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普通菜豆(Phaseolus vulgaris L.)AOX基因家族鉴定与表达分析
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作者 张琦 王磊 +4 位作者 谷延华 周子善 王姝琨 徐俊杰 杜吉到 《黑龙江八一农垦大学学报》 2024年第2期1-8,共8页
交替氧化酶(Alternative oxidase,AOX)是一种末端氧化酶,在维持植物正常生长代谢速率和代谢平衡以及抵御逆境胁迫中发挥了一定作用。AOX家族成员已经在很多植物物种中被鉴定,但是在普通菜豆(Phaseolus vulgaris L.)中却未见报道。研究... 交替氧化酶(Alternative oxidase,AOX)是一种末端氧化酶,在维持植物正常生长代谢速率和代谢平衡以及抵御逆境胁迫中发挥了一定作用。AOX家族成员已经在很多植物物种中被鉴定,但是在普通菜豆(Phaseolus vulgaris L.)中却未见报道。研究对普通菜豆中AOX家族成员进行了鉴定和分析,并进一步解析了AOX基因家族成员的功能及调控机制。结果表明,基于普通菜豆参考基因组中共挖掘得到了4个AOX家族成员,通过进化关系、保守基序(Motifs)、基因结构分析,将各成员划分成4个亚族。此外,顺式作用元件、共线性与盐胁迫下的表达量分析结果表明,AOX成员可能在植物的非生物胁迫响应中发挥了作用。为后续深入研究普通菜豆中AOX成员的功能奠定了理论基础。 展开更多
关键词 普通菜豆(phaseolus vulgaris l.) 交替氧化酶 鉴定 生物信息学分析
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Response of Eight Market Classes of Dry Bean (<i>Phaseolus vulgaris</i>L.) to Pendimethalin
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作者 Nader Soltani Robert E. Nurse +1 位作者 Christy Shropshire Peter H. Sikkema 《American Journal of Plant Sciences》 2011年第6期835-840,共6页
There is little information on the tolerance of dry bean to pendimethalin. Field studies were conducted in 2007 to 2009 at Exeter, Ontario and in 2008 and 2009 at Ridgetown, Ontario to evaluate tolerance of black, cra... There is little information on the tolerance of dry bean to pendimethalin. Field studies were conducted in 2007 to 2009 at Exeter, Ontario and in 2008 and 2009 at Ridgetown, Ontario to evaluate tolerance of black, cranberry, kidney, otebo, pink, pinto, Small Red Mexican and white bean to the pendimethalin applied preplant incorporated at 1080 and 2160 g.a.i.ha-1. Pendimethalin PPI caused minimal injury in most market classes of dry bean at 1 and 2 WAE. There was no injury in various market classes of dry bean with the low dose at 1 and 2 weeks after emergence (WAE). However, at the high dose there was 0 to 4% injury at 1 WAE and 0 to 7% injury at 2 WAE in black, cranberry, kidney, otebo, pink, pinto, SRM and white bean. Pendimethalin PPI was more injurious in white bean than in black, cranberry, kidney, otebo, pink, pinto and SRM bean. Pink and SRM bean exhibited the most tolerance to pendimethalin applied PPI at 1080 g.ai.ha-1 or 2160 g.ai.ha-1. Pendimethalin caused no adverse effect on plant height, shoot dry weight, seed moisture content and seed yield of black, cranberry, kidney, otebo, pink, pinto, SRM and white bean. Based on these results, there is an adequate margin of crop safety for pendimethalin applied PPI at the proposed dose of 1080 g.ai.ha-1 in black, cranberry, kidney, otebo, pink, pinto, SRM and white bean in Ontario. 展开更多
关键词 Black bean CRANBERRY bean Kidney bean Otebo bean PENDIMETHAlIN Pink bean Small Red Mexican bean phaseolus vulgaris l. White bean
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Ultrastructural Analysis of the Ontogenetic Development of Shoot Induced from Embryonic Axes of Costa Rican Bean Varieties (<i>Phaseolus vulgaris</i>L.) under <i>in Vitro</i>Conditions by Scanning Electronic Microscopy
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作者 Marisol Jiménez Andrés Gatica +1 位作者 Ethel Sánchez Marta Valdez 《American Journal of Plant Sciences》 2012年第4期489-494,共6页
Common bean (Phaseolus vulgaris L.) is an economic important crop and one of the major grain legumes for human consumption in Latin America, Africa and Asia. A morphological study of shoot induced from embryonic axes ... Common bean (Phaseolus vulgaris L.) is an economic important crop and one of the major grain legumes for human consumption in Latin America, Africa and Asia. A morphological study of shoot induced from embryonic axes development in four Costa Rican bean varieties (Brunca, Huetar, Guaymi and Bribri) cultivated on MS media with or without 5 mg·L–1 de N6-benzylaminopurine (BAP) by scanning electron microscopy (SEM) was developed in the present work. Micrographs showed similarities and differences in the ultrastructure of the apical dome, epidermal surface, stomata and different types of trichomes in the varieties cultivated on organogenesis media. Genotypes with advantageous morphological characteristics for genetic transformation, in particular an exposed apical dome, were identified. This work will contribute to the optimization of the in vitro regeneration of four common bean varieties. 展开更多
关键词 phaseolus vulgaris l bean In Vitro ORGANOGENESIS SHOOT Scanning Electron Microscopy (SEM)
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Nutritional and Mineral Variability in 52 Accessions of Common Bean Varieties(Phaseolus vulgaris L.)from Madeira Island
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作者 Carla S.S.Gouveia Gregório Freitas +2 位作者 José H.de Brito Jan J.Slaski Miguel A.A.Pinheiro de Carvalho 《Agricultural Sciences》 2014年第4期317-329,共13页
The Phaseolus vulgaris L. is one of the traditional and most important leguminous crops in the Island of Madeira. The island’s bioclimatic tiers, agro-ecological environment and traditional farming practices had a gr... The Phaseolus vulgaris L. is one of the traditional and most important leguminous crops in the Island of Madeira. The island’s bioclimatic tiers, agro-ecological environment and traditional farming practices had a great influence on the evolution of regional bean landraces. The variability of the nutritional and mineral seeds composition of 59 accessions of the Madeiran landraces, standard and commercial varieties was evaluated. Wide ranges of variability in biochemical parameters were reported among the Madeiran landraces, being the best sources of protein and mineral nutrition, according to the statistical results and literature comparative evaluation. Specifically, the content (g per 100 g DW) of ash ranged from 3.64-5.67, lipids from 0.57-2.86, protein from 18.55-29.69, starch from 23.40-52.65, soluble sugars from 2.97-6.84, while content of dry matter was from 83.35-93.55. The seeds also contained (per 100 g DW) between 2.55-4.83 g N, 0.30-7.50 g P, 1.30-2.49 g K, 0.10-0.18 g Mg, 4.10-10.00 mg Fe, 50.0-1.40 mg Cu, 2.20-5.00 mg Zn, 0.90-3.80 μg Mn and 0.20-2.40 μg B. This variability implies that the screened germplasm could serve as a source for breeding new varieties with improved biochemical and nutritional traits or could be highly recommended to meet specific dietary requirements. The cultivar Vaginha Grossa (ISOP 713) revealed low carbohydrate content that could be a good food choice for diabetics, while cultivar Vermelho (ISOP 724) bean should be offered as a valuable alternative source of protein and minerals in the local diet. 展开更多
关键词 phaseolus vulgaris l. beanS Genetic Resources Proximal Analysis Nutritional Value Mineral Composition VARIABIlITY Food Quality
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Response of Four Market Classes of Dry Beans to Halosulfuron Applied Postemergence at Five Application Timings
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作者 Nader Soltani Christy Shropshire Peter H. Sikkema 《Agricultural Sciences》 2015年第2期247-255,共9页
Six field trials were conducted over a three-year period (2011, 2012, 2013) at the Huron Research Station, Exeter, Ontario and University of Guelph Ridgetown Campus, Ridgetown, Ontario to determine the effect of five ... Six field trials were conducted over a three-year period (2011, 2012, 2013) at the Huron Research Station, Exeter, Ontario and University of Guelph Ridgetown Campus, Ridgetown, Ontario to determine the effect of five postemergence (POST) application timings (1 - 2 trifoliate, 3 - 4 trifoliate, 5 - 6 trifoliate, 1st flower and 1st pod stage) of halosulfuron (35 and 70 g·ai·ha-1) on the tolerance of adzuki, black, white and kidney beans. All treatments including the non-treated control were maintained weed free during the growing season. Halosulfuron applied POST in black, white and kidney bean caused as much as 8%, 8%, 7%, 4% and 2% injury 1 WAA and 4%, 4%, 2%, 2% and 1% injury 2 WAA at 1 - 2 trifoliate, 3 - 4 trifoliate, 5 - 6 trifoliate, 1st flower and 1st pod stage application timings, respectively. The injury observed was transient with 1% or less injury 4 WAA and there was no adverse effect on the shoot dry weight, plant height, seed moisture content and yield of black, white and kidney bean. Injury was substantially higher in adzuki bean at all application timings. Halosulfuron applied POST caused as much as 66%, 47%, 50%, 39% and 36% injury 1 WAA;77%, 68%, 64%, 51% and 42% 2 WAA;and 69%, 51%, 47%, 40% and 29% 4 WAA at 1 - 2 trifoliate, 3 - 4 trifoliate, 5 - 6 trifoliate, 1st flower and 1st pod stage application timings, respectively. Halosulfuron POST decreased shoot dry weight as much as 69%, 57%, 43%, 41% and 34%;plant height as much as 17%, 15%, 14%, 13% and 10%;and seed yield as much as 47%, 46%, 45%, 56% and 55% at 1 - 2 trifoliate, 3 - 4 trifoliate, 5 - 6 trifoliate, 1st flower and 1st pod stages, respectively. Based on these results, there is an adequate margin of crop safety for halosulfuron POST in black, white and kidney beans. However, there is not an adequate margin of crop safety for halosulfuron POST in adzuki bean at the application timings evaluated. 展开更多
关键词 Adzuki bean Black bean KIDNEY bean White bean phaseolus vulgaris l.
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Weed Control with Sulfentrazone plus a Low Rate of Imazethapyr in White Bean
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作者 Allison N. Taziar Nader Soltani +4 位作者 Christy Shropshire Darren E. Robinson Mitch Long Chris L. Gillard Peter H. Sikkema 《Agricultural Sciences》 2016年第7期447-456,共10页
Ontario dry bean (Phaseolus vulgaris L.) growers have few options for broadleaf weed control. Sulfentrazone is a group 14 herbicide that provides good control of several common Ontario weed species, and would provide ... Ontario dry bean (Phaseolus vulgaris L.) growers have few options for broadleaf weed control. Sulfentrazone is a group 14 herbicide that provides good control of several common Ontario weed species, and would provide another mode of action for broadleaf weed control if registered for use in Ontario dry beans. Five field studies were conducted during 2014 and 2015 to determine if a low dose of imazethapyr added to a tank mix of sulfentrazone + s-metolachlor would improve broadleaf control in white bean. Sulfentrazone (140 and 210 g·ai·ha<sup>-1</sup>) was mixed with imazethapyr and s-metolachlor and evaluated at 2 and 4 weeks after crop emergence for crop injury. Weed control was assessed visually at 4 and 8 weeks after herbicide application (WAA), and weed stand counts and biomass were determined at 8 WAA. Seed moisture and yield were determined at harvest. At 8 WAA, sulfentrazone (140 g·ai·ha<sup>-1</sup>) controlled pigweed species, common ragweed, common lambsquarters, wild mustard, barnyard grass and green foxtail 100%, 4%, 100%, 2%, 86% and 62%, respectively. The addition of imazethapyr (37.5 g·ai·ha<sup>-1</sup>) to sulfentrazone (140 g·ai·ha<sup>-1</sup>) improved the control of common ragweed, wild mustard and green foxtail by 19%, 98% and 33%, respectively. The three-way tank mix of sulfentrazone (140 g·ai·ha<sup>-1</sup>) plus s-metolachlor (1050 g·ai·ha<sup>-1</sup>) plus imazethapyr (37.5 g·ai·ha<sup>-1</sup>) controlled pigweed species, common ragweed, common lambsquarters, wild mustard, barnyard grass and green foxtail 100%, 35%, 100%, 100%, 96% and 100%, respectively. The tank mixes evaluated caused unacceptably high levels of crop injury;this study does not support the registration of sulfentrazone plus s-metolachlor + imazethapyr for use in Ontario white bean. 展开更多
关键词 Biomass Density Injury HEIGHT Navy bean phaseolus vulgaris l.
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Broadleaf Weed Control with Halosulfuron Tankmixes in White Bean
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作者 Nader Soltani Lynette R. Brown Peter H. Sikkema 《American Journal of Plant Sciences》 2021年第7期1059-1072,共14页
Six field trials were conducted over a four-year (2017-2020) period near Exeter and Ridgetown, Ontario to determine the efficacy of halosulfuron tankmixes applied postemergence to control broadleaf weeds in white bean... Six field trials were conducted over a four-year (2017-2020) period near Exeter and Ridgetown, Ontario to determine the efficacy of halosulfuron tankmixes applied postemergence to control broadleaf weeds in white bean. Halosulfuron caused up to 4% injury in white bean at 2 and 4 weeks after treatment (WAT). Bentazon, acifluorfen, fomesafen, bentazon/acifluorfen, and bentazon + fomesafen caused 2% - 16% injury at 2 WAT and up to 3% injury at 4 WAT in white bean. The addition of halosulfuron to the aforementioned herbicides did not accentuate white bean injury. Reduced weed interference with the herbicides evaluated increased white been yield 50% - 90% compared to the weedy control;there was no difference in seed yield among herbicide treatments evaluated. At 4 WAT, halosulfuron at 25, 37.5 and 50 g ai ha-1 controlled velvetleaf 86%, 93% and 97%;redroot pigweed 83%, 85% and 89%;common ragweed 90%, 93% and 94%;common lambsquarters 27%, 28% and 36%;flower-of-an-hour 66%, 76% and 69%;and wild mustard 100%, 100% and 100%, respectively. Bentazon, acifluorfen, fomesafen, bentazon/acifluorfen, and bentazon + fomesafen controlled velvetleaf 73%, 14%, 52%, 42% and 68%;redroot pigweed 40%, 91%, 85%, 75% and 80%;common ragweed 36%, 81%, 92%, 68% and 84%;common lambsquarters 87%, 39%, 48%, 60% and 76%;flower-of-an-hour 90%, 66%, 63%, 73% and 83%;and wild mustard 97%, 97%, 100%, 99% and 100%, respectively. Halosulfuron tankmixed with bentazon, acifluorfen, fomesafen, bentazon/acifluorfen or bentazon + fomesafen controlled velvetleaf 90%, 51%, 68%, 75% and 90%;redroot pigweed 80%, 99%, 95%, 92% and 91%;common ragweed up to 94%, 97%, 93%, 94% and 95%;common lambsquarters 74%, 62%, 43%, 62% and 66%;flower-of-an-hour 92%, 78%, 74%, 82% and 87%;and wild mustard 100%, 100%, 100%, 100% and 100%, respectively. Weed density and dry biomass followed the same trend. This study concludes that the optimal halosulfuron tankmix is broadleaf weed species specific for weed management in dry bean production. 展开更多
关键词 Accentuated Injury MATURITY Broadleaf Weeds Yield White bean phaseolus vulgaris l.
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菜豆品种适应性比较试验 被引量:1
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作者 葛长军 张熔 +2 位作者 闫良 代俊芬 李进兰 《湖南农业科学》 2023年第11期10-13,共4页
为了筛选出适宜黄冈市不同栽培区域种植的菜豆品种,分别在黄冈市的平原和丘陵地区开展了菜豆品种适应性比较试验。结果表明:黄州(平原)试验点的产量以SJ-0009最高,其次是SJ-0007,分别为1295.3和1231.8 kg/667m^(2),分别比笔杆青(CK)增产... 为了筛选出适宜黄冈市不同栽培区域种植的菜豆品种,分别在黄冈市的平原和丘陵地区开展了菜豆品种适应性比较试验。结果表明:黄州(平原)试验点的产量以SJ-0009最高,其次是SJ-0007,分别为1295.3和1231.8 kg/667m^(2),分别比笔杆青(CK)增产22.9%和16.9%,且均显著高于其他品种,鄂菜豆1号的产量比CK增产5.4%;英山(丘陵)试验点的产量也是SJ-0009最高,为1155.6 kg/667m^(2),比CK增产17.4%,且显著高于除鄂菜豆1号以外的其他品种,其次是鄂菜豆1号和SJ-0007,分别比CK增产10.3%和3.9%,且均显著高于SJ-0361和江大紫芸1号;这3个品种的抗病性强,全生育期为91~94 d,系中晚熟品种,其果荚不易纤维化、口感脆甜,可溶性糖含量为0.06%,可在黄冈平原和丘陵地区推广种植。 展开更多
关键词 菜豆 品种 适应性 比较试验 黄冈市
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油豆角的涂膜保鲜研究 被引量:16
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作者 杨鑫 柳志强 王静 《食品科学》 EI CAS CSCD 北大核心 2003年第5期147-151,共5页
分析了油豆角(Phaseolus vulgaris L.)的变质原因,分离并初步鉴定了引起油豆角变质的病原菌,在此基础上确定了抑菌效果最佳的抑菌剂;研究了不同配方的壳聚糖保鲜剂对油豆角保鲜效果的影响。实验证明:在参试的五种药剂中,脱氢醋酸钠的抑... 分析了油豆角(Phaseolus vulgaris L.)的变质原因,分离并初步鉴定了引起油豆角变质的病原菌,在此基础上确定了抑菌效果最佳的抑菌剂;研究了不同配方的壳聚糖保鲜剂对油豆角保鲜效果的影响。实验证明:在参试的五种药剂中,脱氢醋酸钠的抑菌效果最为明显,可将其作为抑菌剂添加到保鲜剂中;选用2%的壳聚糖,0.2×10-6的脱氢醋酸钠和30×10-6的6-BA混合配制而成的保鲜剂,在12℃的环境下贮藏油豆角具有良好的效果。 展开更多
关键词 油豆角 涂膜保鲜 壳聚糖 保鲜剂 抑菌效果 病原菌 脱氢醋酸钠 贮藏
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壳聚糖可食用膜对油豆角贮藏生理的影响 被引量:12
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作者 连玉晶 赵海田 +3 位作者 姚磊 唐晓珍 邬元娟 王静 《食品与生物技术学报》 CAS CSCD 北大核心 2006年第3期72-74,共3页
使用壳聚糖配制的涂膜液处理油豆角,通过测定涂膜的油豆角在贮藏期间呼吸强度、PPO、POD、MDA的变化,探讨油豆角在贮藏期间的生理变化规律。结果表明,涂膜处理后能推迟油豆角呼吸高峰的到来;同时,在贮藏期间,涂膜油豆角的呼吸强度、PPO... 使用壳聚糖配制的涂膜液处理油豆角,通过测定涂膜的油豆角在贮藏期间呼吸强度、PPO、POD、MDA的变化,探讨油豆角在贮藏期间的生理变化规律。结果表明,涂膜处理后能推迟油豆角呼吸高峰的到来;同时,在贮藏期间,涂膜油豆角的呼吸强度、PPO、POD、MDA与对照组相比都有所降低。 展开更多
关键词 油豆角 壳聚糖 可食用膜
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可食性膜对油豆角贮藏期间品质的影响 被引量:12
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作者 杨鑫 柳志强 王静 《食品与发酵工业》 CAS CSCD 北大核心 2003年第8期44-47,共4页
采用正交实验设计配制保鲜剂 ,测定经处理的油豆角在贮藏期间水分及营养成分的变化 ,探讨油豆角贮藏期间品质的变化规律 ,结果表明 :随着贮藏时间的延长 ,油豆角中水分、Vc和还原糖的含量不断下降 ;叶绿素的含量初期下降 ,随后升高并出... 采用正交实验设计配制保鲜剂 ,测定经处理的油豆角在贮藏期间水分及营养成分的变化 ,探讨油豆角贮藏期间品质的变化规律 ,结果表明 :随着贮藏时间的延长 ,油豆角中水分、Vc和还原糖的含量不断下降 ;叶绿素的含量初期下降 ,随后升高并出现累积峰 ,最后又趋于降低。 展开更多
关键词 可食性膜 油豆角 贮藏期 保鲜剂 营养成分 变化规律 壳聚糖 涂膜保鲜
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白豆α-淀粉酶抑制剂糖蛋白的提取纯化及降血糖活性研究 被引量:25
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作者 张晓琦 杨明琰 +3 位作者 马瑜 田稼 宋纪蓉 戴德慧 《药物生物技术》 CAS CSCD 2007年第6期406-410,共5页
采用乙醇分级沉淀、CM纤维素离子交换柱层析及凝胶柱层析,从白豆中分离纯化得到一组分均一的白豆α-淀粉酶抑制剂(α-AI),其为一相对分子质量为36k的糖蛋白。通过对白豆α-淀粉酶抑制剂对四氧嘧啶高血糖模型大鼠空腹血糖及糖耐量的影响... 采用乙醇分级沉淀、CM纤维素离子交换柱层析及凝胶柱层析,从白豆中分离纯化得到一组分均一的白豆α-淀粉酶抑制剂(α-AI),其为一相对分子质量为36k的糖蛋白。通过对白豆α-淀粉酶抑制剂对四氧嘧啶高血糖模型大鼠空腹血糖及糖耐量的影响,研究其降血糖活性。当白豆α-AI使用剂量为150mg/kg体重,连续使用7d时,α-AI可明显降低高血糖大鼠的空腹血糖;使用剂量为300mg/kg时,对高血糖大鼠的糖耐量具有明显的改善作用。研究结果表明,从白豆中分离得到的淀粉酶抑制剂糖蛋白对高血糖大鼠具有明显的降血糖功能,其作为一种安全、天然的降糖药物具有良好的开发前景。 展开更多
关键词 白豆 Α-淀粉酶抑制剂 提取纯化 糖蛋白 降血糖活性
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壳聚糖及其衍生物对油豆角保鲜效果的研究 被引量:6
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作者 连玉晶 赵海田 +3 位作者 姚磊 唐晓珍 邬元娟 王静 《食品与发酵工业》 CAS CSCD 北大核心 2005年第9期106-109,共4页
分别用壳聚糖和羧甲基壳聚糖配制的涂膜液处理油豆角,比较不同处理组的生理指标及感官指标,确定保鲜效果较好的涂膜液。结果表明,涂膜处理的油豆角呼吸强度、多酚氧化酶和过氧化物酶活性及丙二醛含量与对照组相比都有所降低;用N,O羧甲... 分别用壳聚糖和羧甲基壳聚糖配制的涂膜液处理油豆角,比较不同处理组的生理指标及感官指标,确定保鲜效果较好的涂膜液。结果表明,涂膜处理的油豆角呼吸强度、多酚氧化酶和过氧化物酶活性及丙二醛含量与对照组相比都有所降低;用N,O羧甲基壳聚糖处理的油豆角保鲜效果较好。 展开更多
关键词 油豆角 壳聚糖 N O-羧甲基壳聚糖 可食用膜 羧甲基壳聚糖 保鲜效果 衍生物 过氧化物酶活性 丙二醛含量 多酚氧化酶
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微射流处理对芸豆分离蛋白构象和功能特性的影响 被引量:22
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作者 尹寿伟 唐传核 +1 位作者 温其标 杨晓泉 《华南理工大学学报(自然科学版)》 EI CAS CSCD 北大核心 2009年第10期112-116,123,共6页
采用高压微射流纳米均质机处理芸豆分离蛋白,运用溶解度、比浊法、热分析、荧光光谱、紫外光谱和圆二色光谱法探讨了不同压力微射流处理对芸豆分离蛋白构象和功能特性的影响.微射流处理诱导不溶性蛋白聚合物解聚,增加了蛋白质的溶解度,... 采用高压微射流纳米均质机处理芸豆分离蛋白,运用溶解度、比浊法、热分析、荧光光谱、紫外光谱和圆二色光谱法探讨了不同压力微射流处理对芸豆分离蛋白构象和功能特性的影响.微射流处理诱导不溶性蛋白聚合物解聚,增加了蛋白质的溶解度,改善了蛋白的乳化活性指数;圆二色光谱和荧光光谱分析表明,微射流处理对蛋白质的二级和三级结构没有明显的影响;热分析表明,蛋白热稳定性和有序结构均未受到显著的影响. 展开更多
关键词 芸豆蛋白 微射流 功能特性 构象
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菜豆几丁质酶基因Bchi的克隆及其在转基因烟草中的表达 被引量:22
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作者 王全伟 曲敏 +1 位作者 张海玲 徐香玲 《分子植物育种》 CAS CSCD 2008年第1期53-58,共6页
本文根据GenBank中公布的菜豆几丁质酶基因cDNA序列设计引物,通过RT-PCR得到一个菜豆几丁质酶基因家族基因Bchi。该基因编码一个327个氨基酸的多肽,其结构包括信号肽、几丁质结合区、铰链区、催化区和液泡定位多肽,属于ClassIa类内切几... 本文根据GenBank中公布的菜豆几丁质酶基因cDNA序列设计引物,通过RT-PCR得到一个菜豆几丁质酶基因家族基因Bchi。该基因编码一个327个氨基酸的多肽,其结构包括信号肽、几丁质结合区、铰链区、催化区和液泡定位多肽,属于ClassIa类内切几丁质酶。构建该基因的植物表达载体pMHL7133-Bchi,并通过发根农杆菌R1000介导转化烟草,经PCR和Southern blot鉴定获得了4株转基因烟草株系。几丁质酶活性分析表明,转基因烟草几丁质酶活力明显高于对照。抗真菌实验结果显示,转基因烟草的叶片基本无病斑产生,表明Bchi基因在烟草中高水平的表达显著提高了烟草对真菌病害的抗性。 展开更多
关键词 菜豆 几丁质酶 克隆和表达 烟草
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普通菜豆的起源、进化和遗传资源 被引量:19
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作者 严小龙 卢永根 《华南农业大学学报》 CAS CSCD 1994年第4期110-115,共6页
普通菜豆(PhaseolusvulgarisL.)是世界上重要的豆科作物之一,据研究,普通菜豆起源于两个独立的多样性中心,并分别在这两个中心被驯化成栽培植物,然后才逐渐被引人世界各地。在长期的驯化和栽培过程中,进化力... 普通菜豆(PhaseolusvulgarisL.)是世界上重要的豆科作物之一,据研究,普通菜豆起源于两个独立的多样性中心,并分别在这两个中心被驯化成栽培植物,然后才逐渐被引人世界各地。在长期的驯化和栽培过程中,进化力促使普通菜豆在形态、生理和遗传特性等方面发生了较大的演变,从而形成了丰富的遗传资源。本文就上述的研究进展作一简要综述,并就今后普通菜豆遗传资源的评价和利用工作提出一些见解。 展开更多
关键词 菜豆 起源 进化 遗传资源
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华南酸性红壤中菜豆种质耐低磷特性的评价 被引量:9
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作者 廖红 严小龙 《华南农业大学学报》 CAS CSCD 北大核心 1998年第2期20-25,共6页
以来源于两个不同基因库(安第斯基因库和中美洲基因库)的菜豆种质为材料,利用盆栽试验比较它们在华南地区酸性红壤上对低有效磷的适应性.结果表明,供试菜豆种质在耐低磷特性方面有基因型差异.利用聚类分析对所得结果进行磷效率分... 以来源于两个不同基因库(安第斯基因库和中美洲基因库)的菜豆种质为材料,利用盆栽试验比较它们在华南地区酸性红壤上对低有效磷的适应性.结果表明,供试菜豆种质在耐低磷特性方面有基因型差异.利用聚类分析对所得结果进行磷效率分类,发现MA和AN两个基因库均存在耐低磷能力和营养生长潜力的基因型,并且具有营养生长潜力的基因型一般不具有耐低磷性状.为此,必须鉴别出各种耐低磷基因型中与耐低磷有关的形态和生理生化指标,并分别找出与这些指标密切相关的基因,最后通过基因重组的方法培育理想的菜豆基因型. 展开更多
关键词 土壤 菜豆 基因型 低磷效率 聚类分析
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普通菜豆SSR反应条件的优化 被引量:5
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作者 冯国军 徐启江 +1 位作者 李玉花 刘大军 《东北农业大学学报》 CAS CSCD 2007年第1期27-34,共8页
在作物育种中,SSR是主要农艺性状分子标记及标记辅助育种的一项重要技术。以紫花、八月绿、将军、73-8、96-9、9z622等6个普通菜豆品种为试材,采用改良的SDS法提取了高质量的基因组DNA。为建立适宜的SSR反应体系,对影响SSR-PCR扩增的模... 在作物育种中,SSR是主要农艺性状分子标记及标记辅助育种的一项重要技术。以紫花、八月绿、将军、73-8、96-9、9z622等6个普通菜豆品种为试材,采用改良的SDS法提取了高质量的基因组DNA。为建立适宜的SSR反应体系,对影响SSR-PCR扩增的模板DNA浓度、Mg^(2+)的用量、Taq DNA聚合酶浓度、dNTPs浓度、退火温度等因素进行了优化。结果表明,在25μLPCR反应体系中,DNA模板的最适浓度为0.8ng·μL^(-1),Mg^(2+)的优化浓度为1.5mmol·L^(-1),Taq DNA聚合酶的最适浓度为0.05μmol·(μL·min)^(-1),dNTPs的最适浓度为0.2mmol·L^(-1)。 展开更多
关键词 普通菜豆 SSR 反应条件 优化
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黑龙江菜豆(油豆角)种质资源的收集整理与利用 被引量:8
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作者 詹云 郭牧 王雪 《中国蔬菜》 北大核心 2006年第B10期22-24,共3页
油豆角是以食用嫩荚为主的菜豆优良资源群。在黑龙江省收集的688份菜豆资源中,符合油豆角标准的有191份,占总数的27.8%。对不同栽培方式、不同生育期的油豆角资源特点进行了分析,并阐述了今后油豆角资源研究的方向。
关键词 黑龙江 油豆角 种质资源 收集和整理
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云南蔓生型普通菜豆资源形态学遗传多样性分析 被引量:3
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作者 代程 何玉华 +4 位作者 包世英 王丽萍 杨峰 于海天 吕梅媛 《西南农业学报》 CSCD 北大核心 2017年第2期256-261,共6页
文章对129份云南地方蔓生型普通菜豆种质资源进行了农艺性状的遗传多样性分析。结果表明,在云南地区的普通菜豆种质资源在形态学上具有丰富的多样性。10个农艺性状的平均变异系数为0.24,其中以单株荚数的变异度最大,达到0.61。平均多样... 文章对129份云南地方蔓生型普通菜豆种质资源进行了农艺性状的遗传多样性分析。结果表明,在云南地区的普通菜豆种质资源在形态学上具有丰富的多样性。10个农艺性状的平均变异系数为0.24,其中以单株荚数的变异度最大,达到0.61。平均多样性指数为2.04,各性状间数值差异不大。通过因子分析中的主成分法将10个变量降维获得4个公共因子,含义清晰且解释度较好。利用公共因子得分进行聚类分析,129份菜豆资源可分为2大类5个亚群。其特征是:亚群Ⅰ表现为分枝及结荚多,单荚粒数多,百粒重较小而单株产量高;亚群Ⅱ表现晚熟,荚小,百粒重和单株产量低;亚群Ⅲ表现长荚;亚群Ⅳ资源数量最多,表现分枝少,较早熟;亚群Ⅴ表现为早熟,结荚少,荚型短宽且单荚粒数少,百粒重大。 展开更多
关键词 普通菜豆 种质资源 遗传多样性 云南
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