Vacuolar Phosphate Transporter1(VPT1)-mediated phosphate uptake in the vacuoles is essential to plant development and fruit ripening.Interestingly,here we find that the VPT1 may transport sugar in response to soluble ...Vacuolar Phosphate Transporter1(VPT1)-mediated phosphate uptake in the vacuoles is essential to plant development and fruit ripening.Interestingly,here we find that the VPT1 may transport sugar in response to soluble sugar status of fruits.The VvVPT1 protein isolated from grape(Vitis vinifera)berrieswas tonoplast-localized and contains SPX(Syg1/Pho81/XPR1)and MFS(major facilitator superfamily)domains.Its mRNA expression was significantly increased during fruit ripening and induced by sucrose.Functional analyses based on transient transgenic systems in grape berry showed that VvVPT1 positively regulated berry ripening and significantly affected hexose contents,fruit firmness,and ripening-related gene expression.The VPT1 proteins(Grape VvVPT1,strawberry FaVPT1,and Arabidopsis AtVPT1)all showed low affinity for phosphate verified in yeast system,while they appear different in sugar transport capacity,consistent with fruit sugar status.Thus,our findings reveal a role for VPT1 in fruit ripening,associated to its SPX and MFS domains in direct transport of soluble sugar available into the vacuole,and open potential avenues for genetic improvement in fleshy fruit.展开更多
Vaccinium duclouxii,endemic to southwestern China,is a berry-producing shrub or small tree belonging to the Ericaceae family,with high nutritive,medicinal,and ornamental value,abundant germplasm resources,and good edi...Vaccinium duclouxii,endemic to southwestern China,is a berry-producing shrub or small tree belonging to the Ericaceae family,with high nutritive,medicinal,and ornamental value,abundant germplasm resources,and good edible properties.In addition,V.duclouxii exhibits strong tolerance to adverse environmental conditions,making it a promising candidate for research and offering wide-ranging possibilities for utilization.However,the lack of V.duclouxii genome sequence has hampered its development and utilization.Here,a high-quality telomere-to-telomere genome sequence of V.duclouxii was de novo assembled and annotated.All of 12 chromosomes were assembled into gap-free single contigs,providing the highest integrity and quality assembly reported so far for blueberry.The V.duclouxii genome is 573.67 Mb,which encodes 41953 protein-coding genes.Combining transcriptomics and metabolomics analyses,we have uncovered the molecular mechanisms involved in sugar and acid accumulation and anthocyanin biosynthesis in V.duclouxii.This provides essentialmolecular information for further research on the quality of V.duclouxii.Moreover,the high-quality telomere-to-telomere assembly of the V.duclouxii genome will provide insights into the genomic evolution of Vaccinium and support advancements in blueberry genetics and molecular breeding.展开更多
Soluble sugar content in seeds is an important quality trait of soybean. In this study, 57 quantitative trait loci(QTLs) related to soluble sugar contents in soybean seeds were collected from databases and published p...Soluble sugar content in seeds is an important quality trait of soybean. In this study, 57 quantitative trait loci(QTLs) related to soluble sugar contents in soybean seeds were collected from databases and published papers. After meta-overview-collinearity integrated analysis to refine QTL intervals, eight consensus QTLs were identified. To further verify the consensus QTLs, a population of chromosome segment substitution lines(CSSLs) was analyzed. Two lines containing fragments covering the regions of consensus QTLs and the recurrent parent were selected: one line showed high soluble sugar contents associated with a consensus QTL fragment, and the other line showed low soluble sugar contents. Transcriptome sequencing was conducted for these two lines at the early, middle, and late stages of seed development, which identified 158, 109 and 329 differentially expressed genes, respectively. Based on the analyses of re-sequencing data of the CSSLs and the consensus QTL region, three candidate genes(Glyma.19 G146800, Glyma.19 G122500, and Glyma.19 G128500) were identified in the genetic fragments introduced from wild soybean. Sequence comparisons between the two CSSL parents SN14 and ZYD00006 revealed a single nucleotide polymorphism(SNP) mutation in the coding sequence of Glyma.19 G122500, causing a nonsynonymous mutation in the amino acid sequence that affected the predicted protein structure. A Kompetitive allele-specific PCR(KASP) marker was developed based on this SNP and used to evaluate the CSSLs. These results lay the foundation for further research to identify genes related to soluble sugar contents in soybean seeds and for future soybean breeding.展开更多
Soluble sugar is a key quality trait of soybean seeds.We developed rapid and economic extraction and quantification methods for seed soluble sugars using an ultra-high performance liquid chromatography system with a r...Soluble sugar is a key quality trait of soybean seeds.We developed rapid and economic extraction and quantification methods for seed soluble sugars using an ultra-high performance liquid chromatography system with a refractive index detector.We evaluated the soluble sugar compositions of 1164 soybean accessions collected from diverse ecoregions and grown in multiple locations and years.Total soluble sugar(TSS)content was influenced by accession type,year of cultivation,and ecoregion.The mean contents of fructose,glucose,sucrose,raffinose,stachyose and TSS were 3.31,5.21,55.60,6.60,35.47,and 106.19 mg g^(-1),respectively.The highest mean TSS content(108.71 mg g^(-1)) was observed in accessions from Northern Region of China.Cultivars contained higher contents of sucrose,raffinose,and TSS,whereas landraces had a higher content of stachyose.Fourteen accessions with mean TSS contents>130 mg g^(-1) were identified as elite soybean resources.TSS was correlated with sucrose,raffinose,stachyose,protein,oil and total tocopherol.The main soluble sugar components were correlated with latitude and longitude,indicating that the geographical origin of the accessions affected their seed soluble sugar compositions.The developed methods and elite identified accessions can be used in the food and feed industry and in soybean breeding programs aimed at improving soybean seed nutrition.展开更多
Liaoyuanduoli tomato was planted in pots in a solar greenhouse. The first leaf below the second cluster was labeled by14CO2 with a dose of 0.555 MBq 30 days after the second cluster anthesis. Mesophyll, midrib, petiol...Liaoyuanduoli tomato was planted in pots in a solar greenhouse. The first leaf below the second cluster was labeled by14CO2 with a dose of 0.555 MBq 30 days after the second cluster anthesis. Mesophyll, midrib, petiole vascular of the fedleaf, internode vascular below the fed leaf (adjacent to the fed leaf), peduncle vascular of the second cluster, sepal, fruitpedicle, pulp, pectinic, and dissepiment were taken 2, 4, 8, 12, 20, 24, 48, and 72 h, respectively, after 14CO2 feed todeterminate the composition and specific activity of carbohydrate at different sites and different time after the labeling.The results indicated that almost all of the photosynthates resulting from 1 h of photosynthesis could be transported outof the leaf within 72 h and more than 85% of them were exported within 24 h. The greatest amount of sucrose transportoccurred in the internode pedicel vascular bundle 8 h after the start of photosynthesis. Assimilates entering the fruitthrough the internode and pedicel vascular bundles were detectable but in small quantities 2 h after photosynthesis. Onlythe radio activity of sucrose but not that of glucose and fructose was detected, suggesting that some of the sucrose in thefruit came directly through the phloem at an early stage of fruit development, not through the synthesis of glucose andfructose. Fruit vascular may be the part where sucrose first metabolized after it entered the fruit.展开更多
The content of soluble sugar in cowpea(Vigna unguiculata L. Walp.) seeds is important for their acceptability to consumers. The objective of this study was to evaluate the genetic control of soluble sugar content in c...The content of soluble sugar in cowpea(Vigna unguiculata L. Walp.) seeds is important for their acceptability to consumers. The objective of this study was to evaluate the genetic control of soluble sugar content in cowpea using diallel analysis. For this purpose,half-diallel crosses were made among seven diverse parents. The amount of total soluble sugar was estimated by the phenol sulfuric acid reagent method and reducing sugar were measured by the DNS(3,5-dinitrosalicylic acid) reagent method. The total soluble sugar ranged from 11.12(VYA) to 40.79 mg g^(-1)(IT93 K-693-2) with a mean of 22.31 mg g^(-1).Reducing soluble sugar showed a mean value of 9.11 mg g^(-1) and ranged from 13.61(TVx3236) to 4.98 mg g^(-1)(Lori niebe). Diallel analysis showed that both additive and non-additive gene effects were significant in the genetic control of these traits. However,dominance variance was greater than additive variance for soluble sugar. The traits were controlled mainly by an overdominance model, suggesting the advantage of delaying selection to later generations. Soluble sugar content was found to be highly heritable, with broad-sense heritability ranging from 0.987 to 0.976. Narrow-sense heritability ranged from0.154 to 0.422 and revealed the lower importance of additive variance. Parents and F_1 hybrids differed significantly on the basis of their general and specific combining abilities,respectively. Recessive alleles had positive effects on total soluble sugar and reducing soluble sugar. These results could help cowpea breeders improve the acceptability of cowpea seeds and cowpea-based foods by appropriate selection.展开更多
High temperature stress on different varieties of lettuce seedlings in MDA, proline and soluble sugar content were studied. The results were as follows : With the stress temperature, varieties of leaf lettuce seedling...High temperature stress on different varieties of lettuce seedlings in MDA, proline and soluble sugar content were studied. The results were as follows : With the stress temperature, varieties of leaf lettuce seedlings in the MDA, proline and soluble sugar content gradually increased;Different varieties had the most significant difference in 38/33℃(d/n). The increase of heat-resistant varieties on proline and soluble sugar content was quick and high, while its increase in MDA content was slow and small.展开更多
Alkali stress can cause severe crop damage and reduce production. However, physiological processes involved in alkali stress in oat seedlings are not well understood. In this study, physiological responses and yield o...Alkali stress can cause severe crop damage and reduce production. However, physiological processes involved in alkali stress in oat seedlings are not well understood. In this study, physiological responses and yield of oat to alkali stress were studied using the alkali-tolerant oat genotype Vao-9 and the alkali-sensitive oat genotype Baiyan 5. The results were: (i) low concentrations of alkali stress (25 and 50 mmol L-1) significantly reduced the yield and grain weight while increased the oat grain number per spike. A negative correlation between yield and malondialdehyde (MDA) content at the jointing and grain filling stages and positive correlations between yield on one hand and superoxide dismutase (SOD), and peroxidase (POD) activities on the other at the jointing stage were observed. There was a positive correlation between MDA and soluble sugar at the grain filling stage; (ii) soluble sugar content was increased at the jointing and grain filling stages and decreased at the heading stage by alkali stress; (iii) alkali stress increased the SOD activity during the heading and grain filling stages, and increased the POD activity at the heading stage. As compared to the control, the increase of MDA contents in alkali-treated oat was observed, during the jointing, heading and grain filling stages; (iv) under alkali stress, the oat genotype Vao-9 showed higher antioxidant enzyme activity and lower soluble sugar contents during the heading stage, and lower MDA contents than those in the oat genotype Baiyan 5 under alkali stress. The result suggested that the high ROS scavenging capacity and soluble sugar levels might play roles in oat response to alkali stress.展开更多
Cowpea (Vigna unguiculata (L.) Walp) is a legume crop grown worldwide to provide protein, starch, soluble sugar, amino acids, fatty acids, minerals, and vitamins for human consumption and animal feed. Soluble sugar is...Cowpea (Vigna unguiculata (L.) Walp) is a legume crop grown worldwide to provide protein, starch, soluble sugar, amino acids, fatty acids, minerals, and vitamins for human consumption and animal feed. Soluble sugar is an important physiological trait in cowpea seeds. It not only plays an important role in storability and abiotic stress tolerance in seeds, but also provides energy to the human body and improves cooking quality by giving a desirable taste and mouth feel. Therefore, this research aimed to evaluate variation of cowpea seed soluble sugar content, and assess the soluble sugar content of cowpea varieties with different seed coat colors for cowpea consumers. A total of 113 cowpea genotypes were used, including 89 USDA GRIN germplasm accessions and 24 advanced breeding lines developed from University of Arkansas, AR, USA. Seed soluble sugar content was measured using a Spectrophotometer by phenol-sulphuric acid method. The results showed that the seed soluble sugar contents among 113 cowpea genotypes exhibited a wide range varying from 32.6 mg/g to 86.1 mg/g with an average of 54.5 mg/g. The five highest soluble sugar contents in seeds were found in the Arkansas cultivar Empire (86.1 mg/g), USDA germplasm accession PI583202 (84.5 mg/g), Arkansas advanced breeding line 09-655 (82.1 mg/g), USDA accession PI601085 (81.6 mg/g), and Arkansas advanced breeding line 09-529 (80.9 mg/g), and they can be used in cowpea breeding programs to develop new cowpea cultivars with higher seed soluble sugar content. It was also observed that the soluble sugar contents in Arkansas commercial cultivars and advanced breeding lines were higher than those in USDA germplasm and the seeds with colorful coat may have soluble sugar levels desirable by consumers.展开更多
Two rice maintaining lines with different drought tolerances, viz., Jin 23B (tolerant) and Zhenshan 97B (sensitive), were used to study the oxidative stress and soluble sugar in rice anthers and pollen viability under...Two rice maintaining lines with different drought tolerances, viz., Jin 23B (tolerant) and Zhenshan 97B (sensitive), were used to study the oxidative stress and soluble sugar in rice anthers and pollen viability under drought stress during flowering stage. Higher antioxidant enzyme activities and lower malonaldehyde (MDA) content in rice anthers were observed in Jin 23B than in Zhenshan 97B under drought stress. Further, a great increase in the content of soluble sugar in rice anthers of Jin 23B was observed across the whole drought exposure, while Zhenshan 97B showed significant decrease in soluble sugar during 9-12 d after drought stress (DADS). Accordingly, a marked decline of pollen fertility and activity, pollen numbers in an anther and pollen numbers on a stigma was observed in Zhenshan 97B, whereas little difference was found in Jin 23B. Thus, we suggest that pollen abortion caused by drought stress may be related with the reciprocity between oxidative stress and soluble sugar content in rice anthers.展开更多
Sugar from plant photosynthesis is a basic requirement for life activities.Sugar transporters are the proteins that mediate sugar allocation among or within source/sink organs.The transporters of the major facilitator...Sugar from plant photosynthesis is a basic requirement for life activities.Sugar transporters are the proteins that mediate sugar allocation among or within source/sink organs.The transporters of the major facilitator superfamily(MFS)targeting carbohydrates represent the largest family of sugar transporters in many plants.Strawberry(Fragaria×ananassa Duchesne)is an important crop appreciated worldwide for its unique fruit flavor.The involvement of MFS sugar transporters(STs)in cultivated strawberry fruit sugar accumulation is largely unknown.In this work,we characterized the genetic variation associated with fruit soluble sugars in a collection including 154 varieties.Then,a total of 67 ST genes were identified in the v4.0 genome integrated with the v4.0.a2 protein database of F.vesca,the dominant subgenome provider for modern cultivated strawberry.Phylogenetic analysis updated the nomenclature of strawberry ST homoeologs.Both the chromosomal distribution and structural characteristics of the ST family were improved.Semi-RT-PCR analysis in nine tissues from cv.Benihoppe screened 34 highly expressed ST genes in fruits.In three varieties with dramatically differing fruit sugar levels,qPCR integrated with correlation analysis between ST transcript abundance and sugar content identified 13 sugar-correlated genes.The correlations were re-evaluated across 19 varieties,including major commercial cultivars grown in China.Finally,a model of the contribution of the sugar transporter system to subcellular sugar allocation in strawberry fruits was proposed.Our work highlights the involvement of STs in controlling strawberry fruit soluble sugars and provides candidates for the future functional study of STs in strawberry development and responses and a new approach for strawberry genetic engineering and molecular breeding.展开更多
There are different levels of resistance in Chinese cabbage varieties against clubroot.The content of catalase(CAT),peroxidase(POD),phenylalanin ammonia-lyase(PAL),Super Oxide Dismutase(SOD)and soluble sugar activitie...There are different levels of resistance in Chinese cabbage varieties against clubroot.The content of catalase(CAT),peroxidase(POD),phenylalanin ammonia-lyase(PAL),Super Oxide Dismutase(SOD)and soluble sugar activities of 3cultivars with different clubroot-resistant levelswere detected after inoculation in this study.The results suggest that the changing rates of CAT,PAL and SOD contents of resistance cultivars after inoculation were higher than those of susceptible cultivars(Baigengbaiye>Zaobaicaitai>Huangjinxiaobaicai);the POD activities of resistance cultivars reached a peak on an earlier day than those of susceptible cultivars;the increasing rate of soluble sugar of susceptible cultivars was higher than that of the resistance cultivars,and ended with two peaks.Therefore,the four enzymes(CAT,POD,PAL and SOD)and soluble sugar may be used as physiological and biochemical reference indexes for the resistance identification to clubroot after inoculation with P.brassicae.展开更多
Drought is a major production constraint for major fruits and vegetable crops in the tropics. This study was conducted to investigate the effect of limited water supply at three growth stages(vegetative, flowering and...Drought is a major production constraint for major fruits and vegetable crops in the tropics. This study was conducted to investigate the effect of limited water supply at three growth stages(vegetative, flowering and fruiting) on the accumulation of proline and soluble sugars in three pepper species. Seeds of the three pepper species, Capsicum chinense Jacq., C. annuum L. and C. frutescens L. were raised in a nursery and the seedlings were transplanted into seventy two plastic pots arranged in a randomized complete block design with three replicates, 25 days after planting. Four water treatments, 200 m L of water supplied twice daily(W1), once in every three days(W2), once in every five days(W3), and zero water supplied throughout growing period(W0) were imposed at three vegetative, flowering, and fruiting growth stages. Data were collected on relative water content, free proline and total soluble sugar. Data collected were subjected to analysis of variance and means were separated using Duncan's multiple range test. Results show that the concentration of proline and soluble sugar in leaves of the three pepper species were found to be remarkable at the different stages of growth in the stressed plants.展开更多
Orphan genes(OGs),which are genes unique to a specific taxon,play a vital role in primary metabolism.However,little is known about the functional significance of Brassica rapa OGs(BrOGs)that were identified in our pre...Orphan genes(OGs),which are genes unique to a specific taxon,play a vital role in primary metabolism.However,little is known about the functional significance of Brassica rapa OGs(BrOGs)that were identified in our previous study.To study their biological functions,we developed a BrOG overexpression(BrOGOE)mutant library of 43 genes in Arabidopsis thaliana and assessed the phenotypic variation of the plants.We found that 19 of the 43 BrOGOE mutants displayed a mutant phenotype and 42 showed a variable soluble sugar content.One mutant,BrOG1OE,with significantly elevated fructose,glucose,and total sugar contents but a reduced sucrose content,was selected for indepth analysis.BrOG1OE showed reduced expression and activity of the Arabidopsis sucrose synthase gene(AtSUS);however,the activity of invertase was unchanged.In contrast,silencing of two copies of BrOG1 in B.rapa,BraA08002322(BrOG1A)and BraSca000221(BrOG1B),by the use of an efficient CRISPR/Cas9 system of Chinese cabbage(B.rapa ssp.campestris)resulted in decreased fructose,glucose,and total soluble sugar contents because of the upregulation of BrSUS1b,BrSUS3,and,specifically,the BrSUS5 gene in the edited BrOG1 transgenic line.In addition,we observed increased sucrose content and SUS activity in the BrOG1 mutants,with the activity of invertase remaining unchanged.Thus,BrOG1 probably affected soluble sugar metabolism in a SUS-dependent manner.This is the first report investigating the function of BrOGs with respect to soluble sugar metabolism and reinforced the idea that OGs are a valuable resource for nutrient metabolism.展开更多
Alternative and renewable bio-based energy sources are gaining prominence worldwide. Sweet sorghum is currently being evaluated throughout the world because its stem juices are rich in sugars that can be directly ferm...Alternative and renewable bio-based energy sources are gaining prominence worldwide. Sweet sorghum is currently being evaluated throughout the world because its stem juices are rich in sugars that can be directly fermented to ethanol. In this two-year study, sweet sorghum varieties;Dale, Theis, Topper 76-6, and M81E (Obtained from Mississippi State University Experiment Station, MS) and CHR-SW8 (Obtained from Chromatin Inc., IL) were used. Nitrogen (N) fertilizer rates of 0, 40, 80 or 120 kg·N·ha-1 were applied to experimental units. The experiment was a randomized complete block design with treatments in a split-split plot arrangement with three replications. Nitrogen rate was the main plot, cultivar as sub-plot, and panicle removal as sub-plot. Results showed that N application increased fresh stem yield, juice volume, but had minimal effect on juice soluble sugar concentration. Compared to controls, application of ≥40 kg·N·ha-1 increased fresh yield and juice by >60% and 10%, respectively. There were also variety differences in harvested fresh biomass, juice volume and oBrix, and soluble sugar content. Dale and Theis consistently showed lower sucrose compared to other varieties over the two years. Panicle removal during early reproductive phase increased oBrix, sucrose and total sugar content in all varieties. Across the two years of study, panicle removal increased oBrix by more than 10%, sucrose and total sugar increased by more than 20%. Selection of varieties that produce high juice volume with high sugar content and strategies to inhibit seed formation may result in improved juice quality.展开更多
[Objective] The paper was to study the effects of different levels of NaOH on fiber, crude protein and soluble sugar content of corn straw. [Method] Four treatments were designed in the test, namely control group, gro...[Objective] The paper was to study the effects of different levels of NaOH on fiber, crude protein and soluble sugar content of corn straw. [Method] Four treatments were designed in the test, namely control group, group 1, group 2 and group 3, which were added with 0, 2%, 3%and 4% NaOH according to the dry weight of corn straw, and the moisture of corn straw after treatment was 45%. After being treated at room tem-perature for 48 h, sensory evaluation and nutrient composition analysis were performed for each group, and the contents of neutral detergent fiber(NDF), acid detergent fiber(ADF), crude protein(CP) and soluble sugar(SS) were measured. [Result] With the increasing addition level of NaOH,the content of NDF and ADF decreased gradually, and significant differences were observed between group 3(adding 4% NaOH) and control group(P<0.05). There was no significant difference in CP content among treatment groups(P>0.05); the SS content gradually increased, and there were significant differences between treatment groups and control group(P<0.05). [Conclusion] 4% NaOH reduced the fiber content and increased the soluble sugar content of corn straw.展开更多
The transformation of aldose to ketose or common sugars into rare saccharides,including rare ketoses and aldoses,is of great value and interest to the food industry and for saccharidic biomass utilization,medicine,and...The transformation of aldose to ketose or common sugars into rare saccharides,including rare ketoses and aldoses,is of great value and interest to the food industry and for saccharidic biomass utilization,medicine,and the synthesis of drugs.Nowadays,high-fructose corn syrup(HFCS)is industrially produced in more than 10 million tons annually using immobilized glucose isomerase.Some low-calorie saccharides such as tagatose and psicose,which are becoming popular sweeteners,have also been produced on a pilot scale in order to replace sucrose and HFCS.However,current catalysts and catalytic processes are still difficult to utilize in biomass conversion and also have strong substrate dependence in producing high-value,rare sugars.Considering the specific reaction properties of saccharides and catalysts,since the pioneering discovery by Fischer,various catalysts and catalytic systems have been discovered or developed in attempts to extend the reaction pathways,improve the reaction efficiency,and to potentially produce commercial products.In this review,we trace the history of sugar isomerization/epimerization reactions and summarize the important breakthroughs for each reaction as well as the difficulties that remain unresolved to date.展开更多
Sugar cane juice is a popular refreshing drink in most part of Bangladesh. It has great taste and health benefits;also it is available most of the public places at reasonable prices which consumed by road side custome...Sugar cane juice is a popular refreshing drink in most part of Bangladesh. It has great taste and health benefits;also it is available most of the public places at reasonable prices which consumed by road side customers including general public, shopping personals, tourists, students. In our country, street vendors crushing sugarcane between roller crusher and sold without any heat treatment or preservative, also served with or without added ice and lemon juice. The study aimed to identify and compare the physico-chemical and microbial quality of sugarcane juice. The chemical qualities of juices including moisture, P<sup>H</sup>, ash, total soluble solid, total sugar, reducing sugar and titrable acidity were found slightly different in Mymensingh, Gazipur and Narayangonj areas. The highest and lowest value of moisture, ash, P<sup>H</sup>, total soluble solids, total sugar, reducing sugar and titrable acidity were found in 84.33% - 79.26% (Gazipur-Mymensingh), 0.57% - 0.04% (Mymensingh-Gazipur), 5.9 - 2.9 (Gazipur-Narayangonj), 17.48% - 4.98% (Mymensingh-Narayangonj), 21.9% - 6.56% (Mymensingh-Gazipur), 3.7 - 2.1 (Gazipur-Mymensingh) and 0.523% - 0.007% (Narayangonj-Gazipur) respectively. For microbiological analysis, the total viable count of sugar cane juice in Mymensingh, Gazipur and Narayangonj were ranged from 0.6 × 10<sup>5</sup> - 43.6 × 10<sup>5</sup> cfu/ml, 4.6 × 10<sup>5</sup> - 21.6 × 10<sup>5</sup> cfu/ml and 3.6 × 10<sup>5</sup> - 36.6 × 10<sup>5</sup> cfu/ml respectively, where the permitted value is 1.0 × 10<sup>4</sup> cfu/ml, whereas the total coliform count was ranged from 0.4 × 10<sup>5</sup> - 6.4 × 10<sup>5</sup> cfu/ml, 0.6 × 10<sup>5</sup> - 8.4 × 10<sup>5</sup> cfu/ml and 0.00 - 8.4 × 10<sup>5</sup> cfu/ml, where the permitted value is 100 cfu/ml and total fungal count was ranged from 5.5 × 10<sup>5</sup> - 56.5 × 10<sup>5</sup> cfu/ml, 21.5 × 10<sup>5</sup> - 54.5 × 10<sup>5</sup> cfu/ml and 32.5 × 10<sup>5</sup> - 68.5 × 10<sup>5</sup> cfu/ml, where the permitted value is 1000 cfu/ml. According to the Gulf standard, the microbiological parameters of all the collected sugar cane juice were out of the permitted standards, so that a serious health outbreak can be caused anytime.展开更多
基金supported by the National Natural Science Foundation of China(Projects 32030100,32102362),Natural Science Foundation of Beijing(6222004)National Key Research and Development Program(2018YFD1000200)+1 种基金Science and Technology Innovation Support Program(BUA-HHXD2022005)Research and Innovation Ability Improvement Program for Young Teachers of Beijing University of Agriculture.
文摘Vacuolar Phosphate Transporter1(VPT1)-mediated phosphate uptake in the vacuoles is essential to plant development and fruit ripening.Interestingly,here we find that the VPT1 may transport sugar in response to soluble sugar status of fruits.The VvVPT1 protein isolated from grape(Vitis vinifera)berrieswas tonoplast-localized and contains SPX(Syg1/Pho81/XPR1)and MFS(major facilitator superfamily)domains.Its mRNA expression was significantly increased during fruit ripening and induced by sucrose.Functional analyses based on transient transgenic systems in grape berry showed that VvVPT1 positively regulated berry ripening and significantly affected hexose contents,fruit firmness,and ripening-related gene expression.The VPT1 proteins(Grape VvVPT1,strawberry FaVPT1,and Arabidopsis AtVPT1)all showed low affinity for phosphate verified in yeast system,while they appear different in sugar transport capacity,consistent with fruit sugar status.Thus,our findings reveal a role for VPT1 in fruit ripening,associated to its SPX and MFS domains in direct transport of soluble sugar available into the vacuole,and open potential avenues for genetic improvement in fleshy fruit.
基金supported by the National Natural Science Foundation of China(32160695,32160718)the Natural Science Foundation of Guizhou Province[ZK(2022)301],Guizhou Normal University QSXM[2022]19,the Project of Basic Research Plan in Yunnan(202101BC070003+1 种基金202302AE090005)the Natural Science Research Project of Guizhou Provincial Department of Education[KY(2022)170],the Guizhou Scholarship for Overseas Students[(2019)12], the Innovation Program of Postgraduate Education of Guizhou Province[Qian Jiao He YJSCXJH(2020)098].
文摘Vaccinium duclouxii,endemic to southwestern China,is a berry-producing shrub or small tree belonging to the Ericaceae family,with high nutritive,medicinal,and ornamental value,abundant germplasm resources,and good edible properties.In addition,V.duclouxii exhibits strong tolerance to adverse environmental conditions,making it a promising candidate for research and offering wide-ranging possibilities for utilization.However,the lack of V.duclouxii genome sequence has hampered its development and utilization.Here,a high-quality telomere-to-telomere genome sequence of V.duclouxii was de novo assembled and annotated.All of 12 chromosomes were assembled into gap-free single contigs,providing the highest integrity and quality assembly reported so far for blueberry.The V.duclouxii genome is 573.67 Mb,which encodes 41953 protein-coding genes.Combining transcriptomics and metabolomics analyses,we have uncovered the molecular mechanisms involved in sugar and acid accumulation and anthocyanin biosynthesis in V.duclouxii.This provides essentialmolecular information for further research on the quality of V.duclouxii.Moreover,the high-quality telomere-to-telomere assembly of the V.duclouxii genome will provide insights into the genomic evolution of Vaccinium and support advancements in blueberry genetics and molecular breeding.
基金financially supported by the National Natural Science Foundation of China(31701449,31971968,31971899,and 31501332)the Natural Science Foundation of Heilongjiang,China(QC2017013)+7 种基金the National Key R&D Program of China(2016YFD0100500,2016YFD0100300 and 2016YFD0100201-21)the Special Financial Aid to PostDoctor Research Fellow in Heilongjiang,China(LBHTZ1714)the International Postdoctoral Exchange Fellowship Program of China Postdoctoral Council(20180004)the China Post Doctoral Project,China(2015M581419)the Post-Doctoral Project of Northeast Agricultural University,China(NEAUBH-19002)the Heilongjiang Funds for Distinguished Young Scientists,China(JC2016004 and JC2017006)the Dongnongxuezhe Project,China(to Chen Qingshan)the the Backbone of Young Talent Scholar Project(to Qi Zhaoming,18XG01)of Northeast Agricultural University,China。
文摘Soluble sugar content in seeds is an important quality trait of soybean. In this study, 57 quantitative trait loci(QTLs) related to soluble sugar contents in soybean seeds were collected from databases and published papers. After meta-overview-collinearity integrated analysis to refine QTL intervals, eight consensus QTLs were identified. To further verify the consensus QTLs, a population of chromosome segment substitution lines(CSSLs) was analyzed. Two lines containing fragments covering the regions of consensus QTLs and the recurrent parent were selected: one line showed high soluble sugar contents associated with a consensus QTL fragment, and the other line showed low soluble sugar contents. Transcriptome sequencing was conducted for these two lines at the early, middle, and late stages of seed development, which identified 158, 109 and 329 differentially expressed genes, respectively. Based on the analyses of re-sequencing data of the CSSLs and the consensus QTL region, three candidate genes(Glyma.19 G146800, Glyma.19 G122500, and Glyma.19 G128500) were identified in the genetic fragments introduced from wild soybean. Sequence comparisons between the two CSSL parents SN14 and ZYD00006 revealed a single nucleotide polymorphism(SNP) mutation in the coding sequence of Glyma.19 G122500, causing a nonsynonymous mutation in the amino acid sequence that affected the predicted protein structure. A Kompetitive allele-specific PCR(KASP) marker was developed based on this SNP and used to evaluate the CSSLs. These results lay the foundation for further research to identify genes related to soluble sugar contents in soybean seeds and for future soybean breeding.
基金supported by the National Key Research and Development Program of China(2021YFD1201605)the National Natural Science Foundation of China(3216114303 and 32001574)Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences。
文摘Soluble sugar is a key quality trait of soybean seeds.We developed rapid and economic extraction and quantification methods for seed soluble sugars using an ultra-high performance liquid chromatography system with a refractive index detector.We evaluated the soluble sugar compositions of 1164 soybean accessions collected from diverse ecoregions and grown in multiple locations and years.Total soluble sugar(TSS)content was influenced by accession type,year of cultivation,and ecoregion.The mean contents of fructose,glucose,sucrose,raffinose,stachyose and TSS were 3.31,5.21,55.60,6.60,35.47,and 106.19 mg g^(-1),respectively.The highest mean TSS content(108.71 mg g^(-1)) was observed in accessions from Northern Region of China.Cultivars contained higher contents of sucrose,raffinose,and TSS,whereas landraces had a higher content of stachyose.Fourteen accessions with mean TSS contents>130 mg g^(-1) were identified as elite soybean resources.TSS was correlated with sucrose,raffinose,stachyose,protein,oil and total tocopherol.The main soluble sugar components were correlated with latitude and longitude,indicating that the geographical origin of the accessions affected their seed soluble sugar compositions.The developed methods and elite identified accessions can be used in the food and feed industry and in soybean breeding programs aimed at improving soybean seed nutrition.
基金The study was supported by the National Natural Science Foundation of China （30170640）.
文摘Liaoyuanduoli tomato was planted in pots in a solar greenhouse. The first leaf below the second cluster was labeled by14CO2 with a dose of 0.555 MBq 30 days after the second cluster anthesis. Mesophyll, midrib, petiole vascular of the fedleaf, internode vascular below the fed leaf (adjacent to the fed leaf), peduncle vascular of the second cluster, sepal, fruitpedicle, pulp, pectinic, and dissepiment were taken 2, 4, 8, 12, 20, 24, 48, and 72 h, respectively, after 14CO2 feed todeterminate the composition and specific activity of carbohydrate at different sites and different time after the labeling.The results indicated that almost all of the photosynthates resulting from 1 h of photosynthesis could be transported outof the leaf within 72 h and more than 85% of them were exported within 24 h. The greatest amount of sucrose transportoccurred in the internode pedicel vascular bundle 8 h after the start of photosynthesis. Assimilates entering the fruitthrough the internode and pedicel vascular bundles were detectable but in small quantities 2 h after photosynthesis. Onlythe radio activity of sucrose but not that of glucose and fructose was detected, suggesting that some of the sucrose in thefruit came directly through the phloem at an early stage of fruit development, not through the synthesis of glucose andfructose. Fruit vascular may be the part where sucrose first metabolized after it entered the fruit.
基金supported in part by funds from International Foundation for Science(C/5262-1)
文摘The content of soluble sugar in cowpea(Vigna unguiculata L. Walp.) seeds is important for their acceptability to consumers. The objective of this study was to evaluate the genetic control of soluble sugar content in cowpea using diallel analysis. For this purpose,half-diallel crosses were made among seven diverse parents. The amount of total soluble sugar was estimated by the phenol sulfuric acid reagent method and reducing sugar were measured by the DNS(3,5-dinitrosalicylic acid) reagent method. The total soluble sugar ranged from 11.12(VYA) to 40.79 mg g^(-1)(IT93 K-693-2) with a mean of 22.31 mg g^(-1).Reducing soluble sugar showed a mean value of 9.11 mg g^(-1) and ranged from 13.61(TVx3236) to 4.98 mg g^(-1)(Lori niebe). Diallel analysis showed that both additive and non-additive gene effects were significant in the genetic control of these traits. However,dominance variance was greater than additive variance for soluble sugar. The traits were controlled mainly by an overdominance model, suggesting the advantage of delaying selection to later generations. Soluble sugar content was found to be highly heritable, with broad-sense heritability ranging from 0.987 to 0.976. Narrow-sense heritability ranged from0.154 to 0.422 and revealed the lower importance of additive variance. Parents and F_1 hybrids differed significantly on the basis of their general and specific combining abilities,respectively. Recessive alleles had positive effects on total soluble sugar and reducing soluble sugar. These results could help cowpea breeders improve the acceptability of cowpea seeds and cowpea-based foods by appropriate selection.
文摘High temperature stress on different varieties of lettuce seedlings in MDA, proline and soluble sugar content were studied. The results were as follows : With the stress temperature, varieties of leaf lettuce seedlings in the MDA, proline and soluble sugar content gradually increased;Different varieties had the most significant difference in 38/33℃(d/n). The increase of heat-resistant varieties on proline and soluble sugar content was quick and high, while its increase in MDA content was slow and small.
基金supported by the National Natural Science Foundation of China(31060174,30660084)the Natural Science Foundation of Inner Mongolia,China(2010ZD07,200607010301)+1 种基金the Technology System of Agricultural Industry of China(CARS-08-B-5)the Item of Science Innovation Team of Inner Mongolia Agricultural University(NDTD2010-8)
文摘Alkali stress can cause severe crop damage and reduce production. However, physiological processes involved in alkali stress in oat seedlings are not well understood. In this study, physiological responses and yield of oat to alkali stress were studied using the alkali-tolerant oat genotype Vao-9 and the alkali-sensitive oat genotype Baiyan 5. The results were: (i) low concentrations of alkali stress (25 and 50 mmol L-1) significantly reduced the yield and grain weight while increased the oat grain number per spike. A negative correlation between yield and malondialdehyde (MDA) content at the jointing and grain filling stages and positive correlations between yield on one hand and superoxide dismutase (SOD), and peroxidase (POD) activities on the other at the jointing stage were observed. There was a positive correlation between MDA and soluble sugar at the grain filling stage; (ii) soluble sugar content was increased at the jointing and grain filling stages and decreased at the heading stage by alkali stress; (iii) alkali stress increased the SOD activity during the heading and grain filling stages, and increased the POD activity at the heading stage. As compared to the control, the increase of MDA contents in alkali-treated oat was observed, during the jointing, heading and grain filling stages; (iv) under alkali stress, the oat genotype Vao-9 showed higher antioxidant enzyme activity and lower soluble sugar contents during the heading stage, and lower MDA contents than those in the oat genotype Baiyan 5 under alkali stress. The result suggested that the high ROS scavenging capacity and soluble sugar levels might play roles in oat response to alkali stress.
文摘Cowpea (Vigna unguiculata (L.) Walp) is a legume crop grown worldwide to provide protein, starch, soluble sugar, amino acids, fatty acids, minerals, and vitamins for human consumption and animal feed. Soluble sugar is an important physiological trait in cowpea seeds. It not only plays an important role in storability and abiotic stress tolerance in seeds, but also provides energy to the human body and improves cooking quality by giving a desirable taste and mouth feel. Therefore, this research aimed to evaluate variation of cowpea seed soluble sugar content, and assess the soluble sugar content of cowpea varieties with different seed coat colors for cowpea consumers. A total of 113 cowpea genotypes were used, including 89 USDA GRIN germplasm accessions and 24 advanced breeding lines developed from University of Arkansas, AR, USA. Seed soluble sugar content was measured using a Spectrophotometer by phenol-sulphuric acid method. The results showed that the seed soluble sugar contents among 113 cowpea genotypes exhibited a wide range varying from 32.6 mg/g to 86.1 mg/g with an average of 54.5 mg/g. The five highest soluble sugar contents in seeds were found in the Arkansas cultivar Empire (86.1 mg/g), USDA germplasm accession PI583202 (84.5 mg/g), Arkansas advanced breeding line 09-655 (82.1 mg/g), USDA accession PI601085 (81.6 mg/g), and Arkansas advanced breeding line 09-529 (80.9 mg/g), and they can be used in cowpea breeding programs to develop new cowpea cultivars with higher seed soluble sugar content. It was also observed that the soluble sugar contents in Arkansas commercial cultivars and advanced breeding lines were higher than those in USDA germplasm and the seeds with colorful coat may have soluble sugar levels desirable by consumers.
基金supported by the National Natural Science Foundation of China (30871473)the project from the China National Rice Research Institute (2009RG0035)+1 种基金the projects from Local Government of Zhejiang Province, China (2008C22073, 2009C32048, and SN200806)the Zhejiang Provincial Natural Science Foundation, China (Y3100060 and Y3100090)
文摘Two rice maintaining lines with different drought tolerances, viz., Jin 23B (tolerant) and Zhenshan 97B (sensitive), were used to study the oxidative stress and soluble sugar in rice anthers and pollen viability under drought stress during flowering stage. Higher antioxidant enzyme activities and lower malonaldehyde (MDA) content in rice anthers were observed in Jin 23B than in Zhenshan 97B under drought stress. Further, a great increase in the content of soluble sugar in rice anthers of Jin 23B was observed across the whole drought exposure, while Zhenshan 97B showed significant decrease in soluble sugar during 9-12 d after drought stress (DADS). Accordingly, a marked decline of pollen fertility and activity, pollen numbers in an anther and pollen numbers on a stigma was observed in Zhenshan 97B, whereas little difference was found in Jin 23B. Thus, we suggest that pollen abortion caused by drought stress may be related with the reciprocity between oxidative stress and soluble sugar content in rice anthers.
基金funded by Shanghai Agriculture Applied Technology Development Programs,China(Grant No.G2014070202 and No.G2019-02-08-00-08-F01108).
文摘Sugar from plant photosynthesis is a basic requirement for life activities.Sugar transporters are the proteins that mediate sugar allocation among or within source/sink organs.The transporters of the major facilitator superfamily(MFS)targeting carbohydrates represent the largest family of sugar transporters in many plants.Strawberry(Fragaria×ananassa Duchesne)is an important crop appreciated worldwide for its unique fruit flavor.The involvement of MFS sugar transporters(STs)in cultivated strawberry fruit sugar accumulation is largely unknown.In this work,we characterized the genetic variation associated with fruit soluble sugars in a collection including 154 varieties.Then,a total of 67 ST genes were identified in the v4.0 genome integrated with the v4.0.a2 protein database of F.vesca,the dominant subgenome provider for modern cultivated strawberry.Phylogenetic analysis updated the nomenclature of strawberry ST homoeologs.Both the chromosomal distribution and structural characteristics of the ST family were improved.Semi-RT-PCR analysis in nine tissues from cv.Benihoppe screened 34 highly expressed ST genes in fruits.In three varieties with dramatically differing fruit sugar levels,qPCR integrated with correlation analysis between ST transcript abundance and sugar content identified 13 sugar-correlated genes.The correlations were re-evaluated across 19 varieties,including major commercial cultivars grown in China.Finally,a model of the contribution of the sugar transporter system to subcellular sugar allocation in strawberry fruits was proposed.Our work highlights the involvement of STs in controlling strawberry fruit soluble sugars and provides candidates for the future functional study of STs in strawberry development and responses and a new approach for strawberry genetic engineering and molecular breeding.
基金Supported by Commonweal Specialized Research Fund of China Agriculture(Grant No.201003029)Collaborative Innovation Center of Grain and Oil crops in South China
文摘There are different levels of resistance in Chinese cabbage varieties against clubroot.The content of catalase(CAT),peroxidase(POD),phenylalanin ammonia-lyase(PAL),Super Oxide Dismutase(SOD)and soluble sugar activities of 3cultivars with different clubroot-resistant levelswere detected after inoculation in this study.The results suggest that the changing rates of CAT,PAL and SOD contents of resistance cultivars after inoculation were higher than those of susceptible cultivars(Baigengbaiye>Zaobaicaitai>Huangjinxiaobaicai);the POD activities of resistance cultivars reached a peak on an earlier day than those of susceptible cultivars;the increasing rate of soluble sugar of susceptible cultivars was higher than that of the resistance cultivars,and ended with two peaks.Therefore,the four enzymes(CAT,POD,PAL and SOD)and soluble sugar may be used as physiological and biochemical reference indexes for the resistance identification to clubroot after inoculation with P.brassicae.
文摘Drought is a major production constraint for major fruits and vegetable crops in the tropics. This study was conducted to investigate the effect of limited water supply at three growth stages(vegetative, flowering and fruiting) on the accumulation of proline and soluble sugars in three pepper species. Seeds of the three pepper species, Capsicum chinense Jacq., C. annuum L. and C. frutescens L. were raised in a nursery and the seedlings were transplanted into seventy two plastic pots arranged in a randomized complete block design with three replicates, 25 days after planting. Four water treatments, 200 m L of water supplied twice daily(W1), once in every three days(W2), once in every five days(W3), and zero water supplied throughout growing period(W0) were imposed at three vegetative, flowering, and fruiting growth stages. Data were collected on relative water content, free proline and total soluble sugar. Data collected were subjected to analysis of variance and means were separated using Duncan's multiple range test. Results show that the concentration of proline and soluble sugar in leaves of the three pepper species were found to be remarkable at the different stages of growth in the stressed plants.
基金supported by the National Natural Science Foundation of China(31772326)the National Key Research and Development Program of China(2017YFD0101802).
文摘Orphan genes(OGs),which are genes unique to a specific taxon,play a vital role in primary metabolism.However,little is known about the functional significance of Brassica rapa OGs(BrOGs)that were identified in our previous study.To study their biological functions,we developed a BrOG overexpression(BrOGOE)mutant library of 43 genes in Arabidopsis thaliana and assessed the phenotypic variation of the plants.We found that 19 of the 43 BrOGOE mutants displayed a mutant phenotype and 42 showed a variable soluble sugar content.One mutant,BrOG1OE,with significantly elevated fructose,glucose,and total sugar contents but a reduced sucrose content,was selected for indepth analysis.BrOG1OE showed reduced expression and activity of the Arabidopsis sucrose synthase gene(AtSUS);however,the activity of invertase was unchanged.In contrast,silencing of two copies of BrOG1 in B.rapa,BraA08002322(BrOG1A)and BraSca000221(BrOG1B),by the use of an efficient CRISPR/Cas9 system of Chinese cabbage(B.rapa ssp.campestris)resulted in decreased fructose,glucose,and total soluble sugar contents because of the upregulation of BrSUS1b,BrSUS3,and,specifically,the BrSUS5 gene in the edited BrOG1 transgenic line.In addition,we observed increased sucrose content and SUS activity in the BrOG1 mutants,with the activity of invertase remaining unchanged.Thus,BrOG1 probably affected soluble sugar metabolism in a SUS-dependent manner.This is the first report investigating the function of BrOGs with respect to soluble sugar metabolism and reinforced the idea that OGs are a valuable resource for nutrient metabolism.
文摘Alternative and renewable bio-based energy sources are gaining prominence worldwide. Sweet sorghum is currently being evaluated throughout the world because its stem juices are rich in sugars that can be directly fermented to ethanol. In this two-year study, sweet sorghum varieties;Dale, Theis, Topper 76-6, and M81E (Obtained from Mississippi State University Experiment Station, MS) and CHR-SW8 (Obtained from Chromatin Inc., IL) were used. Nitrogen (N) fertilizer rates of 0, 40, 80 or 120 kg·N·ha-1 were applied to experimental units. The experiment was a randomized complete block design with treatments in a split-split plot arrangement with three replications. Nitrogen rate was the main plot, cultivar as sub-plot, and panicle removal as sub-plot. Results showed that N application increased fresh stem yield, juice volume, but had minimal effect on juice soluble sugar concentration. Compared to controls, application of ≥40 kg·N·ha-1 increased fresh yield and juice by >60% and 10%, respectively. There were also variety differences in harvested fresh biomass, juice volume and oBrix, and soluble sugar content. Dale and Theis consistently showed lower sucrose compared to other varieties over the two years. Panicle removal during early reproductive phase increased oBrix, sucrose and total sugar content in all varieties. Across the two years of study, panicle removal increased oBrix by more than 10%, sucrose and total sugar increased by more than 20%. Selection of varieties that produce high juice volume with high sugar content and strategies to inhibit seed formation may result in improved juice quality.
基金Supported by PhD Start-up Fund of Inner Mongolia University for Nationalities(BS291)
文摘[Objective] The paper was to study the effects of different levels of NaOH on fiber, crude protein and soluble sugar content of corn straw. [Method] Four treatments were designed in the test, namely control group, group 1, group 2 and group 3, which were added with 0, 2%, 3%and 4% NaOH according to the dry weight of corn straw, and the moisture of corn straw after treatment was 45%. After being treated at room tem-perature for 48 h, sensory evaluation and nutrient composition analysis were performed for each group, and the contents of neutral detergent fiber(NDF), acid detergent fiber(ADF), crude protein(CP) and soluble sugar(SS) were measured. [Result] With the increasing addition level of NaOH,the content of NDF and ADF decreased gradually, and significant differences were observed between group 3(adding 4% NaOH) and control group(P<0.05). There was no significant difference in CP content among treatment groups(P>0.05); the SS content gradually increased, and there were significant differences between treatment groups and control group(P<0.05). [Conclusion] 4% NaOH reduced the fiber content and increased the soluble sugar content of corn straw.
基金Financial support by Dual Initiative Project of Jiangsu Province and Changzhou University is gratefully acknowledgedSample analysis supported by Analysis and Testing Center,NERC Biomass of Changzhou University was also greatly acknowledged.
文摘The transformation of aldose to ketose or common sugars into rare saccharides,including rare ketoses and aldoses,is of great value and interest to the food industry and for saccharidic biomass utilization,medicine,and the synthesis of drugs.Nowadays,high-fructose corn syrup(HFCS)is industrially produced in more than 10 million tons annually using immobilized glucose isomerase.Some low-calorie saccharides such as tagatose and psicose,which are becoming popular sweeteners,have also been produced on a pilot scale in order to replace sucrose and HFCS.However,current catalysts and catalytic processes are still difficult to utilize in biomass conversion and also have strong substrate dependence in producing high-value,rare sugars.Considering the specific reaction properties of saccharides and catalysts,since the pioneering discovery by Fischer,various catalysts and catalytic systems have been discovered or developed in attempts to extend the reaction pathways,improve the reaction efficiency,and to potentially produce commercial products.In this review,we trace the history of sugar isomerization/epimerization reactions and summarize the important breakthroughs for each reaction as well as the difficulties that remain unresolved to date.
文摘Sugar cane juice is a popular refreshing drink in most part of Bangladesh. It has great taste and health benefits;also it is available most of the public places at reasonable prices which consumed by road side customers including general public, shopping personals, tourists, students. In our country, street vendors crushing sugarcane between roller crusher and sold without any heat treatment or preservative, also served with or without added ice and lemon juice. The study aimed to identify and compare the physico-chemical and microbial quality of sugarcane juice. The chemical qualities of juices including moisture, P<sup>H</sup>, ash, total soluble solid, total sugar, reducing sugar and titrable acidity were found slightly different in Mymensingh, Gazipur and Narayangonj areas. The highest and lowest value of moisture, ash, P<sup>H</sup>, total soluble solids, total sugar, reducing sugar and titrable acidity were found in 84.33% - 79.26% (Gazipur-Mymensingh), 0.57% - 0.04% (Mymensingh-Gazipur), 5.9 - 2.9 (Gazipur-Narayangonj), 17.48% - 4.98% (Mymensingh-Narayangonj), 21.9% - 6.56% (Mymensingh-Gazipur), 3.7 - 2.1 (Gazipur-Mymensingh) and 0.523% - 0.007% (Narayangonj-Gazipur) respectively. For microbiological analysis, the total viable count of sugar cane juice in Mymensingh, Gazipur and Narayangonj were ranged from 0.6 × 10<sup>5</sup> - 43.6 × 10<sup>5</sup> cfu/ml, 4.6 × 10<sup>5</sup> - 21.6 × 10<sup>5</sup> cfu/ml and 3.6 × 10<sup>5</sup> - 36.6 × 10<sup>5</sup> cfu/ml respectively, where the permitted value is 1.0 × 10<sup>4</sup> cfu/ml, whereas the total coliform count was ranged from 0.4 × 10<sup>5</sup> - 6.4 × 10<sup>5</sup> cfu/ml, 0.6 × 10<sup>5</sup> - 8.4 × 10<sup>5</sup> cfu/ml and 0.00 - 8.4 × 10<sup>5</sup> cfu/ml, where the permitted value is 100 cfu/ml and total fungal count was ranged from 5.5 × 10<sup>5</sup> - 56.5 × 10<sup>5</sup> cfu/ml, 21.5 × 10<sup>5</sup> - 54.5 × 10<sup>5</sup> cfu/ml and 32.5 × 10<sup>5</sup> - 68.5 × 10<sup>5</sup> cfu/ml, where the permitted value is 1000 cfu/ml. According to the Gulf standard, the microbiological parameters of all the collected sugar cane juice were out of the permitted standards, so that a serious health outbreak can be caused anytime.
