The present work aimed to investigate the microscopic and ultramicroscopic structure of the liver to assess the level of hepatic impairment during pregnancy toxemia. Seven pregnant small-tailed Han sheep of negative u...The present work aimed to investigate the microscopic and ultramicroscopic structure of the liver to assess the level of hepatic impairment during pregnancy toxemia. Seven pregnant small-tailed Han sheep of negative urine ketone bodies were assessed in this study. Toxemia was induced by limiting food and movement late in pregnancy. Three sheep developed obvious clinical symptoms with motor weakness, depression, anorexia, locomotion disturbances, blindness and languishment. We harvested their liver tissues as pathological material, and used routine histological and electronic microscopy methods to observe the histopathological changes in small-tailed Han sheep induced by pregnancy toxemia. Autopsy of the livers of the sheep revealed deep yellow coloration, intumescence and hemorrhage on the surface. Microstructural features indicated fatty degeneration, which is a main characteristic of fatty liver. Hepatocellular ultrastructural changes were observed under an electronic microscope. The characteristic findings were nucleolus concentration, vacuolation of mitochondria and excessive glycogen granules in the cytoplasm. Via this experimental protocol, pregnancy toxemia of sheep was successfully induced, providing a pathological model for the study of this disease. After the experimental induction of pregnancy toxemia, the clinical symptoms of pathogenic sheep and pathological changes to their livers exhibited obvious characteristics of pregnancy toxemia.展开更多
本研究以湖羊肝脏为材料对肝受体类似物-1(Liver receptor homolog-1,Lrh-1;NR5A2)基因序列进行RT-PCR和RACE测定,并用DNAman、Tmpred、Signal P 3.0、Expasy等生物信息学分析软件和在线工具,对Lrh-1cDNA序列及其蛋白的理化特性、跨膜...本研究以湖羊肝脏为材料对肝受体类似物-1(Liver receptor homolog-1,Lrh-1;NR5A2)基因序列进行RT-PCR和RACE测定,并用DNAman、Tmpred、Signal P 3.0、Expasy等生物信息学分析软件和在线工具,对Lrh-1cDNA序列及其蛋白的理化特性、跨膜结构、信号肽和二级结构进行生物信息学分析,同时采用实时荧光定量PCR方法检测湖羊Lrh-1基因的组织表达谱。结果表明,湖羊Lrh-1基因cDNA序列全长1 488bp,与牛的核酸序列相似度最高,为98%,编码区共编码495个氨基酸,氨基酸序列与牛、人、马、猴、犬、小鼠、褐鼠的氨基酸同源性分别为98%、97%、96%、97%、97%、86%和86%;Lrh-1mRNA在湖羊脑、消化及生殖系统组织中均有表达且具有组织特异性,其中在下丘脑组织中的表达量相对最高。展开更多
基金Supported by Natural Science Foundation of Ningxia(BA002-2004)
文摘The present work aimed to investigate the microscopic and ultramicroscopic structure of the liver to assess the level of hepatic impairment during pregnancy toxemia. Seven pregnant small-tailed Han sheep of negative urine ketone bodies were assessed in this study. Toxemia was induced by limiting food and movement late in pregnancy. Three sheep developed obvious clinical symptoms with motor weakness, depression, anorexia, locomotion disturbances, blindness and languishment. We harvested their liver tissues as pathological material, and used routine histological and electronic microscopy methods to observe the histopathological changes in small-tailed Han sheep induced by pregnancy toxemia. Autopsy of the livers of the sheep revealed deep yellow coloration, intumescence and hemorrhage on the surface. Microstructural features indicated fatty degeneration, which is a main characteristic of fatty liver. Hepatocellular ultrastructural changes were observed under an electronic microscope. The characteristic findings were nucleolus concentration, vacuolation of mitochondria and excessive glycogen granules in the cytoplasm. Via this experimental protocol, pregnancy toxemia of sheep was successfully induced, providing a pathological model for the study of this disease. After the experimental induction of pregnancy toxemia, the clinical symptoms of pathogenic sheep and pathological changes to their livers exhibited obvious characteristics of pregnancy toxemia.
