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Impact of strain relaxation on the growth rate of heteroepitaxial germanium tin binary alloy
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作者 Pedram Jahandar Maksym Myronov 《Journal of Semiconductors》 EI CAS CSCD 2024年第10期35-41,共7页
The growth of high-quality germanium tin(Ge_(1–y)Sn_(y))binary alloys on a Si substrate using chemical vapor deposition(CVD)techniques holds immense potential for advancing electronics and optoelectronics application... The growth of high-quality germanium tin(Ge_(1–y)Sn_(y))binary alloys on a Si substrate using chemical vapor deposition(CVD)techniques holds immense potential for advancing electronics and optoelectronics applications,including the development of efficient and low-cost mid-infrared detectors and light sources.However,achieving precise control over the Sn concentration and strain relaxation of the Ge_(1–y)Sn_(y)epilayer,which directly influence its optical and electrical properties,remain a significant challenge.In this research,the effect of strain relaxation on the growth rate of Ge_(1–y)Sn_(y)epilayers,with Sn concentration>11at.%,is investigated.It is successfully demonstrated that the growth rate slows down by~55%due to strain relaxation after passing its critical thickness,which suggests a reduction in the incorporation of Ge into Ge_(1–y)Sn_(y)growing layers.Despite the increase in Sn concentration as a result of the decrease in the growth rate,it has been found that the Sn incorporation rate into Ge_(1–y)Sn_(y)growing layers has also decreased due to strain relaxation.Such valuable insights could offer a foundation for the development of innovative growth techniques aimed at achieving high-quality Ge_(1–y)Sn_(y)epilayers with tuned Sn concentration and strain relaxation. 展开更多
关键词 geSn germanium tin strain relaxation groupⅣsemiconductor chemical vapour deposition CVD
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The study on two-dimensional analytical model for gate stack fully depleted strained Si on silicon-germanium-on-insulator MOSFETs 被引量:3
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作者 李劲 刘红侠 +2 位作者 李斌 曹磊 袁博 《Chinese Physics B》 SCIE EI CAS CSCD 2010年第10期485-491,共7页
Based on the exact resultant solution of two-dimensional Poisson's equation in strained Si and Si1-xCex layer, a simple and accurate two-dimensional.analytical model including surface channel potential, surface chann... Based on the exact resultant solution of two-dimensional Poisson's equation in strained Si and Si1-xCex layer, a simple and accurate two-dimensional.analytical model including surface channel potential, surface channel electric field, threshold voltage and subthreshold swing for fully depleted gate stack strained Si on silicon-germanium-on-insulator (SGOI) MOSFETs has been developed. The results show that this novel structure can suppress the short channel effects (SCE), the drain-induced barrier-lowering (DIBL) and improve the subthreshold performance in nanoelectronics application. The model is verified by numerical simulation. The model provides the basic designing guidance of gate stack strained Si on SGOI MOSFETs. 展开更多
关键词 silicon-germanium-on-insulator MOSFETs strained Si short channel effects the draininduced barrier-lowering
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猪伪狂犬病病毒BJC变异株分离鉴定及gB和gE基因序列分析
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作者 娄昆鹏 李阳 +2 位作者 项伟 徐博 朱国强 《动物医学进展》 北大核心 2024年第9期20-26,共7页
为了确定伪狂犬病疑似发病猪场PRV变异株病原及其病毒分子特征,通过病理剖检、病毒分离鉴定与纯化、ST细胞TCID_(50)和小鼠LD_(50)测定,对分离鉴定的病毒gB、gE基因进行PCR扩增、回收、克隆和测序,用生物信息学分析软件Geneious Prime... 为了确定伪狂犬病疑似发病猪场PRV变异株病原及其病毒分子特征,通过病理剖检、病毒分离鉴定与纯化、ST细胞TCID_(50)和小鼠LD_(50)测定,对分离鉴定的病毒gB、gE基因进行PCR扩增、回收、克隆和测序,用生物信息学分析软件Geneious Prime对gB、gE基因进行遗传进化和同源性分析。结果显示,该分离株为PRV强毒株。该病毒在ST细胞生长良好,纯化后的病毒经测定半数组织培养感染量为10^(8.5)TCID_(50)/mL,对6周龄昆明小鼠的LD_(50)为10^(5.8)TCID_(50)。测序结果与预期的PRV gB、gE基因片段相符。遗传进化分析可知与国内代表PRV变异毒株如JS2012、HN1201、ZJ01等处于同一分支,与欧美分离株如Bartha、Kaplan、Becker等亲缘关系较远,处于不同的大分支。氨基酸序列分析表明,BJC株与国外经典毒株和国内早期分离毒株存在多个特征性位点替换,符合国内流行变异毒株特征,BJC株为PRV变异毒株。 展开更多
关键词 伪狂犬病毒病 分离鉴定 BJC株 gB、ge基因 序列分析
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Sensitivity investigation of 100-MeV proton irradiation to SiGe HBT single event effect
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作者 冯亚辉 郭红霞 +7 位作者 刘益维 欧阳晓平 张晋新 马武英 张凤祁 白如雪 马晓华 郝跃 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第1期554-562,共9页
The single event effect(SEE) sensitivity of silicon–germanium heterojunction bipolar transistor(Si Ge HBT) irradiated by 100-Me V proton is investigated. The simulation results indicate that the most sensitive positi... The single event effect(SEE) sensitivity of silicon–germanium heterojunction bipolar transistor(Si Ge HBT) irradiated by 100-Me V proton is investigated. The simulation results indicate that the most sensitive position of the Si Ge HBT device is the emitter center, where the protons pass through the larger collector-substrate(CS) junction. Furthermore, in this work the experimental studies are also carried out by using 100-Me V proton. In order to consider the influence of temperature on SEE, both simulation and experiment are conducted at a temperature of 93 K. At a cryogenic temperature, the carrier mobility increases, which leads to higher transient current peaks, but the duration of the current decreases significantly.Notably, at the same proton flux, there is only one single event transient(SET) that occurs at 93 K. Thus, the radiation hard ability of the device increases at cryogenic temperatures. The simulation results are found to be qualitatively consistent with the experimental results of 100-Me V protons. To further evaluate the tolerance of the device, the influence of proton on Si Ge HBT after gamma-ray(^(60)Coγ) irradiation is investigated. As a result, as the cumulative dose increases, the introduction of traps results in a significant reduction in both the peak value and duration of the transient currents. 展开更多
关键词 silicon–germanium heterojunction bipolar transistor(Si ge HBT) 100-Me V proton technology computer-aided design(TCAD) single event effect(SEE)
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高Ge含量Ge-As-Se三元硫系玻璃制备及其性能研究
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作者 谢双权 陈益敏 +1 位作者 沈祥 徐铁峰 《宁波大学学报(理工版)》 CAS 2024年第6期108-112,共5页
采用熔融-淬冷技术制备了4种位于传统成玻区域边缘的高Ge含量Ge-As-Se硫系玻璃,研究了极高Ge含量对其成玻能力及机械、热学、光学性能的影响.研究发现,在As含量不变的情况下,随着Ge含量增加,Ge-As-Se体系成玻能力逐渐减弱,其中Ge_(39)As... 采用熔融-淬冷技术制备了4种位于传统成玻区域边缘的高Ge含量Ge-As-Se硫系玻璃,研究了极高Ge含量对其成玻能力及机械、热学、光学性能的影响.研究发现,在As含量不变的情况下,随着Ge含量增加,Ge-As-Se体系成玻能力逐渐减弱,其中Ge_(39)As_(16)Se_(45)和Ge_(42)As_(16)Se_(42)样品呈现出完全的非晶态结构,并展现出较高的光学透过率和维氏硬度.然而,当Ge含量进一步增加时,如位于成玻区外的样品Ge_(48)As_(16)Se_(36)和Ge_(51)As_(16)Se_(33),虽然维氏硬度能继续增加,但内部结构出现不同程度的结晶,导致其不再具备良好的光学透过性.结果表明,适当提高Ge含量可在保持良好红外光透性的同时显著提高机械性能,如Ge_(42)As_(16)Se_(42)样品具备45%的红外透过率和239.7 kg·mm^(-2)的维氏硬度,这为获得更高机械强度的硫系玻璃用于红外镜头制备提供了可能. 展开更多
关键词 硫系玻璃 ge-As-Se 高锗含量 维氏硬度
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鉴别猪伪狂犬病病毒gI/gE基因部分缺失疫苗株和野毒株的TaqMan实时荧光定量PCR检测方法的建立
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作者 李倩倩 黄英 +7 位作者 陈翔鸿 宋文博 杨柳 龙云志 余道兵 梁巩 黄超 汤细彪 《中国兽医杂志》 CAS 北大核心 2024年第10期68-74,共7页
为建立一种鉴别猪伪狂犬病病毒(PRV)gI/gE基因部分缺失疫苗株和野毒株的TaqMan实时荧光定量PCR检测方法,本试验依据PRV HB-98株和HB2000株的gE和gI基因缺失片段设计特异性引物和探针,优化反应体系,绘制标准曲线,进行特异性试验和敏感性... 为建立一种鉴别猪伪狂犬病病毒(PRV)gI/gE基因部分缺失疫苗株和野毒株的TaqMan实时荧光定量PCR检测方法,本试验依据PRV HB-98株和HB2000株的gE和gI基因缺失片段设计特异性引物和探针,优化反应体系,绘制标准曲线,进行特异性试验和敏感性试验,对临床样本和疫苗样本进行检测,并与商品化试剂盒的检测结果进行对比。结果显示,本试验建立的TaqMan实时荧光定量PCR检测方法的标准曲线R^(2)为0.9971;该方法特异性强,能区分PRV与其他常见的猪病原体;敏感性高,最低检测限为10 copies/μL;对临床样本的检测结果与商品化试剂盒的检测结果一致;能够区分PRV gI/gE基因部分缺失的疫苗株和野毒株。结果表明,本试验建立了一种特异性强、灵敏度高、适用范围广的鉴别诊断PRV gI/gE基因部分缺失疫苗株和野毒株的TaqMan实时荧光定量PCR检测方法。 展开更多
关键词 猪伪狂犬病病毒(PRV) gI/ge基因部分缺失疫苗株 鉴别诊断 TaqMan实时荧光定量PCR
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Direct-bandgap electroluminescence from tensile-strained Ge/SiGe multiple quantum wells at room temperature
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作者 何超 刘智 +3 位作者 张旭 黄文奇 薛春来 成步文 《Chinese Physics B》 SCIE EI CAS CSCD 2014年第11期427-430,共4页
Tensile-strained Ge/SiGe multiple quantum wells (MQWs) were grown on a Ge-on-Si virtual substrate using ultrahigh vacuum chemical vapor deposition on an n+-Si (001) substrate. Direct-bandgap electroluminescence f... Tensile-strained Ge/SiGe multiple quantum wells (MQWs) were grown on a Ge-on-Si virtual substrate using ultrahigh vacuum chemical vapor deposition on an n+-Si (001) substrate. Direct-bandgap electroluminescence from the MQWs light emitting diode was observed at room temperature. The quantum confinement effect of the direct-bandgap transitions is in good agreement with the theoretical calculated results. The redshift mechanism of emission wavelength related to the thermal effect is discussed, 展开更多
关键词 ge multiple quantum wells tensile strain ELECTROLUMINESCENCE
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A k·p analytical model for valence band of biaxial strained Ge on(001) Si_(1-x)Ge_x
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作者 王冠宇 张鹤鸣 +2 位作者 高翔 王斌 周春宇 《Chinese Physics B》 SCIE EI CAS CSCD 2012年第5期501-507,共7页
In this paper,the dispersion relationship is derived by using the k·p method with the help of the perturbation theory,and we obtain the analytical expression in connection with the deformation potential.The calcu... In this paper,the dispersion relationship is derived by using the k·p method with the help of the perturbation theory,and we obtain the analytical expression in connection with the deformation potential.The calculation of the valence band of the biaxial strained Ge/(001)Si1-xGex is then performed.The results show that the first valence band edge moves up as Ge fraction x decreases,while the second valence band edge moves down.The band structures in the strained Ge/(001)Si 0.4 Ge 0.6 exhibit significant changes with x decreasing in the relaxed Ge along the [0,0,k] and the [k,0,0] directions.Furthermore,we employ a pseudo-potential total energy package(CASTEP) approach to calculate the band structure with the Ge fraction ranging from x = 0.6 to 1.Our analytical results of the splitting energy accord with the CASTEP-extracted results.The quantitative results obtained in this work can provide some theoretical references to the understanding of the strained Ge materials and the conduction channel design related to stress and orientation in the strained Ge pMOSFET. 展开更多
关键词 strained ge valence band k·p method dispersion relationship
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TEM and STEM Observations of a Flat Continuous Silicon-Germanium Thin Film Epitaxially Grown on Porous Silicon
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作者 Junji Yamanaka Noritaka Usami +4 位作者 Sevak Amtablian Alain Fave Mustapha Lemiti Chiaya Yamamoto Kiyokazu Nakagawa 《Journal of Materials Science and Chemical Engineering》 2017年第1期26-34,共9页
Strain-relaxed SiGe is an attractive material for use as a substrate of strained Si, in which carrier mobility is higher than that of bulk Si. The concept of this study is the use of porous Si as a sponge like substra... Strain-relaxed SiGe is an attractive material for use as a substrate of strained Si, in which carrier mobility is higher than that of bulk Si. The concept of this study is the use of porous Si as a sponge like substrate so that a SiGe lattice can relax without introducing dislocations. We produced porous Si specimens by electrochemical anodization and annealed them under a H2 atmosphere. Then, SiGe thin films were grown by gas-source molecular beam epitaxy. We observed the microstructure of the specimens using transmission electron microscopy. The result showed that we succeeded in producing a single-crys- tal continuous Si0.73Ge0.27 film with a 10% relaxation ratio and a low dislocation density on porous Si. 展开更多
关键词 Porous SILICON SILICON germanium Strain Relaxation strained SILICON Nanostructure HIGH-MOBILITY Semiconductors Transmission Electron Microscopy
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STEM MoiréObservation of Lattice-Relaxed Germanium Grown on Silicon
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作者 Junji Yamanaka Chiaya Yamamoto +4 位作者 Hiroki Nakaie Tetsuji Arai Keisuke Arimoto Kosuke O. Hara Kiyokazu Nakagawa 《Journal of Materials Science and Chemical Engineering》 2017年第1期102-108,共7页
We deposited Ge films on Si substrates by molecular beam epitaxy (MBE) method. The specimens were annealed at around 750 C using microwave- plasma heating technique which we had reported before. After these pro- cesse... We deposited Ge films on Si substrates by molecular beam epitaxy (MBE) method. The specimens were annealed at around 750 C using microwave- plasma heating technique which we had reported before. After these pro- cesses, we carried out special scanning transmission electron microscopic (STEM) observation. The moiré between the crystal lattices and the scanning lines controlled by STEM was utilized to show lattice-spacing distribution. The results exhibited that we were succeeded in forming lattice-relaxed Ge thin films. It was also recognized that this STEM moiré technique is very useful to observe lattice-spacing distribution for large area with high resolution. 展开更多
关键词 STEM Moiré LATTICE STRAIN ge on Si Plasma HEATING
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LATTICE DYNAMICS OF STRAINED Si/Ge SUPERLATTICES
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作者 ZI Jian ZHANG Kaiming XIE Xide 《Chinese Physics Letters》 SCIE CAS CSCD 1990年第5期230-233,共4页
Lattice dynamics of strained(Si)_(4)/(Ge)_(4) superlattice grown pseudomorphically on (001)-oriented Si_(1-x)Ge_(x)(0≤x≤1)substrate is investigated.In the present calculations,the effects of strain and substrate are... Lattice dynamics of strained(Si)_(4)/(Ge)_(4) superlattice grown pseudomorphically on (001)-oriented Si_(1-x)Ge_(x)(0≤x≤1)substrate is investigated.In the present calculations,the effects of strain and substrate are discussed. 展开更多
关键词 Si/ge strained SUPER
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Effect of substrate doping on threshold voltages of buried channel pMOSFET based on strained-SiGe technology
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作者 王斌 张鹤鸣 +3 位作者 胡辉勇 张玉明 周春宇 李妤晨 《Journal of Central South University》 SCIE EI CAS 2014年第6期2292-2297,共6页
The effect of substrate doping on the threshold voltages of buried channel pMOSFET based on strained-SiGe technology was studied.By physically deriving the models of the threshold voltages,it is found that the layer w... The effect of substrate doping on the threshold voltages of buried channel pMOSFET based on strained-SiGe technology was studied.By physically deriving the models of the threshold voltages,it is found that the layer which inversely occurs first is substrate doping dependent,giving explanation for the variation of plateau observed in the C-V characteristics of this device,as the doping concentration increases.The threshold voltages obtained from the proposed model are-1.2805 V for buried channel and-2.9358 V for surface channel at a lightly doping case,and-3.41 V for surface channel at a heavily doping case,which agrees well with the experimental results.Also,the variations of the threshold voltages with several device parameters are discussed,which provides valuable reference to the designers of strained-SiGe devices. 展开更多
关键词 buried pMOSFET strained Sige plateau threshold voltage substrate doping ge fraction
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伪狂犬病病毒Ea株TK^-/gE^-/gp63^-突变株的构建及其生物学特性研究 被引量:16
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作者 刘正飞 陈焕春 +2 位作者 何启盖 周复春 方六荣 《微生物学报》 CAS CSCD 北大核心 2002年第3期370-374,共5页
Using pseudorabies virus Ea strain as material,we inserted LacZ gene expression cassette into gE gene.After blue plaque and plaque purification,a recombinant virus PRVEa TK+-/gE+-/LacZ++ generated.Utilizing EcoR I sit... Using pseudorabies virus Ea strain as material,we inserted LacZ gene expression cassette into gE gene.After blue plaque and plaque purification,a recombinant virus PRVEa TK+-/gE+-/LacZ++ generated.Utilizing EcoR I site in LacZ gene, digested PRVEa TK+-/gE+-/LacZ++ genome DNA was cotransfected into PK-15 cells with plasmid pFBBS,then PRVEa TK+-/gE+-/gp63+- generated after plaque purification.Four pairs of primers amplification demonstrated the virus was pure TK+-/gE+-/gp63+- mutant virus.PCR product sequence indicates there were 205bp deletion in TK gene;1247bp deletion in gE,gp63 and intergenic region of PRVEa TK+-/gE+-/gp63+- mutant virus genome DNA.Inoculation to Balb/C mice with PRVEa TK+-/gE+-/gp63+- indicates the virulence is reduced greatly. 展开更多
关键词 伪狂犬病病毒EA株 TK^-/ge^-/gp63^-突变株 生物学特性
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伪狂犬病病毒鄂A株TK^-/gE^-/gI^-基因缺失疫苗的安全性和保护力研究 被引量:17
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作者 刘正飞 陈焕春 +2 位作者 吴斌 何启盖 秦永辉 《畜牧兽医学报》 CAS CSCD 北大核心 2004年第1期70-73,共4页
对PrVEaTK-/gE-/gI-基因缺失疫苗的安全性和保护力进行了系统的研究。试验表明,该基因缺失疫苗105 0TCID50和106 0TCID50病毒剂量对妊娠母猪、新生仔猪和育肥猪均是安全的,并可保护妊娠母猪抵抗107 1TCID50强毒的攻击。新生仔猪免疫30d... 对PrVEaTK-/gE-/gI-基因缺失疫苗的安全性和保护力进行了系统的研究。试验表明,该基因缺失疫苗105 0TCID50和106 0TCID50病毒剂量对妊娠母猪、新生仔猪和育肥猪均是安全的,并可保护妊娠母猪抵抗107 1TCID50强毒的攻击。新生仔猪免疫30d后,gE鉴别ELISA试验表明,PrVEaTK-/gE-/gI-免疫猪不产生针对gE的抗体。育肥猪在二次免疫后中和抗体水平显著升高。以105 0TCID50和106 0TCID50疫苗病毒接种家兔、猫和奶山羊等非靶动物,结果非靶动物未出现精神异常或死亡现象,说明该基因缺失疫苗具有极高的生物安全性。 展开更多
关键词 伪狂犬病 病毒 鄂A株 TK^-/ge^-/gI^-基因 基因缺失 疫苗 安全性 保护力
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有机锗Ge-132对氧自由基和由羟自由基诱导的脂质过氧化的影响 被引量:88
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作者 王巨存 邢国胜 +3 位作者 胡文铎 朱铁梁 王倩 赵华 《中国药学杂志》 CAS CSCD 北大核心 1994年第1期23-25,共3页
有机锗Ge-132对邻苯二酚自氧化过程中产生的超氧阴离子自由基具有清除作用,但比抗坏血酸弱;Ge-132对由Fenton反应产生的羟自由基具有清除作用,对羟自由基诱导的大鼠肝匀浆脂质过氧化具有抑制作用。在清除羟自由基... 有机锗Ge-132对邻苯二酚自氧化过程中产生的超氧阴离子自由基具有清除作用,但比抗坏血酸弱;Ge-132对由Fenton反应产生的羟自由基具有清除作用,对羟自由基诱导的大鼠肝匀浆脂质过氧化具有抑制作用。在清除羟自由基抗脂质过氧化方面,Ge-132的作用强于甘露醇。 展开更多
关键词 ge-132 有机锗 自由基 过氧化脂质
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尿中锗的测定及口服Ge—132尿药的药物动力学 被引量:4
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作者 陈雁君 王宁 +1 位作者 上官国强 陈亚民 《中国医院药学杂志》 CAS CSCD 北大核心 1993年第3期103-105,共3页
本文用胶束增溶苯基荧光酮分光光度法测定了尿中锗,研究了成人口服Ge-132后尿药的药动学。测尿中锗的平均回收率为98.1~104.3%,不同浓度测定结果的日内和日间变异系数均小于5%,检出限为0.012μg/ml。主要药动学参数(?)K_a0.451h^(-1)、... 本文用胶束增溶苯基荧光酮分光光度法测定了尿中锗,研究了成人口服Ge-132后尿药的药动学。测尿中锗的平均回收率为98.1~104.3%,不同浓度测定结果的日内和日间变异系数均小于5%,检出限为0.012μg/ml。主要药动学参数(?)K_a0.451h^(-1)、K0.292h^(-1)、t_(max)2.73h。 展开更多
关键词 锗132 药物动力学 分光光度法
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伪狂犬病病毒Fa株gE基因在大肠杆菌中的表达及gE-ELISA鉴别诊断方法的建立 被引量:4
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作者 阳爱国 郭万柱 +2 位作者 赵银丽 徐志文 王小玉 《中国兽医学报》 CAS CSCD 北大核心 2007年第3期289-291,304,共4页
试验以pPGE DNA为模板,用1对分别含有EcoRⅠ和Bam HⅠ酶切位点的伪狂犬病病毒gE基因特异性引物,扩增出约1.7kb的含完整gE基因的DNA片段;目的片段经EcoRⅠ和Bam HⅠ双酶切后,插入原核表达载体pBV220得到重组表达质粒pBVgE,并转化大肠杆菌... 试验以pPGE DNA为模板,用1对分别含有EcoRⅠ和Bam HⅠ酶切位点的伪狂犬病病毒gE基因特异性引物,扩增出约1.7kb的含完整gE基因的DNA片段;目的片段经EcoRⅠ和Bam HⅠ双酶切后,插入原核表达载体pBV220得到重组表达质粒pBVgE,并转化大肠杆菌DH5α;对温敏诱导表达所获产物进行SDS-PAGE、Western-Blot和琼脂双扩散检测。结果表明,gE糖蛋白得到了高效表达,表达产物约占总蛋白的17%。为了鉴别伪狂犬病疫苗免疫猪和自然感染猪,用表达纯化的gE蛋白为抗原,建立了GE-ELISA方法。选定的反应条件包括GE抗原包被浓度为6.6mg/L,血清最适稀释度为1∶40。测试结果:与PRV、HCV、PRRSV、JEV、SA215阳性血清呈阴性反应,而与PRV标准阳性血清呈阳性反应。这一结果表明该法特异性强、敏感性高、重复性好,可用于猪伪狂犬病的临床诊断,尤其疫苗免疫猪和自然感染猪的鉴别。 展开更多
关键词 伪狂犬病病毒 ge基因 ge—ELISA
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伪狂犬病病毒BJ/YT株毒力相关基因gE和TK序列分析 被引量:15
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作者 钟承 刘蕾 +5 位作者 张乐天 王巨实 王安琦 陆宝生 杨万莲 吕艳丽 《中国畜牧兽医》 CAS 北大核心 2014年第11期73-79,共7页
本试验对2012年从北京地区约克夏犬体内分离得到的伪狂犬病病毒(PRV)BJ/YT株主要毒力基因gE和TK的分子特征和进化关系进行了分析。结果显示,BJ/YT株gE基因与参考序列的核苷酸和氨基酸同源性分别为98.9%~100.0%和98.3%~100.0%;TK基... 本试验对2012年从北京地区约克夏犬体内分离得到的伪狂犬病病毒(PRV)BJ/YT株主要毒力基因gE和TK的分子特征和进化关系进行了分析。结果显示,BJ/YT株gE基因与参考序列的核苷酸和氨基酸同源性分别为98.9%~100.0%和98.3%~100.0%;TK基因核苷酸和氨基酸同源性分别为99.2%~99.9%和99.0%~100.0%。BJ/YT株与同期河北猪源分离株进化关系较近,各毒株之间同源性高,并且存在一致的核苷酸突变位点;与其他参考序列比对分析结果显示,BJ/YT株主要毒力基因存在变异位点,但这些位点均不在已知的主要功能区和抗原表位区内。因此,从分子流行病学角度来看,BJ/YT毒株是近年北京及其周边地区PRV流行毒株,但gE和TK基因的变异对流行毒株的毒力无明显影响。 展开更多
关键词 伪狂犬病病毒 BJ/YT株 ge基因 TK基因 序列分析
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特超强毒648株马立克氏病病毒的囊膜糖蛋白gE基因的克隆和序列比较 被引量:6
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作者 刘岳龙 崔治中 +2 位作者 何良梅 朱素娟 秦爱建 《微生物学报》 CAS CSCD 北大核心 2001年第2期155-161,共7页
鸡马立克氏病毒特超强毒 (vv+MDV) 648株的囊膜糖蛋白 gE基因经PCR扩增并克隆入pUC1 8载体。 648株gE基因经核酸序列分析测定 ,全长为 1 494碱基。所编码的蛋白具有跨膜糖蛋白的一些特征。它含有 8个潜在的糖基化位点、N端有一段疏水区... 鸡马立克氏病毒特超强毒 (vv+MDV) 648株的囊膜糖蛋白 gE基因经PCR扩增并克隆入pUC1 8载体。 648株gE基因经核酸序列分析测定 ,全长为 1 494碱基。所编码的蛋白具有跨膜糖蛋白的一些特征。它含有 8个潜在的糖基化位点、N端有一段疏水区 (1~ 1 9aa)所构成的信号肽、C端有一段疏水区 (391~ 41 9aa)所构成的膜锚着序列。经 648株 (vv+MDV)和马立克氏病毒强毒 (vMDV)GA株的 gE相比较 ,在MDV血清 1型中 gE是较为保守的 ,二者仅有 2个核苷酸的差异 (第 51 2位、第 1 472位 ) ,并导致了有二个相应的氨基酸的改变 (第1 71位Leu/Pro、第 491位Arg/Lys)。 展开更多
关键词 糖蛋白ge 克隆 序列比较 鸡马立克氏病病毒 囊膜
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贵州半边街锗锌矿床锗的富集特征及其地质意义
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作者 程涌 周家喜 +1 位作者 孙国涛 黄智龙 《岩石学报》 SCIE EI CAS CSCD 北大核心 2024年第1期43-59,共17页
锗(Ge)属于战略性关键矿产资源,是众多高新技术领域的重要原材料。Ge主要以共/伴生形式产出于Zn、Pb和Cu等热液贱金属矿床和煤矿床中,目前全球Ge资源主要来源于铅锌矿的副产品。近期取得较大找矿突破的半边街锌矿床(7.70Mt矿石,Zn平均品... 锗(Ge)属于战略性关键矿产资源,是众多高新技术领域的重要原材料。Ge主要以共/伴生形式产出于Zn、Pb和Cu等热液贱金属矿床和煤矿床中,目前全球Ge资源主要来源于铅锌矿的副产品。近期取得较大找矿突破的半边街锌矿床(7.70Mt矿石,Zn平均品位5.1%)伴生有超大型规模的金属Ge(>900t@110×10^(-6)),然而Ge超常富集的机制不清。本次研究利用LA-ICPMS对闪锌矿进行了微区原位微量元素组成和Mapping分析,以揭示Ge等元素的富集特征、赋存状态和替代方式,探讨Ge的富集机制。分析结果表明,半边街矿床闪锌矿中高含量(平均值>100×10^(-6))微量元素有Fe、Pb、Ge和Cd等,较高含量(平均值100×10^(-6)~10×10^(-6))的有Mn、Ga、Sn和Tl,以及较低含量(平均值<10×10^(-6))的有Ag、Cu、In和Sb。相比于各类型铅锌矿床,半边街闪锌矿Ge超常富集(274×10^(-6)~1938×10^(-6),平均为1055×10^(-6),达到1000倍富集)。该矿床中Ge以类质同象或纳米颗粒形式赋存于闪锌矿中。微量元素摩尔量相关性图揭示Ge替代Zn的方式以Ge^(4+)+Pb^(2+)3Zn^(2+)为主,其次为nGe^(4+)+Mn 2+(2n+1)Zn^(2+)和nGe^(4+)+2Tl^(3+)(2n+3)Zn^(2+)。综合分析认为,半边街矿床Ge可能来源于相对富Ge的前寒纪基底;富有机质的盆地流体在Ge的搬运中发挥重要作用;Ge、Cd等稀散元素的共生分异制约了Ge的最终超常富集。因此,半边街矿床中Ge的超常富集是“源、运、聚”复杂过程多要素耦合作用的结果。 展开更多
关键词 微量元素 闪锌矿 锗(ge) 替代机制 半边街锗锌矿床
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