in the control group. The cholesterol contents of all treatment groups were significantly(P < 0.05 or 0.01) lower than those of the control group. [Conclusion] The optimal concentrations of astragalus polysaccharides and berberine in basic diets are 200 and 30 mg/kg,respectively.展开更多
[Objective]This experiment was conducted to study the effects of dietary betaine on performance,egg quality and serum biochemical parameters of laying hens under heat stress condition. [Method]A total of 600 22-week-o...[Objective]This experiment was conducted to study the effects of dietary betaine on performance,egg quality and serum biochemical parameters of laying hens under heat stress condition. [Method]A total of 600 22-week-old commercial Roman laying hens were randomly divided into five groups,with eight replicates in each group and 15 hens in each replicate. Group I was taken as the positive control group,and laying hens in group I were fed with basal diets in normal thermal environment. Temperature humidity index of group I was between 64. 9 and 68. 9. Group II was taken as the negative control group,and laying hens in group II were fed with basal diets under heat stress condition. Temperature humidity index of group II was higher than 72. Laying hens in groups III-V were fed with basal diets supplemented with 200,400 and 600 mg/kg betaine under heat stress condition respectively,and temperature humidity indexes in groups III-V were all higher than 72. The experiment lasted for 14 weeks. [Results]Results showed that there were no significant differences in average daily feed intake,feed egg ratio and broken egg rate among different test groups( P > 0. 05). Hen-housed laying rate,hen-housed egg yield,serum total protein( TP) content and serum alkaline phosphatase( AKP) activity in group II were significantly lower than those in group I( P < 0. 05). However,activities of glutamic-oxalacetic transaminase( GOT),creatine kinase( CK) and glutamic-pyruvic transaminase( GPT) in group II were significantly higher than those in group I( P < 0. 05). Hen-housed laying rate,hen-housed egg yield and serum TP content in group IV were significantly higher than those in group II( P < 0. 05). Hen-housed egg yield,serum TP content and serum albumin( ALB) content in group V were significantly higher than those in group II( P < 0. 05). Activities of serum CK and GPT in groups IV and V were significantly lower than those in group II( P < 0. 05),serum triglyceride( TG) content in group V was significantly lower than that in group II( P < 0. 05).[Conclusion]Metabolism and physiological function of laying hens could be affected by heat stress,and which caused a decline in performance. Adding of dietary betaine in diets could improve hen-housed egg production and hen-housed laying rate,which could also improve the health of laying hens under heat stress condition. The appropriate dietary adding content of betaine was 400 mg/kg.展开更多
[Objective] This trial was to investigate the effect of dietary Picria felterrae Lour extracts on serum biochemical indexes and immune function of Guangxi Partridge chickens. [Method] Totally 500 individuals of 1-day-...[Objective] This trial was to investigate the effect of dietary Picria felterrae Lour extracts on serum biochemical indexes and immune function of Guangxi Partridge chickens. [Method] Totally 500 individuals of 1-day-old Guangxi Partridge chickens were randomly divided into five groups, five replicates each group and 20 chicks each replicate. Groups A, B and C were treatment groups supplemented with 0.70%, 0.35% and0.175% P. felterrae extracts in the basal diet, respectively; group D was a medical control group supplemented with 0.01% colistin sulfate premix in the basal diet; and group E was a control group with basal diet. Serum samples were collected from 10 chickens in each group at 21, 35 and 49 days of age, to analyze the biochemical and immune indexes. [Result] It had no significant impact on serum TP and GLU contents of chickens by adding different levels of P. felterrae extracts in the basal diet(P >0.05). However, at 21 and 35 days of age, the TBILI content of chickens in groups A, B and C were extremely lower or significantly lower than that in group E( P<0.01; P<0.05). At 35 days of age, the serum AKP activities in groups A, B and C were extremely higher than that in group E(P<0.01), and the serum GOT activities at 21 and 35 days of age in group C were significantly higher than that in group E(P<0.05). At 49 days of age, the serum Ig G content in groups A, B and C and the IL-2 content in group B and group C were significantly lower or extremely lower than that in group E( P<0.05; P<0.01); the T-AOC content in group B was significantly higher than that in group E(P<0.05); the GSH content in groups A, B and C were significantly higher than that in group E( P<0.05). Adding different levels of P. felterrae extracts in the diet significantly decreased the TBILI content, reduced the Ig G, IL-2 and IL-6 contents, and improved the AKP and GOT activities and the T-AOC and GSH contents of Guangxi Partridge chicken. [Conclusion] Adding 0.35% P. felterrae extracts in the basal diet received the best effort.展开更多
This study investigated the effects of protein sources for milk replacers on growth performance and serum biochemical indexes of suckling calves. Fifty Chinese Holstein bull calves with similar BW and age were randoml...This study investigated the effects of protein sources for milk replacers on growth performance and serum biochemical indexes of suckling calves. Fifty Chinese Holstein bull calves with similar BW and age were randomly allocated to 5 groups(1 control and 4 treatments) of 10 calves in each group. Five types of milk replacers were designed to have the same level of energy and protein. The protein source for milk replacers of the control group was full milk protein(MP). The protein source of milk replacers of the 4 treatment groups was composed of MP and one vegetable protein(VP)(30 and 70% of total protein). The4 types of VP were soybean protein concentrate(SP), hydrolyzed wheat protein(WP), peanut protein concentrate(PP), and rice protein isolate(RP). Results of the experiment showed: 1) there was no significant difference on average daily gain(ADG) and feed:gain ratio(F:G) among the MP, SP and RP groups(P > 0.05), whereas the ADG and F:G of the WP and PP groups were significantly lower compared with the MP group(P < 0.05); 2) there was not a significant difference in withers height, body length and heart girth among treatment groups compared with the MP group(P > 0.05). Thereby the 4 VP milk replacers had no adverse effects on body size of calves; 3) all groups showed no significant difference in the serum contents of urea nitrogen, total protein, albumin, globulin, β-hydroxybutyrate, growth hormone, insulin-like growth factor-1, and the ratio of albumin to globulin(A:G)(P> 0.05). In conclusion, SP or RP(accounts for 70% of the total protein) as calf milk replacers could substitute MP, whereas wheat gluten and PP had a significant adverse effect on growth performance in this experiment.展开更多
[Objective] The study was carried out to investigate the effects of dietary crude fiber on growth performance and serum biochemical in-dex of Dingan geese aged from 35 to 70 days.[Method] A total of 120 Dingan geese o...[Objective] The study was carried out to investigate the effects of dietary crude fiber on growth performance and serum biochemical in-dex of Dingan geese aged from 35 to 70 days.[Method] A total of 120 Dingan geese on day 28 with the same genetic background and similar initial weight(1 384.83.73±0.76 g) were randomly assigned into 3 groups, with 4 replicates per group and 10 geese per replicate. Geese were fed the diets containing 4.29%, 5.29% and 6.29 % crude fiber, respectively. The experiment lasted 35 d.[Result] Final weight and daily gain of 5.29 %CF group were significantly higher than those of 6.29 % CF group(P<0.05);Feed intake was markedly higher than those of 4.29% and 6.29 % CF groups(P<0.05);Feed gain ratio was significantly lower than that of 6.29 % CF group(P<0.05). Compared with 4.29% and 6.29 % CF groups, serum albumin concentrations of 5.29 % CF group increased significantly(P<0.05), while serum total protein and globin contents were higher(P>0.05), but serum uric acid level was lower(P>0.05).[Conclusion] 5.29 % crude fiber could lead to higher growth performance of Dingan geese.展开更多
[Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with ...[Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with the same genetic backgrounds and similar initial weight of(10.05±0.049) kg were randomly assigned into two treatments, and supplied with Juncao + concentrate(control group), pelleted total mixed ration(experimental group), respectively. The trial lasted 50 d.[Result] The final weight, daily gain and feed intake of experimental group were significantly higher than those of control group(P<0.05), but the feed/gain ratio of experimental group was markedly lower than that of control group(P<0.05). Compared with the control group, the gross profit of experimental group significantly increased( P <0.05).[Conclusion] Pelleted total mixed ration led to better production performance of growing Hainan black goat.展开更多
Background:Sows are frequently subjected to various stresses during late gestation and lactation,which trigger inflammatory response and metabolic disorders.Dietary fiber can influence animal health by modulating gut ...Background:Sows are frequently subjected to various stresses during late gestation and lactation,which trigger inflammatory response and metabolic disorders.Dietary fiber can influence animal health by modulating gut microbiota and their by-products,with the effects depending upon the source of the dietary fiber.This study aimed to evaluate the impacts of different fiber sources on body condition,serum biochemical parameters,inflammatory responses and fecal microbiota in sows from late gestation to lactation.Methods:Forty-five multiparous sows(Yorkshire×Landrace;3–6 parity)were assigned to 1 of 3 dietary treatments from d 85 of gestation to the end of lactation(d 21 post-farrowing):a control diet(CON,a corn-soybean meal diet),a sugar beet pulp diet(SBP,20%SBP during gestation and 10%SBP during lactation),and a wheat bran diet(WB,30%WB during gestation and 15%WB during lactation).Results:Compared with CON,supplementation of SBP decreased(P<0.05)lactation BW loss,reduced(P<0.05)serum concentration of total cholesterol,non-esterified fatty acids,interleukin-6 and tumor necrosis factor-α,and increased(P<0.05)fecal water content on d 110 of gestation and d 21 of lactation,while supplementation of WB reduced(P<0.05)serum concentration of total cholesterol on d 110 of gestation,increased(P<0.05)fecal water content and decreased(P<0.05)serum interleukin-6 concentration on d 110 of gestation and d 21 of lactation.In addition,sows fed SBP had lower(P<0.01)abundance of Clostridium_sensu_stricto_1 and Terrisporobacter than those fed CON,but had greater(P<0.05)abundance of Christensenellaceae_R-7_group and Ruminococcaceae_UCG-002 than those fed the other two diets on d 110 of gestation.On d 21 of lactation,supplementation of SBP decreased(P<0.05)the abundance of Firmicutes and Lactobacillus,but enriched(P<0.05)the abundance of Christensenellaceae_R-7_group,Prevotellaceae_NK3B31_group,Ruminococcaceae_UCG-002,Prevotellaceae_UCG_001 and unclassified_f__Lachnospiraceae compared with WB.Compared with CON,sows fed SBP had greater(P<0.05)fecal concentrations of acetate,butyrate and total SCFAs during gestation and lactation,while sows fed WB only had greater(P<0.05)fecal concentration of butyrate during lactation.Conclusions:Supplementation of dietary fiber during late gestation and lactation could improve sow metabolism and gut health,and SBP was more effective than WB.展开更多
This experiment was conducted to evaluate the effect of onion (Allium cepa L.) as an antibiotic growth promoter substitute on growth performance, immune responses and serum biochemistry in broilers. A total of 192 one...This experiment was conducted to evaluate the effect of onion (Allium cepa L.) as an antibiotic growth promoter substitute on growth performance, immune responses and serum biochemistry in broilers. A total of 192 one-d-old as hatched broiler chicks (Ross 308) were weighed and randomly allocated to four treatment groups, each with 4 replicate pens of 12 chicks. The dietary treatments consisted of the basal diet (control), antibiotic (15 mg Virginiamycin/kg), and control +10 or 30 g fresh onion bulb/kg diet. Body weights of broilers were determined at d 1, 21 and 42, feed intake was determined at the same periods, and feed conversion ratio was calculated accordingly. At 14th and 21st days blood samples were taken for measuring antibody titers against NDV and at 42nd day for biochemical analysis. At d 42, two birds per replicate were slaughtered for determination of lymphoid organ weights. Dietary supplementation of30 g/kg onion increased final body weight of broilers at 42nd d of age compared to the other treatments (P < 0.05). Birds fed30 gonion/kg in the diet had the highest feed intake than other treatments at different growth periods (P < 0.05). Dietary treatments failed to induce any significant effect on antibody titers against NDV, although the weight of lymphoid organs was significantly (P Broilers receiving30 g/kg onion had a significantly higher HDL and lower triglyceride concentrations compared to control groups (P . Feeding30 g/kg onion resulted in a marked reduction in the concentration of the glucose compared to control groups (P . The results suggested that dietary inclusion of30 g/kg onion can be applied as alternatives to in-feed antibiotics for broiler diets.展开更多
[Objective] The paper to investigate the effects of pelleted total mixed ration of different particle size on production performance and serum biochemical index of fattening Hainan black goats.[Method] Thirty-six fatt...[Objective] The paper to investigate the effects of pelleted total mixed ration of different particle size on production performance and serum biochemical index of fattening Hainan black goats.[Method] Thirty-six fattening Hainan black goats with the same genetic backgrounds and similar initial weight of(19.02±0.22) kg were randomly divided into three treatments. Goats were fed with Juncao+concentrate(control group), pel-leted total mixed ration I(particle size 6 mm, experimental group I) and II(particle size 5 mm, experimental group II), respectively. The trial lasted 45 d.[Result](1) The final weight and daily gain of experimental group II were significantly higher than those of control group( P<0.05), and the daily gain of experimental group II was also higher than that of experimental group I(P<0.05);the feed intake of experimental group II was markedly higher than that of control group(P<0.05), but was significantly lower than that of experimental group I(P<0.05). The feed/gain ratio of experimental group II was significantly lower than those of control group and experimental group I(P<0.05).(2) The feed gain cost of experimental group II was markedly lower than that of control group(P<0.05), but the gross profit was significantly higher than those of control group and experimental group I(P<0.05).(3) Compared with the control group, the serum total protein content of experimental group II increased markedly( P<0.05) while the urea nitrogen level decreased(P>0.05).[Conclusion] The pelleted total mixed ration with the particle size of 5 mm obtained better fattening ef-fect, and obviously improved the serum total protein level.展开更多
[Objective]The paper was to investigate the effects of dietary crude fiber level on production performance,organ index and serum biochemical parameters of Dingan geese aged from 1 to 28 days.[Method]A total of 144 1-d...[Objective]The paper was to investigate the effects of dietary crude fiber level on production performance,organ index and serum biochemical parameters of Dingan geese aged from 1 to 28 days.[Method]A total of 144 1-day-old Dingan geese with the same genetic background and similar initial weight(88.75±0.21g)were randomly assigned into 3 groups with 4 replicates per group and 12 geese per replicate,and fed with the diets containing 2.56%,3.52% and 4.48% crude fiber,respectively.The trial lasted 28 d.[Result]1)The final weight and daily gain of 3.52% CF group were significantly higher than those of 2.56% CF group(P<0.05);the feed/gain ratio was markedly lower than that of 2.56% CF group(P<0.05).2)The slaughter rate,semi-eviscerated rate and leg muscle rate of 3.52% CF group were higher than those of 2.56% and 4.48% CF groups(P>0.05).3)As the dietary crude fiber levels rose,the abdominal fat rate showed a downward trend(P>0.05).4)The liver index of 3.52% CF group was significantly higher than that of 2.56% CF group(P<0.05).4)Compared with 2.5%and 4.48% CF groups,the serum albumin concentrations of 3.52% CF group markedly increased(P<0.05),the serum total protein and globin contents were higher(P>0.05),but the serum uric acid level was lower(P>0.05).[Conclusion]When the crude fiber level was 3.52%,the production performances of Dingan geese aged from 1 to 28 days were better,and the liver index and serum albumin level could be obviously improved.展开更多
In order to provide reference for using Siganus guttatus to control overgrowth of Enteromorpha prolifra,the growth,serum biochemical and antioxidant enzyme indices of juvenile S.guttatus respectively fed with E.prolif...In order to provide reference for using Siganus guttatus to control overgrowth of Enteromorpha prolifra,the growth,serum biochemical and antioxidant enzyme indices of juvenile S.guttatus respectively fed with E.prolifra and artificial feed were studied.One hundred and eighty individuals were cultivated for 90 days in six 2.5 m × 1.5 m × 1.5 m cages (30 ind.per cage) which mesh size were 0.5 mm.The experimental animals were divided into two dietary groups (three cages for each group) that were fed with E.prolifra and artificial feed respectively.During the culture period,temperatures ranged from 23.0 to 26.5℃,p H was between 7.8 and 8.2,dissolved oxygen was more than 5.0 mg/L.The weight gain rate,specific growth rate,relative growth rate,hepato-somatic index of juvenile S.guttatus fed with E.prolifra were significantly lowe than that fed with artificial feed (P<0.05).The total protein (TP),urea nitrogen (UN)and alkaline phosphatase (AKP) of juvenile S.guttatus fed with E.Prolifra were significantly higher than that fed with artificial feed (P<0.05),while glutamic oxalacetic transaminase (AST/GOT) and glutamic-pyruvic transaminase (ALT/GPT) of juvenile S.guttatus fed with E.prolifra were significantly lower than that fed with artificial feed (P<0.05).SOD,GPX and anti-superoxide anion radical activity in liver,kidney,gill and muscle of juvenile S.guttatus fed with E.prolifra were higher than that fed with artificial feed,and these antioxidant enzyme activities in kidney of juvenile S.guttatus fed with E.prolifra was significantly higher than that fed with artificial feed (P<0.05),while CAT and hydroxyl radical-inhibiting activity in liver of juvenile S.guttatus fed with artificial feed were significantly higher than that fed with E.prolifra (P<0.05).Juvenile S.guttatus fed with E.prolifra showed poor growth performance but better antioxidant defense system.S.guttatus is easily raised and has stronger ability of digestion and absorption of E.Prolifra.The technique that uses S.guttatus to control E.prolifra deserves deeply study.展开更多
[Objective]The paper was to investigate the effects of dietary concentrate to forage ratios on production performance and serum bio-chemical indicators of post-fattening Hainan Yellow Cattle.[Method]Sixteen Hainan Yel...[Objective]The paper was to investigate the effects of dietary concentrate to forage ratios on production performance and serum bio-chemical indicators of post-fattening Hainan Yellow Cattle.[Method]Sixteen Hainan Yellow Cattles with the same genetic backgrounds and similar initial weight of(242.22 ±0.71 kg) were randomly divided into four treatments.Cattles were fed with diets of different concentrate to forage ratios(20:80, group Ⅰ;30:70, Group Ⅱ;40:60, group Ⅲ;50:50, group Ⅳ).The trial lasted 62 d.[Result]The daily gain in experimental group Ⅳ was significantly higher than that in experimental group Ⅱ(P<0.05), but the feed gain ratio in experimental group Ⅳ was lower than those in other ex-perimental groups(P>0.05).The feed intake in experimental group Ⅳ was also markedly higher than those in experimental groups Ⅰ and Ⅱ(P<0.05).Compared with the experimental group Ⅱ, the gross profit of experimental group Ⅳ was significantly promoted(P<0.05).The alanine aminotransferase and aspartate aminotransferase activities in experimental group Ⅱ were markedly higher than those in experimental groups Ⅰ, Ⅲ and Ⅳ.[Conclusion]When the dietary concentrate to forage ratios was 50∶50, the production performance of post-fattening Hainan Yellow Cattle was better.展开更多
Purpose: We evaluated biochemical analysis results with the aim of discovering serum levels that have possible effects on the development of retinopathy of prematurity (ROP). Methods: A retrospective study was conduct...Purpose: We evaluated biochemical analysis results with the aim of discovering serum levels that have possible effects on the development of retinopathy of prematurity (ROP). Methods: A retrospective study was conducted between January 2017 and January 2018 on a total of 110 infants with 35 or less gestational weeks. The sample included 78 infants who had been diagnosed with different stages of ROP and 32 infants without ROP. Results from routine serum biochemical analyses, performed at birth and at one month after birth, were evaluated. The Independent Sample t-test and Mann-Whitney U test were performed to compare the data. Results: The infants with ROP were born at a mean of 28.0 (±2.1) weeks of gestation, weighing a mean of 1066 (±314) g, and the mean duration of stay in the incubator was 38.2 (±19) days. The infants without ROP were born at a mean of 29.6 (±2.6) weeks of gestation, weighing a mean of 1265 (±372) g. 59 infants (53.6%) were females, and 51 (46.4%) were males. Between infants with and without ROP, differences in the following levels were not statistically significant: glucose, blood urine nitrogen, creatinine, alanine aminotransferase, aspartate aminotransferase, albumin, total protein, direct bilirubin, indirect bilirubin, uric acid, and phosphorus. Serum magnesium (Mg) levels at birth in infants with ROP were significantly higher than infants without ROP (p = 0.014). Conclusions: Serum Mg levels at birth were found to be higher in infants with ROP than in those without ROP. The levels may be dependent on the mothers’ treatment of Mg for different medical reasons.展开更多
The objective of this study was to investigate the effects of phytase supplementation on growth performance, slaughter performance, growth of internal organs and small intestine, and serum biochemical indices of broil...The objective of this study was to investigate the effects of phytase supplementation on growth performance, slaughter performance, growth of internal organs and small intestine, and serum biochemical indices of broilers. A total of 360 1-day-old Ross 308 broilers were randomly divided into 4 groups with 3 replicates and 30 broilers per replicate (15 male, 15 female). The treatments were fed with basal diet supplemented with 0%, 0.01%, 0.02% and 0.03% phytase. The results showed that: 1) Phytase supplementation increased the body weight gain and the body weight of Ross 308 broilers (P serum calcium (Ca) consistency was 0.02%. According to the above analysis, the feasible supplementation of phytase (enzyme activity for 5000 u/g) in broiler dietary was 0.02%.展开更多
In order to study the effects of replacing different proportions of silage maize with silage sweet sorghum treated by different fermentation methods on the production performance and blood biochemical indexes of dairy...In order to study the effects of replacing different proportions of silage maize with silage sweet sorghum treated by different fermentation methods on the production performance and blood biochemical indexes of dairy cows,25 Chinese Holstein lactating cows were randomly divided into 5 groups,5 in each group.The control check (CK) was fed the basal diet;for the experimental group 1 and the experimental group 2,50% of the silage maize in the basal diet was replaced with the additive silage sweet sorghum and the conventional silage sweet sorghum,respectively;and as to the experimental group 3 and the experimental group 4,all the silage maize in the basal diet was replaced with additive silage sweet sorghum and conventional silage sweet sorghum,respectively.The preliminary trial period was 7 d,and the trial period was 35 d.The results showed that the experimental group 1 and the experimental group 3 had the dry matter intake significantly higher than that of the experimental group 2,the experimental group 4 and the CK ( P <0.05).The daily milk yields of the experimental group 1 and the experimental group 2 were significantly lower than that of the CK ( P <0.05),and the experimental group 3 and the experimental group 4 were significantly lower than the experimental group 1 and the experimental group 2 ( P <0.05).The milk protein percentage of the experimental group 2 was extremely significantly higher than that of the experimental group 4 ( P <0.01).The experimental group 1,the experimental group 2 and the experimental group 3 showed the blood glucose levels extremely significantly higher than that in the CK ( P <0.01).The blood urea nitrogen contents in the experimental group 2 and the experimental group 4 were significantly higher than those in the experimental group 1 and the CK ( P <0.01).There were no significant differences in other blood biochemical indexes between various groups ( P >0.05).It is feasible to use silage sweet sorghum to feed dairy cows,but the proportion should not be too large,and attention should be paid to the energy and nitrogen balance of the diet.展开更多
To investigate the effects of dietary supplementation with hydrolyzed wheat gluten (HWG) on growth performance, cell immunity and serum biochemical indices of weaned piglets, 160 crossed (Large White×andrace) and...To investigate the effects of dietary supplementation with hydrolyzed wheat gluten (HWG) on growth performance, cell immunity and serum biochemical indices of weaned piglets, 160 crossed (Large White×andrace) and weaned piglets were randomly divided into 4 treatments with 4 replicates of 10 piglets each. The piglets in each treatment were fed an experimental diet containing either 0 g kg-1 HWG (control group), 30 g kg-1 HWG (3% HWG group), 50 g kg-1 HWG (5% HWG group), or 2.5 g kg-1 glycyl-L-glutamine (0.25% Gly-Gln group). The results showed that the diarrhea rates in 3% HWG and 5% HWG groups were significantly lower than in control group from d 1 to 14 (P<0.05), while the average daily gain (ADG) in each of two groups was increased (P>0.05). When compared with control group, dietary supplementation with 3% HWG increased the ratio of CD4+:CD8+ and the ratio of serum albumin and globulin concentrations (A:G) on d 14 and 28, as well as the proliferation of T- and B-lymphocytes (P>0.05) on d 28. In addition, on d 14 and 28, the A:G ratio in 5% HWG group was significantly higher than in control group (P<0.05), while the ratio of CD4+:CD8+ increased slightly (P>0.05). Interestingly, 0.25% Gly-Gln group had higher proportion of CD3+ (P>0.05) and CD4+ (P<0.05) on d 14 than control group, but growth performances of 0.25% Gly-Gln group were negatively affected at all experiment stages. These results suggested that HWG might improve the growth performance of piglets by strengthening cell immunity and decreasing the occurrence of diarrhea during the prophase after weaning.展开更多
The influence of exercise at high temperature on adult males' routine blood indexes and biochemical indexes and the expression of HSP72 in peripheral blood lymphocytes (PBLs) was studied in order to provide theore...The influence of exercise at high temperature on adult males' routine blood indexes and biochemical indexes and the expression of HSP72 in peripheral blood lymphocytes (PBLs) was studied in order to provide theoretical ground for health supervision of adults receiving exercise at high temperature. 180 adult males were selected and divided into exercise group and control group, in which the exercise group was subdivided into subgroup 1 and subgroup 2 receiving exercise at high temperature in the afternoon and in the morning, respectively. Peripheral venous blood was phle- botomized before and after the exercise to examine routine blood indexes and blood biochemical indexes. The expression levels of HSP72 in PBLs were detected by flow cytometry. The results showed that the routine blood indexes and biochemical indexes in each group were within the range of normal values of male adults. There was no significant difference between each exercise group and control group in indexes before exercise. After exercise, the expression levels of HSP72 in PBLs in exercise groups were higher than those before exercise, and HSP72 expression levels in subgroup 1 were obviously higher than those in subgroup 2 and control group. The contents of ALT, urea, Na+, Cl-, Ca2+ and K+ in subgroups 1 and 2 were lower than those in control group, but CK level was higher than in control group (P<0.05). The contents of Na+ and Clin subgroup 1 were relatively lower than those in subgroup 2 (P<0.05). It was concluded that while receiving exercise at high temperature, adult males' HSP72 levels in PBLs could be increased and the biochemical indexes changed. Attention should be paid to health supervision to avoid obvious body injuries at high temperature.展开更多
The experiment was set up for examining the physiological and biological indexes quickly and exactly, for obtaining information of tobacco-field fertilizing and tobacco growing. The ASD Field spec FR 2500 was used to ...The experiment was set up for examining the physiological and biological indexes quickly and exactly, for obtaining information of tobacco-field fertilizing and tobacco growing. The ASD Field spec FR 2500 was used to measure spectra reflectance of flue-cured tobacco and the relationship between hyperspectral parameters and biochemical contents (total nitrogen, chlorophyll, carotenoid), and physiological indexes (fresh weight, dry weight, moisture content) of flue-cured tobacco leaves was studied by correlation and stepwise regression statistic methods at different nitrogen and potassium levels. The results indicated that the spectra curves of different treatments had obvious rules and great diversities. There were high correlations between different types of spectra parameters and ten physiological and biochemical indexes of flue-cured tobacco leaves. Hyperspectral characteristic variables of ten physiological and biochemical indexes were found through stepwise regression, and SDr/SDb was the characteristic variable closest to seven biochemical contents. Simultaneously, the R2 and regression coefficient of equations reached 0.05 significant level and the equations had good estimating effects through the examination of other samples. Accordingly, this study suggested that the ten physiological and biochemical indexes could be estimated quickly by the estimating models, at the same time nitrogen-potassium fertilization and growth condition of flue-cured tobacco could be inspected.展开更多
基金Supported by Animal Medicine Specialized Group Program of Beijing Characteristic High Level Backbone Specialized Group Project(PXM2020-157102-000060)General Project of Science and Technology Plan of Beijing Municipal Education Commission(KM201512448004)+1 种基金Scientific and Technological Capacity Enhancement Project of"Vegetable Basket"New Production and Business Entity of Beijing Municipal Agricultural Commission(20150203-5)Doctoral Fund of Beijing Vocational College of Agriculture(XY-BS-15-01).
文摘[Objective]The paper was to study the effects of fermentation bed padding on intestinal flora,serum biochemical indexes and immune function of growing-finishing pigs.[Method]A total of 90 growing-finishing pigs(Duroc × Landrace × Yorkshire)with the body weight of(30.5±0.8)kg were randomly divided into five groups,three replicates each group,six pigs each replicate.The padding of five groups were:50%rice husk+50%sawdust(control group),100%spent mushroom substrate of Pleurotus eryngii(100%spent mushroom substrate group),15%rice husk+15%sawdust+70%spent mushroom substrate(70%spent mushroom substrate group),25%rice husk+25%sawdust+50%spent mushroom substrate(50%spent mushroom substrate group),35%rice husk+35%sawdust+30%spent mushroom substrate(30%spent mushroom substrate group).The pre-trial lasted 7 d,and the formal trial lasted 90 d.[Result]Compared with the control group,the number of Escherichia col in 100%,70%and 50%spent mushroom substrate groups decreased by 35.4%,24.0%and 20.8%,respectively(P<0.05);the number of salmonella successively decreased by 29.4%,19.3%and 15.2%,respectively(P<0.05);the number of lactobacillus successively increased by 21.7%,15.9%and 12.2%,respectively(P<0.05);and the number of bifidobacteria successively increased by 20.2%,16.7%and 11.8%,respectively(P<0.05).Compared with the control group,the total serum protein content in 100%,70%,50%and 30%spent mushroom substrate groups increased by 30.6%,33.0%,28.0%and 18.4%,respectively(P<0.05);and the serum urea nitrogen content decreased by 32.4%,34.5%,33.9%and 22.0%,respectively(P<0.05).There was no significant difference in serum albumin,aspartate aminotransferase(AST)and alanine aminotransferase(ALT)between treatment groups and control group.The IgA,IgG,IgM and IL-6 levels in serum of each treatment group were significantly different from those of the control group(P<0.05).There was no significant difference in IgA,IgG,IgM and IL-6 levels among 100%,70%and 50%spent mushroom substrate groups.[Conclusion]It improves the balance of intestinal microecological flora of growing-finishing pigs and enhances the immune performance of pigs by using spent mushroom substrate of P.eryngii as the fermentation bed padding.15%rice husk+15%sawdust+70%spent mushroom substrate is the optimal proportion of spent mushroom substrate for growing-finishing pigs.
基金Supported by the Ford University Research Projectthe "Peak of Six Major Human Resources Plan" of Jiangsu Province
文摘To evaluate the acute lung toxicity and the effect on serum biochemical indexes of inhaled TiO2 nanotubes , healthy and adult Kunming mice are exposed to aerosols of TiO2nanotubes in a sealed chamber , the concentration of which is 250mg / m 3 while another group of mice are exposed to room air only served as control.The blood , alveolar lavaged fluid and lungs of the mice are collected and examined after exposed for 7 , 14and 28d , respectively.The serum analysis shows that glucose ( Glu ) values with 7dexposure , alanine aminotransferase ( ALT ) and aspartate aminotransferase ( AST ) activities with 28dinhalation , and total bile acid ( TBA ) values with 7 , 28d as well as the creatine kinase ( CK ) levels with 28dexposure are significantly different from those of controls ( P< 0.05 ) .The pathological findings exhibit that more TiO 2 nanotubes are found in the interstitium of pulmonary alveoli with the experimental time prolonged.The results suggest that TiO 2 nanotubes do produce adverse responses to the lung and serum biochemical indexes of mice.Moreover , the responses become severer with the exposed-time prolonged.
基金supported by Science and Technology Achievement Transformation Projects of Henan Province (0722011120002)
文摘[Objective] To investigate the effects of traditional Chinese medicine compound preparation on serum biochemical indexes and egg nutritional quality of laying hens. [Method] A total of 1 200 chickens at the age of 44 weeks were selected and randomly divided into five groups. After administration with different dosages of astragalus polysaccharides and berberine for 9 weeks,serum biochemical indexes were determined and nutrient contents of egg were detected. [Result] The contents of total protein,globulin,high-density lipoprotein cholesterol(HDL-C) and alkaline phosphatase(ALP) in all treatment groups were significantly(P < 0.05 or 0.01) higher than those in the control group. The cholesterol contents of all treatment groups were significantly(P < 0.05 or 0.01) lower than those of the control group. [Conclusion] The optimal concentrations of astragalus polysaccharides and berberine in basic diets are 200 and 30 mg/kg,respectively.
基金Supported by Gansu Academy of Agricultural Sciences Youth Fund Project(2014GAAS33)Agricultural Science and Technology Innovation Project of Gansu Academy of Agricultural Sciences(2013GAAS04)
文摘[Objective]This experiment was conducted to study the effects of dietary betaine on performance,egg quality and serum biochemical parameters of laying hens under heat stress condition. [Method]A total of 600 22-week-old commercial Roman laying hens were randomly divided into five groups,with eight replicates in each group and 15 hens in each replicate. Group I was taken as the positive control group,and laying hens in group I were fed with basal diets in normal thermal environment. Temperature humidity index of group I was between 64. 9 and 68. 9. Group II was taken as the negative control group,and laying hens in group II were fed with basal diets under heat stress condition. Temperature humidity index of group II was higher than 72. Laying hens in groups III-V were fed with basal diets supplemented with 200,400 and 600 mg/kg betaine under heat stress condition respectively,and temperature humidity indexes in groups III-V were all higher than 72. The experiment lasted for 14 weeks. [Results]Results showed that there were no significant differences in average daily feed intake,feed egg ratio and broken egg rate among different test groups( P > 0. 05). Hen-housed laying rate,hen-housed egg yield,serum total protein( TP) content and serum alkaline phosphatase( AKP) activity in group II were significantly lower than those in group I( P < 0. 05). However,activities of glutamic-oxalacetic transaminase( GOT),creatine kinase( CK) and glutamic-pyruvic transaminase( GPT) in group II were significantly higher than those in group I( P < 0. 05). Hen-housed laying rate,hen-housed egg yield and serum TP content in group IV were significantly higher than those in group II( P < 0. 05). Hen-housed egg yield,serum TP content and serum albumin( ALB) content in group V were significantly higher than those in group II( P < 0. 05). Activities of serum CK and GPT in groups IV and V were significantly lower than those in group II( P < 0. 05),serum triglyceride( TG) content in group V was significantly lower than that in group II( P < 0. 05).[Conclusion]Metabolism and physiological function of laying hens could be affected by heat stress,and which caused a decline in performance. Adding of dietary betaine in diets could improve hen-housed egg production and hen-housed laying rate,which could also improve the health of laying hens under heat stress condition. The appropriate dietary adding content of betaine was 400 mg/kg.
基金Supported by Science and Technology Development Program of Nanning City(20155181)Key Technology Research and Development Program of Guangxi Province(14121003-3-3)
文摘[Objective] This trial was to investigate the effect of dietary Picria felterrae Lour extracts on serum biochemical indexes and immune function of Guangxi Partridge chickens. [Method] Totally 500 individuals of 1-day-old Guangxi Partridge chickens were randomly divided into five groups, five replicates each group and 20 chicks each replicate. Groups A, B and C were treatment groups supplemented with 0.70%, 0.35% and0.175% P. felterrae extracts in the basal diet, respectively; group D was a medical control group supplemented with 0.01% colistin sulfate premix in the basal diet; and group E was a control group with basal diet. Serum samples were collected from 10 chickens in each group at 21, 35 and 49 days of age, to analyze the biochemical and immune indexes. [Result] It had no significant impact on serum TP and GLU contents of chickens by adding different levels of P. felterrae extracts in the basal diet(P >0.05). However, at 21 and 35 days of age, the TBILI content of chickens in groups A, B and C were extremely lower or significantly lower than that in group E( P<0.01; P<0.05). At 35 days of age, the serum AKP activities in groups A, B and C were extremely higher than that in group E(P<0.01), and the serum GOT activities at 21 and 35 days of age in group C were significantly higher than that in group E(P<0.05). At 49 days of age, the serum Ig G content in groups A, B and C and the IL-2 content in group B and group C were significantly lower or extremely lower than that in group E( P<0.05; P<0.01); the T-AOC content in group B was significantly higher than that in group E(P<0.05); the GSH content in groups A, B and C were significantly higher than that in group E( P<0.05). Adding different levels of P. felterrae extracts in the diet significantly decreased the TBILI content, reduced the Ig G, IL-2 and IL-6 contents, and improved the AKP and GOT activities and the T-AOC and GSH contents of Guangxi Partridge chicken. [Conclusion] Adding 0.35% P. felterrae extracts in the basal diet received the best effort.
文摘This study investigated the effects of protein sources for milk replacers on growth performance and serum biochemical indexes of suckling calves. Fifty Chinese Holstein bull calves with similar BW and age were randomly allocated to 5 groups(1 control and 4 treatments) of 10 calves in each group. Five types of milk replacers were designed to have the same level of energy and protein. The protein source for milk replacers of the control group was full milk protein(MP). The protein source of milk replacers of the 4 treatment groups was composed of MP and one vegetable protein(VP)(30 and 70% of total protein). The4 types of VP were soybean protein concentrate(SP), hydrolyzed wheat protein(WP), peanut protein concentrate(PP), and rice protein isolate(RP). Results of the experiment showed: 1) there was no significant difference on average daily gain(ADG) and feed:gain ratio(F:G) among the MP, SP and RP groups(P > 0.05), whereas the ADG and F:G of the WP and PP groups were significantly lower compared with the MP group(P < 0.05); 2) there was not a significant difference in withers height, body length and heart girth among treatment groups compared with the MP group(P > 0.05). Thereby the 4 VP milk replacers had no adverse effects on body size of calves; 3) all groups showed no significant difference in the serum contents of urea nitrogen, total protein, albumin, globulin, β-hydroxybutyrate, growth hormone, insulin-like growth factor-1, and the ratio of albumin to globulin(A:G)(P> 0.05). In conclusion, SP or RP(accounts for 70% of the total protein) as calf milk replacers could substitute MP, whereas wheat gluten and PP had a significant adverse effect on growth performance in this experiment.
基金Special Project for Technical Development of Hainan Scientific Research Institutions(SQ2017JSKF0008)China Agricultural Industry Research System(CARS-43-42)
文摘[Objective] The study was carried out to investigate the effects of dietary crude fiber on growth performance and serum biochemical in-dex of Dingan geese aged from 35 to 70 days.[Method] A total of 120 Dingan geese on day 28 with the same genetic background and similar initial weight(1 384.83.73±0.76 g) were randomly assigned into 3 groups, with 4 replicates per group and 10 geese per replicate. Geese were fed the diets containing 4.29%, 5.29% and 6.29 % crude fiber, respectively. The experiment lasted 35 d.[Result] Final weight and daily gain of 5.29 %CF group were significantly higher than those of 6.29 % CF group(P<0.05);Feed intake was markedly higher than those of 4.29% and 6.29 % CF groups(P<0.05);Feed gain ratio was significantly lower than that of 6.29 % CF group(P<0.05). Compared with 4.29% and 6.29 % CF groups, serum albumin concentrations of 5.29 % CF group increased significantly(P<0.05), while serum total protein and globin contents were higher(P>0.05), but serum uric acid level was lower(P>0.05).[Conclusion] 5.29 % crude fiber could lead to higher growth performance of Dingan geese.
基金Supported by Major Science and Technology Program of Hainan Province(ZDKJ2016017-01,ZDKJ2016017-03)
文摘[Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with the same genetic backgrounds and similar initial weight of(10.05±0.049) kg were randomly assigned into two treatments, and supplied with Juncao + concentrate(control group), pelleted total mixed ration(experimental group), respectively. The trial lasted 50 d.[Result] The final weight, daily gain and feed intake of experimental group were significantly higher than those of control group(P<0.05), but the feed/gain ratio of experimental group was markedly lower than that of control group(P<0.05). Compared with the control group, the gross profit of experimental group significantly increased( P <0.05).[Conclusion] Pelleted total mixed ration led to better production performance of growing Hainan black goat.
基金supported by National Natural Science Foundation of China(31772612)the Beijing Municipal Natural Science Foundation(6202019).
文摘Background:Sows are frequently subjected to various stresses during late gestation and lactation,which trigger inflammatory response and metabolic disorders.Dietary fiber can influence animal health by modulating gut microbiota and their by-products,with the effects depending upon the source of the dietary fiber.This study aimed to evaluate the impacts of different fiber sources on body condition,serum biochemical parameters,inflammatory responses and fecal microbiota in sows from late gestation to lactation.Methods:Forty-five multiparous sows(Yorkshire×Landrace;3–6 parity)were assigned to 1 of 3 dietary treatments from d 85 of gestation to the end of lactation(d 21 post-farrowing):a control diet(CON,a corn-soybean meal diet),a sugar beet pulp diet(SBP,20%SBP during gestation and 10%SBP during lactation),and a wheat bran diet(WB,30%WB during gestation and 15%WB during lactation).Results:Compared with CON,supplementation of SBP decreased(P<0.05)lactation BW loss,reduced(P<0.05)serum concentration of total cholesterol,non-esterified fatty acids,interleukin-6 and tumor necrosis factor-α,and increased(P<0.05)fecal water content on d 110 of gestation and d 21 of lactation,while supplementation of WB reduced(P<0.05)serum concentration of total cholesterol on d 110 of gestation,increased(P<0.05)fecal water content and decreased(P<0.05)serum interleukin-6 concentration on d 110 of gestation and d 21 of lactation.In addition,sows fed SBP had lower(P<0.01)abundance of Clostridium_sensu_stricto_1 and Terrisporobacter than those fed CON,but had greater(P<0.05)abundance of Christensenellaceae_R-7_group and Ruminococcaceae_UCG-002 than those fed the other two diets on d 110 of gestation.On d 21 of lactation,supplementation of SBP decreased(P<0.05)the abundance of Firmicutes and Lactobacillus,but enriched(P<0.05)the abundance of Christensenellaceae_R-7_group,Prevotellaceae_NK3B31_group,Ruminococcaceae_UCG-002,Prevotellaceae_UCG_001 and unclassified_f__Lachnospiraceae compared with WB.Compared with CON,sows fed SBP had greater(P<0.05)fecal concentrations of acetate,butyrate and total SCFAs during gestation and lactation,while sows fed WB only had greater(P<0.05)fecal concentration of butyrate during lactation.Conclusions:Supplementation of dietary fiber during late gestation and lactation could improve sow metabolism and gut health,and SBP was more effective than WB.
文摘This experiment was conducted to evaluate the effect of onion (Allium cepa L.) as an antibiotic growth promoter substitute on growth performance, immune responses and serum biochemistry in broilers. A total of 192 one-d-old as hatched broiler chicks (Ross 308) were weighed and randomly allocated to four treatment groups, each with 4 replicate pens of 12 chicks. The dietary treatments consisted of the basal diet (control), antibiotic (15 mg Virginiamycin/kg), and control +10 or 30 g fresh onion bulb/kg diet. Body weights of broilers were determined at d 1, 21 and 42, feed intake was determined at the same periods, and feed conversion ratio was calculated accordingly. At 14th and 21st days blood samples were taken for measuring antibody titers against NDV and at 42nd day for biochemical analysis. At d 42, two birds per replicate were slaughtered for determination of lymphoid organ weights. Dietary supplementation of30 g/kg onion increased final body weight of broilers at 42nd d of age compared to the other treatments (P < 0.05). Birds fed30 gonion/kg in the diet had the highest feed intake than other treatments at different growth periods (P < 0.05). Dietary treatments failed to induce any significant effect on antibody titers against NDV, although the weight of lymphoid organs was significantly (P Broilers receiving30 g/kg onion had a significantly higher HDL and lower triglyceride concentrations compared to control groups (P . Feeding30 g/kg onion resulted in a marked reduction in the concentration of the glucose compared to control groups (P . The results suggested that dietary inclusion of30 g/kg onion can be applied as alternatives to in-feed antibiotics for broiler diets.
基金Supported by Major Science and Technology Program of Hainan Province(ZDKJ2016017-01,ZDKJ2016017-03)
文摘[Objective] The paper to investigate the effects of pelleted total mixed ration of different particle size on production performance and serum biochemical index of fattening Hainan black goats.[Method] Thirty-six fattening Hainan black goats with the same genetic backgrounds and similar initial weight of(19.02±0.22) kg were randomly divided into three treatments. Goats were fed with Juncao+concentrate(control group), pel-leted total mixed ration I(particle size 6 mm, experimental group I) and II(particle size 5 mm, experimental group II), respectively. The trial lasted 45 d.[Result](1) The final weight and daily gain of experimental group II were significantly higher than those of control group( P<0.05), and the daily gain of experimental group II was also higher than that of experimental group I(P<0.05);the feed intake of experimental group II was markedly higher than that of control group(P<0.05), but was significantly lower than that of experimental group I(P<0.05). The feed/gain ratio of experimental group II was significantly lower than those of control group and experimental group I(P<0.05).(2) The feed gain cost of experimental group II was markedly lower than that of control group(P<0.05), but the gross profit was significantly higher than those of control group and experimental group I(P<0.05).(3) Compared with the control group, the serum total protein content of experimental group II increased markedly( P<0.05) while the urea nitrogen level decreased(P>0.05).[Conclusion] The pelleted total mixed ration with the particle size of 5 mm obtained better fattening ef-fect, and obviously improved the serum total protein level.
基金Supported by Special Fund for Technology Development of Hainan Scientific Research Institute(KYYS-2019-04)Natural Science Foundation of Hainan Province(318MS109)+1 种基金Special Fund for Construction of China Agricultural Industry Research System(CARS-43-42)Special Funds for Local Science and Technology Development Guided by the Central Committee(ZY2019HN01)
文摘[Objective]The paper was to investigate the effects of dietary crude fiber level on production performance,organ index and serum biochemical parameters of Dingan geese aged from 1 to 28 days.[Method]A total of 144 1-day-old Dingan geese with the same genetic background and similar initial weight(88.75±0.21g)were randomly assigned into 3 groups with 4 replicates per group and 12 geese per replicate,and fed with the diets containing 2.56%,3.52% and 4.48% crude fiber,respectively.The trial lasted 28 d.[Result]1)The final weight and daily gain of 3.52% CF group were significantly higher than those of 2.56% CF group(P<0.05);the feed/gain ratio was markedly lower than that of 2.56% CF group(P<0.05).2)The slaughter rate,semi-eviscerated rate and leg muscle rate of 3.52% CF group were higher than those of 2.56% and 4.48% CF groups(P>0.05).3)As the dietary crude fiber levels rose,the abdominal fat rate showed a downward trend(P>0.05).4)The liver index of 3.52% CF group was significantly higher than that of 2.56% CF group(P<0.05).4)Compared with 2.5%and 4.48% CF groups,the serum albumin concentrations of 3.52% CF group markedly increased(P<0.05),the serum total protein and globin contents were higher(P>0.05),but the serum uric acid level was lower(P>0.05).[Conclusion]When the crude fiber level was 3.52%,the production performances of Dingan geese aged from 1 to 28 days were better,and the liver index and serum albumin level could be obviously improved.
文摘In order to provide reference for using Siganus guttatus to control overgrowth of Enteromorpha prolifra,the growth,serum biochemical and antioxidant enzyme indices of juvenile S.guttatus respectively fed with E.prolifra and artificial feed were studied.One hundred and eighty individuals were cultivated for 90 days in six 2.5 m × 1.5 m × 1.5 m cages (30 ind.per cage) which mesh size were 0.5 mm.The experimental animals were divided into two dietary groups (three cages for each group) that were fed with E.prolifra and artificial feed respectively.During the culture period,temperatures ranged from 23.0 to 26.5℃,p H was between 7.8 and 8.2,dissolved oxygen was more than 5.0 mg/L.The weight gain rate,specific growth rate,relative growth rate,hepato-somatic index of juvenile S.guttatus fed with E.prolifra were significantly lowe than that fed with artificial feed (P<0.05).The total protein (TP),urea nitrogen (UN)and alkaline phosphatase (AKP) of juvenile S.guttatus fed with E.Prolifra were significantly higher than that fed with artificial feed (P<0.05),while glutamic oxalacetic transaminase (AST/GOT) and glutamic-pyruvic transaminase (ALT/GPT) of juvenile S.guttatus fed with E.prolifra were significantly lower than that fed with artificial feed (P<0.05).SOD,GPX and anti-superoxide anion radical activity in liver,kidney,gill and muscle of juvenile S.guttatus fed with E.prolifra were higher than that fed with artificial feed,and these antioxidant enzyme activities in kidney of juvenile S.guttatus fed with E.prolifra was significantly higher than that fed with artificial feed (P<0.05),while CAT and hydroxyl radical-inhibiting activity in liver of juvenile S.guttatus fed with artificial feed were significantly higher than that fed with E.prolifra (P<0.05).Juvenile S.guttatus fed with E.prolifra showed poor growth performance but better antioxidant defense system.S.guttatus is easily raised and has stronger ability of digestion and absorption of E.Prolifra.The technique that uses S.guttatus to control E.prolifra deserves deeply study.
基金Technical Development Project of Hainan Provincial Scientific Research Institutes(KYYS-2018-05)Special Funds for Central Government Guiding the Development of Local Science and Technology(ZY2019HN01).
文摘[Objective]The paper was to investigate the effects of dietary concentrate to forage ratios on production performance and serum bio-chemical indicators of post-fattening Hainan Yellow Cattle.[Method]Sixteen Hainan Yellow Cattles with the same genetic backgrounds and similar initial weight of(242.22 ±0.71 kg) were randomly divided into four treatments.Cattles were fed with diets of different concentrate to forage ratios(20:80, group Ⅰ;30:70, Group Ⅱ;40:60, group Ⅲ;50:50, group Ⅳ).The trial lasted 62 d.[Result]The daily gain in experimental group Ⅳ was significantly higher than that in experimental group Ⅱ(P<0.05), but the feed gain ratio in experimental group Ⅳ was lower than those in other ex-perimental groups(P>0.05).The feed intake in experimental group Ⅳ was also markedly higher than those in experimental groups Ⅰ and Ⅱ(P<0.05).Compared with the experimental group Ⅱ, the gross profit of experimental group Ⅳ was significantly promoted(P<0.05).The alanine aminotransferase and aspartate aminotransferase activities in experimental group Ⅱ were markedly higher than those in experimental groups Ⅰ, Ⅲ and Ⅳ.[Conclusion]When the dietary concentrate to forage ratios was 50∶50, the production performance of post-fattening Hainan Yellow Cattle was better.
文摘Purpose: We evaluated biochemical analysis results with the aim of discovering serum levels that have possible effects on the development of retinopathy of prematurity (ROP). Methods: A retrospective study was conducted between January 2017 and January 2018 on a total of 110 infants with 35 or less gestational weeks. The sample included 78 infants who had been diagnosed with different stages of ROP and 32 infants without ROP. Results from routine serum biochemical analyses, performed at birth and at one month after birth, were evaluated. The Independent Sample t-test and Mann-Whitney U test were performed to compare the data. Results: The infants with ROP were born at a mean of 28.0 (±2.1) weeks of gestation, weighing a mean of 1066 (±314) g, and the mean duration of stay in the incubator was 38.2 (±19) days. The infants without ROP were born at a mean of 29.6 (±2.6) weeks of gestation, weighing a mean of 1265 (±372) g. 59 infants (53.6%) were females, and 51 (46.4%) were males. Between infants with and without ROP, differences in the following levels were not statistically significant: glucose, blood urine nitrogen, creatinine, alanine aminotransferase, aspartate aminotransferase, albumin, total protein, direct bilirubin, indirect bilirubin, uric acid, and phosphorus. Serum magnesium (Mg) levels at birth in infants with ROP were significantly higher than infants without ROP (p = 0.014). Conclusions: Serum Mg levels at birth were found to be higher in infants with ROP than in those without ROP. The levels may be dependent on the mothers’ treatment of Mg for different medical reasons.
文摘The objective of this study was to investigate the effects of phytase supplementation on growth performance, slaughter performance, growth of internal organs and small intestine, and serum biochemical indices of broilers. A total of 360 1-day-old Ross 308 broilers were randomly divided into 4 groups with 3 replicates and 30 broilers per replicate (15 male, 15 female). The treatments were fed with basal diet supplemented with 0%, 0.01%, 0.02% and 0.03% phytase. The results showed that: 1) Phytase supplementation increased the body weight gain and the body weight of Ross 308 broilers (P serum calcium (Ca) consistency was 0.02%. According to the above analysis, the feasible supplementation of phytase (enzyme activity for 5000 u/g) in broiler dietary was 0.02%.
基金Supported by Key Research and Development Program of Hebei Province(18226603D)Modern Agricultural Science and Technology Innovation Project of Hebei Province(4-04-03)
文摘In order to study the effects of replacing different proportions of silage maize with silage sweet sorghum treated by different fermentation methods on the production performance and blood biochemical indexes of dairy cows,25 Chinese Holstein lactating cows were randomly divided into 5 groups,5 in each group.The control check (CK) was fed the basal diet;for the experimental group 1 and the experimental group 2,50% of the silage maize in the basal diet was replaced with the additive silage sweet sorghum and the conventional silage sweet sorghum,respectively;and as to the experimental group 3 and the experimental group 4,all the silage maize in the basal diet was replaced with additive silage sweet sorghum and conventional silage sweet sorghum,respectively.The preliminary trial period was 7 d,and the trial period was 35 d.The results showed that the experimental group 1 and the experimental group 3 had the dry matter intake significantly higher than that of the experimental group 2,the experimental group 4 and the CK ( P <0.05).The daily milk yields of the experimental group 1 and the experimental group 2 were significantly lower than that of the CK ( P <0.05),and the experimental group 3 and the experimental group 4 were significantly lower than the experimental group 1 and the experimental group 2 ( P <0.05).The milk protein percentage of the experimental group 2 was extremely significantly higher than that of the experimental group 4 ( P <0.01).The experimental group 1,the experimental group 2 and the experimental group 3 showed the blood glucose levels extremely significantly higher than that in the CK ( P <0.01).The blood urea nitrogen contents in the experimental group 2 and the experimental group 4 were significantly higher than those in the experimental group 1 and the CK ( P <0.01).There were no significant differences in other blood biochemical indexes between various groups ( P >0.05).It is feasible to use silage sweet sorghum to feed dairy cows,but the proportion should not be too large,and attention should be paid to the energy and nitrogen balance of the diet.
基金supported by the Major Special Project of Guangdong Province, China (2009A080303009)the Special Fund for Public Welfare Industry of China (Agriculture, 201003011)+2 种基金the National 948 Project of China (2011-G35)the National Major Science Research Program of China (2009CB941601)the Joint Funds of the National Natural Science Foundation of China (U0731004)
文摘To investigate the effects of dietary supplementation with hydrolyzed wheat gluten (HWG) on growth performance, cell immunity and serum biochemical indices of weaned piglets, 160 crossed (Large White×andrace) and weaned piglets were randomly divided into 4 treatments with 4 replicates of 10 piglets each. The piglets in each treatment were fed an experimental diet containing either 0 g kg-1 HWG (control group), 30 g kg-1 HWG (3% HWG group), 50 g kg-1 HWG (5% HWG group), or 2.5 g kg-1 glycyl-L-glutamine (0.25% Gly-Gln group). The results showed that the diarrhea rates in 3% HWG and 5% HWG groups were significantly lower than in control group from d 1 to 14 (P<0.05), while the average daily gain (ADG) in each of two groups was increased (P>0.05). When compared with control group, dietary supplementation with 3% HWG increased the ratio of CD4+:CD8+ and the ratio of serum albumin and globulin concentrations (A:G) on d 14 and 28, as well as the proliferation of T- and B-lymphocytes (P>0.05) on d 28. In addition, on d 14 and 28, the A:G ratio in 5% HWG group was significantly higher than in control group (P<0.05), while the ratio of CD4+:CD8+ increased slightly (P>0.05). Interestingly, 0.25% Gly-Gln group had higher proportion of CD3+ (P>0.05) and CD4+ (P<0.05) on d 14 than control group, but growth performances of 0.25% Gly-Gln group were negatively affected at all experiment stages. These results suggested that HWG might improve the growth performance of piglets by strengthening cell immunity and decreasing the occurrence of diarrhea during the prophase after weaning.
文摘The influence of exercise at high temperature on adult males' routine blood indexes and biochemical indexes and the expression of HSP72 in peripheral blood lymphocytes (PBLs) was studied in order to provide theoretical ground for health supervision of adults receiving exercise at high temperature. 180 adult males were selected and divided into exercise group and control group, in which the exercise group was subdivided into subgroup 1 and subgroup 2 receiving exercise at high temperature in the afternoon and in the morning, respectively. Peripheral venous blood was phle- botomized before and after the exercise to examine routine blood indexes and blood biochemical indexes. The expression levels of HSP72 in PBLs were detected by flow cytometry. The results showed that the routine blood indexes and biochemical indexes in each group were within the range of normal values of male adults. There was no significant difference between each exercise group and control group in indexes before exercise. After exercise, the expression levels of HSP72 in PBLs in exercise groups were higher than those before exercise, and HSP72 expression levels in subgroup 1 were obviously higher than those in subgroup 2 and control group. The contents of ALT, urea, Na+, Cl-, Ca2+ and K+ in subgroups 1 and 2 were lower than those in control group, but CK level was higher than in control group (P<0.05). The contents of Na+ and Clin subgroup 1 were relatively lower than those in subgroup 2 (P<0.05). It was concluded that while receiving exercise at high temperature, adult males' HSP72 levels in PBLs could be increased and the biochemical indexes changed. Attention should be paid to health supervision to avoid obvious body injuries at high temperature.
文摘The experiment was set up for examining the physiological and biological indexes quickly and exactly, for obtaining information of tobacco-field fertilizing and tobacco growing. The ASD Field spec FR 2500 was used to measure spectra reflectance of flue-cured tobacco and the relationship between hyperspectral parameters and biochemical contents (total nitrogen, chlorophyll, carotenoid), and physiological indexes (fresh weight, dry weight, moisture content) of flue-cured tobacco leaves was studied by correlation and stepwise regression statistic methods at different nitrogen and potassium levels. The results indicated that the spectra curves of different treatments had obvious rules and great diversities. There were high correlations between different types of spectra parameters and ten physiological and biochemical indexes of flue-cured tobacco leaves. Hyperspectral characteristic variables of ten physiological and biochemical indexes were found through stepwise regression, and SDr/SDb was the characteristic variable closest to seven biochemical contents. Simultaneously, the R2 and regression coefficient of equations reached 0.05 significant level and the equations had good estimating effects through the examination of other samples. Accordingly, this study suggested that the ten physiological and biochemical indexes could be estimated quickly by the estimating models, at the same time nitrogen-potassium fertilization and growth condition of flue-cured tobacco could be inspected.
