Four polyaniline-tea saponin (PTS) nanocomposites were prepared by an in-situ polymerization with tea saponin (TS) as a biosurfactant,and they were used to remove organic dyes from aqueous solution.The PTS nanocomposi...Four polyaniline-tea saponin (PTS) nanocomposites were prepared by an in-situ polymerization with tea saponin (TS) as a biosurfactant,and they were used to remove organic dyes from aqueous solution.The PTS nanocomposites were characterized by using field emission scanning electron microscopy,the Fourier transform infrared spectroscopy,the Ultraviolet-visible spectroscopy,and the thermogravimetric analysis.The adsorption performances of the PTS nanocomposites for organic dyes were studied by a static adsorption method.The experimental results reveal that adsorption capacities of the PTS nanocomposites are higher than that of pure polyaniline.Especially,the PTS nanocomposites exhibit excellent adsorption performances for anionic dyes because of the electrostatic interaction between the positively charged nitrogen atoms on the PTS chains and the negatively charged sulfonate ions in the anionic dyes.According to the adsorption kinetics and thermodynamics results,the adsorption processes of PTS20 for CR and AB74 follow well with the pseudo second-order and Langmuir isotherm models.It is indicated that TS should be very useful in the preparation of PTS nanocomposite and in removal of toxic dyes from waste water.展开更多
[Objectives] This study was conducted to expand the insect resistance spectrum of tea saponin, and its control effect on Cylas formicarius and the potential as an insecticide for pest control were explored. [Methods] ...[Objectives] This study was conducted to expand the insect resistance spectrum of tea saponin, and its control effect on Cylas formicarius and the potential as an insecticide for pest control were explored. [Methods] The olfactory avoidance rate of C. formicarius to tea saponin aqueous solution was determined by Y-type olfactometer;the feeding avoidance rate of C. formicarius to tea saponin was determined by the selective method;the antifeedant rate of C. formicarius to tea saponin was determined by non-selective method;and the development duration and mortality of C. formicarius under the influence of tea saponin were determined by artificial feeding method. [Results] C. formicarius had no significant olfactory tendency to every concentration of tea saponin, and the olfactory avoidance rate of 20.0% tea saponin aqueous solution was only 9.14%. Tea saponin had a feeding avoidance effect on C. formicarius, and the avoidance rate increased with the increase of tea saponin concentration. At 6 h, the feeding avoidance rates of 0.5%, 1.0%, 5.0%, 10.0% and 20.0% tea saponin on C. formicarius were 58.14%, 77.77%, 88.23%, 95.00% and 97.65 %, respectively;and the feeding avoidance effect at 6 h was significant, and the feeding avoidance rate was higher than that of 1 h. Tea saponin had a significant antifeedant effect on C. formicarius, and the longer the feeding time, the higher the antifeedant rate. At 72 h, the antifeedant rates of 0.5%, 1.0%, 5.0%, 10.0 % and 20.0% tea saponin to C. formicarius were 63.01%, 67.54%, 97.14 %, 96.42% and 98.57%, respectively. The larval development duration of C. formicarius was shortened with the increase of tea saponin concentration, and the larval death occurred. The development duration of larvae under the influence of 1.0% tea saponin was the shortest, which was 4.01 d shorter than that of the control, and the mortality was the highest, which was 26.65%. [Conclusions] Tea saponin had neither olfactory avoidance effect nor olfactory attracting effect to C. formicarius, but had obvious feeding avoidance effect and strong antifeedant effect. Tea saponin can shorten the development duration of the larvae of C. formicarius and cause the death of the larvae.展开更多
The effect of tea saponins (TS) on rumen fermentation and methane emission was examined using an in vitro gas production technique named Reading Pressure Technique. Three levels of TS addition (0, 0.2, 0.4 mg/ml) were...The effect of tea saponins (TS) on rumen fermentation and methane emission was examined using an in vitro gas production technique named Reading Pressure Technique. Three levels of TS addition (0, 0.2, 0.4 mg/ml) were evaluated in the faunated and defaunated rumen fluid. Compared to the control, TS addition decreased the 24 h gas production in the faunated rumen fluid, but had a minor effect on gas yield in the defaunated rumen fluid. The TS significantly reduced methane production in vitro. In the faunated rumen fluid, 0.2 or 0.4 mg/ml TS decreased the 24 h methane emission by 12.7% or 14.0%, respectively. Rumen fluid pH value was affected neither by TS addition nor by defaunation. The TS addition had only minor effects on volatile fatty acids, but the yield and pattern of volatile fatty acids were greatly affected by defaunation. While the molar proportion of acetate was not affected by defaunation, the propionate was significantly increased and the butyrate significantly decreased. Ammonia-N concentration and microbial protein yield were influenced by TS inclusion and defaunation. Inclusion of 0.4 mg/ml TS increased the microbial protein mass by 18.4% and 13.8% and decreased the ammonia-N concentration by 8.3% and 19.6% in the faunated and defaunated rumen fluid, respectively. Protozoa counts were significantly reduced by TS inclusion. The current study demonstrated the beneficial effect of TS on methane production and rumen fermentation, and indicated that this may be due to the effect of the associated depression on protozoa counts.展开更多
This paper summarized the quantitative analysis methods of tea saponin,including gravimetry,colorimetry,fluorescence spectrophotometry,thin layer scanning and HPLC method.Through the summary of quantitative analysis m...This paper summarized the quantitative analysis methods of tea saponin,including gravimetry,colorimetry,fluorescence spectrophotometry,thin layer scanning and HPLC method.Through the summary of quantitative analysis methods,this paper tried to compare different quantitative determination methods so as to provide a guidance to establish the standard quantitative analysis method of tea saponin from various sources.展开更多
Background: This study investigated changes in rumen protozoal and methanogenic communities, along with the correlations among microbial taxa and methane(CH_4) production of six Belmont Red Composite beef steers fed t...Background: This study investigated changes in rumen protozoal and methanogenic communities, along with the correlations among microbial taxa and methane(CH_4) production of six Belmont Red Composite beef steers fed tea seed saponins(TSS). Animals were fed in three consecutive feeding periods, a high-grain basal diet for 14 d(BD period) then a period of progressive addition of TSS to the basal diet up to 30 g/d for 20 d(TSS period), followed by the basal diet for 13 d without TSS(BDP post-control period).Results: The study found that TSS supplementation decreased the amount of the protozoal genus Entodinium and increased Polyplastron and Eudiplodinium genera. During BDP period, the protozoa community of steers did not return to the protozoal profiles observed in BD period, with higher proportions of Metadinium and Eudiplodinium and lower Isotricha. The addition of TSS was found to change the structure of methanogen community at the subgenus level by decreasing the abundance of methanogens in the SGMT clade and increasing the abundance of methanogens in the RO clade. The correlation analysis indicated that the abundance of SGMT clade methanogens were positively correlated with Isotricha, and Isotricha genus and SGMT clade methanogens were positively correlated with CH_4 production. While RO clade were positively correlated with the proportion of Metadinium genus, which was negatively correlated with CH_4 emission.Conclusions: These results suggest that different genera of rumen protozoa ciliates appear to be selectively inhibited by TSS, and the change in methanogen community at the subgenus level may be due to the mutualistic relationships between methanogens and rumen ciliates.展开更多
采用磁性固定酶技术从粗茶皂素中靶向分离出了乙醇脱氢酶的相关活性组分(ADH-related active ingredients,AAI)。为了揭示AAI的表面活性,通过表面张力法测定其临界胶束浓度;使用量角法测量其接触角;采用单因素实验探究浓度、水质硬度、p...采用磁性固定酶技术从粗茶皂素中靶向分离出了乙醇脱氢酶的相关活性组分(ADH-related active ingredients,AAI)。为了揭示AAI的表面活性,通过表面张力法测定其临界胶束浓度;使用量角法测量其接触角;采用单因素实验探究浓度、水质硬度、pH等因素对泡沫性能的影响;同时采用分光光度法研究AAI的增溶能力,并与不同表面活性剂比较乳化和去污能力。结果表明:AAI具有降低液体的表面张力的作用,且该作用随AAI浓度增加而增强,AAI的临界胶束浓度在0.3%左右。AAI的润湿性能良好,在浓度0.1%~1.0%的范围内,接触角始终小于90°,且随浓度增加而减小。AAI的浓度越高,其起泡性越强,同时泡沫稳定性较好,具有抵抗硬水和酸碱的能力,在50℃时AAI的起泡性能最好。AAI有一定的增溶能力,对典型难溶有机物苯的增溶能力为7 mL·g^(-1)。AAI的乳化能力良好,单独使用时去污能力不强,与十二烷基苯磺酸钠(SDBS)组合使用效果可明显提升。展开更多
文摘Four polyaniline-tea saponin (PTS) nanocomposites were prepared by an in-situ polymerization with tea saponin (TS) as a biosurfactant,and they were used to remove organic dyes from aqueous solution.The PTS nanocomposites were characterized by using field emission scanning electron microscopy,the Fourier transform infrared spectroscopy,the Ultraviolet-visible spectroscopy,and the thermogravimetric analysis.The adsorption performances of the PTS nanocomposites for organic dyes were studied by a static adsorption method.The experimental results reveal that adsorption capacities of the PTS nanocomposites are higher than that of pure polyaniline.Especially,the PTS nanocomposites exhibit excellent adsorption performances for anionic dyes because of the electrostatic interaction between the positively charged nitrogen atoms on the PTS chains and the negatively charged sulfonate ions in the anionic dyes.According to the adsorption kinetics and thermodynamics results,the adsorption processes of PTS20 for CR and AB74 follow well with the pseudo second-order and Langmuir isotherm models.It is indicated that TS should be very useful in the preparation of PTS nanocomposite and in removal of toxic dyes from waste water.
基金Supported by National Modern Agricultural Industry Technology System Guangxi Industry Potato Innovation Team(nycytxgxcxtd-11-01)Guangxi Science and Technology Planning Project(GK AB16380046,GK AB18221101)
文摘[Objectives] This study was conducted to expand the insect resistance spectrum of tea saponin, and its control effect on Cylas formicarius and the potential as an insecticide for pest control were explored. [Methods] The olfactory avoidance rate of C. formicarius to tea saponin aqueous solution was determined by Y-type olfactometer;the feeding avoidance rate of C. formicarius to tea saponin was determined by the selective method;the antifeedant rate of C. formicarius to tea saponin was determined by non-selective method;and the development duration and mortality of C. formicarius under the influence of tea saponin were determined by artificial feeding method. [Results] C. formicarius had no significant olfactory tendency to every concentration of tea saponin, and the olfactory avoidance rate of 20.0% tea saponin aqueous solution was only 9.14%. Tea saponin had a feeding avoidance effect on C. formicarius, and the avoidance rate increased with the increase of tea saponin concentration. At 6 h, the feeding avoidance rates of 0.5%, 1.0%, 5.0%, 10.0% and 20.0% tea saponin on C. formicarius were 58.14%, 77.77%, 88.23%, 95.00% and 97.65 %, respectively;and the feeding avoidance effect at 6 h was significant, and the feeding avoidance rate was higher than that of 1 h. Tea saponin had a significant antifeedant effect on C. formicarius, and the longer the feeding time, the higher the antifeedant rate. At 72 h, the antifeedant rates of 0.5%, 1.0%, 5.0%, 10.0 % and 20.0% tea saponin to C. formicarius were 63.01%, 67.54%, 97.14 %, 96.42% and 98.57%, respectively. The larval development duration of C. formicarius was shortened with the increase of tea saponin concentration, and the larval death occurred. The development duration of larvae under the influence of 1.0% tea saponin was the shortest, which was 4.01 d shorter than that of the control, and the mortality was the highest, which was 26.65%. [Conclusions] Tea saponin had neither olfactory avoidance effect nor olfactory attracting effect to C. formicarius, but had obvious feeding avoidance effect and strong antifeedant effect. Tea saponin can shorten the development duration of the larvae of C. formicarius and cause the death of the larvae.
基金Project (No. 12665/R0) supported partly by Co-ordinated ResearchProjects from Joint FAO/IAEA Division, IAEA
文摘The effect of tea saponins (TS) on rumen fermentation and methane emission was examined using an in vitro gas production technique named Reading Pressure Technique. Three levels of TS addition (0, 0.2, 0.4 mg/ml) were evaluated in the faunated and defaunated rumen fluid. Compared to the control, TS addition decreased the 24 h gas production in the faunated rumen fluid, but had a minor effect on gas yield in the defaunated rumen fluid. The TS significantly reduced methane production in vitro. In the faunated rumen fluid, 0.2 or 0.4 mg/ml TS decreased the 24 h methane emission by 12.7% or 14.0%, respectively. Rumen fluid pH value was affected neither by TS addition nor by defaunation. The TS addition had only minor effects on volatile fatty acids, but the yield and pattern of volatile fatty acids were greatly affected by defaunation. While the molar proportion of acetate was not affected by defaunation, the propionate was significantly increased and the butyrate significantly decreased. Ammonia-N concentration and microbial protein yield were influenced by TS inclusion and defaunation. Inclusion of 0.4 mg/ml TS increased the microbial protein mass by 18.4% and 13.8% and decreased the ammonia-N concentration by 8.3% and 19.6% in the faunated and defaunated rumen fluid, respectively. Protozoa counts were significantly reduced by TS inclusion. The current study demonstrated the beneficial effect of TS on methane production and rumen fermentation, and indicated that this may be due to the effect of the associated depression on protozoa counts.
基金supported by National Key Technology R&D Program in the 12th Five Year Plan of China (Project No.2012BAD36B06-2)
文摘This paper summarized the quantitative analysis methods of tea saponin,including gravimetry,colorimetry,fluorescence spectrophotometry,thin layer scanning and HPLC method.Through the summary of quantitative analysis methods,this paper tried to compare different quantitative determination methods so as to provide a guidance to establish the standard quantitative analysis method of tea saponin from various sources.
基金supported by grants from the Natural Science Foundation of China (31272472)project grants from Meat & Livestock Australia,Fisheries and Forestry in Australia and Commonwealth Scientific and Industrial Research Organization (CSIRO)。
文摘Background: This study investigated changes in rumen protozoal and methanogenic communities, along with the correlations among microbial taxa and methane(CH_4) production of six Belmont Red Composite beef steers fed tea seed saponins(TSS). Animals were fed in three consecutive feeding periods, a high-grain basal diet for 14 d(BD period) then a period of progressive addition of TSS to the basal diet up to 30 g/d for 20 d(TSS period), followed by the basal diet for 13 d without TSS(BDP post-control period).Results: The study found that TSS supplementation decreased the amount of the protozoal genus Entodinium and increased Polyplastron and Eudiplodinium genera. During BDP period, the protozoa community of steers did not return to the protozoal profiles observed in BD period, with higher proportions of Metadinium and Eudiplodinium and lower Isotricha. The addition of TSS was found to change the structure of methanogen community at the subgenus level by decreasing the abundance of methanogens in the SGMT clade and increasing the abundance of methanogens in the RO clade. The correlation analysis indicated that the abundance of SGMT clade methanogens were positively correlated with Isotricha, and Isotricha genus and SGMT clade methanogens were positively correlated with CH_4 production. While RO clade were positively correlated with the proportion of Metadinium genus, which was negatively correlated with CH_4 emission.Conclusions: These results suggest that different genera of rumen protozoa ciliates appear to be selectively inhibited by TSS, and the change in methanogen community at the subgenus level may be due to the mutualistic relationships between methanogens and rumen ciliates.
文摘采用磁性固定酶技术从粗茶皂素中靶向分离出了乙醇脱氢酶的相关活性组分(ADH-related active ingredients,AAI)。为了揭示AAI的表面活性,通过表面张力法测定其临界胶束浓度;使用量角法测量其接触角;采用单因素实验探究浓度、水质硬度、pH等因素对泡沫性能的影响;同时采用分光光度法研究AAI的增溶能力,并与不同表面活性剂比较乳化和去污能力。结果表明:AAI具有降低液体的表面张力的作用,且该作用随AAI浓度增加而增强,AAI的临界胶束浓度在0.3%左右。AAI的润湿性能良好,在浓度0.1%~1.0%的范围内,接触角始终小于90°,且随浓度增加而减小。AAI的浓度越高,其起泡性越强,同时泡沫稳定性较好,具有抵抗硬水和酸碱的能力,在50℃时AAI的起泡性能最好。AAI有一定的增溶能力,对典型难溶有机物苯的增溶能力为7 mL·g^(-1)。AAI的乳化能力良好,单独使用时去污能力不强,与十二烷基苯磺酸钠(SDBS)组合使用效果可明显提升。
