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Investigation of the Acceptability of Cultured Meat in University Students
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作者 Merve Kumru Hulya Demir 《Food and Nutrition Sciences》 CAS 2024年第2期151-169,共19页
Background: Over the past 20 years, cultured meat has drawn a lot of public attention as a potential solution to issues with animal husbandry, including inadequate use of natural sources, improper animal welfare pract... Background: Over the past 20 years, cultured meat has drawn a lot of public attention as a potential solution to issues with animal husbandry, including inadequate use of natural sources, improper animal welfare practices, and possible risks to public health and safety. The novel method of producing meat through culture reduces the need for animals to produce muscle fiber, thereby obviating the necessity for animal slaughter. Apart from its ethical advantages, cultured meat presents a possible way to fulfill the expanding need for food among growing populations. The purpose of this research was to find out whether Turkish students would be willing to pay for and accept cultured meat. Technique: Method: 371 university students who willingly consented to fill out a questionnaire and provide demographic data make up the research sample. Questions from previous studies on the acceptability of cultured meat were compiled to create the survey. The research’s data collection took place in March and April of 2022. The research was completed in June 2022 after the data had been processed and analyzed. Results: The results showed that the majority of participants were female and had omnivorous eating habits. Based on the results of the Bonferroni correction test, students with a higher intention to purchase and consume cultured meat were those who received economics and business education. Students with two years of university education had a higher overall survey score than those with four years of education (p < 0.05). Furthermore, it is discovered that there is a negative correlation between the participants’ ages and their Factor 2 (using cultured meat as an alternative to industrial meat) and Factor 3 (consuming and purchasing it) section points (r = -109, p = 0.036) (r = -0.121, p = 0.019). In conclusion, university students generally have a negative outlook on health-related issues, such as eating cultured meat as an alternative. 展开更多
关键词 cultured Meat University Students
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Preparation of lactic acid bacteria compound starter cultures based on pasting properties and its improvement of glutinous rice flour and dough
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作者 Dengyu Wang Linlin Liu +4 位作者 Bing Wang Wenjian Xie Yanguo Shi Na Zhang Hongchen Fan 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第4期2090-2101,共12页
The effects of 5 lactic acid bacteria(LAB)fermentation on the pasting properties of glutinous rice flour were compared,and suitable fermentation strains were selected based on the changes of viscosity,setback value,an... The effects of 5 lactic acid bacteria(LAB)fermentation on the pasting properties of glutinous rice flour were compared,and suitable fermentation strains were selected based on the changes of viscosity,setback value,and breakdown value to prepare LAB compound starter cultures.The results revealed that Latilactobacillus sakei HSD004 and Lacticaseibacillus rhamnosus HSD005 had apparent advantages in increasing the viscosity and reducing the setback and breakdown values of glutinous rice flour.In particular,the compound starter created using the two abovementioned LAB in the ratio of 3:1 had better performance than that using a single LAB in improving the pasting properties and increasing the water and oil absorption capacity of glutinous rice flour.Moreover,the gelatinization enthalpy of the fermented samples increased significantly.For frozen glutinous rice dough stored for 28 days,the viscoelasticity of frozen dough prepared by compound starter was better than that of control dough,and the freezable water content was lower than that of control dough.These results indicate that compound LAB fermentation is a promising technology in the glutinous rice-based food processing industry,which has significance for its application. 展开更多
关键词 Glutinous rice flour Glutinous rice dough Lactic acid bacteria compound starter cultures Pasting properties VISCOELASTICITY
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High-quality Development of the Pearl River Night Tour in the Context of Deep Integration of Culture and Tourism
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作者 Juan JIANG Lianrong PANG 《Meteorological and Environmental Research》 2024年第1期38-45,共8页
Urban night tourism is a new form of urban tourism development and an important driving force for the prosperity of urban night economy.As the most prestigious business card among many night tour projects in Guangzhou... Urban night tourism is a new form of urban tourism development and an important driving force for the prosperity of urban night economy.As the most prestigious business card among many night tour projects in Guangzhou,the Pearl River water night tour has contributed to the development of Guangzhou s night economy,which cannot be underestimated.However,in the process of rapid development,there are also problems such as diversified operators,insufficient integration of intangible cultural elements,excessive commercial attributes,issues of tourism facilities and service facilities.Under the new stage of integrated development of culture and tourism,how to use mutual promotion of culture and tourism in the Pearl River night tour to seek innovative paths for high-quality development in the future is the main problem expected to be solved in this paper.Therefore,based on the perspective of culture and tourism integration,this paper analyzes the general situation and development status of the Pearl River night tour,and proposes five development strategies:the overall planning of culture and tourism integration,the deep integration of intangible cultural elements,the balance of commercial attributes and tourism attributes,the high integration of culture,business,tourism and education,and the creation of tourism atmosphere,in order to provide inspiration for the healthy,orderly and high-quality development of the Pearl River night tour. 展开更多
关键词 Integration of culture and tourism The Pearl River night tour High-quality development Intangible cultural elements
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Consumer Culture in The Great Gatsby
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作者 ZHU Yuan-chen 《Journal of Literature and Art Studies》 2024年第2期124-127,共4页
F.Scott Fitzgerald’s The Great Gatsby successfully portrays the“Jazz Age”.In the early 20th century,the United States transitioned from a production-based society to a consumer-based society,leading to an era of un... F.Scott Fitzgerald’s The Great Gatsby successfully portrays the“Jazz Age”.In the early 20th century,the United States transitioned from a production-based society to a consumer-based society,leading to an era of unparalleled prosperity.However,in the context of consumer culture,individuals often transition from being masters of their possessions to becoming enslaved by them.As a result,their behaviours and values become increasingly associated with extravagance and consumption.This paper uses Baudrillard’s theory of consumer society and textual analysis to explore the impact of consumer culture on human beings through the symbolization of men and objectification of women in The Great Gatsby.The meanings embedded in the novel are revealed from a different perspective. 展开更多
关键词 consumer culture SYMBOLS OBJECTIFICATION The Great Gatsby
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Coupling coordination analysis of Mazu culture,socio-economy and ecological environment of Meizhou Island
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作者 FU Wei-jia WU Ying-qi +2 位作者 LUO Zi-xuan YAO Jun-bing FU Li-jun 《Ecological Economy》 2024年第1期85-100,共16页
Taking the pilgrimage,tourism and cultural island of Meizhou Island as an example,the evaluation index system of the coupling and coordinated development of“Mazu culture,socio-economy,eco-environment”(MSE)compound s... Taking the pilgrimage,tourism and cultural island of Meizhou Island as an example,the evaluation index system of the coupling and coordinated development of“Mazu culture,socio-economy,eco-environment”(MSE)compound system was constructed.The index weights were determined by AHP-entropy method,and the coupling degree,coordinated degree,comprehensive evaluation index and grey correlation degree of MSE system of Mazu Island from 2012 to 2022 were measure.The results showed that:(1)the comprehensive evaluation indexes of the three subsystems was on the rise in general,but the evaluation index of the ecological subsystems increased relatively slowly.(2)The coupling degree was only in the running-in stage in 2012,and the other years were in the coordinated coupling stage.(3)The coupling coordination degree increased from 0.35 in 2012 to 0.82 in 2022,the coupling coordination level was changed from mild imbalance to good coordination.(4)Through the comparison of grey correlation degree,the 24 indexes in the evaluation index system had great influence on the coupling coordination degree of MSE system.The coupling coordination degree was closely related to the development of socio-economy and the spread of Mazu culture.With the rapid development of tourism brought about by the spread of Mazu culture,the pressure on the ecological environment will be increasing.Compared with the rapid growth of tourism and economy,it is equally important to strengthen environmental protection and pay attention to the quality of ecological environment development. 展开更多
关键词 Meizhou Island Mazu culture socio-economy ecological environment comprehensive evaluation index coupling coordination degree
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The Cult of Mazu in Hainan,China and Among Vietnamese Hainanese Diaspora:A Comparative Study of Mazu Culture
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作者 ZHU Si YOU Qi-Dongmeng 《Cultural and Religious Studies》 2024年第1期42-47,共6页
The cult of Mazu is a common folk belief in China and Vietnam.Originating from the Song and Yuan Dynasties,particularly during the Ming and Qing Dynasties,the cult of Mazu spread to Vietnam through maritime trade and ... The cult of Mazu is a common folk belief in China and Vietnam.Originating from the Song and Yuan Dynasties,particularly during the Ming and Qing Dynasties,the cult of Mazu spread to Vietnam through maritime trade and migration from coastal regions of China.After centuries of integration and development,it evolved into its own cultural system of Mazu worship,becoming an important component of Vietnamese folk culture.Hainan,China,as a crucial supply station on the Maritime Silk Road,has close connections with the transmission of Mazu culture to Vietnam.This paper adopts field investigations and literature research methods to explore the Mazu worship and culture in Hainan,China,and the Qiongfu Assembly Halls in Vietnam. 展开更多
关键词 Mazu worship and culture HAINAN Vietnamese Hainanese diaspora comparison
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Value and Measures of Integrating Local Red Culture into the Ideological and Political Course of Environmental Disciplines under the OBE Concept
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作者 Xiong YAN Wenzhao LI +5 位作者 Shenwen CAI Bailian XIONG Liumei CHEN Junwei CHENG Qinghe WANG Leilei FAN 《Asian Agricultural Research》 2024年第2期40-42,共3页
Based on output-oriented education,the OBE(Outcome-Based Education)concept integrates local red culture into the ideological and political course of environmental disciplines,and is an important part of training appli... Based on output-oriented education,the OBE(Outcome-Based Education)concept integrates local red culture into the ideological and political course of environmental disciplines,and is an important part of training applied talents of environmental disciplines in the new era.This educational model makes an innovation on the traditional educational and teaching concepts and centers on students.This paper analyzes the value of integrating local red culture into the ideological and political course under the OBE concept,and puts forward an effective implementation path. 展开更多
关键词 OBE(Outcome-Based Education)concept Local red culture Environmental disciplines Ieological and political course
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A Study on the Integration of Chinese Culture in High School English Teaching
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作者 Hui Zhong Dandan Song 《Journal of Contemporary Educational Research》 2024年第3期55-59,共5页
The inseparable relationship between language and culture indicates that culture teaching is indispensable in English teaching.The ultimate goal of culture teaching in English teaching is to cultivate learners’cross-... The inseparable relationship between language and culture indicates that culture teaching is indispensable in English teaching.The ultimate goal of culture teaching in English teaching is to cultivate learners’cross-cultural communication skills.Cross-cultural communication is a two-way process,which requires not only knowledge of English culture but also the use of English as a medium for disseminating Chinese culture to the outside world.However,in actual English teaching,the teaching of Chinese culture is a mere formality,and the teaching effect is not obvious.Therefore,it is necessary to conduct a survey and research on Chinese culture teaching in high school English classrooms to understand its current situation,existing problems,and their causes,so as to provide suggestions for Chinese culture teaching in high school. 展开更多
关键词 Chinese culture High school English teaching INTEGRATION
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An Introduction to the Dissemination of Chinese Culture in English Teaching in Colleges and Universities
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作者 Hui Fang 《Journal of Contemporary Educational Research》 2024年第1期116-120,共5页
Language and culture are closely related,and understanding the cultural knowledge embedded in the process of language learning is an important goal of English teaching in colleges and universities.At present,the disse... Language and culture are closely related,and understanding the cultural knowledge embedded in the process of language learning is an important goal of English teaching in colleges and universities.At present,the dissemination of Chinese culture in English teaching in colleges and universities is lacking,which affects the improvement of students’intercultural communication skills.In order to effectively solve such problems,we need to take effective measures to disseminate Chinese culture by combining teaching content and students’characteristics.This paper summarizes the problems of Chinese culture dissemination in English teaching in colleges and universities,and explores effective countermeasures and teaching considerations,hoping to provide teachers with valuable guidelines. 展开更多
关键词 Colleges and universities English teaching Chinese culture
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Exploration of the Inheritance and Innovation Carrier of National Culture in the Teaching Model of Art Design Major in Higher Vocational Colleges:Integrating Lijiang Baisha Murals into Higher Vocational Art Design Curriculum System
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作者 Tingting Wang 《Journal of Contemporary Educational Research》 2024年第1期165-172,共8页
This study aims to further promote the inheritance and innovative development of intangible cultural heritage in Yunnan Province,promote the protection,inheritance,integration,and innovation of ethnic culture in Lijia... This study aims to further promote the inheritance and innovative development of intangible cultural heritage in Yunnan Province,promote the protection,inheritance,integration,and innovation of ethnic culture in Lijiang,strengthen the protection and inheritance of Baisha murals in Lijiang,and change the teaching of art design majors in vocational colleges in Yunnan.Given the lack of traditional Chinese culture and local ethnic culture,this article focuses on the teaching of art design majors in Yunnan vocational colleges.It explores the construction model of the cultural inheritance and innovation carrier of Lijiang Baisha murals to meet the spiritual and cultural needs of the local people,and efforts will be made to promote the high-quality development of the Baisha ethnic area in Lijiang. 展开更多
关键词 Baisha murals National culture Inheritance and innovation Art design major
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A Study on the Pedagogical Application of Chinese New Year Culture in International Chinese Language Education
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作者 Xiaoxuan Ma Yong Hu 《Journal of Contemporary Educational Research》 2024年第3期27-31,共5页
Culture teaching is an inseparable part of International Chinese Language Education,and it has great significance in international Chinese language teaching.Students can enhance their interest in Chinese language lear... Culture teaching is an inseparable part of International Chinese Language Education,and it has great significance in international Chinese language teaching.Students can enhance their interest in Chinese language learning and improve their learning effect through understanding the excellent traditional Chinese culture,at the same time,culture teaching can help to disseminate the Chinese culture and promote the development of a multicultural world.Taking the teaching of Chinese New Year culture as an example,we analyze the current teaching materials involving Chinese New Year culture with high popularity and acceptance,put forward effective teaching methods matching the feasibility of teaching Chinese New Year culture,and then present the specific teaching design of Chinese New Year culture,in order to provide references for the teaching of culture in International Chinese Language Education. 展开更多
关键词 International Chinese Language Education Chinese New Year culture Pedagogical application
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Integrating Salt Culture into Rural Revitalization: Planning and Design of a Thematic Creative Agricultural Garden in Jintan, Jiangsu, China
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作者 Chengwang Yang 《Journal of Architectural Research and Development》 2024年第2期1-7,共7页
This research establishes a strategic framework for developing a salt culture-themed agricultural garden in Jintan,Jiangsu to boost rural vibrancy and cultural tourism while honoring local salt heritage.It employs the... This research establishes a strategic framework for developing a salt culture-themed agricultural garden in Jintan,Jiangsu to boost rural vibrancy and cultural tourism while honoring local salt heritage.It employs the Analytic Hierarchy Process(AHP)for a data-driven and structured approach to evaluate the sustainability and cultural significance of agricultural initiatives.This framework ensures a balanced blend of agriculture,culture,and tourism to foster a sustainable and culturally rich experience.The study highlights the value of structured methodologies in planning culturally impactful agricultural projects. 展开更多
关键词 Landscape garden Rural revitalization Theme creative farm Salt culture Planning and design
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英语Culture time板块教学初探 被引量:1
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作者 顾洁 《教育研究与评论(小学教育教学)》 2023年第4期58-60,共3页
译林版小学英语教材从五年级上册开始增设Culture time板块,目的是让学生在学习文化知识的过程中实现文化理解。指向文化理解的Culture time板块教学,可以通过补充相关知识,帮助学生理解文化的多样性;通过引导探究体验,帮助学生理解文... 译林版小学英语教材从五年级上册开始增设Culture time板块,目的是让学生在学习文化知识的过程中实现文化理解。指向文化理解的Culture time板块教学,可以通过补充相关知识,帮助学生理解文化的多样性;通过引导探究体验,帮助学生理解文化的深刻性;通过指引对比异同,帮助学生理解文化的差异性。 展开更多
关键词 小学英语 culture time板块 文化理解
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Few-Shot Object Detection Based on the Transformer and High-Resolution Network 被引量:1
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作者 Dengyong Zhang Huaijian Pu +2 位作者 Feng Li Xiangling Ding Victor S.Sheng 《Computers, Materials & Continua》 SCIE EI 2023年第2期3439-3454,共16页
Now object detection based on deep learning tries different strategies.It uses fewer data training networks to achieve the effect of large dataset training.However,the existing methods usually do not achieve the balan... Now object detection based on deep learning tries different strategies.It uses fewer data training networks to achieve the effect of large dataset training.However,the existing methods usually do not achieve the balance between network parameters and training data.It makes the information provided by a small amount of picture data insufficient to optimize model parameters,resulting in unsatisfactory detection results.To improve the accuracy of few shot object detection,this paper proposes a network based on the transformer and high-resolution feature extraction(THR).High-resolution feature extractionmaintains the resolution representation of the image.Channels and spatial attention are used to make the network focus on features that are more useful to the object.In addition,the recently popular transformer is used to fuse the features of the existing object.This compensates for the previous network failure by making full use of existing object features.Experiments on the Pascal VOC and MS-COCO datasets prove that the THR network has achieved better results than previous mainstream few shot object detection. 展开更多
关键词 Object detection few shot object detection TRANSFORMER HIGH-RESOLUTION
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Distribution and Drug Resistance Analysis of 2287 Strains of Pathogenic Bacteria in Children’s Blood Culture 被引量:1
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作者 Tiefu Fang Qiang Wang +3 位作者 Wanqi Li Yanhuan Mao Peiqing Li Guangming Liu 《Advances in Microbiology》 CAS 2023年第1期24-31,共8页
Background: Bloodstream infection is a serious infectious disease. In recent years, the drug resistance of pathogenic bacteria to commonly used anti-infective drugs has been widely concerned, which also makes the trea... Background: Bloodstream infection is a serious infectious disease. In recent years, the drug resistance of pathogenic bacteria to commonly used anti-infective drugs has been widely concerned, which also makes the treatment of bloodstream infection face severe challenges. Objective: To explore the distribution characteristics of blood culture-positive pathogens and the resistance to antibacterial drugs, so as to provide clinicians with accurate laboratory evidence, so as to guide clinicians to rationally apply antibiotics, improve clinical treatment effects, and reduce the emergence of drug-resistant strains. Methods: From January 2019 to June 2022, 2287 positive blood culture specimens of patients in Guangzhou Women and Children’s Medical Center were retrospectively analyzed, and the proportion of different pathogenic bacteria, the distribution of pathogenic bacteria in different departments, and the multi-drug resistance of different pathogenic bacteria were counted. Results: Among the 2287 blood culture positive samples, 1560 strains (68.20%) of gram-positive bacteria and 727 strains (31.80%) of gram-negative bacteria were strained. The top three departments in the distribution of pathogenic bacteria were pediatric intensive care unit (600 strains), pediatric internal medicine (514 strains), and pediatric emergency comprehensive ward (400 strains). The pathogens with high detection rates were: Staphylococcus epidermidis (24.09%), Staphylococcus humans (23.74%), Escherichia coli (13.21%) and Klebsiella pneumoniae (8.71%). The pathogens with high multi-drug resistance rates were: Streptococcus pneumoniae (93%), Staphylococcus epidermidis (83.76%), Enterobacter cloacae (75.61%) and Staphylococcus humans (62.43%). Conclusion: In our hospital, gram-positive bacteria were the main pathogenic bacteria in the blood culture of children patients. The children’s intensive care unit was the department with the largest distribution of pathogenic bacteria, and the multiple drug resistance rate of Streptococcus pneumoniae was the highest. 展开更多
关键词 CHILDREN Blood culture Pathogen Drug Resistance
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Range estimation of few-shot underwater sound source in shallow water based on transfer learning and residual CNN 被引量:1
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作者 YAO Qihai WANG Yong YANG Yixin 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2023年第4期839-850,共12页
Taking the real part and the imaginary part of complex sound pressure of the sound field as features,a transfer learning model is constructed.Based on the pre-training of a large amount of underwater acoustic data in ... Taking the real part and the imaginary part of complex sound pressure of the sound field as features,a transfer learning model is constructed.Based on the pre-training of a large amount of underwater acoustic data in the preselected sea area using the convolutional neural network(CNN),the few-shot underwater acoustic data in the test sea area are retrained to study the underwater sound source ranging problem.The S5 voyage data of SWellEX-96 experiment is used to verify the proposed method,realize the range estimation for the shallow source in the experiment,and compare the range estimation performance of the underwater target sound source of four methods:matched field processing(MFP),generalized regression neural network(GRNN),traditional CNN,and transfer learning.Experimental data processing results show that the transfer learning model based on residual CNN can effectively realize range estimation in few-shot scenes,and the estimation performance is remarkably better than that of other methods. 展开更多
关键词 transfer learning residual convolutional neural network(CNN) few shot vertical array range estimation
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“中国文化”英语课程思政教学设计与实践——以Culinary Culture为例
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作者 罗文 《湖北开放职业学院学报》 2023年第13期186-188,共3页
课程思政是当前高等教育改革的重要方向之一。本文分析了“中国文化”英语课程思政建设的意义及现状,以教学单元Culinary Culture为例,从教学设计理念、课程思政元素、教学目标和方法、具体实施过程和教学效果等方面探究课程思政建设路... 课程思政是当前高等教育改革的重要方向之一。本文分析了“中国文化”英语课程思政建设的意义及现状,以教学单元Culinary Culture为例,从教学设计理念、课程思政元素、教学目标和方法、具体实施过程和教学效果等方面探究课程思政建设路径,落实语言教学和立德育人的双重任务。 展开更多
关键词 中国文化 英语课程 思政教学设计
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The role of forest culture in green construction and management of forest parks:merging knowledge-attitude-practice and theory of planned behavior 被引量:1
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作者 Yao Xu Zhiguang Zhang 《Journal of Forestry Research》 SCIE CAS CSCD 2023年第3期841-852,共12页
As a cultural concept refl ecting the relationship between humans and forests,forest culture plays an active role in sustainable forest management.Forest parks provide a wide range of ecosystem services essential for ... As a cultural concept refl ecting the relationship between humans and forests,forest culture plays an active role in sustainable forest management.Forest parks provide a wide range of ecosystem services essential for the sustainable development of society,and the relationships between forest culture,green construction and management of forest parks have practical signifi cance.This study aimed to understand the interaction and process of forest culture infl uencing green construction and management in forest parks with the models Knowledge-Attitude-Practice(KAP)and Theory of Planned Behavior(TPB)by proposing a theoretical model.Four hypotheses were tested using data collected from 193 forest park employees in Heilongjiang Province,China.Our results show that forest culture had a signifi cant infl uence on green construction and forest management.In addition,subjective norm and perceived behavioral control directly impacted behavior in green construction and management of the forest park,whereas attitude did not have an impact.Subjective norm had a direct eff ect on attitude.Results between constructs show that forest culture had an indirect eff ect on planning and construction,and on ecological and economic management.Consequently,it supported three of four hypotheses within the proposed model in determining the infl uence of forest culture on green construction and management. 展开更多
关键词 Forest parks Forest culture Green construction and management Knowledge-attitudepractice Theory of planned behavior
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Phytochemical Contents and Antioxidant Properties of Ganoderma lucidum Polysaccharides in Relation to Growth Stage and Culture Substrate 被引量:1
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作者 Guoying LU Aizhen HE +4 位作者 Jinrong CHEN Zhouwei XU Chaolian HE Jianfei CHEN Zuofa ZHANG 《Medicinal Plant》 CAS 2023年第3期5-8,14,共5页
[Objectives]To determine physicochemical characteristics and antioxidant activity of Ganoderma lucidum polysaccharides(GLP)in different growth stages and culture media.[Methods]Five polysaccharides(GLP1,GLP2,GLP3,GLPW... [Objectives]To determine physicochemical characteristics and antioxidant activity of Ganoderma lucidum polysaccharides(GLP)in different growth stages and culture media.[Methods]Five polysaccharides(GLP1,GLP2,GLP3,GLPW and GLPB)were extracted and purified from the fruiting body of G.lucidum at three growth stages and culture substrates(wood culture and bag culture).The chemical components and antioxidant activity of the five polysaccharides were determined.[Results]GLP1 contained the highest content of neutral sugar(87.65%)and GLP3 had the highest uronic acid content.All the samples mainly comprised glucose,galactose,mannose,xylose and arabose with different ratios.Moreover,their antioxidant activities were investigated on the basis of DPPH radical,ABTS radical,SOD-like activity,and antihaemolytic activity.Results indicated in all three growth stages GLP2 had the greatest antioxidant properties.In addition,the antioxidant activity of GLPW was significantly higher than that of GLPB.[Conclusions]Overall,by comparison,G.lucidum in growth stage two may have potential health benefits,and wood culture may be a superior choice of artificial cultivation due to their abundance of active polysaccharides. 展开更多
关键词 Ganoderma lucidum POLYSACCHARIDES Antioxidant activity Growth stage culture substrate
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Long-term in-vitro culture and subculture of the hemocytes of swimming crab Portunus trituberculatus
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作者 Liwen GUO Yaqi ZHAO Huarong GUO 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2023年第5期1918-1939,共22页
Crab cell line,especially continuous crab cell line,can provide us a useful tool for studies on the virology,immunology,and molecular biology of crabs.However,no continuous crab cell line has been available due to the... Crab cell line,especially continuous crab cell line,can provide us a useful tool for studies on the virology,immunology,and molecular biology of crabs.However,no continuous crab cell line has been available due to the lacking of suitable medium and the occurrence of mitosis-arrest.In this study,long-term in vitro culture conditions for both two-(2D)and three-dimensions(3D)were successfully developed for the circulating hemocytes of swimming crab Portunus trituberculatus,designated as PTH cells.In 2D culture,a novel crab basic medium in osmolarity of 990–1100 mOsm/kg was optimized for the first time,which is different from Leibovitz's L-15 medium in mainly the components of amino acids,containing double strengths of the contents of free amino acid mixture in the crab serum.Then an optimal crab growth medium was developed by supplementing 5%fetal bovine serum,50-g/L yeast extract powder,20-μg/L basic fibroblast growth factor and epidermal growth factor into the optimal crab basic medium,and found that it could support a long-term survival of PTH cells in a healthy monolayer up to 347 days and partially break through the mitosis-arrest of crab cells evidenced by the obvious increase of proliferating potential detected in the 10-d primarily cultured PTH cells.These 2D cultured PTH cells could be successfully sub-cultured for 11 times by physical flushing method and well cryopreserved in liquid nitrogen.In 3D culture,using the same crab growth medium,the PTH cell aggregates could be easily formed and healthily maintained on the surface of solidified Matrigel or in the ultra-low-attachment plate with a survival rate of 50%–60%on Day 103.This work largely improved the primary culture and subculture of crab cells and will facilitate the establishment of continuous crab cell line. 展开更多
关键词 CRAB Portunus trituberculatus HEMOCYTE long-term cell culture SUBculture 3D culture
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