[ Objective ] The paper was to study the growth-promoting and antagonistic action of endophytic bacteria strains Itb57 and Itb295 of tobacco to explore their functions in biological control. [ Method] The growth-promo...[ Objective ] The paper was to study the growth-promoting and antagonistic action of endophytic bacteria strains Itb57 and Itb295 of tobacco to explore their functions in biological control. [ Method] The growth-promoting effects of bacterial suspension ~ff endophytic bacteria Itb57 and Itb295 on tobacco seedling un- der different treatment modes were studied using potting method in greenhouse. The antagonistic action of bacterial suspension of endophytic bacteria Itb57 and Itb295 on Phytophthora nicotianae, Alternaria alternata and Botrytis cinerea were measured by duel culture method. [ Result] Bacterial suspensions of enduphytic bacteria Itb57 and Itb295 had certain growth-promoting effects on tobacco seedling, which could significantly increase the fresh weight and dry weight in aerial part; the growth-promoting effect of soaking ＋ spraying and irrigating treatment was the best. Itb57 strain had good antagonistic action against P. nicotianae. A. alterna- ta and B. cinerea, while Itb295 strain only had good antifungal effect against P. nicotianae. [ Conclusion] The results provided basis for the study and application of tobacco endophytic bacteria strains Itb57 and Itb295 in biocontrol of tobacco diseases.展开更多
The growth of flue-cured tobacco can be significantly influenced by the temperature, sunlight, water, etc. The paper presents and analyzes the details of the disease influence on the flue-cured tobacco during the grow...The growth of flue-cured tobacco can be significantly influenced by the temperature, sunlight, water, etc. The paper presents and analyzes the details of the disease influence on the flue-cured tobacco during the growth and development period in the high-mountainous and the middle-mountainous regions of Enshi based on the detailed climatolgical data. The results demonstrate that the most important climatic factors affecting the growth and development of flue-cured tobacco contain low temperature, even rainy, lack of sunlight and low evaporation. The deterious ef-fects in the high-mountainous region, the heavy fog region and the flat ground are more serious compared with the middle- and low-mountainous regions, the light fog region and the sloping field, respectively. Furthermore, in order to reduce the deteri-ous effects, helpful countermeasures are proposed favoring the growth and produc-tion of the flue-cured tobacco.展开更多
为缓解氯胁迫对烟苗生长发育的不利影响,本试验以水凝胶为材料,设置5个处理即CK(不施KCl和水凝胶)、CK1(1.15 g KCl/盆+0 g水凝胶)、T1(1.15 g KCl/盆+1 g水凝胶/盆)、T2(1.15 g KCl/盆+3 g水凝胶/盆)和T3(1.15 g KCl/盆+5 g水凝胶/盆)...为缓解氯胁迫对烟苗生长发育的不利影响,本试验以水凝胶为材料,设置5个处理即CK(不施KCl和水凝胶)、CK1(1.15 g KCl/盆+0 g水凝胶)、T1(1.15 g KCl/盆+1 g水凝胶/盆)、T2(1.15 g KCl/盆+3 g水凝胶/盆)和T3(1.15 g KCl/盆+5 g水凝胶/盆),研究氯胁迫下施用水凝胶对烟苗生长发育、抗氧化系统和常规化学成分的影响。结果表明,随着水凝胶用量增加,烟苗氯含量呈下降趋势,T2、T3处理烟苗氯含量较CK1分别降低21.35%和30.62%;T2处理烟苗地上部地下部鲜重、地上部地下部干重较CK1分别提高16.90%、20.29%、20.00%和30.93%,T3处理烟苗地上部地下部鲜重、地上部地下部干重较CK1分别提高27.70%、22.46%、30.77%和37.11%;T2、T3处理烟苗光合速率较CK1分别提高29.23%、41.78%;T2处理烟苗过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性以及丙二醛(MDA)含量较CK1分别降低19.30%、22.11%、12.98%和13.25%,T3处理烟苗CAT、POD、SOD活性以及MDA含量较CK1分别降低19.91%、21.59%、21.13%和20.33%;T2、T3处理有效提高氯胁迫下烟苗烟碱、总糖、还原糖含量和钾氯比。综上可见,施用水凝胶可有效缓解氯胁迫对烟苗的不利影响,促进烟苗生长发育。展开更多
基金Supported by Natural Science Foundation of Chongqing City(CSTC,2009BB1294)~~
文摘[ Objective ] The paper was to study the growth-promoting and antagonistic action of endophytic bacteria strains Itb57 and Itb295 of tobacco to explore their functions in biological control. [ Method] The growth-promoting effects of bacterial suspension ~ff endophytic bacteria Itb57 and Itb295 on tobacco seedling un- der different treatment modes were studied using potting method in greenhouse. The antagonistic action of bacterial suspension of endophytic bacteria Itb57 and Itb295 on Phytophthora nicotianae, Alternaria alternata and Botrytis cinerea were measured by duel culture method. [ Result] Bacterial suspensions of enduphytic bacteria Itb57 and Itb295 had certain growth-promoting effects on tobacco seedling, which could significantly increase the fresh weight and dry weight in aerial part; the growth-promoting effect of soaking ＋ spraying and irrigating treatment was the best. Itb57 strain had good antagonistic action against P. nicotianae. A. alterna- ta and B. cinerea, while Itb295 strain only had good antifungal effect against P. nicotianae. [ Conclusion] The results provided basis for the study and application of tobacco endophytic bacteria strains Itb57 and Itb295 in biocontrol of tobacco diseases.
基金Supported by the Science and Technology Development Fund of Hubei Weather Bureau(Grant No.2013Y06)~~
文摘The growth of flue-cured tobacco can be significantly influenced by the temperature, sunlight, water, etc. The paper presents and analyzes the details of the disease influence on the flue-cured tobacco during the growth and development period in the high-mountainous and the middle-mountainous regions of Enshi based on the detailed climatolgical data. The results demonstrate that the most important climatic factors affecting the growth and development of flue-cured tobacco contain low temperature, even rainy, lack of sunlight and low evaporation. The deterious ef-fects in the high-mountainous region, the heavy fog region and the flat ground are more serious compared with the middle- and low-mountainous regions, the light fog region and the sloping field, respectively. Furthermore, in order to reduce the deteri-ous effects, helpful countermeasures are proposed favoring the growth and produc-tion of the flue-cured tobacco.
文摘为缓解氯胁迫对烟苗生长发育的不利影响,本试验以水凝胶为材料,设置5个处理即CK(不施KCl和水凝胶)、CK1(1.15 g KCl/盆+0 g水凝胶)、T1(1.15 g KCl/盆+1 g水凝胶/盆)、T2(1.15 g KCl/盆+3 g水凝胶/盆)和T3(1.15 g KCl/盆+5 g水凝胶/盆),研究氯胁迫下施用水凝胶对烟苗生长发育、抗氧化系统和常规化学成分的影响。结果表明,随着水凝胶用量增加,烟苗氯含量呈下降趋势,T2、T3处理烟苗氯含量较CK1分别降低21.35%和30.62%;T2处理烟苗地上部地下部鲜重、地上部地下部干重较CK1分别提高16.90%、20.29%、20.00%和30.93%,T3处理烟苗地上部地下部鲜重、地上部地下部干重较CK1分别提高27.70%、22.46%、30.77%和37.11%;T2、T3处理烟苗光合速率较CK1分别提高29.23%、41.78%;T2处理烟苗过氧化氢酶(CAT)、过氧化物酶(POD)、超氧化物歧化酶(SOD)活性以及丙二醛(MDA)含量较CK1分别降低19.30%、22.11%、12.98%和13.25%,T3处理烟苗CAT、POD、SOD活性以及MDA含量较CK1分别降低19.91%、21.59%、21.13%和20.33%;T2、T3处理有效提高氯胁迫下烟苗烟碱、总糖、还原糖含量和钾氯比。综上可见,施用水凝胶可有效缓解氯胁迫对烟苗的不利影响,促进烟苗生长发育。
