Larix olgensis A. Henry(Changbai larch) is a productive commercial species and good candidate for afforestation in northeast China. It is widely planted in lead-stressed soils which can induce oxidative damage in this...Larix olgensis A. Henry(Changbai larch) is a productive commercial species and good candidate for afforestation in northeast China. It is widely planted in lead-stressed soils which can induce oxidative damage in this plant. Increasing tolerance to lead(Pb) stress is therefore of keen interest. A greenhouse experiment was conducted to identify the biomass, physiological responses and Pb accumulation of L. olgensis seedlings to Pb stress under succinic acid(SA) application and to explore the interaction of exogenous SA applications and stress resistance. L. olgensis seedlings were planted in Pb-stressed or unstressed haplic cambisols in pots. In Pb-contaminated soils the seedlings were treated daily with concentrations of SA solutions at a rate approximately equivalent to 0, 0.04,0.2, 1.0, or 2.0 mmol kg-1of soil for 10, 20, and 30 days,respectively. Pb treatment induced damage in the seedlings and led to the inhibition of biomass accumulation in roots,stems and leaves, and a rise in Pb accumulation in fine roots and leaves. Malondialdehyde(MDA) content and electrolyte leakage in leaves significantly increased while peroxidase(POD) activities, soluble protein and photosynthetic pigment contents in leaves were all reduced.Physiological toxicity was promoted with increasing Pb treatment times. When Pb-stressed seedlings were exposed to SA(especially 10.0 mmol L-1 over 20 days), the physiological responses for Pb-only were reversed and the biomass of roots, stems, and leaves dramatically increased.SA facilitated Pb uptake in fine roots and leaves but more Pb accumulated in fine roots. The results demonstrate that exogenous SA alleviates Pb-induced oxidative injuries and improves the tolerance of L. olgensis seedlings to Pb stress.展开更多
为缓解重金属Pb对烤烟的胁迫,降低Pb对烤烟的毒害作用,采用盆栽试验模拟土壤Pb污染(200 mg/kg),设置不施肥(CK)、单施烟草专用复合肥(T1)、烟草专用复合肥+生物炭配施(T2)、烟草专用复合肥+生物炭+有机肥配施(T3)4种处理,研究了Pb胁迫...为缓解重金属Pb对烤烟的胁迫,降低Pb对烤烟的毒害作用,采用盆栽试验模拟土壤Pb污染(200 mg/kg),设置不施肥(CK)、单施烟草专用复合肥(T1)、烟草专用复合肥+生物炭配施(T2)、烟草专用复合肥+生物炭+有机肥配施(T3)4种处理,研究了Pb胁迫下生物炭和有机肥配施对烤烟生物量积累、烟株根系活力、烟株Pb积累和土壤微生物数量的影响。结果表明,移栽后90 d,T3处理烤烟生物量比T1处理提高24.5%,且在相同施氮量情况下T3>T2>T1;烟株根系活力总体表现为T3>T2>T1>CK,其中,移栽后90 d T3、T2处理较CK分别高94.9%、55.1%;移栽后90 d T3处理土壤中有效态Pb、烟茎Pb含量和烟叶Pb含量分别较CK降低15.8%、40.6%和25.0%,另外,T3处理烟叶Pb含量与T1、T2处理相比分别下降了30.0%、20.9%;T3处理在土壤pH值和土壤微生物数量方面与CK和T1处理相比有显著改善。总体上,施用生物炭以及生物炭和有机肥配施可以降低重金属Pb胁迫对烤烟生长发育的影响,降低土壤有效态Pb含量,减少Pb在根系、茎、叶中的积累,提高土壤微生物数量。展开更多
基金financially supported by the National Natural Science Foundation of China(31370613)the Fundamental Research Funds for the Central Universities(2572017CA03)
文摘Larix olgensis A. Henry(Changbai larch) is a productive commercial species and good candidate for afforestation in northeast China. It is widely planted in lead-stressed soils which can induce oxidative damage in this plant. Increasing tolerance to lead(Pb) stress is therefore of keen interest. A greenhouse experiment was conducted to identify the biomass, physiological responses and Pb accumulation of L. olgensis seedlings to Pb stress under succinic acid(SA) application and to explore the interaction of exogenous SA applications and stress resistance. L. olgensis seedlings were planted in Pb-stressed or unstressed haplic cambisols in pots. In Pb-contaminated soils the seedlings were treated daily with concentrations of SA solutions at a rate approximately equivalent to 0, 0.04,0.2, 1.0, or 2.0 mmol kg-1of soil for 10, 20, and 30 days,respectively. Pb treatment induced damage in the seedlings and led to the inhibition of biomass accumulation in roots,stems and leaves, and a rise in Pb accumulation in fine roots and leaves. Malondialdehyde(MDA) content and electrolyte leakage in leaves significantly increased while peroxidase(POD) activities, soluble protein and photosynthetic pigment contents in leaves were all reduced.Physiological toxicity was promoted with increasing Pb treatment times. When Pb-stressed seedlings were exposed to SA(especially 10.0 mmol L-1 over 20 days), the physiological responses for Pb-only were reversed and the biomass of roots, stems, and leaves dramatically increased.SA facilitated Pb uptake in fine roots and leaves but more Pb accumulated in fine roots. The results demonstrate that exogenous SA alleviates Pb-induced oxidative injuries and improves the tolerance of L. olgensis seedlings to Pb stress.
文摘为缓解重金属Pb对烤烟的胁迫,降低Pb对烤烟的毒害作用,采用盆栽试验模拟土壤Pb污染(200 mg/kg),设置不施肥(CK)、单施烟草专用复合肥(T1)、烟草专用复合肥+生物炭配施(T2)、烟草专用复合肥+生物炭+有机肥配施(T3)4种处理,研究了Pb胁迫下生物炭和有机肥配施对烤烟生物量积累、烟株根系活力、烟株Pb积累和土壤微生物数量的影响。结果表明,移栽后90 d,T3处理烤烟生物量比T1处理提高24.5%,且在相同施氮量情况下T3>T2>T1;烟株根系活力总体表现为T3>T2>T1>CK,其中,移栽后90 d T3、T2处理较CK分别高94.9%、55.1%;移栽后90 d T3处理土壤中有效态Pb、烟茎Pb含量和烟叶Pb含量分别较CK降低15.8%、40.6%和25.0%,另外,T3处理烟叶Pb含量与T1、T2处理相比分别下降了30.0%、20.9%;T3处理在土壤pH值和土壤微生物数量方面与CK和T1处理相比有显著改善。总体上,施用生物炭以及生物炭和有机肥配施可以降低重金属Pb胁迫对烤烟生长发育的影响,降低土壤有效态Pb含量,减少Pb在根系、茎、叶中的积累,提高土壤微生物数量。
