Ray tracing is a computer graphics method that renders images realistically. As the name suggests, this technique primarily traces the path of light rays interacting with objects in a scene [1], permitting the calcula...Ray tracing is a computer graphics method that renders images realistically. As the name suggests, this technique primarily traces the path of light rays interacting with objects in a scene [1], permitting the calculation of lighting and reflecting impact [2]. As ray tracing is a time-consuming process, the need for parallelization to solve this problem arises. One downside of this solution is the existence of race conditions. In this work, we explore and experiment with a different, well-known solution for this race condition. Starting with the introduction and the background section, a brief overview of the topic is followed by a detailed part of how the race conditions may occur in the case of the ray tracing algorithm. Continuing with the methods and results section, we have used OpenMP to parallelize the Ray tracing algorithm with the different compiler directives critical, atomic, and first-private. Hence, it concluded that both critical and atomic are not efficient solutions to produce a good-quality picture, but first-private succeeded in producing a high-quality picture.展开更多
Analyzing the structure and function of the brain's neural network is critical for identifying the working principles of the brain and the mechanisms of brain diseases.Recombinant rabies viral vectors allow for th...Analyzing the structure and function of the brain's neural network is critical for identifying the working principles of the brain and the mechanisms of brain diseases.Recombinant rabies viral vectors allow for the retrograde labeling of projection neurons and cell type-specific trans-monosynaptic tracing,making these vectors powerful candidates for the dissection of synaptic inputs.Although several attenuated rabies viral vectors have been developed,their application in studies of functional networks is hindered by the long preparation cycle and low yield of these vectors.To overcome these limitations,we developed an improved production system for the rapid rescue and preparation of a high-titer CVS-N2c-ΔG virus.Our results showed that the new CVS-N2c-ΔG-based toolkit performed remarkably:(1)N2cG-coated CVS-N2c-ΔG allowed for efficient retrograde access to projection neurons that were unaddressed by rAAV9-Retro,and the efficiency was six times higher than that of rAAV9-Retro;(2)the trans-monosynaptic efficiency of oG-mediated CVS-N2c-ΔG was 2–3 times higher than that of oG-mediated SAD-B19-ΔG;(3)CVS-N2c-ΔG could delivery modified genes for neural activity monitoring,and the time window during which this was maintained was 3 weeks;and(4)CVS-N2c-ΔG could express sufficient recombinases for efficient transgene recombination.These findings demonstrate that new CVS-N2c-ΔG-based toolkit may serve as a versatile tool for structural and functional studies of neural circuits.展开更多
Successful establishment of reconnection between retinal ganglion cells and retinorecipient regions in the brain is critical to optic nerve regeneration.However,morphological assessments of retinorecipient regions are...Successful establishment of reconnection between retinal ganglion cells and retinorecipient regions in the brain is critical to optic nerve regeneration.However,morphological assessments of retinorecipient regions are limited by the opacity of brain tissue.In this study,we used an innovative tissue cleaning technique combined with retrograde trans-synaptic viral tracing to observe changes in retinorecipient regions connected to retinal ganglion cells in mice after optic nerve injury.Specifically,we performed light-sheet imaging of whole brain tissue after a clearing process.We found that pseudorabies virus 724(PRV724)mostly infected retinal ganglion cells,and that we could use it to retrogradely trace the retinorecipient regions in whole tissue-cleared brains.Unexpectedly,PRV724-traced neurons were more widely distributed compared with data from previous studies.We found that optic nerve injury could selectively modify projections from retinal ganglion cells in the hypothalamic paraventricular nucleus,intergeniculate leaflet,ventral lateral geniculate nucleus,central amygdala,basolateral amygdala,Edinger-Westphal nucleus,and oculomotor nucleus,but not the superior vestibular nucleus,red nucleus,locus coeruleus,gigantocellular reticular nucleus,or facial nerve nucleus.Our findings demonstrate that the tissue clearing technique,combined with retrograde trans-synaptic viral tracing,can be used to objectively and comprehensively evaluate changes in mouse retinorecipient regions that receive projections from retinal ganglion cells after optic nerve injury.Thus,our approach may be useful for future estimations of optic nerve injury and regeneration.展开更多
Submarine groundwater discharge(SGD)is an important part in the land-sea interactions,which mainly contains three components:submarine fresh groundwater discharge(SFGD),tidal flat recirculated saline groundwater disch...Submarine groundwater discharge(SGD)is an important part in the land-sea interactions,which mainly contains three components:submarine fresh groundwater discharge(SFGD),tidal flat recirculated saline groundwater discharge(tidal flat RSGD)and subtidal recirculated saline groundwater discharge(subtidal RSGD).In order to make a more accurate assessment of the impact of SGD on coastal ecological environment,it is necessary to distinguish the main components of SGD.In this study,the Maowei Sea,located in the northern part of the Beibu Gulf,was selected as the study area.Based on the radium(Ra)tracing method,we present a new analytical method for distinguishing the three main components of SGD in this area combined with field data.The average daily flow along the coastline of the Maowei Sea of tidal flat RSGD was slightly higher than that of SFGD,and both two were on the magnitude of 1×10^(5)m^(3)/d.The average daily flow for the subtidal RSGD of the entire subtidal zone of the Maowei Sea reached to the magnitude of 1×10^(6)-1×10^(7)m^(3)/d.The long-term variation trend of terrestrial SGD is a valuable information for the study of the influence of terrigenous material on the coastal ecological environment.Based on the results of four sampling periods,it is found that the fluxes of SFGD and tidal flat RSGD in the Maowei Sea had good linear correlation with the net precipitation.As an example,January 2015 to August 2022 were selected as the study periods,and the variation trends of SFGD and tidal flat RSGD were calculated by linear function with net precipitation as the independent variable.The results showed that the flux of tidal flat RSGD was slightly higher than that of SFGD,and the difference between the two is larger in flood season while smaller in dry season.In general,in the coastal range of China,the total SGD flux in the Maowei Sea area is at a high level,and the SFGD flux is at a medium level.展开更多
With the popularity of online learning in educational settings, knowledge tracing(KT) plays an increasingly significant role. The task of KT is to help students learn more effectively by predicting their next mastery ...With the popularity of online learning in educational settings, knowledge tracing(KT) plays an increasingly significant role. The task of KT is to help students learn more effectively by predicting their next mastery of knowledge based on their historical exercise sequences. Nowadays, many related works have emerged in this field, such as Bayesian knowledge tracing and deep knowledge tracing methods. Despite the progress that has been made in KT, existing techniques still have the following limitations: 1) Previous studies address KT by only exploring the observational sparsity data distribution, and the counterfactual data distribution has been largely ignored. 2) Current works designed for KT only consider either the entity relationships between questions and concepts, or the relations between two concepts, and none of them investigates the relations among students, questions, and concepts, simultaneously, leading to inaccurate student modeling. To address the above limitations,we propose a graph counterfactual augmentation method for knowledge tracing. Concretely, to consider the multiple relationships among different entities, we first uniform students, questions, and concepts in graphs, and then leverage a heterogeneous graph convolutional network to conduct representation learning.To model the counterfactual world, we conduct counterfactual transformations on students’ learning graphs by changing the corresponding treatments and then exploit the counterfactual outcomes in a contrastive learning framework. We conduct extensive experiments on three real-world datasets, and the experimental results demonstrate the superiority of our proposed Graph CA method compared with several state-of-the-art baselines.展开更多
Parametric curves such as Bézier and B-splines, originally developedfor the design of automobile bodies, are now also used in image processing andcomputer vision. For example, reconstructing an object shape in an...Parametric curves such as Bézier and B-splines, originally developedfor the design of automobile bodies, are now also used in image processing andcomputer vision. For example, reconstructing an object shape in an image,including different translations, scales, and orientations, can be performedusing these parametric curves. For this, Bézier and B-spline curves can be generatedusing a point set that belongs to the outer boundary of the object. Theresulting object shape can be used in computer vision fields, such as searchingand segmentation methods and training machine learning algorithms. Theprerequisite for reconstructing the shape with parametric curves is to obtainsequentially the points in the point set. In this study, a novel algorithm hasbeen developed that sequentially obtains the pixel locations constituting theouter boundary of the object. The proposed algorithm, unlike the methods inthe literature, is implemented using a filter containing weights and an outercircle surrounding the object. In a binary format image, the starting point ofthe tracing is determined using the outer circle, and the next tracing movementand the pixel to be labeled as the boundary point is found by the filter weights.Then, control points that define the curve shape are selected by reducing thenumber of sequential points. Thus, the Bézier and B-spline curve equationsdescribing the shape are obtained using these points. In addition, differenttranslations, scales, and rotations of the object shape are easily provided bychanging the positions of the control points. It has also been shown that themissing part of the object can be completed thanks to the parametric curves.展开更多
A method was proposed to analyze the influences of the non-ideal spectroscopic performance of optical components and orientation errors of a laser tracing measurement optical system on the tracing measurement performa...A method was proposed to analyze the influences of the non-ideal spectroscopic performance of optical components and orientation errors of a laser tracing measurement optical system on the tracing measurement performance.A comprehensive model of the interference fringe contrast based on the laser tracing system s measurement principle was established in this study.Simulation results based on ZEMAX verified the model.According to the simulation results,the placement angle of the analyzer had a direct influence on the interference fringe contrast.When the angle of the polarized light to the analyzer’s transmission axis increased from 65°to 85°,each contrast of the four-way interference fringes decreased from 0.9996 to 0.3528,the interference fringe contrast is decreased by 65%.Under the split ratio of beam splitters in the interference part(BS 1)of 5∶5,when the splitting ratio of BS 2 changed from 2∶8 to 8∶2,the fringe contrast of the interference signals received by the photodetectors increased,but the injection light intensity onto the PSD reflected by BS 2 decreased.The significant influence of the tracing performance was verified by the experiments.When splitting ratio of BS 2 increased,the contrast of the interference fringes increased.Due to the weakening of the incident light intensity of the PSD caused by the change of BS 2 splitting ratio,the response time of the tracing system is increased by 23.7 ms.As a result,the tracing performance of the laser tracing measurement optical system was degraded.An important theoretical basis was provided to evaluate and improve the accuracy and reliability of laser tracing measurement systems.展开更多
Radio propagation environment plays a critical role in the performance of wireless communication systems,and understanding channel characteristics is vital for ensuring reliable communication links and optimizing syst...Radio propagation environment plays a critical role in the performance of wireless communication systems,and understanding channel characteristics is vital for ensuring reliable communication links and optimizing system performance.Ray tracing is an effective method to investigate propagation characteristics in a complex environment,and how to quickly and accurately obtain environmental information needs to be solved.This paper presents dynamic environment reconstruction and ray tracing simulation in railway tunnel environment based on Simultaneous Localization and Mapping(SLAM)algorithm and Poisson reconstruction algorithm.Accurate channel parameters are obtained and analyzed based on ray tracing simulation.Both straight and curved tunnels are considered and investigated,and the results show the channel characteristics in complex railway tunnel environments.展开更多
Regenerating functional new neurons in the adult mammalian central nervous system has been proven to be very challenging due to the inability of neurons to divide and repopulate themselves after neuronal loss.Glial ce...Regenerating functional new neurons in the adult mammalian central nervous system has been proven to be very challenging due to the inability of neurons to divide and repopulate themselves after neuronal loss.Glial cells,on the other hand,can divide and repopulate themselves under injury or diseased conditions.We have previously reported that ectopic expression of NeuroD1 in dividing glial cells can directly convert them into neurons.Here,using astrocytic lineage-tracing reporter mice(Aldh1l1-CreERT2 mice crossing with Ai14 mice),we demonstrate that lineage-traced astrocytes can be successfully converted into NeuNpositive neurons after expressing NeuroD1 through adeno-associated viruses.Retroviral expression of NeuroD1 further confirms that dividing glial cells can be converted into neurons.Importantly,we demonstrate that for in vivo cell conversion study,using a safe level of adeno-associated virus dosage(10^10–10^12 gc/mL,1μL)in the rodent brain is critical to avoid artifacts caused by toxic dosage,such as that used in a recent bioRxiv study(2×10^13 gc/mL,1μL,mouse cortex).For therapeutic purpose under injury or diseased conditions,or for non-human primate studies,adeno-associated virus dosage needs to be optimized through a series of dose-finding experiments.Moreover,for future in vivo gliato-neuron conversion studies,we recommend that the adeno-associated virus results are further verified with retroviruses that mainly express transgenes in dividing glial cells in order to draw solid conclusions.The study was approved by the Laboratory Animal Ethics Committee of Jinan University,China(approval No.IACUC-20180330-06)on March 30,2018.展开更多
A new method of Neutron Activation Analysis (NAA) for sediment movement study is presented in this paper. Through analyzing the natural sediment, the information about natural tracing agents has been obtained, which c...A new method of Neutron Activation Analysis (NAA) for sediment movement study is presented in this paper. Through analyzing the natural sediment, the information about natural tracing agents has been obtained, which can show the direction of sediment movement and relative discharge of sediment transportation. Test results in some areas, such as Shantou Harbour and Hangzhou Bay, are in good agreement with those from normal hydrologic measurements and some results are not obtainable with the latter. The NAA is economical, practical and reliable, and should be widely used in engineering practice.展开更多
In 5G era,it is expected to achieve wireless network coverage including offshore areas.Modeling of marine wireless channels is the basis of constructing a marine communication system.In this paper,a communication scen...In 5G era,it is expected to achieve wireless network coverage including offshore areas.Modeling of marine wireless channels is the basis of constructing a marine communication system.In this paper,a communication scene between an unmanned aerial vehicle(UAV)and a boat is simulated to study the marine wireless channel.Firstly,an improved spatial partitioning ray tracing algorithm is proposed to track the propagation path of electromagnetic waves at sea surface.Secondly,a mobile channel is simulated and modeled based on the track results.Finally,a loss measurement is carried out in the coastal waters based on the simple wireless channel loss measuring platform,and a path loss propagation model is built.Then we compare the actual measurement data with the simulation results and find that the two are have good consistency,which further verifies the reliability of the simulation.展开更多
A new algorithm for ray tracing bicubic Bezier surface intersection is presented. In order to find the intersected patches more effectively, a quadtree representation for surface patches is utilized. The introduction ...A new algorithm for ray tracing bicubic Bezier surface intersection is presented. In order to find the intersected patches more effectively, a quadtree representation for surface patches is utilized. The introduction of the alternative binary tree subdivision of Bezier surface effectively improves the performance of the ray surface intersection, with the benefit of avoiding the problem that the binary tree subdivision would produce long narrow patches and speeding the intersection finding process. The algorithm has been programmed in FORTRAN-77 and on prime-550 II computer. The result shows that its structure is simple and it is easy to implement with good performance.展开更多
The surface-related multiple elimination(SRME) method is based on feedback formulation and has become one of the most preferred multiple suppression methods used. However, some differences are apparent between the pre...The surface-related multiple elimination(SRME) method is based on feedback formulation and has become one of the most preferred multiple suppression methods used. However, some differences are apparent between the predicted multiples and those in the source seismic records, which may result in conventional adaptive multiple subtraction methods being barely able to effectively suppress multiples in actual production. This paper introduces a combined adaptive multiple attenuation method based on the optimized event tracing technique and extended Wiener filtering. The method firstly uses multiple records predicted by SRME to generate a multiple velocity spectrum, then separates the original record to an approximate primary record and an approximate multiple record by applying the optimized event tracing method and short-time window FK filtering method. After applying the extended Wiener filtering method, residual multiples in the approximate primary record can then be eliminated and the damaged primary can be restored from the approximate multiple record. This method combines the advantages of multiple elimination based on the optimized event tracing method and the extended Wiener filtering technique. It is an ideal method for suppressing typical hyperbolic and other types of multiples, with the advantage of minimizing damage of the primary. Synthetic and field data tests show that this method produces better multiple elimination results than the traditional multi-channel Wiener filter method and is more suitable for multiple elimination in complicated geological areas.展开更多
For large-scale 3D seismic data,target-oriented reservoir imaging is more attractive than conventional full-volume migration,in terms of computation efficiency.Gaussian beam migration(GBM)is one of the most robust dep...For large-scale 3D seismic data,target-oriented reservoir imaging is more attractive than conventional full-volume migration,in terms of computation efficiency.Gaussian beam migration(GBM)is one of the most robust depth imaging method,which not only keeps the advantages of ray methods,such as high efficiency and flexibility,but also allows us to solve caustics and multipathing problems.But conventional Gaussian beam migration requires slant stack for prestack data,and ray tracing from beam center location to subsurface,which is not easy to be directly applied for target-oriented imaging.In this paper,we modify the conventional Gaussian beam migration scheme,by shooting rays from subsurface image points to receivers to implement wavefield back-propagation.This modification helps us to achieve a better subsurface illumination in complex structure and allows simple implementation for target reservoir imaging.Significantly,compared with the wavefi eld-based GBM,our method does not reconstruct the subsurface snapshots,which has higher efficiency.But the proposed method is not as efficient as the conventional Gaussian beam migration.Synthetic and field data examples demonstrate the validity and the target-oriented imaging capability of our method.展开更多
Incorporation of the Monte Carlo(MC)algorithm in optimizing CyberKnife(CK)plans is cumbersome,and early models unconfigured MC calculations,therefore,this study investigated algorithm-based dose calculation discrepanc...Incorporation of the Monte Carlo(MC)algorithm in optimizing CyberKnife(CK)plans is cumbersome,and early models unconfigured MC calculations,therefore,this study investigated algorithm-based dose calculation discrepancies by selecting different prescription isodose lines(PIDLs)in head and lung CK plans.CK plans were based on anthropomorphic phantoms.Four shells were set at 2-60 mm from the target,and the constraint doses were adjusted according to the design stratcgy.After optimization,30%-90%PIDL plans were generated by ray tracing(RT).In the evaluation module,CK plans were recalculated using the MC algorithm.Therefore,the dosimetric parameters of different PIDL plans based on the RT and MC algorithms were obtained and analyzed.The discrepancies(mean+SD)were 3.72%+0.31%,3.40%+0.11%,3.47%+0.32%,0.17%+0.11%,0.64%+3.60%,7.73%+1.60%,14.62%+3.21%and 10.10%+1.57%for Djs,Dmeam),Dys,and coverage of the PTV,DGI,V,,V;and V,in the head plans and-6.32%+1.15%,-13.46%+0.98%,-20.63%+2.25%,-34.78%+25.03%,12248%+175.60%,-12.92%+5.41%,3.19%+4.67%and 7.13%+1.56%in the lung plans,respectively.The following parameters were significantly correlated with PIDL:dp98%at the 0.05 level and dpal,dys and dv3 at the 0.01 level for the head plans;dp98e%at the 0.05 level and do1e%,dpmeam,Ccoweange,dool,dvs and dv;at the 0.01 level for the lung plans.RT may be used to calculate the dose in CK head plans,but when the dose of organs at risk is close to the limit,it is necessary to refer to the MC results or to further optimize the CK plan to reduce the dose.For lung plans,the MC algorithm is recommended.For early models without the MC algorithm,a lower PIDL plan is recommended;otherwise,a large PIDL plan risks serious underdosage in the target area.展开更多
This paper deals with characteristics of silicon isotope compositions and siliceous cathodolumines-cence of host rocks, ores and hydrothermal silicified quartz of the Carlin-type ore deposits in the Yunnan-Guizhou-Gua...This paper deals with characteristics of silicon isotope compositions and siliceous cathodolumines-cence of host rocks, ores and hydrothermal silicified quartz of the Carlin-type ore deposits in the Yunnan-Guizhou-Guangxi triangle area. The study shows that primary silicified quartz is nonluminescent but quartz in host rocks andsecondary silicified quartz are luminescent by the action of cathode rays. Correspondingly, silicon isotope composi-tions of host rocks, ores and hydrothermal quartz veins are clearly distinguished. In strata from the Middle Triassic tothe 'Dachang' host bed, δ<sup>30</sup>Si of the host rocks ranges from 0.0‰-0.3‰, while that of primary ore-forming silici-fied fluids from -0.1‰ to -0.4‰, in the Upper Permian and Lower Carboniferous strata and Indosinian diabasehost beds, δ<sup>30</sup>Si of the host rocks is from -0.1‰ to -0.2‰ and that of the primary silicified quartz veins from 0.3‰-0.5‰. This pattern demonstrates the following geochemical mineralization process: primary ore-forming sili-ceous fluids migrated upwards quickly along the main passages of deep-seated faults from mantle to crust and en-tered secondary faults where gold deposits were eventually formed as a result of permeation and replacement of thesiliceous ore-forming fluids into different ore-bearing strata. This gives important evidence for the fact that ore-forming fluids of this type of gold deposits were mainly derived from upper mantle differentiation and shows goodprospects for deep gold deposits and geochemical background for large and superlarge gold deposits.展开更多
Three different types of gold and silver deposits in Zhejiang Province(Huangshan gold deposit, Zhilingtou gold-silver deposit and Haoshi silver deposit) showmarked differences in lead and strontium isotopic compositio...Three different types of gold and silver deposits in Zhejiang Province(Huangshan gold deposit, Zhilingtou gold-silver deposit and Haoshi silver deposit) showmarked differences in lead and strontium isotopic composition, suggesting three differentsources and geneses of these deposits. The Huangshan gold deposit features low initial Srisotope ratios and low μ values or low content of radiogenic Pb and its ore-forming materialscame primarily from the upper mantle; the Zhilingtou gold-silver deposit shows high initial Srisotope ratios and high μ values or high content of radiogenic Pb and the ore-forming materialswere derived mainly from the upper crust; and the Haoshi silver deposit has its Pb and Srisotope ratios between the above two cases with the ore-forming materials stemming from boththe mantle and the crust. The characteristic Pb isotopic composition may serve as an indicatorfor prospecting for different types of ore deposits.展开更多
Understanding the interaction between groundwater and surface water in permafrost regions is essential to study flood frequencies and river water quality, especially in the high latitude/altitude basins. The applicati...Understanding the interaction between groundwater and surface water in permafrost regions is essential to study flood frequencies and river water quality, especially in the high latitude/altitude basins. The application of heat tracing method,based on oscillating streambed temperature signals, is a promising geophysical method for identifying and quantifying the interaction between groundwater and surface water. Analytical analysis based on a one-dimensional convective-conductive heat transport equation combined with the fiber-optic distributed temperature sensing method was applied on a streambed of a mountainous permafrost region in the Yeniugou Basin, located in the upper Heihe River on the northern Tibetan Plateau. The results indicated that low connectivity existed between the stream and groundwater in permafrost regions.The interaction between surface water and groundwater increased with the thawing of the active layer. This study demonstrates that the heat tracing method can be applied to study surface water-groundwater interaction over temporal and spatial scales in permafrost regions.展开更多
Knowledge tracing is the key component in online individualized learning,which is capable of assessing the users'mastery of skills and predicting the probability that the users can solve specific problems.Availabl...Knowledge tracing is the key component in online individualized learning,which is capable of assessing the users'mastery of skills and predicting the probability that the users can solve specific problems.Available knowledge tracing models have the problem that the assessments are not directly used in the predictions.To make full use of the assessments during predictions,a novel model,named deep knowledge tracing embedding neural network(DKTENN),is proposed in this work.DKTENN is a synthesis of deep knowledge tracing(DKT)and knowledge graph embedding(KGE).DKT utilizes sophisticated long short-term memory(LSTM)to assess the users and track the mastery of skills according to the users'interaction sequences with skill-level tags,and KGE is applied to predict the probability on the basis of both the embedded problems and DKT's assessments.DKTENN outperforms performance factors analysis and the other knowledge tracing models based on deep learning in the experiments.展开更多
文摘Ray tracing is a computer graphics method that renders images realistically. As the name suggests, this technique primarily traces the path of light rays interacting with objects in a scene [1], permitting the calculation of lighting and reflecting impact [2]. As ray tracing is a time-consuming process, the need for parallelization to solve this problem arises. One downside of this solution is the existence of race conditions. In this work, we explore and experiment with a different, well-known solution for this race condition. Starting with the introduction and the background section, a brief overview of the topic is followed by a detailed part of how the race conditions may occur in the case of the ray tracing algorithm. Continuing with the methods and results section, we have used OpenMP to parallelize the Ray tracing algorithm with the different compiler directives critical, atomic, and first-private. Hence, it concluded that both critical and atomic are not efficient solutions to produce a good-quality picture, but first-private succeeded in producing a high-quality picture.
基金supported by the National Natural Science Foundation of China,Nos.32100899(to KZL),31830035(to FQX),31771156(to FQX),21921004(to FQX)the National Science and Technology Innovation 2030,No.2021ZD0201003(to FQX)+2 种基金the Key-Area Research and Development Program of Guangdong Province,No.2018B030331001(to FQX)the Strategic Priority Research Program of the Chinese Academy of Sciences,No.XDB32030200(to FQX)the Shenzhen Key Laboratory of Viral Vectors for Biomedicine,No.ZDSYS20200811142401005(to FQX)。
文摘Analyzing the structure and function of the brain's neural network is critical for identifying the working principles of the brain and the mechanisms of brain diseases.Recombinant rabies viral vectors allow for the retrograde labeling of projection neurons and cell type-specific trans-monosynaptic tracing,making these vectors powerful candidates for the dissection of synaptic inputs.Although several attenuated rabies viral vectors have been developed,their application in studies of functional networks is hindered by the long preparation cycle and low yield of these vectors.To overcome these limitations,we developed an improved production system for the rapid rescue and preparation of a high-titer CVS-N2c-ΔG virus.Our results showed that the new CVS-N2c-ΔG-based toolkit performed remarkably:(1)N2cG-coated CVS-N2c-ΔG allowed for efficient retrograde access to projection neurons that were unaddressed by rAAV9-Retro,and the efficiency was six times higher than that of rAAV9-Retro;(2)the trans-monosynaptic efficiency of oG-mediated CVS-N2c-ΔG was 2–3 times higher than that of oG-mediated SAD-B19-ΔG;(3)CVS-N2c-ΔG could delivery modified genes for neural activity monitoring,and the time window during which this was maintained was 3 weeks;and(4)CVS-N2c-ΔG could express sufficient recombinases for efficient transgene recombination.These findings demonstrate that new CVS-N2c-ΔG-based toolkit may serve as a versatile tool for structural and functional studies of neural circuits.
基金supported by the National Natural Science Foundation of China,No.81870655(to MBY)。
文摘Successful establishment of reconnection between retinal ganglion cells and retinorecipient regions in the brain is critical to optic nerve regeneration.However,morphological assessments of retinorecipient regions are limited by the opacity of brain tissue.In this study,we used an innovative tissue cleaning technique combined with retrograde trans-synaptic viral tracing to observe changes in retinorecipient regions connected to retinal ganglion cells in mice after optic nerve injury.Specifically,we performed light-sheet imaging of whole brain tissue after a clearing process.We found that pseudorabies virus 724(PRV724)mostly infected retinal ganglion cells,and that we could use it to retrogradely trace the retinorecipient regions in whole tissue-cleared brains.Unexpectedly,PRV724-traced neurons were more widely distributed compared with data from previous studies.We found that optic nerve injury could selectively modify projections from retinal ganglion cells in the hypothalamic paraventricular nucleus,intergeniculate leaflet,ventral lateral geniculate nucleus,central amygdala,basolateral amygdala,Edinger-Westphal nucleus,and oculomotor nucleus,but not the superior vestibular nucleus,red nucleus,locus coeruleus,gigantocellular reticular nucleus,or facial nerve nucleus.Our findings demonstrate that the tissue clearing technique,combined with retrograde trans-synaptic viral tracing,can be used to objectively and comprehensively evaluate changes in mouse retinorecipient regions that receive projections from retinal ganglion cells after optic nerve injury.Thus,our approach may be useful for future estimations of optic nerve injury and regeneration.
基金The National Natural Science Foundation of China under contract Nos 41576083 and 41906150the National Key R&D Program of China under contract No.2022YFE0209300.
文摘Submarine groundwater discharge(SGD)is an important part in the land-sea interactions,which mainly contains three components:submarine fresh groundwater discharge(SFGD),tidal flat recirculated saline groundwater discharge(tidal flat RSGD)and subtidal recirculated saline groundwater discharge(subtidal RSGD).In order to make a more accurate assessment of the impact of SGD on coastal ecological environment,it is necessary to distinguish the main components of SGD.In this study,the Maowei Sea,located in the northern part of the Beibu Gulf,was selected as the study area.Based on the radium(Ra)tracing method,we present a new analytical method for distinguishing the three main components of SGD in this area combined with field data.The average daily flow along the coastline of the Maowei Sea of tidal flat RSGD was slightly higher than that of SFGD,and both two were on the magnitude of 1×10^(5)m^(3)/d.The average daily flow for the subtidal RSGD of the entire subtidal zone of the Maowei Sea reached to the magnitude of 1×10^(6)-1×10^(7)m^(3)/d.The long-term variation trend of terrestrial SGD is a valuable information for the study of the influence of terrigenous material on the coastal ecological environment.Based on the results of four sampling periods,it is found that the fluxes of SFGD and tidal flat RSGD in the Maowei Sea had good linear correlation with the net precipitation.As an example,January 2015 to August 2022 were selected as the study periods,and the variation trends of SFGD and tidal flat RSGD were calculated by linear function with net precipitation as the independent variable.The results showed that the flux of tidal flat RSGD was slightly higher than that of SFGD,and the difference between the two is larger in flood season while smaller in dry season.In general,in the coastal range of China,the total SGD flux in the Maowei Sea area is at a high level,and the SFGD flux is at a medium level.
基金supported by the Natural Science Foundation of China (62372277)the Natural Science Foundation of Shandong Province (ZR2022MF257, ZR2022MF295)Humanities and Social Sciences Fund of the Ministry of Education (21YJC630157)。
文摘With the popularity of online learning in educational settings, knowledge tracing(KT) plays an increasingly significant role. The task of KT is to help students learn more effectively by predicting their next mastery of knowledge based on their historical exercise sequences. Nowadays, many related works have emerged in this field, such as Bayesian knowledge tracing and deep knowledge tracing methods. Despite the progress that has been made in KT, existing techniques still have the following limitations: 1) Previous studies address KT by only exploring the observational sparsity data distribution, and the counterfactual data distribution has been largely ignored. 2) Current works designed for KT only consider either the entity relationships between questions and concepts, or the relations between two concepts, and none of them investigates the relations among students, questions, and concepts, simultaneously, leading to inaccurate student modeling. To address the above limitations,we propose a graph counterfactual augmentation method for knowledge tracing. Concretely, to consider the multiple relationships among different entities, we first uniform students, questions, and concepts in graphs, and then leverage a heterogeneous graph convolutional network to conduct representation learning.To model the counterfactual world, we conduct counterfactual transformations on students’ learning graphs by changing the corresponding treatments and then exploit the counterfactual outcomes in a contrastive learning framework. We conduct extensive experiments on three real-world datasets, and the experimental results demonstrate the superiority of our proposed Graph CA method compared with several state-of-the-art baselines.
文摘Parametric curves such as Bézier and B-splines, originally developedfor the design of automobile bodies, are now also used in image processing andcomputer vision. For example, reconstructing an object shape in an image,including different translations, scales, and orientations, can be performedusing these parametric curves. For this, Bézier and B-spline curves can be generatedusing a point set that belongs to the outer boundary of the object. Theresulting object shape can be used in computer vision fields, such as searchingand segmentation methods and training machine learning algorithms. Theprerequisite for reconstructing the shape with parametric curves is to obtainsequentially the points in the point set. In this study, a novel algorithm hasbeen developed that sequentially obtains the pixel locations constituting theouter boundary of the object. The proposed algorithm, unlike the methods inthe literature, is implemented using a filter containing weights and an outercircle surrounding the object. In a binary format image, the starting point ofthe tracing is determined using the outer circle, and the next tracing movementand the pixel to be labeled as the boundary point is found by the filter weights.Then, control points that define the curve shape are selected by reducing thenumber of sequential points. Thus, the Bézier and B-spline curve equationsdescribing the shape are obtained using these points. In addition, differenttranslations, scales, and rotations of the object shape are easily provided bychanging the positions of the control points. It has also been shown that themissing part of the object can be completed thanks to the parametric curves.
基金Sponsored by the National Natural Science Foundation of China(Grant No.52175491).
文摘A method was proposed to analyze the influences of the non-ideal spectroscopic performance of optical components and orientation errors of a laser tracing measurement optical system on the tracing measurement performance.A comprehensive model of the interference fringe contrast based on the laser tracing system s measurement principle was established in this study.Simulation results based on ZEMAX verified the model.According to the simulation results,the placement angle of the analyzer had a direct influence on the interference fringe contrast.When the angle of the polarized light to the analyzer’s transmission axis increased from 65°to 85°,each contrast of the four-way interference fringes decreased from 0.9996 to 0.3528,the interference fringe contrast is decreased by 65%.Under the split ratio of beam splitters in the interference part(BS 1)of 5∶5,when the splitting ratio of BS 2 changed from 2∶8 to 8∶2,the fringe contrast of the interference signals received by the photodetectors increased,but the injection light intensity onto the PSD reflected by BS 2 decreased.The significant influence of the tracing performance was verified by the experiments.When splitting ratio of BS 2 increased,the contrast of the interference fringes increased.Due to the weakening of the incident light intensity of the PSD caused by the change of BS 2 splitting ratio,the response time of the tracing system is increased by 23.7 ms.As a result,the tracing performance of the laser tracing measurement optical system was degraded.An important theoretical basis was provided to evaluate and improve the accuracy and reliability of laser tracing measurement systems.
基金supported by the National Natural Science Foundation of China(62001519)the State Key Laboratory of Advanced Rail Autonomous Operation(RCS2022ZZ004).
文摘Radio propagation environment plays a critical role in the performance of wireless communication systems,and understanding channel characteristics is vital for ensuring reliable communication links and optimizing system performance.Ray tracing is an effective method to investigate propagation characteristics in a complex environment,and how to quickly and accurately obtain environmental information needs to be solved.This paper presents dynamic environment reconstruction and ray tracing simulation in railway tunnel environment based on Simultaneous Localization and Mapping(SLAM)algorithm and Poisson reconstruction algorithm.Accurate channel parameters are obtained and analyzed based on ray tracing simulation.Both straight and curved tunnels are considered and investigated,and the results show the channel characteristics in complex railway tunnel environments.
基金This study was supported by the National Natural Science Foundation of China(No.U1801681,to GC and No.31970906,to WL)Guangdong Science and Technology Department(‘Key technologies for treatment of brain disorders’,No.2018B030332001,to GC)+2 种基金the Natural Science Foundation of Guangdong Province of China(No.2020A1515011079,to WL and No.2020A1515010854,to QW)the internal funding from Jinan University(No.21616110,to GC)the Young Scientists Fund of the National Natural Science Foundation of China(No.31701291,to WL).
文摘Regenerating functional new neurons in the adult mammalian central nervous system has been proven to be very challenging due to the inability of neurons to divide and repopulate themselves after neuronal loss.Glial cells,on the other hand,can divide and repopulate themselves under injury or diseased conditions.We have previously reported that ectopic expression of NeuroD1 in dividing glial cells can directly convert them into neurons.Here,using astrocytic lineage-tracing reporter mice(Aldh1l1-CreERT2 mice crossing with Ai14 mice),we demonstrate that lineage-traced astrocytes can be successfully converted into NeuNpositive neurons after expressing NeuroD1 through adeno-associated viruses.Retroviral expression of NeuroD1 further confirms that dividing glial cells can be converted into neurons.Importantly,we demonstrate that for in vivo cell conversion study,using a safe level of adeno-associated virus dosage(10^10–10^12 gc/mL,1μL)in the rodent brain is critical to avoid artifacts caused by toxic dosage,such as that used in a recent bioRxiv study(2×10^13 gc/mL,1μL,mouse cortex).For therapeutic purpose under injury or diseased conditions,or for non-human primate studies,adeno-associated virus dosage needs to be optimized through a series of dose-finding experiments.Moreover,for future in vivo gliato-neuron conversion studies,we recommend that the adeno-associated virus results are further verified with retroviruses that mainly express transgenes in dividing glial cells in order to draw solid conclusions.The study was approved by the Laboratory Animal Ethics Committee of Jinan University,China(approval No.IACUC-20180330-06)on March 30,2018.
文摘A new method of Neutron Activation Analysis (NAA) for sediment movement study is presented in this paper. Through analyzing the natural sediment, the information about natural tracing agents has been obtained, which can show the direction of sediment movement and relative discharge of sediment transportation. Test results in some areas, such as Shantou Harbour and Hangzhou Bay, are in good agreement with those from normal hydrologic measurements and some results are not obtainable with the latter. The NAA is economical, practical and reliable, and should be widely used in engineering practice.
基金National Natural Science Foundation of China(Grant number 61971365)National Natural Science Foundation of China(Grant number 61871339)+1 种基金National Natural Science Foundation of China(Grant number 61901403)Xiamen Science and Technology Plan Project(No.3502Z20183008).
文摘In 5G era,it is expected to achieve wireless network coverage including offshore areas.Modeling of marine wireless channels is the basis of constructing a marine communication system.In this paper,a communication scene between an unmanned aerial vehicle(UAV)and a boat is simulated to study the marine wireless channel.Firstly,an improved spatial partitioning ray tracing algorithm is proposed to track the propagation path of electromagnetic waves at sea surface.Secondly,a mobile channel is simulated and modeled based on the track results.Finally,a loss measurement is carried out in the coastal waters based on the simple wireless channel loss measuring platform,and a path loss propagation model is built.Then we compare the actual measurement data with the simulation results and find that the two are have good consistency,which further verifies the reliability of the simulation.
文摘A new algorithm for ray tracing bicubic Bezier surface intersection is presented. In order to find the intersected patches more effectively, a quadtree representation for surface patches is utilized. The introduction of the alternative binary tree subdivision of Bezier surface effectively improves the performance of the ray surface intersection, with the benefit of avoiding the problem that the binary tree subdivision would produce long narrow patches and speeding the intersection finding process. The algorithm has been programmed in FORTRAN-77 and on prime-550 II computer. The result shows that its structure is simple and it is easy to implement with good performance.
基金support of the National Natural Science Fundation of China (Nos. 41574105 and 41674118)the National Science and Technology Major Project of China (No. 2016ZX05027-002)the Scientific and Technological Innovation Project financially supported by Qingdao National Laboratory for Marine Science and Technology (No. 2015ASKJ03)
文摘The surface-related multiple elimination(SRME) method is based on feedback formulation and has become one of the most preferred multiple suppression methods used. However, some differences are apparent between the predicted multiples and those in the source seismic records, which may result in conventional adaptive multiple subtraction methods being barely able to effectively suppress multiples in actual production. This paper introduces a combined adaptive multiple attenuation method based on the optimized event tracing technique and extended Wiener filtering. The method firstly uses multiple records predicted by SRME to generate a multiple velocity spectrum, then separates the original record to an approximate primary record and an approximate multiple record by applying the optimized event tracing method and short-time window FK filtering method. After applying the extended Wiener filtering method, residual multiples in the approximate primary record can then be eliminated and the damaged primary can be restored from the approximate multiple record. This method combines the advantages of multiple elimination based on the optimized event tracing method and the extended Wiener filtering technique. It is an ideal method for suppressing typical hyperbolic and other types of multiples, with the advantage of minimizing damage of the primary. Synthetic and field data tests show that this method produces better multiple elimination results than the traditional multi-channel Wiener filter method and is more suitable for multiple elimination in complicated geological areas.
文摘For large-scale 3D seismic data,target-oriented reservoir imaging is more attractive than conventional full-volume migration,in terms of computation efficiency.Gaussian beam migration(GBM)is one of the most robust depth imaging method,which not only keeps the advantages of ray methods,such as high efficiency and flexibility,but also allows us to solve caustics and multipathing problems.But conventional Gaussian beam migration requires slant stack for prestack data,and ray tracing from beam center location to subsurface,which is not easy to be directly applied for target-oriented imaging.In this paper,we modify the conventional Gaussian beam migration scheme,by shooting rays from subsurface image points to receivers to implement wavefield back-propagation.This modification helps us to achieve a better subsurface illumination in complex structure and allows simple implementation for target reservoir imaging.Significantly,compared with the wavefi eld-based GBM,our method does not reconstruct the subsurface snapshots,which has higher efficiency.But the proposed method is not as efficient as the conventional Gaussian beam migration.Synthetic and field data examples demonstrate the validity and the target-oriented imaging capability of our method.
基金This study was supported by grants from National Key Research and Development Plan for Digital Diagnostic Equipment Research and Development(No.2016YFC0106700)the Natural Science Foundation of Union Hospital,Tongji Medical College,Huazhong University of Science and Technology(No.02.03.2018-131).
文摘Incorporation of the Monte Carlo(MC)algorithm in optimizing CyberKnife(CK)plans is cumbersome,and early models unconfigured MC calculations,therefore,this study investigated algorithm-based dose calculation discrepancies by selecting different prescription isodose lines(PIDLs)in head and lung CK plans.CK plans were based on anthropomorphic phantoms.Four shells were set at 2-60 mm from the target,and the constraint doses were adjusted according to the design stratcgy.After optimization,30%-90%PIDL plans were generated by ray tracing(RT).In the evaluation module,CK plans were recalculated using the MC algorithm.Therefore,the dosimetric parameters of different PIDL plans based on the RT and MC algorithms were obtained and analyzed.The discrepancies(mean+SD)were 3.72%+0.31%,3.40%+0.11%,3.47%+0.32%,0.17%+0.11%,0.64%+3.60%,7.73%+1.60%,14.62%+3.21%and 10.10%+1.57%for Djs,Dmeam),Dys,and coverage of the PTV,DGI,V,,V;and V,in the head plans and-6.32%+1.15%,-13.46%+0.98%,-20.63%+2.25%,-34.78%+25.03%,12248%+175.60%,-12.92%+5.41%,3.19%+4.67%and 7.13%+1.56%in the lung plans,respectively.The following parameters were significantly correlated with PIDL:dp98%at the 0.05 level and dpal,dys and dv3 at the 0.01 level for the head plans;dp98e%at the 0.05 level and do1e%,dpmeam,Ccoweange,dool,dvs and dv;at the 0.01 level for the lung plans.RT may be used to calculate the dose in CK head plans,but when the dose of organs at risk is close to the limit,it is necessary to refer to the MC results or to further optimize the CK plan to reduce the dose.For lung plans,the MC algorithm is recommended.For early models without the MC algorithm,a lower PIDL plan is recommended;otherwise,a large PIDL plan risks serious underdosage in the target area.
文摘This paper deals with characteristics of silicon isotope compositions and siliceous cathodolumines-cence of host rocks, ores and hydrothermal silicified quartz of the Carlin-type ore deposits in the Yunnan-Guizhou-Guangxi triangle area. The study shows that primary silicified quartz is nonluminescent but quartz in host rocks andsecondary silicified quartz are luminescent by the action of cathode rays. Correspondingly, silicon isotope composi-tions of host rocks, ores and hydrothermal quartz veins are clearly distinguished. In strata from the Middle Triassic tothe 'Dachang' host bed, δ<sup>30</sup>Si of the host rocks ranges from 0.0‰-0.3‰, while that of primary ore-forming silici-fied fluids from -0.1‰ to -0.4‰, in the Upper Permian and Lower Carboniferous strata and Indosinian diabasehost beds, δ<sup>30</sup>Si of the host rocks is from -0.1‰ to -0.2‰ and that of the primary silicified quartz veins from 0.3‰-0.5‰. This pattern demonstrates the following geochemical mineralization process: primary ore-forming sili-ceous fluids migrated upwards quickly along the main passages of deep-seated faults from mantle to crust and en-tered secondary faults where gold deposits were eventually formed as a result of permeation and replacement of thesiliceous ore-forming fluids into different ore-bearing strata. This gives important evidence for the fact that ore-forming fluids of this type of gold deposits were mainly derived from upper mantle differentiation and shows goodprospects for deep gold deposits and geochemical background for large and superlarge gold deposits.
文摘Three different types of gold and silver deposits in Zhejiang Province(Huangshan gold deposit, Zhilingtou gold-silver deposit and Haoshi silver deposit) showmarked differences in lead and strontium isotopic composition, suggesting three differentsources and geneses of these deposits. The Huangshan gold deposit features low initial Srisotope ratios and low μ values or low content of radiogenic Pb and its ore-forming materialscame primarily from the upper mantle; the Zhilingtou gold-silver deposit shows high initial Srisotope ratios and high μ values or high content of radiogenic Pb and the ore-forming materialswere derived mainly from the upper crust; and the Haoshi silver deposit has its Pb and Srisotope ratios between the above two cases with the ore-forming materials stemming from boththe mantle and the crust. The characteristic Pb isotopic composition may serve as an indicatorfor prospecting for different types of ore deposits.
基金supported by the National Natural Science Foundation of China(41690141,41671067)the second Tibetan Plateau Scientific Expedition and Research Program(STEP)(2019QZKK0605)+1 种基金the Fundamental Research Funds for Central Universities(lzujbky-2019-40)CAS"Light of West China"and the State Key Laboratory of Cryospheric Science,CAS(SKLCS-ZZ-2020).
文摘Understanding the interaction between groundwater and surface water in permafrost regions is essential to study flood frequencies and river water quality, especially in the high latitude/altitude basins. The application of heat tracing method,based on oscillating streambed temperature signals, is a promising geophysical method for identifying and quantifying the interaction between groundwater and surface water. Analytical analysis based on a one-dimensional convective-conductive heat transport equation combined with the fiber-optic distributed temperature sensing method was applied on a streambed of a mountainous permafrost region in the Yeniugou Basin, located in the upper Heihe River on the northern Tibetan Plateau. The results indicated that low connectivity existed between the stream and groundwater in permafrost regions.The interaction between surface water and groundwater increased with the thawing of the active layer. This study demonstrates that the heat tracing method can be applied to study surface water-groundwater interaction over temporal and spatial scales in permafrost regions.
文摘Knowledge tracing is the key component in online individualized learning,which is capable of assessing the users'mastery of skills and predicting the probability that the users can solve specific problems.Available knowledge tracing models have the problem that the assessments are not directly used in the predictions.To make full use of the assessments during predictions,a novel model,named deep knowledge tracing embedding neural network(DKTENN),is proposed in this work.DKTENN is a synthesis of deep knowledge tracing(DKT)and knowledge graph embedding(KGE).DKT utilizes sophisticated long short-term memory(LSTM)to assess the users and track the mastery of skills according to the users'interaction sequences with skill-level tags,and KGE is applied to predict the probability on the basis of both the embedded problems and DKT's assessments.DKTENN outperforms performance factors analysis and the other knowledge tracing models based on deep learning in the experiments.
