Escherichia coli O157:H7 is one of the major foodborne pathogenic bacterial that cause infectious diseases in humans.The previous found that a combination of kojic acid and tea polyphenols exhibited better activity ag...Escherichia coli O157:H7 is one of the major foodborne pathogenic bacterial that cause infectious diseases in humans.The previous found that a combination of kojic acid and tea polyphenols exhibited better activity against E.coli O157:H7 than using either alone.This study aimed to explore responses underlying the antibacterial mechanisms of kojic acid and tea polyphenols from the gene level.The functional enrichment analysis by comparing kojic acid and tea polyphenols individually or synergistically against E.coli O157:H7 found that acid resistance systems in kojic acid were activated,and the cell membrane and genomic DNA were destructed in the cells,resulting in“oxygen starvation”.The oxidative stress response triggered by tea polyphenols inhibited both sulfur uptake and the synthesis of ATP,which affected the bacteria's life metabolic process.Interestingly,we found that kojic acid combined with tea polyphenols hindered the uptake of iron that played an essential role in the synthesis of DNA,respiration,tricarboxylic acid cycle.The results suggested that the iron uptake pathways may represent a novel approach for kojic acid and tea polyphenols synergistically against E.coli O157:H7 and provided a theoretical basis for bacterial pathogen control in the food industry.展开更多
The interaction between host circadian rhythm and gut microbes through the gut-brain axis provides new clues for tea polyphenols to improve host health.Our present research showed that oolong tea polyphenols(OTP)impro...The interaction between host circadian rhythm and gut microbes through the gut-brain axis provides new clues for tea polyphenols to improve host health.Our present research showed that oolong tea polyphenols(OTP)improved the structural disorder of the intestinal flora caused by continuous darkness,thereby modulating the production of metabolites related to pyruvate metabolism,glycolysis/gluconeogenesis,and tryptophan metabolism to alleviate the steady-state imbalance.After fecal microbiota transplantation from the OTP group,the single-cell transcriptomic analysis revealed that OTP significantly increased the number of hypothalamus cell clusters,up-regulated the number of astrocytes and fibroblasts,and enhanced the expression of circadian rhythm genes Cry2,Per3,Bhlhe41,Nr1d1,Nr1d2,Dbp and Rorb in hypothalamic cells.Our results confirmed that OTP can actively improve the intestinal environmental state as well as internal/peripheral circadian rhythm disorders and cognitive impairment,with potential prebiotic functional characteristics to notably contribute to host health.展开更多
Membrane pollution caused by separating oily wastewater is a big challenge for membrane separation technology.Recently,plant-/mussel-inspired interface chemistry has received more and more attention.Herein,a high anti...Membrane pollution caused by separating oily wastewater is a big challenge for membrane separation technology.Recently,plant-/mussel-inspired interface chemistry has received more and more attention.Herein,a high antifouling poly(vinylidene fluoride)(PVDF)membrane,coated with tea polyphenols(TP,extracted from green tea)and 3-amino-propyl-triethoxysilane(APTES),was developed to purify oil-inwater emulsions.ATR-FTIR,XPS and SEM were used to demonstrate the evolution of surface biomimetic hybrid coatings.The performances of the developed membranes were investigated by pure water permeability and oil rejection for various surfactant-stabilized oil-in-water emulsions.The experimental results revealed that the membrane deposited with a mass ratio of 0.1/0.2 exhibited ultrahigh pure water permeability(14570 L·m^(-2)·h^(-1)·bar^(-1),1 bar=0.1 MPa)and isooctane-in-water emulsion permeability(5391 L·m^(-2)·h^(-1)·bar^(-1))with high separation efficiency(>98.9%).Even treated in harsh environment(acidic,alkaline and saline)for seven days,the membrane still maintained considerable underwater oleophobic property(148°–153°).The fabricated plant-inspired biomimetic hybrid membranes with excellent performances light a broad application prospect in the field of oily wastewater treatment.展开更多
BACKGROUND Di(2-ethylhexyl)phthalate(DEHP)is a common plasticizer known to cause liver injury.Green tea is reported to exert therapeutic effects on heavy metal exposureinduced organ damage.However,limited studies have...BACKGROUND Di(2-ethylhexyl)phthalate(DEHP)is a common plasticizer known to cause liver injury.Green tea is reported to exert therapeutic effects on heavy metal exposureinduced organ damage.However,limited studies have examined the therapeutic effects of green tea polyphenols(GTPs)on DEHP-induced liver damage.AIM To evaluate the molecular mechanism underlying the therapeutic effects of GTPs on DEHP-induced liver damage.METHODS C57BL/6J mice were divided into the following five groups:Control,model[DEHP(1500 mg/kg bodyweight)],treatment[DEHP(1500 mg/kg bodyweight)+GTP(70 mg/kg bodyweight),oil,and GTP(70 mg/kg bodyweight)]groups.After 8 wk,the liver function,blood lipid profile,and liver histopathology were examined.Differentially expressed micro RNAs(miRNAs)and mRNAs in the liver tissues were examined using high-throughput sequencing.Additionally,functional enrichment analysis and immune infiltration prediction were performed.The miRNA-mRNA regulatory axis was elucidated using the starBase database.Protein expression was evaluated using immunohistochemistry.RESULTS GTPs alleviated DHEP-induced liver dysfunction,blood lipid dysregulation,fatty liver disease,liver fibrosis,and mitochondrial and endoplasmic reticulum lesions in mice.The infiltration of macrophages,mast cells,and natural killer cells varied between the model and treatment groups.mmu-miR-141-3p(a differentially expressed miRNA),Zcchc24(a differentially expressed mRNA),and Zcchc24(a differentially expressed protein)constituted the miRNA-mRNA-protein regulatory axis involved in mediating the therapeutic effects of GTPs on DEHP-induced liver damage in mice.CONCLUSION This study demonstrated that GTPs mitigate DEHP-induced liver dysfunction,blood lipid dysregulation,fatty liver disease,and partial liver fibrosis,and regulate immune cell infiltration.Additionally,an important miRNAmRNA-protein molecular regulatory axis involved in mediating the therapeutic effects of GTPs on DEHP-induced liver damage was elucidated.展开更多
Objective:This study aimed to explore the effects of tea polyphenols(TP)on inflammation of orbital fibroblasts in Graves’ophthalmopathy(GO)and to provide new ideas for GO treatment.Methods:Primary orbital fibroblasts...Objective:This study aimed to explore the effects of tea polyphenols(TP)on inflammation of orbital fibroblasts in Graves’ophthalmopathy(GO)and to provide new ideas for GO treatment.Methods:Primary orbital fibroblasts were extracted from orbital adipose/connective tissues of patients with and without GO.Real-time quantitative PCR(RT-qPCR)was used to detect the expression of interleukin(IL)-6,IL-1β,and monocyte chemotactic protein(MCP)-1 in non-GO and GO orbital fibroblasts.The CCK-8 assay was used to determine the appropriate concentration of TP for subsequent experiments.RT-qPCR and enzyme-linked immunosorbent assay(ELISA)were performed to investigate the effects of TP on lipopolysaccharide(LPS)-induced production of inflammatory cytokines.Nuclear factor-κB(NF-κB)expression was measured using Western blotting analysis.NOD-like receptor 3(NLRP3)expression was detected using both Western blotting analysis and immunofluorescence staining.Results:The mRNA levels of IL-6,IL-1β,and MCP-1 in GO orbital fibroblasts were significantly higher than those in non-GO cells.TP treatment significantly inhibited LPS-induced production of inflammatory factors,including IL-6,IL-1β,and MCP-1.TP also inhibited the expression levels of NF-κB and NLRP3.Inflammation in the GO orbital fibroblasts was higher than that in non-GO cells.TP inhibited the production of inflammatory cytokines in GO orbital fibroblasts in vitro through the NF-κB/NLRP3 pathway.Conclusion:These findings suggest that TP may have a potential role in GO treatment.展开更多
In order to effectively control the drug-release rate of medical textiles,biodegradable polycaprolactone(PCL) and polyglycolic acid(PGA) were blended at various mass ratios to prepare composite masterbatches for medic...In order to effectively control the drug-release rate of medical textiles,biodegradable polycaprolactone(PCL) and polyglycolic acid(PGA) were blended at various mass ratios to prepare composite masterbatches for medical textiles.The surface morphology and the chemical structure of the masterbatches were analyzed.The crystallization,mass losses,strengths and drug-release rates of the composite masterbatches at different PCL/PGA mass ratios were explored.The results show that the degradation rate of the PGA carrier is obvious higher than that of the PCL carrier,and PCL,PGA and the tea polyphenol(TP) drug just physically mix without chemical reaction.During the degradation,the strength of the composite masterbatches gradually decreases.In addition,the drug-release rates of composite masterbatches at different mass ratios are different,and the more the PGA in the composite masterbatches,the faster the drug release of the composite masterbatches.The drug-release rate of the composite masterbatches can be controlled by adjusting the contents of PCL and PGA.展开更多
In recent years, the incidence of neurodegenerative diseases, mainly Alzheimer’s disease, Parkinson’s disease, vascular dementia, and cerebral ischemia, has been rising gradually, which has a serious impact on the p...In recent years, the incidence of neurodegenerative diseases, mainly Alzheimer’s disease, Parkinson’s disease, vascular dementia, and cerebral ischemia, has been rising gradually, which has a serious impact on the physiological state and quality of life of human beings in old age, and the current clinical drugs are unsatisfactory in terms of therapeutic efficacy and healing, which has made this kind of diseases become a social medical problem. Tea polyphenols are the main functional components of tea and have great potential in neuroprotection. In this paper, we review the research on tea polyphenols in neurodegenerative diseases, with the aim of providing a new entry point for the treatment of neurodegenerative diseases.展开更多
Tea polyphenols(TP)is a class of polyhydroxy compounds isolated from tea.Modern biological and medical studies have shown that TP has many pharmacological activities,such as anti-inflammatory,anti-virus,anti-oxidation...Tea polyphenols(TP)is a class of polyhydroxy compounds isolated from tea.Modern biological and medical studies have shown that TP has many pharmacological activities,such as anti-inflammatory,anti-virus,anti-oxidation,anti-tumor and anti-radiation.Furthermore,these substances can be used as a potential drug component to positively guide the occurrence and development of certain diseases.Furthermore,because of the activities of TP,such as anti-oxidation and anti-bacteria,it can be applied in food preservation,color preservation,deodorization,and treatment of food processing by-products.Based on the research progress of TP in recent years,this paper summarizes the pharmacological activities of TP and expounds on its application potential in the field of food.In order to provide a theoretical reference for the research,development and utilization of TP.展开更多
AIM To investigate protective effects and molecular mechanisms of green tea polyphenols(GTP) on nonalcoholic fatty liver disease(NAFLD) in Zucker fatty(ZF) rats.METHODS Male ZF rats were fed a high-fat diet(HFD) for 2...AIM To investigate protective effects and molecular mechanisms of green tea polyphenols(GTP) on nonalcoholic fatty liver disease(NAFLD) in Zucker fatty(ZF) rats.METHODS Male ZF rats were fed a high-fat diet(HFD) for 2 wk then treated with GTP(200 mg/kg) or saline(5 m L/kg) for 8 wk, with Zucker lean rat as their control. At the end of experiment, serum and liver tissue were collected for measurement of metabolic parameters, alanine aminotransferase(ALT) and aspartate aminotransferase(AST), inflammatory cytokines and hepatic triglyceride and liver histology. Immunoblotting was used to detect phosphorylation of AMP-activated protein kinase(AMPK) acetyl-Co A carboxylase(ACC), and sterol regulatory element-binding protein 1c(SREBP1c). RESULTS Genetically obese ZF rats on a HFD presented with metabolic features of hepatic pathological changes comparable to human with NAFLD. GTP intervention decreased weight gain(10.1%, P = 0.052) and significantly lowered visceral fat(31.0%, P < 0.01). Compared with ZF-controls, GTP treatment significantly reduced fasting serum insulin, glucose and lipids levels. Reduction in serum ALT and AST levels(both P < 0.01) were observed in GTP-treated ZF rats. GTP treatment also attenuated the elevated TNFα and IL-6 in the circulation. The increased hepatic TG accumulation and cytoplasmic lipid droplet were attenuated by GTP treatment, associated with significantly increased expression of AMPK-Thr172(P < 0.05) and phosphorylated ACC and SREBP1c(both P < 0.05), indicating diminished hepatic lipogenesis and triglycerides out flux from liver in GTP treated rats. CONCLUSION The protective effects of GTP against HFD-induced NAFLD in genetically obese ZF rats are positively correlated to reduction in hepatic lipogenesis through upregulating the AMPK pathway.展开更多
Colorectal cancer(CRC),a multifactorial disease,is usually induced and developed through complex mechanisms,including impact of diet and lifestyle,genomic abnormalities,change of signaling pathways,inflammatory respon...Colorectal cancer(CRC),a multifactorial disease,is usually induced and developed through complex mechanisms,including impact of diet and lifestyle,genomic abnormalities,change of signaling pathways,inflammatory response,oxidation stress,dysbiosis,and so on.As natural polyphenolic phytochemicals that exist primarily in tea,tea polyphenols(TPs)have been shown to have many clinical applications,especially as anticancer agents.Most animal studies and epidemiological studies have demonstrated that TPs can prevent and treat CRC.TPs can inhibit the growth and metastasis of CRC by exerting the antiinflammatory,anti-oxidative or pro-oxidative,and pro-apoptotic effects,which are achieved by modulations at multiple levels.Many experiments have demonstrated that TPs can modulate several signaling pathways in cancer cells,including the mitogen-activated protein kinase pathway,phosphatidylinositol-3 kinase/Akt pathway,Wnt/β-catenin pathway,and 67 kDa laminin receptor pathway,to inhibit proliferation and promote cell apoptosis.In addition,novel studies have also suggested that TPs can prevent the growth and metastasis of CRC by modulating the composition of gut microbiota to improve immune system and decrease inflammatory responses.Molecular pathological epidemiology,a novel multidisciplinary investigation,has made great progress on CRC,and the further molecular pathological epidemiology research should be developed in the field of TPs and CRC.This review summarizes the existing in vitro and in vivo animal and human studies and potential mechanisms to examine the effects of tea polyphenols on CRC.展开更多
Myriad evidence attests to the health-promoting benefits of tea drinking.While there are multiple factors of tea influencing the effective biological properties,tea polyphenols are the most significant and valuable co...Myriad evidence attests to the health-promoting benefits of tea drinking.While there are multiple factors of tea influencing the effective biological properties,tea polyphenols are the most significant and valuable components.The chemical characterization and physical characteristics of tea polyphenols have been comprehensively studied over the previous years.Still the emergence of new chemistry in tea,particularly the property of scavenging reactive carbonyl species(RCS)and the newly discovered flavoalkaloid compounds,has drawn increasing attention.In this review,we summarize recent findings of a new class of compounds in tea-flavonoid alkaloids(flavoalkaloids),which exist in fresh tea leaves and can be generated during the process of post-harvesting,and also postulate the formation mechanism of flavoalkaloids between catechins and theanine-derived Strecker aldehyde.Additionally,we detail the up-to-date research results of tea polyphenols regarding their ability to trap RCS and their in vivo aminated metabolites to suppress advanced glycation ends products(AGEs).We further raise questions to be addressed in the near future,including the synthetic pathways for the generation of flavoalkaloids and AGEs in fresh tea leaves before processing and the concentrations of tea polyphenols that affect their RCS scavenging capability due to their pro-oxidant nature.More intensive research is warranted to elucidate the mechanisms of action underlying the biological activity of flavoalkaloids and the pharmacological application of tea polyphenols in scavenging RCS and impeding detrimental AGEs.展开更多
Polyphenols,including phenolic acids,flavonoids,and procyanidins,are abundant in food and beverage derived from plants.Tea(Camellia sinensis)is particularly rich in polyphenols(e.g.,catechins,theaflavins,thearubigins,...Polyphenols,including phenolic acids,flavonoids,and procyanidins,are abundant in food and beverage derived from plants.Tea(Camellia sinensis)is particularly rich in polyphenols(e.g.,catechins,theaflavins,thearubigins,gallic acid,and flavonols),which are thought to contribute to the health benefits of tea.High intake of tea polyphenols has been described to prevent and/or attenuate a variety of chronic pathological conditions like cardiovascular diseases,neurodegenerative diseases,diabetes,and cancer.This review focuses on established antioxidant and anti-inflammatory properties of tea polyphenols and underlying mechanisms of their involvement in inflammatory bowel diseases(IBD).Tea polyphenols act as efficient antioxidants by inducing an endogenous antioxidant defense system and maintaining intracellular redox homeostasis.Tea polyphenols also regulate signaling pathways such as nuclear factor-κB,activator protein 1,signal transducer and activator of transcriptions,and nuclear factor E2-related factor 2,which are associated with IBD development.Accumulating pieces of evidence have indicated that tea polyphenols enhance epithelial barrier function and improve gut microbial dysbiosis,contributing to the management of inflammatory colitis.Therefore,this study suggests that supplementation of tea polyphenols could prevent inflammatory conditions and improve the outcome of patients with IBD.展开更多
Tea polyphenols(TPPs)have attracted significant research interest due to their health benefits.However,TPPs are sensitive to certain environmental and gastrointestinal conditions and their oral bioavailability was fou...Tea polyphenols(TPPs)have attracted significant research interest due to their health benefits.However,TPPs are sensitive to certain environmental and gastrointestinal conditions and their oral bioavailability was found to be very low.Delivery systems made of food-grade materials have been reported to improve the shelf-life,bioavailability and bioefficacy of TPPs.This review discusses the chemistry of TPPs;the setbacks of TPPs for application;and the strategies to counteract application limitations by rationally designing delivery systems.An overview of different formulations used to encapsulate TPPs is provided in this study,such as emulsion-based systems(liposome,nanoemulsion,double emulsion,and Pickering emulsion)and nano/microparticles-based systems(protein-based,carbohydrate-based,and bi-polymer based).In addition,the stability,bioavailability and bioactivities of encapsulated TPPs are evaluated by various in vitro and in vivo models.The current findings provide scientific insights in encapsulation approaches for the delivery of TPPs,which can be of great value to TPPs-fortified food products.Further explorations are needed for the encapsulated TPPs in terms of their applications in the real food industry as well as their biological fate and functional pathways in vivo.展开更多
The metabolic syndrome(MS)has become one of the main problems in public health.Tea polyphenols(TPs),the main bioactive components of tea,has been claimed to have the potential to regulate metabolism and effectively pr...The metabolic syndrome(MS)has become one of the main problems in public health.Tea polyphenols(TPs),the main bioactive components of tea,has been claimed to have the potential to regulate metabolism and effectively prevent or mitigate the MS.However,many studies into the effects of TPs on MS have provided conflicting findings and the underlying mechanism has been elusive.The predominant TPs in unfermentedand and fermented tea are catechins and oxidized polyphenols(theaflavins and thearubigins),both of which have low bioavailability and reach the colon where most gut microbes inhabit.Gut microbiota has been demonstrated to be tightly associated with host metabolism.The interactions between TPs and gut microbiota will lead to the alterations of gut microbiota composition and the production of metabolites including short chain fatty acids,bile acids,amino acids and TPs derived metabolites,accordingly exerting their biological effects both locally and systemically.This review highlighted the contribution of metabolites and specific gut bacteria in the process of TPs intervention on the MS and further discuss how TPs impact the MS via gut microbiota from the viewpoint of gut organ/tissue axis.展开更多
This paper elaborated the chemical components,biological metabolism,and progress in the field of drug development of green tea polyphenols,mainly in the prevention and treatment of cancer,neurodegenerative diseases,an...This paper elaborated the chemical components,biological metabolism,and progress in the field of drug development of green tea polyphenols,mainly in the prevention and treatment of cancer,neurodegenerative diseases,and diabetes.The potential anti-tumor activity of tea polyphenols can be achieved through intervening in various stages of tumor generation,development,and metastasis.However,the development of tea polyphenols as a therapeutic drug still faces many challenges,such as low bioavailability.Nanoparticle-based drug delivery systems have particular advantages over the simple tea polyphenols.Since there are emerging safety issues and potential local drug overdose effects,it is necessary to determine the actual dosage and pharmacological mechanism of the drug after encapsulating the nanoparticles.展开更多
Tea as the most consumed beverage in the world has received enormous attention for its promoting health benefits.The deleterious effect ofα-dicarbonyls and AGEs formed in Maillard reaction is also a longterm challeng...Tea as the most consumed beverage in the world has received enormous attention for its promoting health benefits.The deleterious effect ofα-dicarbonyls and AGEs formed in Maillard reaction is also a longterm challenge.The connection between the two topics was the main aim of this review,to address and update the antiglycation effect and mechanism of tea and tea polyphenols.By analyzing recent publications,we have covered across chemistry models,cell lines and animal studies.Tea polyphenols,particularly catechins,showed outstanding antiglycation effect by trappingα-dicarbonyl compounds and impeding AGEs formation.Reduction of carbonyl stress brought alleviation to aging,diabetes,and collagen related diseases or complications through regulation of RAGE expression and subsequent MAPK and TGF-βpathway.Therefore,tea polyphenols can serve as promising natural candidates in the treatment and/or prevention of nephropathy,retinopathy,hepatopathy,hyperglycemia and obesity among others,by their potent antiglycation effect.Further studies need to address on aspects like exact mechanisms,solution of detection obstacles,balance of practical usage and harmful effects such as potential flavor damage and toxicity in food,to gain a comprehensive understanding of antiglycation activities of tea polyphenols and its actual application.展开更多
The role of gut microbiota in terms of host health is becoming increasingly important.In this study,the comparative effects of tea polyphenols(TPs)on weight loss and lipid metabolism on conventionalized mice(CVZ)and p...The role of gut microbiota in terms of host health is becoming increasingly important.In this study,the comparative effects of tea polyphenols(TPs)on weight loss and lipid metabolism on conventionalized mice(CVZ)and pseudo germ-free(PGF)mice(treated with antibiotics)were investigated.Our findings revealed that high fat(HF)diet considerably increased the body weight,total fat and upsurge lipid indices in CVZ mice but PGF mice were not sensitive to the effect of HF diet as CVZ mice.After the dietary administration of TP,body weight,perirenal fat and epididymal fat,liver weight,glucose(GLU)level,total chloestrol(TC level),high density lipoprotein-cholesterol(HDL-C)level significantly lowered in PGF mice as compared to CVZ mice group.However,the area of fat cells and triglyceride(TG)level were significantly increased in PGF mice.In CVZ mice,TP intervention resulted in a considerable drop in the Firmicutes/Bacteroides ratio as compared to PGF mice.The intestinal flora of PGF mice was severely reduced after antibiotic treatment,while TP administration restored intestinal diversity;the abundance of Akkermansia and Lactobacillus increased,whereas the abundance of Enterobacteriaceae and Prevotella reduced.Overall,we can assume that PGF obese mice administered with TP have less anti-obesity effects compared to obese CVZ mice.展开更多
Polyphenols are one of the most important metabolites in tea due to their unique biological activities and health benefits,arousing great attention of researchers to investigate biochemical mechanisms of polyphenols d...Polyphenols are one of the most important metabolites in tea due to their unique biological activities and health benefits,arousing great attention of researchers to investigate biochemical mechanisms of polyphenols during tea plant growth,development and tea processing.Although omics has been used as a major analytical platform for tea polyphenol research with some proven merits,a single-omics strategy remains a considerable challenge due to the complexity of biological system and functional processes of tea in each stage of tea production.Recent advances in multi-omics approaches and data analysis have enabled mining and mapping of enormous number of datasets at different biological scales from genotypes to phenotypes of living organisms.These new technologies combining genomics,metagenomics,transcriptomics,proteomics and/or metabolomics can pave a new avenue to address fundamental questions regarding polyphenol formation and changes in tea plants and products.Here,we review recent progresses in single-and multi-omics approaches that have been used in the field of tea polyphenol studies.The perspectives on future research and applications for improvement of tea polyphenols as well as current challenges of multi-omics studies for tea polyphenols are also discussed.展开更多
Objective:To investigate the anti-angiogenic effect of tea polyphenols(TPS)on breast cancer and normal tissues in a mouse model.Methods:Breast cancer was successfully implanted into 48 BALB/c mice,which were then rand...Objective:To investigate the anti-angiogenic effect of tea polyphenols(TPS)on breast cancer and normal tissues in a mouse model.Methods:Breast cancer was successfully implanted into 48 BALB/c mice,which were then randomly divided into a TP oral gavage group,a TP local injection group,a ginsenoside Rg3 group,and a model control group according to a random number table.The tumor inhibitory rates of each group were calculated,while microvessel density(MVD)and the expression of vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF),and tissue inhibitor of metalloproteinase(TIMP-2)were detected by immunohistochemistry.Results:TPs could inhibit the growth of breast cancer xenografts in the mouse model.The tumor inhibition rates of the TP oral gavage and TP local injection groups were 37.43%and 40.94%,respectively.Compared with the model control group,MVD and VEGF and bFGF expression was downregulated(all P<.05),whereas TIMP-2 expression was elevated in the TP oral gavage and TP local injection groups(P=.015 and P=.032).TPs showed no significant effect on MVD and VEGF and TIMP-2 expression in the heart,brain,and kidney of the mouse model.Conclusion:TPs can restrict the growth of breast cancer by specifically inhibiting the angiogenesis of breast tumor tissue while having little effect on the normal tissue of important organs including the heart,brain,and kidney.展开更多
Tea represents an abundant source of naturally occurring polyphenols.Tea polyphenols(TPs)have received growing attentions for its wide consumption in the world,and more importantly its pleiotropic bioeffects for human...Tea represents an abundant source of naturally occurring polyphenols.Tea polyphenols(TPs)have received growing attentions for its wide consumption in the world,and more importantly its pleiotropic bioeffects for human health.After ingestion,TPs may undergo absorption and phase II reaction in the small intestine,and most undigested proportion would be submitted to the colon to interact with gut microbiota.Interactions between gut microbiota and TPs are bidirectional,including not only bacteria-mediated TPs metabolism,e.g.,removal of gallic acid moiety and ring fission to release phenolic acid catabolites,but also TPs-based modification of bacterial profiles.Crosstalk between TPs and gut microbes may benefit for gut barrier function,for example,improvement of the intestinal permeability to alleviate inflammation.Moreover,by reshaping microbial composition and associated metabolites,TPs may exert a systemic protection on host metabolism,which contributes to improve certain chronic metabolic disorders.Given that,further understanding of the metabolic fate of TPs and interplay with gut microbiota as well as potential health-promoting effects are of great significance to development and application of tea and their polyphenolic components in the future as dietary supplements and/or functional ingredients in medical foods.展开更多
基金supported by National Natural Science Foundation of China(31972021)R&D Projects in Key Areas of Guangdong Province(2019B020212003)+4 种基金the Science and Technology Program of Guangzhou,China(202206010177)Guangdong key research and development program(2021B0202060001)Foshan and agricultural academy cooperation projectGuangdong Modern Agriculture project(2022KJ117)Aquatic Products Center Project of GAAS。
文摘Escherichia coli O157:H7 is one of the major foodborne pathogenic bacterial that cause infectious diseases in humans.The previous found that a combination of kojic acid and tea polyphenols exhibited better activity against E.coli O157:H7 than using either alone.This study aimed to explore responses underlying the antibacterial mechanisms of kojic acid and tea polyphenols from the gene level.The functional enrichment analysis by comparing kojic acid and tea polyphenols individually or synergistically against E.coli O157:H7 found that acid resistance systems in kojic acid were activated,and the cell membrane and genomic DNA were destructed in the cells,resulting in“oxygen starvation”.The oxidative stress response triggered by tea polyphenols inhibited both sulfur uptake and the synthesis of ATP,which affected the bacteria's life metabolic process.Interestingly,we found that kojic acid combined with tea polyphenols hindered the uptake of iron that played an essential role in the synthesis of DNA,respiration,tricarboxylic acid cycle.The results suggested that the iron uptake pathways may represent a novel approach for kojic acid and tea polyphenols synergistically against E.coli O157:H7 and provided a theoretical basis for bacterial pathogen control in the food industry.
基金sponsored by the Ningbo Natural Science Foundation(2021J107)。
文摘The interaction between host circadian rhythm and gut microbes through the gut-brain axis provides new clues for tea polyphenols to improve host health.Our present research showed that oolong tea polyphenols(OTP)improved the structural disorder of the intestinal flora caused by continuous darkness,thereby modulating the production of metabolites related to pyruvate metabolism,glycolysis/gluconeogenesis,and tryptophan metabolism to alleviate the steady-state imbalance.After fecal microbiota transplantation from the OTP group,the single-cell transcriptomic analysis revealed that OTP significantly increased the number of hypothalamus cell clusters,up-regulated the number of astrocytes and fibroblasts,and enhanced the expression of circadian rhythm genes Cry2,Per3,Bhlhe41,Nr1d1,Nr1d2,Dbp and Rorb in hypothalamic cells.Our results confirmed that OTP can actively improve the intestinal environmental state as well as internal/peripheral circadian rhythm disorders and cognitive impairment,with potential prebiotic functional characteristics to notably contribute to host health.
基金supported by Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-KJGG-003)Qinglan Plan of Jiangsu Education Department and the National Key Research and Development Program of China(2017YFD0400402).
文摘Membrane pollution caused by separating oily wastewater is a big challenge for membrane separation technology.Recently,plant-/mussel-inspired interface chemistry has received more and more attention.Herein,a high antifouling poly(vinylidene fluoride)(PVDF)membrane,coated with tea polyphenols(TP,extracted from green tea)and 3-amino-propyl-triethoxysilane(APTES),was developed to purify oil-inwater emulsions.ATR-FTIR,XPS and SEM were used to demonstrate the evolution of surface biomimetic hybrid coatings.The performances of the developed membranes were investigated by pure water permeability and oil rejection for various surfactant-stabilized oil-in-water emulsions.The experimental results revealed that the membrane deposited with a mass ratio of 0.1/0.2 exhibited ultrahigh pure water permeability(14570 L·m^(-2)·h^(-1)·bar^(-1),1 bar=0.1 MPa)and isooctane-in-water emulsion permeability(5391 L·m^(-2)·h^(-1)·bar^(-1))with high separation efficiency(>98.9%).Even treated in harsh environment(acidic,alkaline and saline)for seven days,the membrane still maintained considerable underwater oleophobic property(148°–153°).The fabricated plant-inspired biomimetic hybrid membranes with excellent performances light a broad application prospect in the field of oily wastewater treatment.
基金Guangdong Provincial Department of Science and Technology,Science and Technology Plan Project,Journal of Jinan University High-Level Science and Technology Journal Construction Project,No.2021B121020012Guangdong Provincial Administration of Traditional Chinese Medicine,Traditional Chinese Medicine Research Project,No.20213005.
文摘BACKGROUND Di(2-ethylhexyl)phthalate(DEHP)is a common plasticizer known to cause liver injury.Green tea is reported to exert therapeutic effects on heavy metal exposureinduced organ damage.However,limited studies have examined the therapeutic effects of green tea polyphenols(GTPs)on DEHP-induced liver damage.AIM To evaluate the molecular mechanism underlying the therapeutic effects of GTPs on DEHP-induced liver damage.METHODS C57BL/6J mice were divided into the following five groups:Control,model[DEHP(1500 mg/kg bodyweight)],treatment[DEHP(1500 mg/kg bodyweight)+GTP(70 mg/kg bodyweight),oil,and GTP(70 mg/kg bodyweight)]groups.After 8 wk,the liver function,blood lipid profile,and liver histopathology were examined.Differentially expressed micro RNAs(miRNAs)and mRNAs in the liver tissues were examined using high-throughput sequencing.Additionally,functional enrichment analysis and immune infiltration prediction were performed.The miRNA-mRNA regulatory axis was elucidated using the starBase database.Protein expression was evaluated using immunohistochemistry.RESULTS GTPs alleviated DHEP-induced liver dysfunction,blood lipid dysregulation,fatty liver disease,liver fibrosis,and mitochondrial and endoplasmic reticulum lesions in mice.The infiltration of macrophages,mast cells,and natural killer cells varied between the model and treatment groups.mmu-miR-141-3p(a differentially expressed miRNA),Zcchc24(a differentially expressed mRNA),and Zcchc24(a differentially expressed protein)constituted the miRNA-mRNA-protein regulatory axis involved in mediating the therapeutic effects of GTPs on DEHP-induced liver damage in mice.CONCLUSION This study demonstrated that GTPs mitigate DEHP-induced liver dysfunction,blood lipid dysregulation,fatty liver disease,and partial liver fibrosis,and regulate immune cell infiltration.Additionally,an important miRNAmRNA-protein molecular regulatory axis involved in mediating the therapeutic effects of GTPs on DEHP-induced liver damage was elucidated.
基金supported by the National Natural Science Foundation of China(No.82160206 and No.81360152),the Natural Science Foundation of Guangxi Province(No.2018GXNSFAA281234),2019 Guangxi One Thousand Young and Middle-Aged College and University Backbone Teachers Cultivation Program and“Medical Excellence Award”Funded by the Creative Research Development Grant from the First Affiliated Hospital of Guangxi Medical University.
文摘Objective:This study aimed to explore the effects of tea polyphenols(TP)on inflammation of orbital fibroblasts in Graves’ophthalmopathy(GO)and to provide new ideas for GO treatment.Methods:Primary orbital fibroblasts were extracted from orbital adipose/connective tissues of patients with and without GO.Real-time quantitative PCR(RT-qPCR)was used to detect the expression of interleukin(IL)-6,IL-1β,and monocyte chemotactic protein(MCP)-1 in non-GO and GO orbital fibroblasts.The CCK-8 assay was used to determine the appropriate concentration of TP for subsequent experiments.RT-qPCR and enzyme-linked immunosorbent assay(ELISA)were performed to investigate the effects of TP on lipopolysaccharide(LPS)-induced production of inflammatory cytokines.Nuclear factor-κB(NF-κB)expression was measured using Western blotting analysis.NOD-like receptor 3(NLRP3)expression was detected using both Western blotting analysis and immunofluorescence staining.Results:The mRNA levels of IL-6,IL-1β,and MCP-1 in GO orbital fibroblasts were significantly higher than those in non-GO cells.TP treatment significantly inhibited LPS-induced production of inflammatory factors,including IL-6,IL-1β,and MCP-1.TP also inhibited the expression levels of NF-κB and NLRP3.Inflammation in the GO orbital fibroblasts was higher than that in non-GO cells.TP inhibited the production of inflammatory cytokines in GO orbital fibroblasts in vitro through the NF-κB/NLRP3 pathway.Conclusion:These findings suggest that TP may have a potential role in GO treatment.
基金Transformation and Guidance of Scientific and Technological Achievements in Shanxi Province,China(No.202104021301053)Fundamental Research Program of Shanxi Province,China(Nos. 20210302123114 and 202203021211146)+1 种基金Transformation of Scientific and Technological Achievements Programs of Higher Education Institutions in Shanxi Province,China(TSTAP)(No. 2020CG014)Open Project Program of Key Lab for Sport Shoes Upper Materials of Fujian Province,Fujian Huafeng New Material Co.,Ltd.,China(No.S SUM213)。
文摘In order to effectively control the drug-release rate of medical textiles,biodegradable polycaprolactone(PCL) and polyglycolic acid(PGA) were blended at various mass ratios to prepare composite masterbatches for medical textiles.The surface morphology and the chemical structure of the masterbatches were analyzed.The crystallization,mass losses,strengths and drug-release rates of the composite masterbatches at different PCL/PGA mass ratios were explored.The results show that the degradation rate of the PGA carrier is obvious higher than that of the PCL carrier,and PCL,PGA and the tea polyphenol(TP) drug just physically mix without chemical reaction.During the degradation,the strength of the composite masterbatches gradually decreases.In addition,the drug-release rates of composite masterbatches at different mass ratios are different,and the more the PGA in the composite masterbatches,the faster the drug release of the composite masterbatches.The drug-release rate of the composite masterbatches can be controlled by adjusting the contents of PCL and PGA.
文摘In recent years, the incidence of neurodegenerative diseases, mainly Alzheimer’s disease, Parkinson’s disease, vascular dementia, and cerebral ischemia, has been rising gradually, which has a serious impact on the physiological state and quality of life of human beings in old age, and the current clinical drugs are unsatisfactory in terms of therapeutic efficacy and healing, which has made this kind of diseases become a social medical problem. Tea polyphenols are the main functional components of tea and have great potential in neuroprotection. In this paper, we review the research on tea polyphenols in neurodegenerative diseases, with the aim of providing a new entry point for the treatment of neurodegenerative diseases.
基金supports by the Natural Science Foundation of Jilin Province(20210101220JC)Health commission project of Jilin Province(2021LC042).
文摘Tea polyphenols(TP)is a class of polyhydroxy compounds isolated from tea.Modern biological and medical studies have shown that TP has many pharmacological activities,such as anti-inflammatory,anti-virus,anti-oxidation,anti-tumor and anti-radiation.Furthermore,these substances can be used as a potential drug component to positively guide the occurrence and development of certain diseases.Furthermore,because of the activities of TP,such as anti-oxidation and anti-bacteria,it can be applied in food preservation,color preservation,deodorization,and treatment of food processing by-products.Based on the research progress of TP in recent years,this paper summarizes the pharmacological activities of TP and expounds on its application potential in the field of food.In order to provide a theoretical reference for the research,development and utilization of TP.
文摘AIM To investigate protective effects and molecular mechanisms of green tea polyphenols(GTP) on nonalcoholic fatty liver disease(NAFLD) in Zucker fatty(ZF) rats.METHODS Male ZF rats were fed a high-fat diet(HFD) for 2 wk then treated with GTP(200 mg/kg) or saline(5 m L/kg) for 8 wk, with Zucker lean rat as their control. At the end of experiment, serum and liver tissue were collected for measurement of metabolic parameters, alanine aminotransferase(ALT) and aspartate aminotransferase(AST), inflammatory cytokines and hepatic triglyceride and liver histology. Immunoblotting was used to detect phosphorylation of AMP-activated protein kinase(AMPK) acetyl-Co A carboxylase(ACC), and sterol regulatory element-binding protein 1c(SREBP1c). RESULTS Genetically obese ZF rats on a HFD presented with metabolic features of hepatic pathological changes comparable to human with NAFLD. GTP intervention decreased weight gain(10.1%, P = 0.052) and significantly lowered visceral fat(31.0%, P < 0.01). Compared with ZF-controls, GTP treatment significantly reduced fasting serum insulin, glucose and lipids levels. Reduction in serum ALT and AST levels(both P < 0.01) were observed in GTP-treated ZF rats. GTP treatment also attenuated the elevated TNFα and IL-6 in the circulation. The increased hepatic TG accumulation and cytoplasmic lipid droplet were attenuated by GTP treatment, associated with significantly increased expression of AMPK-Thr172(P < 0.05) and phosphorylated ACC and SREBP1c(both P < 0.05), indicating diminished hepatic lipogenesis and triglycerides out flux from liver in GTP treated rats. CONCLUSION The protective effects of GTP against HFD-induced NAFLD in genetically obese ZF rats are positively correlated to reduction in hepatic lipogenesis through upregulating the AMPK pathway.
基金Supported by the Innovative Talents Support Program of Institution of Higher Learning of Liaoning Province,No.2018-478Innovative Talents of Science and Technology Support Program of Young and Middle Aged People of Shenyang,No.RC170446
文摘Colorectal cancer(CRC),a multifactorial disease,is usually induced and developed through complex mechanisms,including impact of diet and lifestyle,genomic abnormalities,change of signaling pathways,inflammatory response,oxidation stress,dysbiosis,and so on.As natural polyphenolic phytochemicals that exist primarily in tea,tea polyphenols(TPs)have been shown to have many clinical applications,especially as anticancer agents.Most animal studies and epidemiological studies have demonstrated that TPs can prevent and treat CRC.TPs can inhibit the growth and metastasis of CRC by exerting the antiinflammatory,anti-oxidative or pro-oxidative,and pro-apoptotic effects,which are achieved by modulations at multiple levels.Many experiments have demonstrated that TPs can modulate several signaling pathways in cancer cells,including the mitogen-activated protein kinase pathway,phosphatidylinositol-3 kinase/Akt pathway,Wnt/β-catenin pathway,and 67 kDa laminin receptor pathway,to inhibit proliferation and promote cell apoptosis.In addition,novel studies have also suggested that TPs can prevent the growth and metastasis of CRC by modulating the composition of gut microbiota to improve immune system and decrease inflammatory responses.Molecular pathological epidemiology,a novel multidisciplinary investigation,has made great progress on CRC,and the further molecular pathological epidemiology research should be developed in the field of TPs and CRC.This review summarizes the existing in vitro and in vivo animal and human studies and potential mechanisms to examine the effects of tea polyphenols on CRC.
基金supported by Hubei Science and Technology Plan Key Project(G2019ABA100)。
文摘Myriad evidence attests to the health-promoting benefits of tea drinking.While there are multiple factors of tea influencing the effective biological properties,tea polyphenols are the most significant and valuable components.The chemical characterization and physical characteristics of tea polyphenols have been comprehensively studied over the previous years.Still the emergence of new chemistry in tea,particularly the property of scavenging reactive carbonyl species(RCS)and the newly discovered flavoalkaloid compounds,has drawn increasing attention.In this review,we summarize recent findings of a new class of compounds in tea-flavonoid alkaloids(flavoalkaloids),which exist in fresh tea leaves and can be generated during the process of post-harvesting,and also postulate the formation mechanism of flavoalkaloids between catechins and theanine-derived Strecker aldehyde.Additionally,we detail the up-to-date research results of tea polyphenols regarding their ability to trap RCS and their in vivo aminated metabolites to suppress advanced glycation ends products(AGEs).We further raise questions to be addressed in the near future,including the synthetic pathways for the generation of flavoalkaloids and AGEs in fresh tea leaves before processing and the concentrations of tea polyphenols that affect their RCS scavenging capability due to their pro-oxidant nature.More intensive research is warranted to elucidate the mechanisms of action underlying the biological activity of flavoalkaloids and the pharmacological application of tea polyphenols in scavenging RCS and impeding detrimental AGEs.
基金supported by the National Research Foundation of Korea(NRF)grant funded by the Korea government(MSIT)(No.NRF-2020R1F1A1073595 and 2021R1A2C2006745)。
文摘Polyphenols,including phenolic acids,flavonoids,and procyanidins,are abundant in food and beverage derived from plants.Tea(Camellia sinensis)is particularly rich in polyphenols(e.g.,catechins,theaflavins,thearubigins,gallic acid,and flavonols),which are thought to contribute to the health benefits of tea.High intake of tea polyphenols has been described to prevent and/or attenuate a variety of chronic pathological conditions like cardiovascular diseases,neurodegenerative diseases,diabetes,and cancer.This review focuses on established antioxidant and anti-inflammatory properties of tea polyphenols and underlying mechanisms of their involvement in inflammatory bowel diseases(IBD).Tea polyphenols act as efficient antioxidants by inducing an endogenous antioxidant defense system and maintaining intracellular redox homeostasis.Tea polyphenols also regulate signaling pathways such as nuclear factor-κB,activator protein 1,signal transducer and activator of transcriptions,and nuclear factor E2-related factor 2,which are associated with IBD development.Accumulating pieces of evidence have indicated that tea polyphenols enhance epithelial barrier function and improve gut microbial dysbiosis,contributing to the management of inflammatory colitis.Therefore,this study suggests that supplementation of tea polyphenols could prevent inflammatory conditions and improve the outcome of patients with IBD.
文摘Tea polyphenols(TPPs)have attracted significant research interest due to their health benefits.However,TPPs are sensitive to certain environmental and gastrointestinal conditions and their oral bioavailability was found to be very low.Delivery systems made of food-grade materials have been reported to improve the shelf-life,bioavailability and bioefficacy of TPPs.This review discusses the chemistry of TPPs;the setbacks of TPPs for application;and the strategies to counteract application limitations by rationally designing delivery systems.An overview of different formulations used to encapsulate TPPs is provided in this study,such as emulsion-based systems(liposome,nanoemulsion,double emulsion,and Pickering emulsion)and nano/microparticles-based systems(protein-based,carbohydrate-based,and bi-polymer based).In addition,the stability,bioavailability and bioactivities of encapsulated TPPs are evaluated by various in vitro and in vivo models.The current findings provide scientific insights in encapsulation approaches for the delivery of TPPs,which can be of great value to TPPs-fortified food products.Further explorations are needed for the encapsulated TPPs in terms of their applications in the real food industry as well as their biological fate and functional pathways in vivo.
基金financially supported by National Key R&D Program of China(No.2017YFC1600402)National Natural Science Foundation of China(No.31772095)the Fundamental Research Funds for the Central Universities,Nankai University(No.63191426).
文摘The metabolic syndrome(MS)has become one of the main problems in public health.Tea polyphenols(TPs),the main bioactive components of tea,has been claimed to have the potential to regulate metabolism and effectively prevent or mitigate the MS.However,many studies into the effects of TPs on MS have provided conflicting findings and the underlying mechanism has been elusive.The predominant TPs in unfermentedand and fermented tea are catechins and oxidized polyphenols(theaflavins and thearubigins),both of which have low bioavailability and reach the colon where most gut microbes inhabit.Gut microbiota has been demonstrated to be tightly associated with host metabolism.The interactions between TPs and gut microbiota will lead to the alterations of gut microbiota composition and the production of metabolites including short chain fatty acids,bile acids,amino acids and TPs derived metabolites,accordingly exerting their biological effects both locally and systemically.This review highlighted the contribution of metabolites and specific gut bacteria in the process of TPs intervention on the MS and further discuss how TPs impact the MS via gut microbiota from the viewpoint of gut organ/tissue axis.
基金Project of National Natural Science Foundation(U1804179)Innovative Technology Team Project of Henan Province"Survey of Dabie Mountain Plant Resources and Research and Utilization of Active Components of Characteristic Plant"(2017083)Nanhu Scholars Program of Xinyang Normal University(2018001)。
文摘This paper elaborated the chemical components,biological metabolism,and progress in the field of drug development of green tea polyphenols,mainly in the prevention and treatment of cancer,neurodegenerative diseases,and diabetes.The potential anti-tumor activity of tea polyphenols can be achieved through intervening in various stages of tumor generation,development,and metastasis.However,the development of tea polyphenols as a therapeutic drug still faces many challenges,such as low bioavailability.Nanoparticle-based drug delivery systems have particular advantages over the simple tea polyphenols.Since there are emerging safety issues and potential local drug overdose effects,it is necessary to determine the actual dosage and pharmacological mechanism of the drug after encapsulating the nanoparticles.
基金funded by Hubei Science and Technology Plan Key Project(G2019ABA100)。
文摘Tea as the most consumed beverage in the world has received enormous attention for its promoting health benefits.The deleterious effect ofα-dicarbonyls and AGEs formed in Maillard reaction is also a longterm challenge.The connection between the two topics was the main aim of this review,to address and update the antiglycation effect and mechanism of tea and tea polyphenols.By analyzing recent publications,we have covered across chemistry models,cell lines and animal studies.Tea polyphenols,particularly catechins,showed outstanding antiglycation effect by trappingα-dicarbonyl compounds and impeding AGEs formation.Reduction of carbonyl stress brought alleviation to aging,diabetes,and collagen related diseases or complications through regulation of RAGE expression and subsequent MAPK and TGF-βpathway.Therefore,tea polyphenols can serve as promising natural candidates in the treatment and/or prevention of nephropathy,retinopathy,hepatopathy,hyperglycemia and obesity among others,by their potent antiglycation effect.Further studies need to address on aspects like exact mechanisms,solution of detection obstacles,balance of practical usage and harmful effects such as potential flavor damage and toxicity in food,to gain a comprehensive understanding of antiglycation activities of tea polyphenols and its actual application.
基金financially support by the Key-Area Research and Development Program of Guangdong Province(2020B020226008,2018b020206001)the National Science Foundation of China(NSFC31171673)。
文摘The role of gut microbiota in terms of host health is becoming increasingly important.In this study,the comparative effects of tea polyphenols(TPs)on weight loss and lipid metabolism on conventionalized mice(CVZ)and pseudo germ-free(PGF)mice(treated with antibiotics)were investigated.Our findings revealed that high fat(HF)diet considerably increased the body weight,total fat and upsurge lipid indices in CVZ mice but PGF mice were not sensitive to the effect of HF diet as CVZ mice.After the dietary administration of TP,body weight,perirenal fat and epididymal fat,liver weight,glucose(GLU)level,total chloestrol(TC level),high density lipoprotein-cholesterol(HDL-C)level significantly lowered in PGF mice as compared to CVZ mice group.However,the area of fat cells and triglyceride(TG)level were significantly increased in PGF mice.In CVZ mice,TP intervention resulted in a considerable drop in the Firmicutes/Bacteroides ratio as compared to PGF mice.The intestinal flora of PGF mice was severely reduced after antibiotic treatment,while TP administration restored intestinal diversity;the abundance of Akkermansia and Lactobacillus increased,whereas the abundance of Enterobacteriaceae and Prevotella reduced.Overall,we can assume that PGF obese mice administered with TP have less anti-obesity effects compared to obese CVZ mice.
文摘Polyphenols are one of the most important metabolites in tea due to their unique biological activities and health benefits,arousing great attention of researchers to investigate biochemical mechanisms of polyphenols during tea plant growth,development and tea processing.Although omics has been used as a major analytical platform for tea polyphenol research with some proven merits,a single-omics strategy remains a considerable challenge due to the complexity of biological system and functional processes of tea in each stage of tea production.Recent advances in multi-omics approaches and data analysis have enabled mining and mapping of enormous number of datasets at different biological scales from genotypes to phenotypes of living organisms.These new technologies combining genomics,metagenomics,transcriptomics,proteomics and/or metabolomics can pave a new avenue to address fundamental questions regarding polyphenol formation and changes in tea plants and products.Here,we review recent progresses in single-and multi-omics approaches that have been used in the field of tea polyphenol studies.The perspectives on future research and applications for improvement of tea polyphenols as well as current challenges of multi-omics studies for tea polyphenols are also discussed.
基金National Natural Science Foundation of China(30472280)and Independent innovation project of Capital Medical Development Research Fund/Traditional Chinese Medicine(SF-2009-III-13).
文摘Objective:To investigate the anti-angiogenic effect of tea polyphenols(TPS)on breast cancer and normal tissues in a mouse model.Methods:Breast cancer was successfully implanted into 48 BALB/c mice,which were then randomly divided into a TP oral gavage group,a TP local injection group,a ginsenoside Rg3 group,and a model control group according to a random number table.The tumor inhibitory rates of each group were calculated,while microvessel density(MVD)and the expression of vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF),and tissue inhibitor of metalloproteinase(TIMP-2)were detected by immunohistochemistry.Results:TPs could inhibit the growth of breast cancer xenografts in the mouse model.The tumor inhibition rates of the TP oral gavage and TP local injection groups were 37.43%and 40.94%,respectively.Compared with the model control group,MVD and VEGF and bFGF expression was downregulated(all P<.05),whereas TIMP-2 expression was elevated in the TP oral gavage and TP local injection groups(P=.015 and P=.032).TPs showed no significant effect on MVD and VEGF and TIMP-2 expression in the heart,brain,and kidney of the mouse model.Conclusion:TPs can restrict the growth of breast cancer by specifically inhibiting the angiogenesis of breast tumor tissue while having little effect on the normal tissue of important organs including the heart,brain,and kidney.
基金financial projects of the National Natural Science Foundation of China(No.81803548)Natural Science Foundation of Tianjin(No.19JCQNJC12400)+1 种基金Hubei Province Technical Innovation Special Project(No.2019ABA100)Tianjin Science and Technology Support Special Project(No.19YFZCSN00010)。
文摘Tea represents an abundant source of naturally occurring polyphenols.Tea polyphenols(TPs)have received growing attentions for its wide consumption in the world,and more importantly its pleiotropic bioeffects for human health.After ingestion,TPs may undergo absorption and phase II reaction in the small intestine,and most undigested proportion would be submitted to the colon to interact with gut microbiota.Interactions between gut microbiota and TPs are bidirectional,including not only bacteria-mediated TPs metabolism,e.g.,removal of gallic acid moiety and ring fission to release phenolic acid catabolites,but also TPs-based modification of bacterial profiles.Crosstalk between TPs and gut microbes may benefit for gut barrier function,for example,improvement of the intestinal permeability to alleviate inflammation.Moreover,by reshaping microbial composition and associated metabolites,TPs may exert a systemic protection on host metabolism,which contributes to improve certain chronic metabolic disorders.Given that,further understanding of the metabolic fate of TPs and interplay with gut microbiota as well as potential health-promoting effects are of great significance to development and application of tea and their polyphenolic components in the future as dietary supplements and/or functional ingredients in medical foods.
