Objective:To determine the protective effect of co-enzyme Q10(CoQ10)on testicular tissue and sperm parameters in male rats treated with SunsetYellow FCF.Methods:Sixty male Sprague-Dawley rats were randomly divided int...Objective:To determine the protective effect of co-enzyme Q10(CoQ10)on testicular tissue and sperm parameters in male rats treated with SunsetYellow FCF.Methods:Sixty male Sprague-Dawley rats were randomly divided into 6 groups of the control,CoQ10(10 mg/kg/day),low dose of Sunset Yellow(2.5 mg/kg),high dose of Sunset Yellow(70 mg/kg),low dose of Sunset Yellow(2.5 mg/kg)plus CoQ10,and high dose of Sunset Yellow(70 mg/kg)plus CoQ10.The drugs were administered via daily oral gavages for 6 weeks.At the end of the experiment,sperm analysis,stereological and histological assessments of the testis were carried out.Results:The normal morphology(by 41.1%)and progressive spermatozoa(by 74.8%),testicle volume(by 33.4%),lumen volume(by 38.3%),interstitial tissue volume(by 44.7%),seminiferous tubule volume(by 40.7%),and number of spermatogonia(by 53.9%)and Leydig cells(by 70.7%)reduced in the rats that received high doses of Sunset Yellow in comparison to the control group.Nonetheless,all these alterations were recovered by CoQ10 treatment in the CoQ10 plus high dose of Sunset Yellow group.Furthermore,low doses of Sunset Yellow did not affect different parameters of the testis and sperm.Conclusions:CoQ10 could,to some extent,prevent structural changes of the testis induced by the high dose of SunsetYellow.展开更多
The use of dyes such as tartrazine (E102) and sunset yellow (E102) in food, beverages and health products for technological and commercial purposes is common. The adverse effects caused by these dyes, such as allergie...The use of dyes such as tartrazine (E102) and sunset yellow (E102) in food, beverages and health products for technological and commercial purposes is common. The adverse effects caused by these dyes, such as allergies and hyperactivity disorder have been reported, especially in children. In the present study, a chromatographic method was developed and validated for simultaneous determination of tartrazine and sunset yellow. The chromatographic separation was performed on a Lichrocart<sup>®</sup> C18 column (125 × 4.6 mm;5 μm) with a security Guard-C18 column (4 × 2.0 mm, 5 μm;Phenomenex, Torrance, CA, USA) maintained at 30°C. The mobile phase consisted of a mixture of acetonitrile/ammonium acetate buffer pH 6.8 in gradient mode with a flow rate of 1 mL/min. The injection volume was 10 μL. The detection wavelength was set at 455 nm. The parameters of specificity, linearity, precision, repeatability, accuracy and sensitivity were examined for validation. The developed method is linear in the range of 1 μg/mL to 100 μg/mL with a R<sup>2</sup>> 0.998. The intra-day and inter-day precisions (RSD) were less than 0.6% and 3.1% respectively. The detection limit was 0.03 μg/mL and the quantification limit was 0.1 μg/mL. The retention time of tartrazine was 2.86 min, while sunset yellow was detected at 5.67 min. A simple, rapid, accurate and robust HPLC/UV-Visible method was developed and validated for simultaneous identification and quantification of tartrazine and sunset yellow. This developed method was successfully applied for the simultaneous determination of tartrazine and sunset yellow in soft drinks sold in Benin.展开更多
The removal effect of sunset yellow by the activated carbon prepared by modified straw was studied, and the effects of adsorption temperature, adsorption time, adsorption capacity, solution salinity and solution pH on...The removal effect of sunset yellow by the activated carbon prepared by modified straw was studied, and the effects of adsorption temperature, adsorption time, adsorption capacity, solution salinity and solution pH on the removal effect of sunset yellow were analyzed. After being soaked in acid and alkali, the stalks and leaves of the straw were carbonized and modified at 200-700 ℃. The results show that the adsorption effect of the leaves soaked in alkali was the best when the carbonization temperature was 700 ℃. As the adsorption temperature was 30-40 ℃, the adsorption effect of sunset yellow was the best. With the increase of adsorption time, the removal rate of sunset yellow first increased, then decreased, and finally rose again. The first critical point was about 30 min. When the dosage of activated carbon was 0.7 g, the adsorption effect of 50 ml of sunset yellow solution was the best. The optimal solution salinity was 60%. pH had a small effect on the adsorption of sunset yellow, showing a fluctuating trend.展开更多
[Objective] The aim was to explore an optimized method to measure the artificial pigment by means of high performance liquid chromatograph.[Method] The gradient elution technique of HPLC was used to isolate and analyz...[Objective] The aim was to explore an optimized method to measure the artificial pigment by means of high performance liquid chromatograph.[Method] The gradient elution technique of HPLC was used to isolate and analyze tartrazine and sunset yellow in the cereals.The pre-treatment temperature,ultrasonic time,the column temperature and the elution conditions were studied,and the best experimental conditions were confirmed.[Result] At 40 ℃,the optimum chromatography analysis condition was moving phase,with pH(6.8),methanol/water of acetic acid-ammonium acetate(0.02 mol/L),and column temperature(35 ℃).Gradient elution condition was 20%-35% methanol,3%/min;35%-98%,6%/min;98%,8 min.The content of tartrazine and sunset yellow in cereal was tested precisely by the means of HPLC.The average recoveries of standard addition were 97.9% and 100.2%,and the detection limit were 0.02 mg/kg and 0.03 mg/kg.[Conclusion] This method shortened the analysis time,improved the detection sensitivity and the resolution of artificial color.展开更多
A method to extract and analyze Sudan I present in Sunset Yellow FCF (SYF) products was developed and validated. The method included the simple extraction of Sudan I from the SYF product using water, acetonitrile, and...A method to extract and analyze Sudan I present in Sunset Yellow FCF (SYF) products was developed and validated. The method included the simple extraction of Sudan I from the SYF product using water, acetonitrile, and ethyl acetate and high-performance liquid chromatography (HPLC) analysis with isocratic elution using acetonitrile:water (7:3) with a photodiode array detector at 485 nm. This method was found to remove most of the excess SYF colorant and other impurities before injection to the HPLC instrument, making it easy to maintain precision control in routine laboratory tests for Sudan I in the SYF colorant. The detection limit of Sudan I in SYF products was 0.2 μg/g. A survey conducted to determine Sudan I in 13 commercial SYF samples from Japanese manufacturers from 1970 to 2010 showed that the levels of Sudan I ranged from 0.3 to 1.9 μg/g in products manufactured from 1970 to 1996 and were below the limit of detection in products manufactured after 2005.展开更多
An electrochemical method for fast detecting the concentration of sunset yellow FCF in wine samples was developed in this study. The sensor based on imprinted films which fabricated by electropolymerization of pyrrole...An electrochemical method for fast detecting the concentration of sunset yellow FCF in wine samples was developed in this study. The sensor based on imprinted films which fabricated by electropolymerization of pyrrole on a glassy carbon electrode in the presence of sunset yellow FCF as the template. Comparing to the polypyrrole non-imprinted modified (NIP) electrode, the polypyrrole molecularly imprinted polymer (MIP) electrode improved the electrochemical performance of the sensor significantly. The peak current at about 0.26 V was linear with the concentration of sunset yellow FCF from 0.4 to 2 μM and from 2 to 8 μM. It can be used for more than 10 times to maintain a stable response result. The sensor had the good selectivity on sunset yellow FCF, amaranth and tartrazine, which the selection factors were 1.00, 0.80 and 0.85,respectively.展开更多
An untried,low cost,locally available biosorbent for its anionic dye removal capacity from aqueous solution was investigated.Powder prepared from peanut hull had been used for biosorption of three anionic dyes,amarant...An untried,low cost,locally available biosorbent for its anionic dye removal capacity from aqueous solution was investigated.Powder prepared from peanut hull had been used for biosorption of three anionic dyes,amaranth (Am),sunset yellow (SY) and fast green FCF (FG).The effects of various experimental parameters (e.g.initial pH and dye concentration,sorbent dosage,particle size,ion strength,contact time etc.) were examined and optimal experimental conditions were decided.At initial pH 2.0,three dyes studied could be removed effectively.When the dye concentration was 50 mg·L -1 ,the percentages of dyes sorbed was 95.5% in Am,91.3% in SY and 94.98% in FG,respectively.The ratios of dyes sorbed had neared maximum values in all three dyes when sorbent dose of 5.0 g·L -1 and the sorbent particle size in 80～100 mesh was used.The increasing the ion strength of solution caused the decrease in biosorption percentages of dyes.The equilibrium values arrived at about 36 hour for all three dyes.The isothermal data of biosorption followed the Langmuir and Freundlich models.The biosorption processes conformed the pseudo-first-order rate kinetics.The results indicated that powdered peanut hull was an attractive candidate for removing anionic dyes from dye wastewater.展开更多
文摘Objective:To determine the protective effect of co-enzyme Q10(CoQ10)on testicular tissue and sperm parameters in male rats treated with SunsetYellow FCF.Methods:Sixty male Sprague-Dawley rats were randomly divided into 6 groups of the control,CoQ10(10 mg/kg/day),low dose of Sunset Yellow(2.5 mg/kg),high dose of Sunset Yellow(70 mg/kg),low dose of Sunset Yellow(2.5 mg/kg)plus CoQ10,and high dose of Sunset Yellow(70 mg/kg)plus CoQ10.The drugs were administered via daily oral gavages for 6 weeks.At the end of the experiment,sperm analysis,stereological and histological assessments of the testis were carried out.Results:The normal morphology(by 41.1%)and progressive spermatozoa(by 74.8%),testicle volume(by 33.4%),lumen volume(by 38.3%),interstitial tissue volume(by 44.7%),seminiferous tubule volume(by 40.7%),and number of spermatogonia(by 53.9%)and Leydig cells(by 70.7%)reduced in the rats that received high doses of Sunset Yellow in comparison to the control group.Nonetheless,all these alterations were recovered by CoQ10 treatment in the CoQ10 plus high dose of Sunset Yellow group.Furthermore,low doses of Sunset Yellow did not affect different parameters of the testis and sperm.Conclusions:CoQ10 could,to some extent,prevent structural changes of the testis induced by the high dose of SunsetYellow.
文摘The use of dyes such as tartrazine (E102) and sunset yellow (E102) in food, beverages and health products for technological and commercial purposes is common. The adverse effects caused by these dyes, such as allergies and hyperactivity disorder have been reported, especially in children. In the present study, a chromatographic method was developed and validated for simultaneous determination of tartrazine and sunset yellow. The chromatographic separation was performed on a Lichrocart<sup>®</sup> C18 column (125 × 4.6 mm;5 μm) with a security Guard-C18 column (4 × 2.0 mm, 5 μm;Phenomenex, Torrance, CA, USA) maintained at 30°C. The mobile phase consisted of a mixture of acetonitrile/ammonium acetate buffer pH 6.8 in gradient mode with a flow rate of 1 mL/min. The injection volume was 10 μL. The detection wavelength was set at 455 nm. The parameters of specificity, linearity, precision, repeatability, accuracy and sensitivity were examined for validation. The developed method is linear in the range of 1 μg/mL to 100 μg/mL with a R<sup>2</sup>> 0.998. The intra-day and inter-day precisions (RSD) were less than 0.6% and 3.1% respectively. The detection limit was 0.03 μg/mL and the quantification limit was 0.1 μg/mL. The retention time of tartrazine was 2.86 min, while sunset yellow was detected at 5.67 min. A simple, rapid, accurate and robust HPLC/UV-Visible method was developed and validated for simultaneous identification and quantification of tartrazine and sunset yellow. This developed method was successfully applied for the simultaneous determination of tartrazine and sunset yellow in soft drinks sold in Benin.
基金Supported by the Public Welfare Technology Research Project of Zhejiang Province (LGF20D060001)the National Natural Science Foundation of China (U1809214)。
文摘The removal effect of sunset yellow by the activated carbon prepared by modified straw was studied, and the effects of adsorption temperature, adsorption time, adsorption capacity, solution salinity and solution pH on the removal effect of sunset yellow were analyzed. After being soaked in acid and alkali, the stalks and leaves of the straw were carbonized and modified at 200-700 ℃. The results show that the adsorption effect of the leaves soaked in alkali was the best when the carbonization temperature was 700 ℃. As the adsorption temperature was 30-40 ℃, the adsorption effect of sunset yellow was the best. With the increase of adsorption time, the removal rate of sunset yellow first increased, then decreased, and finally rose again. The first critical point was about 30 min. When the dosage of activated carbon was 0.7 g, the adsorption effect of 50 ml of sunset yellow solution was the best. The optimal solution salinity was 60%. pH had a small effect on the adsorption of sunset yellow, showing a fluctuating trend.
基金Supported by Heilongjiang Natural Science Fund(B200911)Young Teacher' Scientific Research Support Program in Qiqihaer University(2010k-M17)Qiqihaer City Industrial Research and Development Program(GYGG2010-06-1)
文摘[Objective] The aim was to explore an optimized method to measure the artificial pigment by means of high performance liquid chromatograph.[Method] The gradient elution technique of HPLC was used to isolate and analyze tartrazine and sunset yellow in the cereals.The pre-treatment temperature,ultrasonic time,the column temperature and the elution conditions were studied,and the best experimental conditions were confirmed.[Result] At 40 ℃,the optimum chromatography analysis condition was moving phase,with pH(6.8),methanol/water of acetic acid-ammonium acetate(0.02 mol/L),and column temperature(35 ℃).Gradient elution condition was 20%-35% methanol,3%/min;35%-98%,6%/min;98%,8 min.The content of tartrazine and sunset yellow in cereal was tested precisely by the means of HPLC.The average recoveries of standard addition were 97.9% and 100.2%,and the detection limit were 0.02 mg/kg and 0.03 mg/kg.[Conclusion] This method shortened the analysis time,improved the detection sensitivity and the resolution of artificial color.
文摘A method to extract and analyze Sudan I present in Sunset Yellow FCF (SYF) products was developed and validated. The method included the simple extraction of Sudan I from the SYF product using water, acetonitrile, and ethyl acetate and high-performance liquid chromatography (HPLC) analysis with isocratic elution using acetonitrile:water (7:3) with a photodiode array detector at 485 nm. This method was found to remove most of the excess SYF colorant and other impurities before injection to the HPLC instrument, making it easy to maintain precision control in routine laboratory tests for Sudan I in the SYF colorant. The detection limit of Sudan I in SYF products was 0.2 μg/g. A survey conducted to determine Sudan I in 13 commercial SYF samples from Japanese manufacturers from 1970 to 2010 showed that the levels of Sudan I ranged from 0.3 to 1.9 μg/g in products manufactured from 1970 to 1996 and were below the limit of detection in products manufactured after 2005.
文摘An electrochemical method for fast detecting the concentration of sunset yellow FCF in wine samples was developed in this study. The sensor based on imprinted films which fabricated by electropolymerization of pyrrole on a glassy carbon electrode in the presence of sunset yellow FCF as the template. Comparing to the polypyrrole non-imprinted modified (NIP) electrode, the polypyrrole molecularly imprinted polymer (MIP) electrode improved the electrochemical performance of the sensor significantly. The peak current at about 0.26 V was linear with the concentration of sunset yellow FCF from 0.4 to 2 μM and from 2 to 8 μM. It can be used for more than 10 times to maintain a stable response result. The sensor had the good selectivity on sunset yellow FCF, amaranth and tartrazine, which the selection factors were 1.00, 0.80 and 0.85,respectively.
文摘An untried,low cost,locally available biosorbent for its anionic dye removal capacity from aqueous solution was investigated.Powder prepared from peanut hull had been used for biosorption of three anionic dyes,amaranth (Am),sunset yellow (SY) and fast green FCF (FG).The effects of various experimental parameters (e.g.initial pH and dye concentration,sorbent dosage,particle size,ion strength,contact time etc.) were examined and optimal experimental conditions were decided.At initial pH 2.0,three dyes studied could be removed effectively.When the dye concentration was 50 mg·L -1 ,the percentages of dyes sorbed was 95.5% in Am,91.3% in SY and 94.98% in FG,respectively.The ratios of dyes sorbed had neared maximum values in all three dyes when sorbent dose of 5.0 g·L -1 and the sorbent particle size in 80～100 mesh was used.The increasing the ion strength of solution caused the decrease in biosorption percentages of dyes.The equilibrium values arrived at about 36 hour for all three dyes.The isothermal data of biosorption followed the Langmuir and Freundlich models.The biosorption processes conformed the pseudo-first-order rate kinetics.The results indicated that powdered peanut hull was an attractive candidate for removing anionic dyes from dye wastewater.
