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Soil properties and not plant factors affect both abundant and rare microbial taxa after thinning in a mixed stand of Cunninghamia lanceolata and Sassafras tzumu
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作者 Wanxia Peng Tongqing Song +5 位作者 Hu Du Feng Wang Fuping Zeng Yang Wang Shulin Chen Yinzhu Chen 《Journal of Forestry Research》 SCIE EI CAS CSCD 2024年第2期81-94,共14页
Thinning is an effective management step for sustainable forest development,yet less attention is paid to the restoration of soil microbiota after thinning.In this study,both abundant and rare soil microbial communiti... Thinning is an effective management step for sustainable forest development,yet less attention is paid to the restoration of soil microbiota after thinning.In this study,both abundant and rare soil microbial communities(i.e.,bacterial,fungal),were evaluated under various thinning treatments in a mixed stand of Cunninghamia lanceolata and Sassafras tzumu using Mi Seq sequencing.Thinning did not significantly change either abundant or rare bacterial and fungal community composition,but affected their alpha diversity.The Shannon–Wiener indexes of rare fungal taxa under medium thinning were significantly lower than in the light thinning(P<0.05 level).Xanthobacteraceae dominated the abundant bacterial taxa,and Saitozyma and Mortierlla the abundant fungal taxa.The most common rare bacterial taxa varied;there was no prevalent rare fungal taxa under different thinnings.In addition,soil available nitrogen,total phosphorus,and p H had significant effects on rare bacterial taxa.Nutrients,especially available phosphorus,but not nitrogen,affected abundant and rare soil fungi.The results indicate that soil properties rather than plant factors affect abundant and rare microbial communities in soils of mixed stands.Thinning,through mediating soil properties,influences both abundant and rare bacterial and fungal communities in the mixed C.lanceolata and S.tzumu stand. 展开更多
关键词 Diversity Abundant taxa Rare taxa THINNING Mixed stand
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Response and assembly of abundant and rare taxa in Zaopei under different combination patterns of Daqu and pit mud:from microbial ecology to Baijiu brewing microecosystem
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作者 Yu Mu Jun Huang +5 位作者 Rongqing Zhou Suyi Zhang Hui Qin Hanlan Tang Qianglin Pan Huifang Tang 《Food Science and Human Wellness》 SCIE CSCD 2024年第3期1439-1452,共14页
The quality and aroma of strong-flavor Baijiu are mainly dependent on Daqu,pit mud(PM),and the interaction of both.However,little is known about how their combination patterns affect the microbiome and metabolome of Z... The quality and aroma of strong-flavor Baijiu are mainly dependent on Daqu,pit mud(PM),and the interaction of both.However,little is known about how their combination patterns affect the microbiome and metabolome of Zaopei,especially the metabolic function of rare taxa.Here,an experiment on industrial size was designed to assess the effects of 6 combinations(3 kinds of Daqu×2 kinds of PM)on the composition and assembly of different taxa,as well as the flavor profile.The results showed that Zaopei's microbiota was composed of a few abundant taxa and enormous rare taxa,and rare bacterial and abundant fungal subcommunities were significantly affected by combination patterns.The assembly processes of abundant/rare taxa and bacterial/fungal communities were distinct,and environmental changes mediated the balance between stochastic and deterministic processes in rare bacteria assembly.Furthermore,specific combination patterns improved the flavor quality of Zaopei by enhancing the interspecies interaction,which was closely related to rare taxa,especially rare bacteria.These findings highlighted that rare bacteria might be the keystone in involving community interaction and maintaining metabolic function,which provided a scientific foundation for better understanding and regulating the brewing microbiota from the viewpoint of microbial ecology. 展开更多
关键词 Bioaugmented Daqu Artificial pit mud Abundant and rare taxa Community assembly Metabolic function
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Sporting Usefulness Potential of Nine Local Taxa of Grasses for Lawns in the Agro-Ecological Zone with Bimodal Rainfall in Cameroon
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作者 Meboma Zanga Yanique Lionel Godswill Ntsomboh Ntsefong +2 位作者 Temegne Nono Carine Tonfack Libert Brice Youmbi Emmanuel 《American Journal of Plant Sciences》 2023年第8期845-856,共9页
The Cameroonian flora has a very high biodiversity that remains largely unexplored. It contains many grasses that can be used for sports lawns, but are currently underutilized. In order to contribute to the improvemen... The Cameroonian flora has a very high biodiversity that remains largely unexplored. It contains many grasses that can be used for sports lawns, but are currently underutilized. In order to contribute to the improvement of the quality of sports lawns, nine local grass taxa from the agro-ecological zone with bimodal rainfall in Cameroon were evaluated for their potential for sporting use in natural lawns. The method of the Study and Control Group for Varieties and Seeds was used to evaluate the sports lawn characteristics of the taxa. The resistance to trampling and pulling, the density of the lawn, and the aesthetic appearance, which are factors in calculating the sports index, were monitored at two sites, including the experimental space at the University of Yaounde 1 in the Center region and Kagnol 2 in the East region, from January 2017 to January 2020. The monthly lawn characteristic data allowed for the differentiation between the means and the determination of the sports index of the taxa compared to commercialized lawn varieties. The sports index of the evaluated taxa varied: Cynodon dactylon (6.989), Eleusine indica (6.338), Sporobolus natalensis (6.301), Cynodon cf Cynodon dactylon (6.257), Eragrostis tremula (5.939), Sporobolus pyramidalis (5.583), Agrostis rupestris (5.335), Axonopus compressus (4.991), and Digitaria sp. (4.544). These results show that these grass taxa have potential for sporting use in lawn mixtures at different levels of sports disciplines. 展开更多
关键词 Sports Lawn Characteristics Sports Index Natural Sports Lawn Local Grass taxa Resistance to Trampling and Pulling
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NEW TAXA OF EUGLENOPHYTA FROM CHINA
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作者 施之新 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 1995年第4期348-353,共6页
Eight new taxa of Euglenophyta are described in this paper. They were collected from severalprovinces in China and respectively named Euglena allorgei var. exsulcata. E pisciformis var. globosa, E.tortiliS, Lepocincli... Eight new taxa of Euglenophyta are described in this paper. They were collected from severalprovinces in China and respectively named Euglena allorgei var. exsulcata. E pisciformis var. globosa, E.tortiliS, Lepocinclis glabra var. papillata, Phacus pisiformis, Ph.strombuliformis,Ph.trimarginatus var.truncatus and Astasia angusta. 展开更多
关键词 NEW taxa EUGLENOPHYTA EUGLENA Lepocinclis Phacus Astasia
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Systematics, species diversity and new taxa of mesozooplankton in the West Pacific Ocean and its marginal seas: a brief compilation of monographs in China
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作者 WANG Yu HUANG Zongguo +9 位作者 WANG Chunguang HUANG Jiangshiou LIU Zhensheng XU Zhenzu HUANG Jiaqi CHEN Ruixiang LIAN Guangshan DAI Yanyu LIN Jinghong LIN Mao 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2018年第10期229-242,共14页
Research and protection of biodiversity is generally started with species recognizing guided by achievement of cataloguing and illustration obtained through in situ investigation data accumulation and literature learn... Research and protection of biodiversity is generally started with species recognizing guided by achievement of cataloguing and illustration obtained through in situ investigation data accumulation and literature learning. Facing the shortage of taxonomist, it is getting harder to maintain important specimen collections and handle loans. The West Pacific Ocean has been operating as a center of the origin of global marine biodiversity result of the richest species diversity of marine taxa found in these waters. The present work is a compilation and summary of systematics, species diversity and new taxa of mesozooplankton major group known in West Pacific Ocean and its marginal seas (0°-45°N, 105°-152°E) according to 6 203 mesozooplankton samples acquired from 701 stations during 1965 and 2008. A total of 2 657 species belonging to 686 genera and 206 families of 10 mesozooplankton groups have been found through taxonomic identification and document consulting. In details, 697 species from 251 genera of 99 families belong to the Medusozoa, 59 species from 22 genera of 12 families to the Ctenophora, 85 species from 36 genera of 14 families to the pelagic Mollusca (Pteropoda and Heteropoda), 416 species from 91 genera of 8 families to the Ostracoda, 908 species from 156 genera of 51 families to the Copepoda, 202 species from 60 genera of 4 families to the Mysidacea, 56 species from 8 genera of 2 families to the Euphausiacea, 105 species from 23 genera of 8 families to the Decapoda, 48 species from 13 genera of 5 families to the Chaetognatha and 81 species from 26 genera of 5 families to the Tunicata. The dominant species of each group are enumerated. Moreover, 2 new species of Medusozoa, Tubulariidae, Ectopleura, 1 new species of Medusozoa, Protiaridae, Halitiarella, 1 new genus and 1 new species of Medusozoa, Corymorphidae are established. An amount of 806 species are expanded with an increase of 43.5% on the basis of 1 852 species recorded before 2008, including 1.4% increase from tropical sea around equator, 4.0% from the frigid water in high latitude region, and 3.0% of bathypelagic-associated waters. The authors also summarized future prospects into five major areas in marine mesozooplankton research in China. Such information of qualitative phyletic classification could be of high relevance to studies on biodiversity and biogeography of marine mesozooplankton, especially for monographs contributed to make an overall and systematic conclusion on the species of marine life in China after 2008. 展开更多
关键词 marine mesozooplankton SYSTEMATICS species diversity new taxa COMPILATION the West Pacific Ocean marginal sea
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Stochastic community assembly of abundant taxa maintains the relationship of soil biodiversity-multifunctionality under mercury stress
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作者 Shuai Du Xin-Qi Li +6 位作者 Li Bi Dong Zhu Hang-Wei Hu Xiuli Hao Jiao Feng Qiaoyun Huang Yu-Rong Liu 《Soil Ecology Letters》 CSCD 2024年第2期163-177,共15页
Soil abundant taxa diversity positively related to multifunctionality under Hg stress.•Microbial network complexity of soil abundant taxa supported the strength of SBF.•Stochastic assembly of soil abundant subcommunit... Soil abundant taxa diversity positively related to multifunctionality under Hg stress.•Microbial network complexity of soil abundant taxa supported the strength of SBF.•Stochastic assembly of soil abundant subcommunity supported the strength of SBF.•Stochastic ratio was the most important predictor for the strength of SBF.It is known that soil microbial communities are intricately linked to multiple ecosystem functions and can maintain the relationship between soil biodiversity and multifunctionality(SBF)under environmental stresses.However,the relative contributions and driving forces of abundant and rare taxa within the communities in maintaining soil biodiversity-multifunctionality relationship under pollution stresses are still unclear.Here,we conducted microcosm experiments to estimate the importance of soil abundant and rare taxa in predicting these relationships under heavy metal mercury(Hg)stress in paired paddy and upland fields.The results revealed that the diversity of abundant taxa,rather than rare taxa,was positively related to multifunctionality,with the abundant subcommunity tending to maintain a larger proportion of soil functions including chitin degradation,protein degradation,and phosphorus mineralization.Soil multitrophic network complexity consisting of abundant species showed positive correlations with biodiversity and multifunctionality,and supported the strength of SBF within a network complexity range.Stochastic assembly processes of the abundant subcommunity were positively correlated with the strength of SBF,although stochastic processes decreased the biodiversity and the multifunctionality,respectively.After simultaneously accounting for multiple factors on the strength of SBF,we found that the stochastic community assembly ratio of abundant taxa was the most important predictor for SBF strength under Hg stress.Our results highlight the importance of abundant taxa in supporting soil multifunctionality,and elucidate the linkages between community assembly,network complexity and SBF relationship under environmental stresses. 展开更多
关键词 abundant taxa biodiversity-multifunctionality relationship community assembly network complexity environmental stresses
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酱香型白酒第六轮次堆积发酵醅堆微生物类群与感官品质的相关性分析
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作者 王玉荣 马佳佳 +3 位作者 田龙新 刘菊珍 周加平 郭壮 《现代食品科技》 CAS 北大核心 2024年第9期107-117,共11页
该研究采用高通量测序技术对酱香型白酒第六轮次堆积发酵醅堆不同位置酒醅的微生物类群进行了解析,继而使用常规和仿生检测对其品质进行了评价,最后将微生物与感官品质间进行了关联性分析。理化结果表明,醅堆不同位置酒醅中酸度、酒精... 该研究采用高通量测序技术对酱香型白酒第六轮次堆积发酵醅堆不同位置酒醅的微生物类群进行了解析,继而使用常规和仿生检测对其品质进行了评价,最后将微生物与感官品质间进行了关联性分析。理化结果表明,醅堆不同位置酒醅中酸度、酒精度和淀粉含量差异显著(P<0.05)。感官结果表明,整个醅堆挥发性风味物质以有机硫化物和萜类物质为主,滋味变化主要体现在酸味上。MiSeq结果表明,醅堆中微生物类群结构存在明显差异,其主要细菌属为高温放线菌属(Thermoactinomyces,26.46%)、克罗彭斯特菌属(Kroppenstedtia,23.42%)和乳酸杆菌属(Lactobacillus,19.05%)等,主要真菌属为酵母属(Saccharomyces,6.15%)、嗜热子囊菌属(Thermoascus,4.85%)和嗜热真菌属(Thermomyces,2.05%)等,相互菌属之间存在着密切的演替作用。相关性结果表明,醅堆不同位置酒醅中微生物与风味指标相关性不显著(P>0.05),与滋味指标相关性显著(P<0.05),且微生物代谢与酒醅中酸味的形成密切相关。由此可见,第六轮次堆积发酵醅堆不同位置酒醅的理化指标和微生物存在明显差异,且醅堆中微生物对酒醅品质的影响更多体现在滋味品质上。 展开更多
关键词 酱香型白酒 堆积发酵 高通量测序 微生物类群 相关性
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Kuedinskie Kluchiki, a Unique Middle Permian Biota Locality as a Key-point for Reconstruction of Late Paleozoic Terrestrial Ecosystems of the Urals, Russia
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作者 Serge V.NAUGOLNYKH 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2024年第4期850-866,共17页
Field work focused on the Kuedinskie Kluchiki locality, Perm region, Urals, Russia, which contains a rich assemblage of diverse fossil organisms including higher plants(equisetophytes, pteridosperms, ginkgophytes, con... Field work focused on the Kuedinskie Kluchiki locality, Perm region, Urals, Russia, which contains a rich assemblage of diverse fossil organisms including higher plants(equisetophytes, pteridosperms, ginkgophytes, conifers, vojnovskyans) represented by stems, leaves and reproductive organs;invertebrates(mollusks, arthropods), and tetrapods(temnospondyl amphibians, seymouriamorphs, cotylosaurs, synapsids, diapsids), as well as bony fishes. General characteristics of the taxonomical composition of the Kuedinskie Kluchiki locality are given. A new peltaspermalean pteridosperm taxon, Compsopteris longipinnata sp. nov., and a voltzialean conifer Archaeovoltzia kuedensis sp. nov. are described. General considerations on the paleoecologic and paleogeographic conditions of the Kuedinskie Kluchiki locality origin are given. 展开更多
关键词 PALEOBOTANY PALEOECOLOGY taxonomy Lagerst?tten new taxa peltasperms CONIFERS Wordian Cis-Urals
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Updated roles of the gut microbiota in exploring shrimp etiology, polymicrobial pathogens, and disease incidence
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作者 Jin-Bo Xiong Hao-Nan Sha Jiong Chen 《Zoological Research》 SCIE CSCD 2024年第4期910-923,共14页
Litopenaeus vannamei is the most extensively cultured shrimp species globally,recognized for its scale,production,and economic value.However,its aquaculture is plagued by frequent disease outbreaks,resulting in rapid ... Litopenaeus vannamei is the most extensively cultured shrimp species globally,recognized for its scale,production,and economic value.However,its aquaculture is plagued by frequent disease outbreaks,resulting in rapid and massive mortality.etiological research often lags behind the emergence of new diseases,leaving the causal agents of some shrimp diseases unidentified and leading to nomenclature based on symptomatic presentations,especially in cases involving co-and polymicrobial pathogens.Comprehensive data on shrimp disease statuses remain limited.In this review,we summarize current knowledge on shrimp diseases and their effects on the gut microbiome.Furthermore,we also propose a workflow integrating primary colonizers,“driver”taxa in gut networks from healthy to diseased states,disease-discriminatory taxa,and virulence genes to identify potential polymicrobial pathogens.We examine both abiotic and biotic factors(e.g.,external and internal sources and specific-disease effects)that influence shrimp gut microbiota,with an emphasis on the“holobiome”concept and common features of gut microbiota response to diverse diseases.After excluding the effects of confounding factors,we provide a diagnosis model for quantitatively predicting shrimp disease incidence using disease common-discriminatory taxa,irrespective of the causal agents.Due to the conservation of functional genes used in designing specific primers,we propose a practical strategy applying qPCR-assayed abundances of disease common-discriminatory functional genes.This review updates the roles of the gut microbiota in exploring shrimp etiology,polymicrobial pathogens,and disease incidence,offering a refined perspective for advancing shrimp aquaculture health management. 展开更多
关键词 Shrimp disease Gut microbiota Polymicrobial pathogens Diagnosis model Disease common-discriminatory taxa Disease prediction
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支持新质生产力发展的财税政策研究
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作者 张明喜 《财政科学》 CSSCI 2024年第6期18-26,共9页
通过财税政策培育新质生产力是实现高水平科技自立自强和高质量发展的重要命题。在梳理相关研究文献基础上,基于技术—经济—治理范式,从微观、中观和宏观不同视角出发,对财税政策支持新质生产力发展的逻辑进行解构。近年来,我国财税政... 通过财税政策培育新质生产力是实现高水平科技自立自强和高质量发展的重要命题。在梳理相关研究文献基础上,基于技术—经济—治理范式,从微观、中观和宏观不同视角出发,对财税政策支持新质生产力发展的逻辑进行解构。近年来,我国财税政策支持新质生产力发展取得积极进展。与此同时,适应新质生产力发展要求的财税政策面临着一系列挑战。基于理论分析和实践需求,提出支持新质生产力发展的财税政策的总体思路,并从推动科技创新、提升产业创新能力、绿色低碳发展、推进体制机制创新和优化人才工作体制机制等方面提出政策建议。 展开更多
关键词 新质生产力 逻辑解构 创新经济学 财税政策
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贵州苦苣苔科植物新种资料(8)
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作者 李飒 汤升虎 《贵州科学》 2024年第1期17-21,共5页
贵州地处中国西南,苦苣苔科植物物种丰富,近年来有若干个新种在贵州被发现,并被合格发表。为了让研究人员、学生更好地理解贵州植物区系,现逐步将这些新类群进行介绍。本篇收录了德江石蝴蝶Petrocosmea dejiangensis Sheng H.Tang&J... 贵州地处中国西南,苦苣苔科植物物种丰富,近年来有若干个新种在贵州被发现,并被合格发表。为了让研究人员、学生更好地理解贵州植物区系,现逐步将这些新类群进行介绍。本篇收录了德江石蝴蝶Petrocosmea dejiangensis Sheng H.Tang&Jian Xu、盘州石蝴蝶Petrocosmea panzhouensis Sheng H.Tang&T.Peng、开阳半蒴苣苔Hemiboea kaiyangensis T.Peng&S.Z.He以及黔渝马铃苣苔Oreocharis qianyuensis Lei Cai,J.W.Yang&Q.Zhang。 展开更多
关键词 新分类群 石蝴蝶属 半蒴苣苔属 马铃苣苔属 贵州植物区系
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梨树枝条粉碎覆盖对梨园土壤养分和微生物组的影响 被引量:1
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作者 杨天杰 王孝芳 +7 位作者 胡红菊 杨健 藕继旺 王晓祥 郝宝锋 王中华 董彩霞 徐阳春 《土壤学报》 CAS CSCD 北大核心 2024年第2期549-561,共13页
梨树整形修剪枝条是一类重要的生物质资源,将其粉碎后覆盖于树下土壤是资源化利用的有效途径。为了科学评估梨树枝条粉碎覆盖对土壤理化性状及微生物群落结构的影响,采集了贵州毕节、河北昌黎、黑龙江哈尔滨、湖北枝江和河南许昌5个不... 梨树整形修剪枝条是一类重要的生物质资源,将其粉碎后覆盖于树下土壤是资源化利用的有效途径。为了科学评估梨树枝条粉碎覆盖对土壤理化性状及微生物群落结构的影响,采集了贵州毕节、河北昌黎、黑龙江哈尔滨、湖北枝江和河南许昌5个不同纬度梨典型种植区梨园枝条覆盖1~2年后的土壤样品,检测了土壤基础理化特性及土壤细菌、真菌和原生生物群落。结果发现,与不覆盖枝条的对照相比,总体而言覆盖能有效提高土壤有机质和有效磷含量,土壤细菌、真菌和原生生物群落结构也发生了显著变化,但对群落多样性无显著影响;覆盖处理显著富集了土壤细菌中的变形菌门、酸杆菌门和真菌中的担子菌门以及原生生物中的孔虫界和囊泡虫类等类群的相对丰度。进一步利用随机森林模型挖掘了不同采样点枝条覆盖后的关键物种,模型预测准确率高达94.44%,关键物种以细菌为主,包括Acidibacter、Xanthobacteraceae、Nitrosomonadaceae等与有机残体降解有关的微生物,还发现土壤有效磷含量是土壤微生物群落结构差异的主要驱动因子。相关研究结果初步评估了梨树枝条覆盖对土壤养分和微生物组的影响,挖掘了潜在的关键微生物。 展开更多
关键词 梨修剪枝条 覆盖 土壤理化性状 微生物组 关键物种
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长期有机肥和化肥配施对黑土细菌群落特征和大豆产量的影响 被引量:2
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作者 方海瑞 刘俊杰 +5 位作者 陈雪丽 姜宇 刘株秀 顾海东 万书明 肖洋 《中国生态农业学报(中英文)》 CAS CSCD 北大核心 2024年第5期804-815,共12页
有机物料还田是提高土壤微生物多样性和提升土壤质量的主要措施,但目前有机物料还田对黑土关键土壤微生物类群的影响及其与大豆产量间的关系尚未充分明确。本研究基于东北地区44年长期黑土定位试验,研究不同施肥措施与土壤理化性质、细... 有机物料还田是提高土壤微生物多样性和提升土壤质量的主要措施,但目前有机物料还田对黑土关键土壤微生物类群的影响及其与大豆产量间的关系尚未充分明确。本研究基于东北地区44年长期黑土定位试验,研究不同施肥措施与土壤理化性质、细菌群落和大豆产能间的关联特征。结果表明:与无肥(NoF)对比,单施有机肥(M)和化肥配施有机肥(CFM)处理显著增加了土壤养分含量、微生物量碳和微生物量氮含量。化肥配施秸秆(CFS)处理的土壤细菌丰富度和多样性低于单施秸秆(S),而化肥配施有机肥(CFM)处理的土壤细菌丰富度和多样性高于单施有机肥处理(M)。相比于NoF,施肥导致富营养型细菌类群(r策略)与寡营养型细菌类群(K策略)的比值增加。长期秸秆还田(S和CFS)和施用化肥(CF)增加了细菌网络复杂性,而长期添加有机肥(M和CFM)降低了网络复杂性。结构方程模型表明土壤铵态氮、碳氮比、细菌网络结构复杂性和关键类群[硝化螺旋菌属(Nitrospira)与芽单胞菌属(Gemmatimonas)]丰度与大豆产量呈显著正相关关系。有机肥配施化肥(CFM)有利于提高关键功能微生物类群丰度、群落多样性和大豆产量。 展开更多
关键词 黑土区 施肥措施 土壤理化性状 细菌关键类群 大豆产量
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生物多样性在低碳农业中的应用现状及展望 被引量:1
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作者 陈俊斌 胡广 《农学学报》 2024年第4期42-51,共10页
在中国推进生态文明建设的背景下,农业固碳增效被写入“双碳目标”,低碳农业随之兴起。生物多样性是生态系统功能与服务的基础,在农业低碳化过程中发挥了至关重要的作用,保护农田生物多样性,充分发挥其生态作用是实现农业减排固碳的重... 在中国推进生态文明建设的背景下,农业固碳增效被写入“双碳目标”,低碳农业随之兴起。生物多样性是生态系统功能与服务的基础,在农业低碳化过程中发挥了至关重要的作用,保护农田生物多样性,充分发挥其生态作用是实现农业减排固碳的重要路径。收集“Web of Science”数据库中2010-2022年的文献,利用CiteSpace文献计量软件的关键词共现以及聚类分析,对近年来国际低碳农业中生物多样性的研究动态、研究热点进行分析,对生物多样性的功能进行归纳总结。分析结果表明,生物多样性的保护与管理是目前低碳农业生物多样性的研究热点,而生物防治是生物多样性实现农业低碳化的主要途径,通过对不同生物类群多样性的生态价值进行梳理,发现生物多样性可以增加农业生产过程中的生态与经济价值。未来的研究可从生态系统和景观的尺度,探索基于生物多样性的多功能农业发展模式,从而更好地助力双碳目标的顺利实现。 展开更多
关键词 农业低碳化 生物多样性 增产 品质提升 病虫害控制 传粉 生物类群
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基于稳定性同位素核酸探针技术的同化利用玉米秸秆碳微生物群落演替研究
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作者 陈霄龙 郭腾飞 +6 位作者 张倩 岳克 张珂珂 宋晓 丁世杰 张水清 黄绍敏 《植物营养与肥料学报》 CAS CSCD 北大核心 2024年第3期430-440,共11页
[目的]研究秸秆分解过程中同化利用秸秆碳源的微生物生态演替过程,明确参与秸秆分解的主要微生物类群,为秸秆高效利用提供科学依据。[方法]采用^(13)C标记高丰度玉米秸秆。微宇宙室内培养试验的供试土壤为华北平原石灰性潮土。共设置3... [目的]研究秸秆分解过程中同化利用秸秆碳源的微生物生态演替过程,明确参与秸秆分解的主要微生物类群,为秸秆高效利用提供科学依据。[方法]采用^(13)C标记高丰度玉米秸秆。微宇宙室内培养试验的供试土壤为华北平原石灰性潮土。共设置3个处理:不添加秸秆(soil)、添加自然丰度秸秆(soil+^(12)C-straw)和添加13C标记秸秆(soil+^(13)C-straw)。培养30天时,结合同位素示踪与高通量测序等技术,测定秸秆添加对土壤有机碳的激发效应,分析利用秸秆碳源的细菌、真菌群落结构及其与土壤胞外酶活性的关系。[结果](1)秸秆添加显著提高了土壤CO^(2)排放,添加秸秆对土壤有机碳的激发效应在培养第1天达到峰值,土壤和秸秆CO^(2)排放比例均在培养第3天达到峰值。(2)整个培养时期共发现参与秸秆碳同化利用的细菌微生物操作分类单元(operational taxonomic units,OTU_(s))238个,真菌OTU_(s)24个。细菌OTU_(s)主要属于细菌变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、拟杆菌门(Bacteroidetes)与绿弯菌门(Chloroflexi),真菌OTU_(s)主要为子囊菌门中的粪壳菌纲(Sordariomycetes)。(3)秸秆分解不同时期的微生物群落结构存在显著差异,在整个培养时期,以热单胞菌属(Thermomonas)与溶杆菌属(Lysobacter)为代表的7个细菌属快速响应秸秆添加,以假黄单胞菌属(Pseudoxanthomonas)与土生单胞菌属(Terrimonas)为代表的6个细菌属延迟响应;两个真菌属枝鼻菌属(Cladorrhinum)与葡萄穗霉属(Stachybotrys)表现为对秸秆添加的快速响应,新赤壳属(Neocosmospora)与unclassified_f_Halosphaeriaceae为延迟响应。(4)曼特尔分析表明,细菌热单胞菌属(Thermomonas)、溶杆菌属(Lysobacter)、绿弯菌门(Chloroflexi)以及真菌裂壳菌属(Schizothecium)与碳氮转化相关土壤胞外酶活性呈显著正相关关系。[结论]外源秸秆添加对土壤有机碳产生正激发效应,显著增加了土壤CO^(2)排放。同化秸秆碳源的微生物随培养时间延长发生群落演替,细菌的热单胞菌属(Thermomonas)、溶杆菌属(Lysobacter)、绿弯菌门(Chloroflexi)以及真菌的裂壳菌属(Schizothecium)在玉米秸秆分解过程中具有重要作用,可为秸秆高效腐熟菌剂的研发提供依据。 展开更多
关键词 ^(13)C玉米秸秆 DNA稳定同位素核酸探针 微生物类群 群落演替
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隆中对酒业中温大曲真菌类群与理化特性相关性研究
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作者 贺子豪 马佳佳 +4 位作者 王俊麟 刘博文 张莹 郭壮 王玉荣 《食品工业科技》 CAS 北大核心 2024年第2期136-142,共7页
为剖析湖北襄阳地区中温大曲真菌类群与其理化特性之间的关联性,本研究采用高通量测序与纯培养技术对湖北隆中对酒业中温大曲的真菌组成进行了解析,并结合理化与风味指标对大曲品质进行了评价,继而揭示了真菌类群对其品质的影响。结果表... 为剖析湖北襄阳地区中温大曲真菌类群与其理化特性之间的关联性,本研究采用高通量测序与纯培养技术对湖北隆中对酒业中温大曲的真菌组成进行了解析,并结合理化与风味指标对大曲品质进行了评价,继而揭示了真菌类群对其品质的影响。结果表明,大曲样本的各理化指标均符合QB/T 4259-2011《浓香大曲》要求,且样品间酯化力和糖化力差异较大(P<0.05)。嗜热真菌属(Thermomyces)、根霉属(Rhizopus)、双足囊菌属(Dipodascus)、曲霉属(Aspergillus)、毕赤酵母属(Pichia)、丝孢毕赤酵母属(Hyphopichia)和青霉属(Penicillium)为其主要真菌属。分离的25株酵母菌被鉴定为扣囊复膜孢酵母(Saccharomycopsis fibuligera)和异常威克汉姆酵母(Wickerhamomyces anomalus)。相关性结果表明Hyphopichia对芳香型化合物的形成具有积极作用(P<0.05)。由此可见,湖北隆中对酒业中温大曲蕴含多种真菌有待发掘,且部分真菌代谢有利于有机硫化物和芳香类化合物的形成。 展开更多
关键词 中温大曲 真菌类群 理化特性 风味品质 关联分析
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兰陵酒业高温大曲细菌类群与理化指标关联性分析
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作者 张凯 黄韦菁 +4 位作者 向绪军 管桂坤 刘宇 郭壮 王玉荣 《食品工业科技》 CAS 北大核心 2024年第19期124-132,共9页
为分析山东临沂地区高温大曲细菌类群及其理化特性,本研究采用Illumina MiSeq高通量测序技术和纯培养技术对兰陵酒业高温大曲中细菌类群进行了解析,通过常规检测方法对其理化指标进行了测定,探究大曲中细菌类群与理化指标之间的关联性... 为分析山东临沂地区高温大曲细菌类群及其理化特性,本研究采用Illumina MiSeq高通量测序技术和纯培养技术对兰陵酒业高温大曲中细菌类群进行了解析,通过常规检测方法对其理化指标进行了测定,探究大曲中细菌类群与理化指标之间的关联性。结果表明,大曲中细菌属主要为克罗彭斯特德菌属(Kroppenstedtia)、高温放线菌属(Thermoactinomyces)、糖多孢菌属(Saccharopolyspora)、葡萄球菌属(Staphylococcus)、红球菌属(Rhodococcus)、魏斯氏菌属(Weissella)和芽孢杆菌属(Bacillus)等。Bacillus subtilis、Bacillus licheniformis和Bacillus coagulans为可培养优势细菌分离株。15份高温大曲间糖化力、酯化力、酒化力和液化力差异较大。Kroppenstedtia、Staphylococcus、Leucobacter、Rhodococcus和Weissella与酯化力、发酵力、酒化力、酯化力和液化力间呈现显著相关性(P<0.05)。由此可见,兰陵酒业高温大曲中存在大量核心细菌类群,且各细菌属与理化指标间存在明显相关性,进一步为该地区高温大曲品质的改善与提升提供参考。 展开更多
关键词 高温大曲 高通量测序技术 细菌类群 理化指标 相关性
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凡纳滨对虾循环水养殖系统生物膜反硝化菌研究
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作者 李雅媛 张旺 +3 位作者 刘兴国 肖述文 江海鑫 陆诗敏 《水产科学》 CAS CSCD 北大核心 2024年第5期775-783,共9页
硝态氮去除对于养殖尾水再利用及达标排放具有重要意义。为探明凡纳滨对虾循环水养殖系统中移动床生物反应器的反硝化潜力和氮代谢通路特征,对其表面生物膜菌群结构和反硝化效率进行分析。Illumina测序结果显示:所有样本优势菌门均为变... 硝态氮去除对于养殖尾水再利用及达标排放具有重要意义。为探明凡纳滨对虾循环水养殖系统中移动床生物反应器的反硝化潜力和氮代谢通路特征,对其表面生物膜菌群结构和反硝化效率进行分析。Illumina测序结果显示:所有样本优势菌门均为变形菌门(20.31%~25.36%)和拟杆菌门(14.42%~24.28%);在属水平上,红球菌属(0.28%~0.97%)、副球菌属(0.50%~0.99%)和假单胞菌属(0.15%~0.30%)等好氧反硝化菌主要负责硝态氮还原。实时荧光定量PCR结果显示,生物膜中反硝化功能基因nirK基因丰度高于nirS基因和nosZ基因,nirK型反硝化微生物是主要的反硝化微生物类群。原核生物功能注释结果表明,生物膜中存在活跃的异养反硝化代谢途径,可能受制于有限碳源,反硝化菌之间,及其和生物膜优势菌属之间以竞争关系为主。厌氧脱氮模拟试验表明,在不添加碳源条件下,填料生物膜可将对虾养殖水体硝态氮去除98.07%。基于上述氮代谢通路特征,本试验进一步提出短程反硝化-厌氧氨氧化对虾养殖废水硝态氮去除优化策略。此结果为深入理解循环水养殖系统氮素代谢通路及未来管理、利用填料生物膜内源性碳源和潜在反硝化功能提供理论依据。 展开更多
关键词 nirS基因 nirK基因 nosZ基因 移动床生物反应器 原核生物功能注释
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Quantitative relationships between vegetation and several pollen taxa in surface soil from North China 被引量:25
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作者 LI Yiyin, ZHANG Xinshi & ZHOU Guangsheng1. Department of Urbon and Environmental Sciences, Peking University, Beijing 100871, China 2. Laboratory of Quantitative Vegetation Ecology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 3. School of Urban and Environmental Sciences, Northeast Normal University, Changchun 130024, China Correspondence should be addressed to Li Yiyin 《Chinese Science Bulletin》 SCIE EI CAS 2000年第16期1519-1523,共5页
According to the vegetation investigation and pollen analysis of surface samples sampled along a precipitation gradient of the Northeast China Transect (NECT), several pollen taxa, including Pinus, Betula, Quercus, Ti... According to the vegetation investigation and pollen analysis of surface samples sampled along a precipitation gradient of the Northeast China Transect (NECT), several pollen taxa, including Pinus, Betula, Quercus, Tilia, Acer, Ulmus, Artemisia, Chenopodiaceae, Gramineae and Cyperaceae, were chosen to make the regression and correlation analyses. The results indicated that there exists a close relationship between vegetation and pollen taxa in surface samples. The regression parameters for ten taxa in the forests in the eastern part of NECT were different from those in the steppes in the western part. Pinus, Betula, Artemisia and Chenopodiaceae, which have large slope and y-intercept terms, were over-representative taxa. Acer, Gramineae and Cyperaceae, which have small slope and y-intercept terms, were under-representative taxa. Quercus, Tilia and Ulmus whose slope terms have negative correlation with y-intercept terms were equi-representative taxa. The pollen taxa with large slope or large y-intercept 展开更多
关键词 POLLEN taxa in surface soil VEGETATION regression parameters (slope TERM and y-intercept term) representation Northeast China TRANSECT (NECT).
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盐酸四环素长期暴露对序批式反应器性能、微生物酶活性及微生物群落的影响
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作者 齐维毅 王倩芝 +6 位作者 楚光玉 陈文正 张雨乔 刘甲滕 高畅 鲁帅领 高孟春 《中国海洋大学学报(自然科学版)》 CAS CSCD 北大核心 2024年第3期88-98,共11页
本文研究了0.5 mg/L盐酸四环素(TC)长期暴露对序批式反应器(SBR)性能、脱氮酶活性、抗氧化酶和微生物群落的影响。结果表明,SBR中有机物去除能力和氨氧化能力随运行时间的延长而降低,在反应器运行第70天时,COD去除率从97.10%降低至70.33... 本文研究了0.5 mg/L盐酸四环素(TC)长期暴露对序批式反应器(SBR)性能、脱氮酶活性、抗氧化酶和微生物群落的影响。结果表明,SBR中有机物去除能力和氨氧化能力随运行时间的延长而降低,在反应器运行第70天时,COD去除率从97.10%降低至70.33%,NH_(4)^(+)-N去除率从99.24%降低至67.44%,出水NO_(3)^(-)-N逐渐降低,SBR内没有出现NO_(2)^(-)-N积累。TC长期暴露降低了氨单加氧酶(AMO)、亚硝酸盐氧化酶(NOR)、硝酸盐还原酶(NR)和亚硝酸盐还原酶(NIR)等脱氮酶活性,增加了活性氧(ROS)的相对产生量、乳酸脱氢酶(LDH)相对释放量和部分抗氧化酶的相对活性。进水中加入TC后,微生物的丰富度、多样性和均匀性均降低,硝化菌属(Nitrospira)和反硝化菌属(Zoogloea、Thauera、Dechloromonas、Dokdonella、Hyphomicrobium和Pseudomonas)的相对丰度发生变化,微生物群落和关键微生物具有显著性差异,从而影响了SBR的脱氮性能。 展开更多
关键词 盐酸四环素 微生物酶活性 微生物群落 LEfSe分析 关键微生物
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