Commercial production of spinach(Spinacia oleracea L.)is centered in California and Arizona in the US,where downy mildew caused by Peronospora effusa is the most destructive disease.Nineteen typical races of P.effusa ...Commercial production of spinach(Spinacia oleracea L.)is centered in California and Arizona in the US,where downy mildew caused by Peronospora effusa is the most destructive disease.Nineteen typical races of P.effusa have been reported to infect spinach,with 16 identified after 1990.The regular appearance of new pathogen races breaks the resistance gene introgressed in spinach.We attempted to map and delineate the RPF2 locus at a finer resolution,identify linked single nucleotide polymorphism(SNP)markers,and report candidate downy mildew resistance(R)genes.Progeny populations segregating for RPF2 locus derived from resistant differential cultivar Lazio were infected using race 5 of P.effusa and were used to study for genetic transmission and mapping analysis in this study.Association analysis performed with low coverage whole genome resequencing-generated SNP markers mapped the RPF2 locus between 0.47 to 1.46 Mb of chromosome 3 with peak SNP(Chr3_1,221,009)showing a LOD value of 61.6 in the GLM model in TASSEL,which was within 1.08 Kb from Spo12821,a gene that encodes CC-NBS-LRR plant disease resistance protein.In addition,a combined analysis of progeny panels of Lazio and Whale segregating for RPF2 and RPF3 loci delineated the resistance section in chromosome 3 between 1.18–1.23 and 1.75–1.76 Mb.This study provides valuable information on the RPF2 resistance region in the spinach cultivar Lazio compared to RPF3 loci in the cultivar Whale.The RPF2 and RPF3 specific SNP markers,plus the resistant genes reported here,could add value to breeding efforts to develop downy mildew resistant cultivars in the future.展开更多
[Objectives]This study was conducted to promote the rational use of bioorganic fertilizers.[Methods]Stanley bioorganic fertilizer was selected to investigate the laws and characteristics of the effects of bioorganic f...[Objectives]This study was conducted to promote the rational use of bioorganic fertilizers.[Methods]Stanley bioorganic fertilizer was selected to investigate the laws and characteristics of the effects of bioorganic fertilizer on spinach growth with different application rates in a short term and found out the precise application rate.[Results]As the application rate of bioorganic fertilizer increasing,the emergence rate of spinach decreased.The total weights and leaf areas of spinach plants treated with different application rates of bioorganic fertilizers were all higher than those of the control check(CK).The total weights and leaf areas of spinach plants applied with bioorganic fertilizer at different rates were all higher than those of the CK.The total weight of spinach plants increased linearly with the application rate of bioorganic fertilizer increasing among treatments,while the leaf area fluctuated with the increase of bioorganic fertilizer concentration among various treatments.The average root volumes and average diameters of treatments applied with bioorganic fertilizer at different rates were all higher than those in the CK,but the average root length showed a different trend.The average root length was higher in the CK than in various bioorganic fertilizer treatments in the early growth period,but the differences of various treatments from the CK gradually decreased in the middle period,and in the later period,the root length of spinach treated with organic fertilizer gradually exceeded the CK.[Conclusions]This study provides a theoretical basis for the rational application of bioorganic fertilizers in spinach production.展开更多
To find a new way treating the wastewater from biogas reactors in a pig farm, vegetated floating bed was built for observing the water-purifying capability of water spinach (Ipomoea aquatic) planted on the water surfa...To find a new way treating the wastewater from biogas reactors in a pig farm, vegetated floating bed was built for observing the water-purifying capability of water spinach (Ipomoea aquatic) planted on the water surface. Experiments were carried out to record the growth and biomass accumulation of water spinach and its effect on purification of biogas wastewater. The results show that the water which mixed with biogas wastewater has been purified significantly by water spinach on the floating bed. During its growth season within four months, the overall length of water spinach reached 199 ± 35 cm, while its root length reached 63 ± 28.6 cm. The average weight of individual fresh plant is of 1285 ± 619.7 g. Meanwhile, the concentration of total nitrogen (TN) in water under the floating bed decreased from 8.9 ± 0.062 mg·L-1 to 0.5 ± 0.011 mg·L-1;the concentration of total phosphorus (TP) decreased from 4.4 ± 0.236 mg·L-1 to 0.92 ± 0.024 mg·L-1;the concentration of chemical oxygen demand (COD) decreased from 87.3 ± 6.68 mg·L-1 to 0.74 ± 0.46 mg·L-1. It suggests that the water spinach removed more than 90% of pollutants in terms of TN, TP, and COD from the water. Results show that the vegetated floating bed technique is a feasible way to dispose the biogas slurry.展开更多
Rising global CO<sub>2</sub> levels are a major factor that impacts not only the environment but also many plant functions including growth, productivity and nutritional quality. The study examined the imp...Rising global CO<sub>2</sub> levels are a major factor that impacts not only the environment but also many plant functions including growth, productivity and nutritional quality. The study examined the impact of elevated [CO<sub>2</sub>] on nutritional quality and growth characteristics of lettuce (Lactuca sativa) and spinach (Spinacia oleracea). Elevated [CO<sub>2</sub>] decreased the concentration of many important nutrients including nitrogen (protein), potassium and phosphorus in the edible parts of both lettuce and spinach. The nitrogen concentration in lettuce shoots was reduced by more than 30% at elevated [CO<sub>2</sub>] compared to the plants grown at ambient level of CO<sub>2</sub>. Similarly the concentration of a number of micronutrients including sulfur, zinc, copper and magnesium, was depressed in lettuce shoots. Although the total phenolic content and antioxidant capacity were higher in lettuce at elevated CO<sub>2</sub>, they were not affected in spinach. The photosynthetic activity was variable among the plant species while there was no increase in the carbon accumulation in these plants at elevated [CO<sub>2</sub>]. However, there was significant reduction in the leaf stomatal conductance in both lettuce and spinach in response to higher [CO<sub>2</sub>], which is likely affect both water loss from the leaves and their photosynthetic activity. The results indicate a broad adverse impact of rising [CO<sub>2</sub>] on the nutritional quality of commonly consumed leafy vegetables namely, lettuce and spinach.展开更多
To decrease fertilization amount of chemical fertilizer and improve the quality of vegetable crops,spinach was taken as the test material,and the impact of different fertilizer on spinach growth and yield was studied ...To decrease fertilization amount of chemical fertilizer and improve the quality of vegetable crops,spinach was taken as the test material,and the impact of different fertilizer on spinach growth and yield was studied via the manners of biological organic fertilizer and organic fertilizer + chemical fertilizer. Experimental results showed that in the formula of organic fertilizer + chemical fertilizer,chlorophyll and nitrogen contents in spinach leaves obviously increased; in the formula of only adding organic fertilizer,spinach leaf temperature,leaf width,root length,plant height and fresh weight were all better than those in the formula of organic fertilizer + chemical fertilizer,and better formulas were A_5,E_5,F_3 and I_5,in which spinach plant height in E_5 was 5. 63 times higher than G_5,root length in E_5 was 2. 67 times higher than G_5,and fresh weight in G_5 was 32. 6 times higher than G_5. By comprehensive analysis,the most suitable formula for spinach production was E_5,and the research could provide theoretic basis for fertilization amount of organic fertilizer required by spinach growth and development.展开更多
Lead is a toxic and naturally occurring substance with documented neurotoxin, toxic, and long-lasting adverse health effects globally. Lead exposure can cause impaired physical and mental development in children. Expo...Lead is a toxic and naturally occurring substance with documented neurotoxin, toxic, and long-lasting adverse health effects globally. Lead exposure can cause impaired physical and mental development in children. Exposure to high lead levels affects the intestinal tract, kidneys, joints and reproductive system in adults. This study evaluates the removal of 1500 PPM of lead from contaminated aqueous solution using Celite, Louisiana Red Clay, Charcoal, and supernatants from aqueous extracts of Mustard Green (Brassica juncea), and Spinach (Spinacea oleracea). After shaking triplicate reaction mixtures for each substrate for 22 hours at room temperature, lead removal by the five substrates were analyzed by EPA Method 6010, using Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES). Results suggest that the order of lead removal is Spinach (98%) > Charcoal (96%) > LA Red Clay (88%) > Mustard Green (87%) > Celite (4%). The study concludes that liquid substrates such as the supernatants from pureed spinach and mustard green can effectively remove lead from contaminated water.展开更多
Chloroplasts were isolated from spinach cultured in lead chloride-present, Ce3+-administered, cerium chloride-administered lead chloride-present Hoagland's media or that of Hoagland's media. The experimental s...Chloroplasts were isolated from spinach cultured in lead chloride-present, Ce3+-administered, cerium chloride-administered lead chloride-present Hoagland's media or that of Hoagland's media. The experimental study demonstrated the effects of cerium (Ce) on distribution of light energy and photochemical activities of spinach chloroplast grown in lead (Pb)-present media. It was observed that Pb2+ significantly inhibited photosynthesis in spinach, including light absorption, energy transfer from LHCⅡ to photosystem Ⅱ, excitation energy distribution from photosystem I to photosystem Ⅱ, and transformation from light energy to electron energy and oxygen evolution of chloroplasts, and decreased spinach growth. However, Ce3+ treatment to Pb2+-present chloroplasts could obviously improve light absorption and excitation energy distribution in both photosystems and increase activity of photochemical reaction and oxygen evolution of chloroplasts. The results suggested that Ce3+ under Pb2+ stress could maintain the stability of chloroplast membrane, and improve photosynthesis of spinach chloroplast, thus promote spinach growth.展开更多
Spinach(Spinacia oleracea)is a diploid(2n=2x=12),wind-pollinated and highly heterozygous crop.The plants are mostly dioecious,although some monoecious plants exist.Spinach is an economically important cool-season leaf...Spinach(Spinacia oleracea)is a diploid(2n=2x=12),wind-pollinated and highly heterozygous crop.The plants are mostly dioecious,although some monoecious plants exist.Spinach is an economically important cool-season leafy vegetable crop.Demand for spinach is increasing worldwide,particularly due to its high nutritional content.Spinach is a versatile crop eaten raw or cooked and used as salads or mixed with other cuisines.This review article provides an overview of origin and domestication,genetic diversity and population structure,genetic and genomic resources,major diseases threatening spinach production,breeding progress,and synthesizing how these resources can help in spinach improvement.The rapid development of genomic and sequence resources of spinach has increased biological and genetics research and laid the foundation for adopting molecular breeding.Downy mildew is the most serious disease affecting spinach and breeding programs focus on developing cultivars resistant to continually emerging new races of downy mildew pathogens.The use of genomic and molecular resources and approaches offers promises in population improvement and hybrid development to address biotic and abiotic stresses production challenges and provide improved breeding materials and strategies against the rapidly changing pathogen races and climatic conditions.展开更多
Spinach is one of the main vegetable species.The study of spinach germplasm resources is beneficial to discovering excellent genes and accelerating the breeding efficiency,thereby further promoting the development of ...Spinach is one of the main vegetable species.The study of spinach germplasm resources is beneficial to discovering excellent genes and accelerating the breeding efficiency,thereby further promoting the development of the spinach industry.In this study,the origin,propagation,collection,preservation,genetic diversity and breeding status of spinach were reviewed,and the problems and prospects of spinach germplasm resources were put forward.This paper provides reference for the research and breeding of spinach germplasm resources.展开更多
In the present scenario,Deep Learning(DL)is one of the most popular research algorithms to increase the accuracy of data analysis.Due to intra-class differences and inter-class variation,image classification is one of...In the present scenario,Deep Learning(DL)is one of the most popular research algorithms to increase the accuracy of data analysis.Due to intra-class differences and inter-class variation,image classification is one of the most difficult jobs in image processing.Plant or spinach recognition or classification is one of the deep learning applications through its leaf.Spinach is more critical for human skin,bone,and hair,etc.It provides vitamins,iron,minerals,and protein.It is beneficial for diet and is readily available in people’s surroundings.Many researchers have proposed various machine learning and deep learning algorithms to classify plant images more accurately in recent years.This paper presents a novel Convolutional Neural Network(CNN)to recognize spinach more accurately.The proposed CNN architecture classifies the spinach category,namely Amaranth leaves,Black nightshade,Curry leaves,and Drumstick leaves.The dataset contains 400 images with four classes,and each type has 100 images.The images were captured from the agricultural land located at Thirumanur,Salem district,Tamil Nadu.The proposed CNN achieves 97.5%classification accuracy.In addition,the performance of the proposed CNN is compared with Support Vector Machine(SVM),Random Forest,Visual Geometry Group 16(VGG16),Visual Geometry Group 19(VGG19)and Residual Network 50(ResNet50).The proposed provides superior performance than other models,namely SVM,Random Forest,VGG16,VGG19 and ResNet50.展开更多
Extraction is most commonly used sample preparation technique in quantitative determination of all-trans-lutein in spinach. In our study RP-HPLC was applied to identify the constituents of spinach extract and to quant...Extraction is most commonly used sample preparation technique in quantitative determination of all-trans-lutein in spinach. In our study RP-HPLC was applied to identify the constituents of spinach extract and to quantify all-trans-lutein content with the mobile phase consisted of acetone and water. Due to sensitivity of all-trans-lutein, we compared effects of different extraction conditions;air and nitrogen atmosphere and ethanol, ethanol with added 0.1% TBHQ and ethanol with added 0.1% BHT as solvents. Results for the all-trans-lutein content in the spinach samples and spinach samples with standard addition obtained in air and in nitrogen confirmed the induced degradation of all-trans-lutein in oxygen from the air. Ethanol containing synthetic antioxidant TBHQ under nitrogen atmosphere gave the highest yield of extraction and recovery of all-trans-lutein from spinach.展开更多
Previous studies showed that rabbit muscle phosphofructokinase-1 (PFK-1) activity losses due to dilution, due to inhibition by ascorbate, and due to some lithium salts were prevented by rabbit muscle aldolase. Chicken...Previous studies showed that rabbit muscle phosphofructokinase-1 (PFK-1) activity losses due to dilution, due to inhibition by ascorbate, and due to some lithium salts were prevented by rabbit muscle aldolase. Chicken PFK-1 and fish PFK-1 interacted with ascorbate and were inhibited, consistent with a previously proposed function that ascorbate facilitates glycogen in resting muscle by inhibiting glycolysis. This report shows that a plant enzyme, spinach aldolase, has the same ability to prevent rabbit muscle PFK-1 activity loses as rabbit muscle aldolase and in some instances it was a better protector from activity losses than rabbit aldolase. Spinach aldolase also protected chicken and fish PFK-1s from inhibitions by ascorbate and from activity losses due to dilution. Prevention of losses PFK-1 activities from animal species by a plant protein, spinach aldolase, suggests an evolutionary conservative relationship between PFK-1s and aldolases.展开更多
Minor alleles(MA)have been associated with disease incidence in human studies,enabling the identification of diagnostic risk factors for various diseases.However,allelic mapping has rarely been performed in plant syst...Minor alleles(MA)have been associated with disease incidence in human studies,enabling the identification of diagnostic risk factors for various diseases.However,allelic mapping has rarely been performed in plant systems.The goal of this study was to determine whether a difference in MA prevalence is a strong enough risk factor to indicate a likely significant difference in disease resistance against white rust(WR;Albugo occidentalis)in spinach(Spinacia oleracea).We used WR disease severity ratings(WR-DSRs)in a diversity panel of 267 spinach accessions to define resistant-and susceptibility-associated groups within the distribution scores and then tested the single-nucleotide polymorphism(SNP)variants to interrogate the MA prevalence in the most susceptible(MS)vs.most resistant(MR)individuals using permutation-based allelic association tests.A total of 448 minor alleles associated with WR severity were identified in the comparison between the 25%MS and the 25%MR accessions,while the MA were generally similar between the two halves of the interquartile range.The minor alleles in the MS group were distributed across all six chromosomes and made up~71%of the markers that were also strongly associated with WR in parallel performed genome-wide association study.These results indicate that susceptibility may be highly determined by the disproportionate overrepresentation of minor alleles,which could be used to select for resistant plants.Furthermore,by focusing on the distribution tails,allelic mapping could be used to identify plant markers associated with quantitative traits on the most informative segments of the phenotypic distribution.展开更多
This pot-based study investigated the influence of poultry manure and 1:1 mixture of poultry manure+biochar(produced from farmyard manure[FYM]or wood),on the biomass production and concentration of heavy metals in lea...This pot-based study investigated the influence of poultry manure and 1:1 mixture of poultry manure+biochar(produced from farmyard manure[FYM]or wood),on the biomass production and concentration of heavy metals in leaves of lettuce and spinach.The concentration of mineral nitrogen(N)and soluble inorganic phosphorus(P)of soils cultivated with these vegetables was also investigated.The application of poultry manure or FYM biochar in soil as 10%(equivalent to 60 t ha^(-1),an estimated 1726.8 kg ha^(-1)N in poultry manure and 1353.9 kg ha^(-1)N in FYM)and 15%amendment(equivalent to 90 t ha^(-1),an estimated 2590.2 kg ha^(-1)N in poultry manure and 2030.8 kg ha^(-1)N in FYM)significantly decreased biomass production of lettuce as compared to control(no fertilizer added)treatment.However,mixture of poultry manure with wood-derived biochar at both application rates(i.e.,10%and 15%)and with FYM biochar at lower application rate(i.e.,10%)caused 2-3-fold increase in aboveground plant biomass and 2-14-fold increase in root biomass(p<0.05).Furthermore,as compared to control treatment,a significant~2-3-fold increase in aboveground plant biomass was also observed in response to mixture of poultry manure with wood-derived and FYM derived biochars at 10%amendment rates.As compared to control treatment,concentration of mineral N and soluble inorganic P were higher in soils of all other treatments.In spinach,amendment of poultry manure or its co-amendment with biochar of FYM significantly increased aboveground plant biomass at 7%(equivalent to 42 t ha^(-1))as compared to 3%and 5%amendment rates(equivalent to 18 and 30 t ha^(-1)respectively).The concentration of soil mineral N and soil soluble mineral P was not different between treatments.In lettuce,wood-derived biochar did not reduce concentration of heavy metals(i.e.,manganese(Mn),copper(Cu),iron,(Fe),cadmium(Cd),lead(Pb),nickel(Ni)and cobalt(Co)than FYM-derived biochar while in spinach,as compared to poultry manure,co-amendment of poultry manure with wood-derived biochar reduced concentration of heavy metals,indicating differential responses of crops to organic amendments.展开更多
The effects of antimicrobial edible films containing carvacrol and cinnamaldehyde on organic baby spinach were determined via sensory analysis and changes in physical properties. Edible films made from pulp of hibiscu...The effects of antimicrobial edible films containing carvacrol and cinnamaldehyde on organic baby spinach were determined via sensory analysis and changes in physical properties. Edible films made from pulp of hibiscus, apple, or carrot containing carvacrol or cinnamaldehyde at 0.5%, 1.5%, or 3% concentrations were added to organic baby spinach in plastic bags. These bags were stored at 4<span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#ffffff;"="">°</span>C for 20-24h before performing sensory evaluation and measuring changes in physical properties. A randomized block design with an affective test was used. Preference liking was evaluated based on a 9-point hedonic scale for aroma, color, freshness, mouthfeel, flavor, and overall acceptability. Additionally, panelists quantified each sample using a 5-point hedonic scale for pungency, browning, bitterness, off-odor, and sourness. The color and texture of spinach samples were measured. Edible films containing cinnamaldehyde had the highest preference liking based on aroma, color, freshness, mouthfeel, flavor, and overall acceptability than those containing carvacrol and were the most likely to be purchased by panelists;therefore, cinnamaldehyde can potentially be used as an alternative sanitization option. There were no significant (p ≤ 0.05) changes in firmness or color values between spinach treated with antimicrobial films and controls. The results provide the produce industry with options for incorporating antimicrobial films into salad bags without influencing the physical or sensory properties of baby spinach.展开更多
A study was conducted in the Bekaa valley of Lebanon aiming to produce spinach leaves with treated effluent from Joub Janine plant under two growing seasons. Two experiments were laid out in a randomized complete bloc...A study was conducted in the Bekaa valley of Lebanon aiming to produce spinach leaves with treated effluent from Joub Janine plant under two growing seasons. Two experiments were laid out in a randomized complete block design. The effect of water quality on the qualitative, quantitative aspects and microbiological contamination of leaves was assessed. The results showed that the treated wastewater from Joub Janine plant was of category III. The highest mean marketable yields was recorded for T7 (4727 g·m-2) followed by T6 (3533 g·m-2) that were drip irrigated with treated wastewater. The uptake of K, Mg, Na and Cl was significantly 49.09%, 30.20%, 96.79% and 33.20%, respectively, higher in the spring than in autumn. The nitrate levels in all treatments and seasons were below the maximum level in foodstuffs as provided by the European Commission regulations. For the lipophilic fraction, there was no significant difference among treatments and also among treatments and seasons interacting together and the highest hydrophilic fraction and total phenols levels recorded for the autumn rather than the summer season. In general, pathogenic bacteria was absent on spinach leaves for all treatments and growing seasons.展开更多
A pot experiment was carried out to investigate the yield of Indian spinach (Basella alba L.) and their uptake and availability of phosphorus from lime and phosphorus amended acidic soil. Four rates of lime (L) equiva...A pot experiment was carried out to investigate the yield of Indian spinach (Basella alba L.) and their uptake and availability of phosphorus from lime and phosphorus amended acidic soil. Four rates of lime (L) equivalent to 0, 500, 1000 and 2000 kg CaCO3 ha-1 and four rates of phosphorus (P) equivalent to 0, 50, 100, and 150 kg·P·ha-1 of TSP were applied in combinations as treatments. Dry matter yield, P concentrations in shoot and root and P uptake by Indian spinach were determined after harvesting 10 weeks old plant and soil samples were collected from each pot to measure available P by Olsen method. Both L and P and their combinations had significant (P 0.001) effects on shoot and root biomass, shoot and root P concentrations, P uptake by Indian spinach and P availability. Although lime and P increased biomass production, P concentrations of shoot and root, and its uptake by Indian spinach and available P, this effect was boosted by combining L with P applied. 1000 kg lime plus 100 kg P were adequate for plant growth. Available P was strongly and positively correlated (R2 = 0.909, P = 0.000) with P uptake by plant. Results of the present study indicated that lime and phosphorus could be used in combination to enhance plant growth.展开更多
A glasshouse pot experiment was conducted to investigate cadmium (Cd) tolerance on the growth of Chinese spinach (Spinacia Oleracea L.) grown in sandy loam soil and microbial respiration after application of lherzolit...A glasshouse pot experiment was conducted to investigate cadmium (Cd) tolerance on the growth of Chinese spinach (Spinacia Oleracea L.) grown in sandy loam soil and microbial respiration after application of lherzolite (0%, 2.5% and 5%) with added Cd (0, 2.5 and 5 mg·kg-1). Soil pH tended to increase with increasing application rate of lherzolite about 1.5 units after plant harvest. Plant growth and microbial respiration in one hand decreased with the rates of Cd application in soil but on the other hand lherzolite application in soil reduced Cd toxicity and enhanced plant growth and microbial respiration. Microbial respiration of soil showed significant positive relationship with shoot and root dry weight of spinach but negative relationship of Cd concentration in plant tissue. This result indicated that application of lherzolite detoxified Cd toxicity in plant resulted in an increase plant growth and microbial respiration in Cd contaminated soil.展开更多
Effects of conventional and optimized water and nitrogen managements on spinach (Spinacia olerocea L.)growth and soil mineral N (Nmi.) residues were compared in an open field experiment in which water balancemethod an...Effects of conventional and optimized water and nitrogen managements on spinach (Spinacia olerocea L.)growth and soil mineral N (Nmi.) residues were compared in an open field experiment in which water balancemethod and N recommendation with the KNS-system were included. It was shown that the conventionalwater treatment (seasonal irrigated amount: 175 mm) reduced spinach growth compared to the water balancetreatments (seasonal irrigated amount: 80 and 85 mm) at the same N supply level due to N loss throughleaching caused by excessive water supply. Although 309 kg N ha-1 was applied in the conventional Ntreatment, compared to 82 and 66 kg N ha-l in the optimum N treatments, no significant difference in cropyield was investigated between the N treatments with the same irrigation practice. N uptake in spinach andsoil residual Nmin contents were also significantly affected by the irrigation practices. The conventional watersupply not only decreased water use efficiency, but also展开更多
基金This research was supported by a USDA-SCRI grant(2017-51181-26830)a USDA-AMS SCMP grant(16SCCMAR0001)the USDA NIFA Hatch project(ARK0VG2018 and ARK02440)to AS.
文摘Commercial production of spinach(Spinacia oleracea L.)is centered in California and Arizona in the US,where downy mildew caused by Peronospora effusa is the most destructive disease.Nineteen typical races of P.effusa have been reported to infect spinach,with 16 identified after 1990.The regular appearance of new pathogen races breaks the resistance gene introgressed in spinach.We attempted to map and delineate the RPF2 locus at a finer resolution,identify linked single nucleotide polymorphism(SNP)markers,and report candidate downy mildew resistance(R)genes.Progeny populations segregating for RPF2 locus derived from resistant differential cultivar Lazio were infected using race 5 of P.effusa and were used to study for genetic transmission and mapping analysis in this study.Association analysis performed with low coverage whole genome resequencing-generated SNP markers mapped the RPF2 locus between 0.47 to 1.46 Mb of chromosome 3 with peak SNP(Chr3_1,221,009)showing a LOD value of 61.6 in the GLM model in TASSEL,which was within 1.08 Kb from Spo12821,a gene that encodes CC-NBS-LRR plant disease resistance protein.In addition,a combined analysis of progeny panels of Lazio and Whale segregating for RPF2 and RPF3 loci delineated the resistance section in chromosome 3 between 1.18–1.23 and 1.75–1.76 Mb.This study provides valuable information on the RPF2 resistance region in the spinach cultivar Lazio compared to RPF3 loci in the cultivar Whale.The RPF2 and RPF3 specific SNP markers,plus the resistant genes reported here,could add value to breeding efforts to develop downy mildew resistant cultivars in the future.
基金Supported by Vegetable Innovation Team of Shandong Modern Agricultural Industry Technology System(SDAIT-05-18)。
文摘[Objectives]This study was conducted to promote the rational use of bioorganic fertilizers.[Methods]Stanley bioorganic fertilizer was selected to investigate the laws and characteristics of the effects of bioorganic fertilizer on spinach growth with different application rates in a short term and found out the precise application rate.[Results]As the application rate of bioorganic fertilizer increasing,the emergence rate of spinach decreased.The total weights and leaf areas of spinach plants treated with different application rates of bioorganic fertilizers were all higher than those of the control check(CK).The total weights and leaf areas of spinach plants applied with bioorganic fertilizer at different rates were all higher than those of the CK.The total weight of spinach plants increased linearly with the application rate of bioorganic fertilizer increasing among treatments,while the leaf area fluctuated with the increase of bioorganic fertilizer concentration among various treatments.The average root volumes and average diameters of treatments applied with bioorganic fertilizer at different rates were all higher than those in the CK,but the average root length showed a different trend.The average root length was higher in the CK than in various bioorganic fertilizer treatments in the early growth period,but the differences of various treatments from the CK gradually decreased in the middle period,and in the later period,the root length of spinach treated with organic fertilizer gradually exceeded the CK.[Conclusions]This study provides a theoretical basis for the rational application of bioorganic fertilizers in spinach production.
文摘To find a new way treating the wastewater from biogas reactors in a pig farm, vegetated floating bed was built for observing the water-purifying capability of water spinach (Ipomoea aquatic) planted on the water surface. Experiments were carried out to record the growth and biomass accumulation of water spinach and its effect on purification of biogas wastewater. The results show that the water which mixed with biogas wastewater has been purified significantly by water spinach on the floating bed. During its growth season within four months, the overall length of water spinach reached 199 ± 35 cm, while its root length reached 63 ± 28.6 cm. The average weight of individual fresh plant is of 1285 ± 619.7 g. Meanwhile, the concentration of total nitrogen (TN) in water under the floating bed decreased from 8.9 ± 0.062 mg·L-1 to 0.5 ± 0.011 mg·L-1;the concentration of total phosphorus (TP) decreased from 4.4 ± 0.236 mg·L-1 to 0.92 ± 0.024 mg·L-1;the concentration of chemical oxygen demand (COD) decreased from 87.3 ± 6.68 mg·L-1 to 0.74 ± 0.46 mg·L-1. It suggests that the water spinach removed more than 90% of pollutants in terms of TN, TP, and COD from the water. Results show that the vegetated floating bed technique is a feasible way to dispose the biogas slurry.
文摘Rising global CO<sub>2</sub> levels are a major factor that impacts not only the environment but also many plant functions including growth, productivity and nutritional quality. The study examined the impact of elevated [CO<sub>2</sub>] on nutritional quality and growth characteristics of lettuce (Lactuca sativa) and spinach (Spinacia oleracea). Elevated [CO<sub>2</sub>] decreased the concentration of many important nutrients including nitrogen (protein), potassium and phosphorus in the edible parts of both lettuce and spinach. The nitrogen concentration in lettuce shoots was reduced by more than 30% at elevated [CO<sub>2</sub>] compared to the plants grown at ambient level of CO<sub>2</sub>. Similarly the concentration of a number of micronutrients including sulfur, zinc, copper and magnesium, was depressed in lettuce shoots. Although the total phenolic content and antioxidant capacity were higher in lettuce at elevated CO<sub>2</sub>, they were not affected in spinach. The photosynthetic activity was variable among the plant species while there was no increase in the carbon accumulation in these plants at elevated [CO<sub>2</sub>]. However, there was significant reduction in the leaf stomatal conductance in both lettuce and spinach in response to higher [CO<sub>2</sub>], which is likely affect both water loss from the leaves and their photosynthetic activity. The results indicate a broad adverse impact of rising [CO<sub>2</sub>] on the nutritional quality of commonly consumed leafy vegetables namely, lettuce and spinach.
基金Supported by Program of Jilin Science and Technology Department(20160521008HJ)Program of Yanbian University(602016009)
文摘To decrease fertilization amount of chemical fertilizer and improve the quality of vegetable crops,spinach was taken as the test material,and the impact of different fertilizer on spinach growth and yield was studied via the manners of biological organic fertilizer and organic fertilizer + chemical fertilizer. Experimental results showed that in the formula of organic fertilizer + chemical fertilizer,chlorophyll and nitrogen contents in spinach leaves obviously increased; in the formula of only adding organic fertilizer,spinach leaf temperature,leaf width,root length,plant height and fresh weight were all better than those in the formula of organic fertilizer + chemical fertilizer,and better formulas were A_5,E_5,F_3 and I_5,in which spinach plant height in E_5 was 5. 63 times higher than G_5,root length in E_5 was 2. 67 times higher than G_5,and fresh weight in G_5 was 32. 6 times higher than G_5. By comprehensive analysis,the most suitable formula for spinach production was E_5,and the research could provide theoretic basis for fertilization amount of organic fertilizer required by spinach growth and development.
文摘Lead is a toxic and naturally occurring substance with documented neurotoxin, toxic, and long-lasting adverse health effects globally. Lead exposure can cause impaired physical and mental development in children. Exposure to high lead levels affects the intestinal tract, kidneys, joints and reproductive system in adults. This study evaluates the removal of 1500 PPM of lead from contaminated aqueous solution using Celite, Louisiana Red Clay, Charcoal, and supernatants from aqueous extracts of Mustard Green (Brassica juncea), and Spinach (Spinacea oleracea). After shaking triplicate reaction mixtures for each substrate for 22 hours at room temperature, lead removal by the five substrates were analyzed by EPA Method 6010, using Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES). Results suggest that the order of lead removal is Spinach (98%) > Charcoal (96%) > LA Red Clay (88%) > Mustard Green (87%) > Celite (4%). The study concludes that liquid substrates such as the supernatants from pureed spinach and mustard green can effectively remove lead from contaminated water.
基金Project supported by the National Natural Science Foundation of China (30470150,30800068)
文摘Chloroplasts were isolated from spinach cultured in lead chloride-present, Ce3+-administered, cerium chloride-administered lead chloride-present Hoagland's media or that of Hoagland's media. The experimental study demonstrated the effects of cerium (Ce) on distribution of light energy and photochemical activities of spinach chloroplast grown in lead (Pb)-present media. It was observed that Pb2+ significantly inhibited photosynthesis in spinach, including light absorption, energy transfer from LHCⅡ to photosystem Ⅱ, excitation energy distribution from photosystem I to photosystem Ⅱ, and transformation from light energy to electron energy and oxygen evolution of chloroplasts, and decreased spinach growth. However, Ce3+ treatment to Pb2+-present chloroplasts could obviously improve light absorption and excitation energy distribution in both photosystems and increase activity of photochemical reaction and oxygen evolution of chloroplasts. The results suggested that Ce3+ under Pb2+ stress could maintain the stability of chloroplast membrane, and improve photosynthesis of spinach chloroplast, thus promote spinach growth.
基金the USDA Speciality Crops Research Initiative (SCRI)Grant (2017-51181-26830)USDA-AMS Speciality Crop Multi-State Program (SCMP) Grant (16SCCMAR0001)USDA-CGC (5030-21000-064-009-S and 5030-21000-064-006-S) for the funding support to perform spinach genetics,genomics,and breeding research in our lab.
文摘Spinach(Spinacia oleracea)is a diploid(2n=2x=12),wind-pollinated and highly heterozygous crop.The plants are mostly dioecious,although some monoecious plants exist.Spinach is an economically important cool-season leafy vegetable crop.Demand for spinach is increasing worldwide,particularly due to its high nutritional content.Spinach is a versatile crop eaten raw or cooked and used as salads or mixed with other cuisines.This review article provides an overview of origin and domestication,genetic diversity and population structure,genetic and genomic resources,major diseases threatening spinach production,breeding progress,and synthesizing how these resources can help in spinach improvement.The rapid development of genomic and sequence resources of spinach has increased biological and genetics research and laid the foundation for adopting molecular breeding.Downy mildew is the most serious disease affecting spinach and breeding programs focus on developing cultivars resistant to continually emerging new races of downy mildew pathogens.The use of genomic and molecular resources and approaches offers promises in population improvement and hybrid development to address biotic and abiotic stresses production challenges and provide improved breeding materials and strategies against the rapidly changing pathogen races and climatic conditions.
基金Supported by Agricultural Science and Technology Innovation Project of Jilin Province
文摘Spinach is one of the main vegetable species.The study of spinach germplasm resources is beneficial to discovering excellent genes and accelerating the breeding efficiency,thereby further promoting the development of the spinach industry.In this study,the origin,propagation,collection,preservation,genetic diversity and breeding status of spinach were reviewed,and the problems and prospects of spinach germplasm resources were put forward.This paper provides reference for the research and breeding of spinach germplasm resources.
基金This research is funded by Taif University,TURSP-2020/313.
文摘In the present scenario,Deep Learning(DL)is one of the most popular research algorithms to increase the accuracy of data analysis.Due to intra-class differences and inter-class variation,image classification is one of the most difficult jobs in image processing.Plant or spinach recognition or classification is one of the deep learning applications through its leaf.Spinach is more critical for human skin,bone,and hair,etc.It provides vitamins,iron,minerals,and protein.It is beneficial for diet and is readily available in people’s surroundings.Many researchers have proposed various machine learning and deep learning algorithms to classify plant images more accurately in recent years.This paper presents a novel Convolutional Neural Network(CNN)to recognize spinach more accurately.The proposed CNN architecture classifies the spinach category,namely Amaranth leaves,Black nightshade,Curry leaves,and Drumstick leaves.The dataset contains 400 images with four classes,and each type has 100 images.The images were captured from the agricultural land located at Thirumanur,Salem district,Tamil Nadu.The proposed CNN achieves 97.5%classification accuracy.In addition,the performance of the proposed CNN is compared with Support Vector Machine(SVM),Random Forest,Visual Geometry Group 16(VGG16),Visual Geometry Group 19(VGG19)and Residual Network 50(ResNet50).The proposed provides superior performance than other models,namely SVM,Random Forest,VGG16,VGG19 and ResNet50.
文摘Extraction is most commonly used sample preparation technique in quantitative determination of all-trans-lutein in spinach. In our study RP-HPLC was applied to identify the constituents of spinach extract and to quantify all-trans-lutein content with the mobile phase consisted of acetone and water. Due to sensitivity of all-trans-lutein, we compared effects of different extraction conditions;air and nitrogen atmosphere and ethanol, ethanol with added 0.1% TBHQ and ethanol with added 0.1% BHT as solvents. Results for the all-trans-lutein content in the spinach samples and spinach samples with standard addition obtained in air and in nitrogen confirmed the induced degradation of all-trans-lutein in oxygen from the air. Ethanol containing synthetic antioxidant TBHQ under nitrogen atmosphere gave the highest yield of extraction and recovery of all-trans-lutein from spinach.
文摘Previous studies showed that rabbit muscle phosphofructokinase-1 (PFK-1) activity losses due to dilution, due to inhibition by ascorbate, and due to some lithium salts were prevented by rabbit muscle aldolase. Chicken PFK-1 and fish PFK-1 interacted with ascorbate and were inhibited, consistent with a previously proposed function that ascorbate facilitates glycogen in resting muscle by inhibiting glycolysis. This report shows that a plant enzyme, spinach aldolase, has the same ability to prevent rabbit muscle PFK-1 activity loses as rabbit muscle aldolase and in some instances it was a better protector from activity losses than rabbit aldolase. Spinach aldolase also protected chicken and fish PFK-1s from inhibitions by ascorbate and from activity losses due to dilution. Prevention of losses PFK-1 activities from animal species by a plant protein, spinach aldolase, suggests an evolutionary conservative relationship between PFK-1s and aldolases.
基金supported in part by funds from USDA-NIFA SCRI 2017-51181-26830 and a Texas A&M AgriLife Research Vegetable Seed Grant 124353-96181 to C.A.A.,as well as a Texas A&M AgriLife Research Insect-vectored Disease Seed Grants(114,190-96,210)to K.K.M.
文摘Minor alleles(MA)have been associated with disease incidence in human studies,enabling the identification of diagnostic risk factors for various diseases.However,allelic mapping has rarely been performed in plant systems.The goal of this study was to determine whether a difference in MA prevalence is a strong enough risk factor to indicate a likely significant difference in disease resistance against white rust(WR;Albugo occidentalis)in spinach(Spinacia oleracea).We used WR disease severity ratings(WR-DSRs)in a diversity panel of 267 spinach accessions to define resistant-and susceptibility-associated groups within the distribution scores and then tested the single-nucleotide polymorphism(SNP)variants to interrogate the MA prevalence in the most susceptible(MS)vs.most resistant(MR)individuals using permutation-based allelic association tests.A total of 448 minor alleles associated with WR severity were identified in the comparison between the 25%MS and the 25%MR accessions,while the MA were generally similar between the two halves of the interquartile range.The minor alleles in the MS group were distributed across all six chromosomes and made up~71%of the markers that were also strongly associated with WR in parallel performed genome-wide association study.These results indicate that susceptibility may be highly determined by the disproportionate overrepresentation of minor alleles,which could be used to select for resistant plants.Furthermore,by focusing on the distribution tails,allelic mapping could be used to identify plant markers associated with quantitative traits on the most informative segments of the phenotypic distribution.
文摘This pot-based study investigated the influence of poultry manure and 1:1 mixture of poultry manure+biochar(produced from farmyard manure[FYM]or wood),on the biomass production and concentration of heavy metals in leaves of lettuce and spinach.The concentration of mineral nitrogen(N)and soluble inorganic phosphorus(P)of soils cultivated with these vegetables was also investigated.The application of poultry manure or FYM biochar in soil as 10%(equivalent to 60 t ha^(-1),an estimated 1726.8 kg ha^(-1)N in poultry manure and 1353.9 kg ha^(-1)N in FYM)and 15%amendment(equivalent to 90 t ha^(-1),an estimated 2590.2 kg ha^(-1)N in poultry manure and 2030.8 kg ha^(-1)N in FYM)significantly decreased biomass production of lettuce as compared to control(no fertilizer added)treatment.However,mixture of poultry manure with wood-derived biochar at both application rates(i.e.,10%and 15%)and with FYM biochar at lower application rate(i.e.,10%)caused 2-3-fold increase in aboveground plant biomass and 2-14-fold increase in root biomass(p<0.05).Furthermore,as compared to control treatment,a significant~2-3-fold increase in aboveground plant biomass was also observed in response to mixture of poultry manure with wood-derived and FYM derived biochars at 10%amendment rates.As compared to control treatment,concentration of mineral N and soluble inorganic P were higher in soils of all other treatments.In spinach,amendment of poultry manure or its co-amendment with biochar of FYM significantly increased aboveground plant biomass at 7%(equivalent to 42 t ha^(-1))as compared to 3%and 5%amendment rates(equivalent to 18 and 30 t ha^(-1)respectively).The concentration of soil mineral N and soil soluble mineral P was not different between treatments.In lettuce,wood-derived biochar did not reduce concentration of heavy metals(i.e.,manganese(Mn),copper(Cu),iron,(Fe),cadmium(Cd),lead(Pb),nickel(Ni)and cobalt(Co)than FYM-derived biochar while in spinach,as compared to poultry manure,co-amendment of poultry manure with wood-derived biochar reduced concentration of heavy metals,indicating differential responses of crops to organic amendments.
文摘The effects of antimicrobial edible films containing carvacrol and cinnamaldehyde on organic baby spinach were determined via sensory analysis and changes in physical properties. Edible films made from pulp of hibiscus, apple, or carrot containing carvacrol or cinnamaldehyde at 0.5%, 1.5%, or 3% concentrations were added to organic baby spinach in plastic bags. These bags were stored at 4<span style="color:#4F4F4F;font-family:-apple-system, " font-size:16px;white-space:normal;background-color:#ffffff;"="">°</span>C for 20-24h before performing sensory evaluation and measuring changes in physical properties. A randomized block design with an affective test was used. Preference liking was evaluated based on a 9-point hedonic scale for aroma, color, freshness, mouthfeel, flavor, and overall acceptability. Additionally, panelists quantified each sample using a 5-point hedonic scale for pungency, browning, bitterness, off-odor, and sourness. The color and texture of spinach samples were measured. Edible films containing cinnamaldehyde had the highest preference liking based on aroma, color, freshness, mouthfeel, flavor, and overall acceptability than those containing carvacrol and were the most likely to be purchased by panelists;therefore, cinnamaldehyde can potentially be used as an alternative sanitization option. There were no significant (p ≤ 0.05) changes in firmness or color values between spinach treated with antimicrobial films and controls. The results provide the produce industry with options for incorporating antimicrobial films into salad bags without influencing the physical or sensory properties of baby spinach.
文摘A study was conducted in the Bekaa valley of Lebanon aiming to produce spinach leaves with treated effluent from Joub Janine plant under two growing seasons. Two experiments were laid out in a randomized complete block design. The effect of water quality on the qualitative, quantitative aspects and microbiological contamination of leaves was assessed. The results showed that the treated wastewater from Joub Janine plant was of category III. The highest mean marketable yields was recorded for T7 (4727 g·m-2) followed by T6 (3533 g·m-2) that were drip irrigated with treated wastewater. The uptake of K, Mg, Na and Cl was significantly 49.09%, 30.20%, 96.79% and 33.20%, respectively, higher in the spring than in autumn. The nitrate levels in all treatments and seasons were below the maximum level in foodstuffs as provided by the European Commission regulations. For the lipophilic fraction, there was no significant difference among treatments and also among treatments and seasons interacting together and the highest hydrophilic fraction and total phenols levels recorded for the autumn rather than the summer season. In general, pathogenic bacteria was absent on spinach leaves for all treatments and growing seasons.
文摘A pot experiment was carried out to investigate the yield of Indian spinach (Basella alba L.) and their uptake and availability of phosphorus from lime and phosphorus amended acidic soil. Four rates of lime (L) equivalent to 0, 500, 1000 and 2000 kg CaCO3 ha-1 and four rates of phosphorus (P) equivalent to 0, 50, 100, and 150 kg·P·ha-1 of TSP were applied in combinations as treatments. Dry matter yield, P concentrations in shoot and root and P uptake by Indian spinach were determined after harvesting 10 weeks old plant and soil samples were collected from each pot to measure available P by Olsen method. Both L and P and their combinations had significant (P 0.001) effects on shoot and root biomass, shoot and root P concentrations, P uptake by Indian spinach and P availability. Although lime and P increased biomass production, P concentrations of shoot and root, and its uptake by Indian spinach and available P, this effect was boosted by combining L with P applied. 1000 kg lime plus 100 kg P were adequate for plant growth. Available P was strongly and positively correlated (R2 = 0.909, P = 0.000) with P uptake by plant. Results of the present study indicated that lime and phosphorus could be used in combination to enhance plant growth.
文摘A glasshouse pot experiment was conducted to investigate cadmium (Cd) tolerance on the growth of Chinese spinach (Spinacia Oleracea L.) grown in sandy loam soil and microbial respiration after application of lherzolite (0%, 2.5% and 5%) with added Cd (0, 2.5 and 5 mg·kg-1). Soil pH tended to increase with increasing application rate of lherzolite about 1.5 units after plant harvest. Plant growth and microbial respiration in one hand decreased with the rates of Cd application in soil but on the other hand lherzolite application in soil reduced Cd toxicity and enhanced plant growth and microbial respiration. Microbial respiration of soil showed significant positive relationship with shoot and root dry weight of spinach but negative relationship of Cd concentration in plant tissue. This result indicated that application of lherzolite detoxified Cd toxicity in plant resulted in an increase plant growth and microbial respiration in Cd contaminated soil.
基金Natural Science Foundation of Beijing(No.6991004)Joint Lab between theInstitute of Soil Science, CAS, +1 种基金 Hong Kong Baptist University (No. 99122202) Federal Ministry of Education and Research, Germany.
文摘Effects of conventional and optimized water and nitrogen managements on spinach (Spinacia olerocea L.)growth and soil mineral N (Nmi.) residues were compared in an open field experiment in which water balancemethod and N recommendation with the KNS-system were included. It was shown that the conventionalwater treatment (seasonal irrigated amount: 175 mm) reduced spinach growth compared to the water balancetreatments (seasonal irrigated amount: 80 and 85 mm) at the same N supply level due to N loss throughleaching caused by excessive water supply. Although 309 kg N ha-1 was applied in the conventional Ntreatment, compared to 82 and 66 kg N ha-l in the optimum N treatments, no significant difference in cropyield was investigated between the N treatments with the same irrigation practice. N uptake in spinach andsoil residual Nmin contents were also significantly affected by the irrigation practices. The conventional watersupply not only decreased water use efficiency, but also
