Background:Colon adenocarcinoma(COAD)is a gastrointestinal malignancy with a high mortality rate.Studies have confirmed the role of immunogenic cell death(ICD)in different cancer types.However,there is a lack of resea...Background:Colon adenocarcinoma(COAD)is a gastrointestinal malignancy with a high mortality rate.Studies have confirmed the role of immunogenic cell death(ICD)in different cancer types.However,there is a lack of research on ICD-related genes(ICD-RGs)in COAD.This study aimed to examine the impact of ICD-RGs on COAD and their interaction with the immune microenvironment.Methods:Using data from The Cancer Genome Atlas and Gene Expression Omnibus databases,we identified 107 ICD-RGs in COAD.Using a one-way Cox regression analysis,we examined the relationship between these ICD-RGs and overall survival in COAD.Results:Following the regression analyses,we identified 14 overall survival-related genes.Furthermore,we examined the predictive impact of the ICD-RGs using the least absolute shrinkage and selection operator regression analysis and developed a nine-genes prognostic model.The Cancer Genome Atlas and Gene Expression Omnibus datasets were used for training and validation.Kaplan-Meier analysis was used to confirm that the high-risk group had a lower survival rate than the low-risk group.Finally,following a multifactorial analysis,we created a prognostic nomogram that integrated clinical data and risk scores.Conclusions:The nine-genes model exhibits robust stability and can provide valuable insights for guiding the development of tumor immunotherapy strategies and personalized drug selection for patients with COAD.展开更多
Triple-negative breast cancer(TNBC)poses a significant challenge due to the lack of reliable prognostic gene signatures and an understanding of its immune behavior.Methods:We analyzed clinical information and mRNA exp...Triple-negative breast cancer(TNBC)poses a significant challenge due to the lack of reliable prognostic gene signatures and an understanding of its immune behavior.Methods:We analyzed clinical information and mRNA expression data from 162 TNBC patients in TCGA-BRCA and 320 patients in METABRIC-BRCA.Utilizing weighted gene coexpression network analysis,we pinpointed 34 TNBC immune genes linked to survival.The least absolute shrinkage and selection operator Cox regression method identified key TNBC immune candidates for prognosis prediction.We calculated chemotherapy sensitivity scores using the“pRRophetic”package in R software and assessed immunotherapy response using the Tumor Immune Dysfunction and Exclusion algorithm.Results:In this study,34 survival-related TNBC immune gene expression profiles were identified.A least absolute shrinkage and selection operator-Cox regression model was used and 15 candidates were prioritized,with a concomitant establishment of a robust risk immune classifier.The high-risk TNBC immune groups showed increased sensitivity to therapeutic agents like RO-3306,Tamoxifen,Sunitinib,JNK Inhibitor VIII,XMD11-85h,BX-912,and Tivozanib.An analysis of the Search Tool for Interaction of Chemicals database revealed the associations between the high-risk group and signaling pathways,such as those involving Rap1,Ras,and PI3K-Akt.The low-risk group showed a higher immunotherapy response rate,as observed through the tumor immune dysfunction and exclusion analysis in the TCGA-TNBC and METABRIC-TNBC cohorts.Conclusion:This study provides insights into the immune complexities of TNBC,paving the way for novel diagnostic approaches and precision treatment methods that exploit its immunological intricacies,thus offering hope for improved management and outcomes of this challenging disease.展开更多
BACKGROUND Single-cell sequencing technology provides the capability to analyze changes in specific cell types during the progression of disease.However,previous single-cell sequencing studies on gastric cancer(GC)hav...BACKGROUND Single-cell sequencing technology provides the capability to analyze changes in specific cell types during the progression of disease.However,previous single-cell sequencing studies on gastric cancer(GC)have largely focused on immune cells and stromal cells,and further elucidation is required regarding the alterations that occur in gastric epithelial cells during the development of GC.AIM To create a GC prediction model based on single-cell and bulk RNA sequencing(bulk RNA-seq)data.METHODS In this study,we conducted a comprehensive analysis by integrating three singlecell RNA sequencing(scRNA-seq)datasets and ten bulk RNA-seq datasets.Our analysis mainly focused on determining cell proportions and identifying differentially expressed genes(DEGs).Specifically,we performed differential expression analysis among epithelial cells in GC tissues and normal gastric tissues(NAGs)and utilized both single-cell and bulk RNA-seq data to establish a prediction model for GC.We further validated the accuracy of the GC prediction model in bulk RNA-seq data.We also used Kaplan–Meier plots to verify the correlation between genes in the prediction model and the prognosis of GC.RESULTS By analyzing scRNA-seq data from a total of 70707 cells from GC tissue,NAG,and chronic gastric tissue,10 cell types were identified,and DEGs in GC and normal epithelial cells were screened.After determining the DEGs in GC and normal gastric samples identified by bulk RNA-seq data,a GC predictive classifier was constructed using the Least absolute shrinkage and selection operator(LASSO)and random forest methods.The LASSO classifier showed good performance in both validation and model verification using The Cancer Genome Atlas and Genotype-Tissue Expression(GTEx)datasets[area under the curve(AUC)_min=0.988,AUC_1se=0.994],and the random forest model also achieved good results with the validation set(AUC=0.92).Genes TIMP1,PLOD3,CKS2,TYMP,TNFRSF10B,CPNE1,GDF15,BCAP31,and CLDN7 were identified to have high importance values in multiple GC predictive models,and KM-PLOTTER analysis showed their relevance to GC prognosis,suggesting their potential for use in GC diagnosis and treatment.CONCLUSION A predictive classifier was established based on the analysis of RNA-seq data,and the genes in it are expected to serve as auxiliary markers in the clinical diagnosis of GC.展开更多
This study is intended to explore the chemical differences of Acori Tatarinowii Rhizoma （ATR） samples collected from two habitats, Sichuan and Anhui provinces, China. Gas chromatography-mass spectrometry （GC-MS） w...This study is intended to explore the chemical differences of Acori Tatarinowii Rhizoma （ATR） samples collected from two habitats, Sichuan and Anhui provinces, China. Gas chromatography-mass spectrometry （GC-MS） was applied to establishing the quantitative chemical fingerprints of ATRs. A total of 104 volatile compounds were identified and quantified with the information of mass spectra and retention index （RI）. Furthermore, least absolute shrinkage and selection operator （LASSO）, a sparse regularization method, combined with subsampling was employed to improve the classification ability of partial least squares-discriminant analysis （PLS-DA）. After variable selection by LASSO, three chemical markers,β-elemene, α-selinene and α-asarone, were identified for the discrimination of ATRs from two habitats, and the total classification correct rate was increased from 82.76% to 96.55%. The proposed LASSO-PLS-DA method can serve as an efficient strategy for screening marked chemical components and geo-herbalism research of traditional Chinese medicines.展开更多
<strong>Objective</strong>: This paper aims to explore clinical status and related influence factors of pressure injury (PI) in the elderly inpatients with kidney disease, so as to provide reference for th...<strong>Objective</strong>: This paper aims to explore clinical status and related influence factors of pressure injury (PI) in the elderly inpatients with kidney disease, so as to provide reference for the prevention and treatment of PI in the elderly inpatients with kidney disease. <strong>Methods</strong>: Retrospective collection method is adopted to collect 158 clinical cases of the elderly inpatients with kidney disease aged ≥ 60 in the Nephrology Department, the First Affiliated Hospital of Jinan University from January 2017 to December 2019, and then least absolute shrinkage and selection Operator (LASSO) regression analysis is used to analyze 17 possible influence factors;finally Logistic regression model is established to analyze and screen influence factors of risk. <strong>Results</strong>: 1) Among 158 elderly inpatients with medium and high risk of PI, the incidence of PI is 20.25%;the most common stage of injury is stage I (42.5%);sacrococcygeal (60%) is the high-risk site of pressure injury. 2) LASSO regression analysis shows that history of present respiratory infection/respiratory failure (<em>β </em>= 1.2714. <em>P</em> < 0.05) and hospitalization time (<em>β</em> = 0.4177. <em>P </em>< 0.05) are independent factors influencing PI risk in the elderly inpatients with kidney disease. <strong>Concl</strong><strong>usio</strong><strong>n</strong>: The elderly patients with kidney disease and PI risk are the high incidence population of hospital acquired PI;for the elderly inpatients with kidney disease and having respiratory infection history or respiratory failure, prolonged hospitalization will significantly increase the risk of PI. Therefore, targeted preventive and control measures should be taken to reduce the incidence of PI.展开更多
BACKGROUND Gastric cancer(GC)is one of the most frequently diagnosed gastrointestinal cancers throughout the world.Novel prognostic biomarkers are required to predict the prognosis of GC.AIM To identify a multi-long n...BACKGROUND Gastric cancer(GC)is one of the most frequently diagnosed gastrointestinal cancers throughout the world.Novel prognostic biomarkers are required to predict the prognosis of GC.AIM To identify a multi-long noncoding RNA(lncRNA)prognostic model for GC.METHODS Transcriptome data and clinical data were downloaded from The Cancer Genome Atlas.COX and least absolute shrinkage and selection operator regression analyses were performed to screen for prognosis associated lncRNAs.Receiver operating characteristic curve and Kaplan-Meier survival analyses were applied to evaluate the effectiveness of the model.RESULTS The prediction model was established based on the expression of AC007991.4,AC079385.3,and AL109615.2 Based on the model,GC patients were divided into“high risk”and“low risk”groups to compare the differences in survival.The model was re-evaluated with the clinical data of our center.CONCLUSION The 3-lncRNA combination model is an independent prognostic factor for GC.展开更多
Background:Lung adenocarcinoma is one of the most common pathological types of lung malignant tumor with high morbidity and mortality.Long non-coding RNAs are gradually recognized to play crucial roles in tumor occurr...Background:Lung adenocarcinoma is one of the most common pathological types of lung malignant tumor with high morbidity and mortality.Long non-coding RNAs are gradually recognized to play crucial roles in tumor occurrence and development.Necroptosis is a newly established way for cell programmed death,undertaking essential roles in anti-tumor.Therefore,identifying necroptosis-related l ong non-coding RNAs and based on them to evaluate the signatures of l ung adenocarcinoma is essential for patients’survival prediction and therapy.Methods:We collected data from the public database and performed the least absolute shrinkage to construct a 13-lncRNAs prognostic model.Based on the Consensus Clustering,ESTIMATE,CIRERSORT,and weighted gene co-expression network analysis to identify the immune signatures.Results:This study identified a 13-lncRNAs prognostic model.The model’s prediction accuracy was evaluated by receiver operating characteristic and independent-prognosis analysis;besides,a Gene Expression Omnibus dataset was applied for external validation.Furthermore,we analyzed the immune features of subgroups in multiple dimensions.A consensus clustering analysis based on the 41 genes was implemented to separate lung adenocarcinoma patients into two subgroups.We compared the features of subgroups in multiple dimensions,including survival,immune microenvironment,immune cells infiltration and gene co-expression network analysis.Conclusion:W e established a prognosis necroptosis-related risk model to predict lung adenocarcinoma patients’prognosis and systematically understood the correlation between immune and necroptosis.This study can applicate in clinical to predict the prognosis of lung adenocarcinoma patients and provide new insight into lung adenocarcinoma immune therapy.展开更多
Background:Dilated cardiomyopathy(DCM)has a high mortality rate and is the most common indication for heart transplantation.Our study sought to develop a multiparametric nomogram to assess individualized all-cause mor...Background:Dilated cardiomyopathy(DCM)has a high mortality rate and is the most common indication for heart transplantation.Our study sought to develop a multiparametric nomogram to assess individualized all-cause mortality or heart transplantation(ACM/HTx)risk in DCM patients.Methods:The present study is a retrospective cohort study.The demographic,clinical,blood test,and cardiac magnetic resonance imaging(CMRI)data of DCM patients in the tertiary center(Fuwai Hospital)were collected.The primary endpoint was ACM/HTx.The least absolute shrinkage and selection operator(LASSO)Cox regression model was applied for variable selection.Multivariable Cox regression was used to develop a nomogram.The concordance index(C-index),area under the receiver operating characteristic curve(AUC),calibration curve,and decision curve analysis(DCA)were used to evaluate the performance of the nomogram.Results:A total of 218 patients were included in the present study.They were randomly divided into a training cohort and a validation cohort.The nomogram was established based on eight variables,including mid-wall late gadolinium enhancement,systolic blood pressure,diastolic blood pressure,left ventricular ejection fraction,left ventricular end-diastolic diameter,left ventricular end-diastolic volume index,free triiodothyronine,and N-terminal pro-B type natriuretic peptide.The AUCs regarding 1-year,3-year,and 5-year ACM/HTx events were 0.859,0.831,and 0.840 in the training cohort and 0.770,0.789,and 0.819 in the validation cohort,respectively.The calibration curve and DCA showed good accuracy and clinical utility of the nomogram.Conclusions:We established and validated a circulating biomarker-and CMRI-based nomogram that could provide a personalized prediction of ACM/HTx for DCM patients,which might help risk stratification and decision-making in clinical practice.展开更多
This paper proposes a robust vector tracking loop structure based on potential bias analysis. The influence of four kinds of biases on the existing two implementations of Vector Tracking Loops(VTLs) is illustrated by ...This paper proposes a robust vector tracking loop structure based on potential bias analysis. The influence of four kinds of biases on the existing two implementations of Vector Tracking Loops(VTLs) is illustrated by theoretical analysis and numerical simulations, and the following findings are obtained. Firstly, the initial user state bias leads to steady navigation solution bias in the relative VTL, while new measurements can eliminate it in the absolute VTL. Secondly, the initial code phase bias is transferred to the following navigation solutions in the relative VTL, while new measurements can eliminate it in the absolute VTL. Thirdly, the user state bias induced by erroneous navigation solution of VTLs can be eliminated by both of the two VTLs. Fourthly,the multipath/NLOS likely affects the two VTLs, and the induced tracking bias in the duration of the multipath/NLOS would decrease the performance of VTLs. Based on the above analysis,a robust VTL structure is proposed, where the absolute VTL is selected for its robustness to the two kinds of initialization biases;meanwhile, the instant bias detection and correction method is used to improve the performance of VTLs in the duration of the multipath/NLOS. Numerical simulations and experimental results verify the effectiveness of the proposed robust VTL structure.展开更多
To enhance the performance of the prediction intervals (PIs), a novel very short-term probabilistic prediction method for wind speed via nonlinear quantile regression (NQR) based on adaptive least absolute shrinkage a...To enhance the performance of the prediction intervals (PIs), a novel very short-term probabilistic prediction method for wind speed via nonlinear quantile regression (NQR) based on adaptive least absolute shrinkage and selection operator (ALASSO) and integrated criterion (IC) is proposed. The ALASSO method is studied for shrinkage of output weights and selection of variables. Furthermore, for the better performance of PIs, composite weighted linear programming (CWLP) is proposed to modify the conventional linear programming cost function of quantile regression (QR), by combining it with Bayesian information criterion (BIC) as an IC to optimize the coefficients of PIs. Then, the multiple fold cross model (MFCM) is utilized to improve the PIs performance. Multistep probabilistic prediction of 15-minute wind speed is performed based on the real wind farm data from the northeast of China. The effectiveness of the proposed approach is validated through the performances' comparisons with conventional methods.展开更多
Background:Utilizing population-based survey data in epidemiological research with a spatial perspective can integrate valuable context into the dynamics of HIV prevalence in West Africa.However,the situation in the M...Background:Utilizing population-based survey data in epidemiological research with a spatial perspective can integrate valuable context into the dynamics of HIV prevalence in West Africa.However,the situation in the Mano River Union(MRU)countries is largely unknown.This research aims to perform an ecological study to determine the HIV prevalence patterns in MRU.Methods:We analyzed Demographic and Health Survey(DHS)and AIDS Indicator Survey(AIS)data on HIV prevalence in MRU from 2005 to 2020.We examined the country-specifc,regional-specifc and sex-specifc ratios of respondents to profle the spatial–temporal heterogeneity of HIV prevalence and determine HIV hot spots.We employed Geodetector to measure the spatial stratifed heterogeneity(SSH)of HIV prevalence for adult women and men.We assessed the comprehensive correct knowledge(CCK)about HIV/AIDS and HIV testing uptake by employing the Least Absolute Shrinkage and Selection Operator(LASSO)regression to predict which combinations of CCKs can scale up the ratio of HIV testing uptake with sex-specifc needs.Results:In our analysis,we leveraged data for 158,408 respondents from 11 surveys in the MRU.From 2005–2015,Cote d’Ivoire was the hot spot for HIV prevalence with a Gi_Bin score of 3,Z-Score 8.0–10.1 and P<0.001.From 2016 to 2020,Guinea and Sierra Leone were hot spots for HIV prevalence with a Gi_Bin score of 2,Z-Score of 3.17 and P<0.01.The SSH confrmed the signifcant diferences in HIV prevalence at the national level strata,with a higher level for Cote d’Ivoire compared to other countries in both sexes with q-values of 0.61 and 0.40,respectively.Our LASSO model predicted diferent combinations of CCKs with sex-specifc needs to improve HIV testing uptake.Conclusions:The spatial distribution of HIV prevalence in the MRU is skewed and the CCK about HIV/AIDS and HIV testing uptake are far below the threshold target set by UNAIDS for ending the epidemic in the sub-region.Geodetector detected statistically signifcant SSH within and between countries in the MRU.Our LASSO model predicted that diferent emphases should be implemented when popularizing the CCK about HIV/AIDS for adult women and men.展开更多
文摘Background:Colon adenocarcinoma(COAD)is a gastrointestinal malignancy with a high mortality rate.Studies have confirmed the role of immunogenic cell death(ICD)in different cancer types.However,there is a lack of research on ICD-related genes(ICD-RGs)in COAD.This study aimed to examine the impact of ICD-RGs on COAD and their interaction with the immune microenvironment.Methods:Using data from The Cancer Genome Atlas and Gene Expression Omnibus databases,we identified 107 ICD-RGs in COAD.Using a one-way Cox regression analysis,we examined the relationship between these ICD-RGs and overall survival in COAD.Results:Following the regression analyses,we identified 14 overall survival-related genes.Furthermore,we examined the predictive impact of the ICD-RGs using the least absolute shrinkage and selection operator regression analysis and developed a nine-genes prognostic model.The Cancer Genome Atlas and Gene Expression Omnibus datasets were used for training and validation.Kaplan-Meier analysis was used to confirm that the high-risk group had a lower survival rate than the low-risk group.Finally,following a multifactorial analysis,we created a prognostic nomogram that integrated clinical data and risk scores.Conclusions:The nine-genes model exhibits robust stability and can provide valuable insights for guiding the development of tumor immunotherapy strategies and personalized drug selection for patients with COAD.
文摘Triple-negative breast cancer(TNBC)poses a significant challenge due to the lack of reliable prognostic gene signatures and an understanding of its immune behavior.Methods:We analyzed clinical information and mRNA expression data from 162 TNBC patients in TCGA-BRCA and 320 patients in METABRIC-BRCA.Utilizing weighted gene coexpression network analysis,we pinpointed 34 TNBC immune genes linked to survival.The least absolute shrinkage and selection operator Cox regression method identified key TNBC immune candidates for prognosis prediction.We calculated chemotherapy sensitivity scores using the“pRRophetic”package in R software and assessed immunotherapy response using the Tumor Immune Dysfunction and Exclusion algorithm.Results:In this study,34 survival-related TNBC immune gene expression profiles were identified.A least absolute shrinkage and selection operator-Cox regression model was used and 15 candidates were prioritized,with a concomitant establishment of a robust risk immune classifier.The high-risk TNBC immune groups showed increased sensitivity to therapeutic agents like RO-3306,Tamoxifen,Sunitinib,JNK Inhibitor VIII,XMD11-85h,BX-912,and Tivozanib.An analysis of the Search Tool for Interaction of Chemicals database revealed the associations between the high-risk group and signaling pathways,such as those involving Rap1,Ras,and PI3K-Akt.The low-risk group showed a higher immunotherapy response rate,as observed through the tumor immune dysfunction and exclusion analysis in the TCGA-TNBC and METABRIC-TNBC cohorts.Conclusion:This study provides insights into the immune complexities of TNBC,paving the way for novel diagnostic approaches and precision treatment methods that exploit its immunological intricacies,thus offering hope for improved management and outcomes of this challenging disease.
文摘BACKGROUND Single-cell sequencing technology provides the capability to analyze changes in specific cell types during the progression of disease.However,previous single-cell sequencing studies on gastric cancer(GC)have largely focused on immune cells and stromal cells,and further elucidation is required regarding the alterations that occur in gastric epithelial cells during the development of GC.AIM To create a GC prediction model based on single-cell and bulk RNA sequencing(bulk RNA-seq)data.METHODS In this study,we conducted a comprehensive analysis by integrating three singlecell RNA sequencing(scRNA-seq)datasets and ten bulk RNA-seq datasets.Our analysis mainly focused on determining cell proportions and identifying differentially expressed genes(DEGs).Specifically,we performed differential expression analysis among epithelial cells in GC tissues and normal gastric tissues(NAGs)and utilized both single-cell and bulk RNA-seq data to establish a prediction model for GC.We further validated the accuracy of the GC prediction model in bulk RNA-seq data.We also used Kaplan–Meier plots to verify the correlation between genes in the prediction model and the prognosis of GC.RESULTS By analyzing scRNA-seq data from a total of 70707 cells from GC tissue,NAG,and chronic gastric tissue,10 cell types were identified,and DEGs in GC and normal epithelial cells were screened.After determining the DEGs in GC and normal gastric samples identified by bulk RNA-seq data,a GC predictive classifier was constructed using the Least absolute shrinkage and selection operator(LASSO)and random forest methods.The LASSO classifier showed good performance in both validation and model verification using The Cancer Genome Atlas and Genotype-Tissue Expression(GTEx)datasets[area under the curve(AUC)_min=0.988,AUC_1se=0.994],and the random forest model also achieved good results with the validation set(AUC=0.92).Genes TIMP1,PLOD3,CKS2,TYMP,TNFRSF10B,CPNE1,GDF15,BCAP31,and CLDN7 were identified to have high importance values in multiple GC predictive models,and KM-PLOTTER analysis showed their relevance to GC prognosis,suggesting their potential for use in GC diagnosis and treatment.CONCLUSION A predictive classifier was established based on the analysis of RNA-seq data,and the genes in it are expected to serve as auxiliary markers in the clinical diagnosis of GC.
基金Project(21465016)supported by the National Natural Foundation of China
文摘This study is intended to explore the chemical differences of Acori Tatarinowii Rhizoma （ATR） samples collected from two habitats, Sichuan and Anhui provinces, China. Gas chromatography-mass spectrometry （GC-MS） was applied to establishing the quantitative chemical fingerprints of ATRs. A total of 104 volatile compounds were identified and quantified with the information of mass spectra and retention index （RI）. Furthermore, least absolute shrinkage and selection operator （LASSO）, a sparse regularization method, combined with subsampling was employed to improve the classification ability of partial least squares-discriminant analysis （PLS-DA）. After variable selection by LASSO, three chemical markers,β-elemene, α-selinene and α-asarone, were identified for the discrimination of ATRs from two habitats, and the total classification correct rate was increased from 82.76% to 96.55%. The proposed LASSO-PLS-DA method can serve as an efficient strategy for screening marked chemical components and geo-herbalism research of traditional Chinese medicines.
文摘<strong>Objective</strong>: This paper aims to explore clinical status and related influence factors of pressure injury (PI) in the elderly inpatients with kidney disease, so as to provide reference for the prevention and treatment of PI in the elderly inpatients with kidney disease. <strong>Methods</strong>: Retrospective collection method is adopted to collect 158 clinical cases of the elderly inpatients with kidney disease aged ≥ 60 in the Nephrology Department, the First Affiliated Hospital of Jinan University from January 2017 to December 2019, and then least absolute shrinkage and selection Operator (LASSO) regression analysis is used to analyze 17 possible influence factors;finally Logistic regression model is established to analyze and screen influence factors of risk. <strong>Results</strong>: 1) Among 158 elderly inpatients with medium and high risk of PI, the incidence of PI is 20.25%;the most common stage of injury is stage I (42.5%);sacrococcygeal (60%) is the high-risk site of pressure injury. 2) LASSO regression analysis shows that history of present respiratory infection/respiratory failure (<em>β </em>= 1.2714. <em>P</em> < 0.05) and hospitalization time (<em>β</em> = 0.4177. <em>P </em>< 0.05) are independent factors influencing PI risk in the elderly inpatients with kidney disease. <strong>Concl</strong><strong>usio</strong><strong>n</strong>: The elderly patients with kidney disease and PI risk are the high incidence population of hospital acquired PI;for the elderly inpatients with kidney disease and having respiratory infection history or respiratory failure, prolonged hospitalization will significantly increase the risk of PI. Therefore, targeted preventive and control measures should be taken to reduce the incidence of PI.
基金Supported by Liaoning S&T Project,No.20180550971 and No.20180550999Shenyang Young and Middle-Aged Scientific&Technological Innovation Talents Support Plan,No.2018416017.
文摘BACKGROUND Gastric cancer(GC)is one of the most frequently diagnosed gastrointestinal cancers throughout the world.Novel prognostic biomarkers are required to predict the prognosis of GC.AIM To identify a multi-long noncoding RNA(lncRNA)prognostic model for GC.METHODS Transcriptome data and clinical data were downloaded from The Cancer Genome Atlas.COX and least absolute shrinkage and selection operator regression analyses were performed to screen for prognosis associated lncRNAs.Receiver operating characteristic curve and Kaplan-Meier survival analyses were applied to evaluate the effectiveness of the model.RESULTS The prediction model was established based on the expression of AC007991.4,AC079385.3,and AL109615.2 Based on the model,GC patients were divided into“high risk”and“low risk”groups to compare the differences in survival.The model was re-evaluated with the clinical data of our center.CONCLUSION The 3-lncRNA combination model is an independent prognostic factor for GC.
文摘Background:Lung adenocarcinoma is one of the most common pathological types of lung malignant tumor with high morbidity and mortality.Long non-coding RNAs are gradually recognized to play crucial roles in tumor occurrence and development.Necroptosis is a newly established way for cell programmed death,undertaking essential roles in anti-tumor.Therefore,identifying necroptosis-related l ong non-coding RNAs and based on them to evaluate the signatures of l ung adenocarcinoma is essential for patients’survival prediction and therapy.Methods:We collected data from the public database and performed the least absolute shrinkage to construct a 13-lncRNAs prognostic model.Based on the Consensus Clustering,ESTIMATE,CIRERSORT,and weighted gene co-expression network analysis to identify the immune signatures.Results:This study identified a 13-lncRNAs prognostic model.The model’s prediction accuracy was evaluated by receiver operating characteristic and independent-prognosis analysis;besides,a Gene Expression Omnibus dataset was applied for external validation.Furthermore,we analyzed the immune features of subgroups in multiple dimensions.A consensus clustering analysis based on the 41 genes was implemented to separate lung adenocarcinoma patients into two subgroups.We compared the features of subgroups in multiple dimensions,including survival,immune microenvironment,immune cells infiltration and gene co-expression network analysis.Conclusion:W e established a prognosis necroptosis-related risk model to predict lung adenocarcinoma patients’prognosis and systematically understood the correlation between immune and necroptosis.This study can applicate in clinical to predict the prognosis of lung adenocarcinoma patients and provide new insight into lung adenocarcinoma immune therapy.
基金supported by the Medical Scientific Research Foundation of Guangdong Province(B2023012)the National Key R&D Program of China(Grant No.2020YFC2004705)+3 种基金the Research Unit of Medical Science Research Management/Basic and Clinical Research of Metabolic Cardiovascular Diseases from the Chinese Academy of Medical Sciences(Grant No.2021RU003)the National Natural Science Foundation of China(Grant Nos.81825003,91957123,81800327,81900272)Beijing Nova Program(Grant No.Z201100006820002)from the Beijing Municipal Science&Technology Commissionand the Science and Technology Project of Xicheng District Finance(Grant No.XCSTS-SD2021-01).
文摘Background:Dilated cardiomyopathy(DCM)has a high mortality rate and is the most common indication for heart transplantation.Our study sought to develop a multiparametric nomogram to assess individualized all-cause mortality or heart transplantation(ACM/HTx)risk in DCM patients.Methods:The present study is a retrospective cohort study.The demographic,clinical,blood test,and cardiac magnetic resonance imaging(CMRI)data of DCM patients in the tertiary center(Fuwai Hospital)were collected.The primary endpoint was ACM/HTx.The least absolute shrinkage and selection operator(LASSO)Cox regression model was applied for variable selection.Multivariable Cox regression was used to develop a nomogram.The concordance index(C-index),area under the receiver operating characteristic curve(AUC),calibration curve,and decision curve analysis(DCA)were used to evaluate the performance of the nomogram.Results:A total of 218 patients were included in the present study.They were randomly divided into a training cohort and a validation cohort.The nomogram was established based on eight variables,including mid-wall late gadolinium enhancement,systolic blood pressure,diastolic blood pressure,left ventricular ejection fraction,left ventricular end-diastolic diameter,left ventricular end-diastolic volume index,free triiodothyronine,and N-terminal pro-B type natriuretic peptide.The AUCs regarding 1-year,3-year,and 5-year ACM/HTx events were 0.859,0.831,and 0.840 in the training cohort and 0.770,0.789,and 0.819 in the validation cohort,respectively.The calibration curve and DCA showed good accuracy and clinical utility of the nomogram.Conclusions:We established and validated a circulating biomarker-and CMRI-based nomogram that could provide a personalized prediction of ACM/HTx for DCM patients,which might help risk stratification and decision-making in clinical practice.
基金co-supported by the Scientific Research Program of Tianjin Municipal Education Commission, China (No. 2021KJ042)the Special Project of the National Science Foundation of China (No. U2133204)。
文摘This paper proposes a robust vector tracking loop structure based on potential bias analysis. The influence of four kinds of biases on the existing two implementations of Vector Tracking Loops(VTLs) is illustrated by theoretical analysis and numerical simulations, and the following findings are obtained. Firstly, the initial user state bias leads to steady navigation solution bias in the relative VTL, while new measurements can eliminate it in the absolute VTL. Secondly, the initial code phase bias is transferred to the following navigation solutions in the relative VTL, while new measurements can eliminate it in the absolute VTL. Thirdly, the user state bias induced by erroneous navigation solution of VTLs can be eliminated by both of the two VTLs. Fourthly,the multipath/NLOS likely affects the two VTLs, and the induced tracking bias in the duration of the multipath/NLOS would decrease the performance of VTLs. Based on the above analysis,a robust VTL structure is proposed, where the absolute VTL is selected for its robustness to the two kinds of initialization biases;meanwhile, the instant bias detection and correction method is used to improve the performance of VTLs in the duration of the multipath/NLOS. Numerical simulations and experimental results verify the effectiveness of the proposed robust VTL structure.
基金the National Key R&D Program of China(Technology and application of wind power/photovoltaic power prediction for promoting renewable energy consumption,2018YFB0904200)eponymous Complement S&T Program of State Grid Corporation of China(SGLNDKOOKJJS1800266)。
文摘To enhance the performance of the prediction intervals (PIs), a novel very short-term probabilistic prediction method for wind speed via nonlinear quantile regression (NQR) based on adaptive least absolute shrinkage and selection operator (ALASSO) and integrated criterion (IC) is proposed. The ALASSO method is studied for shrinkage of output weights and selection of variables. Furthermore, for the better performance of PIs, composite weighted linear programming (CWLP) is proposed to modify the conventional linear programming cost function of quantile regression (QR), by combining it with Bayesian information criterion (BIC) as an IC to optimize the coefficients of PIs. Then, the multiple fold cross model (MFCM) is utilized to improve the PIs performance. Multistep probabilistic prediction of 15-minute wind speed is performed based on the real wind farm data from the northeast of China. The effectiveness of the proposed approach is validated through the performances' comparisons with conventional methods.
文摘Background:Utilizing population-based survey data in epidemiological research with a spatial perspective can integrate valuable context into the dynamics of HIV prevalence in West Africa.However,the situation in the Mano River Union(MRU)countries is largely unknown.This research aims to perform an ecological study to determine the HIV prevalence patterns in MRU.Methods:We analyzed Demographic and Health Survey(DHS)and AIDS Indicator Survey(AIS)data on HIV prevalence in MRU from 2005 to 2020.We examined the country-specifc,regional-specifc and sex-specifc ratios of respondents to profle the spatial–temporal heterogeneity of HIV prevalence and determine HIV hot spots.We employed Geodetector to measure the spatial stratifed heterogeneity(SSH)of HIV prevalence for adult women and men.We assessed the comprehensive correct knowledge(CCK)about HIV/AIDS and HIV testing uptake by employing the Least Absolute Shrinkage and Selection Operator(LASSO)regression to predict which combinations of CCKs can scale up the ratio of HIV testing uptake with sex-specifc needs.Results:In our analysis,we leveraged data for 158,408 respondents from 11 surveys in the MRU.From 2005–2015,Cote d’Ivoire was the hot spot for HIV prevalence with a Gi_Bin score of 3,Z-Score 8.0–10.1 and P<0.001.From 2016 to 2020,Guinea and Sierra Leone were hot spots for HIV prevalence with a Gi_Bin score of 2,Z-Score of 3.17 and P<0.01.The SSH confrmed the signifcant diferences in HIV prevalence at the national level strata,with a higher level for Cote d’Ivoire compared to other countries in both sexes with q-values of 0.61 and 0.40,respectively.Our LASSO model predicted diferent combinations of CCKs with sex-specifc needs to improve HIV testing uptake.Conclusions:The spatial distribution of HIV prevalence in the MRU is skewed and the CCK about HIV/AIDS and HIV testing uptake are far below the threshold target set by UNAIDS for ending the epidemic in the sub-region.Geodetector detected statistically signifcant SSH within and between countries in the MRU.Our LASSO model predicted that diferent emphases should be implemented when popularizing the CCK about HIV/AIDS for adult women and men.
