<span style="font-family:'';font-size:10pt;"><span style="font-size:12px;font-family:Verdana;">Pathogenic spore-forming bacteria pose high risks to healthcare settings, as well ...<span style="font-family:'';font-size:10pt;"><span style="font-size:12px;font-family:Verdana;">Pathogenic spore-forming bacteria pose high risks to healthcare settings, as well as in the food and beverage industries. We reported recently that novel alcohol-based formulations containing plant-derived compounds, including epigallocatechin-3-gallate-palmitate (EGCG-P), a green tea polyphenol ester, provide > 99.99% inactivation of bacterial spores within 60 sec. Based on recently published data from our group and others, we hypothesize that a combination of EGCG-P and alcohol formulated with other plant-derived ingredients would achieve high sporicidal efficacy against a wide spectrum of bacterial spores and can provide novel hand hygiene methods against bacterial spores without toxicity. The objectives of the current study were to optimize </span><span style="font-size:12px;font-family:Verdana;">two novel formulations with combinations of glycerol, citric acid, and EGCG-P</span><span style="font-size:12px;font-family:Verdana;"> to increase sporicidal activity and explore the rapid inactivation mechanisms and suitability for sporicidal products with broad-spectrum activities against aerobic and anaerobic bacterial spores. Methods included suspension testing of two formulations against spores from </span><i><span style="font-size:12px;font-family:Verdana;">Bacillus cereus</span></i><span style="font-size:12px;font-family:Verdana;"> and </span><i><span style="font-size:12px;font-family:Verdana;">Clostridium sporogenes</span></i><span style="font-size:12px;font-family:Verdana;">, quantification of spore germination, and scanning electron microscopy. The results demonstrated that these novel formulations were able to reduce spore germination by >99.999% after 30 sec exposure in </span><span style="font-size:12px;font-family:Verdana;">suspension tests, and rapidly caused physical damage to the spores. Additional</span></span><span style="font-family:'';font-size:10pt;"> </span><span style="line-height:1.5;font-family:Verdana;">studies</span><span style="font-family:'';font-size:10pt;"> </span><span style="line-height:1.5;font-family:Verdana;">are </span><span style="line-height:1.5;font-family:Verdana;">warranted to determine the suitability of the novel formulations for future hand hygiene use.展开更多
文摘<span style="font-family:'';font-size:10pt;"><span style="font-size:12px;font-family:Verdana;">Pathogenic spore-forming bacteria pose high risks to healthcare settings, as well as in the food and beverage industries. We reported recently that novel alcohol-based formulations containing plant-derived compounds, including epigallocatechin-3-gallate-palmitate (EGCG-P), a green tea polyphenol ester, provide > 99.99% inactivation of bacterial spores within 60 sec. Based on recently published data from our group and others, we hypothesize that a combination of EGCG-P and alcohol formulated with other plant-derived ingredients would achieve high sporicidal efficacy against a wide spectrum of bacterial spores and can provide novel hand hygiene methods against bacterial spores without toxicity. The objectives of the current study were to optimize </span><span style="font-size:12px;font-family:Verdana;">two novel formulations with combinations of glycerol, citric acid, and EGCG-P</span><span style="font-size:12px;font-family:Verdana;"> to increase sporicidal activity and explore the rapid inactivation mechanisms and suitability for sporicidal products with broad-spectrum activities against aerobic and anaerobic bacterial spores. Methods included suspension testing of two formulations against spores from </span><i><span style="font-size:12px;font-family:Verdana;">Bacillus cereus</span></i><span style="font-size:12px;font-family:Verdana;"> and </span><i><span style="font-size:12px;font-family:Verdana;">Clostridium sporogenes</span></i><span style="font-size:12px;font-family:Verdana;">, quantification of spore germination, and scanning electron microscopy. The results demonstrated that these novel formulations were able to reduce spore germination by >99.999% after 30 sec exposure in </span><span style="font-size:12px;font-family:Verdana;">suspension tests, and rapidly caused physical damage to the spores. Additional</span></span><span style="font-family:'';font-size:10pt;"> </span><span style="line-height:1.5;font-family:Verdana;">studies</span><span style="font-family:'';font-size:10pt;"> </span><span style="line-height:1.5;font-family:Verdana;">are </span><span style="line-height:1.5;font-family:Verdana;">warranted to determine the suitability of the novel formulations for future hand hygiene use.
基金Tianshan Youth Technology Talents Training Program(2017Q032)Doctoral Starting Funds of Xinjiang Institute of Engineering(2016xgy351812)Education Department of Xinjiang Uygur Autonomous Region(2017jyt012006)
