Absorption and photoluminescence spectroscopies are useful tools to study the photo-physical properties of materials. The theoretical methods for calculation of the spectra of molecules/supermolecules and aggregates, ...Absorption and photoluminescence spectroscopies are useful tools to study the photo-physical properties of materials. The theoretical methods for calculation of the spectra of molecules/supermolecules and aggregates, whose structures can differ significantly, are reviewed from the viewpoint of computational efficiency. Several model compounds/multimers are taken as examples for the spectral calculations. The numerical results achieve a satisfactory agreement between the theory and experiment.展开更多
Ratiometric probes facilitate quantitative studies via self-calibration and are cherished for bioimaging.Often,a small probe-product spectral separation leads to crosstalk,but the rational development of ratiometric p...Ratiometric probes facilitate quantitative studies via self-calibration and are cherished for bioimaging.Often,a small probe-product spectral separation leads to crosstalk,but the rational development of ratiometric probes with zero probe-product crosstalk remains challenging.Harnessing the recent progress on photophysical modulation of xanthenoid fluorochromes,we propose a powerful and versatile probe design principle,that is,“bridging-group modification,”and developed totalROX,a robust probe for monitoring the total cellular oxidative capacity.展开更多
基金supported by the National Natural Science Foundation of China (Grant Nos. 20673104, 20833003)the 973 project (Grant Nos. 2004CB719901 and 2006CB922004)
文摘Absorption and photoluminescence spectroscopies are useful tools to study the photo-physical properties of materials. The theoretical methods for calculation of the spectra of molecules/supermolecules and aggregates, whose structures can differ significantly, are reviewed from the viewpoint of computational efficiency. Several model compounds/multimers are taken as examples for the spectral calculations. The numerical results achieve a satisfactory agreement between the theory and experiment.
基金supported by the National Natural Science Foundation of China(nos.21822805,21908065,31871430,81802979,818800585,and 8180032537)Young Medical Talents Training Program of Shanghai(2018)+3 种基金the Commission of Science and Technology of Shanghai Municipality(no.18430711000)the Australian National Health and Medical Research Council(nos.APP1126091 and APP1125794)the Science and Technology Commission of Shanghai Municipality for the Shanghai International Cooperation Program(18430711000)the China Postdoctoral Science Foundation(nos.2019M651427 and 2020T130197),W.Yang acknowledges the financial support of the innovative research team of high-level local universities in Shanghai.
文摘Ratiometric probes facilitate quantitative studies via self-calibration and are cherished for bioimaging.Often,a small probe-product spectral separation leads to crosstalk,but the rational development of ratiometric probes with zero probe-product crosstalk remains challenging.Harnessing the recent progress on photophysical modulation of xanthenoid fluorochromes,we propose a powerful and versatile probe design principle,that is,“bridging-group modification,”and developed totalROX,a robust probe for monitoring the total cellular oxidative capacity.
