为了解我国葡萄酒产区酒酒球菌种质资源遗传多样性,对22株筛选自我国不同葡萄酒产区的乳酸细菌进行了种特异性聚合酶链式反应(species-specific polymerase chain reaction,PCR)分析、16S r RNA序列分析和扩增片段长度多态性分析(amplif...为了解我国葡萄酒产区酒酒球菌种质资源遗传多样性,对22株筛选自我国不同葡萄酒产区的乳酸细菌进行了种特异性聚合酶链式反应(species-specific polymerase chain reaction,PCR)分析、16S r RNA序列分析和扩增片段长度多态性分析(amplified fragment length polymorphism,AFLP)基因分型。种特异性PCR和16S r RNA序列分析表明22株分离株为酒酒球菌(Oenococcus oeni)。建立了O.oeni基于HindⅢ和MseⅠ为内切酶的AFLP分析体系,对16对引物组合进行了筛选,结果表明HT-MA、HT-MT、HT-MC、HG-MA、HG-MT、HC-MT为O.oeni AFLP分析的最佳引物组合,且实验重复性在98%以上。对O.oeni的AFLP分析结果显示:22株O.oeni分为3个簇群,且簇群间遗传相似性系数较小。所以,以HindⅢ和MseⅠ为内切酶的AFLP技术是研究O.oeni基因分型的有效方法,我国葡萄酒产区的O.oeni具有丰富的遗传多样性,O.oeni菌株间的遗传相似性系数不仅仅与其生态地理分布有关,可能还与其所处的微生态和其他因素有关。展开更多
我们利用特定位点扩增片段测序技术(specific-length amplified fragment sequencing,SLAF-seq)对籼稻窄叶青8(ZYQ8)和粳稻京系17(JX17)构建的双单倍体(doubled haploid,DH)群体进行了简化基因组测序。将DH群体测序数据与水稻参考基因...我们利用特定位点扩增片段测序技术(specific-length amplified fragment sequencing,SLAF-seq)对籼稻窄叶青8(ZYQ8)和粳稻京系17(JX17)构建的双单倍体(doubled haploid,DH)群体进行了简化基因组测序。将DH群体测序数据与水稻参考基因组进行比较分析,获得了70 997个在两个亲本中存在多态性的SLAF标签。通过进一步的筛选,我们利用8 940个高质量的SLAF标签构建了该群体的遗传图谱。图谱覆盖12个连锁群,总图距为2 294 71 c M,标记间的平均图距为0.26 c M。遗传图谱的连锁群与水稻物理图的相关系数统计显示,除了9号连锁群的相关系数为0.87外,其余的11个连锁群的相关系数均在0.95以上,表明该图谱的12个连锁群与水稻物理图有很好共线性关系。该高密度图谱的构建为我们进一步研究籼、粳差异基因提供了帮助。展开更多
The blood clam(Tegillarca granosa)is an economically important marine shellfish in the southeast coastal area of China.In T.granosa breeding practice,growth-related traits are most important phenotypic selection index...The blood clam(Tegillarca granosa)is an economically important marine shellfish in the southeast coastal area of China.In T.granosa breeding practice,growth-related traits are most important phenotypic selection index.In order to increase the breeding efficiency,we used a previously published genetic linkage map of T.granosa to map quantitative trait loci(QTL)for growth-related traits in a single full sibling F1 family.Eight growth-related traits including shell length(SL),shell height(SH),shell width(SW),shell weight(Swi),shell umbo width(SUW),as well as outer ligament length(OLL),meat weight(MW)and total weight(TW)of T.granosa were measured for QTL mapping.The estimated phenotypic correlations between any 2 of the 8 growth-related traits were significant(P<0.001).Twenty-nine QTL were identified for growth-related traits,including 13 QTL with the phenotypic variance of 4.20-15.63%in the female map and 16 QTL of 3.99%-14.58%in the male map.Thirteen amplification fragment length polymorphism(AFLP)markers were validated to closely link to seven QTL except the shell height.This is the first report of QTL mapping on growth-related traits in T.granosa.These QTL and their tightly linked markers present the initial step towards the goal of marker-assisted selection in T.granosa.展开更多
文摘为了解我国葡萄酒产区酒酒球菌种质资源遗传多样性,对22株筛选自我国不同葡萄酒产区的乳酸细菌进行了种特异性聚合酶链式反应(species-specific polymerase chain reaction,PCR)分析、16S r RNA序列分析和扩增片段长度多态性分析(amplified fragment length polymorphism,AFLP)基因分型。种特异性PCR和16S r RNA序列分析表明22株分离株为酒酒球菌(Oenococcus oeni)。建立了O.oeni基于HindⅢ和MseⅠ为内切酶的AFLP分析体系,对16对引物组合进行了筛选,结果表明HT-MA、HT-MT、HT-MC、HG-MA、HG-MT、HC-MT为O.oeni AFLP分析的最佳引物组合,且实验重复性在98%以上。对O.oeni的AFLP分析结果显示:22株O.oeni分为3个簇群,且簇群间遗传相似性系数较小。所以,以HindⅢ和MseⅠ为内切酶的AFLP技术是研究O.oeni基因分型的有效方法,我国葡萄酒产区的O.oeni具有丰富的遗传多样性,O.oeni菌株间的遗传相似性系数不仅仅与其生态地理分布有关,可能还与其所处的微生态和其他因素有关。
文摘我们利用特定位点扩增片段测序技术(specific-length amplified fragment sequencing,SLAF-seq)对籼稻窄叶青8(ZYQ8)和粳稻京系17(JX17)构建的双单倍体(doubled haploid,DH)群体进行了简化基因组测序。将DH群体测序数据与水稻参考基因组进行比较分析,获得了70 997个在两个亲本中存在多态性的SLAF标签。通过进一步的筛选,我们利用8 940个高质量的SLAF标签构建了该群体的遗传图谱。图谱覆盖12个连锁群,总图距为2 294 71 c M,标记间的平均图距为0.26 c M。遗传图谱的连锁群与水稻物理图的相关系数统计显示,除了9号连锁群的相关系数为0.87外,其余的11个连锁群的相关系数均在0.95以上,表明该图谱的12个连锁群与水稻物理图有很好共线性关系。该高密度图谱的构建为我们进一步研究籼、粳差异基因提供了帮助。
基金supported by the National Key R&D Program of China (2020YFD0900802)National Natural Science Foundation of China (Nos.31902358)+1 种基金the Natural Science Foundation of Shandong Province (ZR2020MC192)the“First class fishery discipline”programme in Shandong Province,China,programme in Shandong Province,China.
文摘The blood clam(Tegillarca granosa)is an economically important marine shellfish in the southeast coastal area of China.In T.granosa breeding practice,growth-related traits are most important phenotypic selection index.In order to increase the breeding efficiency,we used a previously published genetic linkage map of T.granosa to map quantitative trait loci(QTL)for growth-related traits in a single full sibling F1 family.Eight growth-related traits including shell length(SL),shell height(SH),shell width(SW),shell weight(Swi),shell umbo width(SUW),as well as outer ligament length(OLL),meat weight(MW)and total weight(TW)of T.granosa were measured for QTL mapping.The estimated phenotypic correlations between any 2 of the 8 growth-related traits were significant(P<0.001).Twenty-nine QTL were identified for growth-related traits,including 13 QTL with the phenotypic variance of 4.20-15.63%in the female map and 16 QTL of 3.99%-14.58%in the male map.Thirteen amplification fragment length polymorphism(AFLP)markers were validated to closely link to seven QTL except the shell height.This is the first report of QTL mapping on growth-related traits in T.granosa.These QTL and their tightly linked markers present the initial step towards the goal of marker-assisted selection in T.granosa.
