[Objectives] The aim was to determine the moisture,ash and extract contents in Leontopodium franchetii Beauv. from different localities. [Methods] The contents of moisture,total ash,acid-insoluble ash and extract in L...[Objectives] The aim was to determine the moisture,ash and extract contents in Leontopodium franchetii Beauv. from different localities. [Methods] The contents of moisture,total ash,acid-insoluble ash and extract in L. franchetii from different localities were determined according to the methods described in Chinese Pharmacopoeia( 2015 Edition). [Results] In the 10 samples,the contents of moisture were all less than or equal to 15. 0%; the contents of total ash were all below 12. 0%; and the contents of acid-insoluble ash were all less than 3. 0%. The contents of water-soluble extract( by cold-soaked extraction method) in the samples were all below 12. 0% except those from Hongyuan Prairie of Sichuan Province and Xinglong Mountain in Lanzhou City,Gansu Province. [Conclusions]The contents of the four indicators measured in this experiment varied by small margins,indicating that the quality was relatively stable. This study provides theoretical data for the revision of the quality standards for L. franchetii.展开更多
Nucleoside is the main class of active components in Cordyceps sinensis. Thin-layer chromatography (TLC) is one of the most commonly used methods in pharmacopoeias for analyzing chemical components of herbal medicine....Nucleoside is the main class of active components in Cordyceps sinensis. Thin-layer chromatography (TLC) is one of the most commonly used methods in pharmacopoeias for analyzing chemical components of herbal medicine. Since the isocratic elution method cannot be applied successfully in TLC analysis for separating all the nucleoside components, the stepwise gradient elution has been developed in this work to separate eight nucleoside standards with success. In this way, quantitative analyses of the samples of Cordyceps sinensis were achieved via the pro-posed TLC procedure coupled with the scanning densitometric techniques of CAMAG and TLCQA methods for qualitative and quantitative analysis.展开更多
In this study,a high performance thin-layer chromatography/single quadrupole mass spectrometry QDa(HPTLC-QDa)method for robust authentication of Ganoderma lucidum,a popular and valuable herbal medicine,has been develo...In this study,a high performance thin-layer chromatography/single quadrupole mass spectrometry QDa(HPTLC-QDa)method for robust authentication of Ganoderma lucidum,a popular and valuable herbal medicine,has been developed.This method is simple and practical,which allows direct generation of characteristic mass spectra from the HPTLC plates automatically with the application of in situ solvent desorption interface.The HPTLC silica gel plates were developed with toluene-ethyl formate-formic acid(5:5:0.2,V/V)and all bands were transferred to QDa system directly in situ using 80%methanol with 0.1%formic acid as desorption solvent.The acquired HPTLC-QDa spectra showed that luminous yellow band b3,containing ganoderic acid B/G/H and ganodeneric acid B,the major active components of Ganoderma,could be found only in G.lucidum and G.lucidum(Antler-shaped),but not in G.sinense and G.applanatum.Moreover,bands b13 and b14 with m/z 475/477 and m/z 475/491/495,respectively,could be detected in G.lucidum(Antler-shaped),but not in G.lucidum,thus allowing simple and robust authentication of G.lucidum with confused species.This method is proved to be simple,practical and reproducible,which can be extended to analyze other herbal medicines.展开更多
Objective:Belamcandae Rhizoma and Iridis Tectori Rhizoma are easily confused with each other.The main objective of this study is to distinguish them using chemical analysis.Materials and Methods:Thin-layer chromatogra...Objective:Belamcandae Rhizoma and Iridis Tectori Rhizoma are easily confused with each other.The main objective of this study is to distinguish them using chemical analysis.Materials and Methods:Thin-layer chromatography(TLC)and high-performance liquid chromatography(HPLC)fingerprint methods were established to compare the chemical profile,while HPLC quantitation was used to determine the contents of three isoflavones in thirty batches of Belamcandae Rhizoma and Iridis Tectori Rhizoma samples.Results:The two herbs could be distinguished by TLC using acetic acid-n hexane-ethyl acetate(1:90:80 v/v/v)as the mobile phase,according to the fluorescent band under 366 nm at R_(f) 0.2.In total,12 compounds were identified in the 24-min HPLC fingerprint.The similarity coefficient between the two herbs was 0.54±0.01.Mangiferin(1),tectoridin(2),iridin(3),irigenin(5),irisflorentin(6),and iristectorin A(9)were the main peaks in Belamcandae Rhizoma,while tectoridin(2)and tectorigenin(4)were the major peaks in Iridis Tectori Rhizoma.The contents of 2 in Iridis Tectori Rhizoma(2.50±0.20%)were 8.93 times higher than that of Belamcandae Rhizoma(0.28±0.08%),while the ones of 5 and 6 were slightly lower in Iridis Tectori Rhizoma.Conclusions:The study established fast and effective methods to distinguish Belamcandae Rhizoma from Iridis Tectori Rhizoma.展开更多
As a widely used food preservative,methyl paraben was experimentally evidenced with serious hormonelike adverse effects.Herein,a high performance thin-layer chromatography platformed bioluminescent bioautography and i...As a widely used food preservative,methyl paraben was experimentally evidenced with serious hormonelike adverse effects.Herein,a high performance thin-layer chromatography platformed bioluminescent bioautography and image analysis for the selective quantification and confirmation of methyl paraben was proposed and validated in vinegar and coconut juice.First,the detectability of the bioautography to the analyte on different layer materials was estimated,revealing that normal silica gel was the best choice.After that,the liquid of sample extract and working solution were separated to overcome the background noises due to co-extracted matrices.The separation result was then coupled to the optimized bioautography,enabling instant and straightforward screening of the targeted conpound.For accurate quantification,bioluninescent inhibition pattern caused by the analyte was processed by image analysis,giving useful sensitivity(LOD>16 mg/kg),precision(RSD<10.1%)and accuracy(spike-recovery rate 76.9%-112.2%).Finally,the suspected result was confirmed by determining its MS fingerprint,further strengthening the reliability of screening.展开更多
Objective: To determine the thin-layer chromatography(TLC) fingerprint profiles and to evaluate the in vitro antioxidant activity of ethanol extracts of Muntingia calabura(M. calabura) leaves and stems.Methods: The le...Objective: To determine the thin-layer chromatography(TLC) fingerprint profiles and to evaluate the in vitro antioxidant activity of ethanol extracts of Muntingia calabura(M. calabura) leaves and stems.Methods: The leaves and stems were extracted using ethanol as solvent. The TLC separation of the phytochemical constituents of the leaf and ethanol extracts was carried out in ethyl acetate: n-hexane and chloroform: ethyl acetate mobile phase systems.Distinct spots were visualized under visible light, UV 254 nm, UV 366 nm and after spraying with vanillin-sulfuric acid. The 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging assay was used to evaluate the antioxidant activity of the extracts.Results: Both the leaf and stem ethanol extracts at 4 mg/mL exhibited 2,2-diphenyl-1-picrylhydrazyl inhibition of more than 90%, relative to gallic acid. The results of TLC showed that the degree of resolution between the constituent spots was comparable between the two mobile phase systems using the different visualization wavelengths. Under the 254 nm visualization, few spots were observed in leaf and stem extracts. Visualization at 366 nm yielded the greatest number of observable spots of various colors in both leaf and stem extracts. More spots were visualized upon post-derivatization with vanillinsulfuric acid in the TLC chromatograms using chloroform: ethyl acetate mobile phase,compared to those in ethyl acetate: n-hexane mobile phase.Conclusions: M. calabura exhibited very high antioxidant activity in its leaves and stems ethanol extracts, both of which are used in traditional medicine. The TLC results demonstrated the presence of diverse secondary metabolites in the leaf and stem ethanol extracts, indicating that the antioxidant activity, including other bioactivities may be attributed to these phytochemical constituents. This paper has reported for the first time the TLC fingerprinting of M. calabura using visible light, UV 254 nm, UV 366 and postderivatization with vanillin-spray to visualize separate spots on TLC plates.展开更多
Aflatoxin B1 is a mycotoxin that can contaminate a wide feedstuffs variety. Ingestion of contaminated feed by poultry can lead to impaired health and zootechnical performances but also a human diet safety problem rela...Aflatoxin B1 is a mycotoxin that can contaminate a wide feedstuffs variety. Ingestion of contaminated feed by poultry can lead to impaired health and zootechnical performances but also a human diet safety problem related to residues presence in animal origin products. Aflatoxin B1 contamination of poultry feed samples marketed in Dakar city and in peri-urban areas (Gorom, Sangalkam) was studied. A total of 15 samples were collected from Dakar city markets as well as from poultry farms in Gorom and Sangalkam areas. Aflatoxin B1 quantification was performed by high performance liquid chromatography and thin-layer chromatography. HPLC results showed that all samples were contaminated with levels ranging from 0.15 to 22 ppb, 0.099 to 2.05 ppb and 0.099 to 4.95 ppb respectively for Gorom, Sangalkam and Dakar. Only the finishing feed from Gorom had an aflatoxin B1 level above the maximum limit set by regulations. TLC is a suitable method for aflatoxins detection. However, it was associated with overestimation for aflatoxin B1 quantification. Results suggest that poultry feed represents a real source of human diet contamination. In addition, HPLC remains the most reliable quantification technique for quality control.展开更多
[Objectives]To establish a method for caffeic acid content determination and thin-layer chromatography identification of the Zhuang medicine Argyreia acuta Lour.[Methods]Silica gel GF254(thin-layer plate),toluene-form...[Objectives]To establish a method for caffeic acid content determination and thin-layer chromatography identification of the Zhuang medicine Argyreia acuta Lour.[Methods]Silica gel GF254(thin-layer plate),toluene-formic acid-ethyl acetate(V∶V∶V=5∶1.2∶3)(developing agent)and 365 nm ultraviolet light were used for thin-layer chromatography identification.Under the chromatographic conditions of column of Thermo SCIENTIFIC Hypersil C18(5μm,4.60 mm×250 mm),mobile phase of methanol-0.1%phosphoric acid(23∶77),detection wavelength of 320 nm,column temperature of 30℃,sample size of 10μL and flow rate of 1.0 mL/min,the content of caffeic acid in A.acuta Lour was determined.[Results]Caffeic acid can be detected in thin layer chromatography,with strong specificity and clear spots.When the sample size of caffeic acid is within the range of 0.0151-0.4530μg(R^(2)=0.9999),the content of caffeic acid showed a relatively good linear relationship with the peak area.The average recovery rate of A.acuta Lour was 99.94%(RSD=2.68%),97.56%(RSD=1.57%)and 99.79%(RSD=2.05%),respectively.[Conclusions]This method can effectively identify A.acuta Lour,and can accurately determine the content of caffeic acid in A.acuta Lour.It has characteristics of high accuracy,high precision,good color rendering stability,good reproducibility and fast analysis speed.展开更多
[Objectives]This study aimed to establish a qualitative identification and content determination method for psoralen in Ficus pandurata Hance and compare the psoralen contents in roots,stems and leaves of F.pandurate ...[Objectives]This study aimed to establish a qualitative identification and content determination method for psoralen in Ficus pandurata Hance and compare the psoralen contents in roots,stems and leaves of F.pandurate Hance and Ficus pandurata Hance var.holophylla Migo.[Methods]Thin-layer chromatography(TLC)was used for qualitative identification,and the content of psoralen was determined by high-performance liquid chromatography.The chromatographic conditions were as follows:column,Agilent ZORBAX Eclipse Plus C18(250 mm×4.6 mm,5μm);mobile phase,methanol-water(55∶45);flow rate,1 mL/min;detection wavelength,246 nm;column temperature,30℃;and injection volume,10μL.[Results]The TLC chromatogram of F.pandurate Hance showed clear spots and good separation.The concentration of psoralen detected in the range of 2.06-41.20μg/mL had a good linear relationship with the peak area(r=0.9999).The RSD values of the precision,stability and reproducibility tests were all less than 2%.The average recovery rate was 100.2%(RSD=1.13%,n=6).[Conclusions]The established method is simple,easy,accurate and reproducible.It can be used for quality control of F.pandurate Hance.展开更多
[Objectives]To establish the quality standard for Zijinbiao.[Methods]Microscopic identification,physical and chemical identification,and thin-layer chromatography(TLC)were used to qualitatively identify Zijinbiao.The ...[Objectives]To establish the quality standard for Zijinbiao.[Methods]Microscopic identification,physical and chemical identification,and thin-layer chromatography(TLC)were used to qualitatively identify Zijinbiao.The moisture,total ash,acid-insoluble ash,and alcohol-soluble extract content were determined.The content of Plumbagin was determined by high performance liquid chromatography(HPLC).[Results]Microscopic identification,physical and chemical identification and thin layer identification features were remarkable.The moisture,total ash,acid-insoluble ash,and extract content of the 15 batches of samples were 7.49%-11.84%,2.43%-5.81%,0.59%-3.18%and 13.80%-20.45%,respectively.The linear equation of Plumbagin was Y=38094X,R^(2)=0.9996.Plumbagin had a good linear relationship in the range of 0.01-0.53 mg/mL.[Conclusions]This method is specific and reproducible,and can be used for quality control of Zijinbiao.展开更多
[Objectives]The research aimed to establish qualitative and quantitative detection methods of Haleniae Herba,and provide basis for improving its quality standard.[Methods]Using thin-layer chromatography(TLC),qualitati...[Objectives]The research aimed to establish qualitative and quantitative detection methods of Haleniae Herba,and provide basis for improving its quality standard.[Methods]Using thin-layer chromatography(TLC),qualitative identification of Haleniae Herba was conducted.Using high-performance liquid chromatography,luteolin content of Haleniae Herba was determined.[Results]TLC identification method had good specificity,with clear spots and good degree of separation,and reference medicinal material of Haleniae Herba was prepared.Content determination results displayed that luteolin had good linear relationship in the range of 0.088-1.760μg.The RSD values of precision,repeatability,stability and sample recovery of the instrument met the requirements.[Conclusions]The established method is simple and reproducible,and can be used for the quality control of Haleniae Herba.展开更多
基金Supported by National Key Technology Research and Development Program(2015BAC05B02)Fundamental Research Funds for the Central Universities(2018NZD10)
文摘[Objectives] The aim was to determine the moisture,ash and extract contents in Leontopodium franchetii Beauv. from different localities. [Methods] The contents of moisture,total ash,acid-insoluble ash and extract in L. franchetii from different localities were determined according to the methods described in Chinese Pharmacopoeia( 2015 Edition). [Results] In the 10 samples,the contents of moisture were all less than or equal to 15. 0%; the contents of total ash were all below 12. 0%; and the contents of acid-insoluble ash were all less than 3. 0%. The contents of water-soluble extract( by cold-soaked extraction method) in the samples were all below 12. 0% except those from Hongyuan Prairie of Sichuan Province and Xinglong Mountain in Lanzhou City,Gansu Province. [Conclusions]The contents of the four indicators measured in this experiment varied by small margins,indicating that the quality was relatively stable. This study provides theoretical data for the revision of the quality standards for L. franchetii.
基金the Research Committee of The Hong Kong Polytechnic University(No.G-V877)the Area of Excellence(AoE)of"Chinese Medicine Research and Further Development"of the University Grant Council(UGC)of Hong Kong Special Administrative Region(No.AoE/B-10/01)
文摘Nucleoside is the main class of active components in Cordyceps sinensis. Thin-layer chromatography (TLC) is one of the most commonly used methods in pharmacopoeias for analyzing chemical components of herbal medicine. Since the isocratic elution method cannot be applied successfully in TLC analysis for separating all the nucleoside components, the stepwise gradient elution has been developed in this work to separate eight nucleoside standards with success. In this way, quantitative analyses of the samples of Cordyceps sinensis were achieved via the pro-posed TLC procedure coupled with the scanning densitometric techniques of CAMAG and TLCQA methods for qualitative and quantitative analysis.
基金National Key R&D Program of China(Nos.2019YFC1711000 and 2018YFC1707900)the National Natural Science Foundation of China(No.8200140730)Qi-Huang Scholar of National Traditional Chinese Medicine Leading Talents Support Program(2018)。
文摘In this study,a high performance thin-layer chromatography/single quadrupole mass spectrometry QDa(HPTLC-QDa)method for robust authentication of Ganoderma lucidum,a popular and valuable herbal medicine,has been developed.This method is simple and practical,which allows direct generation of characteristic mass spectra from the HPTLC plates automatically with the application of in situ solvent desorption interface.The HPTLC silica gel plates were developed with toluene-ethyl formate-formic acid(5:5:0.2,V/V)and all bands were transferred to QDa system directly in situ using 80%methanol with 0.1%formic acid as desorption solvent.The acquired HPTLC-QDa spectra showed that luminous yellow band b3,containing ganoderic acid B/G/H and ganodeneric acid B,the major active components of Ganoderma,could be found only in G.lucidum and G.lucidum(Antler-shaped),but not in G.sinense and G.applanatum.Moreover,bands b13 and b14 with m/z 475/477 and m/z 475/491/495,respectively,could be detected in G.lucidum(Antler-shaped),but not in G.lucidum,thus allowing simple and robust authentication of G.lucidum with confused species.This method is proved to be simple,practical and reproducible,which can be extended to analyze other herbal medicines.
基金supported by the National Key Research and Development Program of China(No.2018YFC1707904,2018YFC1707900)。
文摘Objective:Belamcandae Rhizoma and Iridis Tectori Rhizoma are easily confused with each other.The main objective of this study is to distinguish them using chemical analysis.Materials and Methods:Thin-layer chromatography(TLC)and high-performance liquid chromatography(HPLC)fingerprint methods were established to compare the chemical profile,while HPLC quantitation was used to determine the contents of three isoflavones in thirty batches of Belamcandae Rhizoma and Iridis Tectori Rhizoma samples.Results:The two herbs could be distinguished by TLC using acetic acid-n hexane-ethyl acetate(1:90:80 v/v/v)as the mobile phase,according to the fluorescent band under 366 nm at R_(f) 0.2.In total,12 compounds were identified in the 24-min HPLC fingerprint.The similarity coefficient between the two herbs was 0.54±0.01.Mangiferin(1),tectoridin(2),iridin(3),irigenin(5),irisflorentin(6),and iristectorin A(9)were the main peaks in Belamcandae Rhizoma,while tectoridin(2)and tectorigenin(4)were the major peaks in Iridis Tectori Rhizoma.The contents of 2 in Iridis Tectori Rhizoma(2.50±0.20%)were 8.93 times higher than that of Belamcandae Rhizoma(0.28±0.08%),while the ones of 5 and 6 were slightly lower in Iridis Tectori Rhizoma.Conclusions:The study established fast and effective methods to distinguish Belamcandae Rhizoma from Iridis Tectori Rhizoma.
基金financially supported by National Natural Science Foundation of China (21804058)Shanxi Postdoc Reward (SXBYKY2022001)+1 种基金Shanxi Scholarship Council of China (2021068)Shanxi Agricultural University High-Level Talent Project (2021XG013)。
文摘As a widely used food preservative,methyl paraben was experimentally evidenced with serious hormonelike adverse effects.Herein,a high performance thin-layer chromatography platformed bioluminescent bioautography and image analysis for the selective quantification and confirmation of methyl paraben was proposed and validated in vinegar and coconut juice.First,the detectability of the bioautography to the analyte on different layer materials was estimated,revealing that normal silica gel was the best choice.After that,the liquid of sample extract and working solution were separated to overcome the background noises due to co-extracted matrices.The separation result was then coupled to the optimized bioautography,enabling instant and straightforward screening of the targeted conpound.For accurate quantification,bioluninescent inhibition pattern caused by the analyte was processed by image analysis,giving useful sensitivity(LOD>16 mg/kg),precision(RSD<10.1%)and accuracy(spike-recovery rate 76.9%-112.2%).Finally,the suspected result was confirmed by determining its MS fingerprint,further strengthening the reliability of screening.
基金Supported by the Office of the Vice-Chancellor for Research and Development for the Outright Research Grant(Project No.151516 PNSE),the University of the Philippines Diliman
文摘Objective: To determine the thin-layer chromatography(TLC) fingerprint profiles and to evaluate the in vitro antioxidant activity of ethanol extracts of Muntingia calabura(M. calabura) leaves and stems.Methods: The leaves and stems were extracted using ethanol as solvent. The TLC separation of the phytochemical constituents of the leaf and ethanol extracts was carried out in ethyl acetate: n-hexane and chloroform: ethyl acetate mobile phase systems.Distinct spots were visualized under visible light, UV 254 nm, UV 366 nm and after spraying with vanillin-sulfuric acid. The 2,2-diphenyl-1-picrylhydrazyl free-radical scavenging assay was used to evaluate the antioxidant activity of the extracts.Results: Both the leaf and stem ethanol extracts at 4 mg/mL exhibited 2,2-diphenyl-1-picrylhydrazyl inhibition of more than 90%, relative to gallic acid. The results of TLC showed that the degree of resolution between the constituent spots was comparable between the two mobile phase systems using the different visualization wavelengths. Under the 254 nm visualization, few spots were observed in leaf and stem extracts. Visualization at 366 nm yielded the greatest number of observable spots of various colors in both leaf and stem extracts. More spots were visualized upon post-derivatization with vanillinsulfuric acid in the TLC chromatograms using chloroform: ethyl acetate mobile phase,compared to those in ethyl acetate: n-hexane mobile phase.Conclusions: M. calabura exhibited very high antioxidant activity in its leaves and stems ethanol extracts, both of which are used in traditional medicine. The TLC results demonstrated the presence of diverse secondary metabolites in the leaf and stem ethanol extracts, indicating that the antioxidant activity, including other bioactivities may be attributed to these phytochemical constituents. This paper has reported for the first time the TLC fingerprinting of M. calabura using visible light, UV 254 nm, UV 366 and postderivatization with vanillin-spray to visualize separate spots on TLC plates.
文摘Aflatoxin B1 is a mycotoxin that can contaminate a wide feedstuffs variety. Ingestion of contaminated feed by poultry can lead to impaired health and zootechnical performances but also a human diet safety problem related to residues presence in animal origin products. Aflatoxin B1 contamination of poultry feed samples marketed in Dakar city and in peri-urban areas (Gorom, Sangalkam) was studied. A total of 15 samples were collected from Dakar city markets as well as from poultry farms in Gorom and Sangalkam areas. Aflatoxin B1 quantification was performed by high performance liquid chromatography and thin-layer chromatography. HPLC results showed that all samples were contaminated with levels ranging from 0.15 to 22 ppb, 0.099 to 2.05 ppb and 0.099 to 4.95 ppb respectively for Gorom, Sangalkam and Dakar. Only the finishing feed from Gorom had an aflatoxin B1 level above the maximum limit set by regulations. TLC is a suitable method for aflatoxins detection. However, it was associated with overestimation for aflatoxin B1 quantification. Results suggest that poultry feed represents a real source of human diet contamination. In addition, HPLC remains the most reliable quantification technique for quality control.
基金National Characteristic TCM Technology Inheritance Talent Fostering Program(No.20184828005)Project for Enhancing Basic Scientific Research Ability of Young and Middle-aged Teachers in Colleges and Universities of Guangxi(No.2019KY0341)+1 种基金Natural Science Foundation of Guangxi(No.2020GXNSFAA259059)Prescription Development Project of the First Affiliated Hospital of Guangxi University of Chinese Medicine(No.2017ZJ001).
文摘[Objectives]To establish a method for caffeic acid content determination and thin-layer chromatography identification of the Zhuang medicine Argyreia acuta Lour.[Methods]Silica gel GF254(thin-layer plate),toluene-formic acid-ethyl acetate(V∶V∶V=5∶1.2∶3)(developing agent)and 365 nm ultraviolet light were used for thin-layer chromatography identification.Under the chromatographic conditions of column of Thermo SCIENTIFIC Hypersil C18(5μm,4.60 mm×250 mm),mobile phase of methanol-0.1%phosphoric acid(23∶77),detection wavelength of 320 nm,column temperature of 30℃,sample size of 10μL and flow rate of 1.0 mL/min,the content of caffeic acid in A.acuta Lour was determined.[Results]Caffeic acid can be detected in thin layer chromatography,with strong specificity and clear spots.When the sample size of caffeic acid is within the range of 0.0151-0.4530μg(R^(2)=0.9999),the content of caffeic acid showed a relatively good linear relationship with the peak area.The average recovery rate of A.acuta Lour was 99.94%(RSD=2.68%),97.56%(RSD=1.57%)and 99.79%(RSD=2.05%),respectively.[Conclusions]This method can effectively identify A.acuta Lour,and can accurately determine the content of caffeic acid in A.acuta Lour.It has characteristics of high accuracy,high precision,good color rendering stability,good reproducibility and fast analysis speed.
文摘[Objectives]This study aimed to establish a qualitative identification and content determination method for psoralen in Ficus pandurata Hance and compare the psoralen contents in roots,stems and leaves of F.pandurate Hance and Ficus pandurata Hance var.holophylla Migo.[Methods]Thin-layer chromatography(TLC)was used for qualitative identification,and the content of psoralen was determined by high-performance liquid chromatography.The chromatographic conditions were as follows:column,Agilent ZORBAX Eclipse Plus C18(250 mm×4.6 mm,5μm);mobile phase,methanol-water(55∶45);flow rate,1 mL/min;detection wavelength,246 nm;column temperature,30℃;and injection volume,10μL.[Results]The TLC chromatogram of F.pandurate Hance showed clear spots and good separation.The concentration of psoralen detected in the range of 2.06-41.20μg/mL had a good linear relationship with the peak area(r=0.9999).The RSD values of the precision,stability and reproducibility tests were all less than 2%.The average recovery rate was 100.2%(RSD=1.13%,n=6).[Conclusions]The established method is simple,easy,accurate and reproducible.It can be used for quality control of F.pandurate Hance.
基金Supported by National Key R&D Program(2018YFC1706101)Sichuan Provincial Key R&D Project(2021YFS0043)+2 种基金Chinese Medicine(Ethnic Medicine)Standard Improvement Project of Sichuan Medical Products Administration(510201202102305)Leading Talent Support Program of National Ethnic Affairs Commission of the People’s Republic of China in 2021School Level Innovation Team of Fundamental Research Funds for the Central Universities(ZYN2022067)。
文摘[Objectives]To establish the quality standard for Zijinbiao.[Methods]Microscopic identification,physical and chemical identification,and thin-layer chromatography(TLC)were used to qualitatively identify Zijinbiao.The moisture,total ash,acid-insoluble ash,and alcohol-soluble extract content were determined.The content of Plumbagin was determined by high performance liquid chromatography(HPLC).[Results]Microscopic identification,physical and chemical identification and thin layer identification features were remarkable.The moisture,total ash,acid-insoluble ash,and extract content of the 15 batches of samples were 7.49%-11.84%,2.43%-5.81%,0.59%-3.18%and 13.80%-20.45%,respectively.The linear equation of Plumbagin was Y=38094X,R^(2)=0.9996.Plumbagin had a good linear relationship in the range of 0.01-0.53 mg/mL.[Conclusions]This method is specific and reproducible,and can be used for quality control of Zijinbiao.
文摘[Objectives]The research aimed to establish qualitative and quantitative detection methods of Haleniae Herba,and provide basis for improving its quality standard.[Methods]Using thin-layer chromatography(TLC),qualitative identification of Haleniae Herba was conducted.Using high-performance liquid chromatography,luteolin content of Haleniae Herba was determined.[Results]TLC identification method had good specificity,with clear spots and good degree of separation,and reference medicinal material of Haleniae Herba was prepared.Content determination results displayed that luteolin had good linear relationship in the range of 0.088-1.760μg.The RSD values of precision,repeatability,stability and sample recovery of the instrument met the requirements.[Conclusions]The established method is simple and reproducible,and can be used for the quality control of Haleniae Herba.
