The insulin-like growth factor Ⅰ(IGF-Ⅰ) gene of triangular bream (Megalobrama terminalis)(GenBank No.AY247412)(Tb)was cloned for the first time from liver by RT-PCR. Thenucleotide sequence analysis showed the Tb IGF...The insulin-like growth factor Ⅰ(IGF-Ⅰ) gene of triangular bream (Megalobrama terminalis)(GenBank No.AY247412)(Tb)was cloned for the first time from liver by RT-PCR. Thenucleotide sequence analysis showed the Tb IGF-ⅠcDNA consisted of 486 nucleotides andencoded 117 amino acids including B, C, A, D and E five domains. Analysis of E-domainindicated that cloned Tb IGF-Ⅰbelonged to IGF-ⅠEa-2 subtype. Identity analysis showedthe IGF-Ⅰnucleotide sequence shared 99.8% homology with bluntnose bream, 88.8% withgrass carp, 85.8% with common carp; the pre-IGF-Ⅰamine acid sequence shared 99.4% withbluntnose bream, 88.8% with grass carp, 85.4% homology with common carp. In the CyprinusCarpio, the higher homology of nucleotide sequence and amino acid sequence in IGF-Ⅰshowed that the closer relationship the fishes have. These results could provide basicdata for the research on Tb germplasm and the development and utilization of biologicalfeed additives.展开更多
Anxiety is a common disease in the modern society which significantly affects people’s daily lives and function,thus it has become an increasingly highlighted issue.Anxiety is regulated by neural circuits in the brai...Anxiety is a common disease in the modern society which significantly affects people’s daily lives and function,thus it has become an increasingly highlighted issue.Anxiety is regulated by neural circuits in the brain.Therefore,the basal mechanism of anxiety has been studied,especially research based on the related neural circuits.For a long time,due to the limitations of science and technology,there was no breakthrough in research regarding anxiety.However,in recent years,due to the progress of technology,the research on anxiety neural circuits has made great progress.For example,the interaction among various brain regions,such as the central nucleus of the amygdala(CeA),the ventral tegmental area(VTA),the ventral hippocampus(vHPC),and so on.This article focuses on three brain regions:including BLA,BNST,and VTA,and illustrate their different roles and mechanisms in regulating anxiety.On this basis,this intensive study of anxiety will further promote the progress of anxiety research and provide therapeutic targets for the related treatment.展开更多
文摘The insulin-like growth factor Ⅰ(IGF-Ⅰ) gene of triangular bream (Megalobrama terminalis)(GenBank No.AY247412)(Tb)was cloned for the first time from liver by RT-PCR. Thenucleotide sequence analysis showed the Tb IGF-ⅠcDNA consisted of 486 nucleotides andencoded 117 amino acids including B, C, A, D and E five domains. Analysis of E-domainindicated that cloned Tb IGF-Ⅰbelonged to IGF-ⅠEa-2 subtype. Identity analysis showedthe IGF-Ⅰnucleotide sequence shared 99.8% homology with bluntnose bream, 88.8% withgrass carp, 85.8% with common carp; the pre-IGF-Ⅰamine acid sequence shared 99.4% withbluntnose bream, 88.8% with grass carp, 85.4% homology with common carp. In the CyprinusCarpio, the higher homology of nucleotide sequence and amino acid sequence in IGF-Ⅰshowed that the closer relationship the fishes have. These results could provide basicdata for the research on Tb germplasm and the development and utilization of biologicalfeed additives.
文摘Anxiety is a common disease in the modern society which significantly affects people’s daily lives and function,thus it has become an increasingly highlighted issue.Anxiety is regulated by neural circuits in the brain.Therefore,the basal mechanism of anxiety has been studied,especially research based on the related neural circuits.For a long time,due to the limitations of science and technology,there was no breakthrough in research regarding anxiety.However,in recent years,due to the progress of technology,the research on anxiety neural circuits has made great progress.For example,the interaction among various brain regions,such as the central nucleus of the amygdala(CeA),the ventral tegmental area(VTA),the ventral hippocampus(vHPC),and so on.This article focuses on three brain regions:including BLA,BNST,and VTA,and illustrate their different roles and mechanisms in regulating anxiety.On this basis,this intensive study of anxiety will further promote the progress of anxiety research and provide therapeutic targets for the related treatment.
